Fibre degrading enzymes and Lactobacillus plantarum influence liquid feed characteristics and the solubility of fibre components and dry matter in vitro

2007 ◽  
Vol 109 (1-3) ◽  
pp. 100-103 ◽  
Author(s):  
P. Christensen ◽  
V. Glitsø ◽  
D. Pettersson ◽  
B. Wischmann
Keyword(s):  
Lactobacillus Plantarum ◽  
Dry Matter ◽  
Degrading Enzymes ◽  
Liquid Feed

scholarly journals Application of Selected Inoculant Producing Antifungal and Fibrinolytic Substances on Rye Silage with Different Wilting Time

Processes ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 879
Author(s):  
Seong-Shin Lee ◽  
Jeong-Seok Choi ◽  
Dimas Hand Vidya Paradhipta ◽  
Young-Ho Joo ◽  
Hyuk-Jun Lee ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Dry Matter ◽  
Lactobacillus Brevis ◽  
Neutral Detergent Fiber ◽  
Dry Matter Digestibility ◽  
Fiber Digestibility ◽  
Aerobic Stability ◽  
Commercial Inoculant

This research was conducted to determine the effects of selected inoculant on the silage with different wilting times. The ryes were unwilted or wilted for 12 h. Each rye forage was ensiled for 100 d in quadruplicate with commercial inoculant (Lactobacillus plantarum sp.; LPT) or selected inoculant (Lactobacillus brevis 100D8 and Leuconostoc holzapfelii 5H4 at 1:1 ratio; MIX). In vitro dry matter digestibility and in vitro neutral detergent fiber digestibility were highest in the unwilted MIX silages (p < 0.05), and the concentration of ruminal acetate was increased in MIX silages (p < 0.001; 61.4% vs. 60.3%) by the increase of neutral detergent fiber digestibility. The concentration of ruminal ammonia-N was increased in wilted silages (p < 0.001; 34.8% vs. 21.1%). The yeast count was lower in the MIX silages than in the LPT silages (p < 0.05) due to a higher concentration of acetate in MIX silages (p < 0.05). Aerobic stability was highest in the wilted MIX silages (p < 0.05). In conclusion, the MIX inoculation increased aerobic stability and improved fiber digestibility. As a result of the wilting process, ammonia-N in silage decreased but ruminal ammonia-N increased. Notably, the wilted silage with applied mixed inoculant had the highest aerobic stability.

scholarly journals Utilization of a novel feedstuff: effects of additives on the fermentation characteristics, chemical composition and in vitro digestibility of tetraploid black locust (Robinia pseudoacacia) silage

2019 ◽  
Vol 49 (7) ◽  
Author(s):  
Zhihao Dong ◽  
Junfeng Li ◽  
Lei Chen ◽  
Siran Wang ◽  
Tao Shao
Keyword(s):  
Lactic Acid ◽  
Chemical Composition ◽  
Lactobacillus Plantarum ◽  
Dry Matter ◽  
Neutral Detergent Fiber ◽  
In Vitro Digestibility ◽  
Fermentation Products ◽  
Fermentation Quality ◽  
Tetraploid Black Locust

ABSTRACT: This study was conducted to evaluate the effects of additives on the fermentation characteristics, chemical composition and in vitro digestibility of tetraploid black locust (TBL). The TBL leaves silage was either untreated (control) or treated with 1 × 106 cfu/g FM Lactobacillus plantarum (L), 1% glucose (G), 3% molasses (M), a combination of 1% glucose and Lactobacillus plantarum (L+G), or a combination of 3% molasses and Lactobacillus plantarum (L+M). Fermentation quality, chemical composition and nutrient digestibility were then analyzed. Ethanol and acetic acid concentrations were the dominant fermentation products in all silages except L+M silage. The L, G and L+G treatments failed to influence the fermentation. The M treatment increased (P<0.05) the lactic acid concentration and lowered (P<0.05) the pH when compared with control silage. The best fermentation properties were observed in L+M silage, as indicated by the dominance of lactic acid over ethanol in fermentation products. The M and L+M silages exhibited higher (P<0.05) dry matter, and M silage showed higher residual water-soluble carbohydrates than the control. Ensiling increased (P<0.05) the in vitro dry matter, neutral detergent fiber and acid detergent fiber degradability of TBL. Among the silages, M silage had the highest levels of dry matter, neutral detergent fiber and acid detergent fiber degradability. The obtained results suggested that application of lactic acid bacteria together with 3% molasses could be an effective strategy to prevent the occurrence of ethanol fermentation and improve fermentation quality of TBL silage; addition of fermentable sugars to TBL improves nutrient availability to ruminants.

scholarly journals Influence of Fermented Diets on In Vitro Survival Rate of Some Artificially Inoculated Pathogens—A Preliminary Study

Processes ◽  
2020 ◽  
Vol 8 (11) ◽  
pp. 1345
Author(s):  
Sebastian Bunte ◽  
Birgit Keller ◽  
Bussarakam Chuppava ◽  
Josef Kamphues ◽  
Christian Visscher ◽  
...  
Keyword(s):  
Survival Rate ◽  
Dry Matter ◽  
Starter Culture ◽  
In Vitro Study ◽  
Pathogen Transmission ◽  
Post Inoculation ◽  
Liquid Feed ◽  
Serovar Typhimurium ◽  
Preliminary Study

Improving the hygienic status of feed ingredients by biotechnological processes as fermentation is of the greatest concern. This preliminary study aimed to investigate whether there are relevant effects of fermented liquid feed (FLF) on the survival of potential pathogens in vitro. The feed (fresh basis) consisted of 50% rye, 30% rapeseed extracted meal, 10% barley and 10% wheat. Glass bottles were filled about 14.1 g water (38 °C) containing the diluted starter culture and feed (8.81 g). Fermentation led to high levels of lactate (5–7% of dry matter), low pH values (<4.0) and low levels of acetic acid (<1% of dry matter) in the FLF. The survival rate of pathogens added, such as Salmonella enterica serovar Typhimurium, Escherichia coli and Clostridium perfringens after 6 h of controlled fermentation, was significantly reduced (<2 log10 CFU/g). The counts of Candida krusei in FLF at 3 h and 6 h post inoculation remained almost unchanged regardless of the incubation time. Even adding sodium-benzoate at a concentration of up to 0.25% in the liquid feed did not reduce the survival of C.krusei during fermentation. Based on this in vitro study, feeding of FLF seems a promising strategy to reduce pathogen transmission but has to be confirmed on natural feeds by pathogens for increasing the hygienic properties.

scholarly journals Effects of wilting and additives on the ensiling quality and in vitro rumen fermentation characteristics of sudangrass silage

2021 ◽  
Vol 34 (1) ◽  
pp. 56-65
Author(s):  
Jiang Chun Wan ◽  
Kai Yun Xie ◽  
Yu Xiang Wang ◽  
Li Liu ◽  
Zhu Yu ◽  
...  
Keyword(s):  
Production Rate ◽  
Lactobacillus Plantarum ◽  
Dry Matter ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Nutrient Digestibility ◽  
Ruminal Fermentation ◽  
Fresh Matter ◽  
Positive Effects

Objective: This study was conducted to investigate the effects of molasses and <i>Lactobacillus plantarum</i> on the ensiling quality and <i>in vitro</i> rumen fermentation of sudangrass silage prepared with or without wilting.Methods: The ensiling experiment, measured with 3 replicates, was carried out according to a 2×4 (wilted stages×additives) factorial treatment structure. Dry matter of the fresh (210 g/kg fresh matter) or wilted (305 g/kg fresh matter) sudangrass were ensiled (packed into 5.0-L plastic jars) without additive (control) or with molasses (M), <i>Lactobacillus plantarum</i> (LP), or molasses + <i>Lactobacillus plantarum</i> (M+LP). After 60 days of ensiling, the silages were analyzed for the chemical, fermentation, and <i>in vitro</i> characteristics.Results: After 60 days of ensiling, the fermentation parameters were affected by wilted, the additives and the interactions of wilted with the additives (p<0.05). The M+LP treatment at wilted had higher lactic acid levels and V-score (p<0.05) but lower pH values and butyric acid concentrations than the other treatments. In comparison with sudangrass before ensiling, after ensiling had lower dry matter and higher non-fibrous carbohydrate. The <i>in vitro</i> gas production, <i>in vitro</i> dry matter digestibility, <i>in vitro</i> crude protein digestibility, and <i>in vitro</i> acid fiber detergent digestibility changed under the effects of the additives. Significant interactions were observed between wilted and the additives in terms of <i>in vitro</i> gas production at 48 h, asymptotic gas production, gas production rate, half time, and the average gas production rate. The total volatile fatty acid levels in the additive treatments were higher than those in the control.Conclusion: Wilting and supplementation with molasses and <i>Lactobacillus plantarum</i> had the ability to improve the ensiling quality and <i>in vitro</i> nutrient digestibility of sudangrass silage. The M+LP treatment at wilted exhibited the strongest positive effects on silage quality and <i>in vitro</i> ruminal fermentation characteristics.

scholarly journals PSI-22 Effects of supplementing sugar beet processing by-products in low-quality forage diets on in vitro fermentation characteristics and dry matter digestibility

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 248-249
Author(s):  
Glory M Ogren ◽  
Gwinyai Chibisa
Keyword(s):  
Sugar Beet ◽  
Dry Matter ◽  
Ruminal Fermentation ◽  
In Vitro Fermentation ◽  
Molar Proportion ◽  
Dry Matter Digestibility ◽  
Liquid Feed ◽  
Inclusion Level ◽  
By Products

Abstract Sugar beet processing by-products can be used to increase nutrient supply in beef cattle fed low-quality forages. However, information on the effectiveness of the different byproducts on modulating ruminal fermentation is still limited. Our objective was to determine the effects of providing supplemental molasses, concentrated seperator by-product, liquid feed-grade (32% purity) and crystalline betaine (97%) on in vitro fermentation and dry matter digestibility (IVDMD). A batch culture system was used to evaluate volatile fatty acid (VFA) production (3 h), pH (24 and 48 h), and IVDMD (24 and 48 h). A low-quality hay (7.5% crude protein; 58% total digestible nutrients) was used as a substrate and the inclusion level was 10% (dry matter [DM] basis) for all by-products except for crystalline betaine. Because it is a solid, a solution of crystalline betaine was made such that the betaine inclusion level was similar to that of feed-grade betaine. Data was analyzed using PROC MIXED in SAS. Total VFA concentration was lower (P &lt; 0.01) for crystalline and liquid betaine compared to molasses and concentrated seperator by-product. Molar proportion of propionate was lower for concentrated seperator by-product (P = 0.04) than for the other treatments whereas the molar proportion of butyrate was lower (P = 0.04) for crystal betaine compared to molasses and concentrated seperator by-product. However, although pH was lower (P &lt; 0.01) at 48 than 24 h, whereas DMD was greater (P &lt; 0.01) at 48 than 24 h, there was no treatment effect (P ≥ 0.18) on pH and in vitro dry matter digestibility (DMD) at both 24 and 48 h. In summary, although adding different by-products from sugar beet processing resulted in changes in the VFA profile at 3 h of in vitro incubation, it had no effect on 24 and 48 h pH and IVDMD.

scholarly journals Fermentation of liquid feed with lactic acid bacteria reduces dry matter losses, lysine breakdown, formation of biogenic amines and phytate-phosphorus

2022 ◽  
Author(s):  
N Lau ◽  
J Hummel ◽  
E Kramer ◽  
M Hünerberg
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Biogenic Amines ◽  
Dry Matter ◽  
Canola Meal ◽  
P Availability ◽  
Liquid Feed ◽  
Complete Breakdown ◽  
The Impact

ABSTACT This study investigated the fermentation of liquid feed for pigs and the effect of lactic acid bacteria (LAB) supplementation on fermentation rate, dry matter losses (DML), formation of biogenic amines and degradation of phytate-P. The basal substrate in all three in-vitro batch experiments consisted of 50% canola meal, 25% wheat, and 25% barley. The mixed substrates were adjusted to a dry matter (DM) content of 28.4% and fermented in 1L-vessels at 37° C for 24 h. Experiment 1 focused on changes in pH profiles over time. Treatments were: (1) liquid feed without additive (control) and (2) liquid feed supplemented with a mixture of Lactobacillus plantarum, Pediococcus pentosaceus, and Lactobacillus lactis (adLAB) at 2.0 × 10 5 CFU/g liquid feed (wet wt.; n = 8). Substrate pH was measured every 2 h. Experiment 2 focused on DML and the impact of fermentation on phytate-P. Treatments were identical to experiment 1 (control and adLAB; n = 8). Measured parameters included concentration of lactic acid, acetic acid, ethanol, and phytate-P, and DML after 24 h of fermentation. Counts of molds, Enterobobacteriaceae, yeasts and LAB were determined in one combined sample of all replicates. Dry matter losses were lower in LAB supplemented fermentations (5.89%) compared to the control (11.8%; P &lt; 0.001). Supplementation with LAB reduced the phytate-P content (2.66 g/kg DM) compared to the control (3.07 g/kg DM; P = 0.002). Experiment 3 evaluated DML and the impact of fermentation on formation of biogenic amines. Treatments were: (1) control, (2) adLAB (2.0 × 10 5 CFU LAB/g liquid feed), (3) adLys (0.60% DM supplemented lysine) and (4) adLAB+Lys (combination of adLAB and adLys; n = 8). The fermentation of adLys resulted in a nearly complete breakdown of supplemented lysine, while only 10% of supplemented lysine was lost in adLAB+Lys. Furthermore, all adLys samples tested positive for cadaverine (mean concentration 0.89% DM), while no adLAB samples contained cadaverine above the detection limit (P &lt; 0.001). Results indicate that DML is reduced in fermentations supplemented with homofermentative LAB. Fermentation of liquid feed with homofermentative LAB can effectively reduce the degradation of supplemental lysine, and has the potential to further improve P availability.

scholarly journals Kemampuan Susu Fermentasi Lactobacillus plantarum Menghambat Salmonella typhymurium Secara In Vitro

2010 ◽  
Vol 10 (2) ◽  
pp. 34-39
Author(s):  
Zuraida Hanum
Keyword(s):  
Lactobacillus Plantarum ◽  
Dry Matter ◽  
Room Temperature ◽  
Fermented Milk ◽  
Activity Test ◽  
Milk Fermentation ◽  
Temperature Observation ◽  
Plain Paper ◽  
Paper Disc

The ability of Lactobacillus plantarum fermented milk to inhibit Salmonella typhymurium in vitroABSTRACT. This research used Lactobacillus plantarum as a milk starter (concentration 3, 4, 5 %) and incubated for 48 hours at room temperature. Observation fermented begin from the first day of this product until 7 days and its still stored at room temperature. First analysis conducted on milk in this research were Storch, pH, acidity titration, fat, and dry matter. Milk fermentation analysis of Lactobcillus plantarum including pH, degree of dornik acidity and microbe activity test. Experimental design used is Repeated Measurement with three replications. Data colected analyzed by ANOVA test. If there is significant different between treatments, followed by Least Significant Test. Complete pasteurization result test (Storch) found that milk stay white, it means that peroksidase enzyme completely disappear and milk completely pasteurization. The ability of suppressing observed by Salmonella typhymurium in Nutrient Agar and challenge with Lactobacillus plantarum fermented milk 3%, 4% and 5% (50 μl/well). Tetracyline, chloramphenicol and a plain paper disc are used as control. Lactobacillus plantarum fermented milk of starter concentration 5%-first day has the biggest inhabitation zone by Salmonella typhimurium (9.39 mm). Range of pH showed between 4.84 to 4.14 and the acidity between 114.67 0D to 365.67 0D. Sensitivity test showed that Salmonella typhymurium more sensitive than chloramphenicol and tetracyline antibiotic.

The role of silicon in forages: A quantitative X-Ray study

1987 ◽  
Vol 45 ◽  
pp. 416-417
Author(s):  
Janet H. Woodward ◽  
D. E. Akin
Keyword(s):  
Sodium Acetate ◽  
Dry Matter ◽  
Acetate Buffer ◽  
Plant Tissues ◽  
Sodium Acetate Buffer ◽  
X Ray ◽  
Dry Matter Digestibility ◽  
Percent Composition

Silicon (Si) is distributed throughout plant tissues, but its role in forages has not been clarified. Although Si has been suggested as an antiquality factor which limits the digestibility of structural carbohydrates, other research indicates that its presence in plants does not affect digestibility. We employed x-ray microanalysis to evaluate Si as an antiquality factor at specific sites of two cultivars of bermuda grass (Cynodon dactvlon (L.) Pers.). “Coastal” and “Tifton-78” were chosen for this study because previous work in our lab has shown that, although these two grasses are similar ultrastructurally, they differ in in vitro dry matter digestibility and in percent composition of Si.Two millimeter leaf sections of Tifton-7 8 (Tift-7 8) and Coastal (CBG) were incubated for 72 hr in 2.5% (w/v) cellulase in 0.05 M sodium acetate buffer, pH 5.0. For controls, sections were incubated in the sodium acetate buffer or were not treated.

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