scholarly journals Chronologic comparison of root dentin moisture in extracted human teeth stored in formalin, sodium azide, and distilled water

2009 ◽  
Vol 108 (1) ◽  
pp. e50-e54 ◽  
Author(s):  
Takashi Komabayashi ◽  
Chul Ahn ◽  
Song Zhang ◽  
Qiang Zhu ◽  
Larz S.W. Spångberg
Keyword(s):  
Sodium Azide ◽  
Distilled Water ◽  
Human Teeth ◽  
Root Dentin

scholarly journals Effect of different EGTA concentrations on dentin microhardness

2002 ◽  
Vol 13 (3) ◽  
pp. 188-190 ◽  
Author(s):  
Antonio Miranda da Cruz-Filho ◽  
Eduardo Augusto de Paula ◽  
Jesus Djalma Pécora ◽  
Manoel Damião de Sousa-Neto
Keyword(s):  
Acetic Acid ◽  
Statistical Analysis ◽  
High Speed ◽  
Acrylic Resin ◽  
Distilled Water ◽  
Three Solutions ◽  
Human Teeth ◽  
Tukey Test ◽  
Significant Difference ◽  
Root Dentin

The effect of 1%, 3% and 5% EGTA (ethylene glycol-bis-(b-amino-ethyl ether) N,N,N´,N´-tetra-acetic acid) on the microhardness of root dentin of the cervical third of human teeth was studied. Five newly extracted maxillary incisors were sectioned transversely at the cementoenamel junction, and the crowns were discarded. The roots were embedded in blocks of high-speed polymerized acrylic resin and cut transversely into 1-mm sections. The second section of the cervical third of the root of each tooth was sectioned and divided into four parts. Each part was placed on an acrylic disc that was used as a base for microhardness measurement. Fifty microliters of 1% EGTA, 3% EGTA, or 5% EGTA were applied to the dentin surface. Deionized and distilled water was used as control. Dentin microhardness was then measured with a load of 50 g for 15 s. Statistical analysis showed that the three concentrations of the chelating solution EGTA significantly reduced dentin microhardness when compared with water (ANOVA, p<0.01), and that there was a statistically significant difference among the three solutions (Tukey test, p<0.05).

Evaluation of the effect of cetrimide with edta on Microhardness and surface changes in root dentin – An invitro study

2019 ◽  
Vol 11 (1) ◽  
pp. 3-11
Author(s):  
Dr. Swarooparanil Patil ◽  
Dr. B.S. Keshava Prasad
Keyword(s):  
Surface Erosion ◽  
Distilled Water ◽  
Test Results ◽  
Chi Square ◽  
Chi Square Test ◽  
Root Dentin ◽  
Group 3 ◽  
Post Hoc ◽  
Hardness Values ◽  
Surface Changes

The aim of the study was to evaluate the effect of different concentrations of cetrimide with or without EDTA solution on the microhardness and surface changes in root dentin. Single rooted human mandibular premolar teeth were decoronated and sectioned longitudinally. The root segments were horizontally embedded in autopolymerizing resin. These specimens were randomly divided into 6 experimental groups according to the irrigating solution used. The irrigating solutions used were 5% EDTA, 5%EDTA + 0.25% Cetrimide, 5%EDTA + 0.50% Cetrimide, 0.25% Cetrimide, 0.50% Cetrimide and distilled water. Each group was further divided into two subgroups. First subgroup specimens were employed for microhardness testing using vicker’s indenter. Second subgroup specimens were used to evaluate the surface changes of root dentin under stereomicroscope. Comparison of the mean microhardness of test groups was done using ANOVA followed by post hoc Tukey’s test. Pre- and post- treatment hardness values were compared using student paired t test. The data obtained from surface erosion scoring was subjected to statistical analysis using Chi square test. Results of this study showed significant reduction in the microhardness of dentin for all the groups except for the distilled water (p<0.001). The greatest reduction in dentin microhardness was observed with Group-3 (5% EDTA + 0.50% CTR). Specimens in 5% EDTA group showed greater erosion than other groups. Within the limitations of this study it was concluded that all the tested irrigating solutions reduced the microhardness of root dentin except distilled water. Maximum reduction in microhardness was observed with addition of 0.50% cetrimide to EDTA. EDTA increased the surface roughness of root dentin irrespective of cetrimide association. Use of cetrimide at concentrations higher than 0.25% would be detrimental to the microhardness of dentin.

scholarly journals Comparison of resin push-out strength to root dentin of bovine- and human-teeth

2009 ◽  
Vol 20 (3) ◽  
pp. 332 ◽  
Author(s):  
LuizFelipe Valandro ◽  
MarcoAntonio Bottino ◽  
Graziela Galhano ◽  
RenataMarques de Melo
Keyword(s):  
Human Teeth ◽  
Root Dentin ◽  
Push Out

An in-vitro Comparison of the Bleaching Efficacy of 35 Percent Carbamide Peroxide with Established Intracoronal Bleaching Agents

2020 ◽  
Author(s):  
Nandita Bansal
Keyword(s):  
Hydrogen Peroxide ◽  
Sodium Perborate ◽  
Distilled Water ◽  
Carbamide Peroxide ◽  
Human Teeth ◽  
Anterior Teeth ◽  
Group I ◽  
Group Ii ◽  
Control Evaluation

Introduction: Intracoronal bleaching is the simplest, least invasive, and least expensive means available to lighten non-vital discolored teeth. The objective of the study: To evaluate the intra coronal bleaching efficacy of 35% Carbamide peroxide relative to 35% Hydrogen peroxide and Sodium perborate using artificially stained extracted human teeth. Materials and Methods: Eighty extracted human permanent maxillary and mandibular anterior teeth and single-rooted premolars were artificially stained using whole human blood and root canal treatment was completed. They were bleached twice 7 days apart using: Group I (n=20) – 35% Carbamide peroxide gel, Group II (n=20) – 35% Hydrogen peroxide gel, Group III (n=20) – 2 gm Sodium perborate mixed with per ml of distilled water, Group IV (n=20)– Distilled water only (control). Evaluation of shade was performed on day 0,7,14. Results: On day 7, In Groups I and Group II, there was a reduction in the shade by 2 vita tab positions, whereas Group IIIlightened by 1-tab position only. On day 14, CP and HP lightened by overall 3-tab positions and SP by 2-tab positions. There were no statistical differences between the groups on day 7 and day 14. Conclusion: 35% Carbamide peroxide, 35% Hydrogen peroxide and Sodiumperborate were equally effective for Intra coronal bleaching.

scholarly journals Effect of Gamma Radiation and Endodontic Treatment on Mechanical Properties of Human and Bovine Root Dentin

2016 ◽  
Vol 27 (6) ◽  
pp. 670-674 ◽  
Author(s):  
Veridiana Resende Novais ◽  
Priscilla Barbosa Ferreira Soares ◽  
Carlla Martins Guimarães ◽  
Laís Rani Sales Oliveira Schliebe ◽  
Stella Sueli Lourenço Braga ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Flexural Strength ◽  
Gamma Radiation ◽  
Hardness Test ◽  
Knoop Hardness ◽  
Endodontic Treatment ◽  
Bending Test ◽  
Human Teeth ◽  
Significant Difference ◽  
Root Dentin

Abstract This study evaluated the effect of gamma radiation and endodontic treatment on the microhardness and flexural strength of human and bovine root dentin. Forty single-rooted human teeth and forty bovine incisor teeth were collected, cleaned and stored in distilled water at 4 °C. The human and bovine teeth were divided into 4 groups (n=10) resulting from the combination of two study factors: first, regarding the endodontic treatment in 2 levels: with or without endodontic treatment; and second, radiotherapy in two levels: with or without radiotherapy by 60 Gy of Co-60 gamma radiation fractioned into 2 Gy daily doses five days per week. Each tooth was longitudinally sectioned in two parts; one-half was used for the three-point bending test and the other for the Knoop hardness test (KHN). Data were analyzed by 3-way ANOVA and Tukey HSD test (α=0.05). No significant difference was found for flexural strength values. The human dentin had significantly higher KHN than the bovine. The endodontic treatment and radiotherapy resulted in significantly lower KHN irrespective of tooth origin. The results indicated that the radiotherapy had deleterious effects on the microhardness of human and bovine dentin and this effect is increased by the interaction with endodontic therapy. The endodontic treatment adds additional negative effect on the mechanical properties of radiated tooth dentin; the restorative protocols should be designed taking into account this effect.

scholarly journals Assessment of Nitrofurantoin as an Experimental Intracanal Medicament in Endodontics

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Mewan Salahalddin A. Alrahman ◽  
Bestoon Muhammed Faraj ◽  
Kawa F. Dizaye
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Antibacterial Agent ◽  
Antibacterial Agents ◽  
Distilled Water ◽  
Human Teeth ◽  
Periapical Lesions ◽  
Complete Eradication ◽  
Colony Forming Unit ◽  
Triple Antibiotic Paste

Background and Objectives. Multiple antibacterial agents have been mixed and used as an intracanal medicament-like modified triple antibiotic paste (MTAP) to eliminate Enterococcus faecalis (EF), which has been most frequently identified in the cases of failed root canal treatment and periapical lesions. This study is aimed at using a single antibacterial agent, nitrofurantoin (Nit), as an experimental intracanal medicament paste against different clinical isolates of EF bacteria and at comparing its antimicrobial efficacy with MTAP. Materials and Methods. Three strains of EF (S1, S2, and S3) were clinically isolated. A total of 198 straight single-rooted human teeth were collected and divided randomly into three main groups: group N (Nit) (n=90), group M (MTAP) (n=90), and group W (distilled water) (n=18). The main groups were subdivided into three subgroups according to the strain of EF: in groups N and M, subgroups S1, S2, and S3 (n=30), while in group W, subgroups S1, S2, and S3 (n=6). Then, each subgroup of N and M was divided into five groups (n=6) according to the concentrations of Nit or MTAP (6.25, 12.5, 25, 50, and 100 mg/mL). The colony-forming unit (CFU) of EF from the canal lumen and dentinal chips was measured. Results. Nit could eradicate S1, S2, and S3 completely with concentrations of 6.25, 12.5, and 25 mg/mL, respectively, while MTAP showed complete eradication of the three strains only at 25 mg/mL. In all the groups, it was found that the CFU counts of EF in the dentinal chips were higher than those in the root canal lumen. Conclusion. At the concentration of 25 mg/mL, the Nit paste is effective in eradicating EF completely when it is used as an intracanal medicament.

scholarly journals Sterilization methods for humam teeth

2020 ◽  
Vol 68 ◽  
Author(s):  
Rafael Jordão Storino Vaz MONTEIRO ◽  
Bárbara Daphini Matos SILVA ◽  
Lorena Esteve SILVEIRA ◽  
Maria Eugênia ALVAREZ-LEITE ◽  
Flavio Ricardo MANZI ◽  
...  
Keyword(s):  
Microwave Irradiation ◽  
Brain Heart Infusion ◽  
Positive Control ◽  
Distilled Water ◽  
Pathogenic Microorganisms ◽  
Friedman Test ◽  
Human Teeth ◽  
Before And After ◽  
Sterilization Process ◽  
Potentially Pathogenic Microorganisms

ABSTRACT Appropriate sterilization methods for dental substrate are necessary so that extracted teeth can be used in laboratory activities, since potentially pathogenic microorganisms may be present even if teeth have been cleaned. Objective Evaluating the sterilization process of human teeth performed by microwave irradiation and autoclave sterilization. Methods Fifty human premolar teeth were sectioned in two portions and distributed in two test groups: G1 - autoclave sterilization for 20 minutes, 1Kgf/cm2, at 127ºC; G2 - Microwave irradiation in a recipient containing 100ml of distilled water, 900W for 5 minutes. Before and after the decontamination process, all teeth were immersed in Brain Heart Infusion (BHI) broth to verify their contamination (positive control) and decontamination potential respectively. Results The statistical analysis of the Friedman test (p <0.05) showed that all the tested sterilization methods were statistically effective. Conclusion These results show that both autoclaving and microwave irradiation are effective alternatives for the sterilization of human teeth.

scholarly journals Chitosan: effect of a new chelating agent on the microhardness of root dentin

2012 ◽  
Vol 23 (3) ◽  
pp. 212-217 ◽  
Author(s):  
Josilaine Amaral Pimenta ◽  
Danilo Zaparolli ◽  
Jesus Djalma Pécora ◽  
Antonio Miranda Cruz-Filho
Keyword(s):  
Citric Acid ◽  
Chelating Agent ◽  
Acrylic Resin ◽  
Knoop Hardness ◽  
Distilled Water ◽  
Water Control ◽  
Three Solutions ◽  
All Solutions ◽  
Root Dentin ◽  
One Way Anova

The effect of solutions of 0.2% chitosan, 15% EDTA and 10% citric acid on the microhardness of root dentin was evaluated comparatively in this study. Thirteen sound human maxillary central incisors were selected and decoronated at the cementoenamel junction. Ten roots were set into rapid polymerization acrylic resin and the root/resin block was fitted to the cutting machine to obtain slices from the cervical third. The first slice was discarded and the second slice was divided into four quadrants. Each quadrant was used to construct a sample, so that 4 specimens were obtained from each root slice, being one for each chelating solution to be tested: 15% EDTA, 10% citric acid, 0.2% chitosan and distilled water (control). The specimens were exposed to 50 μL of the solution for 5 min, and then washed in distilled water. A microhardness tester (Knoop hardness) with a 10 g load was used for 15 s. Data were analyzed statistically by one-way ANOVA and Tukey-Kramer test (α=0.05). The other 3 roots had the canals instrumented and irrigated at the end of the biomechanical preparation with the test solutions, and then examined by scanning electron microscopy (SEM) for qualitative analysis. All solutions reduced the microhardness of root dentin in a way that was statistically similar to each other (p>0.05) but significantly different from the control (p>0.05). The SEM micrographs showed that the three solutions removed smear layer from the middle third of the root canal. In conclusion, 0.2% chitosan, 15% EDTA and 10% citric acid showed similar effects in reducing dentin microhardness.

CHEMICAL MUTAGENESIS IN TWO Cuphea SPECIES

1987 ◽  
Vol 67 (3) ◽  
pp. 909-917 ◽  
Author(s):  
T. A. CAMPBELL
Keyword(s):  
Sodium Azide ◽  
Lauric Acid ◽  
Ethyl Methanesulfonate ◽  
Chemical Mutagenesis ◽  
Mutation Rates ◽  
Distilled Water ◽  
Medium Chain Triglycerides ◽  
Medium Chain ◽  
Quantitative Characters ◽  
Short Internodes

Presoaked (24 h at 20 °C) seeds of Cuphea tolucana Peyr. and C. wrightii A. Gray were treated with mutagens at 20 °C in two experiments. Experiment 1 treatments were: distilled water (DW), 0.05 M PO4 buffer (pH 7), 0.01 M ethyl methanesulfonate (EMS) (each applied for 8 h), 0.02 M EMS (applied for 4 h), 0.04 EMS M, 0.08 M EMS, or 0.16 M EMS (each applied for 2 h). Experiment 2 treatments were: DW, 0.1 M PO4 buffer (pH 3), 0.0005 M sodium azide (SA), 0.001 M SA, or 0.002 M SA (each applied for 2 h). None of the treatments had significant effects on emergence and height of M1 plants nor were any macro-mutations noted in the M2 generations. In a third experiment, DW, 0.04 M EMS, or 0.001 M SA were applied for 2 h at 30 °C to presoaked (48 or 72 h at 30 °C) seeds of C. tolucana and C. wrightii. Compared to EMS, SA had deleterious effects on height in the M1, emergence was better for C. tolucana than for C. wrightii, and C. wrightii plants grew taller after a 72-h pre-soak than after a 48-h presoak. M2 progenies were evaluated in the field. None of the presoak-treatment combinations increased variation significantly in several quantitative characters, no macro-mutations were detected in C. wrightii, but a fertile, small-leaved, decumbent mutant with very short internodes was noted in C. tolucana. Mutation rates were greatest for the 72 h presoak-EMS combination.Key words: Cuphea tolucana, Cuphea wrightii, ethyl methanesulfonate, sodium azide, medium-chain triglycerides, lauric acid

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