Consumption of nutritional pellets with Duddingtonia flagrans fungal chlamydospores reduces infective nematode larvae of Haemonchus contortus in faeces of Saint Croix lambs

2016 ◽  
Vol 91 (6) ◽  
pp. 665-671 ◽  
Author(s):  
L. Aguilar-Marcelino ◽  
P. Mendoza-de-Gives ◽  
G. Torres-Hernández ◽  
M.E. López-Arellano ◽  
C.M. Becerril-Pérez ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Repeated Measures ◽  
Body Condition Score ◽  
Treated Group ◽  
Control Group ◽  
Feed Conversion ◽  
Duddingtonia Flagrans ◽  
Time Model ◽  
Larval Population ◽  
Saint Croix

AbstractTwo groups of six Haemonchus contortus infected Saint Croix lambs each received different diets for 11 weeks: control group, commercial food, molasses and lucerne hay; and treated group, nutritional pellets (NPs) containing Duddingtonia flagrans at 2 × 106 chlamydospores/kg body weight (BW), sorghum and lucerne hay. Mean BW gain (BWG), body condition score (BCS) and packed cell volume (PCV) and also eggs/g of faeces (EPG) and recovered L3 were compared using a repeated measures across time model. Groups had similar BWG (control 139.7 ± 0.035 g/day and treated 167.7 ± 0.041 g/day), BCS (control 3.6 ± 0.39 and treated 3.4 ± 0.46) and PCV (control 32.5 ± 1.68% and treated 30.0 ± 1.68%). The mean EPG of the control group was 1215 ± 1040 and in the treated group it was 2097.91 ± 2050. No reduction in larval population was observed during weeks 2 and 3. The greatest larval population reduction in the faeces of treated lambs was observed during the first week (70.5%) and from weeks 6 to 11, with a mean value close to 70% (P < 0.05). In general, both experimental groups showed a similar feed conversion. It was concluded that both diets resulted in similar lamb growth, PCV, BCS and H. contortus EPG. However, NP consumption significantly reduced the H. contortus L3 population in lamb faeces.

scholarly journals Lack of response to garlic fed at different dose rates for the control of Haemonchus contortus in Merino wether lambs

2009 ◽  
Vol 49 (12) ◽  
pp. 1093 ◽  
Author(s):  
V. J. Strickland ◽  
J. S. Fisher ◽  
W. T. Potts ◽  
G. W. Hepworth
Keyword(s):  
Feed Intake ◽  
Haemonchus Contortus ◽  
Body Condition Score ◽  
Control Group ◽  
Feed Conversion ◽  
Worm Count ◽  
Treatment Groups ◽  
Dose Rates ◽  
Voluntary Feed Intake ◽  
Over Time

With the increased incidence of parasite resistance to chemical anthelmintics worldwide novel approaches to manage parasite infection, such as medicinal plants and their extracts, are being investigated by the scientific community. The current study tested the effect of three rates of garlic (0.9, 1.8 and 3.6%) in a pelleted ration on Haemonchus contortus in sheep. Thirty-nine Merino wether lambs aged 6 months were divided into five treatment groups, including three garlic dose rates and two control groups that received no garlic. All animals were infected with 4000 L3 H. contortus larvae 3 weeks after allocation to treatments. A positive control group was drenched with abamectin 28 days after infection. The synthetic drench was effective in controlling the parasites, but there was no reduction in either worm egg counts (WEC) or total worm count due to the garlic. The 3.6% garlic treatment had significantly lower (P < 0.05) liveweight, feed intake, body condition score and feed conversion ratio than any of the other treatment groups, suggesting that this level of garlic had a low level of anti-nutritional properties. There was an interaction between faecal WEC and voluntary feed intake over time, with the animals with higher voluntary feed intake having lower WEC over time.

Effect of active or passive immunization against steroids on reproductive performance of ewes in different levels of body condition at mating

1986 ◽  
Vol 43 (2) ◽  
pp. 285-292 ◽  
Author(s):  
S. M. Rhind ◽  
B. A. Morris ◽  
Jill Clayton ◽  
J. M. Doney ◽  
R. G. Gunn ◽  
...  
Keyword(s):  
Body Condition ◽  
Reproductive Performance ◽  
Body Condition Score ◽  
Passive Immunization ◽  
Treated Group ◽  
Ovulation Rate ◽  
Control Group ◽  
Absolute Increase ◽  
Wide Range ◽  
Antibody Titres

ABSTRACTBorder Leicester × Scottish Blackface (Greyface) ewes of three groups, each comprising 118 animals in a wide range of body condition scores, were mated at a synchronized oestrus in mid October. The ewes were passively immunized against testosterone (group P), actively immunized against androstenedione (group F), or not treated (group C). All ewes were slaughtered at return to service or at 35 to 45 days of pregnancy and ovulation rates and numbers of embryos present were determined. Mean ovulation rates of ewes in group P were higher than in those in group C (P < 0·05) and this difference was evident at most levels of body condition. The absolute increase in ovulation rate, compared with the control group, was similar at all condition scores. Mean ovulation rates of ewes in group F were higher than those in group C (P < 0·001) and the magnitude of the increase was greater in ewes in higher condition scores. The incidence of ova wastage was variable but differences between treatments in mean ovulation rate were generally reflected in mean litter size. The conception rates of immunized ewes were depressed compared with those of control animals, particularly in ewes with a body condition score less than 3·0 at mating. Consequently, there was no improvement in the potential lambing rate of immunized ewes following only one cycle of mating. Circulating antibody titres were not related to conception rate or body condition at mating and were related to ovulation rate only in group F ewes. It is concluded that immunization against steroids, using either passive or active techniques, can improve the reproductive performance of individual ewes but improvement in the performance of the flock as a whole may only be achieved under optimal conditions of nutrition and season.

scholarly journals Effect of piglet castration with nonsurgical sterilant on the zootechnical performance and pork carcass quality

2016 ◽  
Vol 68 (6) ◽  
pp. 1487-1496 ◽  
Author(s):  
D.S. Lucas ◽  
E.T.F. Siqueira ◽  
M.M.H. Haguiwara ◽  
S.S. Azevedo ◽  
S.E. Yotsuyanagi ◽  
...  
Keyword(s):  
Weight Gain ◽  
Meat Quality ◽  
Treated Group ◽  
Control Group ◽  
Active Principle ◽  
Carcass Quality ◽  
Feed Conversion ◽  
Zinc Gluconate ◽  
Hormone Levels ◽  
Significant Difference

ABSTRACT A non-surgical sterilant (NSS) was used with the objective of assessing the zootechnical performance, carcass and meat quality, and hormone levels. 90 male piglets were selected with birthweights varying between 1.5kg to 2.0kg. The control group was constituted of 45 males castrated by the conventional surgical method, at the seventh day of age, and the treated group was constituted of 45 males castrated with NSS (active principle of zinc gluconate in the concentration of 26.2mg/mL associated to the dimethyl sulfoxide at 0.5%), with the application of the first dose on the seventh day of age and the second dose on the fourteenth day of age. The zootechnical assessments were carried out on the farm and consisted: weight gain in the periods per animal and feed conversion. The slaughter of the animals and the assessment of the carcass and meat quality and hormone levels were carried out in a slaughterhouse and the municipality of Campinas (SP). The study demonstrated that the use of NSS was a viable alternative in relation to the zootechnical performance and the carcass quality. In relation to the meat quality there was no significant difference in the majority of the assessed parameters.

scholarly journals Long-Term Testosterone Therapy Improves Cardiometabolic Function and Reduces Risk of Cardiovascular Disease in Men with Hypogonadism

2017 ◽  
Vol 22 (5) ◽  
pp. 414-433 ◽  
Author(s):  
Abdulmaged M. Traish ◽  
Ahmad Haider ◽  
Karim Sultan Haider ◽  
Gheorghe Doros ◽  
Farid Saad
Keyword(s):  
Repeated Measures ◽  
Fixed Effects ◽  
Random Effect ◽  
Treated Group ◽  
Myocardial Infarctions ◽  
Control Group ◽  
Cv Disease ◽  
High Level ◽  
Changes Over Time

Objectives: In the absence of large, prospective, placebo-controlled studies of longer duration, substantial evidence regarding the safety and risk of testosterone (T) therapy (TTh) with regard to cardiovascular (CV) outcomes can only be gleaned from observational studies. To date, there are limited studies comparing the effects of long-term TTh in men with hypogonadism who were treated or remained untreated with T, for obvious reasons. We have established a registry to assess the long-term effectiveness and safety of T in men in a urological setting. Here, we sought to compare the effects of T on a host of parameters considered to contribute to CV risk in treated and untreated men with hypogonadism (control group). Patients and Methods: Observational, prospective, cumulative registry study in 656 men (age: 60.7 ± 7.2 years) with total T levels ≤12.1 nmol/L and symptoms of hypogonadism. In the treatment group, men (n = 360) received parenteral T undecanoate (TU) 1000 mg/12 weeks following an initial 6-week interval for up to 10 years. Men (n = 296) who had opted against TTh served as controls. Median follow-up in both groups was 7 years. Measurements were taken at least twice a year, and 8-year data were analyzed. Mean changes over time between the 2 groups were compared by means of a mixed-effects model for repeated measures, with a random effect for intercept and fixed effects for time, group, and their interaction. To account for baseline differences between the 2 groups, changes were adjusted for age, weight, waist circumference, fasting glucose, blood pressure, and lipids. Results: There were 2 deaths in the T-treated group, none was related to CV events. There were 21 deaths in the untreated (control) group, 19 of which were related to CV events. The incidence of death in 10 patient-years was 0.1145 in the control group (95% confidence interval [CI]: 0.0746-0.1756; P < .000) and 0.0092 in the T-treated group (95% CI: 0.0023-0.0368; P < .000); the estimated difference between groups was 0.0804 (95% CI: 0.0189-0.3431; P < .001). The estimated reduction in mortality for the T-group was between 66% and 92%. There were also 30 nonfatal strokes and 26 nonfatal myocardial infarctions in the control group and none in the T-treated group. Conclusion: Long-term TU was well tolerated with excellent adherence suggesting a high level of patient satisfaction. Mortality related to CV disease was significantly reduced in the T-group.

Abstract TP85: The Kca3.1 Tram-34 Reduces Selective Neuronal Loss, Microglial Activation and Sensorimotor Deficits Following Brief Middle-cerebral Artery Occlusion (mcao) in the Rat

Stroke ◽  
2017 ◽  
Vol 48 (suppl_1) ◽  
Author(s):  
Jean-Claude Baron ◽  
Julius Emmrich ◽  
Sohail Ejaz ◽  
David J Williamson
Keyword(s):  
Repeated Measures ◽  
Microglial Activation ◽  
Spontaneously Hypertensive Rat ◽  
Cell Damage ◽  
Neuronal Loss ◽  
Treated Group ◽  
Artery Occlusion ◽  
Motor Deficit ◽  
Control Group ◽  
Significant Group

Introduction: We have previously shown that 15min distal MCAo in the spontaneously hypertensive rat (SHR) consistently induces isolated selective neuronal loss (SNL) and topographically congruent microglial activation (MA), together with slowly recovering sensori-motor deficit (SMD) (Ejaz et al, Stroke , 2015). Whether MA exacerbates SNL in the reperfused penumbra is unclear. We tested whether reducing MA by means of the KCa3.1 blocker TRAM-34 also impacts SNL and SMD in this model. Methods: 15min microclip distal MCAo was performed in 12 isoflurane-anesthetized adult male SHRs, randomized in two groups of n=6 rats, a control group and a treated group that received TRAM-34 40mg/kg i.p. bd for 1 week starting 12hrs post-MCAo, then od for 3 weeks. To quantify SMD, the sticky label test was administered 3 times a week for 28 days. NeuN and Iba4 immunoflorescence was then performed. Manual delineation of areas of SNL and MA was carried out by two independent assessors on randomly presented IF sections, and volumes of SNL and MA were determined. Both assessments were done blinded to subject’s group. Results: Although less so than in controls, SMD was significant in the TRAM-34 group (p<.001 and .01, respectively), with a significant Group x Time interaction (p=0.004, repeated-measures ANOVA) and a trend for faster recovery from day 7 in the latter. On IF, the volume (mm 3 ) of MA was lower in the TRAM-34 relative to control group (1.33 ± 1.7 vs 0.27 ± 0.46), which also applied to SNL (1.41 ± 1.79 vs 0.28 ± 0.41) (p = 0.018 and 0.015, respectively; two-sample t-test). Conclusion: In this pilot study, the expected marked reduction in MA induced by TRAM34 was associated with concomitant reductions in SNL and faster SMD recovery. Thus, the early administration of an MA blocker following treatment-induced reperfusion in the clinical setting might reduce cell damage in the salvaged penumbra and in turn enhance neurological recovery.

The predatory capability of three nematophagous fungi in the control of Haemonchus contortus infective larvae in ovine faeces

2003 ◽  
Vol 77 (4) ◽  
pp. 297-303 ◽  
Author(s):  
J. Flores-Crespo ◽  
D. Herrera-Rodríguez ◽  
P. Mendoza de Gives ◽  
E. Liébano-Hernández ◽  
V.M. Vázquez-Prats ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Biological Control Agent ◽  
Aqueous Suspension ◽  
Three Dimensional ◽  
Control Agent ◽  
Duddingtonia Flagrans ◽  
Arthrobotrys Oligospora ◽  
Larval Population ◽  
Similar Treatment ◽  
Infective Larvae

AbstractThe effect of oral administration of three different nematode-trapping fungi, in aqueous suspension containing either Dactylaria sp. or Arthrobotrys oligospora conidia or Duddingtonia flagrans chlamydospores, on the number of Haemonchus contortus infective larvae in sheep faeces, was evaluated. The three selected species of fungi produce three-dimensional adhesive nets in the presence of nematodes. Sixteen Creole sheep were divided into four groups of four animals each. Groups 1 and 2 were orally drenched with a suspension containing 2×107 conidia of either A. oligospora or Dactylaria sp. Group 3, received a similar treatment, with D. flagrans chlamydospores, instead of conidia, being administered, at the same dose. Group 4 acted as control, without any fungi. Faecal samples were collected directly from the rectum of each sheep and faecal cultures were prepared and incubated at 15 and 21 days. Larvae were recovered from faecal cultures and counted. The highest reduction of the nematode population occurred in the D. flagrans group, reaching reductions of 96.3% and 91.4% in individual samplings in plates incubated for 15 and 21 days, respectively. Arthrobotrys oligospora showed moderate reductions in the faecal larval population, ranging between 25–64% at 15 days incubation. In general, Dactylaria sp., was less efficient in its trapping ability. Despite the inconsistent results with Dactylaria sp., reduction percentages of 73.4% and 80.7% were recorded in individual samplings during the first and second days, in plates incubated for 15 days. Duddingtonia flagrans, was shown to be a potential biological control agent of H. contortus infective larvae.

scholarly journals Effects of Lactobacillus acidophilus D2/CSL (CECT 4529) supplementation on healthy cat performance

2019 ◽  
Vol 6 (1) ◽  
pp. e000368 ◽  
Author(s):  
Eleonora Fusi ◽  
Rita Rizzi ◽  
Michele Polli ◽  
Simona Cannas ◽  
Alberto Giardini ◽  
...  
Keyword(s):  
Body Condition Score ◽  
Probiotic Strain ◽  
Experimental Period ◽  
Treated Group ◽  
Quality Parameters ◽  
Control Group ◽  
Gut Health ◽  
Positive Effects ◽  
Condition Score ◽  
Dry Diet

BackgroundThe present study aimed to evaluate the effects of the probiotic strain Lactobacillusacidophilus D2/CSL (CECT 4529) on nutritional condition and faecal quality in cats.MethodsTen healthy adult cats from the same cattery were included (aged >9 months; male:female sex ratio=3:7). The animals were randomly assigned to a control group (CTR; n=5; male:female=1:4; room 1: 16 m2) and to a treated group (LACTO; n=5; male:female=2:3; room 2: 16 m2) receiving the same commercial dry diet. The LACTO group diet was supplemented with the probiotic (5 x 109 cfu/kg feed at least). A five-week experimental period was applied, and nutritional status was monitored by bodyweight (BW) and body condition score (BCS). Faecal quality was evaluated using faecal score (FS) and faecal moisture (FM) parameters. Plate counts of some faecal bacteria species were carried out. The data obtained were analysed using MIXED, GLM and NPAR1WAY procedures (SAS V.9.4; P≤0.05).ResultsThe two groups did not show differences in BW and BCS data. A clear effect of the probiotic supplementation on FM was recorded (LACTO 44 per cent v CTR group 46 per cent; P=0.04). FS in the LACTO group (3.35) was close to ideal values (2–3) in comparison with the CTR group (3.75). Positive effects of Lacidophilus D2/CSL have been recorded in terms of increase in faecal lactobacilli counts and reduction in faecal coli counts.ConclusionsThis study’s preliminary results describe how inclusion of Lacidophilus D2/CSL (CECT 4529) probiotic strain in cats’ diets could effectively improve faecal quality parameters and consequently gut health in adult healthy cats.

Evaluating the effectiveness of a Mexican strain ofDuddingtonia flagransas a biological control agent against gastrointestinal nematodes in goat faeces

2005 ◽  
Vol 79 (2) ◽  
pp. 151-157 ◽  
Author(s):  
N.F. Ojeda-Robertos ◽  
P. Mendoza-de Gives ◽  
J.F.J. Torres-Acosta ◽  
R.I. Rodríguez-Vivas ◽  
A.J. Aguilar-Caballero
Keyword(s):  
Single Dose ◽  
Live Weight ◽  
Gastrointestinal Nematode ◽  
Treated Group ◽  
Control Group ◽  
Post Inoculation ◽  
Duddingtonia Flagrans ◽  
Infective Larvae ◽  
Nematode Larvae ◽  
Petri Dishes

AbstractThe use ofDuddingtonia flagransin the control of goat nematodes was investigated. Initially, the time of passage of chlamydospores through the digestive tract of goats was evaluated. Two groups of seven parasite-free kids were formed. Group A received a single dose of 3.5×106D. flagranschlamydospores (FTHO-8 strain) per kg of live weight. Group B did not receive any chlamydospores. Faeces were obtained from each kid daily from day 4 prior to inoculation until day 5 post-inoculation (PI) and were placed in Petri dishes containing water agar. Gastrointestinal nematode infective larvae were added to each Petri dish and incubated at 25°C for 7 days. Petri dishes were examined to detect the fungus and trapped nematodes. A second trial evaluated the effect ofD. flagranson the number of gastrointestinal nematode larvae harvested from goat faecal cultures in naturally infected goats. Two groups of seven goats were formed. The treated group received a single dose of 3.5×106D. flagranschlamydospores per kg of liveweight. The control group did not receive any chlamydospores. Faeces were obtained twice daily from each kid. Two faecal cultures were made for each kid. One was incubated for 7 days and the other for 14 days. Gastrointestinal nematode larvae were recovered from each culture and counted. Percentage of larval development reduction was determined using a ratio of larvae/eggs deposited in the control and treated groups.Duddingtonia flagranssurvived the digestive process of goats, and maintained its predatory activity, being observed from 21 to 81 h PI (3 to 4 days). A reduction in the infective larvae population in the treated group compared to the non-treated group was observed in both incubation periods (7 days: 5.3–36.0%; 14 days: 0–52.8%,P>0.05). Although a single inoculation ofD. flagranscan induce a reduction of infective larvae collected from faeces, a different scheme of dosing may be needed to enhance the efficacy ofD. flagransin goats.

scholarly journals Use of a glycogenic precursor during the prepartum period and its effects upon metabolic indicators and reproductive parameters in dairy cows

2020 ◽  
Vol 11 (2) ◽  
pp. 408-420
Author(s):  
Carlos Leyva Orasma ◽  
Jesús Jaime Benitez-Rivas ◽  
Juan Luis Morales Cruz ◽  
Cesar Alberto Meza-Herrera ◽  
Oscar Ángel-García ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Transition Period ◽  
Body Condition Score ◽  
Treated Group ◽  
Control Group ◽  
Blood Levels ◽  
Early Lactation ◽  
Homogeneous Groups ◽  
Subclinical Ketosis ◽  
Retained Placenta

The aim was to evaluate if 1-2 propanodiol plus calcium propionate (glycogenic precursor) supplementation during the transition period in high yielding dairy cows reduces metabolic and reproductive dysfunctions during early lactation. Cows (n=202) where divided into two homogeneous groups regarding number of lactations and body condition score. 1) Treated Group (GG; n=112) received 60g/cow/day  15d of a glycogenic precursor during the transition period. 2) Control Group (GC; n = 90) received no treatment. Postpartum levels of BHB (GG= 0.9±0.2 mmol/L vs GC =1.3±0.2 mmol/L; P < 0.05), and NEFA (GG= 0.6 ± 0.1 mEq/L vs GC = 0.8 ± 0.1 mEq/L; P < 0.05) were higher for GC. Likewise, GC-cows had a higher percentage of retained placenta (23% vs. 13%; P ≤ 0.06) subclinical ketosis (GG = 10%, GC = 56%; P < 0.05), and mastitis (GG = 8%, GC = 16%; P < 0.05). Metritis, dystocia, abortions, clinical ketosis, hypocalcemia and ruminal acidosis showed no differences. Administration of a glycogenic precursor during the transition period demonstrated a positive effect upon BHB and NEFA blood levels during early lactation. Also, levels of subclinical ketosis and retained placenta were reduced.

scholarly journals Activity in vitro of fungal conidia of Duddingtonia flagrans and Monacrosporium thaumasium on Haemonchus contortus infective larvae

2010 ◽  
Vol 85 (2) ◽  
pp. 138-141 ◽  
Author(s):  
A.R. Silva ◽  
J.V. Araújo ◽  
F.R. Braga ◽  
C.D.F. Alves ◽  
L.N. Frassy
Keyword(s):  
Haemonchus Contortus ◽  
Control Group ◽  
Duddingtonia Flagrans ◽  
Control Groups ◽  
Infective Larvae ◽  
Fungal Isolates ◽  
Petri Dishes ◽  
And Control ◽  
Baermann Method

AbstractThe objective of this work was to evaluate the predatory activity of the fungi Duddingtonia flagrans (AC001) and Monacrosporium thaumasium (NF34a) on Haemonchus contortus infective larvae (L3) in two experimental assays (A and B). In assay A, two treatments and one control were formed and kept for 7 days in Petri dishes with 2% water-agar. Each treatment consisted of 1000 H. contortus L3 and 1000 conidia of only one fungal isolate, and the control group consisted of 1000 L3, without fungus, with 10 repetitions per group. In assay B, 1000 conidia of one of the fungal isolates, AC001 or NF34a, were added to coprocultures made from 20 g of faeces collected from sheep naturally infected with H. contortus. At the end of the experiment, the Baermann method was used to count the non-predated larvae of all Petri dishes from treatment and control groups. In assay A, no difference was observed (P>0.05) between the groups treated with AC001 and NF34a fungi. A difference was observed (P < 0.05) between the treated and control groups. The L3 reduction percentages at the end of the experiment were 87.75 and 85.57%, respectively, for the fungal isolates compared to the control group. In assay B, the reduction percentages for conidia of these isolates were 85.82 and 87.32%, respectively. The results obtained show that D. flagrans (AC001) and M. thaumasium (NF34a) were effective in the in vitro control of sheep H. contortus L3 and could be used in the biological control of this nematode.

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