COI sequencing as tool for the taxonomic attribution ofPatellaspp. (Gastropoda): the case of morphologically undistinguishable juveniles settled on aPatella ferrugineaadult

2010 ◽  
Vol 90 (7) ◽  
pp. 1449-1454 ◽  
Author(s):  
Marco Casu ◽  
Daria Sanna ◽  
Benedetto Cristo ◽  
Tiziana Lai ◽  
Gian Luca Dedola ◽  
...  
Keyword(s):  
Cluster Analysis ◽  
Maximum Likelihood ◽  
Genetic Analysis ◽  
Dna Barcoding ◽  
Western Mediterranean ◽  
The Other ◽  
Assignment Test ◽  
Test Results ◽  
Molecular Approach ◽  
Patella Ferruginea

Patella ferrugineais an endangered marine gastropod, distributed on the western Mediterranean coasts, whose range has progressively contracted, due to intense human exploitation. A genetic analysis was performed on two unidentified young individuals belonging to the genusPatellafound attached to the shell of an adult ofP. ferruginea, with a twofold aim: (i) to achieve their correct taxonomic attribution by means of the DNA barcoding; and (ii) to shed some light on the hypothesized larval philopatry and/or juveniles phoresis inP. ferruginea. The survey was carried out comparing the sequences of the cytochrome c oxidase subunit I (COI) Folmer region obtained for the two juveniles with those obtained for adults ofP. ferruginea, P. caerulea, P. rustica, andP. ulyssiponensis, from different sites of the western Mediterranean, by means of maximum likelihood cluster analysis and a Bayesian-based assignment test. Results obtained evidenced that: (i) COI may be used with confidence as DNA barcoding in the genusPatella; and (ii) the two juveniles studied are not conspecific: one belonged toP. ferruginea, the other toP. rustica.The latter finding raises doubts about the juvenile phoresis and about the occurrence of larval philopatry inP. ferruginea, suggesting that an extensive use of a molecular approach for a better evaluation of the recruitment features of this endangered species should be adopted.

Broccoli ‘Fiolaro’ (Brassica oleracea) an endangered typical Italian cultivar. A genetic analysis by SSR markers

2008 ◽  
Vol 6 (3) ◽  
pp. 215-221 ◽  
Author(s):  
M. Vischi ◽  
M. Fiori ◽  
E. De Paoli ◽  
S. Padovan ◽  
M. Guarda ◽  
...  
Keyword(s):  
Cluster Analysis ◽  
Brassica Oleracea ◽  
Cultivar Identification ◽  
The Other ◽  
Assignment Test ◽  
Significant Differentiation ◽  
Hill Country ◽  
North Eastern ◽  
Degree Of Similarity ◽  
Simple Sequence

‘Broccolo fiolaro’ (Brassica oleracea L. convar. Italica) is a typical vegetable produced in a restricted hill country area around Creazzo (Vicenza) in north-eastern Italy. The cultivation of this vegetable dates back several centuries ago, but a very few farms are still involved in the production. ‘Broccolo fiolaro’ is a variety of broccoli highly valued for its agronomic and organoleptic features.Four ‘Broccolo fiolaro’ selections were characterized by simple sequence repeats (SSRs) molecular markers and compared with other five cultivated varieties of broccoli of the Italica group in order to reconstruct their genetic structure and evaluate the degree of similarity among and within populations. The analysis of 12 SSR polymorphic loci pointed out a low genetic variability among the four ‘Broccolo fiolaro’ selections. Moreover, the whole ‘Fiolaro’ group showed significant differentiation from the other broccoli cultivars and could be easily distinguished by cluster analysis. An assignment test on ~30 genotyped plants randomly chosen from each broccoli population correctly recognized the proper accession 88% of the time, proving this method to be useful for cultivar identification.

DNA barcoding and morphology support the division of Elachista nuraghella sensu auctorum (Lepidoptera: Elachistidae: Elachistinae) into two vicariant species

Zootaxa ◽  
2012 ◽  
Vol 3343 (1) ◽  
pp. 57 ◽  
Author(s):  
LAURI KAILA ◽  
MARKO MUTANEN
Keyword(s):  
Distribution Pattern ◽  
Dna Barcoding ◽  
Mediterranean Region ◽  
Western Mediterranean ◽  
The Other ◽  
Female Genitalia ◽  
Male And Female

Elachista nuraghella sensu auct. (Lepidoptera, Elachistidae) is shown to display a striking division of haplotype groups in theDNA barcode sequence, one widely distributed in the western Mediterranean region, the other in the east: Bulgaria, Greece andTurkey. The haplotypes correspond with constant differences in both male and female genitalia, and generally also in outerappearance. E. nuraghella Amsel is thus considered to consist of two species displaying a vicariant distribution pattern. The eastern taxon is described as Elachista grotenfelti Kaila sp. nov.

Adding an expansive agent to increase the toughness of steel fibre reinforced concrete

2019 ◽  
Vol 26 (4) ◽  
pp. 197-208
Author(s):  
Leo Gu Li ◽  
Albert Kwok Hung Kwan
Keyword(s):  
Reinforced Concrete ◽  
Steel Fibre ◽  
The Other ◽  
Fibre Reinforced Concrete ◽  
Test Results ◽  
Compressive Properties ◽  
Shrinkage Cracking ◽  
Steel Fibre Reinforced Concrete ◽  
Expansive Agent ◽  
Concrete Matrix

Previous research studies have indicated that using fibres to improve crack resistance and applying expansive agent (EA) to compensate shrinkage are both effective methods to mitigate shrinkage cracking of concrete, and the additions of both fibres and EA can enhance the other performance attributes of concrete. In this study, an EA was added to fibre reinforced concrete (FRC) to produce concrete mixes with various water/binder (W/B) ratios, steel fibre (SF) contents and EA contents for testing of their workability and compressive properties. The test results showed that adding EA would slightly increase the superplasticiser (SP) demand and decrease the compressive strength, Young’s modulus and Poisson’s ratio, but significantly improve the toughness and specific toughness of the steel FRC produced. Such improvement in toughness may be attributed to the pre-stress of the concrete matrix and the confinement effect of the SFs due to the expansion of the concrete and the restraint of the SFs against such expansion.

scholarly journals Evaluating Presence/Absence of Target Microbes in Microbiological Tests

2000 ◽  
Vol 83 (6) ◽  
pp. 1429-1434
Author(s):  
Robert J Blodgett ◽  
Anthony D Hitchins
Keyword(s):  
Performance Studies ◽  
Collaborative Study ◽  
False Negative ◽  
The Other ◽  
Test Results ◽  
Logistic Curve ◽  
Microbiological Method ◽  
Test Portion ◽  
Method Performance ◽  
Data Set

Abstract A typical qualitative microbiological method performance (collaborative) study gathers a data set of responses about a test for the presence or absence of a target microbe. We developed 2 models that estimate false-positive and false-negative rates. One model assumes a constant probability that the tests will indicate the target microbe is present for any positive concentration in the test portion. The other model assumes that this probability follows a logistic curve. Test results from several method performance studies illustrate these estimates.

scholarly journals Effect of Fly Ash on Compressive Strength, Drying Shrinkage, and Carbonation Depth of Mortar with Ferronickel-Slag Powder

2021 ◽  
Vol 11 (3) ◽  
pp. 1037
Author(s):  
Se-Jin Choi ◽  
Ji-Hwan Kim ◽  
Sung-Ho Bae ◽  
Tae-Gue Oh
Keyword(s):  
Compressive Strength ◽  
Fly Ash ◽  
Construction Industry ◽  
Bituminous Coal ◽  
Raw Materials ◽  
Drying Shrinkage ◽  
The Other ◽  
Test Results ◽  
Slag Powder ◽  
Ferronickel Slag

In recent years, efforts to reduce greenhouse gas emissions have continued worldwide. In the construction industry, a large amount of CO2 is generated during the production of Portland cement, and various studies are being conducted to reduce the amount of cement and enable the use of cement substitutes. Ferronickel slag is a by-product generated by melting materials such as nickel ore and bituminous coal, which are used as raw materials to produce ferronickel at high temperatures. In this study, we investigated the fluidity, microhydration heat, compressive strength, drying shrinkage, and carbonation characteristics of a ternary cement mortar including ferronickel-slag powder and fly ash. According to the test results, the microhydration heat of the FA20FN00 sample was slightly higher than that of the FA00FN20 sample. The 28-day compressive strength of the FA20FN00 mix was approximately 39.6 MPa, which was higher than that of the other samples, whereas the compressive strength of the FA05FN15 mix including 15% of ferronickel-slag powder was approximately 11.6% lower than that of the FA20FN00 mix. The drying shrinkage of the FA20FN00 sample without ferronickel-slag powder was the highest after 56 days, whereas the FA00FN20 sample without fly ash showed the lowest shrinkage compared to the other mixes.

scholarly journals Generation of an EDS Key Based on a Graphic Image of a Subject’s Face Using the RC4 Algorithm

Information ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 19
Author(s):  
Alexey Semenkov ◽  
Dmitry Bragin ◽  
Yakov Usoltsev ◽  
Anton Konev ◽  
Evgeny Kostuchenko
Keyword(s):  
Image Recognition ◽  
Random Number ◽  
Statistical Test ◽  
The Other ◽  
Test Results ◽  
Random Generation ◽  
Facial Image ◽  
Random Number Generators ◽  
Graphic Image ◽  
High Level

Modern facial recognition algorithms make it possible to identify system users by their appearance with a high level of accuracy. In such cases, an image of the user’s face is converted to parameters that later are used in a recognition process. On the other hand, the obtained parameters can be used as data for pseudo-random number generators. However, the closeness of the sequence generated by such a generator to a truly random one is questionable. This paper proposes a system which is able to authenticate users by their face, and generate pseudo-random values based on the facial image that will later serve to generate an encryption key. The generator of a random value was tested with the NIST Statistical Test Suite. The subsystem of image recognition was also tested under various conditions of taking the image. The test results of the random value generator show a satisfactory level of randomness, i.e., an average of 0.47 random generation (NIST test), with 95% accuracy of the system as a whole.

scholarly journals Genetics of Differences in Pheromonal Hydrocarbons Between Drosophila melanogaster and D. simulans

Genetics ◽  
1996 ◽  
Vol 143 (1) ◽  
pp. 353-364 ◽  
Author(s):  
Jerry A Coyne
Keyword(s):  
Drosophila Melanogaster ◽  
Sexual Dimorphism ◽  
Genetic Analysis ◽  
Species Difference ◽  
The Other ◽  
Chromosome 3 ◽  
Apparent Effect ◽  
The Third ◽  
Hydrocarbon Profile ◽  
The Right

Abstract Females of Drosophila melanogaster and its sibling species D. simulans have very different cuticular hydrocarbons, with the former bearing predominantly 7,11-heptacosadiene and the latter 7-tricosene. This difference contributes to reproductive isolation between the species. Genetic analysis shows that this difference maps to only the third chromosome, with the other three chromosomes having no apparent effect. The D. simulans alleles on the left arm of chromosome 3 are largely recessive, allowing us to search for the relevant regions using D. melanogaster deficiencies. At least four nonoverlapping regions of this arm have large effects on the hydrocarbon profile, implying that several genes on this arm are responsible for the species difference. Because the right arm of chromosome 3 also affects the hydrocarbon profile, a minimum of five genes appear to be involved. The large effect of the third chromosome on hydrocarbons has also been reported in the hybridization between D. simulans and its closer relative D. sechellia, implying either an evolutionaly convergence or the retention in D. sechllia of an ancestral sexual dimorphism.

scholarly journals Standardization of a method to study angiogenesis in a mouse model

2013 ◽  
Vol 85 (4) ◽  
pp. 1483-1487
Author(s):  
DAVID FEDER ◽  
FABIO F. PERRAZO ◽  
EDIMAR C. PEREIRA ◽  
SILVANA FORSAIT ◽  
CECILIA K.R. FEDER ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Animal Models ◽  
The Other ◽  
In Vivo Studies ◽  
Specific Method ◽  
Test Results ◽  
Hemoglobin Content ◽  
Physiological Conditions ◽  
Adult Organism

In the adult organism, angiogenesis is restricted to a few physiological conditions. On the other hand, uncontrolled angiogenesis have often been associated to angiogenesis-dependent pathologies. A variety of animal models have been described to provide more quantitative analysis of in vivo angiogenesis and to characterize pro- and antiangiogenic molecules. However, it is still necessary to establish a quantitative, reproducible and specific method for studies of angiogenesis factors and inhibitors. This work aimed to standardize a method for the study of angiogenesis and to investigate the effects of thalidomide on angiogenesis. Sponges of 0.5 x 0.5 x 0.5 cm were implanted in the back of mice groups, control and experimental (thalidomide 200 mg/K/day by gavage). After seven days, the sponges were removed. The dosage of hemoglobin in sponge and in circulation was performed and the ratio between the values was tested using nonparametric Mann-Whitney test. Results have shown that sponge-induced angiogenesis quantitated by ratio between hemoglobin content in serum and in sponge is a helpful model for in vivo studies on angiogenesis. Moreover, it was observed that sponge-induced angiogenesis can be suppressed by thalidomide, corroborating to the validity of the standardized method.

scholarly journals First record of Solea (Microchirus) boscanion (Osteichthyes: Soleidae) in the Mediterranean Sea, with data on other sympatric soleid species

2002 ◽  
Vol 82 (5) ◽  
pp. 907-911 ◽  
Author(s):  
Enric Massutí ◽  
J.A. Reina-Hervás ◽  
Domingo Lloris ◽  
L. Gil de Sola
Keyword(s):  
Climate Change ◽  
Mediterranean Sea ◽  
First Record ◽  
Western Mediterranean ◽  
The Other ◽  
The Mediterranean

The capture of five specimens of Solea (Microchirus) boscanion (Osteichthyes: Soleidae), a species previously unrecorded in the Mediterranean, is reported from the Iberian coast (western Mediterranean). The main morphometric and meristic measurements of this species with data of the other sympatric, and morphologically very similar, soleids Microchirus variegatus and Buglossidium luteum are also given. The record is discussed in relation to climate change and competition between species.

scholarly journals Tracers confirm downward mixing of Tyrrhenian Sea upper waters associated with the Eastern Mediterranean Transient

2010 ◽  
Vol 7 (4) ◽  
pp. 1533-1557
Author(s):  
W. Roether ◽  
J. E. Lupton
Keyword(s):  
Deep Water ◽  
Time Scale ◽  
Eastern Mediterranean ◽  
Western Mediterranean ◽  
The Other ◽  
Tyrrhenian Sea ◽  
Convective Mixing ◽  
Deep Waters ◽  
Major Process ◽  
Gibraltar Strait

Abstract. Observations of tritium and 3He in the Tyrrhenian Sea, 1987–2009, confirm the enhanced convective mixing of intermediate waters into the deep waters that has been noted and associated with the Eastern Mediterranean Transient in previous studies. Our evidence for the mixing rests on increasing tracer concentrations in the Tyrrhenian deep waters, accompanied by decreases in the upper waters, which are supplied from the Eastern Mediterranean. The downward transfer is particularly evident between 1987 and 1997. Later on, information partly rests on increasing tritium-3He ages; here we correct the observed 3He for contributions released from the ocean floor. The Tyrrhenian tracer distributions are fully compatible with data upstream of the Sicily Strait and in the Western Mediterranean. The tracer data show that mixing reached to the bottom and confirm a cyclonic nature of the deep water circulation in the Tyrrhenian. They furthermore indicate that horizontal homogenization of the deep waters occurs on a time scale of several years. Various features point to a reduced impact of Western Mediterranean Deep Water (WMDW) in the Tyrrhenian during the enhanced-convection period. This is an important finding because it implies less upward mixing of WMDW, which has been named a major process to enable the WMDW to leave the Mediterranean via the Gibraltar Strait. On the other hand, the TDW outflow for several years represented a major influx of enhanced salinity and density waters into the deep-water range of the Western Mediterranean.

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