Optimum dietary amino acid pattern and limiting order of some essential amino acids for growing-furring blue foxes (Alopex lagopus)

2004 ◽  
Vol 78 (1) ◽  
pp. 77-86 ◽  
Author(s):  
T. Dahlman ◽  
J. Valaja ◽  
E. Venäläinen ◽  
T. Jalava ◽  
I. Pölönen
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Control Diet ◽  
Essential Amino Acids ◽  
Amino Acid Pattern ◽  
N Retention ◽  
Low Protein ◽  
Acid Control ◽  
Blue Fox ◽  
Limiting Amino Acid

AbstractThe optimum pattern and limiting order of some essential amino acids for growing-furring blue foxes were assessed from nitrogen (N) retention responses. Total tract digestibility and N balance trials were carried out on 24 weaned blue fox males in an 8 ✕ 5 cyclic change-over experiment. Eight experimental diets were prepared by removing proportionately about 0·4 of each of the amino acids studied – methionine + cystine, lysine, threonine, tryptophan and histidine – successively from the amino acid control diet. The main source of protein in the amino acid control diet was casein and an amino acid mixture was added to bring the calculated crude protein (CP) content up to the level of 170 g/kg dry matter (DM). Low-protein (CP 95·7 g/kg DM) and high-protein (CP 166·6 g/kg DM) diets, the protein proportion of which was casein protein, served as negative and positive control diets, respectively. The reduction in N retention when one amino acid in turn was deleted from the amino acid control diet was calculated, and a regression analysis was made between N retention and relative amino acid intake. Data on the animals’ intake of each limiting amino acid and those on the amino acid control diet were used. The optimum amino acid pattern, expressed relative to lysine = 100, proved to be: methionine + cystine 77, threonine 64, histidine 55 and tryptophan 22. The first-limiting amino acids were methionine + cystine. Blue fox responses (N retention, weight gain) to deletion of methionine + cystine from the diet were very severe and exceeded those to deletion of any other amino acid. Moreover, removing methionine + cystine from the diet significantly impaired the apparent digestibility of organic matter, reducing it to a level even lower than that of the low-protein diet. After methionine + cystine, the next-limiting amino acid in casein-based diets was threonine, followed by histidine and tryptophan. The results show the importance of verifying the sufficiency of dietary methionine + cystine in the practical feeding of blue foxes.

An optimal dietary amino acid pattern for growing pigs

1997 ◽  
Vol 1987 ◽  
pp. 91-91 ◽  
Author(s):  
T. C. Wang ◽  
M. F. Fuller
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Nitrogen Retention ◽  
Essential Amino Acids ◽  
Growing Pigs ◽  
Amino Acid Pattern ◽  
Dietary Amino Acid ◽  
Ideal Protein ◽  
Limiting Amino Acid ◽  
The Ideal

An animal's rate of protein accretion (or nitrogen retention) is determined by the most deficient (or first limiting) amino acid in the diet, in a perfectly balanced (or ideal) protein all essential amino acids and the sum of the non-essential amino acids are equally limiting. If a dietary protein had any amino acids in excess of the ideal pattern, then the removal of any of the excess should not affect nitrogen retention. This principle was used to determine the ideal dietary amino acid pattern for growing pigs.Three nitrogen balance trials were carried out with a total of 64 gilts of weight from 30 to 55 kg. Casein and a mixture of amino acids were used in the semi-purified diets. The animals were given the diets at the rate of 93 g/kg BW0.75/d in three equal meals mixed with 0.3 L water. The feeding times were 08.30, 12.30 and 17.30. All pigs received their experimental diets for 7 days, made up of 3 days preliminary and a 4-day collection, except that the preliminary period before the first collection in the first period was 7 days. Before the first collection bladder catheters were introduced.

scholarly journals The optimum dietary amino acid pattern for growing pigs

1989 ◽  
Vol 62 (1) ◽  
pp. 77-89 ◽  
Author(s):  
T. C. Wang ◽  
M. F. Fuller
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Agricultural Research ◽  
Essential Amino Acids ◽  
Growing Pigs ◽  
Amino Acid Pattern ◽  
Single Amino Acid ◽  
N Retention ◽  
Dietary Amino Acid ◽  
Optimum Balance

A series of four nitrogen-balance experiments was carried out with growing pigs to determine the optimum balance amongst the amino acids in the diet. The reduction in N retention when 20 % of a single amino acid was removed from the diet was used to calculate a dietary amino acid pattern in which each amino acid would he equally limiting. A mixture of amino acids simulating the amino acid pattern of casein was used with the same efficiency as casein. From two successive deletion experiments an optimum balance amongst the essential amino acids was derived. Expressed relative to lysine = 100 this had threonine 72, valine 75, methionine + cystine 63, isoleucine 60, leucine 110, phenylalanine + tryosine 120, tryptophan 18. No estimate was made for histidine. Essential amino acids in this pattern were mixed with non-essential amino acids in ratios of 36:64 up to 57:43. The highest efficiency of N retention was achieved with diets having a ratio of at least 45:55. This included (g/16 g N) lysine 6 5, threonine 4.7, valine 4 9, methionine + cystine 4.1, isoleucine 3 9, leucine 7.2, phenylalanine + tyrosine 7.8, tryptophan 12. The N of diets with this amino acid pattern was utilized significantly better than when the pattern proposed by the Agricultural Research Council (1981) was used. The flow of amino acids past the terminal ileum of pigs given the semi-synthetic diet with this amino acid pattern was no greater than that observed with protein-free diets. The proposed pattern thus describes the intrinsic requirements of the growing pig for absorbed amino acids.

Supplementation of a low protein diet in an attempt to optimize egg mass output

1991 ◽  
Vol 71 (1) ◽  
pp. 211-220 ◽  
Author(s):  
John D. Summers ◽  
J. L. Atkinson ◽  
D. Spratt
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Soybean Meal ◽  
Egg Production ◽  
Corn Oil ◽  
Control Diet ◽  
Essential Amino Acids ◽  
Egg Mass ◽  
Minimum Requirement ◽  
Low Protein

Pullets were brought into production on a low-protein corn, soybean meal diet to which various nutrients including essential amino acids, choline, corn oil and feathermeal were supplemented. Production commenced between 18 and 20 wk of age and up to at least 60% production, hens fed the low protein diets (10% CP) produced at least as many eggs and as great an egg mass as these given a 17% protein corn, soybean meal control diet. Pullets fed the corn, soybean meal test diets, peaked at around 80% as compared to slightly over 90% for the control. Egg production immediately fell for birds fed the test diets to around 70% for most of the test treatments. Body weight remained constant or fell after peak production for birds fed the test diets as compared to a normal increase for birds on the control diet. Egg size increased for the test diets at a rate which was comparable to that of the control birds. In a second experiment, with older hens, supplementation of the 10% protein test diet with methionine, lysine, arginine and tryptophan, resulted in intakes of these amino acids which met NRC minimum requirement levels. However, egg mass output was reduced approximately 11% compared to the 17% protein control diet. While intakes of several essential amino acids fell below requirement levels, the degree that valine was calculated to be deficient in both experiments corresponded closely with the reduction in egg mass output of hens fed the test as compared to the control diet. Key words: Lysine, methionine, egg weight, body weight, hens.

scholarly journals The relative effects of a low-protein-high-carbohydrate diet on the free amino acid composition of liver and muscle

1976 ◽  
Vol 36 (2) ◽  
pp. 219-230
Author(s):  
P. G. Lunn ◽  
R. G. Whitehead ◽  
B. A. Baker
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Acid Concentration ◽  
Free Amino ◽  
Essential Amino Acids ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Total Amino Acid ◽  
Low Protein

1. Free amino acid concentrations in the plasma have been compared with those in liver and quadriceps muscle, in rats fed on diets containing 209 (control) and 31 (low-protein) g protein/kg. The effects of the low-protein diet on diurnal variations in these values were also measured.2. In the plasma, the total amino acid concentration was significantly lower in animals given the low-protein diet, at all times of day except 12.00 hours. In the liver, and to a lesser extent the muscle, total amino acid concentration was maintained.3. In the control animals, diurnal variation in the concentrations of both essential and non-essential amino acids was very similar in plasma, liver and muscle. In animals given the low-protein diet, although the same diurnal pattern was maintained for non-essential amino acids, that occurring among the essential amino acids had virtually disappeared.4. In plasma, the mean 24 h concentration of essential amino acids decreased from 24· mmol/l in control animals to only 1·29 mmol/l in the low-protein-fed animals. Concentrations in muscle and liver were reduced by a similar proportion (from 8·6 to 5·56 μmol/g and from 8·67 to 5·05 μmol/g respectively). Conversely the concentrations of non-essential amino acids in animals given the low-protein diet were increased in plasma (from 1·53 to 2·00 mmol/l), muscle (from 12·5 to 14·3 μmol/g), and liver (from 16·8 to 20·5 μmol/g), muscle showing the lowest increase.5. With the exceptions of lysine, threonine, cystine and tyrosine, the concentrations of all other essential amino acids were reduced more in liver than in muscle. The relationship between this and the failure to maintain plasma albumin concentrations is discussed.

scholarly journals Efficiency of utilization of essential amino acids in growing rats at different levels of intake

1985 ◽  
Vol 54 (2) ◽  
pp. 499-508 ◽  
Author(s):  
J. Heger ◽  
Z. Frydrych
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Essential Amino Acid ◽  
Essential Amino Acids ◽  
N Balance ◽  
Growing Rats ◽  
Dietary Concentration ◽  
Limiting Amino Acid ◽  
Maintenance Requirements ◽  
Different Levels

1. Nitrogen balance was studied in growing male SPF-rats fed on diets in which each essential amino acid was varied from zero to about 120% of optimum requirement. From the balance results, optimum and maintenance requirements were estimated as well as the efficiency of utilization of amino acids for growth and growth + maintenance.2. N balance increased with increasing dietary level of the deficient amino acid; the response gradually diminished as the content of the amino acid approached optimum. At zero level of intake, negative N balance was found for all amino acids except histidine. The highest loss of body N was found in the sulphur-amino-acid-free diet and the lowest one in the lysine-free diet.3. Maximal utilization of essential amino acids for growth was found at dietary levels corresponding to 30–60% of optimum requirement and ranged from about 0.65 to 0.85 except for S amino acids and histidine. The utilization of S amino acids was about 0.55 while that of histidine exceeded 1.0. The utilization of amino acids for growth-tmaintenance was maximal at the lowest levels of intake and gradually decreased as the dietary concentration of the limiting amino acid increased. At dietary levels near optimum the utilization was about 06–07, except for S amino acids where the utilization was less than 0.5.

Effect of diet and infusion of volatile fatty acids into the rumen on the concentration of plasma free amino acids in sheep

1982 ◽  
Vol 48 (3) ◽  
pp. 519-526 ◽  
Author(s):  
J. R. Mercer ◽  
E. L. Miller
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino ◽  
Volatile Fatty Acids ◽  
Plasma Concentrations ◽  
Essential Amino Acids ◽  
Protein Nitrogen ◽  
Post Infusion ◽  
Limiting Amino Acid ◽  
The Mean

1. The effect of supplementing barley diets with urea (U), extracted decorticated groundnut meal (GNM) or Peruvian fish meal (PFM) on plasma free amino acid concentrations in sheep have been examined and the first limiting amino acid has been indicated by measuring the changes in the concentration of the plasma essential amino acids (PEAA) during a rumen infusion of a volatile fatty acid (VFA) mixture.2. Three wethers fitted with rumen and re-entrant duodenal cannulas were given isonitrogenous, isoenergetic diets containing (g/kg dry matter (DM)) U 20, GNM 106 or PFM 78, the crude protein (nitrogen × 6.25) contents being 139, 145 and 148 respectively. The sheep were fed hourly, the mean daily dm intake being 0.634 kg.3. Plasma concentrations of valine, threonine, lysine, isoleucine and leucine were linearly related to their concentrations in duodenal digesta.4. A VFA mixture was infused into the rumen for 6 h to supply (mmol/min) acetate 1.47, propionate 0.22 and n-butyrate 0.27. Blood samples were taken 6 h before, during and 12 h after the end of the infusion.5. The concentration of all PEAA decreased relative to the pre-infusion and post-infusion controls but there were no significant differences between diets.6. The mean decreases in concentration averaged over all three diets showed that the decrease in concentration of methionine (41.5%) was far greater than for any other essential amino acid suggesting that under these conditions methionine was the first limiting amino acid.

scholarly journals Changes in neurotransmitter levels associated with the deficiency of some essential amino acids in the diet

1992 ◽  
Vol 68 (2) ◽  
pp. 409-420 ◽  
Author(s):  
José L. Venero ◽  
Antonio J. Herrera ◽  
Alberto Machado ◽  
Josefina Cano
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Substantia Nigra ◽  
Corpus Striatum ◽  
Essential Amino Acids ◽  
Serotoninergic System ◽  
Main Metabolite ◽  
Isoleucine Leucine ◽  
Low Protein ◽  
The Brain

The contents of dopamine (DA) and serotonin (5-HT) and their metabolites were measured in rat substantia nigra and corpus striatum following dietary changes, including restriction of protein content (low-protein diet; LPD) and the contents of several large neutral amino acids (isoleucine, leucine, methionine, phenylalanine, tryptophan and valine) for 25 d. The LPD produced an increase in the concentration of tyrosine (TYR) in the two regions of the brain studied. This effect was also observed with all amino acid deficiencies studied except for valine in the substantia nigra, tryptophan in the striatum and phenylalanine in both regions. Likewise, the concentration of 5-hydroxyindolacetic acid (5-HIAA), the main metabolite of 5-HT, increased in the substantia nigra but not in the striatum after LPD, as well as with all the amino acid deficiencies studied, with the exception of tryptophan deficiency. In this case there was a dramatic effect on all components of the serotoninergic system, with decreases in the concentration of tryptophan (TRP; precursor), 5-HT and 5-HIAA. This behaviour clearly shows an interrelationship between precursor (TRP) availability and 5-HT synthesis and metabolism. With valine deficiency, dopaminergic and serotoninergic systems demonstrated opposite effects in the substantia nigra and the corpus striatum, and the behaviour of the two monoamines was also opposite within each structure. The significance of these changes is discussed.

scholarly journals Limiting amino acids supplementation in low crude protein diets and their impacts on growth performance and carcass composition in Labeo rohita (rohu) adult fish

2023 ◽  
Vol 83 ◽  
Author(s):  
A. Ayub ◽  
F. Rasool ◽  
N. Khan ◽  
S. N. Qaisrani ◽  
S. Parveen ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Crude Protein ◽  
Control Diet ◽  
Amino Acid Level ◽  
Carcass Composition ◽  
Amino Acid Supplementation ◽  
Acid Supplementation ◽  
Low Protein ◽  
Limiting Amino Acids

Abstract Ninety days study was conducted in hapas installed in earthen ponds. Fish of an average initial weight (220g) were evenly distributed in triplicate groups within fifteen hapas. Five experimental diets labeled as T1 (25% CP and NRC recommended amino acid level) as control diet, T2 (with 2% low protein and 5% amino acid supplementation), T3 (with 2% low protein and 10% amino acid supplementation), T4 (with 4% low protein and 10% amino acid supplementation) and T5 (with 4% low protein and 20% amino acid supplementation) were prepared. Fish were fed with @3% of their body weight twice a day at 10.00 & 16:00 hour. Significantly higher percent weight gain (420.18 ± 66.84a) and specific growth rate (13499.33±1273.54a) along with improved feed conversion ratio (1.29 ± 0.09b) and hundred percent survivals were recorded during the trial. Furthermore proximate analysis of meat showed significant improvement in the crude protein level (81.77 ± 0.19a) served with diet containing 20% limiting amino acids mixture. Therefore, limiting amino acids can be a source of cost effective feed and use safely in L. rohita diet.

scholarly journals Chemical composition of wonderful kola (Bucchlozia coriacea) and breadfruit (Artocarpus altilis) seeds grown in south–south, Nigeria

2018 ◽  
Vol 53 (2) ◽  
pp. 125-132
Author(s):  
MO Aremu ◽  
DB Passali ◽  
H Ibrahim ◽  
RO Akinyeye
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Crude Protein ◽  
Analytical Techniques ◽  
Essential Amino Acids ◽  
Amino Acid Profile ◽  
Metabolizable Energy ◽  
Crude Fibre ◽  
Artocarpus Altilis ◽  
Limiting Amino Acid

The proximate and amino acid compositions of Artocarpus altilis and Bucchholzia coriacea were investigated using standard analytical techniques. The respective proximate composition (%) for the Artocarpus altilis and Bucchholzia coriacea samples were: Moisture (5.91 and 5.67); ash (4.39 and 5.13); crude fat (4.42 and 2.76); crude protein (12.27 and 14.78); crude fibre (7.10 and 6.27); carbohydrate (65.91 and 65.39). The calculated fatty acids and metabolizable energy for the Artocarpus altilis and Bucchholzia coriacea samples were 3.54 and 2.21 %; 1492.60 and 1465.01 kJ/100 g, respectively. The most abundant minerals in Artocarpus altilis and Bucchholzia were potassium (658.42 and 369.64 mg/100 g) and phosphorus (382.55 and 261.38 mg/100 g), respectively. The amino acid profile revealed that Artocarpus altilis and Bucchholzia coriacea samples contained nutritionally useful quantities of most of the essential amino acids. The total essential amino acids (TEAA) (with His) were 31.14 and 34.40 g/100 g crude protein for the Artocarpus altilis and Bucchholzia coriacea samples, respectively. The first limiting amino acid was Met + Cys (TSAA) for all the samples and calculated isoeletric points (ρl) were 3.52 and 4.05 for Artocarpus altilis and Bucchholzia coriacea, respectively.Bangladesh J. Sci. Ind. Res.53(2), 125-132, 2018

Effect of dietary limiting amino acid in prepyriform cortex on meal patterns

1990 ◽  
Vol 259 (4) ◽  
pp. R716-R723 ◽  
Author(s):  
J. L. Beverly ◽  
D. W. Gietzen ◽  
Q. R. Rogers
Keyword(s):  
Amino Acids ◽  
Cerebrospinal Fluid ◽  
Amino Acid ◽  
Essential Amino Acid ◽  
Dark Period ◽  
Essential Amino Acids ◽  
Dark Phase ◽  
Artificial Cerebrospinal Fluid ◽  
Limiting Amino Acid ◽  
Prepyriform Cortex

Microinjection of the dietary limiting essential amino acid (DLAA) into the prepyriform cortex (PPC) increased intake of a diet having an imbalance among the essential amino acids (imbalanced diet) from 50-55% of baseline, when artificial cerebrospinal fluid (aCSF) was injected, to 70-75% of baseline. The increase in intake of the imbalanced diet by DLAA injection became apparent after 3-6 h and was maintained throughout the dark period. Meal size, meal duration, and the number of meals returned to normal after bilateral injections of the DLAA into the PPC of rats fed the imbalanced diet. Injection of the DLAA 30 min before the onset of the dark phase increased intake of imbalanced diet to 70% of baseline intake. When injections of threonine or isoleucine were made 6 and 3 h, respectively, prior to onset of the dark phase, intake of imbalanced diet increased to 85% of baseline intake. Results suggest that some form of processing of the injected DLAA within the PPC is necessary to increase the intake of imbalanced diets.

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