scholarly journals Morpho-Physiological Diversity and Its Association with Herbicide Resistance in Echinochloa Ecotypes

Weed Science ◽  
2021 ◽  
pp. 1-29
Author(s):  
Rui Liu ◽  
Vijay Singh ◽  
Seth Abugho ◽  
Hao-Sheng Lin ◽  
Xin-Gen Zhou ◽  
...  

Abstract The genus Echinochloa constitutes some of the most prominent weed species found in rice (Oryza sativa L.) production worldwide. The taxonomy of Echinochloa is complex due to its morphological variations. The morpho-physiological diversity and taxonomic characteristics of Echinochloa ecotypes infesting rice fields in Texas are unknown. A total of 54 Echinochloa ecotypes collected during late-season field surveys in 2015 and 2016 were characterized in a common garden in 2017. Plants were characterized for 14 morpho-physiological traits including stem angle; stem color; plant height; leaf color; leaf texture; flag leaf length, width, and angle; days to flowering; panicle length; plant biomass; seed shattering; seed yield; and seed dormancy. Principal component analysis indicated that four (plant height, flag leaf length, seed shattering, and seed germination) of the 14 phenological traits characterized here had significantly contributed to the overall morphological diversity of Echinochloa spp. Results showed wide inter-population diversity for the measured traits among the E. colona ecotypes, as well as diverse intra-population variability in all three Echinochola species studied, including barnyardgrass [Echinochloa crus-galli (L.) P. Beauv.], junglerice [Echinochloa colona (L.) Link], and rough barnyardgrass [Echinochloa muricata (P. Beauv.) Fernald]. Taxonomical classification revealed that the collection consisted of three Echinochloa species, with E. colona being the most dominant (96%), followed by E. crus-galli (2%), and E. muricata (2%). Correlation analysis of morpho-physiological traits and resistance status to commonly used preemergence (clomazone, quinclorac) and postemergence herbicides (propanil, quinclorac, imazethapyr, and fenoxaprop-ethyl) failed to show any significant association. Findings from this study provided novel insights into the morpho-physiological characteristics of Echinochloa ecotypes in rice production in Texas. The morphological diversity currently present in Echinochloa ecotypes could contribute to their adaptation to selection pressure imposed by different management tools, emphasizing the need for a diversified management approach to effectively control this weed species.

Author(s):  
D.R. Meghawal ◽  
G.M. Lal ◽  
Ranjana Tiwari

The present investigation consists of 34 genotypes with one local check (NDR-359) grown at field experimentation centre at the Department of Genetic and Plant Breeding, SHIATS, Allahabad evaluated for thirteen quantitative characters. All the genotypes differed significantly with respect to all the quantitative traits. High to moderate estimates of GCV and PCV were found for number of panicle per plant, number of tiller per plant, plant height and number of spikelet’s per panicle, indicating these traits could be use for selection in crop improvement. Plant height, number of spikelet’s per panicle, test weight and flag leaf length exhibited high values for broad sense heritability. Number of spikelet’s and plant height showed highest genetic advance coupled with high heritability, which is also exhibited by number of panicle per plant, number of tillers per plant and plant height suggesting pre dominance of additive gene action in the expression of these traits.


2018 ◽  
Vol 1 (02) ◽  
pp. 36-43
Author(s):  
S Sandamal ◽  
A Tennakoon ◽  
D Ratnasekera ◽  
DABN Amarasekera ◽  
B Marambe

Wild species of rice (Oryza) have superior agronomic characteristics to be incorporated in rice breeding programs worldwide.  Population studies of wild relatives of rice in Sri Lanka has not being well documented despite a few of attempts. In the present study, phenotypic diversity of Oryza rufipogon populations exist in Sri Lanka were characterized based on nine quantitative morphological traits. Populations (P1, P2, P3, P4 and P5) were established in a common-garden and were characterized. The results revealed moderate phenotypic diversity among O. rufipogon populations studied. However, flag leaf length and awn length were the most variable traits while plant height, flag leaf angle, flag leaf panicle neck length and spikelet angle were the least variable traits. O. rufipogon can be simply distinguished using flag leaf length and width, panicle branching type and distance from panicle base to lowest spikelet insertion. The dendrogram results indicated that four main clusters are at a similarity level of 98.73, showing the diversely related populations with a high identity based on higher similarity values. P1 and P2 populations grouped together by forming the first cluster. The second, third and fourth clusters consisted of P3, P5 and P4 populations, respectively. One population from first cluster and P3, P5 and P4 populations can be used for conservation. This study highlights the phenotypic diversity of O. rufipogon populations existing in Sri Lanka across the geographic locations and Knowledge on such morphological diversity provides opportunities to design conservation strategies and the potentials of using particular population based on breeding objectives.  


Author(s):  
Priyamvada Chauhan ◽  
Pradeep Kumar Pandey

Background: Selection of genotypically superior parents is most challenging task for the development of potential hybrids. In conventional plant breeding, magnitude of heterosis and inbreeding depression has been proved very effective in identifying vigorous parental combinations. Methods: The present investigation was carried out to study the heterosis, residual heterosis and inbreeding depression of forty sorghum crosses (F1s and F2s) obtained by crossing of eight restorer lines with five cytoplasmic male sterile lines in Line x Tester mating design in 2013 and 2014. Observations were recorded on morphological traits viz. days to 50% flowering, days to maturity, plant height, flag leaf length, flag leaf width, panicle length, panicle width, panicle weight,1000 seed weight and grain yield per plant. Result: Among all crosses, ICSA467 x UPC2 and MR750A2 x UPC2 were found to be best for most of the characters. Inbreeding depression was recorded as significantly positive for plant height for most of the crosses indicates the occurrence of transgressive segregants in F2 generation.


2020 ◽  
Vol 21 (17) ◽  
pp. 6170 ◽  
Author(s):  
Gul Nawaz ◽  
Babar Usman ◽  
Neng Zhao ◽  
Yue Han ◽  
Zhihua Li ◽  
...  

In rice, semi-dwarfism is among the most required characteristics, as it facilitates better yields and offers lodging resistance. Here, semi-dwarf rice lines lacking any residual transgene-DNA and off-target effects were generated through CRISPR/Cas9-guided mutagenesis of the OsGA20ox2 gene in a high yielding Basmati rice line, and the isobaric tags for relative and absolute quantification (iTRAQ) strategy was utilized to elucidate the proteomic changes in mutants. The results indicated the reduced gibberellins (GA1 and GA4) levels, plant height (28.72%), and flag leaf length, while all the other traits remained unchanged. The OsGA20ox2 expression was highly suppressed, and the mutants exhibited decreased cell length, width, and restored their plant height by exogenous GA3 treatment. Comparative proteomics of the wild-type and homozygous mutant line (GXU43_9) showed an altered level of 588 proteins, 273 upregulated and 315 downregulated, respectively. The identified differentially expressed proteins (DEPs) were mainly enriched in the carbon metabolism and fixation, glycolysis/gluconeogenesis, photosynthesis, and oxidative phosphorylation pathways. The proteins (Q6AWY7, Q6AWY2, Q9FRG8, Q6EPP9, Q6AWX8) associated with growth-regulating factors (GRF2, GRF7, GRF9, GRF10, and GRF11) and GA (Q8RZ73, Q9AS97, Q69VG1, Q8LNJ6, Q0JH50, and Q5MQ85) were downregulated, while the abscisic stress-ripening protein 5 (ASR5) and abscisic acid receptor (PYL5) were upregulated in mutant lines. We integrated CRISPR/Cas9 with proteomic screening as the most reliable strategy for rapid assessment of the CRISPR experiments outcomes.


2015 ◽  
Vol 7 (2) ◽  
pp. 836-838
Author(s):  
A. P. Goswami ◽  
B. Prasad ◽  
V. C. Joshi

The trial was conducted at the research block of Crop Improvement, GBPUAT, Hill Campus, Ranichauri using randomized block design (RBD) to characterize finger millet germplasm for morphological characters viz., plant height, flag leaf length, number of tiller plant-1, number of finger ear-1, ear length, no. of grain finger-1, no. of grain ear-1 and grain yield plant-1. Among all germplasms, number of finger ear-1, number of grain finger-1 and grain yield plant-1(g) had recorded highest in VL 149 which were 9.96, 150.66, 2.63 g respectively. The germplasm GEC 1406 attained lowest plant height (75.89 cm), GEC 961 had recorded higher flag leaf length (40.96 cm), GEC 268 had recorded maximum number of tiller plant-1 (3.30), GEC 199 had recorded higher ear length (9.20 cm), GEC 1044 had recorded maximum number of grain ear-1 (663) among all germplasm of finger millet. This study is helpful to identify superior germplasm so they can be used for further finger millet crop improvement programs.


Author(s):  
K. Rukmini Devi ◽  
B. Satish Chandra ◽  
N. Lingaiah ◽  
Y. Hari ◽  
V. Venkanna

Variability, correlation and path analysis for yield and quality traits were studied on 27 rice genotypes. The higher estimates of PCV and GCV were observed for yield per plant (42.04) and filled seeds per panicle (33.9) indicate possibility of genetic improvement through direct selection. High heritability in broad sense coupled with high genetic advance as percent of mean exhibited by effective tillers, plant height, flag leaf length, filled grains per panicle, test weight, yield per plant, head rice recovery and length/breadth ratio indicating preponderance of additive gene action which provide good scope for further improvement by selection. Grain yield per plant had highest significant positive association with filled seeds per panicle, plant height, flag leaf length, effective tillers, flag leaf width and panicle length indicating importance of these characters for yield improvement, while head rice recovery was found to be significantly and positively correlated with milling percent and hulling percent . Path analysis reveals that test weight (3.48), effective tillers (1.57), and filled grains per panicle (1.41) had positive direct effect on grain yield per plant. Among the quality traits kernel length followed by milling percent and kernel elongation ratio had direct effect on head rice recovery.


2022 ◽  
Vol 335 ◽  
pp. 00020
Author(s):  
Harmini Harmini ◽  
Achmad Fanindi ◽  
Endang Sutedi ◽  
Iwan Herdiawan ◽  
Sajimin Sajimin

Sorghum is a grain plant that is a source of food, biofuel and feed, which can be produced using the techniques of ratoon cultivation. The objective of this study is to assess the nutrition content, the performance of the four sorghum genotypes and the vegetative characteristics of the sorghum ratoon. The research was carried out from December 2020 to July 2021 at the Ciawi Research Garden, the Livestock Research Institute. The study employed four treatments (genotype) and three replications of random block design. The range of protein content, crude fat crude fiber and ash in the four genotypes of sorghum was 7.91-9.30, 1.91-2.69, 33.41-37.57 and 8.25-9.11%, respectively. Genotype significantly affected P<0.05 on plant height, stem height, leaf width, internode length, flag leaf width and fresh weight, but not significantly different P>0.05 on leaf length, stem diameter and flag leaf length on the main plant. Genotype had no significant effect (P>0.05) on plant height, number of tillers, leaf length, leaf width and fresh weight of ratoons. It was concluded that the ability of ratoon in sorghum was not affected by the genotype of sorghum.


2021 ◽  
pp. 1-10
Author(s):  
Yuki Makino ◽  
Yoshihiro Hirooka ◽  
Koki Homma ◽  
Rintaro Kondo ◽  
Tian-Sheng Liu ◽  
...  

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ali Muhammad ◽  
Jianguo Li ◽  
Weichen Hu ◽  
Jinsheng Yu ◽  
Shahid Ullah Khan ◽  
...  

AbstractWheat is a major food crop worldwide. The plant architecture is a complex trait mostly influenced by plant height, tiller number, and leaf morphology. Plant height plays a crucial role in lodging and thus affects yield and grain quality. In this study, a wheat population was genotyped by using Illumina iSelect 90K single nucleotide polymorphism (SNP) assay and finally 22,905 high-quality SNPs were used to perform a genome-wide association study (GWAS) for plant architectural traits employing four multi-locus GWAS (ML-GWAS) and three single-locus GWAS (SL-GWAS) models. As a result, 174 and 97 significant SNPs controlling plant architectural traits were detected by ML-GWAS and SL-GWAS methods, respectively. Among these SNP makers, 43 SNPs were consistently detected, including seven across multiple environments and 36 across multiple methods. Interestingly, five SNPs (Kukri_c34553_89, RAC875_c8121_1490, wsnp_Ex_rep_c66315_64480362, Ku_c5191_340, and tplb0049a09_1302) consistently detected across multiple environments and methods, played a role in modulating both plant height and flag leaf length. Furthermore, candidate SNPs (BS00068592_51, Kukri_c4750_452 and BS00022127_51) constantly repeated in different years and methods associated with flag leaf width and number of tillers. We also detected several SNPs (Jagger_c6772_80, RAC875_c8121_1490, BS00089954_51, Excalibur_01167_1207, and Ku_c5191_340) having common associations with more than one trait across multiple environments. By further appraising these GWAS methods, the pLARmEB and FarmCPU models outperformed in SNP detection compared to the other ML-GWAS and SL-GWAS methods, respectively. Totally, 152 candidate genes were found to be likely involved in plant growth and development. These finding will be helpful for better understanding of the genetic mechanism of architectural traits in wheat.


ISRN Genetics ◽  
2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
K. Medhabati ◽  
K. Rajiv Das ◽  
M. Rohinikumar ◽  
H. Sunitibala ◽  
Th. Dikash Singh

Genetic divergence of 32 indigenous rice germplasms and five wild rice of which three from Manipur and two wild rice procured from IRRI, Philippines was investigated using Mahalanobis, D2 statistic. Based on twelve agromorphological characters, the thirty-seven germplasms both wild and cultivated were grouped into five clusters based on the relative magnitudes of D2 values following Tocher's method of cluster formation. Based on the rank totals, the characters which contributed maximum towards genetic divergence in the present studies were grain yield/plant, spikelet/panicle, 100 grain weight, grain length, days to 50% flowering, ear bearing tillers/plant, and flag leaf length. In the present study, maximum intercluster distance was estimated between cluster III and (D2=14.09) which was closed followed by clusters II and V (D2=12.50). On the basis of their greater intercluster distance, high value of cluster mean according to the character to be improved and performance of the individual germplasms for the character, the germplasms could be used in hybridization programme for improvement of different plant characters in the rice germplasms of Manipur.


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