The Contribution of Sub-Aleurone Cells to Wheat Endosperm Protein Content and Gradient Is Dependent on Cultivar and N-Fertilization Level

Author(s):  
Wisse Hermans ◽  
Selime Mutlu ◽  
Adam Michalski ◽  
Niels A. Langenaeken ◽  
Christophe M. Courtin
1976 ◽  
Vol 86 (1) ◽  
pp. 155-161 ◽  
Author(s):  
A. Hadjichristodoulou

SUMMARYThe effect of stage of harvesting on dry-matter (D.M.) yield and chemical composition of barley, wheat and the legumes common vetch (F. sativa), woollypod vetch (F. dasycarpa) and fodder peas (P. sativum) were studied in Cyprus under low rainfall conditions in a series of trials sown in four successive years. Cereals were harvested at the beginning of heading, 50% heading and the milk stage of grain, and legumes at three stages from preflowering to full pod formation, D.M., protein and digestible D.M. yields and percentage D.M. content increased with age, whereas percentage protein content and D.M. digestibility declined. Under moisture stress conditions before and during the harvesting period D.M. yields did not increase significantly with age. Protein content of cereals under low rainfall conditions was higher than that of cereals grown in the U.K. under higher N fertilization levels. Rainfall conditions affected drastically the performance of both cereals and legumes. However, average yields were satisfactory; the barley variety 628 gave 8·98 t/ha, the highest D.M. yield among all cereal and legume varieties.


2012 ◽  
pp. 101-104
Author(s):  
Ágnes Krivián ◽  
Mihály Sárvári

The yielding capacity and quality parameters of 11 maize hybrids were studied in 2011 on calcareous chernozem soil in a 25-year long-term fertilization experiment in the control (without fertilization), in the base treatment of N 40 kg ha-1, P2O5 25 kg ha-1, K2O 30 kg ha-1 and in five treatments which were the multiplied doses of the base treatment. The N fertilizer was applied in the autumn and in the spring, while P and K fertilizers were applied in the autumn.The sowing time was 17–18 April, the time of harvest was 8 October. The 30-year average of precipitation (April–Sept) was 345.1 mm, the amount of precipitation did not differ greatly from that, however, its distribution was very unfavourable.It was found that the largest yield increment (as compared to the control) was in the treatment N 40 kg ha-1, P2O5 25 kg ha-1, K2O 30 kg ha-1 in the long-term experiment. The largest yields were obtained for the hybrids P9494, PR37N01 and PR35F38 (13.64–13.71 t ha-1). Due to the dry period at the end of the summer – beginning of autumn, the grain moisture content at harvest was favourably low, 12–18% depending on the treatment and the growing season. The N fertilization significantly increased the protein content of the kernel, but the starch content of the kernel decreased (significantly in several cases) with increasing fertilizer doses and yields as compared with the control.The highest protein content was measured in hybrids GK Boglár and Szegedi 386. The oil content was above 4% for GK Boglár, but the two hybrids were not among the best yielding hybrids in spite of their good inner content. The starch content was around 75 % without fertilization, it decreased with fertilization.For the tested hybrids, the fertilizer dose N 120 kg ha-1, P2O5 75 kg ha-1, K2O 90 kg ha-1 can be recommended with respect to efficacy and environmental considerations.


Author(s):  
Mitiku Woldesenbet ◽  
Asnakech Haileyesus

Maize response to high nitrogenous fertilization levels is a means among other means to know maximum productivity, from this perspective, a field nitrogen management trial using five N levels (0, 23, 46, 69 and 92 kg N/ha) with three replications. The study was conducted in 2015 in Decha District, ModyoGomberaKebele of Kaffa Zone, SNNPR State. The experiment was laid out in RCBD. The result of this study indicated that effects of different rates of N fertilizer had influenced the growth and yield components of maize. The tallest plant (360.66 cm) was recorded from the application of 92 kg N ha-1 and the shortest (347.33 cm) from no N application. The ANoVA for the number of kernels per ear showed that the lowest kernels per ear (497.86) were obtained from no N application and the highest kernels per ear (588) were obtained from the application of 92 kg N ha-1 although there was no significant difference between the application of 69 and 92 kg N ha-1. Regarding to ear length the data showed that the longest ear (23.63 cm) was obtained from the application of 92 kg N ha-1. The effect of N on  grain yield indicated that there is no significant difference between the application of 69 and 92 kg N ha-1 even if there is a slight difference on yield. Generally, maximum N fertilization level (92 Kg N/ha) in this study area showed increase in growth and yield components (number of kernels per ear and ear length). However the application of 69 kg N ha-1 seems adequate to get the optimum yield.


Agronomy ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 495 ◽  
Author(s):  
Marie Reimer ◽  
Björn Ringselle ◽  
Göran Bergkvist ◽  
Sally Westaway ◽  
Raphaël Wittwer ◽  
...  

Reducing soil tillage can lead to many benefits, but this practice often increases weed abundance and thus the need for herbicides, especially during the transition phase from inversion tillage to non-inversion tillage. We evaluated if subsidiary crops (SCs, e.g., cover crops) can mitigate the effects of non-inversion tillage on weed abundance. Two-year experiments studying SC use, tillage intensity, and nitrogen (N) fertilization level were carried out twice at six sites throughout northern and central Europe. SCs significantly reduced weed cover throughout the intercrop period (−55% to −1% depending on site), but only slightly during the main crops. Overall weed abundance and weed biomass were higher when using non-inversion tillage with SCs compared to inversion tillage without SCs. The effects differed due to site-specific weed pressure and management. With increasing weed pressure, the effect of SCs decreased, and the advantage of inversion over non-inversion tillage increased. N fertilization level did not affect weed abundance. The results suggest that SCs can contribute by controlling weeds but cannot fully compensate for reduced weed control of non-inversion tillage in the transition phase. Using non-inversion tillage together with SCs is primarily recommended in low weed pressure environments.


1982 ◽  
Vol 62 (1) ◽  
pp. 21-30 ◽  
Author(s):  
JOHN E. RICHARDS ◽  
R. J. SOPER

The effect of fertilizer N on yield, protein content and symbiotic N2 fixation in faba beans (Vicia faba L.) was studied with nine field trials during 3 yr. Forage and seed yields of nodulated faba beans were infrequently and unpredictably affected by rate of application at seeding (up to 300 kg N/ha), type of application (34 and 67 kg N/ha surface-broadcast or placed with the seed), and time of application (at full bloom or at mid pod-fill). Protein content of faba bean forage material was variably affected by applied N; in one-half of the harvests conducted, at least one of the N application treatments resulted in significantly higher forage protein contents. No logical pattern or reason for the forage protein response was found. Seed protein content was unaffected by applied N up to 150 kg N/ha, but was significantly increased by 300 kg N/ha in two out of three trials. Maximum rate of dry matter accumulation and N uptake commenced at blooming and continued up to the mid pod-fill growth stage. In seven trials monitored, the proportion of faba bean shoot N derived by symbiotic N2 fixation was 54%, with values of five out of the seven trials ranging from 63 to 71%. This amounted to 54–111 kg N/ha. Applied N decreased faba bean symbiotic fixation at all trials but one where available soil N was very high and symbiotic N2 was not occurring.


2021 ◽  
Vol 12 ◽  
Author(s):  
Songhe Chen ◽  
Xiaoling Xiang ◽  
Hongliang Ma ◽  
Petri Penttinen ◽  
Jiarong Zhao ◽  
...  

Diazotrophs that carry out the biological fixation of atmospheric dinitrogen (N2) replenish biologically available nitrogen (N) in soil and are influenced by the input of inorganic and organic substrates. To date, little is known about the effects of combined organic substrate addition and N fertilization on the diazotroph community composition and structure in purple soils. We investigated the effects of N fertilization and straw mulching on diazotroph communities by quantifying and sequencing the nifH gene in wheat rhizosphere. The abundance and richness of diazotrophs were greater the higher the fertilization level in the mulched treatments, whereas in the nonmulched treatments (NSMs), richness was lowest with the highest N fertilization level. The abundance and α-diversity of diazotrophs correlated with most of the soil properties but not with pH. At the genus level, the relative abundances of Azospirillum, Bacillus, and Geobacter were higher in the NSMs and those of Pseudacidovorax, Skermanella, Azospira, Paraburkholderia, Azotobacter, Desulfovibrio, Klebsiella, and Pelomonas in the mulched treatments. The differences in community composition between the mulched and the NSMs were associated with differences in soil temperature and soil organic carbon and available potassium contents and C:N ratio. Overall, straw mulching and N fertilization were associated with changes in diazotroph community composition and higher abundance of nifH gene in alkaline purple soils.


2007 ◽  
Vol 55 (1) ◽  
pp. 37-48 ◽  
Author(s):  
B. Varga ◽  
Z. Svečnjak ◽  
Z. Jurković ◽  
M. Pospišil

Winter wheat ( Triticum aestivum L.) cultivars may differ in grain quality responses to nitrogen (N) and fungicide applications, the two most important management inputs in the temperate climates of Europe. Limited information is available on N and fungicide effects on wheat quality in Croatia, where the wheat crop is widely grown under low N inputs without fungicide application. Field experiments were conducted during three years to evaluate the effects of fungicide (tebuconazol applied around heading) and two N fertilization rates on the grain quality of six widely grown winter wheat cultivars. Most cultivars failed to achieve the minimum breadmaking standards at a low N rate because of low protein content (103 g kg −1 ), Zeleny sedimentation (22.2 cm 3 ) and wet gluten (201 g kg −1 ). High N fertilization significantly increased these quality traits in all cultivars (an average of 21% for grain protein, 58% for Zeleny sedimentation and 40% for wet gluten). However, some cultivars with low genetic potential for accumulating grain protein failed to reach the breadmaking quality even at a high N rate when the N availability in the soil was limited by drought. Significant cultivar × N interactions existed for all grain quality traits, but were mainly associated with differences in the magnitude of the responses and less with the ranks. Five cultivars consistently showed increased falling number as the N rate rose, but these cultivar-specific responses to N fertilization were of much lesser magnitude than those across years. Fungicide application showed no effect on grain protein. Zeleny sedimentation, wet gluten or gluten index in all six cultivars tested, whereas one cultivar consistently showed decreased falling number after fungicide use. Only the hectolitre weights increased following fungicide application, especially for disease-susceptible cultivars at the high N rate. Thus, high N fertilization resulted in cultivar-dependent increases in protein content, Zeleny sedimentation, wet gluten and falling number, whereas fungicide application had no effect on grain quality except improved hectolitre weights.


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Matthew J. Milner ◽  
Sarah Bowden ◽  
Melanie Craze ◽  
Emma J. Wallington

Abstract Background Grain size is thought to be a major component of yield in many plant species. Here we set out to understand if knowledge from other cereals such as rice could translate to increased yield gains in wheat and lead to increased nitrogen use efficiency. Previous findings that the overexpression of OsBG1 in rice increased yields while increasing seed size suggest translating gains from rice to other cereals may help to increase yields. Results The orthologous genes of OsBG1 were identified in wheat. One homoeologous wheat gene was cloned and overexpressed in wheat to understand its role in controlling seed size. Potential alteration in the nutritional profile of the grains were also analyzed in wheat overexpressing TaBG1. It was found that increased TaBG1-A expression could indeed lead to larger seed size but was linked to a reduction in seed number per plant leading to no significant overall increase in yield. Other important components of yield such as biomass or tillering did not change significantly with increased TaBG1-A expression. The nutritional profile of the grain was altered, with a significant decrease in the Zn levels in the grain associated with increased seed size, but Fe and Mn concentrations were unchanged. Protein content of the wheat grain also fell under moderate N fertilization levels but not under deficient or adequate levels of N. Conclusions TaBG1 does control seed size in wheat but increasing the seed size per se does not increase yield and may come at the cost of lower concentrations of essential elements as well as potentially lower protein content. Nevertheless, TaBG1 could be a useful target for further breeding efforts in combination with other genes for increased biomass.


Agronomy ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2492
Author(s):  
Dietmar Meyer ◽  
Hartmut Kolbe

Organic matter (OM) and nutrient nitrogen (N) play vital roles in the fertility and production of soil in accordance with goals of efficient environmental protection. This study aimed to show the extent to which N delivery can contribute to improving nitrogen fertilizer requirements (NFR) through comparative analysis of OM and N. Systems determining the NFR in agricultural practices have thus far been challenged to estimate the annual rate of mineralization of the soil. OM and N turnover was investigated through an available evaluation consisting of 546 representatively distributed permanent test and observation plots (TP) of the German Federal State of Saxony farms. A solid database of at least 10-year field plot card records from 2001 to 2010 was selected for the analysis. A program (BEFU) widely used in agricultural practice, along with the simplified process model CCB, were applied. For the calculation of the amount of mineral N fertilizers used, the results of three different methods for determining the NFR were compared with each other. The determination of the farmers’ demand (=actual condition of the TP) with a mean value of 132 kg N ha−1 did not show a large difference between the calculated values with 137 kg N ha−1 by the BEFU program. Based on the available results for the most important crop species cultivated in Saxony, there were clear differences in the considerations of the N delivery from the soil. The BEFU program was able to calculate an average N delivery of 17 kg N ha−1 from tabulated data, whereas with the CCB process model, 66 kg N ha−1 of mineralization was determined with a distinct higher deviation by taking into account the 10-year field histories. Using the N delivery of the TP by the CCB model, a clear reduction of the mean N fertilization level, to about 80 kg N ha−1, was therefore achieved. These differences were particularly large for TP with organic fertilization (livestock), at a relatively low N fertilization level, and for certain crop species. With a high standard deviation, the average savings potential of mineral N fertilizers was 52–57 kg N ha−1. After including the corrected values for the N mineral fertilization, a decrease in the N balances by an average of 20–25 kg N ha−1 was ultimately achieved. In particular, the heavily oversupplied plots with D and E classification decreased by approximately 50%. The results of our study demonstrate clear improvements; therefore, increased efforts should be made in the future to optimize the determination of NFR using applicable methods that consider N mineralization in agricultural practice and consultation.


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