scholarly journals Competitive traits of coral symbionts may alter the structure and function of the microbiome

2020 ◽  
Vol 14 (10) ◽  
pp. 2424-2432
Author(s):  
Shelby E. McIlroy ◽  
Jane C. Y. Wong ◽  
David M. Baker
Keyword(s):  
Temperature Stress ◽  
Thermal Tolerance ◽  
Niche Partitioning ◽  
Cell Replication ◽  
Algal Symbionts ◽  
Nutrient Assimilation ◽  
The Face ◽  
Increased Sensitivity ◽  
Species Specific ◽  
And Function

Abstract In the face of global warming and unprecedented coral bleaching, a new avenue of research is focused on relatively rare algal symbionts and their ability to confer thermal tolerance to their host by association. Yet, thermal tolerance is just one of many physiological attributes inherent to the diversity of symbiodinians, a result of millions of years of competition and niche partitioning. Here, we revealed that competition among cocultured symbiodinians alters nutrient assimilation and compound production with species-specific responses. For Cladocopium goreaui, a species ubiquitous within stable coral associations, temperature stress increased sensitivity to competition eliciting a shift toward investment in cell replication, i.e., putative niche exploitation. Meanwhile, competition led Durusdinium trenchii, a thermally tolerant “background” symbiodinian, to divert resources from immediate growth to storage. As such, competition may be driving the dominance of C. goreaui outside of temperature stress, the destabilization of symbioses under thermal stress, the repopulation of coral tissues by D. trenchii following bleaching, and ultimately undermine the efficacy of symbiont turnover as an adaptive mechanism.

scholarly journals Molecular and phenotypic analysis of rodent models reveals conserved and species-specific modulators of human sarcopenia

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Anastasiya Börsch ◽  
Daniel J. Ham ◽  
Nitish Mittal ◽  
Lionel A. Tintignac ◽  
Eugenia Migliavacca ◽  
...  
Keyword(s):  
Muscle Mass ◽  
Inflammatory Responses ◽  
Molecular Data ◽  
Model Organisms ◽  
Sequencing Data ◽  
Phenotypic Analysis ◽  
Age Related ◽  
Analogous Data ◽  
Species Specific ◽  
And Function

AbstractSarcopenia, the age-related loss of skeletal muscle mass and function, affects 5–13% of individuals aged over 60 years. While rodents are widely-used model organisms, which aspects of sarcopenia are recapitulated in different animal models is unknown. Here we generated a time series of phenotypic measurements and RNA sequencing data in mouse gastrocnemius muscle and analyzed them alongside analogous data from rats and humans. We found that rodents recapitulate mitochondrial changes observed in human sarcopenia, while inflammatory responses are conserved at pathway but not gene level. Perturbations in the extracellular matrix are shared by rats, while mice recapitulate changes in RNA processing and autophagy. We inferred transcription regulators of early and late transcriptome changes, which could be targeted therapeutically. Our study demonstrates that phenotypic measurements, such as muscle mass, are better indicators of muscle health than chronological age and should be considered when analyzing aging-related molecular data.

scholarly journals Codon harmonization reduces amino acid misincorporation in bacterially expressed P. falciparum proteins and improves their immunogenicity

AMB Express ◽  
2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Neeraja Punde ◽  
Jennifer Kooken ◽  
Dagmar Leary ◽  
Patricia M. Legler ◽  
Evelina Angov
Keyword(s):  
Protein Structure ◽  
Amino Acid ◽  
Codon Usage ◽  
Dna Sequences ◽  
Structural Integrity ◽  
Host Cells ◽  
Loss Of Function ◽  
Species Specific ◽  
And Function ◽  
The Impact

Abstract Codon usage frequency influences protein structure and function. The frequency with which codons are used potentially impacts primary, secondary and tertiary protein structure. Poor expression, loss of function, insolubility, or truncation can result from species-specific differences in codon usage. “Codon harmonization” more closely aligns native codon usage frequencies with those of the expression host particularly within putative inter-domain segments where slower rates of translation may play a role in protein folding. Heterologous expression of Plasmodium falciparum genes in Escherichia coli has been a challenge due to their AT-rich codon bias and the highly repetitive DNA sequences. Here, codon harmonization was applied to the malarial antigen, CelTOS (Cell-traversal protein for ookinetes and sporozoites). CelTOS is a highly conserved P. falciparum protein involved in cellular traversal through mosquito and vertebrate host cells. It reversibly refolds after thermal denaturation making it a desirable malarial vaccine candidate. Protein expressed in E. coli from a codon harmonized sequence of P. falciparum CelTOS (CH-PfCelTOS) was compared with protein expressed from the native codon sequence (N-PfCelTOS) to assess the impact of codon usage on protein expression levels, solubility, yield, stability, structural integrity, recognition with CelTOS-specific mAbs and immunogenicity in mice. While the translated proteins were expected to be identical, the translated products produced from the codon-harmonized sequence differed in helical content and showed a smaller distribution of polypeptides in mass spectra indicating lower heterogeneity of the codon harmonized version and fewer amino acid misincorporations. Substitutions of hydrophobic-to-hydrophobic amino acid were observed more commonly than any other. CH-PfCelTOS induced significantly higher antibody levels compared with N-PfCelTOS; however, no significant differences in either IFN-γ or IL-4 cellular responses were detected between the two antigens.

Experimental analysis of the control of expression of the homeobox-gene Msx-1 in the developing limb and face

Development ◽  
1993 ◽  
Vol 119 (1) ◽  
pp. 41-48 ◽  
Author(s):  
J.M. Brown ◽  
S.E. Wedden ◽  
G.H. Millburn ◽  
L.G. Robson ◽  
R.E. Hill ◽  
...  
Keyword(s):  
Homeobox Gene ◽  
Host Tissue ◽  
Face To Face ◽  
Limb Mesenchyme ◽  
The Face ◽  
Mesenchyme Cells ◽  
Species Specific ◽  
Signalling Systems ◽  
Control Of Expression

Mouse mesenchyme was grafted into chick embryos to investigate the control of mesenchymal expression of Msx-1 in the developing limb and face. In situ hybridization, using species-specific probes, allows a comparison between Msx-1 expression in the graft and the host tissue. The results show that Msx-1 expression in both limb-to-limb and face-to-face grafts corresponds closely with the level of Msx-1 expression in the surrounding chick mesenchyme. Cells in grafts that end up within the host domain of Msx-1 express the gene irrespective of whether they were from normally expressing, or non-expressing, regions. Therefore Msx-1 expression in both the developing limb and the developing face appears to be position-dependent. Mesenchyme from each of the three major facial primordia behaved in the same way when grafted to the chick maxillary primordium. Reciprocal grafts between face and limb gave a different result: Msx-1 expression was activated when facial mesenchyme was grafted to the limb but not when limb mesenchyme was grafted to the face. This suggests either that there are quantitative or qualitative differences in two local signalling systems or that additional factors determine the responsiveness of the mesenchyme cells.

scholarly journals Neural contributors to trauma resilience: a review of longitudinal neuroimaging studies

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Alyssa R. Roeckner ◽  
Katelyn I. Oliver ◽  
Lauren A. M. Lebois ◽  
Sanne J. H. van Rooij ◽  
Jennifer S. Stevens
Keyword(s):  
Individual Differences ◽  
Neural Circuit ◽  
Major Life ◽  
Stress Resilience ◽  
Control Networks ◽  
The Face ◽  
Sensory Cortices ◽  
Life Stressor ◽  
Threat Cues ◽  
And Function

AbstractResilience in the face of major life stressors is changeable over time and with experience. Accordingly, differing sets of neurobiological factors may contribute to an adaptive stress response before, during, and after the stressor. Longitudinal studies are therefore particularly effective in answering questions about the determinants of resilience. Here we provide an overview of the rapidly-growing body of longitudinal neuroimaging research on stress resilience. Despite lingering gaps and limitations, these studies are beginning to reveal individual differences in neural circuit structure and function that appear protective against the emergence of future psychopathology following a major life stressor. Here we outline a neural circuit model of resilience to trauma. Specifically, pre-trauma biomarkers of resilience show that an ability to modulate activity within threat and salience networks predicts fewer stress-related symptoms. In contrast, early post-trauma biomarkers of subsequent resilience or recovery show a more complex pattern, spanning a number of major circuits including attention and cognitive control networks as well as primary sensory cortices. This novel synthesis suggests stress resilience may be scaffolded by stable individual differences in the processing of threat cues, and further buttressed by post-trauma adaptations to the stressor that encompass multiple mechanisms and circuits. More attention and resources supporting this work will inform the targets and timing of mechanistic resilience-boosting interventions.

scholarly journals NetFACS: Using network science to understand facial communication systems

2021 ◽  
Author(s):  
Alexander Mielke ◽  
Bridget M. Waller ◽  
Claire Pérez ◽  
Alan V. Rincon ◽  
Julie Duboscq ◽  
...  
Keyword(s):  
Network Analysis ◽  
Communication Systems ◽  
Network Science ◽  
Coding System ◽  
Communication Tool ◽  
Resampling Methods ◽  
Facial Movements ◽  
The Face ◽  
Analyse Data ◽  
And Function

AbstractUnderstanding facial signals in humans and other species is crucial for understanding the evolution, complexity, and function of the face as a communication tool. The Facial Action Coding System (FACS) enables researchers to measure facial movements accurately, but we currently lack tools to reliably analyse data and efficiently communicate results. Network analysis can provide a way to use the information encoded in FACS datasets: by treating individual AUs (the smallest units of facial movements) as nodes in a network and their co-occurrence as connections, we can analyse and visualise differences in the use of combinations of AUs in different conditions. Here, we present ‘NetFACS’, a statistical package that uses occurrence probabilities and resampling methods to answer questions about the use of AUs, AU combinations, and the facial communication system as a whole in humans and non-human animals. Using highly stereotyped facial signals as an example, we illustrate some of the current functionalities of NetFACS. We show that very few AUs are specific to certain stereotypical contexts; that AUs are not used independently from each other; that graph-level properties of stereotypical signals differ; and that clusters of AUs allow us to reconstruct facial signals, even when blind to the underlying conditions. The flexibility and widespread use of network analysis allows us to move away from studying facial signals as stereotyped expressions, and towards a dynamic and differentiated approach to facial communication.

The Changing Face and Function of Museums

1998 ◽  
Vol 89 (1) ◽  
pp. 47-61 ◽  
Author(s):  
Robin Trotter
Keyword(s):  
Material Culture ◽  
Identity Formation ◽  
Economic Forces ◽  
The Face ◽  
National Museums ◽  
And Function ◽  
A Site ◽  
Internal Pressures ◽  
New Museology

Museums are currently undergoing a number of changes as a repercussion of their histories and professional developments, and of new social and cultural contexts, not to mention as a result of pressure from competition and economic forces. This article explores the current ‘reinvention’ of museums — in particular, national museums — by examining some of the factors of change — some of the major internal pressures that have been the result of museological initiatives and also various exogenous influences. New museology and postcolonialism represent not only separate forces, but also a synthesis of pressures that are not only changing the face, but also the role, of museums, whilst also transforming relationships between museums and their users. A concern of this study is to look at those museums which have a ‘nationalising’ function, and to determine whether changing policies and practices are inhibiting or advance a renegotiation of the relationships between museums and their constituencies. In the last two decades, we have seen some trends confirmed. There has been a move from material culture studies to concern with the ideas contained in objects, whilst the older notion of the museum as a treasure house has given way to a stronger educative role and, more recently, an information centre and also a site of leisure, entertainment and identity-formation. These ‘reinventive’ processes, it is suggested, are closely allied to a postcolonial imperative.

scholarly journals Assessing the Translucence and Color-Change Methods for Estimating Sapwood Depth in Three Boreal Species

Forests ◽  
2018 ◽  
Vol 9 (11) ◽  
pp. 686 ◽  
Author(s):  
M. Quiñonez-Piñón ◽  
Caterina Valeo
Keyword(s):  
Populus Tremuloides ◽  
Picea Glauca ◽  
Pinus Banksiana ◽  
Wood Anatomy ◽  
Color Change ◽  
Microscopic Analysis ◽  
Forest Monitoring ◽  
Statistical Comparison ◽  
The Face ◽  
Species Specific

The translucence and color change in wood methods, which are commonly used to differentiate sapwood from heartwood in tree cores, are compared against the microscopic analysis of wood anatomy method for determining sapwood depth. The translucence method was tested on collected wood cores of White Spruce (Picea glauca (Moench) Voss) and Jack Pine (Pinus banksiana Lamb.). The color change in wood method was tested on Trembling Aspen (Populus tremuloides Michx.). For every statistical comparison, sapwood depth values obtained with the translucence or color-change methods were significantly different from those obtained using the microscopic analysis. Using the sapwood depth values obtained with microscopy as a reference, the bias associated with the translucence or color-change methods used on Picea glauca, Pinus banksiana and Populus tremuloides constantly under- or overestimated sapwood depths within −0.3 cm to 1.6 cm; −4.9 cm to 0.5 cm; and 0 to 1.8 cm, respectively. The different ranges of over- and underestimation arise from species-specific anatomical characteristics. Estimates for the errors in sapwood depth, when the depth is measured using either the translucence or color-change methods, are presented. These relationships and research outcomes will lead to more efficient forest monitoring and improved estimates of forest water balance, which in turn will lead to improved forest management in the face of climate change.

scholarly journals Improved Detection of Inundation below the Forest Canopy using Normalized LiDAR Intensity Data

2020 ◽  
Vol 12 (4) ◽  
pp. 707 ◽  
Author(s):  
Megan W. Lang ◽  
Vincent Kim ◽  
Gregory W. McCarty ◽  
Xia Li ◽  
In-Young Yeo ◽  
...  
Keyword(s):  
Forest Canopy ◽  
Deciduous Forest ◽  
Forested Wetlands ◽  
Evergreen Forest ◽  
Spatial And Temporal Patterns ◽  
Inundation Maps ◽  
The Face ◽  
Moderate Resolution ◽  
And Function ◽  
Normalization Process

To best conserve wetlands and manage associated ecosystem services in the face of climate and land-use change, wetlands must be routinely monitored to assess their extent and function. Wetland extent and function are largely driven by spatial and temporal patterns in inundation and soil moisture, which to date have been challenging to map, especially within forested wetlands. The objective of this paper is to investigate the different, but often interacting effects, of evergreen vegetation and inundation on leaf-off bare earth return lidar intensity within mixed deciduous-evergreen forests in the Coastal Plain of Maryland, and to develop an inundation mapping approach that is robust in areas of varying levels of evergreen influence. This was achieved through statistical comparison of field derived metrics, and development of a simple yet robust normalization process, based on first of many, and bare earth lidar intensity returns. Results demonstrate the confounding influence of forest canopy gap fraction and inundation, and the effectiveness of the normalization process. After normalization, inundated deciduous forest could be distinguished from non-inundated evergreen forest. Inundation was mapped with an overall accuracy between 99.4% and 100%. Inundation maps created using this approach provide insights into physical processes in support of environmental decision-making, and a vital link between fine-scale physical conditions and moderate resolution satellite imagery through enhanced calibration and validation.

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