scholarly journals Enhanced lipid production by addition of malic acid in fermentation of recombinant Mucor circinelloides Mc-MT-2

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yao Zhang ◽  
Qing Liu ◽  
Pengcheng Li ◽  
Yanxia Wang ◽  
Shaoqi Li ◽  
...  
Keyword(s):  
Malic Acid ◽  
Large Scale ◽  
Fatty Acid Biosynthesis ◽  
Lipid Production ◽  
Mucor Circinelloides ◽  
Industrial Applications ◽  
Vital Role ◽  
Transport Systems ◽  
Transporter Protein ◽  
Malate Transporter

AbstractIn our previous work, we reported a novel approach for increasing lipid production in an oleaginous fungus Mucor circinelloides by overexpression of mitochondrial malate transporter protein. This transporter plays a vital role in fatty acid biosynthesis during malate and citrate transport systems in oleaginous fungi. In this study, the controlling metabolic supplementation strategy was used to improve the lipid production by overexpression of malate transporter protein in M. circinelloides strain coded as Mc-MT-2. The effects of different metabolic intermediates on lipid production in batch fermentation by Mc-MT-2 were investigated. The optimal lipid production was obtained at 0.8% malic acid after 24 h of fermentation. Furthermore, in fed-batch bioreactors containing glucose as a carbon source supplemented with malic acid, the highest cell growth, and lipid production were achieved. The resulting strain showed the fungal dry biomass of 16 g/L, a lipid content of 32%, lipid yield of 5.12 g/L in a controlled bench-top bioreactor, with 1.60-, 1.60- and 2.56-fold improvement, respectively, compared with the batch control without supplementation of malic acid. Our findings revealed that the addition of malic acid during fermentation might play an important role in lipid accumulation in the recombinant M. circinelloides Mc-MT-2. This study provides valuable insights for enhanced microbial lipid production through metabolic supplementation strategy in large scale and industrial applications.

scholarly journals Investigation of Lipolytic-Secreting Bacteria from an Artificially Polluted Soil Using a Modified Culture Method and Optimization of Their Lipase Production

2021 ◽  
Vol 9 (12) ◽  
pp. 2590
Author(s):  
Van Hong Thi Pham ◽  
Jaisoo Kim ◽  
Soonwoong Chang ◽  
Woojin Chung
Keyword(s):  
Metal Ions ◽  
Large Scale ◽  
Nitrogen Sources ◽  
Culture Method ◽  
Lipase Production ◽  
Industrial Applications ◽  
Vital Role ◽  
Bacterial Strains ◽  
Functional Bacteria ◽  
Microbial Lipases

Compared to lipases from plants or animals, microbial lipases play a vital role in different industrial applications and biotechnological perspectives due to their high stability and cost-effectiveness. Therefore, numerous lipase producers have been investigated in a variety of environments in the presence of lipidic carbon and organic nitrogen sources. As a step in the development of cultivating the unculturable functional bacteria in this study, the forest soil collected from the surrounding plant roots was used to create an artificially contaminated environment for lipase-producing bacterial isolation. The ten strongest active bacterial strains were tested in an enzyme assay supplemented with metal ions such as Ca2+, Zn2+, Cu2+, Fe2+, Mg2+, K+, Co2+, Mn2+, and Sn2+ to determine bacterial tolerance and the effect of these metal ions on enzyme activity. Lipolytic bacteria in this study tended to grow and achieved a high lipase activity at temperatures of 35–40 °C and at pH 6–7, reaching a peak of 480 U/mL and 420 U/mL produced by Lysinibacillus PL33 and Lysinibacillus PL35, respectively. These potential lipase-producing bacteria are excellent candidates for large-scale applications in the future.

Development of Nanomaterials as Photo Catalysts for Environmental Applications

2020 ◽  
Vol 09 ◽  
Author(s):  
Ahmed M. Abu-Dief ◽  
W. S. Mohamed
Keyword(s):  
Water Purification ◽  
Smart Materials ◽  
Block Size ◽  
Industrial Applications ◽  
Vital Role ◽  
High Catalytic Activity ◽  
Chemical Contaminants ◽  
Environmental Threats ◽  
Photo Catalysis ◽  
Materials Used

Abstract:: Sustainability environmental lack is a growing and pivotal mater due to the issues: such as disturbances associated with biodiversity pollution, and climate change. Pollutants are the major cause of these environmental threats in the atmosphere. In recently, the nano-based photocatalyst is at the forefront of the author's interest because of its promising potential as a green chemical-based compound, high catalytic activity, the suitable and controllable surface area for wastewater treatment. Semiconductor materials in nanosized scale have electronic and optical properties depend on its building block size, which plays a vital role in developing smart materials that are well efficient for simultaneously destroying harmful chemical contaminants from our environment. This makes these materials used in many possible industrial applications such as water purification. In this Review, we report the most significant results contributing to progress in the area of environmental hazardous pollutant detection and removal focused on water purification especially through photo-catalysis to give readers an overview of the present research trends. Moreover, we analyze previous studies to indicate key principles of photo-catalysis and provide guidelines that can be used to fabricate more efficient photocatalysts.

scholarly journals Dissecting cellobiose metabolic pathway and its application in biorefinery through consolidated bioprocessing in Myceliophthora thermophila

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Jingen Li ◽  
Shuying Gu ◽  
Zhen Zhao ◽  
Bingchen Chen ◽  
Qian Liu ◽  
...  
Keyword(s):  
Malic Acid ◽  
Plant Biomass ◽  
Consolidated Bioprocessing ◽  
Cellulase Activity ◽  
Industrial Applications ◽  
Efficient Production ◽  
Myceliophthora Thermophila ◽  
Final Strain ◽  
Cellobiose Metabolism ◽  
Cost Efficient

Abstract Background Lignocellulosic biomass has long been recognized as a potential sustainable source for industrial applications. The costs associated with conversion of plant biomass to fermentable sugar represent a significant barrier to the production of cost-competitive biochemicals. Consolidated bioprocessing (CBP) is considered a potential breakthrough for achieving cost-efficient production of biomass-based fuels and commodity chemicals. During the degradation of cellulose, cellobiose (major end-product of cellulase activity) is catabolized by hydrolytic and phosphorolytic pathways in cellulolytic organisms. However, the details of the two intracellular cellobiose metabolism pathways in cellulolytic fungi remain to be uncovered. Results Using the engineered malic acid production fungal strain JG207, we demonstrated that the hydrolytic pathway by β-glucosidase and the phosphorolytic pathway by phosphorylase are both used for intracellular cellobiose metabolism in Myceliophthora thermophila, and the yield of malic acid can benefit from the energy advantages of phosphorolytic cleavage. There were obvious differences in regulation of the two cellobiose catabolic pathways depending on whether M. thermophila JG207 was grown on cellobiose or Avicel. Disruption of Mtcpp in strain JG207 led to decreased production of malic acid under cellobiose conditions, while expression levels of all three intracellular β-glucosidase genes were significantly up-regulated to rescue the impairment of the phosphorolytic pathway under Avicel conditions. When the flux of the hydrolytic pathway was reduced, we found that β-glucosidase encoded by bgl1 was the dominant enzyme in the hydrolytic pathway and deletion of bgl1 resulted in significant enhancement of protein secretion but reduction of malate production. Combining comprehensive manipulation of both cellobiose utilization pathways and enhancement of cellobiose uptake by overexpression of a cellobiose transporter, the final strain JG412Δbgl2Δbgl3 produced up to 101.2 g/L and 77.4 g/L malic acid from cellobiose and Avicel, respectively, which corresponded to respective yields of 1.35 g/g and 1.03 g/g, representing significant improvement over the starting strain JG207. Conclusions This is the first report of detailed investigation of intracellular cellobiose catabolism in cellulolytic fungus M. thermophila. These results provide insights that can be applied to industrial fungi for production of biofuels and biochemicals from cellobiose and cellulose.

scholarly journals A modular microfluidic system based on a multilayered configuration to generate large-scale perfusable microvascular networks

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Tao Yue ◽  
Da Zhao ◽  
Duc T. T. Phan ◽  
Xiaolin Wang ◽  
Joshua Jonghyun Park ◽  
...  
Keyword(s):  
Large Scale ◽  
Circulatory System ◽  
Vertical Direction ◽  
Microfluidic System ◽  
Vital Role ◽  
Microvascular Networks ◽  
Biological Studies ◽  
Wide Range ◽  
High Flexibility

AbstractThe vascular network of the circulatory system plays a vital role in maintaining homeostasis in the human body. In this paper, a novel modular microfluidic system with a vertical two-layered configuration is developed to generate large-scale perfused microvascular networks in vitro. The two-layer polydimethylsiloxane (PDMS) configuration allows the tissue chambers and medium channels not only to be designed and fabricated independently but also to be aligned and bonded accordingly. This method can produce a modular microfluidic system that has high flexibility and scalability to design an integrated platform with multiple perfused vascularized tissues with high densities. The medium channel was designed with a rhombic shape and fabricated to be semiclosed to form a capillary burst valve in the vertical direction, serving as the interface between the medium channels and tissue chambers. Angiogenesis and anastomosis at the vertical interface were successfully achieved by using different combinations of tissue chambers and medium channels. Various large-scale microvascular networks were generated and quantified in terms of vessel length and density. Minimal leakage of the perfused 70-kDa FITC-dextran confirmed the lumenization of the microvascular networks and the formation of tight vertical interconnections between the microvascular networks and medium channels in different structural layers. This platform enables the culturing of interconnected, large-scale perfused vascularized tissue networks with high density and scalability for a wide range of multiorgan-on-a-chip applications, including basic biological studies and drug screening.

scholarly journals On the relation between mini-halos and AGN feedback in clusters of galaxies

2020 ◽  
Vol 499 (2) ◽  
pp. 2934-2958
Author(s):  
A Richard-Laferrière ◽  
J Hlavacek-Larrondo ◽  
R S Nemmen ◽  
C L Rhea ◽  
G B Taylor ◽  
...  
Keyword(s):  
Large Scale ◽  
State Of The Art ◽  
Vital Role ◽  
Clusters Of Galaxies ◽  
Relativistic Jets ◽  
X Ray ◽  
Core Cluster ◽  
Central Galaxy ◽  
Central Regions ◽  
Radio Power

ABSTRACT A variety of large-scale diffuse radio structures have been identified in many clusters with the advent of new state-of-the-art facilities in radio astronomy. Among these diffuse radio structures, radio mini-halos are found in the central regions of cool core clusters. Their origin is still unknown and they are challenging to discover; less than 30 have been published to date. Based on new VLA observations, we confirmed the mini-halo in the massive strong cool core cluster PKS 0745−191 (z = 0.1028) and discovered one in the massive cool core cluster MACS J1447.4+0827 (z = 0.3755). Furthermore, using a detailed analysis of all known mini-halos, we explore the relation between mini-halos and active galactic nucleus (AGN) feedback processes from the central galaxy. We find evidence of strong, previously unknown correlations between mini-halo radio power and X-ray cavity power, and between mini-halo and the central galaxy radio power related to the relativistic jets when spectrally decomposing the AGN radio emission into a component for past outbursts and one for ongoing accretion. Overall, our study indicates that mini-halos are directly connected to the central AGN in clusters, following previous suppositions. We hypothesize that AGN feedback may be one of the dominant mechanisms giving rise to mini-halos by injecting energy into the intra-cluster medium and reaccelerating an old population of particles, while sloshing motion may drive the overall shape of mini-halos inside cold fronts. AGN feedback may therefore not only play a vital role in offsetting cooling in cool core clusters, but may also play a fundamental role in re-energizing non-thermal particles in clusters.

scholarly journals Time-resolved PIV measurements of a deflected submerged jet interacting with liquid-gas and liquid-liquid interfaces

2021 ◽  
Author(s):  
Stefan Puttinger ◽  
Mahdi Saeedipour
Keyword(s):  
Liquid Layer ◽  
Large Scale ◽  
Free Surface Flow ◽  
Industrial Applications ◽  
Liquid Pool ◽  
Surface Flow ◽  
Two Phase ◽  
Time Resolved ◽  
Submerged Jet ◽  
Major Interest

AbstractThis paper presents an experimental investigation on the interactions of a deflected submerged jet into a liquid pool with its above interface in the absence and presence of an additional lighter liquid. Whereas the former is a free surface flow, the latter mimics a situation of two stratified liquids where the liquid-liquid interface is disturbed by large-scale motions in the liquid pool. Such configurations are encountered in various industrial applications and, in most cases, it is of major interest to avoid the entrainment of droplets from the lighter liquid into the main flow. Therefore, it is important to understand the fluid dynamics in such configurations and to analyze the differences between the cases with and without the additional liquid layer. To study this problem, we applied time-resolved particle image velocimetry experiments with high spatial resolution. A detailed data analysis of a small layer beneath the interface shows that although the presence of an additional liquid layer stabilizes the oscillations of the submerged jet significantly, the amount of kinetic energy, enstrophy, and velocity fluctuations concentrated in the proximity of the interface is higher when the oil layer is present. In addition, we analyze the energy distribution across the eigenmodes of a proper orthogonal distribution and the distribution of strain and vortex dominated regions. As the main objective of this study, these high-resolution time-resolved experimental data provide a validation platform for the development of new models in the context of the volume of fluid-based large eddy simulation of turbulent two-phase flows.

scholarly journals The Sense of Smell and Aging: What We Have Learned From Population Studies

2020 ◽  
Vol 4 (Supplement_1) ◽  
pp. 810-811
Author(s):  
Jayant Pinto
Keyword(s):  
Mental Health ◽  
Older Adults ◽  
Social Life ◽  
Large Scale ◽  
Population Studies ◽  
Vital Role ◽  
Sensory Function ◽  
Physical And Mental Health ◽  
Sense Of Smell ◽  
Health Aging

Abstract Decline of the sense of smell with age causes a marked impact on older adults, markedly reducing quality of life. Olfactory dysfunction impairs nutrition, decreases the ability to experience pleasure, and results in depression, among other burdens. Large-scale population studies have identified impaired olfaction as a key heath indicator that predicts the development of decreased physical and mental health, reduced physical activity, weight loss, mild cognitive impairment and dementia, and mortality itself. These data have been generated via analyses of data from several aging cohorts, including the National Social Life, Health, and Aging Project (NSHAP); the Beaver Dam cohort; the Atherosclerosis Risk in Communities project; the Rush Memory and Aging Project; the Health, Aging, and Body Composition project; the Washington Heights/Inwood Columbia Aging Project; among others. In this presentation, we will review the close connection between olfaction, health, aging, including discussion of insights from these studies. We will also discuss emerging data from NSHAP on the effects of sensory function on cognition, mental health, and social interaction, which demonstrate that sensory function plays a vital role in the lives of older adults. Part of a symposium sponsored by Sensory Health Interest Group.

Research on a New Type of Lithium Battery String Equalization and Management System

2011 ◽  
Vol 383-390 ◽  
pp. 1470-1476
Author(s):  
Hao Wang ◽  
Ding Guo Shao ◽  
Lu Xu
Keyword(s):  
Lithium Batteries ◽  
Management System ◽  
Lithium Battery ◽  
Large Scale ◽  
Control Method ◽  
Balance Control ◽  
Industrial Applications ◽  
Control Technique ◽  
New Type ◽  
Management Functions

Lithium battery has been employed widely in many industrial applications. Parameter mismatches between lithium batteries along a series string is the critical limits of the large-scale applications in high power situation. Maintaining equalization between batteries is the key technique in lithium batteries application. This paper summarizes normal equalization techniques and proposed a new type of lithium Battery Equalization and Management System (BEMS) employing the isolated DC-DC converter structure. The system is integrated both equalization functions and management functions by using distributed 3-level controlled structure and digital control technique. With this control method the flexibility of the balance control strategy and the compatibility for different battery strings are both improved dramatically. The experimental results show optimizing equalization, efficiency and the battery string life span has been extended.

scholarly journals Functionalized Carbon Nanotubes (CNTs) for Water and Wastewater Treatment: Preparation to Application

2021 ◽  
Vol 13 (10) ◽  
pp. 5717
Author(s):  
Mian Muhammad-Ahson Aslam ◽  
Hsion-Wen Kuo ◽  
Walter Den ◽  
Muhammad Usman ◽  
Muhammad Sultan ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Wastewater Treatment ◽  
Water Resources ◽  
Large Scale ◽  
Industrial Applications ◽  
Biological Species ◽  
Special Focus ◽  
Water And Wastewater Treatment ◽  
Adsorption Sites ◽  
Water And Wastewater

As the world human population and industrialization keep growing, the water availability issue has forced scientists, engineers, and legislators of water supply industries to better manage water resources. Pollutant removals from wastewaters are crucial to ensure qualities of available water resources (including natural water bodies or reclaimed waters). Diverse techniques have been developed to deal with water quality concerns. Carbon based nanomaterials, especially carbon nanotubes (CNTs) with their high specific surface area and associated adsorption sites, have drawn a special focus in environmental applications, especially water and wastewater treatment. This critical review summarizes recent developments and adsorption behaviors of CNTs used to remove organics or heavy metal ions from contaminated waters via adsorption and inactivation of biological species associated with CNTs. Foci include CNTs synthesis, purification, and surface modifications or functionalization, followed by their characterization methods and the effect of water chemistry on adsorption capacities and removal mechanisms. Functionalized CNTs have been proven to be promising nanomaterials for the decontamination of waters due to their high adsorption capacity. However, most of the functional CNT applications are limited to lab-scale experiments only. Feasibility of their large-scale/industrial applications with cost-effective ways of synthesis and assessments of their toxicity with better simulating adsorption mechanisms still need to be studied.

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