Imaging of β-amyloid plaques by near infrared fluorescent tracers: a new frontier for chemical neuroscience

Anin vitroandin vivodrug-reporting system is developed for real-time monitoring of drug release via the analysis of the concurrently released near-infrared fluorescence dye.

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Novel quinoxaline derivatives for in vivo imaging of β-amyloid plaques in the brain

Bioorganic & Medicinal Chemistry Letters ◽

10.1016/j.bmcl.2011.05.079 ◽

2011 ◽

Vol 21 (14) ◽

pp. 4193-4196 ◽

Cited By ~ 18

Author(s):

Mengchao Cui ◽

Masahiro Ono ◽

Hiroyuki Kimura ◽

Boli Liu ◽

Hideo Saji

Keyword(s):

In Vivo Imaging ◽

Amyloid Plaques ◽

Quinoxaline Derivatives ◽

Β Amyloid ◽

The Brain

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Measurement of Peripheral Venous Oxygen Saturation in the Leg Using Near-Infrared Spectroscopy

Phlebology The Journal of Venous Disease ◽

10.1177/026835559701200305 ◽

1997 ◽

Vol 12 (3) ◽

pp. 96-99 ◽

Cited By ~ 2

Author(s):

D. Wertheim ◽

R. Salaman ◽

J. Melhuish ◽

R. Williams ◽

I. Lane ◽

...

Keyword(s):

Near Infrared ◽

Tissue Oxygenation ◽

Venous Occlusion ◽

Lower Leg ◽

Non Invasive ◽

Short Period ◽

Venous Oxygen Saturation ◽

Invasive Method ◽

Healing Of Wounds ◽

Total Haemoglobin

Background: It has been suggested that poor healing of wounds may be associated with reduced tissue oxygenation. A non-invasive method of assessing peripheral venous oxygenation has been investigated. Method: Changes in oxyhaemoglobin (O2Hb), deoxyhaemoglobin (HHb), oxidized cytochrome aa3 (cyt aa3) and total haemoglobin (tHb) were monitored in the left lower leg of seven healthy volunteers. A short period of venous occlusion was achieved by rapidly inflating a sphygmomanometer cuff placed around the leg to 60 mmHg. The changes in O2Hb and tHb, with respect to the baseline readings, were evaluated. PSvO2 was calculated from (ΔO2Hb/ΔtHb) × 100%. Results: From 17 sets of readings on the seven volunteers the median PSvO2 calculated was 64% (range 50–86%). Conclusion: This method appears to be a simple means of evaluating PSvO2. A change in cyt aa3 was often seen associated with the venous occlusion.

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Multiplexed Non-invasive in vivo Imaging to Assess Metabolism and Receptor Engagement in Tumor Xenografts

10.1101/758383 ◽

2019 ◽

Author(s):

Alena Rudkouskaya ◽

Nattawut Sinsuebphon ◽

Marien Ochoa ◽

Joe E. Mazurkiewicz ◽

Xavier Intes ◽

...

Keyword(s):

Drug Delivery ◽

Targeted Delivery ◽

Near Infrared ◽

Metabolic Response ◽

Imaging Modalities ◽

Wide Field ◽

Receptor Ligand ◽

Non Invasive ◽

Intracellular Drug Delivery

AbstractFollowing an ever-increased focus on personalized medicine, there is a continuing need to develop preclinical molecular imaging modalities to guide the development and optimization of targeted therapies. To date, non-invasive quantitative imaging modalities that can comprehensively assess simultaneous cellular drug delivery efficacy and therapeutic response are lacking. In this regard, Near-Infrared (NIR) Macroscopic Fluorescence Lifetime Förster Resonance Energy Transfer (MFLI-FRET) imaging offers a unique method to robustly quantify receptor-ligand engagement in vivo and subsequent intracellular internalization, which is critical to assess the delivery efficacy of targeted therapeutics. However, implementation of multiplexing optical imaging with FRET in vivo is challenging to achieve due to spectral crowding and cross-contamination. Herein, we report on a strategy that relies on a dark quencher that enables simultaneous assessment of receptor-ligand engagement and tumor metabolism in intact live mice. First, we establish that IRDye QC-1 (QC-1) is an effective NIR dark acceptor for the FRET-induced quenching of donor Alexa Fluor 700 (AF700) using in vitro NIR FLI microscopy and in vivo wide-field MFLI imaging. Second, we report on simultaneous in vivo imaging of the metabolic probe IRDye 800CW 2-deoxyglucose (2-DG) and MFLI-FRET imaging of NIR-labeled transferrin FRET pair (Tf-AF700/Tf-QC-1) uptake in tumors. Such multiplexed imaging revealed an inverse relationship between 2-DG uptake and Tf intracellular delivery, suggesting that 2-DG signal may predict the efficacy of intracellular targeted delivery. Overall, our methodology enables for the first time simultaneous non-invasive monitoring of intracellular drug delivery and metabolic response in preclinical studies.

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In vivo non-invasive near-infrared spectroscopy distinguishes normal, post-stroke, and botulinum toxin treated human muscles

Scientific Reports ◽

10.1038/s41598-021-96547-6 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Antonio Currà ◽

Riccardo Gasbarrone ◽

Alessandra Cardillo ◽

Francesco Fattapposta ◽

Paolo Missori ◽

...

Keyword(s):

Botulinum Toxin ◽

Near Infrared ◽

Comprehensive Evaluation ◽

Botulinum Toxin Injection ◽

Principal Component ◽

Short Wave ◽

Post Stroke ◽

Non Invasive ◽

Optimal Monitoring

AbstractIn post-stroke hemiparesis, neural impairment alters muscle control, causing abnormal movement and posture in the affected limbs. A decrease in voluntary use of the paretic arm and flexed posture during rest also induce secondary tissue transformation in the upper limb muscles. To obtain a specific, accurate, and reproducible marker of the current biological status of muscles, we collected visible (VIS) and short-wave Infrared (SWIR) reflectance spectra in vivo using a portable spectroradiometer (350–2500 nm), which provided the spectral fingerprints of the elbow flexors and extensors. We compared the spectra for the affected and unaffected sides in 23 patients with post-stroke hemiparesis (25–87 years, 8 women) and eight healthy controls (33–87 years, 5 women). In eight patients, spectra were collected before and after botulinum toxin injection. Spectra underwent off-line preprocessing, principal component analysis, and partial least-squares discriminant analysis. Spectral fingerprints discriminated the muscle (biceps vs. triceps), neurological condition (normal vs. affected vs. unaffected), and effect of botulinum toxin treatment (before vs. 30 to 40 days vs. 110 to 120 days after injection). VIS-SWIR spectroscopy proved valuable for non-invasive assessment of optical properties in muscles, enabled more comprehensive evaluation of hemiparetic muscles, and provided optimal monitoring of the effectiveness of medication.

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Photoacoustic Measurement and Experiment of Glucose Concentrations in an Aqueous Matrix

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.456.652 ◽

2013 ◽

Vol 456 ◽

pp. 652-655

Author(s):

Guo Dong Liu ◽

Jian Dong ◽

Zhong Ren ◽

Lv Ming Zeng ◽

Hao Xu

Keyword(s):

Blood Glucose ◽

Near Infrared ◽

Glucose Solution ◽

Generalized Least Squares ◽

Infrared Region ◽

Exponential Fitting ◽

Non Invasive ◽

Linear Polynomial ◽

Invasive Method ◽

Near Infrared Region

In the near-infrared region, we can test the blood glucose by non-invasive method. Based on this theory, combined photoacoustic principle, we had research about the simulated samples of blood glucose, measured the concentration of glucose solution. We have discussed the optimal wavelength in the near-infrared region (780-2400nm), and did the experiment repeatedly, and then analyzed data through the linear, polynomial and exponential fitting of the Generalized Least Squares, the measuring accuracy of the glucose solution had improved. These research results provide the guidance on the practice and experimental evidence for the further research of measuring.

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In-vivo near-infrared optical imaging of growing osteosarcoma cell lesions xenografted in mice: dual-channel quantitative evaluation of volume and mineralization

Acta Radiologica ◽

10.1258/ar.2011.110131 ◽

2011 ◽

Vol 52 (9) ◽

pp. 978-988 ◽

Cited By ~ 8

Author(s):

Hitoshi Nakayama ◽

Tomoyuki Kawase ◽

Kazuhiro Okuda ◽

Larry F Wolff ◽

Hiromasa Yoshie

Keyword(s):

Optical Imaging ◽

Near Infrared ◽

Imaging Technique ◽

Ex Vivo ◽

Acquisition Time ◽

Osteosarcoma Cell ◽

Dual Channel ◽

Nir Imaging ◽

Ex Vivo Imaging

Background In a previous study using a rodent osteosarcoma-grafted rat model, in which cell-dependent mineralization was previously demonstrated to proportionally increase with growth, we performed a quantitative analysis of mineral deposit formation using 99mTc-HMDP and found some weaknesses, such as longer acquisition time and narrower dynamic ranges (i.e. images easily saturated). The recently developed near-infrared (NIR) optical imaging technique is expected to non-invasively evaluate changes in living small animals in a quantitative manner. Purpose To test the feasibility of NIR imaging with a dual-channel system as a better alternative for bone scintigraphy by quantitatively evaluating mineralization along with the growth of osteosarcoma lesions in a mouse-xenograft model. Material and Methods The gross volume and mineralization of osteosarcoma lesions were evaluated in living mice simultaneously with dual-channels by NIR dye-labeled probes, 2-deoxyglucose (DG) and pamidronate (OS), respectively. To verify these quantitative data, retrieved osteosarcoma lesions were then subjected to ex-vivo imaging, weighing under wet conditions, microfocus-computed tomography (μCT) analysis, and histopathological examination. Results Because of less scattering and no anatomical overlapping, as generally shown, specific fluorescence signals targeted to the osteosarcoma lesions could be determined clearly by ex-vivo imaging. These data were well positively correlated with the in-vivo imaging data ( r > 0.8, P < 0.02). Other good to excellent correlations ( r > 0.8, P < 0.02) were observed between DG accumulation and tumor gross volume and between OS accumulation and mineralization volume. Conclusion This in-vivo NIR imaging technique using DG and OS is sensitive to the level to simultaneously detect and quantitatively evaluate the growth and mineralization occuring in this type of osteosarcoma lesions of living mice without either invasion or sacrifice. By possible mutual complementation, this dual imaging system might be useful for accurate diagnosis even in the presence of overlapping tissues.

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Design of an Ingestible Capsule With Wireless Communication and Powering Function for Obesity Treating

Volume 10: Micro- and Nano-Systems Engineering and Packaging ◽

10.1115/imece2013-63599 ◽

2013 ◽

Author(s):

Liang Yan ◽

Tianyi Wang ◽

Zongxia Jiao ◽

Juanjuan Peng

Keyword(s):

Morbid Obesity ◽

Wireless Communication ◽

In Vitro Experiments ◽

Non Invasive ◽

Removal Process ◽

Wireless Control ◽

Invasive Method ◽

Energy Shortage

Intra-gastric balloons have been and effective and non-invasive method for morbid obesity treating since it is proposed. However, traditional balloons lead to complications such as nausea and sickness caused by insertion and removal endoscopes. Despite free of endoscope-guide insertion and removal process, wireless controlled balloons still have to face the problem of energy shortage. This paper proposes a novel wireless controlled and powered endoscope capsule of edible size. The performance of wireless control and powering are tested respectively. In addition, in-vivo and in-vitro experiments are conducted for further evaluation and shows feasibility for treating morbid obesity. This study may contribute to the development of endoscopic devices and surgery as well.

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Oh, the places you’ll go!

Science Translational Medicine ◽

10.1126/scitranslmed.aar7522 ◽

2018 ◽

Vol 10 (425) ◽

pp. eaar7522

Author(s):

Jennifer A. Philips

Keyword(s):

Stem Cells ◽

Amyloid Plaques ◽

Selection Strategy ◽

In Vivo Selection ◽

Β Amyloid ◽

The Brain

An in vivo selection strategy identifies an antibody that induces stem cells to differentiate into microglia-like cells, migrate to the brain, and reduce β-amyloid plaques in mice.

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