Protective effect of polysaccharides from Radix Hedysari on gastric ulcers induced by acetic acid in rats

2019 ◽  
Vol 10 (7) ◽  
pp. 3965-3976 ◽  
Author(s):  
Zhiyuan Xue ◽  
Gengen Shi ◽  
Yaoyao Fang ◽  
Xiaohua Liu ◽  
Xianglin Zhou ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Gastric Ulcer ◽  
Protective Effect ◽  
Gastric Ulcers

The potential anti-gastric ulcer effects of Hedysarum polysaccharides (HPS-50 and HPS-80) were explored in rats.

scholarly journals Immunohistochemical Studies of Age-Related Changes in Cell Proliferation and Angiogenesis during the Healing of Acetic Acid-Induced Gastric Ulcers in Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
A. Folorunsho Ajayi ◽  
S. Babafemi Olaleye
Keyword(s):  
Cell Proliferation ◽  
Acetic Acid ◽  
Gastric Ulcer ◽  
Factor Viii ◽  
Ulcer Healing ◽  
Gastric Ulcers ◽  
Gastric Injury ◽  
Tissue Samples ◽  
Age Related ◽  
Male Wistar Rats

Cell proliferation and angiogenesis are of utmost importance for healing to take place. The KI67 and EGFR proteins are markers of cell proliferation, while CD31 and factor VIII are markers of angiogenesis. To elucidate the mechanism responsible for delayed healing of the gastric injury in old age, we analyzed the expression of these markers in rats of different months during the healing of an acetic acid-induced gastric ulcer. Male Wistar rats (aged 3, 6, 12, and 18 months) divided into four groups, according to their ages, formed the experimental animals. Stomach tissue samples were collected on days 3, 7, 14, and 21 after induction for assessment of ulcer healing. The area of gastric mucosa healed was inversely proportional to age. The expression of markers of proliferation (KI67 and EGFR) and angiogenesis (factor VIII and CD31) decreased significantly (p<0.05) in older rats when compared with younger ones (3 months > six months > 12 months > 18 months) on days 7, 14, and 21 after induction of gastric ulcer. This study revealed that the slower gastric ulcer healing rate in older rats might be due to reduced epithelial cell proliferation and angiogenic activities.

scholarly journals ANTIOXIDANT AND ANTIULCER ACTIVITY OF ABRUS PRECATORIUS LEAF EXTRACT AGAINST ACETIC ACID-INDUCED GASTRIC ULCER IN RATS

2019 ◽  
pp. 218-220
Author(s):  
LALITA NAGDA ◽  
CHANDRA PRAKASH JOSHI ◽  
JYOTI MENARIA
Keyword(s):  
Acetic Acid ◽  
Gastric Ulcer ◽  
Leaf Extract ◽  
Defense Mechanism ◽  
Gastric Volume ◽  
Gastric Ulcers ◽  
Antiulcer Activity ◽  
Plant Leaves ◽  
Once Daily ◽  
Abrus Precatorius

Objective: The present study deals with the study of antiulcer activity of Abrus precatorius leaf extract in acetic acid-induced gastric ulcers was studied in rats. Methods: In the present study, A. precatorius leaf extract was carried out in antiulcer activity against acetic acid-induced gastric ulcer in rats. A. precatorius at doses of 150 mg/kg and 300 mg/kg were administered orally once daily for 10 days. Results: It was found that extract significantly reduced ulcer index. The plant leaves made gastric anti-secretory effect by decreasing gastric volume and acidity. Then, gastric mucin was increased, which showed gastric cytoprotective effect. The plant showed the prevention effect of increased lipid peroxidation during ulceration by acetic acid. Activities of the antioxidant enzymes were enhanced during ulceration by this plant leaves. Conclusion: Leaves of A. precatorius Linn. could decrease acetic acid-induced gastric ulcers in rats and this antiulcerogenic activity was mediated through antioxidant defense mechanism.

scholarly journals Evaluation of Antiulcerogenic Activity of Clerodendron Infortunatum Extract on Albino Rat Gastric Ulceration

2019 ◽  
Vol 9 (4-A) ◽  
pp. 57-62
Author(s):  
Arun Kumar ◽  
Rahul Shukla ◽  
Anurag Chaudhary
Keyword(s):  
Acetic Acid ◽  
Gastric Ulcer ◽  
Restraint Stress ◽  
Ethanolic Extract ◽  
North India ◽  
Gastric Ulcers ◽  
Albino Rat ◽  
Pylorus Ligation ◽  
Cold Restraint Stress ◽  
Cold Restraint

Clerodendron infortunatum Linn. (Verbenaceae) is an important and widely used medicinal plant, reported to contain active bitter substance like clerodin, has been widely used as tonic and anthelmintic agent in the country sides of North India. Though, variously used in Ayurveda, Unani system of medicine and Homeopathy in case of ailments like diarrhoea, skin disorders, venereal and scrofulous complaints, wounds, post-natal complications, as vermifuge, laxative and cholagogue, for the removal of ascarids in anus, as external applications on tumours, etc., Background: The present study reports the antiulcer properties of 50% ethanolic extract of Clerodendron infortunatum Linn. (Verbenaceae) leaves have assessed in different acute and chronic gastric ulcer models in rats. Methods: EECI (100, 200 and 400 mg/kg body weight) was administered orally, twice daily for 5 days for prevention from ethanol (EtOH), Cold-restraint stress (CRS), Pylorus-ligation (PL) and 10 days for prevention of acetic acid induced ulcers. Results: The EECI showed significant gastric ulcer protective effect in doses of 400 mg/kg, when given twice daily for 5 days against gastric ulcers induced by ethanol (EtOH), cold restraint stress (CRS) and pyloric ligation (PL). EECI showed dose dependent decrease in ulcer index (UI) against ulcers induced by: (i) Ethanol (control UI: 29.52±2.4 mm2/rat, EECI decrease 21.12±2.3 – 5.3±1.0 (ii) Cold restraint stress (CRS) (control UI: 32.13±2.1, EECI decrease 24.02±2.2 – 4.7±1.1 (iii) pylorus ligation (control UI: 29.25±2.4 mm2/rat, EECI decrease 22.32±2.7 – 5.3±1.0, EECI 400 mg/kg significantly healed ulcers induced by 50% acetic acid after 5 (control UI: 32.30 ± 2.9, EECI decrease 26.27±1.9 – 8.56±0.98 and after 10 days treatment (control UI: 29.02 ± 2.6, healing 21.28±2.1 – 5.78±1.2. EECI prevents the oxidative damage of gastric mucosa by blocking lipid peroxidation and by significant decrease in superoxide dismutase, and increase in catalase activity. Conclusions: Our results show that Clerodendron infortunatum Linn. (Verbenaceae)  possess significant gastro-protective activity which might be due to gastric defence factors and clerodin might be the main constituents responsible for this activity.

scholarly journals Gastroprotective Effects of Methanolic Leaves and Stem Extracts of Sphagneticola trilobata on Indomethacin-Induced Gastric Ulcer in Rats

2021 ◽  
Vol 34 (1) ◽  
pp. 111-117
Author(s):  
Kartik Singhal ◽  
Chandana Majee ◽  
Bhavani Pentela ◽  
Vikas Sharma
Keyword(s):  
Gastric Ulcer ◽  
South America ◽  
Medicinal Plant ◽  
Protective Effect ◽  
Folk Medicine ◽  
Gastric Ulcers ◽  
Control Group ◽  
Methanolic Extracts ◽  
Neutrophil Percentage ◽  
Stem Extract

The medicinal plant Sphagneticola trilobata native to South America is used in local folk medicine to treat inflammation and analgesics. In present study, gastroprotective effects of methanolic extracts of S. trilobata leaves and stem were investigated in indomethacin-induced gastric ulcers in rats at doses of 200 mg/kg for leave extract and 350 mg/kg for stem extract. Indomethacin produced stomach ulcers and increased neutrophil percentage and MDA levels compared to the control group (p < 0.001). Co-administration of indomethacin and omeprazole, methanolic extracts of leaves (200 mg/kg) (p < 0.001) and methanolic extracts (350 mg/kg) (p < 0.05) of stem compared to indomethacin group to ulcers was low (p < 0.001). Methanolic extracts (200 mg/kg) of leaves and methanolic extracts (350 mg/kg) of stem reduced MDA levels (p < 0.001). Methanolic extracts (200 mg/kg) of leaves and methanolic extracts (350 mg/kg) of the stem significantly decreased neutrophil percentage compared to indomethacin group (p < 0.001). The results suggest that the methanolic extracts of Sphagneticola trilobata leaves and stem have a protective effect on indomethacin-induced gastric ulcers.

Protective Effect of Cod (Gadus macrocephalus) Skin Collagen Peptides on Acetic Acid-Induced Gastric Ulcer in Rats

2016 ◽  
Vol 81 (7) ◽  
pp. H1807-H1815 ◽  
Author(s):  
Huina Niu ◽  
Zhicong Wang ◽  
Hu Hou ◽  
Zhaohui Zhang ◽  
Bafang Li
Keyword(s):  
Acetic Acid ◽  
Gastric Ulcer ◽  
Protective Effect ◽  
Collagen Peptides ◽  
Gadus Macrocephalus ◽  
Skin Collagen

scholarly journals Evaluation of Therapeutic Efficacy of Stigma and Petal Extracts of Crocus sativus L. on Acetic Acid-Induced Gastric Ulcer in Rats

2021 ◽  
Author(s):  
Mahtab Mohammadifard ◽  
Hossein Javdani ◽  
Ghazaleh Khalili-Tanha ◽  
Ali Farahi ◽  
Mohsen Foadoddini ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Gastric Ulcer ◽  
Healing Process ◽  
Crocus Sativus ◽  
Gastric Ulcers ◽  
Control Group ◽  
Dependent Manner ◽  
Ulcer Size ◽  
Ethanolic Extracts ◽  
Crocus Sativus L

Saffron (Crocus sativus L.) has long been considered a medicinal plant in Traditional Persian Medicine (TPM) due to its therapeutic properties. Despite this interest, its effects on gastrointestinal disorders have not been completely taken into consideration. Hence, this study aimed to evaluate the pharmacological activity of ethanolic extracts of saffron stigma (SS) and saffron petal (SP) in acetic acid-induced gastric ulcer in rats. The gastric ulcer model was imitated by the serosal application of acetic acid in male Wistar rats. Then, the animals were orally fed with 100 mg/kg and 200 mg/kg of ethanolic extracts of SS or SP, omeprazole (40 mg/kg), or saline for 12 days. The macroscopic and microscopic appearances of gastric ulcers and the levels of malondialdehyde (MDA), vascular endothelial growth factor (VEGF), and prostaglandin E2 (PGE2) in gastric tissues were assessed. The highest anti-ulcer activity was observed in the omeprazole-treated animals with the lowest ulcer size (4.29 ± 1.78 mm2). SS could not reduce gastric ulcer size in rats. Compared to the untreated rats, SP treatment significantly decreased ulcer indices in a dose-dependent manner. The gastric levels of PGE2, VEGF, and MDA were significantly elevated in the untreated animals with gastric ulcers compared to rats in the control group. The SS extract suppressed the elevated PGE2 and VEGF levels at both doses, while SP did not have a significant influence. Both SS and SP treatments significantly ameliorated MDA levels in rats with gastric ulcers. Omeprazole treatment enhanced the PGE2 level and suppressed MDA contents, but it did not influence the VEGF level. In conclusion, our findings demonstrated that the saffron stigma has no significant effects on the gastric ulcer healing process, while its petals accelerate the process. This discrepancy can be attributed to the difference in the main secondary metabolites between saffron stigma and petals.

scholarly journals Chrysin Modulates Genes Related to Inflammation, Tissue Remodeling, and Cell Proliferation in the Gastric Ulcer Healing

2020 ◽  
Vol 21 (3) ◽  
pp. 760 ◽  
Author(s):  
Felipe Leonardo Fagundes ◽  
Graziele de Morais Piffer ◽  
Larissa Lucena Périco ◽  
Vinicius Peixoto Rodrigues ◽  
Clélia Akiko Hiruma-Lima ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Acetic Acid ◽  
Gastric Ulcer ◽  
Gastric Mucosa ◽  
Caspase 3 ◽  
Ischemia Reperfusion ◽  
Absolute Ethanol ◽  
Gastric Ulcers ◽  
Healing Effect ◽  
Cox 2

Chrysin exhibits anti-inflammatory and antioxidant activities. Here, the gastroprotective effect of chrysin was investigated in mouse models of gastric ulcer induced by absolute ethanol, acetic acid, and ischemia-reperfusion injury. The gastric-healing effect was evaluated at 7 and 14 days after treatment; the mechanism of action was verified using the expression of metalloproteinase 2 (MMP-2) and 9 (MMP-9), caspase-3, cyclooxygenase 1 (COX-1) and 2 (COX-2), epidermal growth factor (EGF), and interleukin-10. Chrysin (10 mg/kg) inhibited macroscopic lesions and increased catalase activity in the mouse model established using absolute ethanol. It ameliorated the gastric ulcer caused by acetic acid by improving the expression of inflammatory genes such as COX-2, inhibiting negative remodeling promoted by MMP-9, increasing cell proliferation effect via EGF, and reducing cellular apoptosis by modulating caspase-3. A faster healing effect was evident in the first 7 days of treatment compared to 14 days of treatment, indicating the pharmacological potential of chrysin. Overall, these results demonstrate the potent effect of chrysin in the gastrointestinal tract and elucidate the genes involved in the healing of gastric ulcers. Moreover, an increase in the levels of gastric mucosa defensive factors is involved in the activity of chrysin in the gastric mucosa.

scholarly journals The Gastro Protective Effect of Trigonella Foenum Graecum Seed (Methi) and Omeprazole in Experimentally Induced Gastric illcer in Rats

2018 ◽  
Vol 26 (2) ◽  
pp. 126-131
Author(s):  
Rumana Afroz ◽  
Kazi Afzalur Rahman ◽  
AHM Mostafa Kamal ◽  
Mahbuba Jahan Lotus ◽  
Sultana Yesmin ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Protective Effect ◽  
Aqueous Extract ◽  
Developed Countries ◽  
Gastric Epithelium ◽  
Gastric Ulcers ◽  
Lesion Length ◽  
Medical College ◽  
Trigonella Foenum Graecum ◽  
Significant Difference

Context : The gastric ulcer is a common gastrointestinal problem in Bangladesh. Gastric epithelium is under constant assault by a series of endogenous noxious factors, including HCl, pepsinogen / pepsin, and bile salts. In addition, a steady flow of exogenous substances such as medications, alcohol, and bacteria encounter the gastric mucosa. A highly intricate biologic system is in place to provide defense from mucosal injury and to repair any injury that may occur. From the ancient time, various plants were used in traditional medicine with reputation as efficacious remedies. The incidence of duodenal ulcer disease has been declining dramatically for the past 30 years, but the incidence of gastric ulcers appears to be increasing as a result of the widespread use of NSAIDs and low-dose aspirin. The list of plant derived modern medicine is very long now. About 33% of the drugs produced in the developed countries are derived from plants. Trigonella foenumgraecum (Fenugreek, Methi) is a herbal medicine used in many parts of world. Preliminary study on animal showed that Trigonella foenum-graecum seed has significant gastro-protective effect. A study was carried out to see the gastro-protective effect of Trigonella foenum-graecum (Fenugreek, Methi) and omeprazole in ethanol induced gastric ulcer in an experimental rats.Material and Methods: The present study was performed on 24 (twenty four) rats which were divided randomly into 4 groups each having 6 rats in the Pharmacology Department of Dhaka Medical college, Dhaka. The rats were aged between 8-10 weeks of both sexes and weighing between 180-200 gm. The study was carried out to demonstrate the gastro-protective effect of aqueous extract and ethanolic extract of Trigonella foenum-graecum seed and omeprazole on ethanol induced gastric ulcer in experimental rats.Result : Pretreatment with aqueous extract and ethanolic of Trigonella foenum-graecum seed (500mg/kg/body wt) showed very significant prevention in ethanol induced gastric ulcer in number of lesion, lesion length, lesion breadth, lesion area and lesion index. This prevention was statistically very significant (P<0.001). In this study there was no statistical significant difference was observed between aqueous and ethanolic extracts of Trigonella foenumgraecum seed in gastro-protective effects.Conclusion: The aqueous and ethanol extracts of Trigonella foenum-graecum seed and omeprazole possess gastro protective properties.J Dhaka Medical College, Vol. 26, No.2, October, 2017, Page 126-131

scholarly journals Analgesic, anti-inflammatory and anti-ulcer properties of Thai Perilla frutescence fruit oil in animals

2021 ◽  
Author(s):  
Narisara Paradee ◽  
Pimpisid Koonyosying ◽  
Winthana Kusirisin ◽  
Rattanaporn Janthip ◽  
Duangta Kanjanapothi ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Gastric Ulcer ◽  
Inflammatory Diseases ◽  
Biological Activities ◽  
Water Immersion ◽  
Gastric Ulcers ◽  
Paw Edema ◽  
Ear Edema ◽  
Anti Inflammatory ◽  
Fruit Oil

Perilla frutescens fruits (PFO) is rich in α-linolenic acid (ALA) and exhibits biological activities. We aimed to investigate analgesic, anti-inflammatory and anti-ulcer activities of PFO and PFO-supplemented soybean milk (PFO-SM) in animal models. Analgesic activity was assessed in acetic acid-induced writhing in mice, while anti-inflammatory activity was performed in ethyl phenylpropiolate (EPP)-induced ear edema and carrageenan-induced hind paw edema in rats. Anti-ulcer effects were conducted in water immersion stress, HCl/ethanol, and indomethacin-induced gastric ulcer in rats. Distinctly, PFO (6.96 mg ALA and 2.61 LA mg equivalent/g) did not induce acute toxicity (LD50 &gt;10 mL/kg) in mice. PFO (2.5 and 5 mL/kg) and PFO-SM (0.05 mL PFO equivalence/kg) inhibited incidences of writhing (16.8, 18 and 32.3%, respectively) in acetic acid-induced mice. In addition, topical applications of PFO (0.1 and 1 mL/ear) significantly inhibited EPP-induced ear edema (59.3 and 65.7%, respectively) in rats, while PFO-SM slightly inhibited ear edema (25.9%). However, PFO and PFO-SM did not inhibit carrageenan-induced hind paw edema in rats. Indeed, PFO (2.5 and 5 mL/kg) significantly inhibited gastric ulcers in rats that induced by water immersion stress (92.4 and 96.6%, respectively), HCl/ethanol (74.8 and 73.3%, respectively) and indomethacin (68.8 and 88.9%, respectively), while PFO-SM did not. PFO displayed potent analgesic, anti-inflammatory and anti-ulcer properties, while PFO-SM exerted only analgesic properties. Thus, Thai perilla fruit oil and its functional drink offers potential benefits in treatment of analgesic, inflammatory diseases and gastric ulcer.

scholarly journals Role of Daucus carota in Enhancing Antiulcer Profile of Pantoprazole in Experimental Animals

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5287
Author(s):  
Syed Mohammed Basheeruddin Asdaq ◽  
Earla Swathi ◽  
Sunil S Dhamanigi ◽  
Mohammed Asad ◽  
Yahya Ali Mohzari ◽  
...  
Keyword(s):  
Duodenal Ulcer ◽  
Acetic Acid ◽  
Gastric Ulcer ◽  
Daucus Carota ◽  
Ulcer Healing ◽  
Gastric Ulcers ◽  
Chronic Gastric Ulcer ◽  
Experimental Animals ◽  
Pylorus Ligation

The carrot plant (Daucus carota) and its components are traditionally reported for the management of gastric ulcers. This study was performed to evaluate the role of carrot when administered concurrently with a conventional antiulcer treatment, pantoprazole, in alleviating gastric and duodenal ulcers in female experimental animals. The study involved standard animal models to determine the ulcer preventive effect using pylorus ligation, ethanol, and stress induced acute gastric ulcer models and duodenal ulcer models involving cysteamine. Acetic acid-induced chronic gastric ulcer and indomethacin-induced gastric ulcer models were used to evaluate the ulcer healing effect. Carrot fruit (500 mg/kg) and its co-administration with pantoprazole produced significant protection in an ethanol- and stress-induced acute gastric ulcer and cysteamine-induced duodenal ulcer. The healing of the acetic acid-induced chronic gastric ulcer was also augmented with this combination. Both total proteins and mucin contents were significantly increased in indomethacin-induced gastric ulcers. Similarly, in pylorus ligation, the pepsin content of gastric juice, total acidity, and free acidity were reduced. Overall, both ulcer preventive effects and ulcer healing properties of the pantoprazole were significantly enhanced in animals who received the co-administration of carrot fruit (500 mg/kg).

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