A thermodynamic and kinetic study of the antioxidant activity of natural hydroanthraquinones

Novel hydroanthraquinones isolated from Talaromyces islandicus EN-501 exhibited promising antioxidant properties in preliminary studies, raising the prospect of adapting these compounds for therapeutic use in diseases caused by oxidative stress.

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In Vitro Study of Antioxidant Activity of Serum and Plasma Samples As Well As Glutathione Exposed to Various Exogenous Stress Factors

10.21203/rs.3.rs-630921/v1 ◽

2021 ◽

Author(s):

Małgorzata Olszowy-Tomczyk ◽

Łukasz Paprotny ◽

Agnieszka Celejewska ◽

Dorota Szewczak ◽

Dorota Wianowska

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Antioxidant Properties ◽

In Vitro Study ◽

Stress Factors ◽

Plasma Samples ◽

Serum Samples ◽

Exogenous Stress

Abstract The imbalance between the production of Reactive Oxygen Species (ROS) and their sequestration promotes the formation of so-called oxidative stress conditions which are considered crucial in the aging process and development of many human diseases. Glutathione plays an essential role in the antioxidative barricade against ROS. Its role in the detoxification process of xenobiotics and carcinogen is also known. However, there are no comparative studies on the antioxidant properties of both biological samples and glutathione as well as the change in these properties as a result of exposure to various stress factors. This paper fills this gap comparing the antioxidant activity of serum and plasma samples of the known glutathione content with the activity of glutathione itself assessed by the different methods. In addition, it reveals a significant role of environmental xenobiotics in oxidative stress and differentiates the stress induced by different groups of drugs, among which the greatest one has been demonstrated for antiarrhythmic drugs and cytostatics. More importantly, it proves that human plasma is more resistant to stress factors and N-acetylcysteine clearly promotes the extension of antioxidant properties of both the plasma and serum samples. The latter conclusion is consistent with the implied preventive and/or supportive action of this drug against SARS-CoV-2.

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Acrocomia aculeata (Jacq.) Lodd. ex Mart. Leaves Increase SIRT1 Levels and Improve Stress Resistance

Oxidative Medicine and Cellular Longevity ◽

10.1155/2020/5238650 ◽

2020 ◽

Vol 2020 ◽

pp. 1-16

Author(s):

Tamaeh Monteiro-Alfredo ◽

Paulo Matafome ◽

Bianca Pancoti Iacia ◽

Kátia Ávila Antunes ◽

Jéssica Maurino dos Santos ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Experimental Models ◽

Ros Generation ◽

Leaf Extracts ◽

C Elegans ◽

Acrocomia Aculeata

Oxidative stress is a metabolic disorder linked with several chronic diseases, and this condition can be improved by natural antioxidants. The fruit pulp of the palm Acrocomia aculeata (Jacq.) Lodd. ex Mart. is widely used in the treatment of various illnesses, but as far as we know, there are no reports regarding the properties of its leaves. Thus, we aimed to evaluate the antioxidant activity of A. aculeata leaf extracts obtained with water (EA-Aa), ethanol (EE-Aa), and methanol (EM-Aa) solvents. The extracts were chemically characterized, and their antioxidant activity was assessed through the scavenging of the free radicals DPPH and ABTS. EE-Aa and EM-Aa showed the highest amounts of phenolic compounds and free radical scavenging activity. However, EA-Aa was more efficient to protect human erythrocytes against AAPH-induced hemolysis and lipid peroxidation. Thus, we further show the antioxidant effect of EA-Aa in preventing AAPH-induced protein oxidation, H2O2-induced DNA fragmentation, and ROS generation in Cos-7 cells. Increased levels of Sirt1, catalase, and activation of ERK and Nrf2 were observed in Cos-7 treated with EA-Aa. We also verify increased survival in nematodes C. elegans, when induced to the oxidative condition by Juglone. Therefore, our results showed a typical chemical composition of plants for all extracts, but the diversity of compounds presented in EA-Aa is involved in the lower toxicity and antioxidant properties provided to the macromolecules tested, proteins, DNA, and lipids. This protective effect also proven in Cos-7 and in C. elegans was probably due to the activation of the Sirt1/Nrf2 pathway. Altogether, the low toxicity and the antioxidant properties of EA-Aa showed in all the experimental models support its further use in the treatment of oxidative stress-related diseases.

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Antioxidant activity of ethanolic extract of three Selaginella species from Java Island, Indonesia

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d201234 ◽

2019 ◽

Vol 20 (12) ◽

Author(s):

M Miftahudin ◽

Rini Hasibuan ◽

Tatik Chikmawati

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Antioxidant Activity ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Sod Activity ◽

Different Doses

Abstract. Miftahudin, Hasibuan RS, Chikmawati T. 2019. Antioxidant activity of ethanolic extract of three Selaginella species from Java Island, Indonesia. Biodiversitas 20: 3715-3722. Three Selaginella species, S. ornata, S. plana, and S. willdenowii, from Java Island, Indonesia, have been known to have antioxidant properties; however, in vivo antioxidant activities of these species have not been reported. This research aimed to evaluate the in vivo antioxidant activity of ethanolic extract of three Selaginella species. The 70% ethanol extract of three Selaginella species at four different doses was administered to mice one day before being treated with oxidative stress. The liver tissue of mice treated with or without oxidative stress was analyzed their lipid peroxidation by measuring MDA concentration and Superoxide Dismutase (SOD) activities. The results showed that there were variations in antioxidant activity among the three Selaginella species. In general, the dose of 0.3 g extract kg-1 BW has been able to reduce lipid peroxidation and increase SOD activity. The administration of S. ornata extract to the mice at 1.2 g extract kg-1 BW reduced the MDA concentration to the lowest level, but the same dose of two other Selaginella extracts caused toxic effects in mice. The antioxidant activities of S. ornata and S. plana were better than that of S. willdenowii extract, and among those species, S. ornata has the best antioxidant activity.

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The Chemical Profile ofSenna velutinaLeaves and Their Antioxidant and Cytotoxic Effects

Oxidative Medicine and Cellular Longevity ◽

10.1155/2016/8405957 ◽

2016 ◽

Vol 2016 ◽

pp. 1-12 ◽

Cited By ~ 18

Author(s):

Jaqueline Ferreira Campos ◽

David Tsuyoshi Hiramatsu de Castro ◽

Marcio José Damião ◽

Heron F. Vieira Torquato ◽

Edgar J. Paredes-Gamero ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Leukemic Cell ◽

Ethanol Extract ◽

Human Erythrocytes ◽

Leukemic Cells ◽

Cytotoxic Activities ◽

Leukemic Cell Lines

Natural products can be a source of biomolecules with antioxidant activity which are able to prevent oxidative stress-induced diseases and show antitumor activity, making them important sources of new anticancer drug prototypes. In this context, this study aimed to analyze the chemical composition of an ethanol extract ofSenna velutinaleaves and to assess its antioxidant and cytotoxic activities in leukemic cells. The antioxidant properties were evaluated using a DPPH free radical scavenging assay and by examining the extract’s inhibition of AAPH-induced lipid peroxidation in human erythrocytes. Its cytotoxicity and possible mechanisms of action were assessed in Jurkat and K562 leukemic cell lines. The ethanol extract contained flavonoids, such as epigallocatechin, epicatechin, kaempferol heteroside, rutin, and dimeric and trimeric proanthocyanidin derivatives. The extract exhibited antioxidant activity by scavenging free radicals and antihemolytic action, and it decreased malondialdehyde content in human erythrocytes. Furthermore, the extract also induced leukemic cell death by activating intracellular calcium and caspase-3, decreasing mitochondrial membrane potential, and arresting the cell cycle in S and G2 phases. Hence,S. velutinaleaf extract contains antioxidant and antileukemic biomolecules with potential applications in diseases associated with oxidative stress and in the inhibition of tumor cell proliferation.

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Culinary–medicinal mushrooms: a review of organic compounds and bioelements with antioxidant activity

European Food Research and Technology ◽

10.1007/s00217-020-03646-1 ◽

2020 ◽

Author(s):

Adrian Podkowa ◽

Agata Kryczyk-Poprawa ◽

Włodzimierz Opoka ◽

Bożena Muszyńska

Keyword(s):

Oxidative Stress ◽

Central Nervous System ◽

Antioxidant Activity ◽

Antioxidant Properties ◽

Cardiovascular Disorders ◽

Indole Derivatives ◽

Medicinal Mushrooms ◽

Digestive Juice ◽

The Central Nervous System ◽

Artificial Digestive Juice

AbstractThere are about 3000 species of mushrooms, which have a high amount of substances that are beneficial to human health, such as antioxidants. It is well known that oxidative stress plays an important role in the etiopathogenesis of many diseases, including cancer, cardiovascular disorders, and diseases of the central nervous system. One way to prevent homeostasis disorders that occur as a result of excessive production of pro-oxidative substances is to include the ingredients having antioxidant properties in the diet. Several compounds, such as those with phenolic and indole derivatives as well as carotenoids and some vitamins, exhibit antioxidant activity. These substances are present in many foods, including mushrooms. In addition, they have certain unique compounds that are not found in other sources (e.g., norbadione A). The present work discusses selected ingredients exhibiting antioxidant activity, which are found in various species of mushrooms as wells as describes the content of these compounds in the extracts obtained from mushrooms using artificial digestive juice.

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Comparison of Ethanolic and Aqueous-Polyethylenglycolic Propolis Extracts: Chemical Composition and Antioxidant Properties

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2021/5557667 ◽

2021 ◽

Vol 2021 ◽

pp. 1-7

Author(s):

Mindaugas Liaudanskas ◽

Loreta Kubilienė ◽

Vaidotas Žvikas ◽

Sonata Trumbeckaitė

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Chemical Composition ◽

Phenolic Acids ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Natural Antioxidants ◽

Alternative Form ◽

Food Preservatives ◽

Propolis Extract

In recent years, particular attention has been paid to the natural antioxidants. Bee products, especially propolis, are characterized by multifunctional (antioxidant, anti-inflammatory, antibacterial, antiviral, and food preservative) effects and can be used for the development of functional food or food preservatives. Propolis extracts that are commonly produced are ethanolic; therefore, to certain groups of consumers, for example, children and alcohol sensitive group, their applicability is limited. The aim of this study was to develop alternative propolis from aqueous-polyethylenglycolic propolis extract (AQUA-PEG) and compare the chemical composition as well as antioxidant (radical-scavenging and reduction properties) activities to those of ethanolic propolis extract (EEP). Polyethylene glycol is quite a good solvent, which can be successfully used for the preparation of NEP. The total quantity of phenolic compounds identified in AQUA-PEG (400.36 µg/mL), prepared according to our technology, is very similar to that of EEP (433.53 µg/mL), whereas the amount of phenolic acids was greater by 1.31-fold in AQUA-PEG and of flavonoids was greater by 2.38-fold in EEP. The antioxidant activity depends on the method used: by applying the ABTS and CUPRAC methods, both extracts demonstrate similar antioxidant (antiradical and reducing) activity, whereas in the case of the DPPH and FRAP method, significantly higher antioxidant activity was detected in EEP. This should be taken by researchers into account especially when interpreting the results and drawing conclusions about the antioxidant activity of propolis extracts. On the basis of the results, AQUA-PEG, prepared by the developed technology, can be used as an alternative form to ethanolic propolis extract, since it contains a large quantity of antioxidants, namely, flavonoids and phenolic acids. We believe that nonethanolic propolis extract has the prospect of being applied for the development of functional foods in order to alleviate certain symptoms of oxidative stress or for the prevention of some oxidative-stress-related diseases.

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Coffee Infusions: Can They Be a Source of Microelements with Antioxidant Properties?

Antioxidants ◽

10.3390/antiox10111709 ◽

2021 ◽

Vol 10 (11) ◽

pp. 1709

Author(s):

Ewa Olechno ◽

Anna Puścion-Jakubik ◽

Katarzyna Socha ◽

Małgorzata Elżbieta Zujko

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Health Effect ◽

Antioxidant Properties ◽

Daily Intake ◽

Google Scholar ◽

Manganese Zinc ◽

Coffee Beans ◽

The World ◽

Brewing Method

Coffee is a beverage that is very popular all over the world. Its pro-health effect has been demonstrated in many publications. This drink can counteract the effects of oxidative stress thanks to its antioxidant properties. The aim of this study was to collect data on the content of microelements with antioxidant activity (manganese, zinc, copper, iron) in coffee infusions, taking into account various factors. The study considered publications from the years 2000–2020 found in Google Scholar and PubMed databases. It was noted that coffee can provide up to 13.7% of manganese requirements per serving, up to 4.0% and 3.1% of zinc requirements for women and men, up to 2.7% and 2.1% of copper requirements for women and men, and up to 0.4% and 0.6% of iron requirements for women and men. Coffee infusions can also be a source of fluoride (up to 2.5%), chromium (up to 0.4% of daily intake for women and 0.2% for men), and cobalt (up to 0.1%). There are no data in the literature regarding the content of selenium in coffee infusions. The origin of coffee beans and the type of water used (especially regarding fluoride) may have an impact on the content of minerals in infusions. The brewing method does not seem to play an important role. As it is a very popular beverage, coffee can additionally enrich the diet with such micronutrients as manganese, zinc, and copper. This seems beneficial due to their antioxidant properties, however the bioavailability of these elements of coffee should be taken into account. It seems necessary to carry out more research in this area.

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Antioxidant Properties of a Supercritical Fluid Extract of the Halophyte Mesembryanthemum nodiflorum L. from Sicilian Coasts: Nutraceutical and Cosmeceutical Applications

Applied Sciences ◽

10.3390/app10072374 ◽

2020 ◽

Vol 10 (7) ◽

pp. 2374 ◽

Cited By ~ 1

Author(s):

Rosaria Arena ◽

Simona Manuguerra ◽

Edward Collins ◽

Abdelkarim Mahdhi ◽

Giuseppe Renda ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Supercritical Fluid ◽

Antioxidant Properties ◽

Reducing Power ◽

Negative Effects ◽

Cell Vitality ◽

Aerial Parts ◽

Bioactive Properties

The aim of this paper was to obtain different extracts from the aerial parts of Mesembryanthemum nodiflorum, comparing traditional extraction (using solvents with increasing polarity such as hexane, ethanol 80%, acetone 70% and water) with an eco-friendly technique (supercritical fluid extraction (SFE)); to evaluate which extract showed a higher amount of antioxidants and then evaluate the bioactive properties in vitro, in human fibroblast (HS68). From the six extracts obtained it was observed that the solvent with the highest extraction efficiency was water, but the extracts in ethanol, N-hexane and SFE are those that showed the highest antioxidant activity (polyphenols, 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and reducing power). On the basis of these results, the SFE extract was chosen to evaluate the antioxidant and anti-aging activity in vitro, by assessing cell vitality and molecular markers (MTT and immunoblotting assays). The results showed that the SFE extract exerted antioxidant activity in vitro, protecting cells from mortality induced by oxidative stress; this protection was also confirmed at the molecular level, by the levels of the protein integrin -1, that is able to prevent the negative effects of a stress situation, such as oxidative stress, that could promote aging and related diseases. This extract, obtained with an eco-friendly technique, given its beneficial properties, could be used for application in nutraceuticals and cosmeceuticals.

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A Study on the Quality and Identity of Brazilian Pampa Biome Honey: Evidences for Its Beneficial Effects against Oxidative Stress and Hyperglycemia

International Journal of Food Science ◽

10.1155/2014/470214 ◽

2014 ◽

Vol 2014 ◽

pp. 1-11 ◽

Cited By ~ 11

Author(s):

L. C. Cruz ◽

J. E. S. Batista ◽

A. P. P. Zemolin ◽

M. E. M. Nunes ◽

D. B. Lippert ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Antioxidant Properties ◽

High Concentration ◽

Beneficial Effects ◽

Pampa Biome ◽

First Time

We characterized, for the first time, the quality and identity of Brazilian Pampa biome honey and its antioxidant propertiesin vitro(FRAP, DDPH and ABTS). The potential protective effect of honey against oxidative stress induced by iron (Fe) and paraquat, (PQ) in aDrosophila melanogastermodel (in vivo) was also tested. The results indicated that all honey samples tested showed antioxidant activityin vitro. Flies treated with honey showed increased lifespan and were protected against oxidative stress induced by Fe and PQ. Despite the high concentration of sugars in honey (approximately 70–80%), our results demonstrate a hypoglycemic-like effect of honey inDrosophila. Thus, this study demonstrates the high quality of Brazilian Pampa biome honey as well as its significant antioxidant activityin vitroandin vivo, pointing to the potential use of this natural product as an alternative in the therapy of oxidative stress-associated diseases.

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Preparation, Antioxidant Properties and Ability to Increase Intracellular NO of a New Pyridoxine Derivative B6NO

Antioxidants ◽

10.3390/antiox10091451 ◽

2021 ◽

Vol 10 (9) ◽

pp. 1451

Author(s):

Anastasia Balakina ◽

Tatyana Prikhodchenko ◽

Vera Amozova ◽

Tatyana Stupina ◽

Victoria Mumyatova ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Antioxidant Properties ◽

Nitrogen Monoxide ◽

Ros Generation ◽

Hybrid Compound ◽

No Donor ◽

Normal Cells ◽

Butyl Peroxide ◽

Low Cytotoxicity

In the case of various pathologies, an imbalance between ROS generation and the endogenous AOS can be observed, which leads to excessive ROS accumulation, intensification of LPO processes, and oxidative stress. For the prevention of diseases associated with oxidative stress, drugs with antioxidant activity can be used. The cytotoxic, antioxidant, and NO-donor properties of the new hybrid compound B6NO (di(3-hydroxy-4,5-bis(hydroxymethyl)-2-methylpyridinium) salt of 2-(nitrooxy)butanedioic acid) were studied. It was determined that B6NO chelates iron ions by 94%, which indicates B6NO’s ability to block the Fenton reaction. The hybrid compound B6NO inhibits the process of initiated lipid peroxidation more effectively than pyridoxine. It was shown that B6NO exhibits antioxidant properties by decreasing ROS concentration in normal cells during the oxidative stress induction by tert-Butyl peroxide. At the same time, the B6NO antioxidant activity on tumor cells was significantly lower. B6NO significantly increases the intracellular nitrogen monoxide accumulation and showed low cytotoxicity for normal cells (IC50 > 4 mM). Thus, the results indicate a high potential of the B6NO as an antioxidant compound.

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