scholarly journals Interactions of soya-bean agglutinin with purified glycoconjugates and soya-bean seed components

1985 ◽  
Vol 228 (1) ◽  
pp. 127-136 ◽  
Author(s):  
H M Bond ◽  
M F Chaplin ◽  
D J Bowles
Keyword(s):  
Particulate Matter ◽  
Trichloroacetic Acid ◽  
Lectin Binding ◽  
Soya Bean ◽  
Bean Seed ◽  
Wide Range ◽  
Triton X ◽  
Differential Solubility

A radioaffinity assay for lectin binding to receptors was developed and characterized by using the interactions between soya-bean agglutinin and four glycoconjugates, namely thyroglobulin, galactomannan, fetuin and asialofetuin. On application of the assay to soya-bean extracts a wide range of seed components were found to have the capacity to interact with soya-bean agglutinin. These included both trichloroacetic acid-soluble and trichloroacetic acid-insoluble glycoconjugates and two classes of particulate matter distinguished by their differential solubility in Triton X-100.

Wetting of glass surface using natural surfactants

2020 ◽  
Vol 12 ◽  
Author(s):  
Nihar Ranjan Biswal
Keyword(s):  
Contact Angle ◽  
Glass Surface ◽  
Pure Water ◽  
Amyl Alcohol ◽  
Synthetic Surfactant ◽  
Wide Range ◽  
Different Types ◽  
Natural Surfactants ◽  
Triton X ◽  
Solid Liquid

Background: Surfactant adsorption at the interfaces (solid–liquid, liquid–air, or liquid–liquid) is receiving considerable attention from a long time due to its wide range of practical applications. Objective: Specifically wettability of solid surface by liquids is mainly measured by contact angle and has many practical importances where solid–liquid systems are used. Adsorption of surfactants plays an important role in the wetting process. The wetting behaviours of three plant-based natural surfactants (Reetha, Shikakai, and Acacia) on the glass surface are compared with one widely used nonionic synthetic surfactant (Triton X-100) and reported in this study. Methods: The dynamic contact angle study of three different types of plant surfactants (Reetha, Shikakai and Acacia) and one synthetic surfactant (Triton X 100) on the glass surface has been carried out. The effect of two different types of alcohols such as Methanol and amyl alcohol on wettability of shikakai, as it shows little higher value of contact angle on glass surface has been measured. Results: The contact angle measurements show that there is an increase in contact angle from 47° (pure water) to 67.72°, 65.57°, 68.84°, and 68.79° for Reetha, Acacia, Shikakai, and Triton X-100 respectively with the increase in surfactant concentration and remain constant at CMC. The change in contact angle of Shikakai-Amyl alcohol mixtures are slightly different than that of methanol-Shikakai mixture, mostly there is a gradual increase in contact angle with the increasing in alcohol concentration. Conclusion: There is no linear relationship between cos θ and inverse of surface tension. There was a linear increase in surface free energy results with increase in concentration as more surfactant molecules were adsorbing at the interface enhancing an increase in contact angle.

scholarly journals Effects of urban atmospheric particulate matter on higher plants using Lycopersicon esculentum as model species

2021 ◽  
Vol 3 (9) ◽  
Author(s):  
Katalin Hubai ◽  
Nora Kováts ◽  
Gábor Teke
Keyword(s):  
Particulate Matter ◽  
Lycopersicon Esculentum ◽  
Protein Content ◽  
Higher Plants ◽  
Nutrient Content ◽  
Atmospheric Particulate Matter ◽  
Dose Effect ◽  
Atmospheric Particulate ◽  
Wide Range ◽  
Treatment Experiment

AbstractAtmospheric particulate matter (PM) is one of the major environmental concerns in Europe. A wide range of studies has proved the ecotoxic potential of atmospheric particles. PM exerts chemical stress on vegetation by its potentially toxic constituents; however, relatively few studies are available on assessing phytotoxic effects under laboratory conditions. In our study, aqueous extract of particulate matter was prepared and used for treatment. Experiment was following the procedure defined by the No. 227 OECD Guideline for the Testing of Chemicals: Terrestrial Plant Test. Tomato (Lycopersicon esculentum Mill.) plants were used; elucidated toxicity was assessed based on morphological and biochemical endpoints such as biomass, chlorophyll-a and chlorophyll-b, carotenoids, and protein content. Biomass reduction and protein content showed a clear dose–effect relationship; the biomass decreased in comparison with the control (100%) in all test groups (TG) at a steady rate (TG1: 87.73%; TG2: 71.77%; TG3: 67.01%; TG4: 63.63%). The tendency in protein concentrations compared to the control was TG1: 113.61%; TG2: 148.21% TG3: 160.52%; TG4: 157.31%. However, pigments showed a ‘Janus-faced’ effect: nutrient content of the sample caused slight increase at lower doses; actual toxicity became apparent only at higher doses (chlorophyll-a concentration decrease was 84.47% in TG4, chlorophyll-b was 77.17%, and finally, carotene showed 83.60% decrease in TG4).

scholarly journals Characterization of glycoconjugates of human gastrointestinal mucosa by lectins. II. Lectin binding to the isolated glycoproteins of normal and malignant gastric mucosa.

1984 ◽  
Vol 32 (7) ◽  
pp. 690-696 ◽  
Author(s):  
J Fischer ◽  
G Uhlenbruck ◽  
P J Klein ◽  
M Vierbuchen ◽  
R Fischer
Keyword(s):  
Molecular Weight ◽  
Gastric Mucosa ◽  
High Molecular Weight ◽  
Lectin Binding ◽  
Gel Filtration ◽  
Molar Ratio ◽  
Gastric Cancer Cells ◽  
Tissue Sections ◽  
Gradient Gel Electrophoresis ◽  
Triton X

Using affinity chromatography on HPA-, PNA-, Con A, and WGA-agarose columns only a part (10-30%) of the high molecular weight mucous glycoproteins could be isolated from the Triton X-100 solubilized components of normal as well as carcinomatous gastric mucosa. The main part of the mucus was not bound by the lectins, which corresponds to our earlier lectin histochemical observations on paraffin-embedded tissue sections. The lectin-bound mucous glycoproteins had a relatively lower molecular weight, ranging from about 250-1,000 kilodaltons, as indicated by polyacrylamide gradient gel electrophoresis and by gel filtration on Biogel A 1.5 m column. In gas chromatographic analysis the molar ratio of aminohexoses to galactose was found to be much higher (3:1) in the lectin-bound mucous substances than in the whole high molecular weight mucus (1:1). This finding indicates that lectins have a higher affinity to the hexosamine rich components of mucus, which may be special forms of mucous glycoprotein molecules or the incompletely glycosylated core and backbone regions of the oligosaccharide chains of mucus. Extremely high hexosamine values (10:1) were found in the PNA isolated mucus of gastric adenocarcinoma. Since it is known that PNA binds to the terminal disaccharide, beta-galactose-(1-3)-N-acetylgalactosamine, which is localized at the reducing end of the oligosaccharide chains of mucus, it is highly probable that the elongation of the oligosaccharide side chains is disturbed in gastric cancer cells.

A novel EP-involved pathway for iron release from soya bean seed ferritin

2010 ◽  
Vol 427 (2) ◽  
pp. 313-321 ◽  
Author(s):  
Xiaoping Fu ◽  
Jianjun Deng ◽  
Haixia Yang ◽  
Taro Masuda ◽  
Fumiyuki Goto ◽  
...  
Keyword(s):  
Serine Protease ◽  
Protein A ◽  
Soya Bean ◽  
Iron Release ◽  
Bean Seed ◽  
Specific Domain ◽  
Exterior Surface ◽  
Legume Seeds ◽  
Seedling Germination ◽  
Ph Range

Iron in phytoferritin from legume seeds is required for seedling germination and early growth. However, the mechanism by which phytoferritin regulates its iron complement to these physiological processes remains unknown. In the present study, protein degradation is found to occur in purified SSF (soya bean seed ferritin) (consisting of H-1 and H-2 subunits) during storage, consistent with previous results that such degradation also occurs during seedling germination. In contrast, no degradation is observed with animal ferritin under identical conditions, suggesting that SSF autodegradation might be due to the EP (extension peptide) on the exterior surface of the protein, a specific domain found only in phytoferritin. Indeed, EP-deleted SSF becomes stable, confirming the above hypothesis. Further support comes from a protease activity assay showing that EP-1 (corresponding to the EP of the H-1 subunit) exhibits significant serine protease-like activity, whereas the activity of EP-2 (corresponding to the EP of the H-2 subunit) is much weaker. Consistent with the observation above, rH-1 (recombinant H-1 ferritin) is prone to degradation, whereas its analogue, rH-2, becomes very stable under identical conditions. This demonstrates that SSF degradation mainly originates from the serine protease-like activity of EP-1. Associated with EP degradation is a considerable increase in the rate of iron release from SSF induced by ascorbate in the amyloplast (pH range, 5.8–6.1). Thus phytoferritin may have facilitated the evolution of the specific domain to control its iron complement in response to cell iron need in the seedling stage.

scholarly journals Determination of perfluorinated compounds (PFCs) in the fine particulate matter (PM2.5) in China

2016 ◽  
Vol 5 (1) ◽  
pp. 21-27
Author(s):  
Md Baki Billah
Keyword(s):  
Hong Kong ◽  
Particulate Matter ◽  
Octanoic Acid ◽  
Limit Of Detection ◽  
Fine Particulate Matter ◽  
Consumer Products ◽  
Nonanoic Acid ◽  
Dodecanoic Acid ◽  
Fine Particulate ◽  
Wide Range

Perfluorinated chemicals (PFCs) can be absorbed on fine particulate matter (PM2.5) and used as stain, water and grease repellent in a wide range of consumer products. Among the PFCs, perfluorooctane sulfonate (PFOS) and perfluoro octanoic acid (PFOA) are widely detected in human blood and serum and are of concern due to their potential toxicity. In the present experiment, fine particulate matter (PM2.5) from some northern (Beijing, Xian) and southern (Hong Kong, Guangzhou and Xiamen) cities of China were collected and analyzed for perfluoro butanoic acid (PFBA), perfluoro hexanoic acid (PFHxA), perfluoro octanoic acid (PFOA), perfluoro nonanoic acid (PFNA), perfluoro decanoic acid (PFDA), perfluoro undecanoic acid (PFUdA), perfluoro dodecanoic acid (PFDoA), perfluoro hexanesulfonate (PFHxS) and perfluoro octanesulfonate (PFOS) using liquid chromatography mass spectrometry (LC-MS/MS). The total PFCs ranged from 121.2 to 192.2pg/m3, leading by Guangzhou followed by Xian, Beijing, Xiamen and Hong Koung. Among the nine measured PFCs compounds, the level of PFHxS was below the limit of detection in all the sampling cities. The other eight PFCs (PFOS, PFDoA, PFUdA, PFDA, PFNA, PFOA, PFHxA and PFBA) were detected in all the sampling locations except PFDoA in Hong Kong samples. Human exposure estimated to PFCs for adults showed PFOS as the dominant inhaled compound representing 1.59, 1.15, 1.0 and 1.0 ng/day exposure for Hong Kong, Guangzhou, Xiamen, Beijing and Xian respectively. Results from this study contribute to our understanding of exposure pathways of PFCs to humans.Jahangirnagar University J. Biol. Sci. 5(1): 21-27, 2016 (June)

Urinary protein as measured with a pyrogallol red-molybdate complex, manually and in a Hitachi 726 automated analyzer.

1986 ◽  
Vol 32 (8) ◽  
pp. 1551-1554 ◽  
Author(s):  
N Watanabe ◽  
S Kamei ◽  
A Ohkubo ◽  
M Yamanaka ◽  
S Ohsawa ◽  
...  
Keyword(s):  
Trichloroacetic Acid ◽  
Protein Concentration ◽  
Urinary Protein ◽  
New Method ◽  
Normal Range ◽  
Pyrogallol Red ◽  
Wide Range ◽  
Biuret Method

Abstract In this new method for determining urinary protein, the reaction is complete within 10 min at 37 degrees C. This method is applicable to automated as well as manual measurements. Protein concentration and absorbance at 600 nm are linearly related throughout a wide range of concentrations, 10 to 16 000 mg/L. However, the chromogenicity of the gamma-globulins in this method is 70% of that of albumin, as estimated from results by a biuret method. Within-run CVs were less than 3.3%; the day-to-day CV was 2.9%. Errors due to interfering components in urine are less than 2%. The normal range for urinary protein as measured by this method was from 28 to 141 mg/day. Results by this method (y) and by a trichloroacetic acid-biuret method (x) correlated well (n = 80, r = 0.995; y = 0.99x - 2.9).

Dietary iodine and accumulation of radioiodine in thyroids of chickens.

1978 ◽  
Vol 234 (2) ◽  
pp. E168 ◽  
Author(s):  
W S Newcomer
Keyword(s):  
Growth Rates ◽  
Half Life ◽  
Trichloroacetic Acid ◽  
Turnover Rate ◽  
Iodine Content ◽  
White Leghorn ◽  
General Terms ◽  
Binding Rate ◽  
Wide Range ◽  
Fractional Turnover

Groups of hatchling white leghorn cockerels were fed a basic, semisynthetic low-iodine diet to which various amounts of iodide were added: 0 up to 100 microgram I/g feed. Growth rates were unaffected by the range of dietary iodine content. A transitory goiter occurred only in the group fed the basic low-I diet. Half-life of 131I in the thyroid increased directly and fractional turnover rate inversely with increase of dietary iodine. Trichloroacetic acid-soluble iodine in serum did not accurately reflect variation of dietary iodine. However, effective clearance and binding rate constants did decrease proportionally to dietary iodine increase beyond the first 3-5 days. Concentration of iodine in thyroid was directly proportional to dietary iodine after 10 days. There was no consistent correlation between thyroxine or triiodothyronine and dietary iodine except in general terms of high and low dietary iodine groups. The avian thyroid exhibits great lability in adjusting functionally to a wide range of dietary iodine contents.

Emission Distribution and Regulation of Local Heat Source

2012 ◽  
Vol 326-328 ◽  
pp. 330-334 ◽  
Author(s):  
Jan Valíček ◽  
Jana Müllerová ◽  
Vlastimil Kuběna ◽  
Pavel Koštial ◽  
Marta Harničárová ◽  
...  
Keyword(s):  
Particulate Matter ◽  
Chemical Composition ◽  
Human Health ◽  
Fossil Fuels ◽  
Local Heating ◽  
Point Of View ◽  
Total Production ◽  
Economic Point ◽  
Automated Method ◽  
Wide Range

Pollutants can be classified according to their chemical composition, harmfulness, hazardousness, risk rate and toxicity. The most monitored pollutants are particulate matter (PM), carbon monoxide (CO), nitrogen oxide (NOx), sulfur dioxide (SO2), organic substances which are in the form of gaseous phase in waste gases expressed as total organic carbon, dibenzodioxins and dibenzofurans [1-3]. Other pollutants are divided into several groups and subgroups, such as substances with carcinogenic effects (asbestos, Co, Cd, Be, Ni, As, Cr, dioxins, etc.), solid inorganic contaminants (He, Se, animony, and others) and inorganic pollutants in the form of gases (HCl, HF, ammonia, etc.), organic gases and vapours (phenol, toluene, acetone and many others) and gases causing the greenhouse effect (CO2, methane, N2O, hydrofluorocarbons, etc.). The term particulate matter, or suspended matter refers to the emissions of a wide range of wind drift solids and liquid particles of material in size from several nanometres up to 0.5 mm, which stay in the air for some time. This is a major component of atmospheric pollution, which contributes to harmful effects not only on human health but also on intensity of materials degradation. Into the atmosphere, where we can meet them, regardless of particle size and chemical composition, in the form of a complex heterogeneous mixture, they are released from burning fossil fuels and also from burning biomass-based fuels, while domestic heating accounts for about 16% of the total production of particulate matter [4,5,6]. This percentage represents a degree of imperfect combustion of fuels used in local heating. Emissions from incomplete combustion are undesirable from the point of view of human health as well as from the economic point of view, because this leads to the degradation of materials. Nevertheless, since fuel combustion is necessary for the society, emissions are still produced. This paper presents an automated method of perfect combustion control in local heating in order to minimize emissions being produced.

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