Using Daphnia magna Straus to monitor toxicity of sustainable oil sludge bio-phytoremediation

Oil sludge (OS) is the toxic product of various oil refinery processes. Oil sludge biophytoremediation is sustainable remediation efforts were conducted to reduce OS toxicity level and they also need to be monitored through toxicity test. In this study, the toxicants were OS postbiophytoremediation for 24 months (OS24) and 40 months (OS40), and were tested to Daphnia magna. The toxicity test consisted of Range Finding Test, acute and chronic toxicity test. The purpose of this research is to obtain the LC50 value of each OS and the chronic effects shown by D. magna. The method in this study was experimental with Completely Randomized Design (CRD). The LC50-96 hours values were calculated from 50% of dead individuals while D. magna reproduction was calculated from the number of neonates (offspring) produced within three weeks. The LC50 of OS24 and OS40 were 0.042 ppm and 0.366 ppm respectively. Both were categorized as ‘extremely toxic’ refer to Swan, 1994, observed chronic effects were reproduction delays and a decrease in the number of neonates.

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PENGARUH EKSTRAK DAUN KUNYIT (Curcuma longa Linn.) SEBAGAI INSEKTISIDA ELEKTRIK TERHADAP MORTALITAS NYAMUK Culex sp. L.

Pro-Life ◽

10.33541/pro-life.v6i1.937 ◽

2019 ◽

Vol 6 (1) ◽

pp. 44

Author(s):

Aseptianova Aseptianova

Keyword(s):

Disease Prevention ◽

Toxicity Test ◽

Curcuma Longa ◽

Research Result ◽

Probit Analysis ◽

Turmeric Extract ◽

Natural Insecticide ◽

Randomized Design ◽

Completely Randomized Design ◽

Culex Sp

Mosquito Culex sp. L. is a vector of elephantiasis disease, prevention effort of elephantiasis vector is usually done by using chemical and natural insecticide. In the leaves of turmeric can be used as a natural insecticide in the prevention of elephantiasis vector disease. The objectives of this research are (1) to know the effect of turmeric extract ((Curcuma longa Linn.) As electrical insecticide to mortality of Culex sp. L. (2) To know the concentration of turmeric extract (Curcuma longa Linn.) Which is effective to be used as an electric insecticide against mortality of Culex sp. L. expressed by the toxicity test of LC50 and LT50. The method used in this research is an experimental method with Completely Randomized Design (RAL) which consists of 5 treatments and 4 replications. Based on research result of turmeric extract (Curcuma longa Linn.) Have a very significant effect on mortality of Culex sp. L. with sig value 0,000 <sig value. 0.05. The probit analysis of LC50 of turmeric leaves extract on mortality of Culex sp. L. is 44, 840 ml, meaning that at that concentration has occurred 50% mortality of total mosquito. The probit analysis of LT50 turmeric leaves extract on mortality of Culex sp. L. for 1.825 hours. Keywords: turmeric leaves extract, mortality Culex sp. L. mosquito

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Nanoparticle of Silver Nitrate (Ag2NO3) and Carbamate (CO2NH3 derivatives) For Vector Control of Aedes aegypti

E3S Web of Conferences ◽

10.1051/e3sconf/201912516005 ◽

2019 ◽

Vol 125 ◽

pp. 16005

Author(s):

Mursid Raharjo ◽

Agus Subagyo ◽

Sulistyani

Keyword(s):

Aedes Aegypti ◽

Vector Control ◽

Toxicity Test ◽

Experimental Approach ◽

Infectious Agent ◽

Laboratory Research ◽

Nano Silver ◽

Randomized Design ◽

New Form ◽

Completely Randomized Design

The climate change has an impact on various lives, including in the vector breeding. The Aedes aegypti resistance as an infectious agent of dengue fever is harmful for life, especially to non-target organisms and the environment. Nano-silver (Ag2NO3), is a new form of engineering in vector control. The aim of this research was the toxicity test of nano-silver (Ag2NO3) and carbamate (CO2NH3 derivatives) on Aedes aegypti larvae. This was a laboratory research (true experiment), used for 3-4 instar stage larvae toxicity test . The number of samples was calculated using an experimental approach, with a completely randomized design. As simply, can formulated as follows, (t-1) (r-1)> 15. The amount of this samples were 14. The toxicity test with carbamate compounds, at concentration 0.01 mg / l as many as 80% larvae were death, whereas on concentrations 0.5 mg / l as many as 92% of the test animals were death. Toxicity test with nano-silver on concentration 0.3 mg / l, all of the test animals (100%) experienced were death. The results of the observations also showed that the instar more active in the low cocentration of Ag2NO3 (0.01 mg/l - 0.1 mg/l). The conclusion are, nano-silver is more effective than carbamate compounds, and there is a potential for resistance.

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Uji Toksisitas Bahan Aktif Niklosamida Terhadap Crustacea Sebagai Water Treatment dalam Budidaya Udang Vannamei (Litopenaus vannamei)

Jurnal Penelitian Pertanian Terapan ◽

10.25181/jppt.v15i3.129 ◽

2017 ◽

Vol 15 (3) ◽

Author(s):

Aan Fahrizki ◽

Herman Yulianto Yulianto ◽

Asep Saefulloh

Keyword(s):

Water Treatment ◽

Active Ingredient ◽

Toxicity Test ◽

Residual Effect ◽

Probit Analysis ◽

Residual Effects ◽

Toxicity Tests ◽

Randomized Design ◽

Completely Randomized Design ◽

Concentration Levels

This study aims to determine the level of toxicity of the active ingredient based niclosamide on the value LC50- 24h against crustaceans and the duration of the residual effect of niclosamide in water. Methods This study used a completely randomized design with 4 different concentration levels and 0 ppm as a control. Toxicity tests using a concentration of 1.7783; 3.1623; 5.6235 and 10.0002 ppm. To determine the duration of the test using the residual effects of detoxification. In the detoxification test stick with a concentration in the toxicity test. Probit analysis results in test animals showed values (LC50) - 24 hours amounted to 3.6282 ppm, while the length of the residual effects of niclosamide in water for 96 hours. Kata kunci : Crustacea, niclosamide, and toxicity

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Kandungan Nutrisi Lumpur Sawit Hasil Fermentasi dengan Jamur P. chrysosporium

Jurnal Agripet ◽

10.17969/agripet.v13i2.820 ◽

2013 ◽

Vol 13 (2) ◽

pp. 47-52 ◽

Cited By ~ 1

Author(s):

Noferdiman Noferdiman ◽

Ahmad Yani

Keyword(s):

Experimental Design ◽

Palm Oil ◽

Combination Treatment ◽

Significant Interaction ◽

Crude Protein ◽

The Other ◽

Oil Sludge ◽

Crude Fiber ◽

Randomized Design ◽

Completely Randomized Design

The nutritious content fermentation of palm oil sludge by P. chrysosporiumABSTRACT. This research is aimed at observing the nutritious content of palm oil sludge fermentation by P. chrysosporium. The experimental design used completely randomized design in factorial (3 x 3). The first factor is the inoculum dosage, namely : (D1). 3 %, (D2). 6 % and (D3). 9 % of substrate weight. The second factor is the length of fermentation, namely : (L1). 4 days, (L2). 8 days and (L3). 12 days. Every treatment is repeated for three time. The data found is scrutinized by mode print and it is followed by distance test of multiple Duncan (Steel and Torrie, 1989). The variables observed in research are dry materials, crude fiber, crude protein, cellulose and lignin. The research on the influence of inoculum dosage and the length of fermentation on dry materials content and crude protein shows insignificant interaction (P0.05). On the other hand, crude fiber, cellulose and lignin show significant interaction (P0.01). The inoculum dosage of 6 % and the fermentation length of 8 days is the best combination treatment and it can reduce the crude fiber amounting to 40.86 %, an increase of crude protein amounting to 30.75 % and a decrease of cellulose and lignin amounting to 39.78 % and 36.40 % respectively.

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KECERNAAN RANSUM SAPI BALI DENGAN KONSENTRAT FERMENTASI BERBASIS LUMPUR SAWIT DAN BAHAN PAKAN LOKAL

AGROINTEK ◽

10.21107/agrointek.v11i2.3173 ◽

2017 ◽

Vol 11 (2) ◽

pp. 63

Author(s):

Irma Badarina ◽

Jarmuji Jarmuji ◽

Dodi Permadi Gultom

Keyword(s):

Organic Matter ◽

Palm Oil ◽

Dry Matter ◽

Live Weight ◽

Oil Sludge ◽

Coffee Husk ◽

Concentrate Supplementation ◽

Randomized Design ◽

Completely Randomized Design

The aim of this research was to evaluate the dry matter and organic matter<br />digestibility in vivo of Bali cattle diet that supplemented with fermented concentrate ration based from palm oil sludge and several local feed sources. Fifteen Bali cattles with 2 years old and the range of live weight 184,5±3,63 kg were distributed into three treatments and five replication in Completely Randomized design. The treatment was the level of concentrate supplementation, They were P1 (10kg/day), P2(7,5kg/day) and P3(5kg/day). The forage and the rice straw were adjusted as much as 10 kg/day and 1 kg/day, respectively. The composition of fermented concentrate consisted of palm oil sludge (70%), rice bran (10%), coffee husk (10% ) and coconut press fiber (10%). As bioactivator used”Bionak”as much as 0,3%. The an aerob fermentation took time a week. The result showed that up to the highest level (10kg/day) the supplementation of fermented concentrate ration enhanced the consumption and the digestibility of the whole rations.

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Uji Mortalitas Ulat Grayak (Spodoptera litura F.) Terhadap Ekstrak Tanaman Lidah Mertua (Sansevieria trifasciata Prain)

Jurnal Bioterdidik: Wahana Ekspresi Ilmiah ◽

10.23960/jbt.v8i3.21591 ◽

2020 ◽

Vol 8 (3) ◽

pp. 56-67

Author(s):

Gusthi Permatasari ◽

◽

Nova Hariani ◽

Sus Trimurti ◽

◽

...

Keyword(s):

Life Cycle ◽

Plant Extract ◽

Research Method ◽

Spodoptera Litura ◽

Ethanol Extract ◽

Agricultural Pests ◽

Lc50 Value ◽

Rotary Evaporator ◽

Randomized Design ◽

Completely Randomized Design

The tongue-in-law plant (Sansevieria trifasciata Prain) contains saponins, flavonoids, steroids and triterpenoids which are natural metabolites that have the potential to control agricultural pests such as armyworms (Spodoptera sp.). Therefore, the aim of this study was to determine the mortality and LC50 value for 72 hours of giving in-law tongue plant extract to Spodoptera litura and the length of the life cycle of S. litura in the laboratory. The research method used a completely randomized design (CRD). The extract of the tongue-in-law plant is obtained from the maceration process of the tongue-in-law plant with ethanol and then concentrated using a rotary evaporator until the thick extract is obtained. The results of the research on the ethanol extract of the tongue-in-law plant on the mortality of S. litura, found that the highest mortality was 63.6% at a concentration of 800 ppm and the lowest mortality value was 36.4% at a concentration of 200 ppm for 72 hours.

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Sublethal toxicity test of mercury (Hg) in the flesh and tissue of Tilapia (Oreochromis niloticus)

Omni-Akuatika ◽

10.20884/1.oa.2020.16.2.749 ◽

2020 ◽

Vol 16 (2) ◽

pp. 99

Author(s):

Yusnaini Yusnaini ◽

Emiyarti Emiyarti ◽

Indriyani Nur ◽

Oce Astuti ◽

Rahmad Sofyan Patadjai

Keyword(s):

Tissue Damage ◽

Oreochromis Niloticus ◽

Toxicity Test ◽

Control Group ◽

Sublethal Toxicity ◽

Mononuclear Cell Infiltration ◽

Tubular Necrosis ◽

Randomized Design ◽

Completely Randomized Design ◽

Hg Accumulation

This study tested the sublethal toxicity of mercury (Hg) in the flesh and tissue of tilapia (Oreochromis niloticus). This research used an experimental method completely randomized design (CRD) with 3 treatments and 3 replications. The treatments used different Hg concentrations: Treatment A = 0.08 ppm; B = 0.16 ppm; C = 0.24 ppm; and the control group = without the addition of Hg. The parameters observed were the accumulation of mercury (Hg) in the flesh and tissue damage (histopathology) of the gills, kidneys and liver of tilapia. The results showed that the highest Hg accumulation was at the highest Hg concentration (treatment C = 0.24 ppm). Tissue damage was mainly found in the kidneys, liver and gills of fish exposed to Hg with indications of bleeding, tubular necrosis, vacuolization of epithelial cells, and mononuclear cell infiltration. The results showed that the toxicity of mercury to the organs increased with the increase in the concentration of Hg in water. Keywords : flesh, mercury, Oreochromis niloticus, sublethal, tissue

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Pengaruh Suhu Dan Kepadatan Ephippia Yang Berbeda Terhadap Penetasan Ephippia Daphnia magna

Jurnal Ilmiah Perikanan dan Kelautan ◽

10.20473/jipk.v1i1.11695 ◽

2019 ◽

Vol 1 (1) ◽

pp. 31

Author(s):

Yulian Cindra Eka Pradana, Boedi Setya Rahardja, Yudi Cahyoko

Keyword(s):

Water Quality ◽

Daphnia Magna ◽

Food Source ◽

The Other ◽

High Population Density ◽

Experimental Characteristic ◽

Randomized Design ◽

Good Nutrition ◽

Completely Randomized Design ◽

Environment Condition

Abstract Daphnia magna has good nutrition to carry on egg produce and fry of fish cupang, maskoki, oscar, tetra, and also can used for food source of fry and seed. Daphnia magna not only supply from natural environment, cause their growth deppend on fertilizer as food and environment condition. The other side: high population density, limited of food source low temperature and shorter irradiation can produce dorman egg (ephippia). The dorman eeg can use for Daphnia magna stock which way by hatching. The factor that can be influence for ephippia hatch are temperature and density. Based on this fact, a research about influence of different temperature and ephippia density for ephippia hatch of Daphnia magna. The purpose of this research was to know the influence of different temperature and ephippia density for ephippia hatch of Daphnia magna. This research has experimental characteristic and use Factorial Completely Randomized Design with three experiments of temperature (A factor), three experiments of density (B factor) and three times replicated. There are 9 kinds of combination between density and temperature. The parameter which checked in this research is degree of hatch of ephippia Daphnia magna and water quality that is dissolve oxygen rate, NH3 and of pH hatch media. The result of the analysis showed that the temperature and density influence of hatching Daphnia magna ephippia. The best result can be used for hatching Daphnia magna ephippia is temperature at 25oC with density 50 ephippia /100 ml giving highest result for the hatch of ephippia Daphnia magna. There are interaction betwen temperature and density for hatching.

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Efek sitotoksik ekstrak buah kapulaga jawa (Amomum compactum Soland. Ex. Maton)

Jurnal SAGO Gizi dan Kesehatan ◽

10.30867/gikes.v1i1.302 ◽

2019 ◽

Vol 1 (1) ◽

pp. 79

Author(s):

Nailul Muna ◽

Noni Zakiah ◽

Vonna Aulianshah ◽

Munira Munira ◽

Amelia Sari

Keyword(s):

Cell Damage ◽

Developed Countries ◽

Artemia Salina ◽

Oxidation Reactions ◽

Treatment Groups ◽

Lc50 Value ◽

Randomized Design ◽

Completely Randomized Design ◽

Amomum Compactum ◽

Anticancer Compound

Background: In developed countries cancer is the second leading cause of death after cardiovascular disease. Cancer prevention can be done with synthetic or traditional medicine. However, synthetic treatments often cause side effects and of course require high costs. Java Cardamom (Amomum compactum Soland. Ex Maton) is a plant that contains antioxidants. Antioxidants can inhibit oxidation reactions by binding to free radicals and molecules that are very reactive so that cell damage can be prevented. Cardamom contains flavonoids which can induce cell death programs as well as tannins as free radical scavengers.Objectives: This study aims to determine the LC50 value of Java cardamom (Amomum compactum Soland. Ex Maton) on Artemia salina Leach.Methods: This study was an experimental study using a completely randomized design (CRD) using 180 artemia larvae which were divided into 6 treatment groups. Each group included 10 artemia larvae with varying concentrations of 500 ppm, 250 ppm, 100 ppm, 50 ppm, 10 ppm and 0 ppm (not given extract). Three repetitions were performed for each concentration.Results: The results showed from the observations obtained the LC50 value of Java cardamom extract is 26.60 ppm. So that the Java cardamom extract (Amomum compactum Soland. Ex Maton) has the potential as an anticancer (cytotoxic).Conclusion: Java cardamom extract (Amomum compactum Soland. Ex Maton) is active and can be developed as an anticancer compound.

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Uji Toksisitas Letal dan Subletal Logam Berat Merkuri (Hg) Terhadap Ikan Nilem (Osteochilus hasselti)

Omni-Akuatika ◽

10.20884/1.oa.2016.12.1.68 ◽

2016 ◽

Vol 12 (1) ◽

Cited By ~ 1

Author(s):

Norman Arie Prayogo ◽

Atik Hidayati ◽

Asrul Sahri Siregar

Keyword(s):

Heavy Metal ◽

Toxicity Test ◽

Aquatic Organisms ◽

Sublethal Effect ◽

Aquatic Organism ◽

Toxicity Tests ◽

Sublethal Toxicity ◽

Lethal Toxicity ◽

Randomized Design ◽

Completely Randomized Design

Mercury (Hg) is a heavy metal could pollute the river. Hg impacts on aquatic organism chronically. The influence of chemical toxic on aquatic organisms could be determined using toxicity (lethal and sublethal) tests. Osteochilus hasselti could be an object for toxicity test. A research, aiming to find LC50-96h concentration, was to find letal and subletal effects of Hg on erythrocyte and hematocryte changes. An experimental method applied Completely Randomized Design. The research was devided into 3 stages, i.e. preliminary, lethal toxicity (LC50-96h) and sublethal toxicity tests, in triplicates. Sampling of sublethal test was performed after an exposure time of 96 h (4 days) and 288 h (12 days). Lethal toxicity test data were analyzed as probit and data from sublethal toxicity test were F-tested. The result showed that LC50-96h of Hg on Osteochilus hasselti was 0.39 mg/L. The sublethal effect of Hg decreased erythrocyte ad hematocryte counts in parallel with increasing its concentration and its exposure time.Keywords: Hg, toxicity, LC50-96 hours, erythrocytes, hematocrits.

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