scholarly journals Antibacterial and antioxidant activities of ethanol extract of sukun (Artocarpus altilis.) leaves against Pseudomonas aeruginosa

2021 ◽  
Vol 332 ◽  
pp. 08006
Author(s):  
Effendy De Lux Putra ◽  
Henni Cintya ◽  
Denny Satria
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Ethanol Extract ◽  
The Body ◽  
Artocarpus Altilis ◽  
Concentration Method ◽  
Calcium Phosphorus ◽  
Herbal Plant ◽  
Cosmetic Medicine

Antioxidants are thought to help the body fight Reactive Oxygen Species (ROS). Antioxidants are defined as “any substance that significantly slows or inhibits oxidation when present in trace amounts relative to an oxidizable substrate.” Antimicrobials generated from plants have been used to treat infectious diseases for millennia. Many investigations have been done on the antibacterial effects of herbal plant extracts., including roots, stems, leaves, and flowers. Artocarpus altilis is a versatile tree plant that is a rich source of calcium, phosphorus, carbohydrates, minerals, and vitamins. Various parts of breadfruit are used as food, cosmetic medicine, clothing, treating diarrhea, high blood pressure, and asthma. This study obtained about determine biological activities of Artocarpus altilis Leaves. The extract was prepared using water with the soxhletation method. The antibacterial activity was determined with minimun inhibitor concentration method with concentration 300-25 mg/mL can inhibit the growth of Pseudomonas aeruginosa, The MIC is the lowest concentration that may stop bacterial growth, and the number of bacteria is 10 colonies. 60.13 0.54 ppm antioxidant activity, fenol (224.67 mg GAE/g) and flavonoids (27.63 mg QE/g). The results show that Artocarpus altilis leaf ethanol extract is antibacterial and antioxidant.

Determination of Antioxidant Activities and Bioactive Components of Foeniculum vulgare Mill. (Samonsabar)

2019 ◽  
Vol 30 (3) ◽  
pp. 16-22
Keyword(s):  
Antioxidant Activities ◽  
Biological Activities ◽  
Scientific Information ◽  
Ethanolic Extract ◽  
The Body ◽  
World Health ◽  
Gas Chromatography Mass Spectrometry ◽  
Molecular Formula ◽  
Bioactive Components ◽  
Foeniculum Vulgare

World Health Organization (WHO) estimated that 80% of the population of developing countries use traditional medicines, mostly natural plant products, for their primary health care needs. In the past few decades, the medicinal value of plants has been assumed more important dimension owing largely to the discovery that extracts from plants contain not only primary metabolites but also a diverse array of secondary metabolites with antioxidant potential. Medicinal plants are potential sources of natural compounds with biological activities and therefore attract the attention of researchers worldwide. Antioxidants are vital substances which possess ability to protect the body from damage due to free radical-induced oxidative stress. The purpose of current study was to determine the antioxidant activities and bioactive components of Foeniculum vulgare (fennel) (Samonsabar) seeds by using UV Visible Spectrophotometer (UV-Vis) and Gas Chromatography-Mass Spectrometry (GC-MS). Aqueous extract of fennel seeds showed more antioxidant activity (IC50: 0.28 ug/ml) than ethanolic extract (IC50: 0.83 ug/ml) and comparable to standard antioxidant, ascorbic acid (IC50: 0.59 ug/ml). GC-MS analysis was fruitful in identification of compounds based on peak area, retention time, molecular formula, molecular weight, MS Fragmentions and pharmacological actions. Ten bioactive phytochemical compounds from aqueous extracts and 11 from ethanolic extract of fennel seeds were identified. These findings indicated that fennel seeds are potential to provide preventive properties against oxidative damage. These results will give scientific information for quality control of indigenous drug to herbal medicine users and local practitioners using fennel for different types of ailments

scholarly journals The Chemical Composition, Antioxidant and Anti-Tyrosinase Activities, and Irritation Properties of Sripanya Punica granatum Peel Extract

Cosmetics ◽  
2020 ◽  
Vol 7 (1) ◽  
pp. 7 ◽  
Author(s):  
Nachtharinee Laosirisathian ◽  
Chalermpong Saenjum ◽  
Jakkapan Sirithunyalug ◽  
Sukum Eitssayeam ◽  
Busaban Sirithunyalug ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Chorioallantoic Membrane ◽  
Ethanol Extract ◽  
Punica Granatum ◽  
Total Phenolic ◽  
Peel Extract

This is the first study to investigate the effect of ethanol concentrations on the phenolic content and biological activities of Sripanya Punica granatum peel extract. Various concentrations of ethanol, (20 to 80% v/v), were used in the maceration process. Extracts were investigated for total phenolic content by the Folin–Ciocalteu method, antioxidant activities by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay, and anti-tyrosinase activity by a spectrophotometric method. The chemical composition was investigated using high-performance liquid chromatography. The irritation of the extracts was determined by Hen’s egg test–chorioallantoic membrane assay. The results demonstrated that E60, which was extracted using 60% v/v ethanol, had the highest yield (66.23 ± 0.38% w/w), the highest total phenolic content (490.42 ± 7.20 μg GAE/g), and the highest antioxidant and anti-tyrosinase activities. IC50 values on DPPH• and ABTS•+ inhibition were 10.97 ± 0.97 and 6.48 ± 0.06 µg/mL, respectively. The 60% v/v ethanol extract (E60) possessed strong tyrosinase inhibition with an IC50 value of 0.10 ± 0.13 µg/mL, which is more potent than kojic acid. The major constituents were punicalagin (4.05 ± 0.26% w/w) and ellagic acid (0.63 ± 0.04% w/w). E60 was safe for topical application since it did not induce irritation. Therefore, we suggest that E60 is a promising agent for further product development.

scholarly journals ANTIOXIDANT ACTIVITY OF SYZYGIUM AROMATIUM AND CINNAMOMUM VERUM SEED EXTRACTS

2021 ◽  
Vol 2021 (1) ◽  
Author(s):  
H Mahmood ◽  
Q Ali ◽  
MM Hafeez ◽  
A Malik
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Molecular Biology ◽  
Antioxidant Activities ◽  
Research Work ◽  
Ethanol Extract ◽  
Plant Seeds ◽  
Ethanol Extracts ◽  
Herbal Plant ◽  
Seed Extracts

The present study was carried out for the evaluation of antioxidant activities of clover (Syzygium aromatium L.) and cinnamon (Cinnamomum verum L.). The research work was carried out at the Institute of Molecular Biology, University of Lahore. The samples of clovers and cinnamon were collected from Lahore and were crushed to prepared sample in n-hexan, and ethanol extracts for evaluation of antioxidant activities. The highest antioxidant activities of ethanol extract from cinnamon indicated that the cinnamon may be used as an active ingredient to control stress conditions of cells for stress avoidance. The alkaloids, coumarins, saponins, flavonoids, tannins, sterols, quinons, triterpenoids, anthocyans, leucoanthocyans and terpenoids were tested for both ethanol and n-hexan extracts which indicated that most of the components were found present in both of the plant species, which revealed that these herbal plant seeds may be used as potential medicinal plants.

scholarly journals Antibacterial and antioxidant activities of ethanol extract of Artocarpus lacucha Buch-Ham. leaves against Propionibacterium acne

2021 ◽  
Vol 332 ◽  
pp. 08007
Author(s):  
Panal Sitorus ◽  
Mahatir Muhammad ◽  
Henni Cintya ◽  
and Denni Satria
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
The Body ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Degenerative Diseases ◽  
Inhibitor Concentration ◽  
Propionibacterium Acne

Antioxidants can neutralize free radicals that can affect biological systems in the body that can cause degenerative diseases. Antibacterial active molecules have been identified from plants, and these chemicals are being utilized to develop new and promising treatments. The Mobe plant (Artocarpus lacucha Buch-Ham.) is a species of Artocarpus member of the Moraceae family. Includes a high proportion of phenolic compounds (flavonoids and phenolic acid). This research obtained to know about the biological activity of Artocarpus lacucha Buch-Ham and their total flavonoid and total phenolic content. The extract was prepared using the soxhletation technique using ethanol as the solvent. Antibacterial activity was determined using minimum inhibitor concentration. Propionibacterium acne can be inhibited at concentrations ranging from 300 ppm to 25 ppm. The DPPH technique was used to assess antioxidant activity. The samples’ total flavonoid and phenolic content were measured colorimetrically. The DPPH test revealed an antioxidant activity of 23.33 g/mL, which was used to compute the IC50. The extract’s total phenolic (117.44 mg GAE/g) and flavonoid (3.54 mg QE/g) content were determined to be high.

scholarly journals Phytochemical screening and biological activities of pakoba (Syzygium luzonense) stem bark ethanol extract

2020 ◽  
Vol 21 (6) ◽  
Author(s):  
Mario Walean ◽  
ROSTINA MELPIN ◽  
MERVINA RONDONUWU ◽  
KINZIE FELICIANO PINONTOAN ◽  
Hendra Pratama Maliangkay ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Activity Index ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Optimum Dose ◽  
Phytochemical Analysis ◽  
Phytochemical Screening

Abstract. Walean M, Melpin R, Rondonuwu M, Pinontoan KF, Maliangkay HP, Astriani M. 2020. Phytochemical screening and biological activities of pakoba (Syzygium luzonense) stem bark ethanol extract. Biodiversitas 21: 2377-2382. Pakoba (Syzygium luzonense (Merr.) Merr) as an endemic and ethnomedical plant of North Sulawesi was investigated for its phytochemical constituents and biological activities. The presence of phytochemicals constituents, antibacterial, antioxidant activities, and antihyperglycemic of pakoba stem bark ethanolic extract (PSBEE) were also investigated. Pakoba stem bark was extracted using ethanol. The extracts were evaluated for antimicrobial activity using the Kirby Bauer method, whereas antioxidant activity was determined by using 1,1-diphenyl-2-picrylhydrazyl radical scavenging assay. Alloxan-induced diabetic rats investigation was used to determine the antihyperglycemic activity. The phytochemical analysis revealed that tannins, alkaloids, and flavonoids were present in PSBEE. PSBEE has potent antioxidant activity with antioxidant activity index = 1.02. PSBEE significantly reduced the blood sugar of hyperglycemic rats with an optimum dose of 300 mg/kgbw. In this study, PSBEE has weak antibacterial activity against in Escherichia coli and Staphylococcus aureus.

Comparison of drying & extraction methods on activity in ficus

2020 ◽  
Vol 9 (2) ◽  
pp. 18-21
Author(s):  
Avinash Kumar Reddy G ◽  
Ramani S L ◽  
Pallavi G ◽  
Surekha K ◽  
Vemaiah C H
Keyword(s):  
Side Effects ◽  
Physiological Stress ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Tectona Grandis ◽  
Extraction Methods ◽  
The Body ◽  
Methanol Extracts ◽  
Synthetic Drugs ◽  
Antioxidant Profile

Free radicals in the body are responsible for physiological stress and deterioration of the body functions. The antioxidants are used to fight the oxygen-free radicles and neutralize them. Apart from the potency of the drugs as antioxidants, there had been many reported side effects of synthetic drugs. Herbs are used as alternatives for the treatment of many diseases which are devoid of side effects. The current research focusses on the comparison of the antioxidant profile of the ethanol and methanol extracts of leaves of Tectona. Tectona grandis extracts were tested for antioxidant activities and compared with ethanol and methanol extracts in various methods. The results showed that the methanol extract showed better activity than that of the ethanol extract and when compared to the standard extracts showed higher antioxidant activity.

scholarly journals ANTIOXIDANT ACTIVITY OF ALKALOID FRACTIONS OF ZANTHOXYLUM ACANTHOPODIUM DC. FRUITS WITH 1,1-DIPHENYL-2-PICRYLHYDRAZYL ASSAY

2018 ◽  
Vol 11 (13) ◽  
pp. 33
Author(s):  
Rosidah Rosidah ◽  
Poppy Anjelisa Zaitun Hasibuan ◽  
Ginda Haro ◽  
Puteri Masri ◽  
Denny Satria
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Liquid Extraction ◽  
The Body ◽  
Antioxidant Potential ◽  
Liquid Liquid Extraction

 Objective: Antioxidants are vital substances which possess the ability to protect the body from damage caused by free radical-induced oxidative stress. The purpose of this study is to determine antioxidant activities of alkaloid fractions of Zanthoxylum acanthopodium DC. fruits.Methods: Z. acanthopodium DC. fruit was extracted by maceration. Ethanol extract was fractionated with liquid-liquid extraction using n-hexane and chloroform at pH 3, 7, 9, and 11 to obtained alkaloid fractions. Antioxidant activity for extract and fractions was determined with 1,1-diphenyl-2- picrylhydrazyl.Results: The IC50 of extract and fractions was 101.11±0.11, 273.24±0.12, 116.62±0.04, 61.12±0.01, 23.15±0.01, and 112.39±0.04 μg/mL, respectively.Conclusion: The results reveal that alkaloid fractions of Z. acanthopodium DC. fruit have very strong antioxidant potential. Our further study is to isolate the alkaloid compounds.

scholarly journals Synthesis, Characterization and Antibacterial Evaluation of Some Coumarin Derivatives

2021 ◽  
Vol 30 (1) ◽  
pp. 249-257
Author(s):  
Tariq T. Abduljabbar ◽  
Mohammed K. Hadi
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Diffusion Method ◽  
Coumarin Derivatives ◽  
Gram Positive Bacteria ◽  
Highly Active ◽  
Ft Ir ◽  
Anti Hiv ◽  
Ft Ir Spectroscopy

Coumarin derivatives have shown different biological activities, such as antifungal, antibacterial   antiinflammatory, and antioxidant activities, besides antibiotic resistance modulating effects, and anti-HIV, hepatoprotective, and antitumor effect. So, new coumarin derivatives (hydrazones and an amide) were synthesized through multisteps reactions. All the synthesized target compounds were characterized by FT-IR spectroscopy, 1HNMR analysis. The compounds then evaluated for their anti-bacterial activity by means of well-diffusion method against two gram-positive bacteria (Staphylococcus aureus, Streptococcus pneumoniae) and two gram-negative bacteria  (E.coli and Pseudomonas aeruginosa). The highest activity was demonstrate by compound k2 which found to be highly active against pseudomonas aeruginosa.

Improvement of cytoprotective and antioxidant activity of Rosa canina L. and Salix alba L. by controlled differential sieving process against H2O2-induced oxidative stress in mouse primary splenocytes

2017 ◽  
Vol 87 (3-4) ◽  
pp. 191-200 ◽  
Author(s):  
Nidhal Soualeh ◽  
Aliçia Stiévenard ◽  
Elie Baudelaire ◽  
Rachid Soulimani ◽  
Jaouad Bouayed
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Good Alternative ◽  
Activity Levels ◽  
Salix Alba ◽  
Rosa Canina ◽  
Free Process ◽  
Plant Powders ◽  
Sieving Process

Abstract. In this study, cytoprotective and antioxidant activities of Rosa canina (RC) and Salix alba (SA), medicinal plants, were studied on mouse primary splenocytes by comparing Controlled Differential Sieving process (CDSp), which is a novel green solvent-free process, versus a conventional technique, employing hydroethanolic extraction (HEE). Thus, preventive antioxidant activity of three plant powders of homogeneous particle sizes, 50–100 µm, 100–180 µm and 180–315 µm, dissolved directly in the cellular buffer, were compared to those of hydroethanolic (HE) extract, at 2 concentrations (250 and 500 µg/mL) in H2O2-treated spleen cells. Overall, compared to HE extract, the superfine powders, i. e., fractions < 180 µm, at the lowest concentration, resulted in greater reactive oxygen species (ROS) elimination, increased glutathione peroxidase (GPx) activity and lower malondialdehyde (MDA) production. Better antioxidant and preventive effects in pre-treated cells were found with the superfine powders for SA (i. e., 50–100 µm and 100–180 µm, both p < 0.001), and with the intermediate powder for RC (i. e., 100–180 µm, p < 0.05) versus HE extract. The activity levels of catalase (CAT) and superoxide dismutase (SOD) in pretreated splenocytes exposed to H2O2, albeit reduced, were near to those in unexposed cells, suggesting that pretreatment with the fine powders has relatively restored the normal levels of antioxidant-related enzymes. These findings supported that CDSp improved the biological activities of plants, avoiding the use of organic solvents and thus it could be a good alternative to conventional extraction techniques.

Phytochemical composition and antioxidant activity of Malus baccata (L.) Borkh. fruits

2020 ◽  
Vol 3 (1) ◽  
pp. 47
Author(s):  
Nadezhda Petkova ◽  
Manol Ognyanov ◽  
Blaga Inyutin ◽  
Petar Zhelev ◽  
Panteley Denev
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Titratable Acidity ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Frap Assay ◽  
Phytochemical Composition ◽  
Malus Baccata

Crab apple (Malus baccata (L.) Borkh.) was mainly distributed in Europe as an ornamental plant, but the nutritional properties of its edible fruits were not fully revealed. The aim of the current study was to characterize the phytochemical composition of ripen carb apple fruits and to evaluate their nutritional and antioxidant potentials. The fruits were assayed for moisture and ash content, proteins, lipids, carbohydrates, titratable acidity (TA), pH, total phenolic compounds and natural pigments. Among the analyzed carbohydrates cellulose was found in the highest content (6% dw), followed by sugars (sucrose, glucose and fructose) and 1.8 % dw uronic acids. The total chlorophylls and carotenoids contents in their fruits were 6.51 and 4.80 μg/g fw, respectively. Total monomeric anthocyanins were not detected. The highest content of total phenolic compounds (2.67 mg GAE/g fw) was found in 95 % ethanol extract from fruits, while the total flavonoids were relatively low – 0.1 mg QE/g fw. DPPH assay (17.27 mM TE/g fw) and FRAP assay (14.34 mM TE/g fw) demonstrated in vitro antioxidant activities of crabapple. Malus baccata fruits were evaluated as a rich source of dietary fibers and phenolic compounds with significant antioxidant potential that could be used in human nutrition.

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