scholarly journals Cell migration in complex environments: chemotaxis and topographical obstacles

2020 ◽  
Vol 67 ◽  
pp. 191-209 ◽  
Author(s):  
Alessandro Cucchi ◽  
Christèle Etchegaray ◽  
Nicolas Meunier ◽  
Laurent Navoret ◽  
Lamis Sabbagh
Keyword(s):  
Cell Migration ◽  
Threshold Value ◽  
External Signal ◽  
Complex Environments ◽  
Cell Dynamics ◽  
Contact Algorithm ◽  
A Cell ◽  
And Migration ◽  
The Impact ◽  
Force Intensity

Cell migration is a complex phenomenon that plays an important role in many biological processes. Our aim here is to build and study models of reduced complexity to describe some aspects of cell motility in tissues. Precisely, we study the impact of some biochemical and mechanical cues on the cell dynamics in a 2D framework. For that purpose, we model the cell as an active particle with a velocity solution to a particular Stochastic Differential Equation that describes the intracellular dynamics as well as the presence of some biochemical cues. In the 1D case, an asymptotic analysis puts to light a transition between migration dominated by the cell’s internal activity and migration dominated by an external signal. In a second step, we use the contact algorithm introduced in [15,18] to describe the cell dynamics in an environment with obstacles. In the 2D case, we study how a cell submitted to a constant directional force that mimics the action of chemoattractant, behaves in the presence of obstacles. We numerically observe the existence of a velocity value that the cell can not exceed even if the directional force intensity increases. We find that this threshold value depends on the number of obstacles. Our result confirms a result that was already observed in a discrete framework in [3,4].

scholarly journals Guiding cell adhesion and motility by modulating cross-linking and topographic properties of microgel arrays

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0257495
Author(s):  
Janine Riegert ◽  
Alexander Töpel ◽  
Jana Schieren ◽  
Renee Coryn ◽  
Stella Dibenedetto ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Migration ◽  
Cell Adhesion ◽  
Focal Adhesion ◽  
Sertoli Cells ◽  
Cellular Systems ◽  
Cross Linking ◽  
Cell Adhesion And Migration ◽  
And Migration ◽  
The Impact

Biomaterial-driven modulation of cell adhesion and migration is a challenging aspect of tissue engineering. Here, we investigated the impact of surface-bound microgel arrays with variable geometry and adjustable cross-linking properties on cell adhesion and migration. We show that cell migration is inversely correlated with microgel array spacing, whereas directionality increases as array spacing increases. Focal adhesion dynamics is also modulated by microgel topography resulting in less dynamic focal adhesions on surface-bound microgels. Microgels also modulate the motility and adhesion of Sertoli cells used as a model for cell migration and adhesion. Both focal adhesion dynamics and speed are reduced on microgels. Interestingly, Gas2L1, a component of the cytoskeleton that mediates the interaction between microtubules and microfilaments, is dispensable for the regulation of cell adhesion and migration on microgels. Finally, increasing microgel cross-linking causes a clear reduction of focal adhesion turnover in Sertoli cells. These findings not only show that spacing and rigidity of surface-grafted microgels arrays can be effectively used to modulate cell adhesion and motility of diverse cellular systems, but they also form the basis for future developments in the fields of medicine and tissue engineering.

scholarly journals Random Walks of a Cell With Correlated Speed and Persistence Influenced by the Extracellular Topography

2021 ◽  
Vol 9 ◽  
Author(s):  
Kejie Chen ◽  
Kai-Rong Qin
Keyword(s):  
Cell Migration ◽  
Random Walk ◽  
Cell Types ◽  
Coarse Grained ◽  
Search Efficiency ◽  
Complex Environments ◽  
Unit Distance ◽  
Lévy Walk ◽  
Levy Walk ◽  
A Cell

Cell migration through extracellular matrices is critical to many physiological processes, such as tissue development, immunological response and cancer metastasis. Previous models including persistent random walk (PRW) and Lévy walk only explain the migratory dynamics of some cell types in a homogeneous environment. Recently, it was discovered that the intracellular actin flow can robustly ensure a universal coupling between cell migratory speed and persistence for a variety of cell types migrating in the in vitro assays and live tissues. However, effects of the correlation between speed and persistence on the macroscopic cell migration dynamics and patterns in complex environments are largely unknown. In this study, we developed a Monte Carlo random walk simulation to investigate the motility, the search ability and the search efficiency of a cell moving in both homogeneous and porous environments. The cell is simplified as a dimensionless particle, moving according to PRW, Lévy walk, random walk with linear speed-persistence correlation (linear RWSP) and random walk with nonlinear speed-persistence correlation (nonlinear RWSP). The coarse-grained analysis showed that the nonlinear RWSP achieved the largest motility in both homogeneous and porous environments. When a particle searches for targets, the nonlinear coupling of speed and persistence improves the search ability (i.e. find more targets in a fixed time period), but sacrifices the search efficiency (i.e. find less targets per unit distance). Moreover, both the convex and concave pores restrict particle motion, especially for the nonlinear RWSP and Lévy walk. Overall, our results demonstrate that the nonlinear correlation of speed and persistence has the potential to enhance the motility and searching properties in complex environments, and could serve as a starting point for more detailed studies of active particles in biological, engineering and social science fields.

scholarly journals The Impact of Elastic Deformations of the Extracellular Matrix on Cell Migration

2020 ◽  
Vol 82 (4) ◽  
Author(s):  
A. A. Malik ◽  
B. Wennberg ◽  
P. Gerlee
Keyword(s):  
Extracellular Matrix ◽  
Cell Migration ◽  
Human Cancer ◽  
Matrix Stiffness ◽  
Human Cancer Cells ◽  
Adhesion Sites ◽  
The Matrix ◽  
A Cell ◽  
Necessary And Sufficient ◽  
The Impact

Abstract The mechanical properties of the extracellular matrix, in particular its stiffness, are known to impact cell migration. In this paper, we develop a mathematical model of a single cell migrating on an elastic matrix, which accounts for the deformation of the matrix induced by forces exerted by the cell, and investigate how the stiffness impacts the direction and speed of migration. We model a cell in 1D as a nucleus connected to a number of adhesion sites through elastic springs. The cell migrates by randomly updating the position of its adhesion sites. We start by investigating the case where the cell springs are constant, and then go on to assuming that they depend on the matrix stiffness, on matrices of both uniform stiffness as well as those with a stiffness gradient. We find that the assumption that cell springs depend on the substrate stiffness is necessary and sufficient for an efficient durotactic response. We compare simulations to recent experimental observations of human cancer cells exhibiting durotaxis, which show good qualitative agreement.

scholarly journals Phosphodiesterase SMPDL3B Gene Expression as Independent Outcome Prediction Marker in Localized Prostate Cancer

2020 ◽  
Vol 21 (12) ◽  
pp. 4373
Author(s):  
Frank Waldbillig ◽  
Katja Nitschke ◽  
Abdallah Abdelhadi ◽  
Jost von Hardenberg ◽  
Philipp Nuhn ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Migration ◽  
Outcome Prediction ◽  
The Cancer Genome Atlas ◽  
Localized Prostate Cancer ◽  
Prostate Hyperplasia ◽  
Long Term Follow Up ◽  
And Migration ◽  
Sirna Knockdown ◽  
The Impact

Current outcome prediction markers for localized prostate cancer (PCa) are insufficient. The impact of the lipid-modifying Sphingomyelin Phosphodiesterase Acid Like 3B (SMPDL3B) in PCa is unknown. Two cohorts of patients with PCa who underwent radical prostatectomy (n = 40, n = 56) and benign prostate hyperplasia (BPH) controls (n = 8, n = 11) were profiled for SMPDL3B expression with qRT-PCR. Publicly available PCa cohorts (Memorial Sloane Kettering Cancer Centre (MSKCC; n = 131, n = 29 controls) and The Cancer Genome Atlas (TCGA; n = 497, n = 53 controls)) served for validation. SMPDL3B’s impact on proliferation and migration was analyzed in PC3 cells by siRNA knockdown. In both cohorts, a Gleason score and T stage independent significant overexpression of SMPDL3B was seen in PCa compared to BPH (p < 0.001 each). A lower expression of SMPDL3B was associated with a shorter overall survival (OS) (p = 0.005) in long term follow-up. A SMPDL3B overexpression in PCa tissue was confirmed in the validation cohorts (p < 0.001 each). In the TCGA patients with low SMPDL3B expression, biochemical recurrence-free survival (p = 0.011) and progression-free interval (p < 0.001) were shorter. Knockdown of SMPDL3B impaired PC3 cell migration but not proliferation (p = 0.0081). In summary, SMPLD3B is highly overexpressed in PCa tissue, is inversely associated with localized PCa prognosis, and impairs PCa cell migration.

LGL1 modulates proliferation, apoptosis, and migration of human fetal lung fibroblasts

2015 ◽  
Vol 308 (4) ◽  
pp. L391-L402 ◽  
Author(s):  
Hui Zhang ◽  
Neil B. Sweezey ◽  
Feige Kaplan
Keyword(s):  
Cell Migration ◽  
Epithelial Cells ◽  
Conditioned Medium ◽  
Fetal Lung ◽  
Late Gestation ◽  
Lung Fibroblasts ◽  
And Migration ◽  
The Impact ◽  
Human Fetal Lung ◽  
Lung Branching

Rapid growth and formation of new gas exchange units (alveogenesis) are hallmarks of the perinatal lung. Bronchopulmonary dysplasia (BPD), common in very premature infants, is characterized by premature arrest of alveogenesis. Mesenchymal cells (fibroblasts) regulate both lung branching and alveogenesis through mesenchymal-epithelial interactions. Temporal or spatial deficiency of late-gestation lung 1/cysteine-rich secretory protein LD2 (LGL1/CRISPLD2), expressed in and secreted by lung fibroblasts, can impair both lung branching and alveogenesis (LGL1 denotes late gestation lung 1 protein; LGL1 denotes the human gene; Lgl1 denotes the mouse/rat gene). Absence of Lgl1 is embryonic lethal. Lgl1 levels are dramatically reduced in oxygen toxicity rat models of BPD, and heterozygous Lgl1+/−mice exhibit features resembling human BPD. To explore the role of LGL1 in mesenchymal-epithelial interactions in developing lung, we developed a doxycycline (DOX)-inducible RNA-mediated LGL1 knockdown cellular model in human fetal lung fibroblasts (MRC5LGL1KD). We assessed the impact of LGL1 on cell proliferation, cell migration, apoptosis, and wound healing. DOX-induced MRC5LGL1KDsuppressed cell growth and increased apoptosis of annexin V+staining cells and caspase 3/7 activity. LGL1-conditioned medium increased migration of fetal rat primary lung epithelial cells and human airway epithelial cells. Impaired healing by MRC5LGL1KDcells of a wound model was attenuated by addition of LGL1-conditioned medium. Suppression of LGL1 was associated with dysregulation of extracellular matrix genes (downregulated MMP1, ColXVα1, and ELASTIN) and proapoptosis genes (upregulated BAD, BAK, CASP2, and TNFRSF1B) and inhibition of 44/42MAPK phosphorylation. Our findings define a role for LGL1 in fibroblast expansion and migration, epithelial cell migration, and mesenchymal-epithelial signaling, key processes in fetal lung development.

Cell Self Assembly of Intracellular Interface Using Cell Migration

2008 ◽  
Vol 1092 ◽  
Author(s):  
Takayuki Hoshino ◽  
Tomohiro Konno ◽  
Kazuhiko Ishihara ◽  
Keisuke Morishima
Keyword(s):  
Cell Migration ◽  
Self Assembly ◽  
Focused Ion Beam ◽  
Adhesion Force ◽  
Ion Beam ◽  
Glass Plate ◽  
Electrode Arrays ◽  
A Cell ◽  
And Migration ◽  
Hybrid Interface

AbstractA cell-driven self-assembly of intracellular nano-device was proposed for bio-hybrid interface. This cells-driven self-assembly employed cell migration force to insert a conductive nanoneedle which would be worked as intracellular electrode. Such a nanoneedle was fabricated in the bottom of a microwell using focused ion beam induce deposition. The microwell structure with a coating of cell adhesion molecules was employed as the scaffold of the cell migration. A glass plate with the microwells had a non cell binding coating of 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer as an anti-biofouling material. Thus a cell adhered only on the wall of a microwell then the cell migrated into the microwell. Adhesion force and migration force induced self-insertion of the nanoneedle into a live cell body using the cell's own migration force. The inserted nanoneedle was made of electrical conductive tungsten, so the intracellular nanoneedle might extract intracellular potential more precisely than extracellular electrode, while inducing much less damage to cells. In the future, the technique of cell-driven self-insertion of nanoneedle may be integrated with multi electrode arrays for developing long-lasting measurements device on cellular network researches, or the risk assessment of the nanomaterials on cellular activities.

Syrian Crisis and Migration

2015 ◽  
Vol 12 (3) ◽  
pp. 181-192 ◽  
Author(s):  
Pinar Yazgan ◽  
Deniz Eroglu Utku ◽  
Ibrahim Sirkeci
Keyword(s):  
Human Mobility ◽  
Special Issue ◽  
Host Countries ◽  
Syrian Crisis ◽  
Mass Migration ◽  
Large Numbers ◽  
And Migration ◽  
Europe Today ◽  
The Impact ◽  
Near Future

With the growing insurrections in Syria in 2011, an exodus in large numbers have emerged. The turmoil and violence have caused mass migration to destinations both within the region and beyond. The current "refugee crisis" has escalated sharply and its impact is widening from neighbouring countries toward Europe. Today, the Syrian crisis is the major cause for an increase in displacement and the resultant dire humanitarian situation in the region. Since the conflict shows no signs of abating in the near future, there is a constant increase in the number of Syrians fleeing their homes. However, questions on the future impact of the Syrian crisis on the scope and scale of this human mobility are still to be answered. As the impact of the Syrian crisis on host countries increases, so does the demand for the analyses of the needs for development and protection in these countries. In this special issue, we aim to bring together a number of studies examining and discussing human mobility in relation to the Syrian crisis.

Race, Gender, and Border Control in the Western Balkans

2018 ◽  
Author(s):  
Sanja Milivojević
Keyword(s):  
Knowledge Production ◽  
Asylum Seekers ◽  
Former Yugoslavia ◽  
Border Control ◽  
Western Balkans ◽  
Irregular Migrants ◽  
Criminological Inquiry ◽  
Gender And Migration ◽  
And Migration ◽  
The Impact

This chapter looks at the intersection of race, gender, and migration in the Western Balkans. Immobilizing mobile bodies from the Global South has increasingly been the focus of criminological inquiry. Such inquiry, however, has largely excluded the Western Balkans. A difficult place to research, comprising countries of the former Yugoslavia and Albania, the region is the second-largest route for irregular migrants in Europe (Frontex 2016). Indeed, EU expansion and global developments such as wars in Syria, Afghanistan, and Iraq have had a major impact on mobility and migration in the region. The chapter outlines racialized hierarchies in play in contemporary border policing in the region, and how these racialized and gendered practices target racially different Others and women irregular migrants and asylum seekers. Finally, this chapter maps the impact of such practices and calls for a shift in knowledge production in documenting and addressing such discriminatory practices.

scholarly journals Cyclooxygenase Inhibition Alters Proliferative, Migratory, and Invasive Properties of Human Glioblastoma Cells In Vitro

2021 ◽  
Vol 22 (9) ◽  
pp. 4297
Author(s):  
Matthew Thomas Ferreira ◽  
Juliano Andreoli Miyake ◽  
Renata Nascimento Gomes ◽  
Fábio Feitoza ◽  
Pollyana Bulgarelli Stevannato ◽  
...  
Keyword(s):  
Cell Migration ◽  
Cell Biology ◽  
Gelatin Zymography ◽  
Viable Cell ◽  
Cell Counting ◽  
Gelatinolytic Activity ◽  
Ep4 Receptor ◽  
And Migration ◽  
Proliferation And Migration

Prostaglandin E2 (PGE2) is known to increase glioblastoma (GBM) cell proliferation and migration while cyclooxygenase (COX) inhibition decreases proliferation and migration. The present study investigated the effects of COX inhibitors and PGE2 receptor antagonists on GBM cell biology. Cells were grown with inhibitors and dose response, viable cell counting, flow cytometry, cell migration, gene expression, Western blotting, and gelatin zymography studies were performed. The stimulatory effects of PGE2 and the inhibitory effects of ibuprofen (IBP) were confirmed in GBM cells. The EP2 and EP4 receptors were identified as important mediators of the actions of PGE2 in GBM cells. The concomitant inhibition of EP2 and EP4 caused a significant decrease in cell migration which was not reverted by exogenous PGE2. In T98G cells exogenous PGE2 increased latent MMP2 gelatinolytic activity. The inhibition of COX1 or COX2 caused significant alterations in MMP2 expression and gelatinolytic activity in GBM cells. These findings provide further evidence for the importance of PGE2 signalling through the EP2 and the EP4 receptor in the control of GBM cell biology. They also support the hypothesis that a relationship exists between COX1 and MMP2 in GBM cells which merits further investigation as a novel therapeutic target for drug development.

scholarly journals MiR-188-3p and miR-133b Suppress Cell Proliferation in Human Hepatocellular Carcinoma via Post-Transcriptional Suppression of NDRG1

2021 ◽  
Vol 20 ◽  
pp. 153303382110330
Author(s):  
Zhenzhao Luo ◽  
Yue Fan ◽  
Xianchang Liu ◽  
Shuiyi Liu ◽  
Xiaoyu Kong ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Proliferation ◽  
Cell Migration ◽  
Cell Growth ◽  
Suppressive Effect ◽  
Luciferase Reporter ◽  
Invasion And Migration ◽  
And Migration ◽  
Cancer Tissues ◽  
Suppress Cell Proliferation

Background: Previous studies reported that N-myc downstream-regulated gene 1 (NDRG1) was upregulated in various cancer tissues and decreased expression of miR-188-3p and miR-133b could suppress cell proliferation, metastasis, and invasion and induce apoptosis of cancer cells. However, the molecular mechanism of NRDG1 involved in hepatocellular carcinoma (HCC) tumorigenesis is still unknown. Methods: The expressions of miR-188-3p, miR-133b, and NRDG1 in HCC tissues and cells were quantified by qRT-PCR and Western blot. MTT assay and transwell invasion assay were performed to evaluate cell growth and cell migration, respectively. Luciferase reporter assay were performed to determine whether miR-188-3p and miR-133b could directly bind to NRDG1 in HCC cells. Results: The results showed that NRDG1 was upregulated and these 2 microRNAs were downregulated in HCC tissues. NRDG1 was negatively correlated with miR-188-3p and miR-133b in HCC tissues. MiR-188-3p and miR-133b were demonstrated to directly bind to 3′UTR of NRDG1 and inhibit its expression. Upregulation of miR-188-3p and miR-133b reduced NRDG1 expression in hepatocellular carcinoma cell lines, which consequently inhibited cell growth and cell migration. Conclusions: Our finding suggested that miR-188-3p and miR-133b exert a suppressive effect on hepatocellular carcinoma proliferation, invasion, and migration through downregulation of NDRG1.

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