Accumulation of 99mTc-Cysteine in Experimental Inflammatory Lesions in Comparison to 99mTc-Glutamine and 99mTc-HIG

1997 ◽  
Vol 36 (08) ◽  
pp. 265-269
Author(s):  
N. C. M. Gülaldi ◽  
U. Uysal ◽  
S. Ünsal ◽  
M. Aydin ◽  
İ. Peksoy ◽  
...  
Keyword(s):  
Gamma Camera ◽  
Lower Cost ◽  
Preparation Method ◽  
Tissue Concentration ◽  
Inflammatory Lesions ◽  
Promising Agent ◽  
Lower Blood ◽  
Arthritic Joints ◽  
Gamma Counter ◽  
Concentration Ratios

Summary Aim: The aim of this experimental work was to investigate the efficacy of 99mTc-L-Cysteine (Cys) in scintigraphic visualization of inflammatory lesions in comparison to 99mTc-L-glutamine (G), and 99mTc-HIG. Methods: In mice abscesses were induced by intramuscular injection of turpentine. Six days later mice were injected with 3.7 MBq of each agent and sacrificed in groups of three at 1, 3, 6 and 24 h. Scintigrams were obtained with a gamma camera. The organs, some blood, abscesses, some muscle and urine were removed, weighed and counted in a gamma counter. Percentage of uptake by organs and per gram tissues and abscess/normal tissue concentration ratios were calculated. Experimental arthritis was produced in 6 New Zealand rabbits by intraarticular injection of ovalbumin. Four days later 37 MBq of 99mTc-Cys and 99mTc-HIG were each i.v. administered to 3 rabbits. Scintigrams obtained at 1, 3, 6, and 24 h demonstrated the arthritic joints very well. ROI’s over arthritic joints were compared to contralateral normal joints (A/C). Results: In mice the abscesses were well visualized on all scintigrams. The maximum abscess/muscle ratios were 5.21 ±1.09 (6 h), 3.73 ± 0.81 (3 h) and 5.98 ± 1.17 (24 h) and the maximum abscess/blood ratios were 3.46 ± 1.33 (24 h), 1.81 ± 0.10 (6 h) and 0.914 ± 0.351 (24 h) for 99mTc-Cys, 99mTc-G, and 99mTc-HIG, respectively. In rabbits the maximum A/C ratios were 2.61 ± 0.53 (3 h) and 2.92 ± 0.99 (24 h) for 99mTc-Cys and 99mTc-HIG, respectively. Conclusion: our results indicate that 99mTc-Cys is a promising agent for imaging inflammatory lesions. It is preferred to 99mTc-HIG, because of higher concentration ratios attained earlier, lower blood background, lower cost and a simpler in-house preparation method.

Whole-body to tissue concentration ratios for use in biota dose assessments for animals

2010 ◽  
Vol 49 (4) ◽  
pp. 549-565 ◽  
Author(s):  
Tamara L. Yankovich ◽  
Nicholas A. Beresford ◽  
Michael D. Wood ◽  
Tasuo Aono ◽  
Pål Andersson ◽  
...  
Keyword(s):  
Tissue Concentration ◽  
Whole Body ◽  
Dose Assessments ◽  
Concentration Ratios

Technetium-99m sulfur colloid (TSC) as a phenotypic probe for predicting pharmacokinetics (PK) and palmar-plantar erythrodysesthesia (PPE) toxicity of pegylated liposomal doxorubicin (PLD) in patients (pts) with recurrent epithelial ovarian cancer (EOC).

2012 ◽  
Vol 30 (15_suppl) ◽  
pp. 5050-5050 ◽  
Author(s):  
Hugh Giovinazzo ◽  
Parag Kumar ◽  
Arif Sheikh ◽  
Marija Ivanovic ◽  
Mark Doughty Walsh ◽  
...  
Keyword(s):  
Gamma Camera ◽  
Positive Relationship ◽  
Liposomal Doxorubicin ◽  
Solid Phase ◽  
Pegylated Liposomal Doxorubicin ◽  
Compartmental Analysis ◽  
Mononuclear Phagocyte ◽  
Sulfur Colloid ◽  
Spearman’S Correlation ◽  
Gamma Counter

5050 Background: There is significant variability in the PK and PD (efficacy and toxicity) of PLD. Clearance (CL) of PLD has been proposed to occur primarily via uptake by cells of the mononuclear phagocyte system (MPS) mainly located in the liver, spleen, and blood. TSC is a radioactive colloid used clinically for diagnostic and functional imaging of the MPS. Our aim is to evaluate TSC as a phenotypic probe of MPS activity, which may predict PLD PK and PPE toxicity in pts (n=9) with EOC. Methods: Prior to administration of PLD on cycle 1 day 1 (C1D1), TSC was administered at 10 mCi IVP × 1. Dynamic planar and SPECT/CT images of the liver, spleen and hands were acquired using a gamma camera. Blood samples were collected up to 60 min after TSC and analyzed for radioactivity using a gamma counter. On C1D1, PLD was administered at 40 mg/m2 alone or at 30 mg/m2 in combination with carboplatin IV over 1 to 3 h. Serial PK samples were obtained from 0h to 672h post PLD dose. Plasma was processed to measure encapsulated and released doxorubicin (dox) using solid phase separation and HPLC. CL of PLD and TSC were calculated by non-compartmental analysis. The grade of PPE toxicity was determined by NCI CTCAE (v4.03) criteria. Results: Nine patients have undergone TSC imaging and eight patients completed TSC blood PK and PLD plasma PK studies. There was a positive linear relationship between TSC CL and encapsulated dox CL (R2 = 0.61, p=0.02). When patients were subdivided, there was a stronger relationship between TSC CL and encapsulated dox CL (R2 = 0.81, p=0.03) in pts receiving PLD monotherapy. A positive relationship using Spearman’s correlation (ρ=0.84, p=0.006) was also found between maximum grade PPE toxicity developed and estimated AUC of encapsulated dox in hands [(TSC AUCBlood)/(TSC AUCHand)*Encapsulated Dox AUCPlasma]. Conclusions: These results suggest that TSC is a probe for MPS function and PLD PK and PD and may be used to individualize PLD therapy in pts with EOC. In addition, our findings suggest TSC may be able to predict the development of PPE in patients.

Spect imaging of the serotonin transporter using a novel ligand - 123-I mZIENT - in human subjects - a dosimetry and biodisortribution study

2011 ◽  
Vol 26 (S2) ◽  
pp. 935-935
Author(s):  
R. Krishnadas ◽  
A. Nicol ◽  
S. Champion ◽  
S. Pimlott ◽  
J. Stehouwer ◽  
...  
Keyword(s):  
Healthy Volunteers ◽  
Gamma Camera ◽  
Human Subjects ◽  
The Body ◽  
Whole Body ◽  
Body Images ◽  
Promising Agent ◽  
Regional Brain ◽  
The Brain ◽  
Spect Images

Levels of serotonin in the body are regulated by the serotonin transporters (SERT), which are predominantly located on the presynaptic terminals of serotonin-containing neurons. Alterations in the density of SERT have been implicated in the pathophysiology of many neuropsychiatric disorders.AimTo evaluate 123-I mZIENT (2(S)-[(S)-2b-carbomethoxy-3b-[3′-((Z)-2-iodoethenyl)phenyl]nortropane), a novel radiopharmaceutical for imaging SERT. The bio-distribution of the radiopharmaceutical in humans was investigated and dosimetry performed.MethodsThe study includes three healthy volunteers and three patients receiving SSRIs. Whole body images obtained on a gamma camera at 10 minutes, 1, 2, 3, 6, 24 and 48 hours post administration. Dosimetry was performed. ROIs were drawn over the brain, heart, kidneys, liver, lungs, salivary glands, spleen, thyroid and intestines. Blood was sampled at 5, 15, & 30 minutes and 1, 2, 3, 6, 24 and 48 hours post administration. Urine was collected at 1, 2, 3, 4, 6, 24 and 48 hours. Brain SPECT images were obtained using a neuroSPECT scanner at 4 hours, evaluated visually and analysed using ROI analysis.ResultsHigh quality SPECT images can be obtained after 100–150 MBq 123-ImZEINT. Regional brain uptake was observed in midbrain and basal ganglia in healthy volunteers, consistent with the known distribution of SERT. Biodistribution images demonstrated highest uptake in the lungs, brain, liver and intestines. The effective dose was within range of other commonly used ligands and is acceptable for clinical imaging.Conclusion123-ImZIENT is a promising agent for imaging SERT in humans with acceptable dosimetry.

Research On Method of Discovering Second Phase Particles with Scanning Electron Microscope

2011 ◽  
Vol 287-290 ◽  
pp. 1061-1064
Author(s):  
Xue Gang Ma ◽  
Rong Li Sang ◽  
Yuan Liang Li ◽  
Yi Shen
Keyword(s):  
Electron Microscope ◽  
Chemical Composition ◽  
High Resolution ◽  
Scanning Electron Microscope ◽  
Lower Cost ◽  
Preparation Method ◽  
Second Phase ◽  
Second Phase Particles ◽  
Tem Method ◽  
Scanning Electron

The microphotograph, size distribution and chemical composition of second phase particles in Q195 steel were characterizated using SEM and EDS. In order to observe the nanometer grade second phase particles, SEM with high resolution should be used. Whether can be second phase particles discovered lies on the preparation method of samples. This method is easier for sample preparation, lower cost, more efficient than TEM method.

scholarly journals Comparison of three methods used for measurement of radioiodine fixation in thyroid gland of mice

2013 ◽  
Vol 28 (2) ◽  
pp. 225-231 ◽  
Author(s):  
Marija Jeremic ◽  
Milovan Matovic ◽  
Slobodan Jankovic ◽  
Milorad Milosev ◽  
Milan Novakovic ◽  
...  
Keyword(s):  
Thyroid Gland ◽  
Correlation Coefficient ◽  
Gamma Camera ◽  
Test Tube ◽  
Test Reliability ◽  
Fixation Rate ◽  
Radioiodine Uptake ◽  
Significant Difference ◽  
Gamma Counter

The aim of this work is to compare the results of estimation of radioiodine uptake using three methods in a study on mice, and to test reliability of the radioiodine uptake estimation by gamma camera. The study is conducted on 21 white, Swiss-type mice of both sex at age of 10 weeks, weighing between 25 g and 34 g. The mice were injected intraperitoneally with 0.37 ? ? 0.03 MBq of radioiodine 131I. After 72 hours the mice were anesthetized, and radioactivity of thyroid region was measured by gamma camera (the 1st method, in situ). After the measurement, the animals were sacrificed, their thyroid glands were carefully excised together with adjacent trachea and placed at the bottom of a test tube. The radioactivity of the excised tissue was then measured by both gamma camera (the 2nd method) and gamma counter (the 3rd method). This method is treated as a standard and the most accurate. In the study we used Siemens e_cam gamma camera and Wallac Wizard 1470 Automatic Gamma counter. The radioiodine fixation determined using those three methods was 25.25 ? 7.32%, 26.08 ? ? 8.55% and 25.74 ? 7.18%, without statisticaly significant difference s between methods (p > 0.05). The high correlation between the three methods of measuring radioiodine fixation in thyroid gland was observed: (1) the correlation coefficient between the fixation rate obtained by gamma camera in situ and the fixation rate obtained by measuring the radioactivity of extirpated thyroids by gamma camera was 0.869 (p < 0.01); (2) the correlation coefficient between fixation rate obtained by gamma camera in situ and the fixation rate obtained by measuring radioactivity of extirpated thyroids by gamma counter was 0.890 (p < 0.01); (3) the correlation coefficient between fixation rate obtained by measuring radioactivity of extirpated thyroids by gamma camera and the fixation rate obtained by measuring radioactivity of extirpated thyroids by gamma counter was 0.835 (p < 0.01).

Whole organism to tissue concentration ratios derived from an Australian tropical dataset

2018 ◽  
Vol 189 ◽  
pp. 31-39 ◽  
Author(s):  
Che Doering ◽  
Peter Medley ◽  
Blake Orr ◽  
David Urban
Keyword(s):  
Tissue Concentration ◽  
Concentration Ratios

The Possibilities of Scintigraphic Visualization of Surfactants in the Lung of the Rat

1980 ◽  
Vol 19 (01) ◽  
pp. 33-36
Author(s):  
E. Koren ◽  
K. Filjak ◽  
A. Gadže ◽  
Š. Spaventi
Keyword(s):  
Oleic Acid ◽  
Gamma Camera ◽  
Radioactive Iodine ◽  
The Body ◽  
Rat Lung ◽  
Time Intervals ◽  
Surfactant System ◽  
Metabolic Functions ◽  
Surfactant Phospholipids ◽  
Gamma Counter

Incorporation of radioactive iodine into the surfactant system of the rat lung has been studied. Specially prepared particles labelled by 125I-oleic acid or 131I-oleic acid were injected intravenously and the distribution of radioiodine in the body of the animals was observed at various time intervals by means of a gamma camera and a gamma counter. The results showing appreciable accumulation of isotope in the lungs and the surfactant phospholipids, constitute a basis for the development of a new scintigraphic method for studying metabolic functions and morphology of the lung.

Direct Observation of Heat Exchanger Deposits by Cross Sectioning

1967 ◽  
Vol 25 ◽  
pp. 364-365
Author(s):  
R. W. Anderson ◽  
D. L. Senecal
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Heat Exchanger ◽  
Direct Observation ◽  
Cross Sections ◽  
Preparation Method ◽  
Specimen Preparation ◽  
Flowing Water ◽  
Transmission Electron ◽  
Deposit Layer

A problem was presented to observe the packing densities of deposits of sub-micron corrosion product particles. The deposits were 5-100 mils thick and had formed on the inside surfaces of 3/8 inch diameter Zircaloy-2 heat exchanger tubes. The particles were iron oxides deposited from flowing water and consequently were only weakly bonded. Particular care was required during handling to preserve the original formations of the deposits. The specimen preparation method described below allowed direct observation of cross sections of the deposit layers by transmission electron microscopy.The specimens were short sections of the tubes (about 3 inches long) that were carefully cut from the systems. The insides of the tube sections were first coated with a thin layer of a fluid epoxy resin by dipping. This coating served to impregnate the deposit layer as well as to protect the layer if subsequent handling were required.

Use of Protein a Conjugated to Peroxidase to Localize Immunoglobulin G in SSPE Ferret Brain

1982 ◽  
Vol 40 ◽  
pp. 350-351
Author(s):  
Hannah R. Brown ◽  
Anthony F. Nostro ◽  
Halldor Thormar
Keyword(s):  
Immunoglobulin G ◽  
Human Disease ◽  
Measles Virus ◽  
Subacute Sclerosing Panencephalitis ◽  
Protein A ◽  
Inflammatory Lesions ◽  
Sspe Virus ◽  
Specific Immunoglobulin ◽  
Antibody Titers ◽  
The Brain

Subacute sclerosing panencephalitis (SSPE) is a slowly progressing disease of the CNS in children which is caused by measles virus. Ferrets immunized with measles virus prior to inoculation with the cell associated, syncytiogenic D.R. strain of SSPE virus exhibit characteristics very similar to the human disease. Measles virus nucleocapsids are present, high measles antibody titers are found in the sera and inflammatory lesions are prominent in the brains. Measles virus specific immunoglobulin G (IgG) is present in the brain,and IgG/ albumin ratios indicate that the antibodies are synthesized within the CNS.

Sign in / Sign up

Export Citation Format

Share Document