scholarly journals Antibacterial activity test of the partially purified bromelain from pineapple core extract (Ananas comosus [L.] Merr) by fractionation using ammonium sulfate acetone

2018 ◽  
Author(s):  
Y. Hidayat ◽  
E. Hermawati ◽  
S. Setiasih ◽  
S. Hudiyono ◽  
E. Saepudin
Keyword(s):  
Antibacterial Activity ◽  
Ammonium Sulfate ◽  
Ananas Comosus ◽  
Activity Test

scholarly journals SOLVENT FREE SYNTHESIS OF CHALCONE AS ANTIBACTERIAL SUBSTANCE

2017 ◽  
Vol 13 (1) ◽  
pp. 95
Author(s):  
Susy Yunita Prabawati ◽  
Arifah Khusnuryani ◽  
Khamidinal Khamidinal
Keyword(s):  
Antibacterial Activity ◽  
Solvent Free ◽  
Hydration Reaction ◽  
Antibacterial Substance ◽  
E Coli ◽  
H Nmr ◽  
Activity Test ◽  
Grinding Technique ◽  
Solvent Free Synthesis

<p>The aims of this study was to synthesize a compound of 3-methoxy-4-hydroxychalcone through Claisen-Schmidt condensation with grinding technique (solvent-free). Vanillin, acetophenone and 60 % NaOH catalyst used in this synthesis. Characterization of products was done with a spectrophotometer FTIR and <sup>1</sup>H-NMR spectrometer. The  product was obtained as a orange solid which has a melting point at    58 - 59 ˚C. Identification of the product by IR spectrophotometer showed the absorption of  C=C in the wavenumber of 1496.76 cm<sup>-1</sup> which confirmed that the hydration reaction of chalcone had occurred. Analysis using <sup>1</sup>H-NMR spectrometer also showed the proton of the CH=CH appearing on chemical shift (δ) 7.99 ppm. The antibacterial activity test showed that the compounds of 3-methoxy-4- hydroxychalcone has a potential as an antibacterial against bacteria <em>E. coli</em> and <em>B. subtilis</em>.</p>

scholarly journals ISOLATION OF ACTINOMYCIN D FROM MARINE DERIVED Streptomyces

2010 ◽  
Vol 7 (3) ◽  
pp. 350-353
Author(s):  
Abdullah Rasyid ◽  
Kyoko Adachi
Keyword(s):  
Antibacterial Activity ◽  
Crude Extract ◽  
Marine Bacterium ◽  
Actinomycin D ◽  
Culture Supernatant ◽  
Culture Broth ◽  
Bacterial Cells ◽  
Antibacterial Screening ◽  
Activity Test ◽  
Bioactive Substance

The bioactive substance producing strain, a marine bacterium A5S-46 was found in the antibacterial screening. This strain was isolated from the artificial sponge set in the coastal seawater at Iriomote islands (Japan). The crude extract from culture broth of the strain A5S-46 was assayed to the antibacterial activity test against the seven kinds of bacterial test strains. The active substance accumulated in the both bacterial cells and culture supernatant. Based on the 1H NMR and the LC/PDA/MS data, the bioactive substance was identified to be actinomycin D.   Keywords: Marine bacteria, Actinomycin D, Antibacterial, Anticancer

scholarly journals Analisis Pigmen dan Aktivitas Antibakteri In Vitro Pigmen Astaksantin Kepiting (Grapsus albolineatus Lamarck) Jantan

2006 ◽  
Vol 8 (2) ◽  
pp. 160
Author(s):  
Aswan Thamin ◽  
Chairulwan Umar ◽  
Darussadah Paransa
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Enterobacter Cloacae ◽  
Bacteriostatic Activity ◽  
North Sulawesi ◽  
Marine Crustaceans ◽  
Activity Test ◽  
In Vitro Antibacterial Activity

Grapsus albolineatus is one of marine crustaceans which have carotenoid (astaxanthin) pigment. This research was conducted to analyze carotenoids (astaxanthin) extracted from G. albolineatus, and evaluate their in vitro antibacterial activity. The research was done in March-July 2002. Samples were collected from Manado Gulf, North Sulawesi. The result indicated that the carapace contained 4 carotenoids namely ß-caroten, ecinenon, astaxanthin diester, and astaxanthin monoester. In addition, the epidermis contained free astaxanthin. In vitro antibacterial activity test indicated that astaxanthin had low bacteriostatic activity against Psedomonas aeruginosa, Enterobacter cloacae, Staphylococcus aureus, and Proteus stuartii.

scholarly journals Antibacterial Activity Test of Bacteriocin from Lactobacillus brevis, Lactobacillus casei and Lactobacillus plantarum Against Gram Positive Pathogenic Bacteria

2018 ◽  
Vol 3 (3) ◽  
pp. 85
Author(s):  
Novi Permata Sari ◽  
Rafika Sari ◽  
Eka Kartika Untari
Keyword(s):  
Antibacterial Activity ◽  
Lactobacillus Plantarum ◽  
Lactobacillus Casei ◽  
Pathogenic Bacteria ◽  
Heating Temperature ◽  
Lactobacillus Brevis ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Activity Test ◽  
Ph Range

Bacteriocin is a secondary metabolite product of lactic acid bacteria (LAB) which have an antimicrobial and potentially as a natural preservative. LAB isolates used in this study were Lactobacillus brevis, Lactobacillus casei and Lactobacillus plantarum. This study aimed to determine the antibacterial activity of bacteriocin produced by each isolate of LAB including the influence of pH and heating variation against Bacillus cereus, Bacillus subtilis and Staphylococcus epidermidis. Antibacterial activity test was done by using disc diffusion method. method. Confirmation test using proteolytic enzyme aimed to analyse that the inhibition zone produced from the activity of bacteriocin. The inhibition zone produced from L. brevis, L. casei and L. plantarum against B. cereus were 15.70, 16.43 and 14.50 mm, against B. subtilis were 13.37, 14.10 and 12.53 mm and against S. epidermidis were 11.37, 14.50 and 12.45 mm. The activity of each bacteriocin decreased with the addition of trypsin and catalase, bacteriocin was active in the pH range of 2-10 and heating temperature of 40-121oC. Statistical test showed that the addition of trypsin, catalase and the variation of pH also heating had significant differences (p<0.05) to antibacterial activity produced by bacteriocin from L. brevis, L. casei and L. plantarum. 

scholarly journals ANALYSIS OF SECONDARY METABOLITES, ANTIBACTERIAL ACTIVITY AND COMPOUND COMPOSITION IN THE SEA CUCUMBER Bohadschia sp. EXTRACT

2017 ◽  
Vol 8 (2) ◽  
pp. 645-653 ◽  
Author(s):  
Abdullah Rasyid
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Secondary Metabolites ◽  
Sea Cucumber ◽  
Similarity Index ◽  
Diffusion Method ◽  
Composition Analysis ◽  
Activity Test ◽  
Agar Diffusion Method ◽  
Color Reactions

Bohadschia sp. is one of the sea cucumber species that has potential to be developed as a source of antibacterial from the sea. Samples of sea cucumber Bohadschia sp. used in this study collected from the Ratai bay waters, Lampung. This study aims to determine the type of secondary metabolites, antibacterial activity and compound composition analysis containing in the sea cucumber extract. Identification of secondary metabolites by observation of color reactions, precipitation and foam. The method used to antibacterial activity test was the agar diffusion method, while identification of the composition of compounds performed with Gas Chromatogaphy-Mass Spectroscopy (GC-MS) method.Top of FormThe results showed that the type of secondary metabolites contained in the extract of sea cucumber Bohadschia sp. were steroids and saponins. The extract of sea cucumber Bohadschia sp. showed antibacterial activity against Bacillus subtilis and Vibrio eltor. Results of GC-MS were 12 compounds and have a similarity index same or more than 90%. All compounds consist of organosilicon cyclic, fatty acid, steroid, cyclo alkene and alkena. The compound with biggest abundance was cholest-5-en-3-yl nonanoate (4.89%) and retention time was 37.370 minutes.

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL 96% DAUN MIMBA (Azadirachta indica A. Juss. ) DENGAN METODE EKSTRAKSI PERKOLASI TERHADAP PERTUMBUHAN BAKTERI

2020 ◽  
Vol 2 (1) ◽  
pp. 102
Author(s):  
Yanu Andhiarto ◽  
Rina Andayani ◽  
Nur Hidayatul Ilmiyah
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Skin Inflammation ◽  
Screening Tests ◽  
Diffusion Method ◽  
Moderate Activity ◽  
Phytochemical Screening ◽  
Strong Activity ◽  
Activity Test

Skin infections most often found in developing countries, especially in the tropics, are purulent skin inflammation (pyoderma), the main cause of Staphylococcus aureus. The increasing number of cases of bacteria that are resistant to antibacterial can encourage the extraction of sources of antibacterial drugs from natural ingredients. One of the plants that is thought to have the antibacterial activity of Staphylococcus aureus is the Neem plant. In this study an antibacterial activity test was carried out with the well diffusion method from 96% ethanol extract of Mimba leaves extracted by percolation method. Previously 96% ethanol extract from Mimba leaves had been identified by secondary metabolites by phytochemical screening and standardized extract testing. Standarization using non-specific parameters (moisture content, ash content and drying losses) found that 96% ethanol extract of Mimba leaves entered a range of standard parameters and in phytochemical screening tests obtained 96% ethanol extract of Mimba leaves containing alcaloid, saponins, tannins, steroids / terpenoids and flavonoids. The results of the study on the antibacterial activity test showed a concentration of 75% ethanol extract of 96% Mimba leaves classified as having strong activity, at a concentration of 50% and 25% classified as having moderate activity. Based on this study it can be concluded that the three concentrations of 96% ethanol extract of Mimba leaves were able to inhibit the growth of Staphylococcus aureus bacteria.

Aktivitas Antibakteri dari Ekstrak Etanol Ganoderma sp. Asal Pulau Lombok

2020 ◽  
Vol 6 (1) ◽  
pp. 22
Author(s):  
Adelina Oktaviani ◽  
Aida Muspiah ◽  
Faturrahman Faturrahman
Keyword(s):  
Antibacterial Activity ◽  
Inhibitory Activity ◽  
Large Diameter ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Gram Negative Bacteria ◽  
Activity Test

The use of antibiotics that are not according to the rules and antibiotics in the long term can cause resistance to bacteria. This study aims to determine the presence of antibacterial activity and the effect of increasing the concentration of ethanol extract of Ganoderma sp. against several test bacteria. Extract from Ganoderma sp. obtained by maceration method using ethanol 95% solvent. The extract concentrations used were 20%, 40%, 60% and 80%. This research was conducted using the wells method with ciprofloxacin as a positive control and 50% DMSO as a negative control. The parameter measured is the large diameter of the inhibition formed around the well. The results of the antibacterial activity test of ethanol extract Ganoderma sp. has greater inhibitory activity against gram-negative bacteria. The inhibitory activity of ethanolic extract of Ganoderma sp. on the growth of test bacteria increased with increasing concentration of the extract

Green Synthesis of Silver Nanoparticles from Ananas comosus Core Extract and Their Antibacterial Activity

2020 ◽  
pp. 455-463
Author(s):  
Zainab Razali ◽  
Nur Wajihah Masdar ◽  
Nor Atikah Husna Ahmad Nasir ◽  
Nur Syafiqah Rahim ◽  
Roejhan Md Kawi
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Ananas Comosus
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