scholarly journals Taking essential oil by water distillation and antibacterial activity test of refined water from agarwood plant parts (Aquilaria malaccensis Lamk)

2021 ◽  
Vol 782 (3) ◽  
pp. 032076
Author(s):  
M Nurminah ◽  
R Batubara ◽  
T Ismanelly ◽  
Albert
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Water Distillation ◽  
Plant Parts ◽  
Activity Test ◽  
Aquilaria Malaccensis

scholarly journals Antibacterial Activities of Rough Lemon (Citrus jambhiri Lush) Rind Essential Oil

2019 ◽  
Vol 1 (1) ◽  
pp. 12-18
Author(s):  
Helmina Sembiring
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Chemical Compounds ◽  
Antibacterial Activities ◽  
Diffusion Test ◽  
Citrus Jambhiri ◽  
Disk Diffusion Test ◽  
Rough Lemon ◽  
Activity Test ◽  
Paper Disk

The isolation of rough lemon (Citrus jambhiri Lush) rind essential oil has been done by using hydrodistillation method and paper disk diffusion test for antibacterial activity test. 2.3 gram (1.51%) of essential oil was obtained from 450 gram of dried rough lemon rind powder. Based on the analysis using GC-MS, the essential oil from rough lemon rind contains 5 chemical compounds, but only 4 of them can be interpreted. They are Limonene (71.88%), 1,4-cyclohexadiene (13.93%), β-Pinene (10.57%) and β-Ocimene (2.37%). Antibacterial activity was tested to Bacillus cereus and Escherichia coli at 40% concentration (v/v) with 12.7 mm and 12.9 mm of clear zones respectively.

scholarly journals Aktivitas Antibakteri Minyak Atsiri Kulit Kayu Akway (Drimys piperita Hook. f.) pada Beberapa Tingkat Konsentrasi, Keasaman (pH) dan Kandungan Garam

2019 ◽  
Vol 8 (4) ◽  
pp. 149
Author(s):  
Gino Nemesio Cepeda ◽  
Meike Meilan Lisangan ◽  
Isak Silamba
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Sodium Chloride ◽  
Essential Oil ◽  
Bacillus Cereus ◽  
Chloride Content ◽  
Antibacterial Activities ◽  
Water Distillation

Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri minyak atsiri kulit kayu akway pada beberapa tingkat konsentrasi, keasaman (pH) dan kandungan sodium klorida. Minyak atsiri disuling dengan menggunakan metode distilasi air. Pengujian aktivitas antibakteri minyak atsiri pada beberapa tingkat konsentrasi, pH dan kandungan sodium klorida dilakukan dengan menggunakan metode difusi sumur. Hasil penelitian menunjukkan bahwa aktivitas antibakteri minyak atsiri kulit kayu akway cenderung meningkat dengan meningkatnya konsentrasi. Konsentrasi penghambatan minimum terhadap Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa dan Staphylococcus aureus adalah 0,28–0,56%. Tingkat keasaman dan kandungan sodium klorida tidak berpengaruh nyata terhadap aktivitas antibakteri minyak atsiri kulit kayu akway. Kesimpulannya, minyak atsiri kulit kayu akway berpotensi sebagai sumber antibakteri alami untuk menghambat pertumbuhan bakteri yang tahan terhadap antibiotik.Antibacterial Activity of Essential Oil of Akway (Drimys piperita Hook f.) Barks on Some Levels of Concentration, Acidity (pH) and Salt ContentsAbstractAkway (Drimys piperita Hook. f) was an aromatic plant of winteraceae. Leaves and barks of this plant contain essential oil. Previous studies indicated that essential oil from some aromatic plants had strong antibacterial activities. The aims of the study were to know antibacterial activities of essential oil isolated from akway bark on some levels of concentration, acidity (pH) and sodium chloride content. The essential oil was distilled by using water distillation method. The antibacterial activity was assayed on several levels of concentration, pH and sodium chloride concentrations that were performed using method of agar well diffusion. The results showed that the antibacterial activity of akway barks essential oil tended to increase with increasing of concentrations. The minimum inhibition concentrations against Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa, and Staphylococcus aureus were 0,28–0,56%. The pH and sodium chloride contents had not significantly influenced to the antibacterial activities of akway barks essential oil. As conclusion, the essential oil of akway barks had potential as source of antibacterial on inhibiting growth of antibiotic resistance bacteria. 

scholarly journals Aktivitas Antibakteri Minyak Atsiri Kulit Jeruk Siam Banjar (Citrus reticulata) Terhadap Pertumbuhan Pseudomonas aeruginosa

2019 ◽  
Vol 6 (1) ◽  
pp. 10
Author(s):  
Dwi Rizki Febrianti ◽  
Yugo Susanto ◽  
Rakhmadhan Niah ◽  
Siti Latifah
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Orange Peel ◽  
Negative Control ◽  
Diffusion Method ◽  
Citrus Reticulata ◽  
Liquid Form ◽  
Activity Test

ABSTRAK Jeruk siam yang berkembang di Kalimantan Selatan telah dikukuhkan menjadi varietas unggul nasional dengan nama jeruk siam Banjar. Kulit jeruk belum dimanfaatkan secara optimal hanya dibuang sebagai limbah. Kulit jeruk mengandung beberapa senyawa salah satunya mengandung senyawa aktif minyak atsiri. Tujuan penelitian ini adalah untuk mengetahui adanya aktivitas antibakteri minyak atsiri kulit jeruk siam Banjar (Citrus reticulata) terhadap pertumbuhan Pseudomonas aeruginosa. metode penarikan minyak atsiri kulit jeruk siam Banjar menggunakan metode destilasi air. Uji aktivitas antibakteri dilakukan dengan menggunakan metode difusi cakram terhadap bakteri Pseudomonas aeruginosa. Hasil penelitian menunjukkan bahwa  minyak atsiri kulit jeruk siam Banjar diperoleh sebanyak 10 mL (0,58%), berwarna kuning, aroma khas jeruk, bentuk cair, rasa getir dan tidak ada noda transparan. Hasil penelitian uji aktivitas menunjukkan minyak atsiri kulit jeruk siam Banjar dapat menghambat pertumbuhan bakteri Pseudomonas aeruginosa. Pada volume 50 µL, 75 µL, dan 100 µL minyak atsiri kontrol positif (ciprofloxacin) sedangkan kontrol negatif (aqua pro injection). diameter zona bening disekitar cakam  dengan rata-rata diameter zona hambat yang dihasilkan beturut-turut 3,55 mm, 4,54 mm, 5,14 mm, 22,38 mm, dan 0 mm. Kata kunci: Kulit jeruk siam Banjar (Citrus reticulata), Minyak atsiri,  Pseudomonas aeruginosa. ABSTRACT Jeruk siam that develop in South Kalimantan have been confirmed as national superior varieties by the name of jeruk siam banjars. Citrus skin has not been used optimally only as waste. Orange peel contains several compounds, one of which contains active compounds of essential oils. The purpose of this study was to determine the antibacterial activity of essential oils of jerk siam banjars skin (Citrus reticulata) to the growth of Pseudomonas aeruginosa. method of withdrawal of jeruk siam banjars essential oil using a water distillation method. Antibacterial activity test was carried out using the disc diffusion method against the bacterium Pseudomonas aeruginosa. The results showed that 10 mL (0.58%) of essential oil from the jeruk siam banjars, yellow, orange aroma, liquid form, bitter taste, and no transparent stains. The results of the activity test showed that the essential oil of the skin of the jeruk siam banjars could inhibit the growth of the bacterium Pseudomonas aeruginosa. At the volume of 50 µL, 75 µL, and 100 µL of essential oil control positive (ciprofloxacin) while the negative control (aqua pro injection). the diameter of the clear zone around the paper disk with the average diameter of the inhibition zone produced was 3.55 mm, 4.54 mm, 5.14 mm, 22.38 mm and 0 mm respectively.Keywords: jeruk siam banjar (Citrus reticulata), essential oil, Pseudomonas aeruginosa.

scholarly journals UJI AKTIVITAS ANTIBAKTERI GEL MINYAK ATSIRI DAUN KEMANGI (Oscimum basillicum L) TERHADAP Staphylococcus aureus

2021 ◽  
Vol 2 (02) ◽  
pp. 15
Author(s):  
Fenita Shoviantari
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Diffusion Method ◽  
Homogeneity Test ◽  
Chemical Compositions ◽  
Adhesion Test ◽  
Disk Diffusion Method ◽  
Activity Test ◽  
Leaf Essential Oil ◽  
One Way Anova

The essential oil of basil leaves (Oscimum basillicum L) has a mixture of chemical compositions, the most common of which are terpenes, aldehydes, alcohols, esters, phenols and ketones. Of these compounds that have antibacterial activity is linalool (51.2-74.73%) which is included in the terpenoid compound group. Objective: This study aims to determine whether there is an effect of the concentration of basil essential oil on the physical quality test of gel preparations and to determine whether there is a difference in the effect of the concentration of basil essential oil on antibacterial activity. The essential oil of basil leaves essential oil hand antiseptic gel was formulated with 4%, 6%, and 8% concentration of basil essential oil. Method: Antibacterial activity test was performed using the Disk Diffusion method. Results: The results of the research for the hand antiseptic gel of the essential oil of basil leaves (Oscimum basillicum L) met the parameters of the organoleptic test, homogeneity test, pH test, spreadability test and adhesion test. Basil leaf essential oil hand antiseptic gel with a concentration of 4% can inhibit bacterial growth with an average of 9mm, a concentration of 6% with an average of 10mm, and a concentration of 8% with an average of 12mm. By analyzing the data using One Way ANOVA, the Sig. 0,000 which means that there are differences in the three formulations. Conclusion: The three formulations are susceptible to strong inhibition.

scholarly journals Uji Aktivitas Antibakteri Staphylococcus aureus dan Salmonella typhi dari Hasil Mikroenkapsulasi Minyak Atsiri Rogo (Premna Serratifolia Linn)

2020 ◽  
Vol 7 (2) ◽  
pp. 73
Author(s):  
Nursin Nursin ◽  
Laily Nurliana ◽  
Imran Imran ◽  
Rustam Musta
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Salmonella Typhi ◽  
Concentration Variation ◽  
Activity Test ◽  
The Difference ◽  
Inhibitory Power

Antibacterial activity test Staphylococcus aureus and Salmonella typhihas been performed by microencapsulation product of rogo essential oil (Premnaserratifolia Linn). This study aims to determine rogo oil activity test and microencapsulation results as antibacterial S. aureus and S. typhi. The results of antibacterial activity of rogo liquid oil to S. aureus and S. Typhi bacteria showed different inhibitory power of each concentration variation of 12.5%, 25%, 50% and 100% with 100% concentration as the best inhibitor for both bacteria. While the antibacterial activity test of S. aureus and S. typhi from microencapsulated rogo oil: maltodextrin showed the difference of each variation of concentration 1:10; 1:12; 1:14; 1:16 and 1:18 with a 1:14 composition of S. aureus and 1:18 in S. typhi as the best ratio of activity power. The antibacterial activity test between liquid rogo oil and microencapsulated result shows the difference, but it can be concluded that the inhibitory power of rogo oil from microencapsulation is more inhibited than 100% rogo oil.

scholarly journals Antibacterial Activity Test of Kaffir Lime (Citrus hystrix DC) Essential Oil Against Klebsiella pneumoniae ATCC

2017 ◽  
Vol 6 (2) ◽  
pp. 61-66
Author(s):  
Nasrullah Jamaluddin ◽  
◽  
Maimunah Hindun Pulungan ◽  
Warsito Warsito ◽  
◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Klebsiella Pneumoniae ◽  
Activity Test

scholarly journals Phytoconstituents and Biological Activities of Zanthoxylum armatum Fruit Extract

2021 ◽  
Vol 42 (1) ◽  
pp. 125-131
Author(s):  
Ishwor Pathak ◽  
Shusma Rokaha ◽  
Kiran Bahadur Bajracharya
Keyword(s):  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Biological Activities ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Zanthoxylum Armatum ◽  
Activity Test

The essential oil and methanol extract of the fruit of Zanthoxylum armatum DC were extracted by hydro-distillation in the Clevenger apparatus and cold percolation technique respectively and their chemical and biological studies were performed. The chemical compositions of essential oil were analyzed by GC-MS analysis. The most abundant ingredient was Linalool (75.31%) followed by E-methyl cinnamate (11.73%) and limonene (9.45%). The nature of the functional groups present in the extract was analyzed by performing FTIR analysis. The methanol extract showed the presence of alkaloids, flavonoids, glycosides, polyphenols, terpenoids, volatile oils, tannins, and saponins. Biological activities of the oil and extract were evaluated by performing brine shrimp bioassay, antibacterial activity test, and antifungal activity test.  Essential oil (LC50 = 76.70 µg/mL) and methanol extract (LC50 = 62.25 µg/mL) were found highly cytotoxic against brine-shrimp nauplii. The essential oil showed potent antibacterial activity against gram-positive bacteria Bacillus subtilis, Micrococcus leutus, and Staphylococcus aureus with the zone of inhibitions 11 mm, 16 mm, and 17 mm respectively and moderate antibacterial activity against the gram-negative bacteria Klebsiella pneumonia, Enterobacter cloacae, and Pseudomonas aeruginosa with the zone of inhibitions 9 mm, 5 mm and 6 mm respectively. The methanol extract was found to be effective against gram-positive bacteria only. Both oil and extract showed moderate antifungal activity against bacterial strains.

scholarly journals Uji Aktivitas Antibakteri Kombinasi Minyak Atsiri Biji Ketumbar (Coriandrum sativum L.) dan Gentamisin terhadap Staphylococcus epidermidis

2019 ◽  
Vol 6 (3) ◽  
pp. 421
Author(s):  
Bawon Triatmoko ◽  
Huda Almuttaqin ◽  
Dewi Dianasari
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Staphylococcus Epidermidis ◽  
Combination Effect ◽  
Antibacterial Efficacy ◽  
Activity Test ◽  
Single Use ◽  
Index Value ◽  
Mic Value ◽  
Better Than

  Gentamicin is one of the antibiotics from the aminoglycoside group used for the treatment of infections. Gentamicin has side effect such as permanent ototoxicity and reversible nephrotoxicity. It was necessary to reduce these effects by combining antibiotic with natural ingredients, one of those is coriander seeds essential oil. Combination therapy is done to reduce the potential side effects, and increase antibacterial efficacy. Antibacterial activity test were conducted to measure the MIC values of gentamicin, essential oils of coriander seeds, and combination of both. Based on the antibacterial activity test, MIC value of gentamicin was 4 µg/mL, coriander seed essential oil was 50 µg/mL and MIC combination of gentamicin and essential oil of coriander seeds were 0.5 µg/mL and 50 µg/mL. The FIC Index value obtained was 1.125 meaning that the combination effect was indifferent. Indifferent combination effect means the use of a combination of coriander seeds essential oil and gentamicin as antibacterial was not better than the single use.   Keywords: coriander, essential oil, antibacterial, FIC Index, S. epidermidis  

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