Nutritional evaluation of dried larvae and pupae meal of the housefly (Musca domestica) using chemical- and broiler-based biological assays

2014 ◽  
Vol 54 (3) ◽  
pp. 347 ◽  
Author(s):  
E. Pieterse ◽  
Q. Pretorius

The nutritional composition of common housefly (Musca domestica) larvae and pupae meal is reported in terms of proximate analysis, amino acid profile, fatty acid composition, apparent metabolisable energy and total tract digestibility (TTD) of nutrients. Proximate analysis and TTD of meal showed larvae and pupae meal to contain, on a DM basis, a gross energy value of 20.10 MJ/kg and 20.42 MJ/kg, respectively, and an apparent metabolisable energy value of 14.23 MJ/kg and 15.15 MJ/kg, respectively. Crude protein content was 60.38% and 76.23%, with TTDs of 69% and 79%, respectively, with similarly high values reported for individual amino acids. Amino acid analysis revealed a favourable amino acid composition with high lysine concentrations but marginally low methionine concentrations. Arginine : lysine ratios of larvae and pupae meal were 0.67 and 0.91, respectively, and isoleucine : leucine ratios were 0.68 and 0.64, respectively. Crude fat contents were 14.08% and 14.39%, with TTDs of 94% and 98%, respectively, and crude fibre contents were 8.59% and 15.71%, with TTDs of 62% and 58%. Housefly larvae meal had crude protein TTD of 69%, whereas that of pupae meal was 79%. Both larvae and pupae meal had high amino acid TTDs. The TTD values of the crude fat and crude fibre were determined at 94% and 62%, respectively, for the housefly larvae, and at 98% and 58%, respectively, for the housefly pupae. M. domestica larvae meal can therefore be regarded as a good-quality protein source suitable for animal feeding.

Author(s):  
D. O. Oshibanjo ◽  
O. O. Olusola ◽  
O. A. Ogunwole

Aims: The effect of cooking method and temperature on amino acid composition of breakfast sausage (BS) was undertaken in this study. Methodology: Three batches of prepared BS from beef, were randomly allotted to three cooking methods (CM): (boiling, grilling and frying) each at cooking temperatures (CT) of 80, 90 and 100°C to attain internal temperature of 72°C in a completely randomized design. Samples from each treatment were oven-dried and assayed for amino acid and proximate composition using standard procedures. Data obtained was analysed using descriptive statistic and ANOVA at α0.05. Results: Results showed that grilled sausage at 80°C had highest total amino acid profile (3.2%). Grilled sausage at 80°C had highest crude protein (25.58%). Grilled BS at 80°C recorded least fat content (15.99%). Grilled sausage at 80°C had the higher ash (6.66%) and least (1.40%) in boiled sausage at 90°C. Conclusion: Therefore, breakfast sausage could be best grilled at 80°C due to maintain high amino acid profile, crude protein, ash and lower fat content.


1955 ◽  
Vol 102 (4) ◽  
pp. 435-440 ◽  
Author(s):  
Leonard T. Skeggs ◽  
Walton H. Marsh ◽  
Joseph R. Kahn ◽  
Norman P. Shumway

A preparation of hypertensin I was purified by countercurrent distribution and was shown to migrate as a single component in starch blocks at pH 9.3 and 4.2. It had an isoelectric point of 7.7. Quantitative analysis by ion exchange column chromatography showed eight amino acids in approximately unimolar proportion: aspartic, proline, valine, isoleucine, leucine, tyrosine, phenylalanine, and arginine. There were in addition two moles of histidine.


2008 ◽  
Vol 14 (4) ◽  
pp. 325 ◽  
Author(s):  
S. PERTTILÄ ◽  
J. VALAJA ◽  
T. JALAVA

Using ileal digestible amino acids in feed optimising will intensify feed protein utilizing and decrease nitrogen excretion to the environment. The study determined the apparent ileal digestibility (AID) coefficients of amino acids in barley, wheat, oats, triticale, maize, and dehulled oats in the diets of 180 Ross broiler chickens (aged 24–35 days). The birds were fed semi-purified diets that contained grain as the sole protein source and chromium-mordanted straw as an indigestible marker. The AID coefficients of the nutrients were assessed using the slaughter technique, and the apparent metabolisable energy (AME) was determined using total excreta collection. The ileal digestibility of the dry matter and organic matter were the highest in maize. The AME of maize was higher than that of other cereals. The ileal digestibility of crude protein was higher in wheat than that in barley, oats and dehulled oats. The AME of wheat was similar to that of barley and oats but lower than that of triticale and dehulled oats. The amino acid AID was highest in wheat (0.86) and triticale (0.85) and lowest in oats (0.79) and barley 0.77). The average amino acid AID was 0.81 in dehulled oats. The threonine AID was the same in all tested ingredients. The lysine, methionine, and cystine AID coefficients were 0.81, 0.79, and 0.71 respectively for barley; 0.86, 0.84, and 0.38 respectively for oats; 0.87, 0.86, and 0.53 respectively for dehulled oats; 0.84, 0.90, and 0.66 respectively for maize; 0.89, 0.88, and 0.77 respectively for triticale; and 0.87, 0.85, and 0.71 respectively for wheat. Results indicated that AME –values of domestic grains (barley, oats and wheat) are in the same level. Especially, low AME value of wheat needs further investigation.;


Author(s):  
Oyetade Joshua Akinropo ◽  
Bello Lukman Abidemi ◽  
Adesiyan Blessing Adedayo

The proximate analysis of the fruit sample that homogenously sampled from the wild was taken and quartered to get appreciable weight fit for analysis. Due to it perishable nature the quartered sample for each of the fruit was stored in an air tight container and kept in the refrigerator at a temperature of about 4°C. For the determination of the nutritional composition, parameters which include their proximate, minerals, and vitamin C were quantitatively determined while the anti-nutrient composition were qualitatively and quantitatively analyzed. Compared to ackee’s apple, monkey cola was found to consist of 64.41% moisture content, 1.69% ash, 10.21% crude fibre, 1.25% crude fat, 4.44% crude fibre, 18.06% carbohydrates while ackee’s apple consist of 73.21% moisture, 1.49% ash, 9.38 % crude protein, 13.98% crude fat, 2.08% crude fibre, 0.86% carbohydrates and 4.45% of vitamin C. The mineral analysis for both samples was quantitatively determined using Atomic Absorption spectrophotometer (AAS). The minerals determined for ackee’s apple and monkey cola were magnesium which was 1391.65 ppm, calcium 628.23 ppm, sodium 506.96 ppm, potassium 3976.14 ppm, iron 1.0 ppm, copper 5.00, zinc 4.00, cobalt 3.0 and phosphorus 2616.90 ppm the iron however, was not detected. Ackee’s apple on the other hand, consist of magnesium 498.01 ppm, calcium 478.56 ppm, sodium 398.80 ppm, potassium 4970.18 ppm, copper 2.00, zinc 5.00, cobalt 3.0 and phosphorus 373.84 ppm. The qualitative screening of the anti-nutrients revealed the absence of phenol from both fruit samples while tannins were present only in the monkey cola. The flavonoids, phytic acid and oxalate were quantitatively determined to be 1240 mg, 625 mg and 155 mg for Monkey kola and 640 mg, 340 mg and 65 mg for Ackee’s apple.


2011 ◽  
Vol 75 (1) ◽  
pp. 57-69 ◽  
Author(s):  
Anna Kotlarz ◽  
Agnieszka Sujak ◽  
Wacław Strobel ◽  
Wilhelm Grzesiak

Chemical Composition and Nutritive Value of Protein of the Pea Seeds - Effect of Harvesting Year and VarietyWe tested nutritional usability of pea seeds (Pisum sativumL.) at full maturity. Four white-flowering and 5 colour-flowering new Polish cultivars were compared. We determined proximate composition of ground seeds collected over 4 years period as well as amino acids, minerals (Ca, P, Na, K), tannins and fiber fractions (NDF, ADF, ADL). The seeds contained between 224 and 260 g·kg-1of crude protein which was rich in Lys (6.8±0.8 g) but poor in Met+Cys (2.0±0.2 g·16 g-1N). The amount of tannins (as tannic acid equivalent) in white-flowering cvs was 4.3±0.9 g and in colour-flowering - 7.4±2.2 g. The calculated protein nutritional values were compared against amino acid standards of human and animal nutrition and the whole egg protein. Effects of the cropping year and variety variance in respect of chemical composition of seeds were examined. Crude protein, crude oil, N-free extractives, tannins, K, amino acid content: Leu, Phe+Tyr and Ala content were influenced significantly by the cultivar, while the cropping year had a significant influence on dry matter, crude ash, crude protein, crude fibre, crude oil, N-free extractives ADF and ADL fiber fractions, content of P, Na and K, most of the amino acid levels and on nutritional values of the protein measured by means of CS and EAAI indices.


2016 ◽  
Vol 5 (1) ◽  
pp. 97 ◽  
Author(s):  
B. I. Balogun ◽  
S. J. Oniye ◽  
J. Auta ◽  
C. A. M. Lakpini ◽  
F. O. Abeke

This study was conducted to evaluate the suitability of soaked <em>Bauhinia monandra</em> (Kutz) seed as alternative protein source for <em>Clarias gariepinus</em> and to determine cost of feed compounded. The proximate analysis of the diet (crude protein, crude fibre, ash, moisture content and lipid) were determined using AOAC (1980). Data for each parameter was subjected to one way analysis of variance (ANOVA) while means of various results were compared at 5% level of significance. A preliminary study was conducted to determine the best processing method that reduced anti-nutrients to the minimum level without impairing nutrients composition. Boiled, toasted and soaked seeds were used. <em>Bauhinia</em> seeds soaked for 96 hours had least concentration of anti-nutrients. The experimental diets was formulated to contain soaked <em>Bauhinia</em> seed meal (SBSM) at levels of 25%, 50% and 75% inclusion (Diets 2, 3 and 4 respectively)  with two diets acting as control (Diets 1 and 5). All diets were isonitrogenous (40% crude protein) and isocaloric (3212kcal/kg). A 12 weeks feeding trial was conducted using juveniles which were randomly distributed into 12 improvised non-recirculatory and semi-flow through indoor plastic tanks (52cm X 34cm X 33.5cm) at a stocking rate of 10 fish per tank and three (3) replicates per treatment. The experimental design was complete randomized. The fish were fed at 5% body weight, twice daily. Diets with higher inclusion levels of SBSM (diets 3 and 4) significantly depressed growth performance of fish. The variations observed in the Specific Growth Rate (SGR), Feed Conversion Ratio (FCR), Feed Efficiency Ratio (FER) and Protein Efficiency Ratio (PER) were associated with the anti-nutrients present in the diets, these parameters reduced with increasing levels of SBSM in the diets of fish. Based on the relative cost of diets per unit weight gain and protein gain, diet 2 (25% SBSM) was most economical. The results suggested that SBSM can be used to substitute up to 25% levels of dietary protein in <em>C. gariepinus</em> juveniles without significant reduction in growth.


2007 ◽  
Vol 13 (2) ◽  
pp. 165-171 ◽  
Author(s):  
C.A. Ezeokonkwo

The effects of dry- (roasting) and moist-heat (boiling) treatments on the biochemical characteristics of Terminalia catappa (tropical almond) seed were determined. There were no significant differences in crude protein, fat, ash, and carbohydrate content of the seeds with respect to processing. Boiling increased significantly ( P < 0.05) the crude fibre, however roasting reduced it. Roasting had a better effect on the total dietary fibre (TDF) and gross energy level of the seeds than boiling. There were no significant differences in the effects of both processes on the amino acid, fatty acid and sugar profiles of the seeds. Roasting improved the potassium, phosphorus, copper and iodine, reducing the magnesium, calcium, zinc and iron contents. Boiling improved the sodium and selenium and reduced the potassium, magnesium and calcium levels.


1950 ◽  
Vol 7d (10) ◽  
pp. 563-566 ◽  
Author(s):  
Phyllis W. Ney ◽  
Catherine P. Deas ◽  
H. L. A. Tarr

The essential amino acids arginine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, valine, tryptophane and tyrosine were determined in the following fishery products using microbiological assay technique: fish meals, stickwaters (fish solubles), condensed fish solubles, liver, commercial liver hydrolysate, frozen pink salmon viscera, chum salmon fingerlings and herring scales.


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