Different forms and frequencies of soybean oil supplementation do not alter rumen fermentation in grazing heifers

2017 ◽  
Vol 57 (3) ◽  
pp. 530
Author(s):  
M. C. A. Santana ◽  
G. Fiorentini ◽  
J. D. Messana ◽  
P. H. M. Dian ◽  
R. C. Canesin ◽  
...  

Over the productive cycle, the performance of cattle in grazing systems may be compromised, and feed supplementation is a strategy to establish nutritional balance and maximise the performance of grazing animals. The objective of this study was to evaluate the effect of different supplementation frequencies (i.e. daily supplementation (DS) versus Monday, Wednesday and Friday (MWF)) with different forms of soybean oil (i.e. soybean grain (SG), soybean oil (SO) and rumen-protected fat (RPF)) on the nutrient digestion and rumen fermentation of heifers grazing Brachiaria brizantha cv. Marandu pastures. Twelve crossbred heifers (1/4 Nellore × 1/4 Santa Gertrudis × 1/2 Braunvieh) with an initial bodyweight of 236.3 ± 27.6 kg were distributed in a completely randomised design with a factorial arrangement 3 × 2 (three diets and two supplementation frequencies). There was no interaction among the SO form or supplementation frequency on dry matter intake and dry matter digestibility (P > 0.05). The animals supplemented with SG exhibited lower nitrogen intake and absorption compared with animals that were fed SO (P < 0.05). The efficiency of microbial synthesis was not influenced by the diet or supplementation frequency (P > 0.05). Forms of SO affected the rumen pH; animals fed SO showed pH values similar to those of animals receiving SG but lower values than animals fed RPF (P < 0.05). Animals supplemented with DS exhibited higher levels of rumen ammonia nitrogen relative to those supplemented MWF. The reduction of supplementation frequency (from DS to MWF k) in our experimental conditions can be used to increase the efficiency of pasture utilisation without effects in fermentation activity of the rumen microbial population. Supplementation with SO is a strategy to increase propionic acid concentration and reduce A : P ratio, which is an indicator of improvement of the efficiency of the ruminal energy utilisation.


2010 ◽  
Vol 90 (3) ◽  
pp. 413-420 ◽  
Author(s):  
L R Tager ◽  
K M Krause

A 12-unit continuous culture system was used in a complete randomized design to study the effects of no oil (CON), cinnamaldehyde oil (CIN), eugenol oil (EUG), and capsicum oil (CAP) (500 mg L-1 d -1) with a 45:55 forage:concentrate ratio (dry matter basis) ration on rumen fermentation. Dry matter digestibility did not differ among treatments. Organic matter digestibility tended to decrease with CIN. Digestibility of neutral detergent and acid detergent fiber tended to be highest with CAP. Crude protein digestibility and bacterial nitrogen flow was depressed with CIN and EUG. CIN tended to decrease microbial protein synthesis and increase effluent ammonia nitrogen. Total volatile fatty acid production did not differ among treatments; however, isovalerate production tended to be highest with CAP. CIN and EUG had higher mean pH, spent fewer hours per day and had smaller area under the curve at pH < 5.6 and 5.8. CAP had smaller area under the curve at pH < 5.6. Supplementation with these oils at the current dose had limited effects on rumen fermentation, with the majority of effects observed being mainly attributable to the very high dosage of oil used. Key words: Dairy cow, essential oil, continuous culture, rumen fermentation, rumen pH



2017 ◽  
Vol 48 (2) ◽  
pp. 63-69
Author(s):  
M. Joch ◽  
V. Kudrna ◽  
B. Hučko

AbstractThe objective of this study was to determine the effects of geraniol and camphene at three dosages (300, 600, and 900 mg l-1) on rumen microbial fermentation and methane emission in in vitro batch culture of rumen fluid supplied with a 60 : 40 forage : concentrate substrate (16.2% crude protein, 33.1% neutral detergent fibre). The ionophore antibiotic monensin (8 mg/l) was used as positive control. Compared to control, geraniol significantly (P < 0.05) reduced methane production with increasing doses, with reductions by 10.2, 66.9, and 97.9%. However, total volatile fatty acids (VFA) production and in vitro dry matter digestibility were also reduced (P < 0.05) by all doses of geraniol. Camphene demonstrated weak and unpromising effects on rumen fermentation. Camphene did not decrease (P > 0.05) methane production and slightly decreased (P < 0.05) VFA production. Due to the strong antimethanogenic effect of geraniol a careful selection of dose and combination with other antimethanogenic compounds may be effective in mitigating methane emission from ruminants. However, if a reduction in total VFA production and dry matter digestibility persisted in vivo, geraniol would have a negative effect on animal productivity.



2015 ◽  
Vol 153 (6) ◽  
pp. 1135-1145 ◽  
Author(s):  
J. W. ZHOU ◽  
J. D. MI ◽  
A. A. DEGEN ◽  
X. S. GUO ◽  
H. C. WANG ◽  
...  

SUMMARYA comparative study of the effect of dietary nitrogen (N) content [Low: 11·0; Medium-Low (MLow): 16·7; Medium-High (Mhigh): 23·1; High: 29·2 N g/kg dry matter (DM)] on apparent digestibilities, rumen fermentation and N balance was conducted in coarse wool Tibetan sheep and Gansu Alpine fine-wool sheep at Wushaoling in the northeast of the Qinghai-Tibetan Plateau. It was hypothesized that responses would differ between breeds and that responses would favour Tibetan over fine-wool sheep at low N intakes. Eight wethers [four Tibetan sheep and four fine-wool sheep, 20–24 months old; body weight ± standard deviation was 52 ± 3·2 kg] were used in two concurrent 4 × 4 Latin square designs. Dry matter, organic matter, neutral detergent fibre and acid detergent fibre digestibilities were higher in Tibetan than fine-wool sheep when fed the Low, MLow and High N diets while N retention was higher when the animals were fed the Low and MLow N diets. Tibetan sheep had a higher rumen pH than fine-wool sheep; however, total volatile fatty acids were similar between breeds. Molar proportions of acetate were higher but propionate and butyrate lower in Tibetan than fine-wool sheep. In addition, Tibetan sheep had higher concentrations of ruminal free amino acid-N and soluble protein-N than fine-wool sheep. Plasma and saliva urea-N concentrations were higher in Tibetan than fine-wool sheep when supplied with the Low N diet. It was concluded that Tibetan sheep were better able to cope with low N feed than fine-wool sheep because of the higher N retention and higher DM and fibre digestibilities with Low and MLow diets.



1983 ◽  
Vol 23 (120) ◽  
pp. 14 ◽  
Author(s):  
NG Yates ◽  
RJ Moir

Oaten hay in either long or milled form, with or without a continuous intra-ruminal infusion of urea at the rate of 160 g/d, was offered ad libitum to four rumen fistulated steers in a 4 x 4 Latin square design experiment. Milling increased dry matter intake from 49.2 to 65.4 g/kg LW0 75.d (P< 0.001) and decreased mean retention time from 66.7 to 54.2 h (P< 0.001). Rates of cellulose digestion and dry matter digestibilities were similar for long and milled hay. Urea increased dry matter intake (from 49.0 to 65.6 g/kg LW0 75.d; P< 0.001), dry matter digestibility (from 58.1 to 65.3%; P< 0.01) cellulose digestibility (from 48.5 to 63.1%; P< 0.01) and the rate of cellulose digestion (P< 0.001), and decreased mean retention time from 65.3 to 55.6 h ( P < 0.01). Digestible dry matter intakes increased 29% with milling and 52% with urea and the effects were additive. Urea increased digestible cellulose intake to a greater extent with milled hay (91 %) than with long hay (64%). Digestible dry matter intake was closely related to liveweight change. When urea was given liveweight gain was higher for milled hay than for long hay (0.69 vs 0.11 kg/head.d) and without urea liveweight loss was less for milled hay than for long hay (-0.07 vs -0.45 kg/head.d). Nitrogen digestibility and balance were significantly higher when urea was given. Total volatile fatty acid concentrations were significantly higher and rumen pH significantly lower with urea compared with no urea and with milled hay compared with long hay. Urea increased rumen-ammonia concentration from 1.65 to 19.68 mg1100 ml (P< 0.001) and total free bacterial concentration (P < 0.05). The results demonstrate significant and additive benefits from the administration of urea to hay containing 0.8% nitrogen and from milling through a screen size of 7.6 mm.



1993 ◽  
Vol 56 (1) ◽  
pp. 85-92 ◽  
Author(s):  
D. Cardenas Garcia ◽  
C. J. Newbold ◽  
H. Galbraith ◽  
J. H. Topps ◽  
X. B. Chen ◽  
...  

AbstractThe effect of including 14 g urea with either 75 g dry matter (DM) from sugar cane molasses (UM) or Colombian rice polishings (RP) at three levels, 68 (RP1), 137 (RP2) or 203 (RP3) g DM on grass hay DM intake and on rumen fermentation was investigated. An incomplete Latin-square design was used and each experimental period was divided into 12 days for adaptation to each diet followed by 9 days in metabolism cages when all measurements were made using five adult sheep.Pooled mean values for rumen metabolites (five samples per day) were calculated. Rumen pH was not affected by the nature of the supplements. Concentrations of volatile fatty acids (VFA) (UM 92·8, RP1 84·2, RP2 86·4, and RP3 84·0 (s.e.d. 3·4) mmol/l) and lactate (UM 2·0, RP1 1/6, RP2 1/7, RP3 1/8 (s.e.d. 0·014) mmol/l) (UM v. RP, P < 0·05 and P < 0·10 respectively) were lower when RP were given, while concentration of branched and longer chain VFA (26·5, 34·0, 31·1 and 33·5 (s.e.d. 1·6) mmol/mol total VFA, UM v. RP, P < 0·01) and ammonia (98, 131, 141, 137 (s.e.d. 16·1) mg/l, UM v. RP, P < 0·05) were increased. Numbers of rumen protozoa (1·6, 3·2, 2·7, 3·3 (s.e.d. 0·75) × 105 per ml, UM v. RP, P < 0·20) tended to be higher 2 h after feeding when RP rather than UM were given. However, hay DM intake (1050, 960, 960, 880 (s.e.d. 45·3) g/day, UM v. RP, P < 0·05), and microbial protein supply (11·7, 9·3,11·1,10·8 (s.e.d. 0·59) g N per day, UM v. RP, P < 0·05), estimated from urinary purine excretion were reduced by feeding RP instead of UM.At the levels of inclusion tested RP did not increase the efficiency of rumen fermentation and were not as effective a supplement with urea as was molasses for a low-quality forage diet.



2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 424-424
Author(s):  
Amanda P Schiff ◽  
Kyle McLeod ◽  
James L Klotz ◽  
Vaughn Holder ◽  
David L Harmon

Abstract Programmed Nutrition Beef Program (Alltech Inc.) is a supplement that could reduce the use of feed additives such as Monensin and Tylosin in feedlots. The objective of this study was to examine changes in rumen fermentation when feeding Monensin/Tylosin and Programmed Nutrition. Eight steers (BW = 363 ± 22 kg) were used in a replicated 4 x 4 Latin square design experiment in a 2 x 2 factorial treatment structure where animals were fed a high-concentrate diet at 2.0 x NEm. Treatments were Control (C; conventional trace mineral supplement), Control + Monensin + Tylosin (MT), Programmed Nutrition (PN; Programmed Nutrition Beef Finisher), or Programmed Nutrition + Monensin + Tylosin (PNMT), incorporated daily to the diet at 75 g/day. Rumen pH was measured continuously for 48 h, rumen fluid was collected every 2 h for 48 h and analyzed for VFA. Dietary digestibility, nitrogen and energy balance were determined by collection of total urine and fecal output for 7 days, and indirect calorimetry for 48 h. Protein turnover was determined via 15N-glycine end-product method. The experiment was analyzed as a replicated Latin square design with mixed models of SAS (SAS Inst. Inc.). Mean differences were analyzed using LSD, rumen VFA and pH data were analyzed using repeated measures. There were no differences between treatments in DMI, ADG, dry matter digestibility and rumen pH. Addition of MT to the diet lowered fecal N output (P = 0.0092), ruminal valerate concentration (P = 0.0125) and molar proportion (P = 0.0235). When PN was fed in combination with MT it decreased ADF digestibility (P = 0.0342), protein turnover (P = 0.0211), protein synthesis (P = 0.0362) and protein degradation (P = 0.0292). Although no differences in ADG and dry matter digestibility were observed, significant metabolic changes occurred when supplementing PN with or without MT.



2018 ◽  
Vol 18 (4) ◽  
pp. 857-877 ◽  
Author(s):  
Khouloud Khachlouf ◽  
Houda Hamed ◽  
Radhouane Gdoura ◽  
Ahmed Gargouri

AbstractIn recent years, the use of both natural and synthetic zeolites in livestock feeds fed to lactating cows has increased, mainly to improve their performance, health, and to protect against mycotoxins intoxication. Data of scientific literature were compiled to analyze the effects of the incorporation of non-nutritional adsorbent zeolite on production performance and ruminal environment parameters of lactating cows. At moderate levels (200–400 g/cow/day), milk yield was increased by zeolite. Milk fat and protein contents and DMI were not altered and all ruminal parameters were improved: acetate was enhanced, propionate was reduced and consequently, acetate to propionate ratio was increased. The rumen pH was increased and rumen ammonia nitrogen was reduced. When the level of zeolite exceeded 400 g/d/cow, all production and ruminal parameters were negatively altered. These data suggest that zeolite level in the diet has a significant effect on the response of dairy production and ruminal environment characteristics.



2020 ◽  
Vol 41 (1) ◽  
pp. 237
Author(s):  
João Pedro Velho ◽  
Pagiel Bernardi Zardin ◽  
Clóves Cabreira Jobim ◽  
Ione Maria Pereira Haygert-Velho ◽  
Dileta Regina Moro Alessio ◽  
...  

The objective of this study was to conduct a meta-analysis of scientific publications from January 1994 to December 2014, to evaluate the chemical composition of corn plants, green forage, and silages of three different types of maize hybrids (simple, double, and triple) used in experimental conditions in Brazil. The sub-bases Corn Cultivation and Green Fodder were composed of 49 experiments, consisting of 481 treatments, and the sub-base Silage consisted of 24 studies composed of 163 treatments, involving 478 silos. In the Corn Cultivation sub-base, the variables of spacing, plant height, grain yield, organic matter content, and total carbohydrates of maize silage differed (P < 0.05) according to the hybrid type. The number of corn plants per hectare, number of spikes per plant, and the levels of neutral detergent fiber, acid detergent fiber, hemicellulose, and lignin did not differ (P > 0.05) according to the hybrid type. In the Green Forage sub-base (ensilage), the main difference (P < 0.05) was regarding the in vitro dry matter digestibility, whereby the triple hybrids (59.38%) had lower values than the simple (73.05%) and double (72.74%) hybrids. In the Silage sub-base, the variables of organic matter, total carbohydrates, cellulose, in vitro dry matter digestibility, and pH differed (P < 0.05) according to the hybrid type. While the variables dry matter, non-fibrous carbohydrates, neutral detergent fiber, acid detergent fiber, hemicellulose, lignin, total digestible nutrients, ethereal extract, crude protein, and ammonium nitrogen did not differ (P > 0.05) according to the hybrid type. The variability in the determination of parameters, such as dry matter yield, dry matter content, and non-fibrous carbohydrates of corn silage, and the non-determination of the starch content demonstrated that the chemical compositions of Brazilian corn silage have not yet been fully clarified. The types of maize hybrids (simple, double, and/or triple) mainly differ in terms of grain production. In many situations, the silage processes are not conducted in an ideal way, and the differences as a function of the maize plants (type of hybrid) are minimized due to losses during the ensiling process.



1963 ◽  
Vol 43 (2) ◽  
pp. 264-270 ◽  
Author(s):  
J. G. Manns ◽  
J. P. Bowland

Rats and pigs received diets containing solvent-extracted rapeseed oil meal (R.O.M.) substituted on an equivalent protein basis for 0 to 100 per cent of the dietary soybean oil meal (S.O.M.). R.O.M. represented up to 15.6 per cent of the total diet. Apparent nitrogen (N), energy and dry matter (D.M.) digestibility coefficients were reduced during growth (4 to 5 weeks of age) when rats received R.O.M. in replacement for 50 or 100 per cent of the dietary S.O.M., and during gestation when they received R.O.M. in replacement for 25 to 100 per cent of the S.O.M. Results with pigs were similar but less consistent. Retention of gross N or digested N was lower in rats receiving R.O.M. in substitution for over 50 per cent of the S.O.M. during growth or over 25 per cent of the S.O.M. during gestation. A supplement of 0.2 per cent L-lysine added to the diet containing the highest level of R.O.M. had no influence on digestibility or retention in rats but improved N, energy and D.M. digestibilities for pigs at 75 pounds liveweight and during gestation and lactation. Sex of rats did not influence digestibility coefficients, but boars were more efficient than gilts in digesting energy and D.M.



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