Matching physiological traits and ion concentrations associated with salt stress in cowpea genotypes

2002 ◽  
Vol 53 (11) ◽  
pp. 1243 ◽  
Author(s):  
Bernardo Murillo-Amadot ◽  
Enrique Troyo-Diéguez ◽  
Raúl López-Aguilar ◽  
Alejandro López-Cortés ◽  
Clara L. Tinoco-Ojanguri ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Salinity Tolerance ◽  
Growth Stage ◽  
Seedling Survival ◽  
Nutrient Deficiency ◽  
Chloride Concentration ◽  
Sodium Concentration ◽  
Ion Concentrations ◽  
Survival Percentage

The salt tolerance of 25 cowpea genotypes (Vigna unguiculata L. Walp.) was studied during early vegetative growth. Salinity treatments were applied by irrigating with a nutrient solution containing 0, 85, and 170 mmol NaCl/L. Seedling survival decreased linearly as salinity increased, but this enabled cowpea genotypes to be ranked for salinity tolerance according to the magnitudes of slopes of regression of survival percentage on salinity. Sodium concentration was higher in roots than in shoots in all genotypes, and increased significantly in both roots and shoots as salinity increased. Chloride concentration in both roots and shoots increased with increasing salinity in all genotypes, and was higher in shoots than in roots at 85 and 170 mmol NaCl/L. In some cases, Ca, Mg, K, and P concentrations were reduced by an increase in salinity, but none of the genotypes appeared to suffer any nutrient deficiency. We observed wide differences in responses to salinity, and our results suggest that during the growth stage studied, 7 of the 25 genotppes tested could be classified as tolerant or relatively tolerant to salinity (Sonorense, CB3, CB27, Cuarenteño, CB46, Paceño, and IT82D-889).

scholarly journals Physiological and Biochemical Responses to Salt Stress of Alfalfa Populations Selected for Salinity Tolerance and Grown in Symbiosis with Salt-Tolerant Rhizobium

Agronomy ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 569
Author(s):  
Annick Bertrand ◽  
Craig Gatzke ◽  
Marie Bipfubusa ◽  
Vicky Lévesque ◽  
Francois P. Chalifour ◽  
...  
Keyword(s):  
Amino Acids ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Water Content ◽  
Salinity Tolerance ◽  
Osmotic Adjustment ◽  
Recurrent Selection ◽  
Aromatic Amino Acids ◽  
Nacl Stress ◽  
Salt Tolerant

Alfalfa and its rhizobial symbiont are sensitive to salinity. We compared the physiological responses of alfalfa populations inoculated with a salt-tolerant rhizobium strain, exposed to five NaCl concentrations (0, 20, 40, 80, or 160 mM NaCl). Two initial cultivars, Halo (H-TS0) and Bridgeview (B-TS0), and two populations obtained after three cycles of recurrent selection for salt tolerance (H-TS3 and B-TS3) were compared. Biomass, relative water content, carbohydrates, and amino acids concentrations in leaves and nodules were measured. The higher yield of TS3-populations than initial cultivars under salt stress showed the effectiveness of our selection method to improve salinity tolerance. Higher relative root water content in TS3 populations suggests that root osmotic adjustment is one of the mechanisms of salt tolerance. Higher concentrations of sucrose, pinitol, and amino acid in leaves and nodules under salt stress contributed to the osmotic adjustment in alfalfa. Cultivars differed in their response to recurrent selection: under a 160 mM NaCl-stress, aromatic amino acids and branched-chain amino acids (BCAAs) increased in nodules of B-ST3 as compared with B-TS0, while these accumulations were not observed in H-TS3. BCAAs are known to control bacteroid development and their accumulation under severe stress could have contributed to the high nodulation of B-TS3.

scholarly journals QTLs for seedling traits under salinity stress in hexaploid wheat

2018 ◽  
Vol 48 (3) ◽  
Author(s):  
Yongzhe Ren ◽  
Yanhua Xu ◽  
Wan Teng ◽  
Bin Li ◽  
Tongbao Lin
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Salinity Tolerance ◽  
Hexaploid Wheat ◽  
Dry Weight ◽  
Interval Mapping ◽  
Mapping Method ◽  
Stress Conditions ◽  
Wheat Varieties ◽  
Seedling Traits

ABSTRACT: Soil salinity limits agricultural production and is a major obstacle for increasing crop yield. Common wheat is one of the most important crops with allohexaploid characteristic and a highly complex genome. QTL mapping is a useful way to identify genes for quantitative traits such as salinity tolerance in hexaploid wheat. In the present study, a hydroponic trial was carried out to identify quantitative trait loci (QTLs) associated with salinity tolerance of wheat under 150mM NaCl concentration using a recombinant inbred line population (Xiaoyan 54×Jing 411). Values of wheat seedling traits including maximum root length (MRL), root dry weight (RDW), shoot dry weight (SDW), total dry weight (TDW) and the ratio of TDW of wheat plants between salt stress and control (TDWR) were evaluated or calculated. A total of 19QTLs for five traits were detected through composite interval mapping method by using QTL Cartographer version 2.5 under normal and salt stress conditions. These QTLs distributed on 12 chromosomes explained the percentage of phenotypic variation by individual QTL varying from 7.9% to 19.0%. Among them, 11 and six QTLs were detected under normal and salt stress conditions, respectively and two QTLs were detected for TDWR. Some salt tolerance related loci may be pleiotropic. Chromosome 1A, 3A and 7A may harbor crucial candidate genes associated with wheat salt tolerance. Our results would be helpful for the marker assisted selection to breed wheat varieties with improved salt tolerance.

scholarly journals Genomic and transcriptomic analysis reveal molecular basis of salinity tolerance in a novel strong salt-tolerant rice landrace Changmaogu

Rice ◽  
2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Bing-Rui Sun ◽  
Chong-Yun Fu ◽  
Zhi-Lan Fan ◽  
Yu Chen ◽  
Wen-Feng Chen ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Salinity Tolerance ◽  
Molecular Basis ◽  
Carotenoid Biosynthesis ◽  
Seedling Stage ◽  
Differentially Expressed ◽  
Salt Tolerant ◽  
Transcriptomic Sequencing ◽  
Rice Landrace

Abstract Background Salt stress is an important factor that limits rice yield. We identified a novel, strongly salt tolerant rice landrace called Changmaogu (CMG) collected from a coastal beach of Zhanjiang, Guangdong Province, China. The salt tolerance of CMG was much better than that of the international recognized salt tolerant rice cultivar Pokkali in the germination and seedling stages. Results To understand the molecular basis of salt tolerance in CMG, we performed BSA-seq for two extreme bulks derived from the cross between CMG and a cultivar sensitive to salt, Zhefu802. Transcriptomic sequencing was conducted for CMG at the germination and young seedling stages. Six candidate regions for salt tolerance were mapped on Chromosome 1 by BSA-seq using the extreme populations. Based on the polymorphisms identified between both parents, we detected 32 genes containing nonsynonymous coding single nucleotide polymorphisms (SNPs) and frameshift mutations in the open reading frame (ORF) regions. With transcriptomic sequencing, we detected a large number of differentially expressed genes (DEGs) at the germination and seedling stages under salt stress. KEGG analysis indicated two of 69 DEGs shared at the germination and seedling stages were significantly enriched in the pathway of carotenoid biosynthesis. Of the 169 overlapping DEGs among three sample points at the seedling stage, 13 and six DEGs were clustered into the pathways of ABA signal transduction and carotenoid biosynthesis, respectively. Of the 32 genes carrying sequence variation, only OsPP2C8 (Os01g0656200) was differentially expressed in the young seedling stage under salt stress and also showed sequence polymorphism in the ORFs between CMG and Zhefu802. Conclusion OsPP2C8 was identified as the target candidate gene for salinity tolerance in the seedling stage. This provides an important genetic resource for the breeding of novel salt tolerant rice cultivars.

scholarly journals AtCIPK16 Mediates Salt Stress Through Phytohormones and Transcription Factors

2020 ◽  
Author(s):  
Shanika L. Amarasinghe ◽  
Wenmian Huang ◽  
Nathan S. Watson-Haigh ◽  
Matthew Gilliham ◽  
Stuart J. Roy ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factor ◽  
Transcription Factors ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Salinity Tolerance ◽  
Food Production ◽  
Rna Seq ◽  
Productivity Losses ◽  
Global Food

AbstractSoil salinity causes large productivity losses for agriculture worldwide. “Next-generation crops” that can tolerate salt stress are required for the sustainability of global food production. Previous research in Arabidopsis thaliana aimed at uncovering novel factors underpinning improved plant salinity tolerance identified the protein kinase AtCIPK16. Overexpression of AtCIPK16 enhanced shoot Na+ exclusion and increased biomass in both Arabidopsis and barley. Here, a comparative transcriptomic study on Arabidopsis lines expressing AtCIPK16 was conducted in the presence and absence of salt stress, using an RNA-Seq approach, complemented by AtCIPK16 interaction and localisation studies. We are now able to provide evidence for AtCIPK16 activity in the nucleus. Moreover, the results manifest the involvement of a transcription factor, AtTZF1, phytohormones and the ability to quickly reach homeostasis as components important for improving salinity tolerance in transgenics overexpressing AtCIPK16. Furthermore, we suggest the possibility of both biotic and abiotic tolerance through AtCIPK16, and propose a model for the salt tolerance pathway elicited through AtCIPK16.

Reliable and easy screening technique for salt tolerance of citrus rootstocks under controlled environments

2002 ◽  
Vol 53 (6) ◽  
pp. 653 ◽  
Author(s):  
M. R. García ◽  
G. P. Bernet ◽  
J. Puchades ◽  
I. Gómez ◽  
E. A. Carbonell ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Seedling Survival ◽  
Hydroponic Culture ◽  
Breeding Programs ◽  
Screening Technique ◽  
Low Salinity ◽  
Survival Percentage ◽  
Volkamer Lemon ◽  
Cleopatra Mandarin

Three salt tolerance experiments using 5 common citrus rootstocks were carried out to find a reliable and easy screening technique for salt tolerance in breeding programs. The experiments were: (1) in vitro seed culture where salt tolerance was mainly evaluated as germination percentage, (2) hydroponic culture of 2-month-old seedlings where salt tolerance was mainly evaluated as survival percentage, and (3) hydroponic culture of satsuma-rootstock combinations where salt tolerance was evaluated by leaf and fruit characters. Treatments were: 4 mm K2CO3 and 0-100 mm NaCl in Expt 1; 3.5 mm K2CO3 and 0-50 mm NaCl, with and without K2CO3, in Expt�2; and 25 mm NaCl in Expt 3. Volkamer lemon was the most salt-sensitive genotype during seed germination (Expt�1), whereas Troyer citrange was the most sensitive when used as rootstock of satsuma (Expt 3). For seedling survival (Expt 2), the trifoliate orange variety Flying dragon showed the highest survival percentage, and chloride content of satsuma leaves and fruit juice were high on this rootstock under salinity (Expt 3). Alkalinity (pH = 8.5) greatly affected seedling survival of Cleopatra mandarin and Volkamer lemon (Expt 2), probably due to major disturbances in seedling nutrition. Analysis of trait values for the rootstocks in the different saline treatments in both the in vitro germination and the seedling survival experiments revealed some significant changes compared with control conditions. Most of these changes were not consistent between genotypes, except for chloride concentration in both shoot ([Cl]s) and root ([Cl]r). The ordering of genotypes for salt tolerance found in the literature, which corresponds to the ordering as chloride excluders in our satsuma Expt 3, agrees with the inverse ordering of genotypes regarding the increment of both [Cl]s and the ratio [Cl]s/[Cl]r from control to low salinity, but does not agree with salt tolerance measured as a percentage of germination or seedling survival. The increments of both [Cl]s and the ratio [Cl]s/[Cl]r from control to low salinity are suggested as criteria for early selection of salt-tolerant citrus rootstocks. Three salt tolerance mechanisms have been observed: chloride exclusion, water saving, and accumulation of soluble solids. They all seem to be presented by Cleopatra mandarin when used as rootstock, supporting its utilisation as donor of salt tolerance in breeding programs of citrus rootstocks.

scholarly journals Insights into the Physiological and Biochemical Impacts of Salt Stress on Plant Growth and Development

Agronomy ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 938 ◽  
Author(s):  
Muhammad Adnan Shahid ◽  
Ali Sarkhosh ◽  
Naeem Khan ◽  
Rashad Mukhtar Balal ◽  
Shahid Ali ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Salinity Tolerance ◽  
Antioxidant Activities ◽  
Soil Salinization ◽  
Crop Plants ◽  
Cellular Damage ◽  
Relative Water ◽  
Ionic Imbalance ◽  
Highly Correlated

Climate change is causing soil salinization, resulting in crop losses throughout the world. The ability of plants to tolerate salt stress is determined by multiple biochemical and molecular pathways. Here we discuss physiological, biochemical, and cellular modulations in plants in response to salt stress. Knowledge of these modulations can assist in assessing salt tolerance potential and the mechanisms underlying salinity tolerance in plants. Salinity-induced cellular damage is highly correlated with generation of reactive oxygen species, ionic imbalance, osmotic damage, and reduced relative water content. Accelerated antioxidant activities and osmotic adjustment by the formation of organic and inorganic osmolytes are significant and effective salinity tolerance mechanisms for crop plants. In addition, polyamines improve salt tolerance by regulating various physiological mechanisms, including rhizogenesis, somatic embryogenesis, maintenance of cell pH, and ionic homeostasis. This research project focuses on three strategies to augment salinity tolerance capacity in agricultural crops: salinity-induced alterations in signaling pathways; signaling of phytohormones, ion channels, and biosensors; and expression of ion transporter genes in crop plants (especially in comparison to halophytes).

scholarly journals Tolerance of castor bean cultivars under salt stress

2016 ◽  
Vol 20 (6) ◽  
pp. 557-563 ◽  
Author(s):  
Francisco V. da S. Sá ◽  
Emanoela P. de Paiva ◽  
Evandro F. de Mesquita ◽  
Antonio M. P. Bertino ◽  
Marcelo A. Barbosa ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Castor Bean ◽  
Dry Matter ◽  
Growth Stage ◽  
Dry Matter Accumulation ◽  
Initial Growth ◽  
Initial Growth Stage ◽  
Salinity Levels

ABSTRACT This study aimed to evaluate the tolerance of castor bean cultivars under salt stress during the initial growth stage. The experiment was set in randomized blocks, in a 5 x 4 factorial scheme, resulting in 20 treatments, 5 salinity levels (0.6-control, 1.2, 1.8, 2.4 and 3.0 dS m-1) and 4 castor bean cultivars (BRS Energy, LA Guarani, BRS Gabriela and IAC 028)] with 3 replicates. At 30 days after sowing, plants were evaluated for growth, dry matter accumulation and salt tolerance. Increased salinity levels in the water used for irrigation reduced the growth of all the studied cultivars. The salt tolerance of the studied castor bean cultivars follows the following order: BRS Energia > BRS Gabriela = IAC 028 > LA Guarani.

scholarly journals Salt Tolerant Gossypium hirsutum L. Cultivars are Mostly Long-Staple Length Cultivars

2022 ◽  
Author(s):  
Rachel Predeepa ◽  
Ranjith Kumar ◽  
George C. Abraham ◽  
T. S. Subramanian
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Gossypium Hirsutum ◽  
Salinity Tolerance ◽  
Molecular Techniques ◽  
Salt Tolerant ◽  
Cotton Lint ◽  
Cropping Season ◽  
Cotton Plants ◽  
Staple Length

Abstract Background: Cotton is a major cash crop in the global and, in particular, the Indian markets, playing an important economic role in the textile and oil industries. The cotton plant is one of the highly bred plants that is highly sensitive to salt stress. As cotton is a non-food crop, the availability of non-saline terrain and water for the cultivation of cotton plants is only next to other food crops, thereby posing a need to better understand the salt tolerance of this plant. Gossypium hirsutum L. cultivars MCU 5, LRA 5166, and SVPR 2 were selected based on exomorphic traits like staple length and cropping season so that the genotypic responses to salt stress and salt shock can be compared for interpreting the effects of salinity on in vitro germination. Thus, this study aims to establish genotypic dependence on salinity tolerance. Results: The results affirmed genotypic variation in salinity tolerance, with MCU 5 tolerating salt stress better than LRA 5166 and SVPR 2 in all the observed stages of growth of the plant and the parameters measured. Further salt-tolerant cotton varieties were observed to be long-staple length varieties; staple length is the fiber character of the cotton lint. Moreover, salt tolerance in the vegetative growth stage of cotton plants is not independent of the germination stage of the plant.Conclusion: Nevertheless, the correlation of genotypic dependence to morphological characteristics, in particular, staple length (and cropping season), is of agronomic and commercial significance. Further research by screening and investigating a greater number of cultivars using biochemical and molecular techniques will provide a better understanding of this observed phenotypical relationship to the genotypes of cotton cultivars under salt stress.

scholarly journals Improvement in Salinity Tolerance in Rice Using Biotechnology

2021 ◽  
Vol 9 (11) ◽  
pp. 551-560
Author(s):  
Ankita Roy
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Salinity Tolerance ◽  
Rice Crop ◽  
Cereal Crop ◽  
Rice Varieties ◽  
The Past ◽  
The Future ◽  
Staple Diet ◽  
Held Back

Abstract: Rice has been amongst the most important cereal crop on earth, or more correctly the second most staple diet of people after wheat. The statement is self-explanatory to the demand of rice worldwide and the supply. But with increasing population, the demand of rice is also undoubtedly increasing. The increase in supply is though held back by many factors that adversely affect the growth of a healthy rice crop. One of these factors is salinity. Rice being a glycophyte has very low tolerance to salt, and salt stress results in a damaged yield. However, rice has its own mechanisms for salt tolerance to a certain level, the knowledge of which scientists have put in use to develop the few improvements in rice varieties providing them with better tolerance. Though these improvements have faced many challenges themselves, science still strives to make further achievements. This study aims to highlight the mechanisms rice use to tolerate salt up to a certain level and how they are affected by salinity at levels above that. It also aims to enumerate the methods biotechnology has developed in the past years to improve the varieties, the advantages they have had, the increase in yield they have shown and the challenges they have had to face. Also, it includes if there are any further developments undergoing and the future scope of salinity tolerance increase in rice, with the help of biotechnology.

Alfalfa (Medicago sativa L.) clones tolerant to salt stress: in vitro selection

2013 ◽  
Vol 8 (8) ◽  
pp. 765-776 ◽  
Author(s):  
Angela Campanelli ◽  
Claudia Ruta ◽  
Irene Morone-Fortunato ◽  
Giuseppe Mastro
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Medicago Sativa ◽  
In Vitro Selection ◽  
Growth Parameters ◽  
Sodium Content ◽  
Saline Stress ◽  
Survival Percentage ◽  
Medicago Sativa L

AbstractIn order to quickly and efficiently evaluate the salt tolerance of alfalfa, salinity tests were conducted on Medicago sativa L. var. australis, var. icon, var. loi, and var. gea, under in vitro conditions. Pregerminated seeds of four varieties were subjected to five different NaCl concentrations (0, 50, 100, 150, 200 mM). The influence of saline stress was estimated on the basis of survival percentage, growth parameters, and electrolyte leakage. The seedlings surviving on the medium enriched with salt at the highest concentration were presumed to be tolerant and represented the mother plants for the production of in vitro clones. In the following step, the clones were evaluated in vitro to confirm the salt tolerance. The influence of mild salt stress (75 mM NaCl) on the growth parameters of selected clones was examined. At the end of this trial, the proline accumulation and sodium content in alfalfa shoots were also quantified. The results suggest an increased level of proline promotes salt tolerance. Medicago sativa L. var. icon is highly tolerant in comparison with the other varieties tested. In vitro selection of M. sativa L. varieties on salt-containing media allowed us to obtain clones with increased salinity tolerance.

Sign in / Sign up

Export Citation Format

Share Document