Effects of sire genotype and plane of nutrition on fascicular structure of M. longissimus thoracis et lumborum and its effect on eating quality

2006 ◽  
Vol 57 (6) ◽  
pp. 641 ◽  
Author(s):  
P. G. Allingham ◽  
G. E. Gardner ◽  
M. Taylor ◽  
R. S. Hegarty ◽  
G. S. Harper

The purpose of this study was to determine if estimated breeding value (EBV) of an animal’s sire and or the animal’s nutrition affected the structure of its M. longissimus thoracis et lumborum (LL) and, hence, the eating quality of meat derived from its carcass. Lambs were chosen based on the EBV of their sires in terms of post-weaning live weight (PWWT), post weaning fat at the C-site (PFAT), and post-weaning eye muscle depth (PEMD). Morphometric techniques were used to characterise muscle structure in terms of the distribution of intramuscular connective tissue; the variables together are called fascicular structure. Perimysial seam thickness and fascicular width were both influenced by sire estimated breeding values for PWWT, PFAT, and PEMD. Variation in fascicular structure was associated with an interaction between PEMD-EBV and PFAT-EBV of the sire. Fascicular width decreased with increased PEMD-EBV and increased with PFAT-EBV, but was not affected by PWWT-EBV. When the total seam thickness was adjusted to a common fascicular width, the lambs on a low plane of nutrition had relatively more intramuscular connective tissue than those on a high plane. The total seam thickness was negatively associated with PFAT-EBV and positively associated with PEMD-EBV. Warner Bratzler shear (WBS) peak force (PF) and initial yield were not associated with differences in sire EBV. The residual WBS shear force, peak force minus initial yield (PFIY), and compression values were negatively associated with nutrition but were positively associated with PWWT-EBV and PEMD-EBV of the sires. These latter 2 effects were moderated by nutrition. The data support the hypothesis that morphological characteristics of perimysium are genetically determined and nutritionally responsive. Variance in morphology accounted for some variance in the biophysical attributes of meat and may help explain why sheep with high muscling potential have tougher meat.

2009 ◽  
Vol 49 (3) ◽  
pp. 239 ◽  
Author(s):  
P. G. Allingham ◽  
W. Barris ◽  
A. Reverter ◽  
V. Hilsenstein ◽  
R. van de Ven ◽  
...  

The effects of sire genetics and growth path on the fascicular structure (FS) of lamb loin muscle (m. longissimus lumborum; LL) and the consequences for eating quality were studied in progeny (n = 299) of 20 Poll Dorset sires and Merino ewes. Sires were selected on the basis of their Australian sheep breeding values (ASBV) for post-weaning growth (PWWT), loin eye muscle depth (PEMD) and subcutaneous fat (PFAT). Growth of lambs was varied by time of weaning (early or late) and by feeding level within weaning group, where lambs were either fully fed throughout or were feed restricted for 55 days then re-alimented to achieve liveweight of ~45 kg at slaughter. The FS variables were determined in differentially stained histological sections of LL by using automated image segmentation and circle geometry methodology and were defined as percentage connective tissue, perimysial seam thickness and fascicular width. Sire ASBV was not a significant covariate to measures of FS, despite ASBV-related effects on growth, carcass leanness and fatness. Independent of weaning time, faster growth was induced by re-alimentation of feed-restricted lambs, resulting in LL with less connective tissue as a percentage of cross-sectional area and larger fascicles than for fully fed peers. The direction of the perimysial seam thickness response varied with treatment and time of weaning. Rapid growth of re-alimented early weaned restricted lambs resulted in thinner seams than for fully fed peers, whereas the opposite response was observed in the late-weaned lamb contrast. This suggests that the weight and age of the lamb at the time of nutritional compromise and re-alimentation are important factors in determining the thickness of perimysial connective-tissue seams. Whilst the evidence confirms differential accretion of the myofibrillar and connective tissues and despite reports of positive effects on tenderness brought about by such changes, variation in structural measures was not associated with variation in either objective measures (compression) or sensory scores for tenderness of the loin muscle. The data from the present study support the tenet that rapid growth before slaughter, combined with appropriate sire genetics, will not compromise the physical characteristics and sensory qualities of the meat.


2000 ◽  
Vol 84 (3) ◽  
pp. 275-284 ◽  
Author(s):  
G. E. Lobley ◽  
K. D. Sinclair ◽  
C. M. Grant ◽  
L. Miller ◽  
D. Mantle ◽  
...  

Eighteen pure-bred steers (live weight 350 kg) from each of two breeds, Aberdeen Angus (AA) and Charolais (CH), were split into three equal groups (six animals each) and offered three planes of nutrition during a 20-week period. The same ration formulation was offered to all animals with amounts adjusted at 3-week intervals to give predicted average weight gains of either 1·0 kg/d (M/M group) or 1·4 kg/d (H/H group). The remaining group (M/H) were offered the same amount of ration as the M/M group until 10 weeks before slaughter when the ration was increased to H. Data on animal performance, carcass characteristics and fibre-type composition in skeletal muscle are presented elsewhere (; ). On three occasions (17, 10 and 2 weeks before slaughter) the animals were transferred to metabolism stalls for 1 week, during which total urine collection for quantification of Nτ-methylhistidine (Nτ-MeH) elimination was performed for 4 d. On the last day, animals were infused for 11 h with [2H5] phenylalanine with frequent blood sampling (to allow determination of whole-body phenylalanine flux) followed by biopsies from m. longissimus lumborum and m. vastus lateralis to determine the fractional synthesis rate of mixed muscle protein. For both breeds, the absolute amount of Nτ-MeH eliminated increased with animal age or weight (P < 0·001) and was significantly greater for CH steers, at all intake comparisons, than for AA (P < 0·001). Estimates of fractional muscle breakdown rate (FBR; calculated from Nτ-MeH elimination and based on skeletal muscle as a fixed fraction of live weight) showed an age (or weight) decline for M/M and H/H groups of both breeds (P < 0·001). FBR was greater for the H/H group (P = 0·044). The M/H group also showed a lower FBR for the first two measurement periods (both at M intake) but increased when intake was raised to H. When allowance was made for differences in lean content (calculated from fat scores and eye muscle area in carcasses at the end of period 3), there were significant differences in muscle FBR with intake (P = 0·012) but not between breed. Whole-body protein flux (WBPF; g/d) based on plasma phenylalanine kinetics increased with age or weight (P < 0·001) and was similar between breeds. The WBPF was lower for M/M compared with H/H (P < 0·001) based on either total or per kg live weight0·75. Muscle protein fractional synthesis rate (FSR) declined with age for both breeds and tended to be higher at H/H compared with M intakes (intake × period effects, P < 0·05). Changing intake from M to H caused a significant increase (P < 0·001) in FSR. The FSR values for AA were significantly greater than for CH at comparable ages (P = 0·044). Although FSR and FBR responded to nutrition, these changes in protein metabolism were not reflected in differences in meat eating quality (Sinclair et al. 2000).


2016 ◽  
Vol 52 ◽  
pp. 82-94 ◽  
Author(s):  
A. Ye. Pochukalin

One of the ways of increasing level of animal economically useful traits is selection work with farm families. In pedigree cattle breeding of Ukraine families are a statistical component of breed genealogy. Among the main scientific works on working with families, it should be noted minimum number of female ancestors, proposed by D. T. Vinnichuk, to determine the breeding value, different categories, classification and techniques for evaluating related groups of females. The aim of our research was to analyse importance of farm families for genealogical structure of the breed. The research was on basis of data of primary breeding records at the herd of Volyn Beef cattle of “Zorya” breeding farm, Kovel district, Volyn region. Akula 102, Galka 37 and Galka 1537 families belonging to Krasavchyk 3004 bloodline, Smorodyna 613, Korona 2382 and Visla 1016 families – Tsebryk 3888 bloodline, Kalyna 212, Verba 1536 and Garna 536 families – Yamb 3066 bloodlines, Kazka 433, Galka 421 and Bystra 1124 families – Buinyi 3042 bloodline, Rozetka 1313, Arfa 599 and Bulana 943 families – Sonnyi-Kaktus 3307-9828 bloodline, and Palma 275, Desna 870 and Veselka 444 families – Mudryi 9100 bloodline were characterized. Belonging to a bloodline was determined by the father's side of female ancestors. Structural units of families: branches, branching with identifying the best individuals on breeding traits were submitted to identify the best combinations and successful use of closely related breeding. Comparing assessment of related groups of females on the main breeding traits belonging to Krasavchyk 3004 bloodline, it was noted that the cows of Akula 102 family predominated in live weight at 5 years’ age, milk ability and economic use duration, whereas the cows of Galka 1537 family – on traits of reproductive ability. Smorodyna 613 family of Tsebryk 3888 bloodline had high duration of economic use and cows’ live weight at 5 years’ age compared with Korana 2382 and Visla 1016 families with equal values of the exterior traits (height measures) and coefficient of reproductive ability. The families of Mudryi 9100 bloodline in terms of reproduction (calving interval, coefficient of reproductive ability) had the highest figures of cows’ milk ability and live weight. The cows of Bulana 943 family had a considerable predominance over representatives of Rozetka 1313 and Arfa 599 families of Sonnyi-Kaktus 3307-9828 bloodline by main economically useful traits. High indices of reproductive ability were noted in these families. Heifers of the families of Buinyi 3042 bloodline had high live weight at 18 months’ age at average values of milk ability and cows’ live weight at 5 years’ age. More equal figures of growth rate, exterior and economic use duration were observed in the cows of Kalyna 212, Verba 1536 and Garna 536 families of Yamb 3066 bloodline. Breeding by families in beef cattle breeding is an important element of selection, because it allows to evaluate not only related group of female ancestor, but also to analyse a successful combination with lines and purposeful use of closely related breeding by the best representatives of a breed.


Author(s):  
N.V. SIVKIN ◽  
N.V. STREKOZOV ◽  
V.I. CHINAROV

В симментальской породе предусматривается разведение скота, сбалансировано сочетающего молочную и мясную продуктивность. Однако в практике совершенствования племенных стад в подборах быков доминируют улучшатели удоя, что во многом предопределяет результаты селекции и продуктивный тип животных. Объектом нашего исследования стало стадо чистопородного симментальского скота в условиях стойловой системы беспривязного и привязного содержания коров. Для изучения эффективности использования быков-производителей разного племенного достоинства сформировали 2 опытные группы: I состояла из бычков, полученных от отцов с племенной ценностью (ПЦ) по удою 100 кг и более, а во II с ПЦ от 0 до 100 кг молока. Симментальские бычки, отобранные для контрольного убоя, достигали весовых кондиций 500 кг и более в 17,5 мес при среднесуточном приросте 911 г. При использовании на маточном поголовье быков-производителей с улучшающим эффектом по удою 100 кг и более, их сыновья (I группа), на фоне более высоких суточных приростов (на 30 г) и раннем возрасте достижения живой массы 500 кг (на 18 дней) имели массу и выход туши на 21,4 кг и 2,7 ниже, чем у бычков II группы. При формировании молочно-мясного типа быки-производители с умеренной племенной ценностью по удою обеспечивали получение потомства, сочетающего молочную и мясную продуктивность в экономически значимых пропорциях.The Simmental breed provides for the breeding of cattle that combines milk and meat productivity in a balanced proportion. However, in the practice of improving breeding herds, the selection of bulls is dominated by milk yield improvers, which largely determines the results of selection and the productive type of animals. The object of our research was a breeding herd of purebred Simmental cattle in variety feeding and housing practices. To study the effectiveness of using bulls-producers of different breeding values, 2 experimental groups were formed: I consisted of bulls received from fathers with a breeding value (BV) of milk yield 100 kg or more, and II with a BV from 0 to 100 kg of milk. Simmental bulls selected for control slaughter reached weight standards of 500 kg or more in 17.5 months with an average daily increase of 911 g. When used on breeding of bulls with an improving effect on the yield of 100 kg or more, their sons (group I), against the background of higher daily gains (30 g) and an early age of reaching a live weight of 500 kg (18 days), had a mass and carcass yield of 21.4 kg and 2.7 lower than that of group II bulls. When forming a dairy-meat type, producing bulls with a moderate breeding value for milk yield provided for the production of offspring that combined dairy and meat productivity in economically significant proportions.


1995 ◽  
Vol 60 (1) ◽  
pp. 117-124 ◽  
Author(s):  
J. A. Roden

AbstractStochastic simulation was used to compare the results of alternative breeding systems in a sheep population divided into 10 flocks of 120 ewes. The breeding systems compared were selection within closed flocks (CF), a closed nucleus system (CNS), an open nucleus system (ONS) and open nucleus systems with the selection of nucleus replacements being restricted to either nucleus born males (ONSRm) or nucleus born females (ONSRf). Selection was for a best linear unbiased prediction of breeding value for lamb live weight which had a heritability of 0·17. The open nucleus breeding systems (ONS, ONSRm, ONSRf) resulted in higher rates of genetic gain, more predictable selection responses and lower rates of inbreeding than either the closed nucleus system (CNS) or selection within closed flocks (CF). Initial genetic differences between flocks resulted in higher rates of genetic gain in the nucleus breeding systems due to the use of between flock genetic variance. In the ONS system up to 25% of nucleus sires and approximately 50% of nucleus dams were born in base flocks. Nevertheless if selection of either nucleus sires or dams was restricted to nucleus born animals there was very little change in genetic gain or rate of inbreeding.


1964 ◽  
Vol 6 (3) ◽  
pp. 301-308 ◽  
Author(s):  
Henning E. Nielsen

Sixteen blocks of eight littermate pigs were weaned at 3 weeks of age and each divided into four groups. Various levels of nutrition were applied to the pigs so that the four groups reached 20 kg. at 59, 68, 80 and 91 days of age, respectively.Only small differences were observed between groups in feed conversion ratio to 20 kg.; however, Group 4 required more feed per kg. gain than the three other groups.During the second period (20–90 kg.) the pigs in each group were divided into two sub-groups, which were on a high and a low plane of nutrition respectively. In both sub-groups the type of feeding before 20 kg. influenced the average daily gain and feed conversion ratio. The older the pigs were at 20 kg. the higher the daily gain and the lower the feed conversion ratio in the following period. There was an increase in daily feed intake with increased age at 20 kg. for pigs fed ad lib. during the second period.An increase in age at 20 kg. caused a significant decrease in thickness of backfat, a greater area of eye muscle and a smaller area of fat overlying muscle. For pigs fed ad lib. the area of eye muscle was significantly increased by a higher age at 20 kg. live-weight.


1965 ◽  
Vol 7 (2) ◽  
pp. 153-164 ◽  
Author(s):  
R. S. Barber ◽  
R. Braude ◽  
Zena D. Hosking ◽  
K. G. Mitchell

SUMMARY1. Seventy-two individually fed pigs on six treatments were used to determine the effects on performance and in particular on carcass quality of (a) giving quantities of liquid skim-milk (preserved with formalin) in excess of those normally recommended; (b) replacing meal by skim-milk in the ration on a calculated dry-matter basis (1 gal. skim-milk equivalent to 1 · 1 lb. meal) as compared with a calculated energy basis (1 gal. skim-milk equivalent to 1 · 3 lb. meal) and (c) giving skim-milk for only a part of the growing period instead of throughout. The experimental period continued from 9–10 weeks of age until slaughter at an average live-weight of 135 lb.2. Extensive carcass measurements were taken and in addition assessment of the carcasses was made by a representative of the County Quality Pork Association and by an expert pork butcher.3. Carcasses from pigs given an all-meal diet were comparable in all respects with those from pigs given skim-milk throughout up to a maximum of either 5¼ or 10½ pints per pig per day.4. Carcasses from pigs given the highest level of skim-milk (maximum of 15¾ pints per pig per day) were superior in many respects to those on any of the other treatments, but the possibility that this was due to their slower rate of growth rather than to the high level of milk per se, is discussed.5. Pigs given skim-milk as replacement of part of their meal allowance on a calculated dry matter basis grew significantly faster than those in which the replacement was made on a calculated energy basis or than those given an all-meal diet. The importance is stressed of ensuring that in assessing the value of skim-milk for pigs the caloric value of the rations being compared is similar. The need for experiments based on actual determinations of the caloric value of the rations used is also emphasised.6. While the performance of pigs given skim-milk up to 75 lb. live-weight only was similar to that of pigs given skim-milk throughout, evidence was obtained that the quality of their carcasses was slightly inferior.7. There was some suggestion that as the level of skim-milk in the diet increased an eye muscle of a rather paler colour was produced.


1984 ◽  
Vol 38 (1) ◽  
pp. 33-44 ◽  
Author(s):  
I. A. Wright ◽  
A. J. F. Russel

ABSTRACTA number of possible indices of body composition (live weight, skeletal size, total body water as estimated by deuterium oxide dilution, blood and red cell volumes as estimated by Evans Blue dilution, ultrasonic measurements of subcutaneous fat depth and eye-muscle area, and body condition scoring) was examined using 73 non-pregnant, non-lactating, mature cows of Hereford × Friesian, Blue-Grey, British Friesian, Galloway and Luing genotypes, ranging in body condition score from 0·75 to 4·5. Direct measurements of body composition in terms of water, fat, protein and ash were made following slaughter.Live weight, deuterium oxide dilution, ultrasonic measurements of subcutaneous fat depth and eyemuscle area, and body condition score were all considered to be potentially useful predictors of body composition. Combinations of techniques offered better predictions than did any single index. Using a combination of measurements it was possible to predict body fat and protein with a residual s.d. of 13·1 kg and 3·15 kg respectively. Breed differences in the partition of fat among the main adipose tissue depots necessitated the development of specific prediction equations for body fat based on condition score and subcutaneous fat depth for different breeds. Equations remain to be developed for predicting body composition in cows in different physiological states.


1958 ◽  
Vol 25 (2) ◽  
pp. 181-190 ◽  
Author(s):  
R. Braude ◽  
Pamela M. Clarke ◽  
K. G. Mitchell ◽  
A. S. Cray ◽  
A. Franke ◽  
...  

1. Results are reported of an experiment carried out under commercial conditions to obtain information on the comparative value of dried skim-milk and white fish meal as protein supplements for fattening pigs.2. There were four treatments. Control pigs (i) received a meal mixture containing 10% white fish meal, reduced to 7% from 130 lb. live weight. In the other experimental groups, the fish meal was replaced by dried skim-milk, (ii) on a weight for weight basis, (iv) on a protein basis and (iii) intermediate between (ii) and (iv). All pigs were fed the meal dry and ad lib. up to a maximum of 6½ lb./head daily. Unrestricted water was available.There were five pens of nine group-fed pigs on each treatment, involving a total of 180 pigs. The pigs were on experiment from approximately 10 weeks of age to bacon weight. Comprehensive carcass measurements were made on all the pigs.3. The results showed that a significantly better rate of growth was obtained when the white fish meal was replaced by dried skim-milk on a weight for weight basis. Increasing the percentage of skim-milk in the diet up to 15% resulted in a slightly better performance of the pigs, but no further improvement was obtained by raising the level of skim-milk to 20%. These results were discussed in relation to an apparent set-back that the control pigs suffered at the start of the experimental period.4. The inclusion of dried skim-milk in the diet had an adverse effect on the carcass grading, particularly in so far as it resulted in a thicker layer of back fat over the loin as compared with that in the control pigs.5. There was also some indication that the carcasses from the animals given dried skim-milk had slightly thicker bellies and a smaller breadth of eye muscle than the carcasses of the control animals that received fish meal.6. The results have been compared with those of an earlier trial, which was in most details the same as that reported here, the main difference being that in the earlier experiment individual feeding and one breed of pig were used.


Author(s):  
A. Balnikov ◽  
I. Gridyushko ◽  
Yu. Kazutova ◽  
M. Mikhailova ◽  
E. Romanishko

Purpose: evaluation of pigs Yorkshire rocks and Landraz on PRKAG3, MC4R and MyOD1 markers.Materials and methods. The paper presents the analysis of the results of genetic testing, and determines the frequency of alleles and genotypes of Yorkshire and Landrace breeds at the State Enterprise ZhodinoAgroPlemElita" of the Minsk region of the Republic of Belarus by the genes-markers of productive traits: PRKAG3, MC4R and MYOD1. Zootechnical and molecular genetic methods were used in the research (PCR/PDRF). Snapshot analysis was developed for the genotyping of pigs to detect polymorphic markers of meat-feeding productivity of pigs on the Genas PRKAG3, MyOD1, MC4R. At the first stage, there was an assessment of tribal young (173 heads) on its own productivity in terms of indicators: the age of achieving the living mass of 100 kg, the average daily increase (d) from birth to the live weight of 100 kg, the length of the body (cm), the lifetime meat quality of the thickness of the scrape (mm), height of the longest back muscles (mm), the content of meat in the body (%). At the second stage of the research, the controlling fastenings of 200 goals of pigs and evaluation of feedst and meat qualities in the following indicators were carried out: the age of achieving a living mass of 100 kg (days), the average daily increase (d), feed consumption per 1 kg of growth (to. Units). Also determined: the length of the carcass (cm), the slaughter output (%), the thickness of the spick over 6-7 breast vertebrae (mm), the area of "muscular eye" (cm2), the mass of the rear third of the half carcass (kg), the flow of meat in the carcus (%). Then, genetic studies were compared with productivity indicators.Results. As a result of the research, it was found that in the tested animals, the frequency of the preferred allele I-PRKAG3 made 0.171-0.288, A-MC4R – 0.243-0.315, and C-MYOD1 – 0.064-0.477, respectively. The developed comprehensive system for assessing the breeding value of Yorkshire and Landrace pigs based on the marker genes PRKAG3, MC4R, and MYOD1 allowed us to identify the preferred alleles and determine correlation with productivity traits that provided increase in the average population values: for self-productivity - by 2-4%, for fattening traits - by 2.7-12.5%, for meat traits - by 2.8-34.4 %.Conclusion. The use of integrated system in pig breeding will speed up the selection work to increase the indicators of fattening and meat productivity of the created breeding herds of Yorkshire and Landrace pigs.


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