Anti-methanogenic advantage of biserrula (Biserrula pelecinus) over subterranean clover (Trifolium subterraneum) from in vitro fermentation is maintained across growth stages and cutting treatments

2019 ◽  
Vol 70 (3) ◽  
pp. 263
Author(s):  
Bidhyut Kumar Banik ◽  
Zoey Durmic ◽  
William Erskine ◽  
Clinton Revell
Keyword(s):  
Growth Stage ◽  
Nutritive Value ◽  
Subterranean Clover ◽  
In Vitro Study ◽  
Trifolium Subterraneum ◽  
Reproductive Stage ◽  
Growth Stages ◽  
Plant Collection ◽  
Grazing Systems

The annual forage legume biserrula (Biserrula pelecinus L.) offers a promising opportunity as a low-methanogenic, bioactive pasture for southern Australian grazing systems where subterranean clover (Trifolium subterraneum L.) is the dominant annual pasture legume. This in vitro study to assess methanogenic potential examined how growth stage and cutting of biserrula affect biomass, nutritive value and fermentative parameters including methanogenic potential compared with subterranean clover. Both species were grown in a glasshouse, where three growth-stage treatments comprised plant collection at vegetative, reproductive or maturity growth stages. Three cutting (simulated grazing) treatments included cutting at the vegetative or reproductive stage and an uncut control, with herbage collection at maturity. Methane production in biserrula was unaffected by the stage of growth. Other nutritive value and fermentative parameters varied significantly with growth stage, the highest fibre content and lowest crude protein (CP) being found at maturity. Regrowth from herbage cut at the reproductive stage had higher CP and lower biomass than other cut treatments. In biserrula, this regrowth also showed the highest fermentability of the three cutting treatments including elevated methane yield. Notwithstanding these treatment effects on fermentability, biserrula maintained its strong anti-methanogenic advantage over subterranean clover across all treatments, confirming its potential as an anti-methanogenic bioactive pasture.

Plant factors influencing the nutritive value of some temperate annual pasture species

1973 ◽  
Vol 13 (63) ◽  
pp. 404 ◽  
Author(s):  
JG McIvor ◽  
DF Smith
Keyword(s):  
Nutritive Value ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Growth Cycle ◽  
Matter Content ◽  
In Vitro Digestibility ◽  
Dry Matter Content ◽  
Hordeum Leporinum ◽  
Brome Grass

Changes in herbage production, dry matter content, nitrogen content and in vitro digestibility of two sown species-subterranean clover (Trifolium subterraneum) and annual ryegrass (Lolium rigidum) - and five common volunteer species-capeweed (Arctotheca calendula), musky crowfoot (Erodium moschatum), barley grass (Hordeum leporinum), ripgut brome grass (Bromus rigidus) and soft brome grass (Bromus mollis)-were measured throughout their growth cycle. The volunteer species compared favourably with the sown species for these measurements and should be capable of providing nutritious grazing for animals.

scholarly journals An Improved Protocol for Agrobacterium-Mediated Transformation in Subterranean Clover (Trifolium subterraneum L.)

2021 ◽  
Vol 22 (8) ◽  
pp. 4181
Author(s):  
Fernando Perez Rojo ◽  
Sumedha Seth ◽  
William Erskine ◽  
Parwinder Kaur
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Nutritive Value ◽  
Transformation Efficiency ◽  
Incubation Temperature ◽  
Agronomic Traits ◽  
Genetic Manipulation ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Factors Affecting

Subterranean clover (Trifolium subterraneum) is the most widely grown annual pasture legume in southern Australia. With the advent of advanced sequencing and genome editing technologies, a simple and efficient gene transfer protocol mediated by Agrobacterium tumefaciens was developed to overcome the hurdle of genetic manipulation in subterranean clover. In vitro tissue culture and Agrobacterium transformation play a central role in testing the link between specific genes and agronomic traits. In this paper, we investigate a variety of factors affecting the transformation in subterranean clover to increase the transformation efficiency. In vitro culture was optimised by including cefotaxime during seed sterilisation and testing the best antibiotic concentration to select recombinant explants. The concentrations for the combination of antibiotics obtained were as follows: 40 mg L−1 hygromycin, 100 mg L−1 kanamycin and 200 mg L−1 cefotaxime. Additionally, 200 mg L−1 cefotaxime increased shoot regeneration by two-fold. Different plant hormone combinations were tested to analyse the best rooting media. Roots were obtained in a medium supplemented with 1.2 µM IAA. Plasmid pH35 containing a hygromycin-resistant gene and GUS gene was inoculated into the explants with Agrobacterium tumefaciens strain AGL0 for transformation. Overall, the transformation efficiency was improved from the 1% previously reported to 5.2%, tested at explant level with Cefotaxime showing a positive effect on shooting regeneration. Other variables in addition to antibiotic and hormone combinations such as bacterial OD, time of infection and incubation temperature may be further tested to enhance the transformation even more. This improved transformation study presents an opportunity to increase the feeding value, persistence, and nutritive value of the key Australian pasture.

The in vitro inhibition of Phytophthora clandestina by some rhizobia and the possible role of Rhizobium trifolii in biological control of Phytophthora root rot of subterranean clover

1999 ◽  
Vol 50 (8) ◽  
pp. 1469 ◽  
Author(s):  
S. Simpfendorfer ◽  
T. J. Harden ◽  
G. M. Murray
Keyword(s):  
Biological Control ◽  
Root Rot ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Root Disease ◽  
Rhizobium Trifolii ◽  
Biological Control Agents ◽  
Control Activity ◽  
Phytophthora Root Rot

The interaction between 29 isolates of Rhizobium and the in vitro growth of 3 strains of Phytophthora clandestina was investigated to determine the potential of these bacteria as biological control agents against root rot of subterranean clover (Trifolium subterraneum L.). The biological control activity of Rhizobium on the severity of root disease in seedlings was also investigated under glasshouse conditions. Thirteen of the 29 Rhizobium isolates caused significant reductions in the hyphal growth of the 3 P. clandestina isolates examined. Inoculation of seedlings with Rhizobium trifolii reduced the severity of root disease by 14–58% with corresponding increases in dry matter production of 20–73%. These results indicate that Rhizobium species have potential as biological control agents against the root rot of T. subterraneum seedlings caused by P. clandestina.

Pasture legume species differ in their capacity to acidify soil

1998 ◽  
Vol 49 (1) ◽  
pp. 53 ◽  
Author(s):  
C. Tang ◽  
L. Barton ◽  
C. Raphael
Keyword(s):  
Acid Production ◽  
Dry Matter ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Growth Stages ◽  
Total Acid ◽  
Ash Alkalinity ◽  
Ornithopus Sativus ◽  
Excess Cations ◽  
Pasture Legume

The capacity of subterranean clover (Trifolium subterraneum L. cv. Clare), medic (Medicago murex Willd. cv. Zodiac), serradella (Ornithopus sativus Brot. line SP1/13), biserrula (Biserrula pelecinus L. line Mor99), and woolly clover (Trifolium tomentosum L.) to acidify soil under N2 fixation was compared in a pot experiment using a poorly buffered sandy soil. The amount of acid produced per kg shoot dry matter (specific acid production) varied betweefin species and with growth stages, ranging from 44 to 128 cmol/kg shoot. Subterranean clover and serradella acidied soil to a greater extent than woolly clover and medic, whereas biserrula acidified soil least. Irrespective of pasture species and growth stage, specific acid production correlated well with concentrations of excess cations and calcium in shoots. Furthermore, total excess cation, ash alkalinity, and calcium in shoots were all good indicators of total acid production across all of the species.

Seed yield and nutritive value of dry, mature subterranean clover (Trifolium subterraneum L.)

2001 ◽  
Vol 41 (2) ◽  
pp. 169 ◽  
Author(s):  
Y. J. Ru ◽  
J. A. Fortune
Keyword(s):  
Seed Yield ◽  
Dry Matter ◽  
Mineral Content ◽  
Nutritive Value ◽  
Subterranean Clover ◽  
Zinc Content ◽  
Trifolium Subterraneum ◽  
Crude Fibre ◽  
Dry Matter Digestibility ◽  
Wide Range

The nutritive value of 26 cultivars of dry, mature subterranean clover was evaluated at Shenton Park, Perth, Western Australia. The cultivars were divided into 3 maturity groups according to flowering time and each cultivar was sown in blocks comprising 4 replicates. The plots were grazed by sheep at 2-week intervals during the growing season. Dry mature plant material and soil were sampled in summer to examine the effect of grazing and cultivar on seed yield and nutritive value of feed residues. Cultivars heavily grazed in spring had a low herbage mass. There was no difference in seed yield and seed weight between heavily and lightly grazed cultivars. Dry matter digestibility and mineral content of dry residues was inconsistent for the 2 grazing treatments. The dry matter digestibility of dry, mature subterranean clover ranged from 40 to 56%, with a wide range of crude fibre, nitrogen and mineral content for the 26 cultivars. While most minerals in the dry residues were above the requirement for sheep, 7 cultivars had a zinc content less than the maintenance requirement for sheep. There was an imbalance for all cultivars in calcium: phosphorus with a range of 4–10: 1. Concurrent estimates on the yield and composition of seed indicated that seed can be resource of minerals for grazing animals in summer. Most cultivars had a seed yield over 100 g/m2 with that of 9 cultivars being over 130 g/m2. Seed was rich in nitrogen, sodium, phosphorus, potassium, magnesium, zinc and copper, and poor in sodium, calcium and manganese. However, there were no cultivars with an appropriate ratio of calcium and phosphorus. The imbalance in nitrogen and sulfur was a result of high nitrogen content with the ratio ranging from 19: 1 to 29: 1.

Variation in salinity tolerance, early shoot mass and shoot ion concentrations within Lotus tenuis: towards a perennial pasture legume for saline land

2010 ◽  
Vol 61 (5) ◽  
pp. 379 ◽  
Author(s):  
N. L. Teakle ◽  
A. Snell ◽  
D. Real ◽  
E. G. Barrett-Lennard ◽  
T. D. Colmer
Keyword(s):  
Salt Tolerance ◽  
Salinity Tolerance ◽  
Nutritive Value ◽  
Screening Method ◽  
Trifolium Subterraneum ◽  
Dry Mass ◽  
Growth Stages ◽  
Lotus Tenuis ◽  
Pasture Legumes ◽  
Shoot Mass

Perennial legumes are needed for productive pastures in saline areas. We evaluated 40 lines of Lotus tenuis for tolerance to salinity at both germination and vegetative growth stages. Salt tolerance during the early vegetative stage was assessed in a sand-tank experiment with NaCl concentrations of 0–450 mm NaCl for 5 weeks. Most L. tenuis lines were more salt tolerant and had at least 50% lower shoot Na+ plus Cl– (% dry mass (DM)) compared with some other common pasture legumes, Medicago sativa, M. polymorpha and Trifolium subterraneum. Within L. tenuis significant variation in salt tolerance was found, with C50 values (concentrations of NaCl that decreased shoot dry matter to 50% of control) ranging from ~100 to 320 mm. Shoot concentrations of Cl–, Na+ and K+ did not always correlate with salt tolerance; some tolerant lines had low shoot Na+ and Cl– (and thus better nutritive value), while others tolerated high shoot Na+ and Cl–. We also found variation within L. tenuis for salt tolerance of seeds, with lines ranging from 0 to 70% germination after recovery from a prior exposure to 800 mm NaCl for 15 days. There was no relationship between salinity tolerance of scarified seeds and subsequent growth of seedlings; therefore, testing of seeds alone would not be an appropriate screening method for salt tolerance in L. tenuis. This study of 40 L. tenuis lines has shown significant genetic variation for salt tolerance within this species, and we have identified key lines with potential to be productive in saltland pasture systems.

Degradability of crude protein from clover herbages used in irrigated dairy production systems in northern Victoria

2001 ◽  
Vol 52 (3) ◽  
pp. 415 ◽  
Author(s):  
D. C. Cohen
Keyword(s):  
Protein Content ◽  
White Clover ◽  
Crude Protein ◽  
Nutritive Value ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Crude Protein Content ◽  
Persian Clover ◽  
Protein Supply ◽  
In Sacco

An in sacco (nylon bag) technique was used to estimate the degradability of dry matter (DM) and crude protein, and to estimate the effective rumen degradability of protein (ERDP), for 3 irrigated clover herbages. Pasture characteristics (nutritive values and leaf: stem ratios) were also described, and relationships to ERDP established. The nutritive value characteristics and degradabilities of white clover (Trifolium repens L.), Persian clover (Trifolium resupinatum L.), and subterranean clover (Trifolium subterraneum L.) were also compared for various regrowth periods (of 3, 4, 6, and 12 weeks). Nutritive value characteristics of clover herbages varied from 9.9 to 11.9 MJ/kg DM for metabolisable energy, 153 to 304 g/kg DM for crude protein, and 209 to 377 g/kg DM for neutral detergent fibre. The leaf: stem ratio correlated well with the crude protein content of the clovers, with herbages consisting of more leaf generally having superior crude protein content (R2 = 0.64, P < 0.001). The effective rumen degradability of protein for clover herbages ranged from 60 g/kg DM for mature (12 week regrowth) subterranean clover to 195 g/kg DM for vegetative (3 weeks regrowth) Persian clover. For clover herbages with a 3-week regrowth period, after initial cutting to 5 cm, and at ruminal outflow rates of 0.08/h, the effective rumen degradability of protein varied from 136 to 195 g/kg DM. A positive relationship between crude protein and ERDP (R2 = 0.82) suggested that ERDP could be estimated for clover herbages that have been previously assessed for crude protein content, obviating the need for in sacco studies. Calculated degradabilities were lower for all clovers when higher ruminal outflow rates were assumed. Using the metabolisable protein system, metabolisable protein supply and metabolisable protein in excess of animal requirements were calculated. Ruminal losses of nitrogen were also estimated for cows consuming white clover of varying regrowth periods in both early and late lactation. It was concluded that metabolisable protein supply is unlikely to limit production in these examples. At regrowth periods of 3 weeks, metabolisable protein contributions from microbial and dietary sources were similar, and twice that required by the animal. Ruminal losses of nitrogen were substantial and amounted to 66—23% of nitrogen intake. In the experiments reported here, if all of the energy required to excrete excess protein (as urea) could instead be used for the production of milk, cows may have produced 0.5mp;mdash;2.0 kg more milk per day. Such losses could potentially be reduced if the protein content and/or degradabilities of clover herbages were reduced, and/or energy rich supplements were offered.

Variation in nutritive value of plant parts of subterranean clover (Trifolium subterraneum L.)

2000 ◽  
Vol 40 (3) ◽  
pp. 397 ◽  
Author(s):  
Y. J. Ru ◽  
J. A. Fortune
Keyword(s):  
Nutritive Value ◽  
Nitrogen Concentration ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Vegetative Stage ◽  
Field Station ◽  
Leaf Petiole ◽  
Plant Parts ◽  
Dry Matter Digestibility ◽  
Lack Of Information

While it has been reported that leaves of subterranean clover are less digestible than stems, there is a lack of information on the variability of nutritive value of plant parts of subterranean clover. To determine the variation in nutritive value of leaf, petiole, stem and burr, an experiment with 26 cultivars of subterranean clover was conducted at Shenton Park Field Station, Perth, Western Australia. The cultivars were divided into 3 maturity groups according to flowering time and each cultivar was sown in blocks comprising 4 replicates. The plots were grazed by sheep at 2-weekly intervals. Plants were sampled at the vegetative stage before grazing and after the cessation of flowering. Dry matter digestibility (DMD) and nitrogen concentration of leaves, petioles, stems and burrs were determined. At the vegetative stage, there was no difference in DMD (P>0.05) among plant parts for most cultivars, and leaf had the highest (P<0.05) nitrogen concentration (4.8–5.4%). After the cessation of flowering, leaf had the highest DMD and nitrogen concentration (P<0.05). The DMD of plant parts differed significantly among cultivars (P<0.05). There was a slight decrease in DMD over time for leaves and a significant decrease in DMD for stems and petioles. These results suggest the main objective of grazing management of subterranean clover swards should be to increase the proportion of leaf material in the swards and that selection of leafy varieties by breeding could improve the late season digestibility of subterranean clover. Such management and breeding strategies would have value when the cultivars are in mixed pastures or used with supplements in summer.

Effect of calcium on the growth and virulence of Phytophthora clandestina

2000 ◽  
Vol 40 (1) ◽  
pp. 47 ◽  
Author(s):  
S. Simpfendorfer ◽  
T. J. Harden
Keyword(s):  
Fungal Pathogen ◽  
Mycelial Growth ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
In Vitro Growth ◽  
Seedling Biomass ◽  
Pathogenic Races ◽  
Glasshouse Experiment ◽  
The Individual

Calcium was found to significantly stimulate both the mycelial growth and virulence of Phytophthora clandestina, the major soilborne fungal pathogen of subterranean clover (Trifolium subterraneum L.). On average, the addition of calcium as either CaCO3 or CaCl2 to 3 artificial media increased the in vitro growth of 15 isolates from 20 to 135%. Calcium was also shown to increase the severity of root disease caused by 6 isolates of P. clandestina by up to 100% in a glasshouse experiment. The addition of Ca2+ as CaCl2 had a greater stimulatory effect on the virulence of P. clandestina towards cv. Woogenellup seedlings (up to 57% reduction in seedling biomass) than supplementing with CaCO3 (maximum 36% decrease). The virulence of 6 isolates from 3 different pathogenic races of P. clandestina were examined in the glasshouse experiment. Differences in the virulence of P. clandestina towards cv. Woogenellup seedlings was shown to be dependent on the individual isolate rather than the pathogenic race to which it is assigned.

scholarly journals Agronomic characteristics of annual Trifolium legumes and nutritive values as predicted by near-infrared reflectance (NIR) spectroscopy

2011 ◽  
Vol 62 (12) ◽  
pp. 1078 ◽  
Author(s):  
X. Li ◽  
R. L. Ison ◽  
R. C. Kellaway ◽  
C. Stimson ◽  
G. Annison ◽  
...  
Keyword(s):  
Organic Matter ◽  
Near Infrared ◽  
Spectral Characteristics ◽  
Nir Spectroscopy ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Infrared Reflectance ◽  
Near Infrared Reflectance ◽  
Stem Leaf

A range of annual legume genotypes comprising one line of Trifolium subterraneum, four lines of T. michelianum, 11 of T. resupinatum var. resupinatum, and one line of T. resupinatum var. majus were grown in glasshouses under temperature regimes of 10−15°C and 16−21°C. Dry matter (DM) weights of stem, leaf, and flower tissues were measured when plants had six nodes, at first flower appearance, and at senescence. All samples were scanned by near-infrared reflectance spectroscopy (NIRS). One-third of the samples, covering the range of spectral characteristics, were analysed for in vitro digestible organic matter (DOMD), organic matter, crude protein (CP), neutral detergent fibre (NDF), lignin, cellulose, and the hemicellulosic polysaccharide monomers arabinose, xylose, mannose, galactose, and rhamnose. These data were used to develop calibration equations from which the composition of the remaining samples was predicted by NIRS. The higher temperature resulted in plants reaching respective phenological stages earlier, but did not affect either DM yields of total plant, stem, leaf, and petiole tissues or the proportions of each fraction. In vitro DOMD and arabinose and galactose levels decreased, while lignin, cellulose, NDF, xylose, mannose, and rhamnose levels increased with advancing maturity. In vitro DOMD was positively associated with contents of CP, arabinose, galactose, and the arabinose/xylose ratio and was negatively associated with contents of lignin, cellulose, NDF, xylose, mannose, and rhamnose. Lignin contents were highly correlated with levels of both xylose and mannose. Stems were more digestible than leaves in subterranean clover and T. resupinatum var. majus. The study also demonstrated that NIRS can be used routinely as a quick, inexpensive, and reliable laboratory technique to predict feed components of annual Trifolium legumes.

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