Physiology and gene expression of the rice landrace Horkuch under salt stress

2011 ◽  
Vol 38 (4) ◽  
pp. 282 ◽  
Author(s):  
Laisa A. Lisa ◽  
Sabrina M. Elias ◽  
M. Sazzadur Rahman ◽  
Saima Shahid ◽  
Tetsushi Iwasaki ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Oryza Sativa L ◽  
Nacl Stress ◽  
Climatic Conditions ◽  
Shoot Biomass ◽  
Salt Tolerant ◽  
Potential Donor ◽  
Flag Leaves ◽  
South West Coast ◽  
Ros Scavengers

Good donors in breeding for salt tolerance are a prerequisite for food security under changing climatic conditions. Horkuch, a farmer-popular salt tolerant rice (Oryza sativa L.) variety from the south-west coast of Bangladesh was characterised up to maturity under NaCl stress, together with a modern variety (BRRI dhan41), a sensitive control (BRRI dhan29) and Pokkali, the salt-tolerant benchmark for rice. Horkuch had low reduction in shoot biomass, a low Na : K ratio in flag leaves, a low percent reduction in yield and good partitioning of Na in the older leaves, and maintained high levels of Ca and Mg in the flag leaves. In order to understand the physiology at the molecular level, the expression of salt-responsive genes was investigated using microarray analysis. Salt-stressed cDNA of Horkuch seedlings were hybridised with cDNA probes synthesised mainly from database sequences of Arabidopsis thaliana (L.) Heynh. The upregulated genes included transcription factors, signal transducers, metabolic enzymes, reactive oxygen species (ROS) scavengers, osmoprotectants and some specific salt-induced transcripts. An increase in expression of photosynthesis-related genes as well ROS scavengers suggested that this could be the reason for the better yield performance of Horkuch. The data therefore indicate Horkuch as a potential donor alternative to Pokkali in breeding programs for salt tolerance.

Physiological characterisation and fine mapping of a salt-tolerant mutant in rice (Oryza sativa)

2015 ◽  
Vol 42 (11) ◽  
pp. 1026 ◽  
Author(s):  
Ping Deng ◽  
Dan Jiang ◽  
Yanmin Dong ◽  
Xingyu Shi ◽  
Wen Jing ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Oryza Sativa L ◽  
Recessive Gene ◽  
Nacl Stress ◽  
Shoot Biomass ◽  
Salt Tolerant ◽  
Candidate Locus ◽  
F2 Population

Salt-tolerant mutants are valuable resources for basic and applied research on plant salt tolerance. Here, we report the isolation and characterisation of a salt-tolerant rice (Oryza sativa L.) mutant. This mutant was identified from an ethyl methanesulfonate-induced Nipponbare mutant library, designated as rice salt tolerant 1 (rst1). The rst1 mutant was tolerant to salt stress and showed significantly higher shoot biomass and chlorophyll content, but lower lipid peroxidation and electrolyte leakage under NaCl stress. The improved salt tolerance of this mutant may be due mainly to its enhanced ability to restrict Na+ accumulation in shoots under salt stress conditions. Genetic analysis indicated that the salt tolerance of the rst1 mutant was controlled by a single recessive gene. Quantitative trait locus (QTL) mapping for salt tolerance was performed using an F2 population of rst1 × Peiai 64. Two QTLs were detected, in which the locus on chromosome 6 was determined to be the candidate locus of the rst1 gene. The rst1 locus was subsequently shown to reside within a 270.4-kb region defined by the markers IM29432 and IM29702. This result will be useful for map-based cloning of the rst1 gene and for marker-assisted breeding for salt tolerance in rice.

scholarly journals Evaluation of salinity tolerance indices in North African barley accessions at reproductive stage

2019 ◽  
Vol 55 (No. 2) ◽  
pp. 61-69 ◽  
Author(s):  
Dorsaf Allel ◽  
Anis BenAmar ◽  
Mounawer Badri ◽  
Chedly Abdelly
Keyword(s):  
Salt Tolerance ◽  
Grain Yield ◽  
Salinity Tolerance ◽  
Selection Criteria ◽  
Reproductive Stage ◽  
Shoot Biomass ◽  
Salt Tolerant ◽  
North African ◽  
Grain Number ◽  
Selection Indices

Soil salinity is one of the main factors limiting cereal productivity in worldwide agriculture. Exploitation of natural variation in local barley germplasm is an effective approach to overcome yield losses. Three gene pools of North African Hordeum vulgare L. grown in Tunisia, Algeria and Egypt were evaluated at the reproductive stage under control and saline conditions. Assessment of stress tolerance was monitored using morphological, yield-related traits and phenological parameters of reproductive organs showing significant genetic variation. High heritability and positive relationships were found suggesting that some traits associated with salt tolerance could be used as selection criteria. The phenotypic correlations revealed that vegetative traits including shoot biomass, tiller number and leaf number along with yield-related traits such as spike number, one spike dry weight, grain number/plant and grain number/spike were highly positively correlated with grain yield under saline conditions. Hence, these traits can be used as reliable selection criteria to improve barley grain yield. Keeping a higher shoot biomass and longer heading and maturity periods as well as privileged filling ability might contribute to higher grain production in barley and thus could be potential target traits in barley crop breeding toward improvement of salinity tolerance. Multiple selection indices revealed that salt tolerance trait index provided a better discrimination of barley landraces allowing selection of highly salt-tolerant and highly productive genotypes under severe salinity level. Effective evaluation of salt tolerance requires an integration of selection indices to successfully identify and characterize salt tolerant lines required for valuable exploitation in the management of salt-affected areas.  

scholarly journals Physiological and Biochemical Responses to Salt Stress of Alfalfa Populations Selected for Salinity Tolerance and Grown in Symbiosis with Salt-Tolerant Rhizobium

Agronomy ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 569
Author(s):  
Annick Bertrand ◽  
Craig Gatzke ◽  
Marie Bipfubusa ◽  
Vicky Lévesque ◽  
Francois P. Chalifour ◽  
...  
Keyword(s):  
Amino Acids ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Water Content ◽  
Salinity Tolerance ◽  
Osmotic Adjustment ◽  
Recurrent Selection ◽  
Aromatic Amino Acids ◽  
Nacl Stress ◽  
Salt Tolerant

Alfalfa and its rhizobial symbiont are sensitive to salinity. We compared the physiological responses of alfalfa populations inoculated with a salt-tolerant rhizobium strain, exposed to five NaCl concentrations (0, 20, 40, 80, or 160 mM NaCl). Two initial cultivars, Halo (H-TS0) and Bridgeview (B-TS0), and two populations obtained after three cycles of recurrent selection for salt tolerance (H-TS3 and B-TS3) were compared. Biomass, relative water content, carbohydrates, and amino acids concentrations in leaves and nodules were measured. The higher yield of TS3-populations than initial cultivars under salt stress showed the effectiveness of our selection method to improve salinity tolerance. Higher relative root water content in TS3 populations suggests that root osmotic adjustment is one of the mechanisms of salt tolerance. Higher concentrations of sucrose, pinitol, and amino acid in leaves and nodules under salt stress contributed to the osmotic adjustment in alfalfa. Cultivars differed in their response to recurrent selection: under a 160 mM NaCl-stress, aromatic amino acids and branched-chain amino acids (BCAAs) increased in nodules of B-ST3 as compared with B-TS0, while these accumulations were not observed in H-TS3. BCAAs are known to control bacteroid development and their accumulation under severe stress could have contributed to the high nodulation of B-TS3.

scholarly journals Screening of Rice (Oryza sativa L.) Genotypes at Reproductive Stage for their Tolerance to Salinity

2018 ◽  
Vol 16 (1) ◽  
pp. 65-77
Author(s):  
M M Islam ◽  
M H Faruqe ◽  
M S Rana ◽  
M Akter ◽  
M A Karim
Keyword(s):  
Oryza Sativa ◽  
Salt Tolerance ◽  
Grain Yield ◽  
Oryza Sativa L ◽  
Saline Water ◽  
Coastal Zones ◽  
Salt Tolerant ◽  
Randomized Design ◽  
Rice Genotypes ◽  
Four Levels

Soil salinity is one of the most devastating environmental stresses for rice production in the coastal areas of Bangladesh. Improvement in salt tolerance of rice is an important way for the economic utilization of coastal zones. An experiment was conducted at the vinyl house of the Department of Agronomy, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Bangladesh during December 2016 to April 2017 to determine the effects of different salinity levels on the yield and yield components of some rice (Oryza sativa L.) genotype sand finally, screening of rice genotypes for salt tolerance. The experiment containing four treatments was laid out in a complete randomized design with five replications. The treatments were four levels of saline water with electrical conductivities at control (0.3 dSm-1), 5, 10 and 15dSm-1. Based on screening at germination stage, relatively salt-tolerant eleven genotypes with two check varieties were used in this experiment. The study showed that increase in salinity level significantly decreased yield and yield contributing characters of rice. However, among the eleven, three genotypes (Chapali, Patnai 23 and Soloi) were considered as moderately salt tolerant at 15 dSm-1 on the basis of their yields and yield contributing characteristics such as plant height reduction, total tiller reduction, effective tiller reduction, reduction of fertile grains per panicle, grain yield and relative grain yield. Therefore, Chapali, Patnai 23 and Soloi might be recommended as moderately salt tolerant rice genotypes. The Agriculturists 2018; 16(1) 65-77

scholarly journals Identification and Validation a Major QTL from “Sea Rice 86” Seedlings Conferred Salt Tolerance

Agronomy ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 410 ◽  
Author(s):  
Fengling Wu ◽  
Jun Yang ◽  
Diqiu Yu ◽  
Peng Xu
Keyword(s):  
Salt Tolerance ◽  
Oryza Sativa L ◽  
Bulked Segregant Analysis ◽  
Indica Rice ◽  
Chromosome 1 ◽  
Saline Stress ◽  
Salt Tolerant ◽  
Rice Varieties ◽  
Target Region ◽  
High Salt Tolerance

Saline stress severely affects rice (Oryza sativa L.) growth and development and reduces crop yield. Therefore, developing salt-tolerant and high-yielding rice using quantitative trait loci (QTLs) and linkage markers is a priority for molecular breeding. Here, the indica rice Sea Rice 86 (SR86) seedlings showed higher tolerance than ordinary rice varieties in saline soil, and a dominant effect on salinity sensitivity was demonstrated by genetic analysis. We constructed bulked segregant analysis pools using F2 populations from parents Dianjingyou 1 as the recipient and SR86 as the donor. We identified a 2.78 Mb region on chromosome 1 as the candidate region. Using simple sequence repeat markers and substitution analysis, we mapped the target region within 5.49 cM in the vicinity of markers RM8904–RM493. We speculated that this QTL, named qST1.1, might contribute significantly to the salt tolerance of SR86. The high salt tolerance of introgression lines obtained by marker assistant selection (MAS) confirmed that the qST1.1 region was associated with salinity tolerance. This newly-discovered QTL will be helpful for the analysis of the salt-tolerant mechanism of rice and breeding high-quality rice varieties using MAS.

scholarly journals K+ Efflux and Retention in Response to NaCl Stress Do Not Predict Salt Tolerance in Contrasting Genotypes of Rice (Oryza sativa L.)

PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e57767 ◽  
Author(s):  
Devrim Coskun ◽  
Dev T. Britto ◽  
Yuel-Kai Jean ◽  
Imtiaz Kabir ◽  
Inci Tolay ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Salt Tolerance ◽  
Oryza Sativa L ◽  
Nacl Stress

scholarly journals Transcriptional profiling of two contrasting genotypes uncovers molecular mechanisms underlying salt tolerance in alfalfa

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rakesh Kaundal ◽  
Naveen Duhan ◽  
Biswa R. Acharya ◽  
Manju V. Pudussery ◽  
Jorge F. S. Ferreira ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Molecular Mechanisms ◽  
Transcriptional Profiling ◽  
Shoot Biomass ◽  
Tolerance Index ◽  
Valuable Insight ◽  
Salt Tolerant ◽  
Shoot Number ◽  
Salt Tolerance Index ◽  
High Level

AbstractAlfalfa is an important forage crop that is moderately tolerant to salinity; however, little is known about its salt-tolerance mechanisms. We studied root and leaf transcriptomes of a salt-tolerant (G03) and a salt-sensitive (G09) genotype, irrigated with waters of low and high salinities. RNA sequencing led to 1.73 billion high-quality reads that were assembled into 418,480 unigenes; 35% of which were assigned to 57 Gene Ontology annotations. The unigenes were assigned to pathway databases for understanding high-level functions. The comparison of two genotypes suggested that the low salt tolerance index for transpiration rate and stomatal conductance of G03 compared to G09 may be due to its reduced salt uptake under salinity. The differences in shoot biomass between the salt-tolerant and salt-sensitive lines were explained by their differential expressions of genes regulating shoot number. Differentially expressed genes involved in hormone-, calcium-, and redox-signaling, showed treatment- and genotype-specific differences and led to the identification of various candidate genes involved in salinity stress, which can be investigated further to improve salinity tolerance in alfalfa. Validation of RNA-seq results using qRT-PCR displayed a high level of consistency between the two experiments. This study provides valuable insight into the molecular mechanisms regulating salt tolerance in alfalfa.

scholarly journals Unfolded protein response in rice (Oryza sativa L.) varieties with different level of salt stress tolerance

2021 ◽  
Vol 26 (3) ◽  
pp. 151
Author(s):  
Galang Rizki Ramadhan ◽  
Sholeh Avivi ◽  
Bambang Sugiharto ◽  
Wahyu Indra Duwi Fanata
Keyword(s):  
Endoplasmic Reticulum ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Unfolded Protein Response ◽  
Oryza Sativa L ◽  
Stress Conditions ◽  
Salt Tolerant ◽  
Unfolded Protein ◽  
The Unfolded Protein Response ◽  
Protein Response

Plants activate the unfolded protein response as part of cellular adaptation, thereby maintaining the endoplasmic reticulum homeostasis during external stresses exposure. In this study, we examined the relationship between the degree of salt tolerance and unfolded protein response-related gene expression in India salt-tolerant Pokkali and INPARI 35 varieties compared to the Indica salt-sensitive counterpart IR64 and INPARI 4 varieties.  Our result showed that the salt tolerance of Pokkali and INPARI 35 had been confirmed by their higher survival rate, higher chlorophyll content, lower electrolyte leakage, and lower H2O2 and malondialdehyde content under salt stress conditions. Furthermore, the expression of unfolded protein response genes was highest in INPARI 35, whereas IR64 and INPARI 4 exhibited low gene induction during endoplasmic reticulum stress conditions. Among the four examined varieties the salt tolerant Pokkali surprisingly showed the lowest induction of all examined unfolded protein response-related genes. These results indicated the possibility that unfolded protein response supports the rice plant for adapting to the saline environment.

scholarly journals SCREENING OF SALT TOLERANT RHIZOBIA FROM GROUNDNUT IN CUDDALORE DISTRICT OF TAMIL NADU, INDIA

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
N. Pandeeswari ◽  
K. Sivakumar
Keyword(s):  
Salt Tolerance ◽  
Land Surface ◽  
Tamil Nadu ◽  
Crop Production ◽  
Climatic Conditions ◽  
Irrigated Agriculture ◽  
Salt Tolerant ◽  
Salinity Response ◽  
The World ◽  
Salt Affected Soils

Salinity seriously constrains crop yield in irrigated agriculture throughout the world. Also, salinity is a serious threat to agriculture in arid and semi arid regions. Nearly 10 % of the world’s land surface can be classified as endangered by salinity. Salinity in the soil and irrigation water is an environmental problem and a major constraint for crop production. Currently, 20 % of the world’s cultivated land is affected by salinity, which results in the loss of 50 % of agricultural yield. At present, there are nearly 954 million hectares of saline soils on the earth’s surface. All these salt affected soils are distributed throughout the world. The salinity response of legumes in general varies greatly depending on factors like climatic conditions, soil properties, salt tolerance and the stages of crop growth. Successful cultivation of legumes can be achieved by the selection and/or development of a salt-tolerant legume Rhizobium combination although high salinities are known to affect rhizobial activities. The aim of present study is the effect of strains of salt tolerant Rhizobia on IAA, EPS, nodule ARA activity, Nitrogen content, leghemoglobin content, siderophore production, IAR and salt concentration of Groundnut on coastal area of Cuddalore District of Tamil Nadu. The GNR CD-4 is the effect salt tolerance strain compared to other strains.

Sign in / Sign up

Export Citation Format

Share Document