Protein S-nitrosylation in plant abiotic stresses

2020 ◽  
Vol 47 (1) ◽  
pp. 1 ◽  
Author(s):  
Jing Zhang ◽  
Weibiao Liao
Keyword(s):  
Nitric Oxide ◽  
Abiotic Stress ◽  
Abiotic Stresses ◽  
Protein S ◽  
Plant Response ◽  
Covalent Attachment ◽  
Post Translational Modification ◽  
Current State ◽  
Physiological And Biochemical ◽  
Translational Level

Plants are exposed to various environmental stresses that affect crop growth and production. During stress, various physiological and biochemical changes including the production of nitric oxide (NO), take place. It is clear that NO could work through either transcriptional or post-translational level. The redox-based post-translational modification S-nitrosylation – the covalent attachment of an NO moiety to a reactive cysteine thiol of a protein to form an S-nitrosothiol (SNO) – has attracted increasing attention in the regulation of abiotic stress signalling. So far, the relevance of S-nitrosylation of certain proteins has been investigated under abiotic stress. In this work, we focus on the current state of knowledge regarding S-nitrosylation in plants under abiotic stress, and provide a better understanding of the relevance of S-nitrosylation in plant response to abiotic stress.

scholarly journals Antioxidant Properties of S-Nitrosoglutathione and Nanotechnologies

Proceedings ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 15
Author(s):  
Parent ◽  
Zhou ◽  
Bonetti ◽  
Perrin-Sarrado ◽  
Lartaud ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Cardiovascular Diseases ◽  
Sustained Release ◽  
Antioxidant Properties ◽  
Protein S ◽  
Post Translational Modification ◽  
Antioxidant Power

Cardiovascular diseases are associated with oxidative stress and a reduced bioavailability of nitric oxide (NO). To counteract both processes, the administration of S-nitrosoglutathione (GSNO) can be envisaged. GSNO is able to induce protein S-nitrosation (Pr-SNO), which is a post-translational modification of proteins, participating in the storage of NO in tissues, and protect thiol functions from oxidation. However, GSNO antioxidant power is poorly studied, which is probably linked to its low stability. This low stability can be addressed by nanotechnologies that will increase GSNO protection and provide a sustained release of the drug.

scholarly journals Tomato Root Growth Inhibition by Salinity and Cadmium Is Mediated By S-Nitrosative Modifications of ROS Metabolic Enzymes Controlled by S-Nitrosoglutathione Reductase

Biomolecules ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 393 ◽  
Author(s):  
Jedelská ◽  
Kraiczová ◽  
Berčíková ◽  
Činčalová ◽  
Luhová ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Root Growth ◽  
Growth Inhibition ◽  
Protein S ◽  
Cadmium Stress ◽  
Root Growth Inhibition ◽  
Post Translational Modification ◽  
Tomato Root ◽  
Wild Tomato Species ◽  
Ros Metabolism

S-nitrosoglutathione reductase (GSNOR) exerts crucial roles in the homeostasis of nitric oxide (NO) and reactive nitrogen species (RNS) in plant cells through indirect control of S-nitrosation, an important protein post-translational modification in signaling pathways of NO. Using cultivated and wild tomato species, we studied GSNOR function in interactions of key enzymes of reactive oxygen species (ROS) metabolism with RNS mediated by protein S-nitrosation during tomato root growth and responses to salinity and cadmium. Application of a GSNOR inhibitor N6022 increased both NO and S-nitrosothiol levels and stimulated root growth in both genotypes. Moreover, N6022 treatment, as well as S-nitrosoglutathione (GSNO) application, caused intensive S-nitrosation of important enzymes of ROS metabolism, NADPH oxidase (NADPHox) and ascorbate peroxidase (APX). Under abiotic stress, activities of APX and NADPHox were modulated by S-nitrosation. Increased production of H2O2 and subsequent oxidative stress were observed in wild Solanum habrochaites, together with increased GSNOR activity and reduced S-nitrosothiols. An opposite effect occurred in cultivated S. lycopersicum, where reduced GSNOR activity and intensive S-nitrosation resulted in reduced ROS levels by abiotic stress. These data suggest stress-triggered disruption of ROS homeostasis, mediated by modulation of RNS and S-nitrosation of NADPHox and APX, underlies tomato root growth inhibition by salinity and cadmium stress.

scholarly journals An Abiotic Stress Responsive U-Box E3 Ubiquitin Ligase Is Involved in OsGI-Mediating Diurnal Rhythm Regulating Mechanism

Plants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1071
Author(s):  
Yo-Han Yoo ◽  
Xu Jiang ◽  
Ki-Hong Jung
Keyword(s):  
Abiotic Stress ◽  
Diurnal Rhythm ◽  
Abiotic Stresses ◽  
Phylogenomic Analysis ◽  
Expression Data ◽  
Class Iii ◽  
Post Translational Modification ◽  
Essential Information ◽  
Microarray Expression Data ◽  
Microarray Expression

The plant U-box (PUB) protein is the E3 ligase that plays roles in the degradation or post-translational modification of target proteins. In rice, 77 U-box proteins were identified and divided into eight classes according to the domain configuration. We performed a phylogenomic analysis by integrating microarray expression data under abiotic stress to the phylogenetic tree context. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) expression analyses identified that eight, twelve, and eight PUB family genes are associated with responses to drought, salinity, and cold stress, respectively. In total, 16 genes showed increased expression in response to three abiotic stresses. Among them, the expression of OsPUB2 in class II and OsPUB33, OsPUB39, and OsPUB41 in class III increased in all three abiotic stresses, indicating their involvement in multiple abiotic stress regulation. In addition, we identified the circadian rhythmic expression for three out of 16 genes responding to abiotic stress through meta-microarray expression data analysis. Among them, OsPUB4 is predicted to be involved in the rice GIGANTEA (OsGI)-mediating diurnal rhythm regulating mechanism. In the last, we constructed predicted protein-protein interaction networks associated with OsPUB4 and OsGI. Our analysis provides essential information to improve environmental stress tolerance mediated by the PUB family members in rice.

New Insights into the Functional Role of Nitric Oxide and Reactive Oxygen Species in Plant Response to Biotic and Abiotic Stress Conditions

2021 ◽  
pp. 215-235
Author(s):  
Mounira Chaki ◽  
Juan C. Begara-Morales ◽  
Raquel Valderrama ◽  
Lorena Aranda-Caño ◽  
Juan B. Barroso
Keyword(s):  
Nitric Oxide ◽  
Reactive Oxygen Species ◽  
Abiotic Stress ◽  
Functional Role ◽  
Reactive Oxygen ◽  
Plant Response ◽  
Stress Conditions ◽  
Oxygen Species ◽  
Biotic And Abiotic Stress

scholarly journals Differential expression of AtWAKL10 in response to nitric oxide suggests a putative role in biotic and abiotic stress responses

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7383
Author(s):  
Phearom Bot ◽  
Bong-Gyu Mun ◽  
Qari Muhammad Imran ◽  
Adil Hussain ◽  
Sang-Uk Lee ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Abiotic Stress ◽  
Pseudomonas Syringae ◽  
Stress Responses ◽  
Abiotic Stresses ◽  
Nitrosative Stress ◽  
Abiotic Stress Tolerance ◽  
Biotic And Abiotic Stress ◽  
Transcript Accumulation

Plant defense against pathogens and abiotic stresses is regulated differentially by communicating signal transduction pathways in which nitric oxide (NO) plays a key role. Here, we show the biological role of Arabidopsis thaliana wall-associated kinase (AtWAK) Like10 (AtWAKL10) that exhibits greater than a 100-fold change in transcript accumulation in response to the NO donor S-nitroso-L-cysteine (CysNO), identified from high throughput RNA-seq based transcriptome analysis. Loss of AtWAKL10 function showed a similar phenotype to wild type (WT) with, however, less branching. The growth of atwakl10 on media supplemented with oxidative or nitrosative stress resulted in differential results with improved growth following treatment with CysNO but reduced growth in response to S-nitrosoglutatione (GSNO) and methyl-viologen. Further, atwakl10 plants exhibited increased susceptibility to virulent Pseudomonas syringae pv tomato (Pst) DC3000 with a significant increase in pathogen growth and decrease in PR1 transcript accumulation compared to WT overtime. Similar results were found in response to Pst DC3000 avrB, resulting in increased cell death as shown by increased electrolyte leakage in atwakl10. Furthermore, atwakl10 also showed increased reactive oxygen species accumulation following Pst DC3000 avrB inoculation. Promoter analysis of AtWAKL10 showed transcription factor (TF) binding sites for biotic and abiotic stress-related TFs. Further investigation into the role of AtWAKL10 in abiotic stresses showed that following two weeks water-withholding drought condition most of the atwakl10 plants got wilted; however, the majority (60%) of these plants recovered following re-watering. In contrast, in response to salinity stress, atwakl10 showed reduced germination under 150 mM salt stress compared to WT, suggesting that NO-induced AtWAKL10 differentially regulates different abiotic stresses. Taken together, this study further elucidates the importance of NO-induced changes in gene expression and their role in plant biotic and abiotic stress tolerance.

Roles of Nitric Oxide in Conferring Multiple Abiotic Stress Tolerance in Plants and Crosstalk with Other Plant Growth Regulators

2021 ◽  
Author(s):  
Rajesh Kumar Singhal ◽  
Hanuman Singh Jatav ◽  
Tariq Aftab ◽  
Saurabh Pandey ◽  
Udit Nandan Mishra ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Abiotic Stress ◽  
Plant Growth ◽  
Stress Tolerance ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Abiotic Stress Tolerance

scholarly journals Identification and Characterization of Abiotic Stress Responsive CBL-CIPK Family Genes in Medicago

2021 ◽  
Vol 22 (9) ◽  
pp. 4634
Author(s):  
Wenxuan Du ◽  
Junfeng Yang ◽  
Lin Ma ◽  
Qian Su ◽  
Yongzhen Pang
Keyword(s):  
Abiotic Stress ◽  
Abiotic Stresses ◽  
Expression Patterns ◽  
Interacting Protein ◽  
Forage Crop ◽  
Cis Acting ◽  
Protein Motifs ◽  
Plant Signal Transduction ◽  
Identification And Characterization

The calcineurin B-like protein (CBL) and CBL-interacting protein kinase (CIPK) play important roles in plant signal transduction and response to abiotic stress. Plants of Medicago genus contain many important forages, and their growth is often affected by a variety of abiotic stresses. However, studies on the CBL and CIPK family member and their function are rare in Medicago. In this study, a total of 23 CBL and 58 CIPK genes were identified from the genome of Medicago sativa as an important forage crop, and Medicaog truncatula as the model plant. Phylogenetic analysis suggested that these CBL and CIPK genes could be classified into five and seven groups, respectively. Moreover, these genes/proteins showed diverse exon-intron organizations, architectures of conserved protein motifs. Many stress-related cis-acting elements were found in their promoter region. In addition, transcriptional analyses showed that these CBL and CIPK genes exhibited distinct expression patterns in various tissues, and in response to drought, salt, and abscisic acid treatments. In particular, the expression levels of MtCIPK2 (MsCIPK3), MtCIPK17 (MsCIPK11), and MtCIPK18 (MsCIPK12) were significantly increased under PEG, NaCl, and ABA treatments. Collectively, our study suggested that CBL and CIPK genes play crucial roles in response to various abiotic stresses in Medicago.

scholarly journals Metabolomics-Guided Elucidation of Plant Abiotic Stress Responses in the 4IR Era: An Overview

Metabolites ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 445
Author(s):  
Morena M. Tinte ◽  
Kekeletso H. Chele ◽  
Justin J. J. van der Hooft ◽  
Fidele Tugizimana
Keyword(s):  
Machine Learning ◽  
Abiotic Stress ◽  
Stress Responses ◽  
Abiotic Stresses ◽  
Industrial Revolution ◽  
Chemical Space ◽  
Big Data Analytics ◽  
Plant Responses ◽  
Next Generation ◽  
Computational Tools

Plants are constantly challenged by changing environmental conditions that include abiotic stresses. These are limiting their development and productivity and are subsequently threatening our food security, especially when considering the pressure of the increasing global population. Thus, there is an urgent need for the next generation of crops with high productivity and resilience to climate change. The dawn of a new era characterized by the emergence of fourth industrial revolution (4IR) technologies has redefined the ideological boundaries of research and applications in plant sciences. Recent technological advances and machine learning (ML)-based computational tools and omics data analysis approaches are allowing scientists to derive comprehensive metabolic descriptions and models for the target plant species under specific conditions. Such accurate metabolic descriptions are imperatively essential for devising a roadmap for the next generation of crops that are resilient to environmental deterioration. By synthesizing the recent literature and collating data on metabolomics studies on plant responses to abiotic stresses, in the context of the 4IR era, we point out the opportunities and challenges offered by omics science, analytical intelligence, computational tools and big data analytics. Specifically, we highlight technological advancements in (plant) metabolomics workflows and the use of machine learning and computational tools to decipher the dynamics in the chemical space that define plant responses to abiotic stress conditions.

scholarly journals Evaluation of Indigenous Olive Biocontrol Rhizobacteria as Protectants against Drought and Salt Stress

2021 ◽  
Vol 9 (6) ◽  
pp. 1209
Author(s):  
Nuria Montes-Osuna ◽  
Carmen Gómez-Lama Cabanás ◽  
Antonio Valverde-Corredor ◽  
Garikoitz Legarda ◽  
Pilar Prieto ◽  
...  
Keyword(s):  
Salt Stress ◽  
Abiotic Stresses ◽  
Biochemical Parameters ◽  
Biocontrol Agent ◽  
Negative Effects ◽  
Experimental Conditions ◽  
Acds Gene ◽  
Drought And Salt Stress ◽  
Physiological And Biochemical

Stress caused by drought and salinity may compromise growth and productivity of olive (Olea europaea L.) tree crops. Several studies have reported the use of beneficial rhizobacteria to alleviate symptoms produced by these stresses, which is attributed in some cases to the activity of 1-aminocyclopropane-1-carboxylic acid deaminase (ACD). A collection of beneficial olive rhizobacteria was in vitro screened for ACD activity. Pseudomonas sp. PICF6 displayed this phenotype and sequencing of its genome confirmed the presence of an acdS gene. In contrast, the well-known root endophyte and biocontrol agent Pseudomonas simiae PICF7 was defective in ACD activity, even though the presence of an ACD-coding gene was earlier predicted in its genome. In this study, an unidentified deaminase was confirmed instead. Greenhouse experiments with olive ‘Picual’ plants inoculated either with PICF6 or PICF7, or co-inoculated with both strains, and subjected to drought or salt stress were carried out. Several physiological and biochemical parameters increased in stressed plants (i.e., stomatal conductance and flavonoids content), regardless of whether or not they were previously bacterized. Results showed that neither PICF6 (ACD positive) nor PICF7 (ACD negative) lessened the negative effects caused by the abiotic stresses tested, at least under our experimental conditions.

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