82 Compensation of the Growth and Development of Individually Transferred Bovine Bisected Embryos

2018 ◽  
Vol 30 (1) ◽  
pp. 180
Author(s):  
E. I. Schild ◽  
A. E. Ynsaurralde-Rivolta ◽  
S. López-Valiente ◽  
S. Maresca ◽  
C. Munar ◽  
...  
Keyword(s):  
Standard Deviation ◽  
Surgical Technique ◽  
Growth And Development ◽  
Size Difference ◽  
Femur Length ◽  
Biparietal Diameter ◽  
Original Number ◽  
Recorded Data ◽  
And Control ◽  
Number Of Cells

Embryo bisection has been used in cattle for decades to multiply embryos. Recent articles assert that each bisected hemi-embryo continues its growth and differentiation without regenerating the original number of cells, producing smaller embryos. This size difference has been verified until Day 17, when the embryo has already elongated. The objective of this research was to evaluate fetal morphometric differences between embryos and hemi-embryos during the intrauterine period and at birth. For this purpose, 6 Angus cows were superovulated using the FSH/LH protocol (Pluset®, Hertape Calier, Minas Gerais, Brazil) and all were artificially inseminated with the sire ‘Las Lilas 1931 Líder Max’. At Day 7 post-AI, 45 embryos were recovered using a non-surgical technique. In the laboratory, 30 of them were bisected under a stereoscopic magnifier using a portable micromanipulator with a microscale attached. Each hemi-embryo (N = 60) and the remaining integer embryos (N = 15, used as controls) were immediately transferred individually to a recipient. The growth of the fetuses was evaluated ultrasonographically using a Esaote Aquila Pro vet ultrasound (Esaote SpA, Genoa, Italy). Transrectal measurements were performed at 60 and 90 days, recording femur length (only at 60 days), biparietal distance, and abdominal and umbilical cord diameter. At birth, animals were weighed, measured for length and height, and the circumferences of the skull, thorax, abdomen, and metatarsus were recorded. Data were analysed by the t-test implemented in InfoStat Version 1.1 software (https://www.infostat.com.ar/). Table 1 shows the average and standard deviation of all measurements performed. No significant differences (P < 0.05) were observed between hemi-embryos and control at any stage, except for biparietal diameter on Day 90 of gestation, which was not reflected in skull circumference at birth. In conclusion, individually transferred hemi-embryos generated 60-day fetuses with almost no differences in growth and development compared with the control embryos. The compensation phenomenon should have occurred before Day 60 of gestation. Table 1.Mean and standard deviation of all measurements performed on hemi-embryos and control embryos at 60 and 90 days and at birth

scholarly journals Evaluation of biparietal diameter and femur length by ultrasonography method, its correlation with fetal gestational age a comparative study in Chhattisgarh state of India

2021 ◽  
Vol 8 (4) ◽  
pp. 305-309
Author(s):  
Rahule A S ◽  
M L Ganware ◽  
Sidiqui Abdul Rafique ◽  
Goyal Meena ◽  
Netam S B S ◽  
...  
Keyword(s):  
Standard Deviation ◽  
Comparative Study ◽  
Gestational Age ◽  
Standard Error ◽  
Femur Length ◽  
Pregnant Females ◽  
Biparietal Diameter ◽  
Highly Correlated

There few studies which tried to corelate biparietal diameter and gestational age in which an increase in biparietal diameter with the increase in gestational age was found but there was a discrepancy between 17 week of pregnancy and term and which is approximately 3 weeks. We conducted the present study to compare the gestational age and femur length and biparietal diameter in the population of Chhattisgarh state of India.A total of 380 pregnant females were enrolled for the study. They were between the 20 weeks to 38 weeks of gestation with their age ranging from 18-35 years.In the present study we observed a total of 158 cases in 2 trimester i.e. ranging from 20 to 27 weeks and 220 cases were in 3 trimester of pregnancy. Mean of BPD and FL observed was 73.1 and 56.2 respectively. The standard deviation and standard error of mean for biparietal diameter and femur length were 12.8, 11.6 and 0.63, 0.57 respectively.All parameters of present study are found to be highly correlated with gestational age. (r=, 0.987, 0.980 for, FL and BPD respectively).

The Preservation of Specialization by Dispersed Steer Thyroidal Cells in Long-Term Culture

1971 ◽  
Vol 9 (1) ◽  
pp. 49-69
Author(s):  
E. SIEGEL
Keyword(s):  
Standard Deviation ◽  
Trichloroacetic Acid ◽  
Doubling Time ◽  
Ultrastructural Level ◽  
Thyroid Stimulating Hormone ◽  
Linear Variation ◽  
Thyroid Cells ◽  
Long Acting ◽  
Number Of Cells

Serially propagated cells derived from the steer thyroid gland preserve several specialized characteristics, some demonstrable for as long as 8 months (over 15 passes). For about 5 passes (3 months), follicular-like arrays of cells develop. Thyroid-stimulating hormone (TSH) or thyrotropin (1 and 10 mu./ml) induces the formation of supernumerary nucleoli, and promotes at the ultrastructural level the prompt (within 10 min) appearance of microvilli, pseudopodia, and intracytoplasmic droplets. These cells have a mean plating efficiency (PE) of 16.5 ± 4.5% (standard deviation) and with 3x104 cells as the standard inoculum, a mean doubling time (Td) of 43.7 ± 1.2 h. The linear variation of Td with the number of cells seeded probably signifies strong cell-cell interactions. TSH (1-5o mu./ml) influences both PE and Td, with 1 mu./ml producing the maximum stimulation. TSH (0.001-100 mu./ml) also induces the discharge of incorporated radio-iodide from the cultured thyroid cells, achieving a peak by 2-4 h, titres of 0.1-1 mu./ml being most potent. Long-acting thyroid stimulator (LATS) (0.2 u./ml) likewise effects release of 131I into growth medium (1 experiment), but over a more protracted period. After labelling with [3H]leucine, radioautographs demonstrate that the synthesis of proteins is stimulated by exposure to TSH (1 mu./ml). Precipitation with trichloroacetic acid indicates that these cells persist in synthesizing 131I-tagged iodoproteins, an activity optimally stimulated by 1 mu./ml TSH and marked by 3 h. Hence, such thyroidal cell lines afford a useful model for studying differentiation and hormonal effects.

scholarly journals Placental thickness: an important parameter in determining gestational age and fetal growth during ANC scan

2018 ◽  
Vol 7 (11) ◽  
pp. 4619
Author(s):  
Dipali Kadam ◽  
Saurabh Patil ◽  
Meenal Jain
Keyword(s):  
Gestational Age ◽  
Abdominal Circumference ◽  
P Value ◽  
Third Trimester ◽  
Cross Sectional ◽  
Femur Length ◽  
Biparietal Diameter ◽  
Antenatal Sonography ◽  
Umbilical Cord Insertion ◽  
Placental Thickness

Background: Gestational Age (GA) is one of the most imperative parameters required for proper management in pregnancy. Routinely GA is estimated by sonography utilising Biparietal Diameter (BPD), Femur Length (FL), Abdominal Circumference (AC) and Head Circumference (HC). In any case, these parameters have some limitations. Hence, there is need to find other parameters that may complement the established fetal biometric parameters in predicting GA. The objective of the present study was to assess placental thickness in second and third trimester pregnancies and its relationship with fetal gestational age and its role in detecting LBW and IUGRMethods: A cross sectional prospective study was carried out in three hundred pregnant women between 13 to 40 weeks of gestation, who came for routine antenatal sonography. Placental thickness was measured along with routine parameters. Placental thickness was measured at the level of umbilical cord insertion by two-dimensional ultrasonography.Results: Correlation between the GA by LMP and Placental thickness by ultrasound was done by using Karl Pearson's Correlation(r). The values were expressed as mean + standard deviation. Correlation between placental thickness and gestational age was statistically significant as p value is <0.01. Placental thickness measured in millimetres increases with gestational age in second and third trimester.Conclusions: The correlation between the placental thickness and gestational age was linear and direct. Therefore, Placental thickness is used as a predictor for estimation of gestational age of the fetus in cases where LMP is not known and in detecting developing IUGR and low birth weight.

Use of the Carbon Dioxide Laser for Acoustic Tumor Surgery

Neurosurgery ◽  
1983 ◽  
Vol 12 (3) ◽  
pp. 286-290 ◽  
Author(s):  
John H. Robertson ◽  
Craig W. Clark ◽  
James T. Robertson ◽  
Gale L. Gardner ◽  
Coyle M. Shea
Keyword(s):  
Carbon Dioxide ◽  
Surgical Technique ◽  
Carbon Dioxide Laser ◽  
Tumor Surgery ◽  
Operating Microscope ◽  
Intracranial Surgery ◽  
Technical Modifications ◽  
Acoustic Tumor ◽  
Neurological Surgery ◽  
And Control

Abstract The carbon dioxide laser has been used clinically in both otorhinolaryngology and neurological surgery for approximately 10 years. Only recently, however, have technical modifications allowed its use with the operating microscope, providing the increased precision and control necessary for intracranial surgery. This paper reports the authors' experience with the carbon dioxide laser in the removal of acoustic tumors, details the surgical technique involved, and describes the advantages that may accompany the use of the laser in the removal of these difficult lesions.

scholarly journals PENGARUH MODEL PEMBELAJARAN KOOPERATIF TIPE TEAMS GAMES AND TOURNAMENT (TGT) TERHADAP HASIL BELAJAR DASARLISTRIK DAN ELEKTRONIKA

2021 ◽  
Vol 1 (2) ◽  
pp. 123
Author(s):  
Dyan Novi Rezki Situmorang ◽  
Wanapri Pangaribuan
Keyword(s):  
Standard Deviation ◽  
Cooperative Learning ◽  
Learning Outcomes ◽  
Learning Model ◽  
Average Score ◽  
Homogeneous Distribution ◽  
Electrical Measurements ◽  
Model Type ◽  
Team Game ◽  
And Control

AbstrakJenis penelitian ini adalah penelitian quasi eksperimen. Populasi dalam penelitian ini adalah seluruh siswa kelas X semester genap SMK Swasta Imelda Medan Tahun Ajaran 2019/2020. Pengambilan sampel dilakukan dengan mengambil 2 kelas, yaitu kelas X TITL 1 sebagai kelas eksperimen dan kelas X TITL 2 sebagai kelas kontrol, yang masing-masing kelas berjumlah 28 orang siswa. Instrument yang digunakan untuk mengetahui hasil belajar siswa adalah tes hasil belajar dalam bentuk pilihan berganda dengan jumlah soal 30 butir. Hasil pengujian pretest sebelum diberi perlakuan yang berbeda, yaitu skor rata-rata di kelas eksperimen  (47) dengan standar deviasi 8,50 dan skor rata-rata di kelas kontrol (44,45) dengan standar deviasi 7,75. Pada pengujian data pretest kedua kelas diperoleh bahwa data kedua kelas berdistribusi normal dan homogen yang berarti memiliki kemampuan awal yang sama. Kemudian diberi perlakuan yaitu kelas eksperimen diajarkan dengan pembelajaran menggunakan model kooperatif tipe Team Game Tournament (TGT) dan kelas kontrol diajar dengan pembelajaran menggunakan model ekspositori. Setelah pembelajaran selesai diberikan posttest, diperoleh nilai posttest dengan hasil rata-rata kelas eksperimen (88,78) dengan standar deviasi 4,06 dan kelas kontrol (81,55) dengan standar deviasi 4,17. Dari hasil pengolahan data posttest diperoleh bahwa thitung=  6,40 dan ttabel = 1,67. Sehingga thitung > ttabel.Sehingga Ha diterima yaitu Model Pembelajaran Kooperatif Tipe Team Game Tournament (TGT)memberikan hasil belajar pada pelajaran dasar dan pengukuran listrik yang lebih tinggi daripada pembelajaran menggunakan model ekspositori pada siswa kelas X TITL SMK Swasta Imelda Medan.Kata Kunci: Pembelajaran Tipe Team Game Tournament, Model Pembelajaran Kooperatif AbstractEnglish translation. This type of research is quasi experimental research. The population in this study is all students of class X even semester of Imelda Medan Private Vocational School Year 2019/2020. Sampling is done by taking 2 classes, namely class X TITL 1 as an experimental class and class X TITL 2 as a control class, each of which totals 28 students. The instrument used to find out student learning outcomes is a test of learning results in the form of multiple choices with the number of questions 30 points. Pretest test results before being given different treatments, namely the average score in the experimental class (47) with a standard deviation of 8.50 and the average score in the control class (44.45) with a standard deviation of 7.75. In the pretest data test both classes it was obtained that the data of both classes are normal and homogeneous distribution which means it has the same initial capabilities. Then given the treatment that experimental classes are taught by learning using a cooperative model type Team Game Tournament (TGT) and control classes are taught by learning using an expository model. After the learning was completed, posttest scores were obtained with the average results of experimental classes (88.78) with a standard deviation of 4.06 and control classes (81.55) with a standard deviation of 4.17. From the results of posttest data processing obtained that thitung = 6.40 and ttabel = 1.67. So thitung > ttabel. So ha accepted, namely the Cooperative Learning Model Type Team Game Tournament (TGT) provides learning outcomes on basic lessons and higher electrical measurements than learning using the expository model in students of class X TITL SMK Swasta Imelda Medan.  Keywords: Team Game Tournament Type Learning, Cooperative Learning Model

scholarly journals FEATURES OF BRASSICA RAPA'S GROWTH AND DEVELOPMENT, DEPENDING ON THE TERRITORY OF SOWING THE SEED

2019 ◽  
pp. 183-194
Author(s):  
Serhii Vdovenko
Keyword(s):  
Seed Germination ◽  
Soil Temperature ◽  
Growth And Development ◽  
Total Yield ◽  
Growing Season ◽  
Vegetation Period ◽  
Row Spacing ◽  
The Third ◽  
Golden Ball ◽  
And Control

In order to study the effect of the time of sowing seeds on the passage of morphogenesis and productivity of rapeseed, the scheme included experiments where the seeds were sown in I, II, III in April, I and II in May. The experiment used varieties of Golden Ball, Purpurleopop, Purpurov and Geisha, and control was the variant in which the seeds of the Golden Ball varieties were sown in the second decade of April with a row spacing of 45 cm. laboratory - to determine the total yield; statistical - to establish the reliability of the investigated factors. Seed germination analysis used in the experiment ranged from 78.9% to 81.1% on average. The highest indicator of seed germination was characterized by the Purpulepop variety, where the studied value was 83.2%, which was dominated by the seed germination of the control variant by 2.1%. In the early stages of sowing seeds, the period of seedling formation was shorter. Seeding of seeds in the first two decades of April on the surface of the soil was observed for 8 days. The short sprouting period was characterized by all varieties of turnips. As a result of sowing turnip seeds at a later date, namely in the third decade of April - the first and second decade of May and the setting of a higher soil temperature, the period of germination was extended and ranged from 8 to 10 days. By using the term of sowing seeds I-II decade of April, the short interphase period was characterized by Purpleupop and Purpurova varieties, where the specified period was only 22 days. The sowing of turnip seeds at a later date ensured the formation of an interphase period of "seedlings - leaf rosette" in 23-25 days except Purpulopop variety. The interphase period "leaf rosette - the beginning of rooting" and "the beginning of rooting - technical maturity" determined the benefits of early seeding, namely in the first decade of April on varieties Purpulopop and Purpurov.The short vegetation period was characterized by the Golden Ball plants for sowing seeds in the first and third decades of April and the first of May. During the specified seeding period the duration of the growing season was only 40 days. A longer vegetation period was characterized by plants of the Geisha variety for sowing seeds in the first two decades of April. As a result of the use of the researched elements of technology to grow turnips in open soil, the total yield varied from 11.0 t / ha to 24.0 t / ha. The most productive is the first sowing of rapeseed, where its value ranged from 20.6 t / ha to 22.7 t / ha. At the same time, when sowing seeds in the first decade of April, the yield of turnip varieties Purpulepop was the highest and was 22.7 t / ha, or the increase was 2.4 t / ha.

scholarly journals Effectiveness of the Group Play Therapy on the Insecure Attachment and Social Skills of Orphans in Ahvaz City

2016 ◽  
Vol 9 (9) ◽  
pp. 42
Author(s):  
Bahareh Mousavi ◽  
Sahar Safarzadeh
Keyword(s):  
Social Skills ◽  
Standard Deviation ◽  
Treatment Group ◽  
Play Therapy ◽  
Insecure Attachment ◽  
Control Group ◽  
Group Play Therapy ◽  
Group Play ◽  
And Control

<p class="apa">This study aimed to determine the effectiveness of the group play therapy on the insecure attachment and social skills of orphans in Ahvaz city. Statistical population included all orphans in Ahvaz city, of whom 30 students were selected whose scores in insecure attachment and in social skills were one standard deviation higher and one standard deviation lower than the mean, respectively and they were randomly divided into two treatment (15 persons) and control (15 persons) groups. The research tools included Randolph Attachment Disorder Questionnaire (2000) (RADQ) and Social Skills Rating System (SSRS) questionnaire (Gresham and Elliot, 1990). This is an experimental study with pretest, posttest, and follow-up by the control group. Firstly, pretest was implemented for both groups, and then experimental intervention (play therapy) was carried out for the treatment group during 10 sessions. After the therapeutic program, the posttest and two months later follow-up were implemented. The results obtained using the statistical method of multivariate covariance analysis showed that group play therapy reduces the insecure attachment and increases the social skills at P &lt; 0.001 during the stages of posttest and follow-up in the treatment group compared to the control group. Results also indicated that there is a significant difference between posttest and follow-up of the treatment and control group in terms of the components of social skills (collaboration, assertiveness, and self-control).</p>

scholarly journals Effect of salicylic acid on pre-transplant acclimatization of native tomato populations

2018 ◽  
Vol 36 (4) ◽  
pp. 480-485
Author(s):  
Wilberth A Poot-Poot ◽  
Rafael Delgado-Martínez ◽  
Sergio Castro-Nava ◽  
Ma Teresa Segura-Martínez ◽  
Alejandro Carreón-Pérez ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Growth And Development ◽  
Growth Parameters ◽  
Field Capacity ◽  
Dry Weight ◽  
Growth Conditions ◽  
Peat Moss ◽  
Hypocotyl Length ◽  
Cell Processes ◽  
Recorded Data

ABSTRACT The aim of this study was to evaluate the effect of salicylic acid (SA) application on pre-transplant acclimatization of native populations of tomato. SA is a growth regulator that modifies plant growth and development by inducing changes in cell processes, physiology and morphology. Five populations of native tomato were sown in polystyrene trays. Peat moss was used as substrate and plants were maintained at field capacity continuously. After emergence, seedlings were applied during three weeks with different concentration of SA (0.0, 0.01, 0.1, 0.5 and 1.0 μM). A completely random experimental design was used with five replications per treatment. The growth parameters evaluated were height, stem diameter, number of leaves, hypocotyl length, shoot fresh and dry weight (leaves and stem), and root length and fresh and dry weight. An analysis of variance was carried out, and means were compared with the Tukey test (5%) using SAS statistical software. The recorded data show that pre-transplant seedlings of each of the evaluated populations responded significantly (P=0.01) to the SA treatments. Also, the comparison of means of each of the factors under study showed positive changes. With the concentrations of 0.5 and 0.1 μM SA, higher values of the studied variables were obtained than with the concentrations 0.01 and 1.0 μM SA. The native tomato populations sprayed with SA at concentrations of 0.5 and 0.1 μM responded positively in terms of seedling growth and development. Based on these findings, SA treatments can help acclimatize and present better growth conditions to the seedlings before being transplanted.

scholarly journals Correlation models for monitoring fetal growth

2020 ◽  
Vol 29 (10) ◽  
pp. 2795-2813 ◽  
Author(s):  
Yuan Feng ◽  
Luo Xiao ◽  
Cai Li ◽  
Stephanie T Chen ◽  
Eric O Ohuma
Keyword(s):  
Fetal Growth ◽  
Abdominal Circumference ◽  
Fetal Head ◽  
Femur Length ◽  
Second Stage ◽  
Correlation Models ◽  
Biparietal Diameter ◽  
Z Scores ◽  
Using Data ◽  
Shiny Application

Ultrasound growth measurements are monitored to evaluate if a fetus is growing normally compared with a defined standard chart at a specified gestational age. Using data from the Fetal Growth Longitudinal Study of the INTERGROWTH-21st project, we have modelled the longitudinal dependence of fetal head circumference, biparietal diameter, occipito-frontal diameter, abdominal circumference, and femur length using a two-stage approach. The first stage involved finding a suitable transformation of the raw fetal measurements (as the marginal distributions of ultrasound measurements were non-normal) to standardized deviations (Z-scores). In the second stage, a correlation model for a Gaussian process is fitted, yielding a correlation for any pair of observations made between 14 and 40 weeks. The correlation structure of the fetal Z-score can be used to assess whether the growth, for example, between successive measurements is satisfactory. The paper is accompanied by a Shiny application, see https://lxiao5.shinyapps.io/shinycalculator/ .

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