118 Influence of sire fertility status on conceptus-induced transcriptomic response of the bovine endometrium

Farmers in dry regions of India have a high tendency for leaving their land fallow. To decipher this phenomenon, the study was carried out in the Tumkur district of the central dry zone of Karnataka. Results from the study revealed a positive relationship between the size of land holdings and land fallow. The major reason stated by farmers for leaving their fallow land was the scarcity of rainfall or irrigation, and poor land fertility status. The Tobit regression model was fitted to study the determinants of the decision of farmers to keep the arable land fallow, where, dependent variable considered was the share of fallow land in the total size of landholding of farm households. It was found that the availability of water for irrigation and family labour has a negative relationship with fallow land. Other factors determining the extent of fallow land were the distance of land from residences, poor land fertility status and availability of credit.

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Charged Residues Flanking the Transmembrane Domain of Two Related Toxin–Antitoxin System Toxins Affect Host Response

Toxins ◽

10.3390/toxins13050329 ◽

2021 ◽

Vol 13 (5) ◽

pp. 329

Author(s):

Andrew Holmes ◽

Jessie Sadlon ◽

Keith Weaver

Keyword(s):

Amino Acid ◽

Enterococcus Faecalis ◽

Host Response ◽

Cytoplasmic Membrane ◽

Transmembrane Domain ◽

The Other ◽

Type I ◽

Transcriptomic Response ◽

Specific Amino Acid ◽

Transporter Protein

A majority of toxins produced by type I toxin–antitoxin (TA-1) systems are small membrane-localized proteins that were initially proposed to kill cells by forming non-specific pores in the cytoplasmic membrane. The examination of the effects of numerous TA-1 systems indicates that this is not the mechanism of action of many of these proteins. Enterococcus faecalis produces two toxins of the Fst/Ldr family, one encoded on pheromone-responsive conjugative plasmids (FstpAD1) and the other on the chromosome, FstEF0409. Previous results demonstrated that overexpression of the toxins produced a differential transcriptomic response in E. faecalis cells. In this report, we identify the specific amino acid differences between the two toxins responsible for the differential response of a gene highly induced by FstpAD1 but not FstEF0409. In addition, we demonstrate that a transporter protein that is genetically linked to the chromosomal version of the TA-1 system functions to limit the toxicity of the protein.

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Current Fertility Status Does Not Predict Sociosexual Attitudes and Desires in Normally Ovulating Women

Evolutionary Psychology ◽

10.1177/1474704920976318 ◽

2021 ◽

Vol 19 (1) ◽

pp. 147470492097631 ◽

Cited By ~ 1

Author(s):

Andrew G. Thomas ◽

Stephanie L. Armstrong ◽

Steve Stewart-Williams ◽

Benedict C. Jones

Keyword(s):

Relationship Status ◽

Counting Method ◽

Ongoing Debate ◽

Fertility Status ◽

Current Fertility

Previous research has found that women at peak fertility show greater interest in extra-pair sex. However, recent replications have failed to detect this effect. In this study, we add to this ongoing debate by testing whether sociosexuality (the willingness to have sex in the absence of commitment) is higher in women who are at peak fertility. A sample of normally ovulating women ( N = 773) completed a measure of sociosexuality and had their current fertility status estimated using the backward counting method. Contrary to our hypothesis, current fertility was unrelated to sociosexual attitudes and desires, even when relationship status was included as a moderator. These findings raise further doubts about the association between fertility and desire for extra-pair sex.

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Environmental Conditions Modulate the Transcriptomic Response of Both Caulobacter crescentus Morphotypes to Cu Stress

Microorganisms ◽

10.3390/microorganisms9061116 ◽

2021 ◽

Vol 9 (6) ◽

pp. 1116

Author(s):

Laurens Maertens ◽

Pauline Cherry ◽

Françoise Tilquin ◽

Rob Van Houdt ◽

Jean-Yves Matroule

Keyword(s):

Oxidative Stress ◽

Stress Response ◽

Stress Responses ◽

Caulobacter Crescentus ◽

Oxidative Stress Response ◽

Transport Systems ◽

Transcriptomic Response ◽

Swarmer Cell ◽

Cu Stress ◽

Cellular Environment

Bacteria encounter elevated copper (Cu) concentrations in multiple environments, varying from mining wastes to antimicrobial applications of copper. As the role of the environment in the bacterial response to Cu ion exposure remains elusive, we used a tagRNA-seq approach to elucidate the disparate responses of two morphotypes of Caulobacter crescentus NA1000 to moderate Cu stress in a complex rich (PYE) medium and a defined poor (M2G) medium. The transcriptome was more responsive in M2G, where we observed an extensive oxidative stress response and reconfiguration of the proteome, as well as the induction of metal resistance clusters. In PYE, little evidence was found for an oxidative stress response, but several transport systems were differentially expressed, and an increased need for histidine was apparent. These results show that the Cu stress response is strongly dependent on the cellular environment. In addition, induction of the extracytoplasmic function sigma factor SigF and its regulon was shared by the Cu stress responses in both media, and its central role was confirmed by the phenotypic screening of a sigF::Tn5 mutant. In both media, stalked cells were more responsive to Cu stress than swarmer cells, and a stronger basal expression of several cell protection systems was noted, indicating that the swarmer cell is inherently more Cu resistant. Our approach also allowed for detecting several new transcription start sites, putatively indicating small regulatory RNAs, and additional levels of Cu-responsive regulation.

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Transcriptomic response of Anopheles gambiae sensu stricto mosquito larvae to Curry tree (Murraya koenigii) phytochemicals

Parasites & Vectors ◽

10.1186/s13071-020-04505-4 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Clarence M. Mang’era ◽

Fathiya M. Khamis ◽

Erick O. Awuoche ◽

Ahmed Hassanali ◽

Fidelis Levi Odhiambo Ombura ◽

...

Keyword(s):

Immune Response ◽

Anopheles Gambiae ◽

Growth And Development ◽

Leaf Extract ◽

Expression Profiles ◽

Sensu Stricto ◽

Mosquito Larvae ◽

Insect Vector ◽

Murraya Koenigii ◽

Transcriptomic Response

Abstract Background Insect growth regulators (IGRs) can control insect vector populations by disrupting growth and development in juvenile stages of the vectors. We previously identified and described the curry tree (Murraya koenigii (L.) Spreng) phytochemical leaf extract composition (neplanocin A, 3-(1-naphthyl)-l-alanine, lumiflavine, terezine C, agelaspongin and murrayazolinol), which disrupted growth and development in Anopheles gambiae sensu stricto mosquito larvae by inducing morphogenetic abnormalities, reducing locomotion and delaying pupation in the mosquito. Here, we attempted to establish the transcriptional process in the larvae that underpins these phenotypes in the mosquito. Methods We first exposed third-fourth instar larvae of the mosquito to the leaf extract and consequently the inherent phytochemicals (and corresponding non-exposed controls) in two independent biological replicates. We collected the larvae for our experiments sampled 24 h before peak pupation, which was 7 and 18 days post-exposure for controls and exposed larvae, respectively. The differences in duration to peak pupation were due to extract-induced growth delay in the larvae. The two study groups (exposed vs control) were consequently not age-matched. We then sequentially (i) isolated RNA (whole larvae) from each replicate treatment, (ii) sequenced the RNA on Illumina HiSeq platform, (iii) performed differential bioinformatics analyses between libraries (exposed vs control) and (iv) independently validated the transcriptome expression profiles through RT-qPCR. Results Our analyses revealed significant induction of transcripts predominantly associated with hard cuticular proteins, juvenile hormone esterases, immunity and detoxification in the larvae samples exposed to the extract relative to the non-exposed control samples. Our analysis also revealed alteration of pathways functionally associated with putrescine metabolism and structural constituents of the cuticle in the extract-exposed larvae relative to the non-exposed control, putatively linked to the exoskeleton and immune response in the larvae. The extract-exposed larvae also appeared to have suppressed pathways functionally associated with molting, cell division and growth in the larvae. However, given the age mismatch between the extract-exposed and non-exposed larvae, we can attribute the modulation of innate immune, detoxification, cuticular and associated transcripts and pathways we observed to effects of age differences among the larvae samples (exposed vs control) and to exposures of the larvae to the extract. Conclusions The exposure treatment appears to disrupt cuticular development, immune response and oxidative stress pathways in Anopheles gambiae s.s larvae. These pathways can potentially be targeted in development of more efficacious curry tree phytochemical-based IGRs against An. gambiae s.s mosquito larvae.

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Nonlinear transcriptomic response to dietary fat intake in the small intestine of C57BL/6J mice

BMC Genomics ◽

10.1186/s12864-016-2424-9 ◽

2016 ◽

Vol 17 (1) ◽

Cited By ~ 5

Author(s):

Tenzin Nyima ◽

Michael Müller ◽

Guido J. E. J. Hooiveld ◽

Melissa J. Morine ◽

Marco Scotti

Keyword(s):

Small Intestine ◽

Dietary Fat ◽

Fat Intake ◽

Transcriptomic Response ◽

Dietary Fat Intake

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Survival and Transcriptomic response of Salmonella enterica on fresh-cut fruits

International Journal of Food Microbiology ◽

10.1016/j.ijfoodmicro.2021.109201 ◽

2021 ◽

pp. 109201

Author(s):

Yingshu He ◽

Ruixi Chen ◽

Yan Qi ◽

Joelle K. Salazar ◽

Shimei Zhang ◽

...

Keyword(s):

Salmonella Enterica ◽

Transcriptomic Response ◽

Fresh Cut

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Dissecting the Role of Thyrotropin in the DNA Damage Response in Human Thyrocytes after 131I, γ Radiation and H2O2

The Journal of Clinical Endocrinology & Metabolism ◽

10.1210/clinem/dgz185 ◽

2019 ◽

Vol 105 (3) ◽

pp. 839-853

Author(s):

Aglaia Kyrilli ◽

David Gacquer ◽

Vincent Detours ◽

Anne Lefort ◽

Frédéric Libert ◽

...

Keyword(s):

Gene Expression ◽

Cell Cycle ◽

Dna Damage ◽

Dna Damage Response ◽

Iodide Uptake ◽

Transcriptomic Response ◽

Uptake Inhibition ◽

Γ Radiation ◽

Damage Response

Abstract Background The early molecular events in human thyrocytes after 131I exposure have not yet been unravelled. Therefore, we investigated the role of TSH in the 131I-induced DNA damage response and gene expression in primary cultured human thyrocytes. Methods Following exposure of thyrocytes, in the presence or absence of TSH, to 131I (β radiation), γ radiation (3 Gy), and hydrogen peroxide (H2O2), we assessed DNA damage, proliferation, and cell-cycle status. We conducted RNA sequencing to profile gene expression after each type of exposure and evaluated the influence of TSH on each transcriptomic response. Results Overall, the thyrocyte responses following exposure to β or γ radiation and to H2O2 were similar. However, TSH increased 131I-induced DNA damage, an effect partially diminished after iodide uptake inhibition. Specifically, TSH increased the number of DNA double-strand breaks in nonexposed thyrocytes and thus predisposed them to greater damage following 131I exposure. This effect most likely occurred via Gα q cascade and a rise in intracellular reactive oxygen species (ROS) levels. β and γ radiation prolonged thyroid cell-cycle arrest to a similar extent without sign of apoptosis. The gene expression profiles of thyrocytes exposed to β/γ radiation or H2O2 were overlapping. Modulations in genes involved in inflammatory response, apoptosis, and proliferation were observed. TSH increased the number and intensity of modulation of differentially expressed genes after 131I exposure. Conclusions TSH specifically increased 131I-induced DNA damage probably via a rise in ROS levels and produced a more prominent transcriptomic response after exposure to 131I.

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Copper Availability Influences the Transcriptomic Response of Candida albicans to Fluconazole Stress

G3 Genes|Genome|Genetics ◽

10.1093/g3journal/jkab065 ◽

2021 ◽

Vol 11 (4) ◽

Author(s):

Elizabeth W Hunsaker ◽

Chen-Hsin Albert Yu ◽

Katherine J Franz

Keyword(s):

Candida Albicans ◽

Time Course ◽

Transcriptional Response ◽

Metal Homeostasis ◽

Metal Availability ◽

Drug Induced ◽

Transcriptomic Response ◽

Opportunistic Fungal Pathogen ◽

Whole Transcriptome Analysis ◽

Whole Transcriptome

Abstract The ability of pathogens to maintain homeostatic levels of essential biometals is known to be important for survival and virulence in a host, which itself regulates metal availability as part of its response to infection. Given this importance of metal homeostasis, we sought to address how the availability of copper in particular impacts the response of the opportunistic fungal pathogen Candida albicans to treatment with the antifungal drug fluconazole. The present study reports whole transcriptome analysis via time-course RNA-seq of C. albicans cells exposed to fluconazole with and without 10 µM supplemental CuSO4 added to the growth medium. The results show widespread impacts of small changes in Cu availability on the transcriptional response of C. albicans to fluconazole. Of the 2359 genes that were differentially expressed under conditions of cotreatment, 50% were found to be driven uniquely by exposure to both Cu and fluconazole. The breadth of metabolic processes that were affected by cotreatment illuminates a fundamental intersectionality between Cu metabolism and fungal response to drug stress. More generally, these results show that seemingly minor fluctuations in Cu availability are sufficient to shift cells’ transcriptional response to drug stress. Ultimately, the findings may inform the development of new strategies that capitalize on drug-induced vulnerabilities in metal homeostasis pathways.

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Acute electronic vapour product whole aerosol exposure of 3D human bronchial tissue results in minimal cellular and transcriptomic responses when compared to cigarette smoke

Toxicology Research and Application ◽

10.1177/2397847320988496 ◽

2021 ◽

Vol 5 ◽

pp. 239784732098849

Author(s):

Gary Phillips ◽

Lukasz Czekala ◽

Holger P Behrsing ◽

Khalid Amin ◽

Jessica Budde ◽

...

Keyword(s):

Cigarette Smoke ◽

Recovery Time ◽

Beat Frequency ◽

Transcriptomic Response ◽

Potential Harm ◽

Aerosol Exposure ◽

Cell Culture Systems ◽

Computational Sciences ◽

Potential Risks ◽

Reduced Toxicity

The use of electronic vapour products (EVPs) continues to increase worldwide and with advances in cell culture systems, molecular biology and the computational sciences there is also accumulating evidence of their potential reduced toxicity and reduced potential harm when compared to cigarette smoke. To further understand the potential risks and health effects associated with exposure to EVP aerosols we have assessed the cellular and transcriptomic response from a commercially available lung tissue culture system (MucilAirTM) following a single sub-cytotoxic exposure to cigarette smoke and the equivalent nicotine delivered dose of EVP aerosol. The transcriptomic, cellular (cilia beat frequency (CBF) and percent active area (%AA), trans epithelial electrical resistance (TEER), histology) and cytokine release were assessed at 4- and 48- hours following recovery from air, EVP aerosol (8.4% V/V: mybluTM blueberry flavour, 2.4% nicotine) and 3R4F smoke (3.5% V/V: exposure). No pathological changes were observed at either recovery time point from any exposure. Air and EVP aerosol exposure had no effect on CBF, %AA nor TEER at 48 hours. Exposure to cigarette smoke resulted in a decrease in TEER, an increase in CBF and the release of proinflammatory cytokines at both recovery time points. Although the number of significantly expressed genes was minimal following exposure to EVP aerosol, exposure to 3R4F smoke resulted in a significant upregulation of several disease relevant pathways. These data provide evidence that following an acute exposure to EVP aerosol there is significantly less damage to lung cells in culture than the equivalent, nicotine based, dose of cigarette smoke.

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