Screening of some Leguminosae seeds for nematicidal activity

Root-knot nematodes, Meloidogyne spp., are the major nematode pests of economic crops worldwide. Use of conventional nematicides has been one of the most effective methods to reduce their populations. However, the ban on many effective nematicides in recent past has necessitated the search for alternative sources of effective and ecofriendly chemicals for nematode control. The nematicidal potential of fifteen Leguminosae seeds (wild and cultivated) was assessed, in vitro, against juveniles of Meloidogyne incognita (Kofoid & White) Chitwood. Each standard aqueous seed extract, 25g/100ml (w/v), was evaluated at dilutions, 1:10, 1:20, and 1:40 at 27+1�C for 24 hours. The majority of seed extracts caused high mortality rates, especially in 1:10 concentration. Trigonella foenum graceum, Sesbania sesban, Albizzia lebbak, Cassia fistula and Pongamia glabra were the most efficacious (above 90% mortality). S. sesban and P. glabra were significantly effective even in the lowest concentration (1:40).

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EVALUATION OF IN VITRO ANTI-INFLAMMATORY AND ANTIMICROBIAL ACTIVITY OF AQUEOUS AND METHANOLIC SEED EXTRACTS OF CITRULLUS LANATUS

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i5.16708 ◽

2017 ◽

Vol 9 (5) ◽

pp. 29

Author(s):

Iswariya S. ◽

Uma T. S.

Keyword(s):

Antimicrobial Activity ◽

Bacillus Subtilis ◽

Aqueous Extract ◽

Seed Extract ◽

Inflammatory Activity ◽

Diffusion Method ◽

Klebsiella Pneumonia ◽

Anti Inflammatory ◽

Seed Extracts

Objective: The present study was designed to identify the bioactive phytochemicals and its antibacterial and in vitro anti-inflammatory potential of aqueous and methanolic seed extract of Citrullus lanatus.Methods: The phytochemical screening of both the aqueous and methanolic seed extract was carried out qualitatively to identify the major Phyto-constituents present in the extracts. The antimicrobial activity of the extracts was evaluated against six pathogenic bacterial strains by agar well diffusion method and the Minimum inhibitory concentration (MIC) was determined by broth dilution method. In vitro anti-inflammatory activity of C. lanatus seed extracts was evaluated by using human red blood cell (HRBC) membrane stabilization and inhibition of albumin denaturation method.Results: The results of the study indicated that both the extracts of the seed having antimicrobial activity, while the methanolic extract showed more significant activity against the tested organism than aqueous extract. Methanol extract had the lowest MIC of 1.562 mg/ml against Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aeruginosa and Bacillus subtilis, whereas in aqueous extract was highly sensitive to Bacillus subtilis, E. coli and Klebsiella pneumonia with MIC of 3.125 and 6.25 mg/ml, respectively. Methanolic extracts exerted comparative higher anti-inflammatory activity than aqueous extract.Conclusion: Present study provides a firm evidence to support that the synergistic effect of C. lanatus seed extracts having potent anti-inflammatory and antimicrobial property, which might serve as an effective drug for various microbial infections and inflammatory disorders.

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In vitro antioxidant and antidiarrheal activities of aqueous and n-hexane extracts of Cucurbita maxima seed in castor oil-induced diarrheal rats

10.53365/nrfhh/144728 ◽

2022 ◽

Author(s):

Habibu Tijjani ◽

Adamu Matinja ◽

Marwanatu Yahya ◽

Emmanuel Aondofa ◽

Akibu Sani

Keyword(s):

Vitamin C ◽

Aqueous Extract ◽

Castor Oil ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Seed Extract ◽

Hexane Extract ◽

Cucurbita Maxima ◽

Seed Extracts

Diarrhea is a common health complaint occurring with mild, temporary conditions to a potentially life-threatening condition. Cucurbita maxima (Cucurbitaceae) seed is reportedly used traditionally for the treatment of diarrheal and thus this study aimed to evaluate the in vitro antioxidants, total tannins, phenolics contents, and antidiarrheal potentials of n-hexane and aqueous C. maxima seed extracts in castor oil induced diarrheal rats. The n-hexane extract mainly contains oils while the aqueous extract was thick, brown solid. The aqueous and n-hexane seed extract of C. maxima expressed significant 2,2, diphenyl-1-picrythdrazyl (DPPH) scavenging activities at 6.25 – 1000 mg/mL with IC50 values of 104.01 mg/mL, 29.27 mg/mL and 26.78 mg/mL for n-hexane, aqueous and vitamin C respectively. The hydroxyl radical scavenging activities of the n-hexane and aqueous seed extract of C. maxima were significantly lower at higher concentrations compared with that of vitamin C. Furthermore, the total antioxidant capacities of n-hexane (22.08mg/mL) and aqueous seed extract (11.03 mg/mL) of C. maxima were found to be higher than that of vitamin C (134.46 mg/mL). The aqueous extracts (658.33±380.08 mg QE/g) total tannins were not significantly different from the n-hexane extract (468.33±102.55 mg QE/g) while the n-hexane extract contains significantly higher total phenolics (2.93±1.25 mg GAE/g) compared with the aqueous extract (0.19±0.04 mg GAE/g). Percentage inhibition of stooling was found to be 57±22%, 41±16%, and 46±11% for loperamide, n-hexane, and aqueous respectively. The study concludes that aqueous and n-hexane seed extracts of C. maxima (pumpkin) possess in vitro antioxidant activities and antidiarrheal properties.

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In Vitro, in Situ and in Vivo Studies on the Anticandidal Activity of Cassia fistula Seed Extract

Molecules ◽

10.3390/molecules17066997 ◽

2012 ◽

Vol 17 (6) ◽

pp. 6997-7009 ◽

Cited By ~ 8

Author(s):

Subramanion L. Jothy ◽

Zuraini Zakariah ◽

Yeng Chen ◽

Sreenivasan Sasidharan

Keyword(s):

Seed Extract ◽

In Vivo Studies ◽

Cassia Fistula ◽

Anticandidal Activity

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A study on in vitro antioxidant activity of aqueous seed extract of sesbania sesban (l) merr.

Kongunadu Research Journal ◽

10.26524/krj.2021.21 ◽

2021 ◽

Vol 8 (2) ◽

pp. 69-74

Author(s):

Kathiravan S ◽

Shwetha V Kalava

Keyword(s):

Antioxidant Activity ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Seed Extract ◽

Dependent Manner ◽

Sesbania Sesban ◽

Frap Assay ◽

Increase With Increase ◽

In Vitro Antioxidant Activity

The present study was done to investigate the in vitro antioxidant activity of aqueous extract of Sesbania sesban seeds. The assays such as DPPH, Chelation, ferrous ion, ABTS, Superoxide radical, hydroxyl radical assay, FRAP assay and total antioxidant activity were done to assess the antioxidant potential of the seed extract. The extract was tested at a concentration range of 100 – 500 μg/ml for all the assays and the values were compared with a standard. The results obtained showed that the radical scavenging activity was in a dose dependent manner and found to increase with increase in concentration of the extract. The IC50 value was calculated for the assays and tabulated for inference. Different assays revealed different levels of radical scavenging potential of the extract and exhibited as a better antioxidant source for therapeutic applications.

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In-vitro antibacterial activity of the seed extract of three-member Artocarpus towards Methicillin-Resistant Staphylococcus aureus (MRSA)

Jurnal Teknologi Laboratorium ◽

10.29238/teknolabjournal.v9i2.237 ◽

2020 ◽

Vol 9 (2) ◽

pp. 128-135

Author(s):

Muhammad Evy Prastiyanto ◽

Inas Hasna Azizah ◽

Hafizha Dara Haqi ◽

Bagus Dwi Yulianto ◽

Aulia Bella Agmala ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Methicillin Resistant Staphylococcus Aureus ◽

Seed Extract ◽

Methicillin Resistant ◽

Biological Source ◽

Agar Well Diffusion Assay ◽

Diffusion Assay ◽

Seed Extracts

Methicillin-Resistant Staphylococcus aureus (MRSA) infections have created a critical need for the development of natural antibacterials from a biological source. This research aimed to investigate the antibacterial activity of the seed extract of three-member Artocarpus (Artocarpus heterophyllus, A. champeden, and A. camansi) against MRSA which are the most prevalent causes of infections in patients. Crude seed extracts of three-member Artocarpus were evaluated for their antibacterial activity against MRSA. The antibacterial activity against MRSA of the three extracts was assayed in vitro by the agar well diffusion assay and agar microdilution method and minimum bactericidal concentration. The antibacterial activity, calculated as a zone of inhibition and MIC, MBC values. The Crude seed extracts of three-member Artocarpus showed antibacterial activity against the MRSA in the agar well diffusion assay (1.5-9 mm inhibition diameter). The MIC value of extract showed at 15.62 mg/mL and the MBC value of seed extract of A. heterophyllus at 62.5 mg/mL, A. champeden at 31.25 mg/mL, A. camansi at 250 mg/mL. All seed extracts have the potential to be developed as antibacterial agents, particularly against MRSA strain. Studies on the antibacterial activity against MRSA can provide new information about the benefits seed of members of Artocarpus as a source of natural antibacterial.

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Anthelmintic effects of pumpkin (Cucurbita moschata) seed extract on Ascaris suum in vitro

Biofarmasi Journal of Natural Product Biochemistry ◽

10.13057/biofar/f100101 ◽

2017 ◽

Vol 10 (1) ◽

pp. 1-6

Author(s):

SHITA GANESTYA ◽

SUTARMIADJI DJUMARGA ◽

CR. SITI UTARI

Keyword(s):

Ascaris Suum ◽

Sampling Technique ◽

Seed Extract ◽

Cucurbita Moschata ◽

Spearman Correlation ◽

Pumpkin Seed ◽

Death Time ◽

Significant Difference ◽

Seed Extracts

Ganestya S, Djumarga S, Utari CRS. 2012. Anthelmintic effects of pumpkin (Cucurbita moschata) seed extract on Ascaris suum in vitro. Biofarmasi 10: 1-6. The aim of this research was to determine the effect of pumpkin seed extract towards the Lethal Death Time of Ascaris suum Goeze in vitro. The type of this research was an quasi-experiment using a post-test only controlled group design. The subject of this research was the actively motile adult Ascaris suum Goeze. A purposive sampling technique was used to collect the samples by considering the length of each worm without noticing the sex of worm. The subjects were divided into four groups, each group consisted of 10 worms, with 5 times replication. Physiological saline solution and pyrantel pamoate 0.236% were used as the negative and positive control groups, respectively, while the pumpkin seed extracts used for the treatment groups were at the concentrations of 54.5% and 70.5%. The observation and checking towards the dead worm were performed once an hour. The data obtained were analyzed by using a Kruskal-Wallis test, continued with Mann-Whitney and Spearman Correlation tests. The Lethal Death Time of A. suum by pumpkin seed extracts at a concentration of 54.5% was 11 hour 48 minutes, while at a concentration of 70.5%, the Lethal Death Time was 7 hours 48 minutes. The result of Kruskal-Wallis test showed a significant difference among all of the groups (p<0.05). A significant difference was also shown at every group by Mann-Whitney test (p<0.05). Meanwhile, the result of Spearman correlation test showed a significant negative correlation between the concentration of pumpkin seed extract and the Lethal Death Time as p<0.05 and coefficient correlation was -0.950. Pumpkin seed extract showed an anthelmintic effect against A. suum in vitro.

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Kakule, Ayçiçeği ve Çemen Otu Tohumları Ekstraktlarının Sazan Balığı (Cyprinus carpio L.) Sperm Motilitesine ve Oksidatif Strese In Vitro Etkileri

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v8i1.214-219.2974 ◽

2020 ◽

Vol 8 (1) ◽

pp. 214

Author(s):

Burak Evren İnanan ◽

Mahir Kanyılmaz

Keyword(s):

Oxidative Stress ◽

Common Carp ◽

Cyprinus Carpio ◽

Seed Extract ◽

Computer Assisted ◽

Fenugreek Seed ◽

Green Cardamom ◽

Motility Parameters ◽

Seed Extracts

The aim of the present study is to assess the effects Fenugreek (Trigonella foenum-graecum, Fabaceae), sunflower (Helianthus annuus, Asteraceae), green cardamom (Elettaria cardamomum, Zingiberaceae) and seed extracts, which they belonged to three different plant families, on common carp (Cyprinus carpio L.) spermatozoa motility parameters and oxidative stress conditions. For this reason, sperm samples treated with seed extracts at the ratios of 0.1%, %0.5, 1%, and 2%, were incubated at 4°C and sampled at 2nd and 48th hours. Among sperm motility parameters, curvilinear velocity (VCL), average path velocity (VAP), straight-line velocity (VSL), straightness STR), linearity (LIN), and wobble (WOB) were determined by computer-assisted sperm analysis (CASA). According to the results, sunflower and green cardamom seed extracts have attenuated motility parameters at all tested concentrations. However, motility parameters measured in %0.5 of fenugreek seed extract were higher than all other groups including the control at 48th hour. Also, TBARS values as an oxidative stress indicator in this group were decreased. 2% of all seed extracts had negative effects on the sperm samples. Particularly, 2% sunflower seed extract caused the higher oxidative stress. These results indicate that fenugreek seed extract is more proper for the maintenance of common carp spermatozoa at 4°C, comparing to those of sunflower and green cardamom.

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Effect of Homeopathic Preparations on Lettuce, Parasitized or Not by Meloidogyne enterolobii

Homeopathy ◽

10.1055/s-0040-1716402 ◽

2020 ◽

Author(s):

Thais Moraes Ferreira ◽

Mariana Zandomênico Mangeiro ◽

Alexandre Macedo Almeida ◽

Ricardo Moreira Souza

Keyword(s):

Plant Pathogens ◽

Nematode Control ◽

Plant Tolerance ◽

Nematode Reproduction ◽

Meloidogyne Enterolobii ◽

Negative Effect ◽

Meloidogyne Spp ◽

Constant Dose ◽

Lettuce Growth ◽

Complementary Strategy

Abstract Background There are relatively few scientific works on the use of homeopathy to manage plant pathogens, particularly nematodes. A handful of studies focused on Meloidogyne spp. parasitizing vegetables have brought contradictory results on nematode control and enhancement of plant tolerance to parasitism. Objective Our goal was to assess the effect of Cina—a well-known anti-nematode ingredient—on Meloidogyne enterolobii parasitizing lettuce. Methods Cina was applied daily on nematode-inoculated plants, from the seedling stage until harvest. We tested an evenly spaced range of Hahnemannian concentrations (c), which were applied though irrigation with a constant dose of the ingredient. Several absolute and relative controls were employed to allow the assessment of the effect of Cina on nematode reproduction and lettuce growth. Results Cina affected growth of non-parasitized plants, both positively and negatively; this effect was modulated by the c applied and the thermal stress suffered by the plants in one of the assays. The effect of Cina on the growth of nematode-parasitized plants was neutral or negative. Cina reduced nematode reproduction by 25–36%. Conclusion Based on the moderate negative effect of Cina on M. enterolobii reproduction, it seems this ingredient may be useful as a complementary strategy for Meloidogyne control. But Cina did not enhance the tolerance of lettuce to Meloidogyne spp.

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Evaluation of Antibacterial and Antidiabetic Potential of Silver Nanoparticles using Eugenia Uniflora L. Seed Extract

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2020.13.6.6 ◽

2020 ◽

Vol 13 (6) ◽

pp. 5217-5225

Author(s):

Guru Kumar Dugganaboyana ◽

Chethankumar Mukunda ◽

Suresh Darshini Inakanally

Keyword(s):

Biomedical Applications ◽

Pathogenic Bacteria ◽

Seed Extract ◽

Drug Formulation ◽

Visible Spectroscopy ◽

Plant Materials ◽

X Ray ◽

Eugenia Uniflora ◽

Uv Visible

In recent years, green nanotechnology-based approaches using plant materials have been accepted as an environmentally friendly and cost-effective approach with various biomedical applications. In the current study, AgNPs were synthesized using the seed extract of the Eugenia uniflora L. (E.uniflora). Characterization was done using UV-Visible spectroscopy, X-ray diffraction (XRD), scanning electronic microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) analyses. The formation of AgNPs has confirmed through UV-Visible spectroscopy (at 466 nm) by the change of color owing to surface Plasmon resonance. Based on the XRD pattern, the crystalline property of AgNPs was established. The functional group existing in seed of E.uniflora extract accountable for the reduction of Ag+ ion and the stabilization of AgNPs was investigated. The morphological structures and elemental composition was determined by SEM and EDX analysis. With the growing application of AgNPs in biomedical perspectives, the biosynthesized AgNPs were evaluated for their antibacterial and along with their antidiabetic potential. The results showed that AgNPs are extremely effective with potent antidiabetic potential at a very low concentration. It also exhibited potential antibacterial activity against the three tested human pathogenic bacteria. Overall, the results highlight the effectiveness and potential applications of AgNPs in biomedical fields such as in the treatment of acute illnesses as well as in drug formulation for treating various diseases such as cancer and diabetes. It could be concluded that E. uniflora seed extract AgNPs can be used efficiently for in vitro evaluation of their antibacterial and antidiabetic effects with potent biomedical applications.

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