scholarly journals Continuous measurements of volatile gases as detection of algae crop health

2021 ◽  
Vol 118 (40) ◽  
pp. e2106882118
Author(s):  
Jon S. Sauer ◽  
Ryan Simkovsky ◽  
Alexia N. Moore ◽  
Luis Camarda ◽  
Summer L. Sherman ◽  
...  
Keyword(s):  
Culture Collection ◽  
Gas Production ◽  
Gas Analysis ◽  
Ionization Mass ◽  
Algae Cultivation ◽  
Pasteur Culture Collection ◽  
Biomass Yields ◽  
Healthy Growth ◽  
Pcc 7942 ◽  
The Impact

Algae cultivation in open raceway ponds is considered the most economical method for photosynthetically producing biomass for biofuels, chemical feedstocks, and other high-value products. One of the primary challenges for open ponds is diminished biomass yields due to attack by grazers, competitors, and infectious organisms. Higher-frequency observations are needed for detection of grazer infections, which can rapidly reduce biomass levels. In this study, real-time measurements were performed using chemical ionization mass spectrometry (CIMS) to monitor the impact of grazer infections on cyanobacterial cultures. Numerous volatile gases were produced during healthy growth periods from freshwater Synechococcus elongatus Pasteur Culture Collection (PCC) 7942, with 6-methyl-5-hepten-2-one serving as a unique metabolic indicator of exponential growth. Following the introduction of a Tetrahymena ciliate grazer, the concentrations of multiple volatile species were observed to change after a latent period as short as 18 h. Nitrogenous gases, including ammonia and pyrroline, were found to be reliable indicators of grazing. Detection of grazing by CIMS showed indicators of infections much sooner than traditional methods, microscopy, and continuous fluorescence, which did not detect changes until 37 to 76 h after CIMS detection. CIMS analysis of gases produced by PCC 7942 further shows a complex temporal array of biomass-dependent volatile gas production, which demonstrates the potential for using volatile gas analysis as a diagnostic for grazer infections. Overall, these results show promise for the use of continuous volatile metabolite monitoring for the detection of grazing in algal monocultures, potentially reducing current grazing-induced biomass losses, which could save hundreds of millions of dollars.

scholarly journals A double-blind, placebo-controlled, cross-over study to establish the bifidogenic effect of a very-long-chain inulin extracted from globe artichoke (Cynara scolymus) in healthy human subjects

2010 ◽  
Vol 104 (7) ◽  
pp. 1007-1017 ◽  
Author(s):  
Adele Costabile ◽  
Sofia Kolida ◽  
Annett Klinder ◽  
Eva Gietl ◽  
Michael Bäuerlein ◽  
...  
Keyword(s):  
Gastrointestinal Symptoms ◽  
Gas Production ◽  
Globe Artichoke ◽  
Daily Consumption ◽  
Healthy Human ◽  
Double Blind ◽  
Cynara Scolymus ◽  
The Impact ◽  
Long Chain

There is growing interest in the use of inulins as substrates for the selective growth of beneficial gut bacteria such as bifidobacteria and lactobacilli because recent studies have established that their prebiotic effect is linked to several health benefits. In the present study, the impact of a very-long-chain inulin (VLCI), derived from globe artichoke (Cynara scolymus), on the human intestinal microbiota compared with maltodextrin was determined. A double-blind, cross-over study was carried out in thirty-two healthy adults who were randomised into two groups and consumed 10 g/d of either VLCI or maltodextrin, for two 3-week study periods, separated by a 3-week washout period. Numbers of faecal bifidobacteria and lactobacilli were significantly higher upon VLCI ingestion compared with the placebo. Additionally, levels ofAtopobiumgroup significantly increased, whileBacteroides–Prevotellanumbers were significantly reduced. No significant changes in faecal SCFA concentrations were observed. There were no adverse gastrointestinal symptoms apart from a significant increase in mild and moderate bloating upon VLCI ingestion. These observations were also confirmed byin vitrogas production measurements. In conclusion, daily consumption of VLCI extracted from globe artichoke exerted a pronounced prebiotic effect on the human faecal microbiota composition and was well tolerated by all volunteers.

Abstract 260: Knockout of Apolipoprotein A-II Has Dramatic Effects on High-Density Lipoprotein Subspecies Distribution

2012 ◽  
Vol 32 (suppl_1) ◽  
Author(s):  
Scott M Gordon ◽  
Catherine A Reardon ◽  
Godfrey S Getz ◽  
W S Davidson
Keyword(s):  
Gel Filtration ◽  
Density Lipoprotein ◽  
High Density ◽  
Paraoxonase 1 ◽  
High Density Lipoproteins ◽  
Ionization Mass ◽  
Associated Proteins ◽  
Compositional Diversity ◽  
The Impact ◽  
Modest Effect

High density lipoproteins (HDL) are a highly heterogeneous population of particles composed of various lipids and proteins. They have been demonstrated to possess a diverse variety of functional properties which are thought to contribute to protection against cardiovascular disease (CVD). Proteomics studies have identified up to 75 different proteins which can associate with HDL. The basis for the compositional diversity of HDL is not known but a better understanding will yield important information about its broad functional diversity. To investigate the impact of common HDL apolipoproteins on the distribution of other apolipoproteins, we have begun to systematically fractionate plasma from various HDL apolipoprotein KO mice. Plasma from apoA-I, apoA-IV and apoA-II global KO mice was applied to gel filtration chromatography to distinguish HDL size populations. HDL particles sequestered by a phospholipid binding resin were proteomically analyzed by electrospray ionization mass spectrometry. By comparing elution volume shifts (i.e. particle size variations) for each HDL protein between WT controls and the KO models, we assessed the impact of the deleted protein on HDL size distributions. Ablation of apoA-I, while decreasing total HDL phospholipid by 70%, had a surprisingly small impact on the distribution of the majority of other HDL associated proteins - affecting only 9 of them. Genetic apoA-IV ablation had a similar modest effect shifting a distinct subset of 9 proteins. However, loss of apoA-II, in addition to causing a similar 70% reduction in overall HDL phospholipids, affected the size distribution of some 45 HDL proteins (including several complement proteins and paraoxonase-1). These data suggest that apoA-I, while associated with the majority of HDL phospholipid, may actually interact with relatively few of the lower abundance proteins known to be associated with HDL. ApoA-II on the other hand, may interact with many of these, perhaps acting as a docking site or adaptor molecule.

Impact of the Cluster Spacing on Hydraulic Fracture Conductivity and Productivity of a Marcellus Shale Horizontal Well

2021 ◽  
Author(s):  
Mohamed El Sgher ◽  
Kashy Aminian ◽  
Ameri Samuel
Keyword(s):  
Hydraulic Fracture ◽  
Marcellus Shale ◽  
Technical Information ◽  
Gas Production ◽  
Production Performance ◽  
Valuable Insight ◽  
Fracture Conductivity ◽  
Fracture Properties ◽  
Gas Recovery ◽  
The Impact

Abstract The objective of this study was to investigate the impact of the hydraulic fracturing treatment design, including cluster spacing and fracturing fluid volume on the hydraulic fracture properties and consequently, the productivity of a horizontal Marcellus Shale well with multi-stage fractures. The availability of a significant amount of advanced technical information from the Marcellus Shale Energy and Environment Laboratory (MSEEL) provided an opportunity to perform an integrated analysis to gain valuable insight into optimizing fracturing treatment and the gas recovery from Marcellus shale. The available technical information from a horizontal well at MSEEL includes well logs, image logs (both vertical and lateral), diagnostic fracture injection test (DFIT), fracturing treatment data, microseismic recording during the fracturing treatment, production logging data, and production data. The analysis of core data, image logs, and DFIT provided the necessary data for accurate prediction of the hydraulic fracture properties and confirmed the presence and distribution of natural fractures (fissures) in the formation. Furthermore, the results of the microseismic interpretation were utilized to adjust the stress conditions in the adjacent layers. The predicted hydraulic fracture properties were then imported into a reservoir simulation model, developed based on the Marcellus Shale properties, to predict the production performance of the well. Marcellus Shale properties, including porosity, permeability, adsorption characteristics, were obtained from the measurements on the core plugs and the well log data. The Quanta Geo borehole image log from the lateral section of the well was utilized to estimate the fissure distribution s in the shale. The measured and published data were utilized to develop the geomechnical factors to account for the hydraulic fracture conductivity and the formation (matrix and fissure) permeability impairments caused by the reservoir pressure depletion during the production. Stress shadowing and the geomechanical factors were found to play major roles in production performance. Their inclusion in the reservoir model provided a close agreement with the actual production performance of the well. The impact of stress shadowing is significant for Marcellus shale because of the low in-situ stress contrast between the pay zone and the adjacent zones. Stress shadowing appears to have a significant impact on hydraulic fracture properties and as result on the production during the early stages. The geomechanical factors, caused by the net stress changes have a more significant impact on the production during later stages. The cumulative gas production was found to increase as the cluster spacing was decreased (larger number of clusters). At the same time, the stress shadowing caused by the closer cluster spacing resulted in a lower fracture conductivity which in turn diminished the increase in gas production. However, the total fracture volume has more of an impact than the fracture conductivity on gas recovery. The analysis provided valuable insight for optimizing the cluster spacing and the gas recovery from Marcellus shale.

Full scale co-digestion of organic waste

2000 ◽  
Vol 41 (3) ◽  
pp. 195-202 ◽  
Author(s):  
H. Kübler ◽  
K. Hoppenheidt ◽  
P. Hirsch ◽  
A. Kottmair ◽  
R. Nimmrichter ◽  
...  
Keyword(s):  
Negative Impact ◽  
Biogas Production ◽  
Electrical Power ◽  
Nutrient Content ◽  
Primary Energy ◽  
Gas Production ◽  
Waste Streams ◽  
Volatile Solids ◽  
Operation Results ◽  
The Impact

Operational results of a co-digestion facility were assessed over a period of 18 months. The organic fraction of municipal solid waste (OFMSW) contains a considerable amount of contaminants and grit (up to 6% w/w). A BTA-Pulper efficiently treated the different waste streams and converted a high amount of volatile solids (VS) into the digester feedstock. The seasonal fluctuations of the waste composition significantly influenced the biogas production. The impact of this seasonally variant degradability of VS had to be considered by evaluating the operation results. The waste streams investigated did not show any negative impact on digester performance. The hydraulic retention time (HRT) in the digester considerably affected the VS-reduction. Despite a considerable decrease of VS-degradation a reduction of HRT from 14 to 8 days slightly improves the gas production rate (GPR). An activated sludge system efficiently reduced the pollution of the effluent.The nutrient content of the anaerobic compostwas favourable and the content of pollutants was low. The facility produced surplus electrical power up to 290 MJ/t. An overall energy balance shows that the facility substitutes primary energy.

scholarly journals A model for coupled electro-hydro-mechanical processes in fine grained soils accounting for gas generation and transport

2010 ◽  
Vol 82 (1) ◽  
pp. 169-193 ◽  
Author(s):  
Claudio Tamagnini ◽  
Cristina Jommi ◽  
Fabio Cattaneo
Keyword(s):  
Pore Space ◽  
Transport Coefficients ◽  
Gas Production ◽  
Degree Of Saturation ◽  
Coupled Processes ◽  
Gas Generation ◽  
Fine Grained ◽  
Clayey Silt ◽  
Waste Containment Systems ◽  
The Impact

A theoretical and numerical model is developed for the quantitative analysis of coupled processes taking place in active waste containment systems, such as electrokinetic barriers or fences, in which alow intensity DC current is circulated across the clay barrier to move polar and non-polar contaminants. A novel feature of the proposed approach is the allowance for the presence of air in the pore space. Under unsaturated conditions, all transport coefficients involved in the electrokinetic process are strongly dependent on the degree of saturation of pore liquid. In order to assess the predictive capability of the proposed theory and to appreciate the impact of gas production at the electrodes, a series of numerical simulations of simple onedimensional electrokinetic tests have been performed. The results of the simulations compare reasonably well with data obtained from laboratory experiments performed on an illitic clayey silt. The numerical results indicate that the impact of gas production at the electrodes can be significant, even in low-intensity and short-duration treatments.

scholarly journals Effects of soil rewetting and thawing on soil gas fluxes: a review of current literature and suggestions for future research

2011 ◽  
Vol 8 (5) ◽  
pp. 9847-9899 ◽  
Author(s):  
D.-G. Kim ◽  
R. Vargas ◽  
B. Bond-Lamberty ◽  
M. R. Turetsky
Keyword(s):  
Climate Models ◽  
Gas Production ◽  
Global Climate Models ◽  
Soil Gas ◽  
Future Research ◽  
High Temporal Resolution ◽  
Short Term ◽  
Frozen Soils ◽  
Gas Fluxes ◽  
The Impact

Abstract. The rewetting of dry soils and the thawing of frozen soils are short-term, transitional phenomena in terms of hydrology and the thermodynamics of soil systems. The impact of these short-term phenomena on larger scale ecosystem fluxes has only recently been fully appreciated, and a growing number of studies show that these events affect various biogeochemical processes including fluxes of soil gases such as carbon dioxide (CO2), methane (CH4), nitrous oxide (N2O), ammonia (NH3) and nitric oxide (NO). Global climate models predict that future climatic change is likely to alter the frequency and intensity of drying-rewetting events and thawing of frozen soils, highlighting the importance of understanding how rewetting and thawing will influence soil gas fluxes. Here we summarize findings in a new database based on 338 studies conducted from 1956 to 2010, and highlight open research questions. The database revealed conflicting results following rewetting and thawing in various terrestrial ecosystems, ranging from large increases in gas fluxes to non-significant changes. An analysis of published field studies (n = 142) showed that after rewetting or thawing, CO2, CH4, N2O, NO and NH3 fluxes increase from pre-event fluxes following a power function, with no significant differenced among gases. We discuss possible mechanisms and controls that regulate flux responses, and note that a high temporal resolution of flux measurements is critical to capture rapid changes in gas fluxes after these soil perturbations. Finally, we propose that future studies should investigate the interactions between biological (i.e. microbial community and gas production) and physical (i.e. flux, diffusion, dissolution) changes in soil gas fluxes, and explore synergistic experimental and modelling approaches.

scholarly journals OPTIMAL PARAMETERS FOR THE FERMENTATION OF COMPLEX SUBSTRATES WHEN PRODUCING BIOGAS AT THE MAXIMAL METHANE CONCENTRATION

2014 ◽  
Vol 8 (1) ◽  
Author(s):  
Kulyash Meiramkulova ◽  
Azamat Bayanov
Keyword(s):  
Urban Areas ◽  
Factor Model ◽  
Gas Production ◽  
Maximum Amount ◽  
Energy Resources ◽  
Optimum Proportion ◽  
Fallen Leaves ◽  
The Impact ◽  
Random Nature ◽  
Fermentation Technology

Republic of Kazakhstan oil will last for 47.4 years, while gas production - 65.6 years. Given the Kashagan reserves, something else for a longer period, that is a very short periods of time with respect to the development of state and they can be extended only by the development of a technological breakthrough. Along with the question of the ever-growing needs of the population for energy resources, there is a question about the availability of annual waste generation as the impact of the rapid growth of the urban population, which is about 4% per year ("Population" encyclopedia of Astana, 2008). To cope with both problems simultaneously, the waste may be used as energy sources by fermentation technology. Among the urban wastes the organic waste is dominated, such as vegetables, fallen leaves, etc., that have the potential to produce biogas. The use of unsuitable materials as a natural catalyst of fermentation and the time spent on fermentation, are some of the existing barriers to the development of energy resources in urban areas. For this purpose a study was conducted to optimize the ratio of urban waste, animal waste and water in combination with the time of fermentation. The study used an experimental method with the random nature of the two-factor model. The first factor is the ratio of waste, manure, and the second quantity of water - during fermentation, which is fixed to each of 2, 4, 6, 8, 10, 12, 14, 16 and 18th day of the fermentation substrate. The study showed that the optimum proportion to obtain the maximum amount of biogas is a ratio of 500: 200: 300 waste, manure, and water, respectively. Furthermore, on the second day of fermentation the maximum amount of biogas produced.

scholarly journals Investigation of the Main Factors During Shale-gas Production Using Grey Relational Analysis

2016 ◽  
Vol 9 (1) ◽  
pp. 207-215 ◽  
Author(s):  
Hongling Zhang ◽  
Jing Wang ◽  
Haiyong Zhang
Keyword(s):  
Shale Gas ◽  
Gas Production ◽  
Gas Reservoirs ◽  
Fracture Conductivity ◽  
Matrix Permeability ◽  
Shale Gas Reservoirs ◽  
Main Factors ◽  
Grey Relational ◽  
The Impact ◽  
Half Length

Shale gas is one of the primary types of unconventional reservoirs to be exploited in search for long-lasting resources. Production from shale gas reservoirs requires horizontal drilling with hydraulic fracturing to achieve the most economic production. However, plenty of parameters (e.g., fracture conductivity, fracture spacing, half-length, matrix permeability, and porosity,etc) have high uncertainty that may cause unexpected high cost. Therefore, to develop an efficient and practical method for quantifying uncertainty and optimizing shale-gas production is highly desirable. This paper focuses on analyzing the main factors during gas production, including petro-physical parameters, hydraulic fracture parameters, and work conditions on shale-gas production performances. Firstly, numerous key parameters of shale-gas production from the fourteen best-known shale gas reservoirs in the United States are selected through the correlation analysis. Secondly, a grey relational grade method is used to quantitatively estimate the potential of developing target shale gas reservoirs as well as the impact ranking of these factors. Analyses on production data of many shale-gas reservoirs indicate that the recovery efficiencies are highly correlated with the major parameters predicted by the new method. Among all main factors, the impact ranking of major factors, from more important to less important, is matrix permeability, fracture conductivity, fracture density of hydraulic fracturing, reservoir pressure, total organic content (TOC), fracture half-length, adsorbed gas, reservoir thickness, reservoir depth, and clay content. This work can provide significant insights into quantifying the evaluation of the development potential of shale gas reservoirs, the influence degree of main factors, and optimization of shale gas production.

scholarly journals Be cautious during the interpretation of arterial blood gas analysis performed outside the intensive care unit

2020 ◽  
Author(s):  
Lukasz Krzych ◽  
Olga Wojnarowicz ◽  
Paweł Ignacy ◽  
Julia Dorniak
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Point Of Care ◽  
Blood Gas Analysis ◽  
Gas Analysis ◽  
Blood Gas ◽  
Acid Base Balance ◽  
Arterial Blood Gas ◽  
Arterial Blood ◽  
The Impact

Introduction. Reliable results of an arterial blood gas (ABG) analysis are crucial for the implementation of appropriate diagnostics and therapy. We aimed to investigate the differences (Δ) between ABG parameters obtained from point-of-care testing (POCT) and central laboratory (CL) measurements, taking into account the turnaround time (TAT). Materials and methods. A number of 208 paired samples were collected from 54 intensive care unit (ICU) patients. Analyses were performed using Siemens RAPIDPoint 500 Blood Gas System on the samples just after blood retrieval at the ICU and after delivery to the CL. Results. The median TAT was 56 minutes (IQR 39-74). Differences were found for all ABG parameters. Median Δs for acid-base balance ere: ΔpH=0.006 (IQR –0.0070–0.0195), ΔBEef=–0.9 (IQR –2.0–0.4) and HCO3–act=–1.05 (IQR –2.25–0.35). For ventilatory parameters they were: ΔpO2=–8.3 mmHg (IQR –20.9–0.8) and ΔpCO2=–2.2 mmHg (IQR –4.2––0.4). For electrolytes balance the differences were: ΔNa+=1.55 mM/L (IQR 0.10–2.85), ΔK+=–0.120 mM/L (IQR –0.295–0.135) and ΔCl–=1.0 mM/L (IQR –1.0–3.0). Although the Δs might have caused misdiagnosis in 51 samples, Bland-Altman analysis revealed that only for pO2 the difference was of clinical significance (mean: –10.1 mmHg, ±1.96SD –58.5; +38.3). There was an important correlation between TAT and ΔpH (R=0.45, p<0.01) with the safest time delay for proper assessment being less than 39 minutes. Conclusions. Differences between POCT and CL results in ABG analysis may be clinically important and cause misdiagnosis, especially for pO2. POCT should be advised for ABG analysis due to the impact of TAT, which seems to be the most important for the analysis of pH.

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