External carbon sources provide additional nutrients that improve the efficiency of nitrate removal in constructed wetlands. Typha angustifolia L. were planted in four vertical subsurface-flow constructed wetlands. Different external carbon sources were fed into the columns, to investigate and compare their treatment of nitrate in synthetic wastewater, with initial influent C/N ratio of 1:1. Wetland A (WA) with 50g wheat straw as external carbon source, wetland B (WB) with 50g woodchips, wetland C (WC) with additional 10mg/L glucose and wetland D (WD) without external carbon source to serve as the control, were used in the lab-scale experimental study. WA, WB, WC and WD within a period of 24 days, cumulatively removed 109.38mg/L, 93.75mg/L, 85.14mg/L, and 64.01mg/L nitrate, respectively, from the influent. The nitrate-nitrogen (NO3–N) removal efficiency as aided by the external carbon sources was in the order: wheat straw > woodchips > glucose > control. Wheat straw treated 93% NO3–N, woodchips 78%, glucose 72% and the control 53%. The results indicate that WA, WB and WC outperformed the control system, due to the additional carbon sources. In general, the wheat straw had a better performance than wood chips and glucose. Thus, wheat straw as low cost biological waste product is recommended for the treatment of nitrate in wetlands.