scholarly journals The lysosome as a command-and-control center for cellular metabolism

2016 ◽  
Vol 214 (6) ◽  
pp. 653-664 ◽  
Author(s):  
Chun-Yan Lim ◽  
Roberto Zoncu
Keyword(s):  
Building Blocks ◽  
Eukaryotic Cell ◽  
Nutrient Sensing ◽  
Cellular Growth ◽  
Metabolic Homeostasis ◽  
Waste Products ◽  
Control Center ◽  
Growth And Survival ◽  
Membrane Bound ◽  
And Control

Lysosomes are membrane-bound organelles found in every eukaryotic cell. They are widely known as terminal catabolic stations that rid cells of waste products and scavenge metabolic building blocks that sustain essential biosynthetic reactions during starvation. In recent years, this classical view has been dramatically expanded by the discovery of new roles of the lysosome in nutrient sensing, transcriptional regulation, and metabolic homeostasis. These discoveries have elevated the lysosome to a decision-making center involved in the control of cellular growth and survival. Here we review these recently discovered properties of the lysosome, with a focus on how lysosomal signaling pathways respond to external and internal cues and how they ultimately enable metabolic homeostasis and cellular adaptation.

Some Aspects of the Polyhexamethyleneguanidine Salts Effect on Cell Cultures

2014 ◽  
Vol 1 (1) ◽  
pp. 62-67 ◽  
Author(s):  
M. Mandygra ◽  
A. Lysytsia
Keyword(s):  
Proliferative Activity ◽  
Cell Monolayer ◽  
Eukaryotic Cell ◽  
Culture Methods ◽  
Cellular Processes ◽  
Negative Effect ◽  
Membrane Bound ◽  
Membrane Bound Enzymes ◽  
Anion Type ◽  
Cell Culture Methods

Aim. To investigate the effect of polyhexamethyleneguanidine (PHMG) to eukaryotic cell culture. Methods. The passaged bovine tracheal cells culture (TCC) and primary culture of chicken embryo fi broblasts (FCE) were used in the experiments. TCC and FCE monolayers were treated with aqueous solutions of PHMG chloride or succinate. The method of PHMG polycation adsorption to the cells’ plasma membrane together with microscopy were applied. Results. The dependence of PHMG effect on the eukaryotic cells on the agent concentration, duration of exposure and the anion type has been fi xed. The PHMG concentration of 10 –5 per cent (0.1 μg/ml) never causes degradation of the previously formed cell monolayer, while the higher concentrations damage it. The conditions of the PHMG chloride and succinate’s negative effect on cell proliferation and inhibition of monolayer formation were determined. The hypothesis that under certain conditions PHMG stimulates the proliferative activity of the cells has been confi rmed. Stimulation may be associated with non-specifi c stress adaptation of cells. In this case, it is due to modifi cations of the cell membrane after PHMG adsorption to it. Conclusions. PHMG polycation binds with the membrane’s phosphoglycerides fi rmly and irreversibly. A portion of the lipids are removed from participation in the normal cellular processes at that. At the same time, the synthesis of new lipids and membrane-bound enzymes is probably accelerated. The phospholip ids’ neogenesis acceleration can stimulate mitosis under certain conditions. The obtained results can be used in the biotechnologies.

scholarly journals Wolbachia: endosymbiont of onchocercid nematodes and their vectors

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Ranju Ravindran Santhakumari Manoj ◽  
Maria Stefania Latrofa ◽  
Sara Epis ◽  
Domenico Otranto
Keyword(s):  
Control Strategies ◽  
Molecular Data ◽  
Scientific Data ◽  
Growth And Survival ◽  
Vector Borne Diseases ◽  
Wide Range ◽  
Veterinary Importance ◽  
Wolbachia Endosymbiont ◽  
Vector Borne ◽  
And Control

Abstract Background Wolbachia is an obligate intracellular maternally transmitted, gram-negative bacterium which forms a spectrum of endosymbiotic relationships from parasitism to obligatory mutualism in a wide range of arthropods and onchocercid nematodes, respectively. In arthropods Wolbachia produces reproductive manipulations such as male killing, feminization, parthenogenesis and cytoplasmic incompatibility for its propagation and provides an additional fitness benefit for the host to protect against pathogens, whilst in onchocercid nematodes, apart from the mutual metabolic dependence, this bacterium is involved in moulting, embryogenesis, growth and survival of the host. Methods This review details the molecular data of Wolbachia and its effect on host biology, immunity, ecology and evolution, reproduction, endosymbiont-based treatment and control strategies exploited for filariasis. Relevant peer-reviewed scientic papers available in various authenticated scientific data bases were considered while writing the review. Conclusions The information presented provides an overview on Wolbachia biology and its use in the control and/or treatment of vectors, onchocercid nematodes and viral diseases of medical and veterinary importance. This offers the development of new approaches for the control of a variety of vector-borne diseases. Graphic Abstract

scholarly journals Subgraphs of Interest Social Networks for Diffusion Dynamics Prediction

Entropy ◽  
2021 ◽  
Vol 23 (4) ◽  
pp. 492
Author(s):  
Valentina Y. Guleva ◽  
Polina O. Andreeva ◽  
Danila A. Vaganov
Keyword(s):  
Social Networks ◽  
Sampling Method ◽  
Building Blocks ◽  
Global Dynamics ◽  
Diffusion Dynamics ◽  
Different Types ◽  
Prediction And Control ◽  
Path Lengths ◽  
And Control ◽  
Diffusion Prediction

Finding the building blocks of real-world networks contributes to the understanding of their formation process and related dynamical processes, which is related to prediction and control tasks. We explore different types of social networks, demonstrating high structural variability, and aim to extract and see their minimal building blocks, which are able to reproduce supergraph structural and dynamical properties, so as to be appropriate for diffusion prediction for the whole graph on the base of its small subgraph. For this purpose, we determine topological and functional formal criteria and explore sampling techniques. Using the method that provides the best correspondence to both criteria, we explore the building blocks of interest networks. The best sampling method allows one to extract subgraphs of optimal 30 nodes, which reproduce path lengths, clustering, and degree particularities of an initial graph. The extracted subgraphs are different for the considered interest networks, and provide interesting material for the global dynamics exploration on the mesoscale base.

Modeling human-like longitudinal driver model for intelligent vehicles based on reinforcement learning

2021 ◽  
pp. 095440702098357
Author(s):  
Ju Xie ◽  
Xing Xu ◽  
Feng Wang ◽  
Haobin Jiang
Keyword(s):  
Reinforcement Learning ◽  
Comprehensive Evaluation ◽  
Path Following ◽  
Intelligent Vehicles ◽  
Driver Model ◽  
Control Center ◽  
Training Performance ◽  
Learning Agents ◽  
System A ◽  
And Control

The driver model is the decision-making and control center of intelligent vehicle. In order to improve the adaptability of intelligent vehicles under complex driving conditions, and simulate the manipulation characteristics of the skilled driver under the driver-vehicle-road closed-loop system, a kind of human-like longitudinal driver model for intelligent vehicles based on reinforcement learning is proposed. This paper builds the lateral driver model for intelligent vehicles based on optimal preview control theory. Then, the control correction link of longitudinal driver model is established to calculate the throttle opening or brake pedal travel for the desired longitudinal acceleration. Moreover, the reinforcement learning agents for longitudinal driver model is parallel trained by comprehensive evaluation index and skilled driver data. Lastly, training performance and scenarios verification between the simulation experiment and the real car test are performed to verify the effectiveness of the reinforcement learning based longitudinal driver model. The results show that the proposed human-like longitudinal driver model based on reinforcement learning can help intelligent vehicles effectively imitate the speed control behavior of the skilled driver in various path-following scenarios.

scholarly journals Self-reproducing catalyst drives repeated phospholipid synthesis and membrane growth

2015 ◽  
Vol 112 (27) ◽  
pp. 8187-8192 ◽  
Author(s):  
Michael D. Hardy ◽  
Jun Yang ◽  
Jangir Selimkhanov ◽  
Christian M. Cole ◽  
Lev S. Tsimring ◽  
...  
Keyword(s):  
Lipid Synthesis ◽  
Building Blocks ◽  
Phospholipid Bilayer ◽  
Phospholipid Synthesis ◽  
Synthetic Membranes ◽  
Design And Synthesis ◽  
Membrane Growth ◽  
Membrane Bound ◽  
Living Organisms ◽  
Dynamic Structures

Cell membranes are dynamic structures found in all living organisms. There have been numerous constructs that model phospholipid membranes. However, unlike natural membranes, these biomimetic systems cannot sustain growth owing to an inability to replenish phospholipid-synthesizing catalysts. Here we report on the design and synthesis of artificial membranes embedded with synthetic, self-reproducing catalysts capable of perpetuating phospholipid bilayer formation. Replacing the complex biochemical pathways used in nature with an autocatalyst that also drives lipid synthesis leads to the continual formation of triazole phospholipids and membrane-bound oligotriazole catalysts from simpler starting materials. In addition to continual phospholipid synthesis and vesicle growth, the synthetic membranes are capable of remodeling their physical composition in response to changes in the environment by preferentially incorporating specific precursors. These results demonstrate that complex membranes capable of indefinite self-synthesis can emerge when supplied with simpler chemical building blocks.

Preparedness for Mitigating Noncommunicable Diseases in Gaindakot Municipality, Nepal: Perspectives of Key informants

2021 ◽  
Vol 6 (1) ◽  
pp. 1320-1324
Author(s):  
Narayan Sapkota ◽  
Damaru Prasad Paneru
Keyword(s):  
Health System ◽  
Health Workforce ◽  
Prevention And Control ◽  
Local Level ◽  
Building Blocks ◽  
Health System Strengthening ◽  
High Morbidity ◽  
Major Public Health Problem ◽  
Screening Services ◽  
And Control

Introduction: Non-communicable Diseases (NCDs) are the major public health problem that leads to high morbidity and mortality in the world including Nepal. Government of Nepal has launched the Multi-sectoral NCD Action Plan in 2014 and established NCD and Injuries Poverty Commission in 2016 for the management and control of NCDs nevertheless the implementation status and its outcomes are not identified till date at the local level. Objectives: To explore the preparedness of the local government for the prevention and control of NCDs at Gaindakot, Nawalpur, Nepal. Methodology: A qualitative study was conducted in the Gaindakot municipality; Nawalpur to document the key informant's perspectives on health system's preparedness to prevent the potential impacts of NCDs. Face to face Indepth interview was performed using open-ended questions. Interview guidelines were prepared on the basis of building blocks of health system. Information was processed basis on thematic analysis. Result: The study revealed that health section has NCD preparedness structure but need to strengthening for the better delivery of health services. The study highlights that screening services and the medicine for major NCDs like hypertension and diabetes were available at local level. Limited budget was allocated and health workforce was not trained for NCDs prevention and control. There was no reporting mechanism for NCD related data from local level. Conclusion: Basic medicine and screening services were provided from the local level to the selected NCDs such as hypertension and diabetes. There was no provision of reporting NCD related information and health workforce were not trained to respond NCDs. Local level health system strengthening is an urgent need to address the increasing burden of NCDs.  

scholarly journals Specific Heterodimer Formation Is a Prerequisite for Uroplakins to Exit from the Endoplasmic Reticulum

2002 ◽  
Vol 13 (12) ◽  
pp. 4221-4230 ◽  
Author(s):  
Liyu Tu ◽  
Tung-Tien Sun ◽  
Gert Kreibich
Keyword(s):  
Endoplasmic Reticulum ◽  
Plasma Membrane ◽  
Posttranslational Modifications ◽  
Cell Layer ◽  
Building Blocks ◽  
Apical Plasma Membrane ◽  
Membrane Bound ◽  
Umbrella Cells ◽  
Vesicular Compartment ◽  
Lower Urinary

Much of the lower urinary tract, including the bladder, is lined by a stratified urothelium forming a highly differentiated, superficial umbrella cell layer. The apical plasma membrane as well as abundant cytoplasmic fusiform vesicles of the umbrella cells is covered by two-dimensional crystals that are formed by four membrane proteins named uroplakins (UPs) Ia, Ib, II, and III. UPs are synthesized on membrane-bound polysomes, and after several co- and posttranslational modifications they assemble into planar crystals in a post-Golgi vesicular compartment. Distension of the bladder may cause fusiform vesicles to fuse with the apical plasma membrane. We have investigated the early stages of uroplakin assembly by expressing the four uroplakins in 293T cells. Transfection experiments showed that, when expressed individually, only UPIb can exit from the endoplasmic reticulum (ER) and move to the plasma membrane, whereas UPII and UPIII reach the plasma membrane only when they form heterodimeric complexes with UPIa and UPIb, respectively. Heterodimer formation in the ER was confirmed by pulse-chase experiment followed by coimmunoprecipitation. Our results indicate that the initial building blocks for the assembly of crystalline uroplakin plaques are heterodimeric uroplakin complexes that form in the ER.

scholarly journals Sucralose induced pancreatic toxicity in albino rats: Histomorphological evidence

2014 ◽  
Vol 31 (02) ◽  
pp. 123-127 ◽  
Author(s):  
S. Gupta ◽  
S. Kalra ◽  
V. Bharihoke ◽  
D. Dhurandhar
Keyword(s):  
Lethal Dose ◽  
Nutrient Sensing ◽  
Albino Rats ◽  
Artificial Sweetener ◽  
Distilled Water ◽  
Obesity And Diabetes ◽  
Pancreatic Toxicity ◽  
Treatment Of Obesity ◽  
Wistar Albino Rats ◽  
And Control

AbstractIn the present world people have become conscious of the fact that sugar is one of the commonest culprits for gain in weight and blood sugar in diabetes. One of the artificial sweetener, sucralose is widely used by diabetics is still under strict scrutiny because of the contradictory feedbacks obtained from various studies, often discouraging its use. Therefore, there arises a need to check whether the use of sucralose is safe or not. The present study was designed to determine to evaluate and compare the histological changes of sucralose on the islets of pancreas in albino rats. Methods: The adult Wistar albino rats were given sucralose orally by gavage in the dose of 3gms/kg body weight/day dissolved in distilled water and only distilled water for 30 days to experimental and control groups respectively. The animals were weighed prior to and after the experiment. The animals were sacrificed on day 31. The pancreas was dissected and observed grossly. Tissue was processed; paraffin blocks were prepared and 8 micron thick sections were cut. Sections were stained with Haemotoxylin and Eosin and Gomori's stains. Results: Pancreatic toxicity was observed in the form of vaculation of islets, lymphocytic infilterate, degeneration of islets and acini of Pancreas. The commonly used non-lethal dose of sucralose induced damage to Pancreas is startling. Conclusion: The results obtained have wide implications for nutrient sensing and nutrition in the treatment of obesity and diabetes.

Growth and Survival of Hormone-Sterilized Coho (Oncorhynchus kisutch) and Kokanee Salmon (O. nerka) in a Lacustrine Environment

1988 ◽  
Vol 45 (8) ◽  
pp. 1490-1494 ◽  
Author(s):  
Eric A. Parkinson ◽  
Kanji Tsumura
Keyword(s):  
Survival Rates ◽  
Oncorhynchus Kisutch ◽  
Control Fish ◽  
Lacustrine Environment ◽  
Growth And Survival ◽  
Kokanee Salmon ◽  
Return Rates ◽  
Differential Vulnerability ◽  
Normal Life Span ◽  
And Control

Coho (Oncorhynchus kisutch) and kokanee salmon (O. nerka) were sterilized using 17α-methyltestosterone treatments and released into a lacustrine environment. The treatment appeared to be successful and sizes at capture were similar for treated and control fish. Return rates of hormone-treated groups of fish were much lower than those of the control groups. Differences in return rates were assumed to be due to differences in survival rates but the possibility of differential vulnerability to the capture gear could not be ruled out. The proportion of treated kokanee in the catch increased with age, indicating that treated kokanee survived beyond the normal life span of the species.

Research and Development of CVIS Testing System at Road Intersection Based on ZigBee

2013 ◽  
Vol 462-463 ◽  
pp. 505-509 ◽  
Author(s):  
Hao Zhang
Keyword(s):  
Testing System ◽  
Traffic Light ◽  
Interactive Communication ◽  
Control Center ◽  
Time Period ◽  
Moving Vehicles ◽  
Traffic Light Control ◽  
Infrastructure System ◽  
Feedback Data ◽  
And Control

The traditional traffic light control system which applies timer control cannot adjust the light time period effectively when the vehicle flow changes. Based on the Cooperative Vehicle Infrastructure System (CVIS), this paper puts forward a scheme that traffic light can be changed adaptively according to the vehicle flow. It realizes the interactive communication among moving vehicles, roadside equipments and control center. As a result, the traffic light time period is regulated by the real-time feedback data.

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