scholarly journals Two S. pombe septation phases differ in ingression rate, septum structure, and response to F-actin loss

2019 ◽  
Vol 218 (12) ◽  
pp. 4171-4194 ◽  
Author(s):  
Mariona Ramos ◽  
Juan Carlos G. Cortés ◽  
Mamiko Sato ◽  
Sergio A. Rincón ◽  
M. Belén Moreno ◽  
...  
Keyword(s):  
Fission Yeast ◽  
Dual Function ◽  
Second Phase ◽  
Glucan Synthase ◽  
Two Phases ◽  
Anaphase B ◽  
The Relationship ◽  
Contractile Structure ◽  
In Cells

In fission yeast, cytokinesis requires a contractile actomyosin ring (CR) coupled to membrane and septum ingression. Septation proceeds in two phases. In anaphase B, the septum ingresses slowly. During telophase, the ingression rate increases, and the CR becomes dispensable. Here, we explore the relationship between the CR and septation by analyzing septum ultrastructure, ingression, and septation proteins in cells lacking F-actin. We show that the two phases of septation correlate with septum maturation and the response of cells to F-actin removal. During the first phase, the septum is immature and, following F-actin removal, rapidly loses the Bgs1 glucan synthase from the membrane edge and fails to ingress. During the second phase, the rapidly ingressing mature septum can maintain a Bgs1 ring and septum ingression without F-actin, but ingression becomes Cdc42 and exocyst dependent. Our results provide new insights into fungal cytokinesis and reveal the dual function of CR as an essential landmark for the concentration of Bgs1 and a contractile structure that maintains septum shape and synthesis.

scholarly journals The Relationship Between English Language Proficiency and Politeness in Making Requests: A Case Study of ESL Saudi Students

2019 ◽  
Vol 9 (2) ◽  
pp. 89
Author(s):  
Abdullah Alshakhi
Keyword(s):  
Language Proficiency ◽  
English Language ◽  
English Proficiency ◽  
The United States ◽  
English Language Proficiency ◽  
Language Learner ◽  
Second Phase ◽  
Saudi Students ◽  
Two Phases ◽  
The Relationship

The acquisition of language as well as the acquisition of social consideration, or politeness, are two sets of separate skills. However, the lack of language knowledge by an English language learner can result in social mistakes that can be perceived as impolite by native speakers. The present study aimed to explore the relationship between English language proficiency and politeness in making requests at retail shops, specifically focusing on Saudi students in the United States. There were five participants in this study: two Saudi ESL students with low English proficiency; two Saudi Ph. D. students with advanced English proficiency, and an American waiter who speaks English as a native language. The study was conducted by using a qualitative research method in two phases. The first phase included observing all participants during interaction, and the second phase included interviewing a server at one of the restaurants where the students visited. The main findings revealed that there was no strong correlation between politeness and English language proficiency in making requests. However, there were some factors found in this study that contributed to politeness level, such as intonations and the use of politeness markers.

scholarly journals Intramitotic controls in the fission yeast Schizosaccharomyces pombe: the effect of cell size on spindle length and the timing of mitotic events.

1990 ◽  
Vol 110 (5) ◽  
pp. 1617-1621 ◽  
Author(s):  
I M Hagan ◽  
P N Riddle ◽  
J S Hyams
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Average Length ◽  
Nuclear Migration ◽  
Yeast Cells ◽  
Reverse Direction ◽  
Wild Type ◽  
Spindle Elongation ◽  
Anaphase B ◽  
In Cells

We have used a new cinemicroscopy technique in combination with antitubulin immunofluorescence microscopy to investigate the timing of mitotic events in cells of the fission yeast Schizosaccharomyces pombe having lengths at division between 7 and 60 microns. Wild-type fission yeast cells divide at a length of 14 microns. Separation of daughter nuclei (anaphase B) proceeds at a rate of 1.6 +/- 0.2 microns min-1, until the spindle extends the length of the cell. Coincident with spindle depolymerization, the nuclei reverse direction and take up positions that will become the center of the two daughter cells. This post-mitotic nuclear migration occurs at a rate of 1.4 +/- 0.5 microns-1. In cells in which the weel+ gene is overexpressed fivefold and that have an average length at mitosis of 28 microns, the rate of nuclear separation was only slightly reduced but, as spindles in these cells measure 20-22 microns, the duration of anaphase B was extended by approximately 40%. By contrast, in the mutant weel.50, which divides at 7 microns, both the rate and duration of anaphase B were indistinguishable from wild type. Nuclei reach the ends of these cells earlier but remain there until a point corresponding to the time of postmitotic nuclear migration in wild type. Thus, the events of mitosis can be extended but not abbreviated. These results are discussed in terms of a mitotic termination control that monitors many different events, one of which is spindle elongation.

scholarly journals Cross-Modal Perceptual Organization in Works of Art

i-Perception ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 204166952095075
Author(s):  
Liliana Albertazzi ◽  
Luisa Canal ◽  
Rocco Micciolo ◽  
Iacopo Hachen
Keyword(s):  
Semantic Differential ◽  
Second Phase ◽  
Abstract Concepts ◽  
Piano Works ◽  
Musical Excerpts ◽  
Bipolar Scale ◽  
Two Phases ◽  
Perceptual Characteristics ◽  
The Relationship ◽  
Differential Test

This study investigates the existence of cross-modal correspondences between a series of paintings by Kandinsky and a series of selections from Schönberg music. The experiment was conducted in two phases. In the first phase, by means of the Osgood semantic differential, the participants evaluated the perceptual characteristics first of visual stimuli (some pictures of Kandinsky’s paintings, with varying perceptual characteristics and contents) and then of auditory stimuli (musical excerpts taken from the repertoire of Schönberg’s piano works) relative to 11 pairs of adjectives tested on a continuous bipolar scale. In the second phase, participants were required to associate pictures and musical excerpts. The results of the semantic differential test show that certain paintings and musical excerpts were evaluated as semantically more similar, while others were evaluated as semantically more different. The results of the direct association between musical excerpts and paintings showed both attractions and repulsions among the stimuli. The overall results provide significant insights into the relationship between concrete and abstract concepts and into the process of perceptual grouping in cross-modal phenomena.

scholarly journals EMG activity in hyoid muscles during pig suckling

2012 ◽  
Vol 112 (9) ◽  
pp. 1512-1519 ◽  
Author(s):  
A. J. Thexton ◽  
A. W. Crompton ◽  
R. Z. German
Keyword(s):  
Emg Activity ◽  
Cycle Period ◽  
Second Phase ◽  
Cycle Duration ◽  
Fixed Duration ◽  
Cervical Plexus ◽  
Pharyngeal Swallow ◽  
Two Phases ◽  
Pharyngeal Swallowing ◽  
The Relationship

Infant suckling is a complex behavior that includes cycles of rhythmic sucking as well as intermittent swallows. This behavior has three cycle types: 1) suck cycles, when milk is obtained from the teat and moved posteriorly into the valleculae in the oropharynx; 2) suck-swallow cycles, which include both a rhythmic suck and a pharyngeal swallow, where milk is moved out of the valleculae, past the larynx, and into the esophagus; and 3) postswallow suck cycles, immediately following the suck-swallow cycles. Because muscles controlling these behaviors are active in all three types of cycles, we tested the hypothesis that different patterns of electromyographic (EMG) activity in the mylohyoid, hyoglossus, stylohyoid, and thyrohyoid muscles of the pig characterized each cycle type. Anterior mylohyoid EMG activity occurred regularly in every cycle and was used as a cycle marker. Thyrohyoid activity, indicating the pharyngeal swallow, was immediately preceded by increased stylohyoid and hyoglossus activity; it divided the suck-swallow cycle into two phases. Timed from the onset of the suck-swallow cycle, the first phase had a relatively fixed duration while the duration of the second phase, timed from the thyrohyoid, varied directly with cycle duration. In short-duration cycles, the second phase could have a zero duration so that thyrohyoid activity extended into the postswallow cycle. In such cycles, all swallowing activity that occurred after the thyrohyoid EMG and was associated with bolus passage through the pharynx fell into the postswallow cycle. These data suggest that while the activity of some muscles, innervated by trigeminal and cervical plexus nerves, may be time locked to the cycle onset in swallowing, the cycle period itself is not. The postswallow cycle consequently contains variable amounts of pharyngeal swallowing EMG activity. The results exemplify the complexity of the relationship between rhythmic sucking and the swallow.

Lactate and contractile force in frog muscle during development of fatigue and recovery

1976 ◽  
Vol 231 (2) ◽  
pp. 430-433 ◽  
Author(s):  
RH Fitts ◽  
JO Holloszy
Keyword(s):  
Lactate Concentration ◽  
Contractile Force ◽  
Sartorius Muscle ◽  
Second Phase ◽  
Initial Value ◽  
Muscle Lactate ◽  
Total Recovery ◽  
Two Phases ◽  
The Relationship ◽  
Frog Sartorius

The relationship between lactic acid concentration and twitch tension was reevaluated in electrically stimulated frog sartorius muscle. In muscles stimulated under anaerobic conditions at a rate of 30 stimuli/MIN CONTRACTILE FORCE DECREASED TO 36% OF THE INITIAL VALUE IN 15 MIN, Concomitantly lactate increased from 3.3 to 18.7 mumol/g of muscle. The correlaiton between the increase in lactate and the decrease in contractile force was significant (r = -0.99, P less than 0.000001). Recovery occurred in two phases. A rapid increase in contractile force, which represented 20% of the total recovery, took place during the first 15 s and occurred concomitantly with an increase in ATP from 3.9 to 4.6 mumol/g. Lactate concentration did not change significantly during this period. The second phase of recovery of contractile force was complete in 50 min. Lactate concentration and contractile force were significatly correlated during recovery (r = -0;92, P less than 0.00001). However, recovery of contractile force lagged behind the decrease in lactate; a given concentration of muscle lactate was associated with a higher contractile force early during development of fatigue than late during recovery.

scholarly journals Educational actions in human communication health: telehealth contributions in primary care

Revista CEFAC ◽  
2018 ◽  
Vol 20 (2) ◽  
pp. 182-190 ◽  
Author(s):  
Gabriela Guedes de Sá Leitão ◽  
Tatiana de Paula Santana da Silva ◽  
Maria Luiza Lopes Timothy Lima ◽  
Mirella Rodigues ◽  
Cynthia Barboza Maria Nascimento
Keyword(s):  
Primary Care ◽  
Health Center ◽  
Cross Sectional Study ◽  
Descriptive Statistics ◽  
Second Phase ◽  
Sectional Study ◽  
Human Communication ◽  
Cross Sectional ◽  
Two Phases ◽  
The Relationship

ABSTRACT Objective: to characterize educational actions related to human communication health produced at the Tele-Health Center for health professionals in primary care. Methods: a cross-sectional study was conducted at the Tele-Health Center at the Federal University of Pernambuco Clinical Hospital. Educational actions produced by tele-consultants between 2008 and 2014 linked to the health of human communication were considered. Data collection was conducted in two phases. In the first phase, the data were explored and educational actions were selected based on the title and the relationship with human communication. In the second phase, each action was observed and evaluated for content. The data were analyzed using descriptive statistics. Results: a few educational actions related to human communication health were concentrated in 2014. Throughout the period analyzed, the actions were restricted to the field of language and concentrated on the education issue as well as the strategic area of child and adolescent health. The most frequent occupational category among the tele-consultants was nursing. Conclusion: a small number of educational actions addressing the health of human communication was produced and the participation of speech therapists remains incipient.

scholarly journals Microinjection of biotin-tubulin into anaphase cells induces transient elongation of kinetochore microtubules and reversal of chromosome-to-pole motion.

1992 ◽  
Vol 116 (6) ◽  
pp. 1409-1420 ◽  
Author(s):  
E Shelden ◽  
P Wadsworth
Keyword(s):  
Electron Microscopic ◽  
Electron Microscopic Immunocytochemistry ◽  
Pole Motion ◽  
Anaphase Onset ◽  
Tubulin Subunit ◽  
Confocal Fluorescence ◽  
Anaphase B ◽  
The Relationship ◽  
Chromosome Motion ◽  
In Cells

During prometaphase and metaphase of mitosis, tubulin subunit incorporation into kinetochore microtubules occurs proximal to the kinetochore, at the plus-ends of kinetochore microtubules. During anaphase, subunit loss from kinetochore fiber microtubules is also thought to occur mainly from microtubule plus-ends, proximal to the kinetochore. Thus, the kinetochore can mediate both subunit addition and loss while maintaining an attachment to kinetochore microtubules. To examine the relationship between chromosome motion and tubulin subunit assembly in anaphase, we have injected anaphase cells with biotin-labeled tubulin subunits. The pattern of biotin-tubulin incorporation was revealed using immunoelectron and confocal fluorescence microscopy of cells fixed after injection; chromosome motion was analyzed using video records of living injected cells. When anaphase cells are examined approximately 30 s after injection with biotin-tubulin, bright "tufts" of fluorescence are detected proximal to the kinetochores. Electron microscopic immunocytochemistry further reveals that these tufts of biotin-tubulin-containing microtubules are continuous with unlabeled kinetochore fiber microtubules. Biotin-tubulin incorporation proximal to the kinetochore in anaphase cells is detected after injection of 3-30 mg/ml biotin-tubulin, but not in cells injected with 0.3 mg/ml biotin-tubulin. At intermediate concentrations of biotin-tubulin (3-5 mg/ml), incorporation at the kinetochore can be detected within 15 s after injection; by approximately 1 min after injection discrete tufts of fluorescence are no longer detected, although some incorporation throughout the kinetochore fiber and into nonkinetochore microtubules is observed. At higher concentrations of injected biotin-tubulin (13 mg/ml), incorporation at the kinetochore is more extensive and occurs for longer periods of time than at intermediate concentrations. Incorporation of biotin-tubulin proximal to the kinetochore can be detected in cells injected during anaphase A, but not during anaphase B. Analysis of video records of microinjection experiments reveals that kinetochore proximal incorporation of biotin-tubulin is accompanied by a transient reversal of chromosome-to-pole motion. Chromosome motion is not altered after injection of 0.3 mg/ml biotin-tubulin or 5 mg/ml BSA. These results demonstrate that kinetochore microtubules in anaphase cells can elongate in response to the elevation of the tubulin concentration and that kinetochores retain the ability to mediate plus-end-dependent assembly of KMTs and plus-end-directed chromosome motion after anaphase onset.

Accurate Detection and Quantization of Leaf- Diseases through Soft Computing

2018 ◽  
Vol 1 (1) ◽  
pp. 236-247
Author(s):  
Divya Srivastava ◽  
Rajitha B. ◽  
Suneeta Agarwal
Keyword(s):  
Machine Learning ◽  
Image Processing ◽  
Agricultural Production ◽  
Bacterial Blight ◽  
Early Stage ◽  
Second Phase ◽  
Computationally Efficient ◽  
Stage Of Disease ◽  
Accurate Detection ◽  
Two Phases

Diseases in leaves can cause the significant reduction in both quality and quantity of agricultural production. If early and accurate detection of disease/diseases in leaves can be automated, then the proper remedy can be taken timely. A simple and computationally efficient approach is presented in this paper for disease/diseases detection on leaves. Only detecting the disease is not beneficial without knowing the stage of disease thus the paper also determine the stage of disease/diseases by quantizing the affected of the leaves by using digital image processing and machine learning. Though there exists a variety of diseases on leaves, but the bacterial and fungal spots (Early Scorch, Late Scorch, and Leaf Spot) are the most prominent diseases found on leaves. Keeping this in mind the paper deals with the detection of Bacterial Blight and Fungal Spot both at an early stage (Early Scorch) and late stage (Late Scorch) on the variety of leaves. The proposed approach is divided into two phases, in the first phase, it identifies one or more disease/diseases existing on leaves. In the second phase, amount of area affected by the disease/diseases is calculated. The experimental results obtained showed 97% accuracy using the proposed approach.

Representação e simbolismo: artes visuais na fronteira Brasil/Bolívia

2020 ◽  
Vol 4 (3) ◽  
pp. 286
Author(s):  
Paulo César Antonini de Souza ◽  
Derick Trindade Bezerra
Keyword(s):  
Visual Arts ◽  
Social Order ◽  
Research Field ◽  
Border Region ◽  
Second Phase ◽  
Two Dimensional ◽  
Santa Cruz ◽  
Cultural Conditions ◽  
Two Phases ◽  
America Latina

ResumoTendo por campo de investigação o Festival da América do Sul Pantanal (FASP) em 2018, na cidade de Corumbá (Brasil), objetiva-se identificar a materialidade e conceitos que permeiam as manifestações artísticas bidimensionais nesta região de fronteira, a partir da percepção de artistas da Bolívia. A pesquisa se organizou em duas fases: na primeira foi realizado um levantamento em plataformas online de produções acadêmicas em artes visuais, com foco no trabalho bidimensional, utilizando os descritores “arte popular” e “estética latina” resultando em três artigos. Na segunda fase foram selecionados dois trabalhos de uma artista da Bolívia, participante da mostra “Conexão Santa Cruz”, realizada durante o FASP 2018, que foram analisados em seus níveis representacional e simbólico. Pela interpretação das imagens foi possível construir uma leitura sobre a perspectiva da artista a respeito de suas condições culturais dentro da ordenação social em que se encontra situada.Palavras-chave: Artes Visuais. Arte Popular. Arte Regional. América Latina. Representation and symbolism: visual arts on the Brazil/Bolivia frontierAbstractHaving as research field the Festival da América do Sul Pantanal (FASP) in 2018, in the city of Corumbá (Brazil), the objective is to identify the materiality and concepts that permeate the two-dimensional artistic manifestations in this border region, from the perception of artists from Bolivia. The research was organized in two phases: in the first, a survey was carried out on online platforms of academic productions in visual arts, focusing on two-dimensional work, using the descriptors “arte popular” and “estética latina” resulting in three articles. In the second phase, two works were selected by an artist from Bolivia, participating in the exhibition “Conexão Santa Cruz”, held during FASP 2018, which were analyzed at their representational and symbolic levels. Through the interpretation of the images, it was possible to construct a reading on the artist’s perspective regarding her cultural conditions within the social order in which she is located.Keywords: Visual Arts. Folk Art. Regional Art. Latin America.Representación y simbolismo: artes visuales en la frontera de Brasil/BoliviaResumenTeniendo como campo de investigación el Festival de Sudamérica Pantanal (FASP) en 2018, en la ciudad de Corumbá (Brasil), el objetivo es identificar la materialidad y conceptos que permean las manifestaciones artísticas bidimensionales en esta región fronteriza, desde la percepción de artistas de Bolivia. La investigación se organizó en dos fases: en la primera, se realizó una encuesta en plataformas online de producciones académicas en artes visuales, con foco en el trabajo bidimensional, utilizando los descriptores “arte popular” y “estética latina” dando como resultado tres artículos. En la segunda fase, dos obras fueron seleccionadas por un artista de Bolivia, participante de la exposición “Conexão Santa Cruz”, realizada durante FASP 2018, que fueron analizadas en sus niveles representativos y simbólicos. A través de la interpretación de las imágenes, fue posible construir una lectura sobre la perspectiva de la artista sobre sus condiciones culturales dentro del orden social en el que se ubica.Palabras clave: Artes Visuales. Arte Popular. Arte Regional. América Latina.

Introduction

2018 ◽  
Author(s):  
Chris Jones
Keyword(s):  
Nineteenth Century ◽  
Old English ◽  
English Studies ◽  
Anglo Saxon ◽  
Political Events ◽  
Two Phases ◽  
Scholarly Contribution ◽  
Anglophone World ◽  
The Relationship

This introductory chapter contextualizes the philological study of language during the nineteenth century as a branch of the evolutionary sciences. It sketches in outline the two phases of poetic Anglo-Saxonism for which the rest of the book will subsequently argue in more detail. Moreover, the relationship between Anglo-Saxonism and nineteenth-century medievalism more generally is articulated, and historical analogies are drawn between nineteenth-century Anglo-Saxonism and more recent political events in the Anglophone world. Finally, the scholarly contribution of Fossil Poetry itself is contextualized within English Studies; it is argued that ‘reception’ is one of the primary objects of Anglo-Saxon or Old English studies, and not merely a secondary object of that field’s study.

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