INTERACTIONS OF C-REACTIVE PROTEIN WITH THE COMPLEMENT SYSTEM

Protamine sulfate was found to consume large amounts of C selectively during preincubation with sera of individuals in the "acute phase". Marked depletion of C1, C4, and C2 with minimal, if any, depletion of C3-9, was observed. The consumption was time and temperature dependent, occurring most rapidly and extensively at 37°C, 0.10 M relative salt concentration and pH 7.5–8.0; it required calcium ions. It was mediated by a heat-stable nondialyzable factor which separated with C-reactive protein (CRP) during fractionation and purification, correlated with serum CRP levels, and, like other known reactivities of CRP, was inhibited by phosphoryl choline. Preparations of CRP purified either from serum or ascites resulted in consumption of large amounts of C1, C4, and C2 when preincubated with normal serum and protamine. We conclude that CRP is a potent activator of the C system at the level of C1, and that polycations such as protamine sulfate are substrates of CRP which can bring about this activation. It seems not unlikely that one role of CRP in health and disease involves its ability to interact with the C system.

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Interactions of C-reactive protein with the complement system. II. C-reactive protein-mediated consumption of complement by poly-L-lysine polymers and other polycations.

Journal of Experimental Medicine ◽

10.1084/jem.142.3.709 ◽

1975 ◽

Vol 142 (3) ◽

pp. 709-721 ◽

Cited By ~ 83

Author(s):

J Siegel ◽

A P Osmand ◽

M F Wilson ◽

H Gewurz

Keyword(s):

Inflammatory Response ◽

Potential Role ◽

Egg White ◽

Phosphate Esters ◽

C Reactive Protein ◽

Cationic Proteins ◽

Reactive Protein ◽

Naturally Occurring ◽

The Complement System

Cationic homopolymers of poly-L-lysine were found to activate complement (C) via C-reactive protein (CRP) and deplete C3 and C5 as well as early-acting C components. Maximum C consumption was obtained with polymers of 2,000-8,000 daltons; polymers of 1,700, 11,000, and 23,000 daltons were intermediate in reactivity, while L-lysine, lysyl-L-lysine, tetra-L-lysine, and polymers of 70,000-400,000 daltons lacked significant C-consuming activity. Naturally occurring polycations which consumed C in the presence of CRP included myelin basic proteins, cationic proteins of rabbit leukocytes, and both lysine- and arginine-rich histones; poly-L-arginine polymers of 17,000 but not 65,000 daltons also were C-consuming. Polycations without such reactivity included poly-L-orithine (5,000 and 165,000 daltons), egg white and human lysozymes, and Polybrene. The polycations which failed to induce C consumption via CRP, inhibited its consumption by both active polycations and by C-polysaccharide (CPS). The relative inhibitory capacity of phosphorylcholine and polycations in CPS- and polycations-CRP systems was consistent with the concept that phosphate esters and polycations react at the same or an overlapping combining site. The ability of certain polycations to activate C via CRP increases the potential for initiation of host reactions via C. The capacity of other polycations to inhibit C activation via CRP introduces a potential for physiologic or pharmacologic manipulation. These considerations would seem to expand the potential role of CRP in the initiation and modulation of the inflammatory response.

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Dietary B Vitamins and Serum C-Reactive Protein in Persons With Human Immunodeficiency Virus Infection

Food and Nutrition Bulletin ◽

10.1177/0379572116657268 ◽

2016 ◽

Vol 37 (4) ◽

pp. 517-528 ◽

Cited By ~ 5

Author(s):

Kalpana Poudel-Tandukar ◽

Ram Krishna Chandyo

Keyword(s):

Geometric Mean ◽

Latex Agglutination ◽

B Vitamins ◽

C Reactive Protein ◽

Cross Sectional Survey ◽

Cross Sectional ◽

Reactive Protein ◽

The Mean ◽

Serum Crp

Background: B vitamins may have beneficial roles in reducing inflammation; however, research on the role of B vitamins in inflammation among HIV-infected persons is lacking. Objective: This study assessed the association between B vitamins and serum C-reactive protein (CRP) concentrations in HIV-infected persons. Methods: A cross-sectional survey was conducted among 314 HIV-infected persons (180 men and 134 women) aged 18 to 60 years residing in the Kathmandu, Nepal. High-sensitive and regular serum CRP concentrations were measured by the latex agglutination nephelometry and latex agglutination turbidimetric method, respectively. Dietary intake was assessed using 2 nonconsecutive 24-hour dietary recalls. The relationships between B vitamins and serum CRP concentrations were assessed using multiple regression analysis. Results: The multivariate-adjusted geometric mean of serum CRP concentrations was significantly decreased with an increasing B vitamins intake across quartiles of niacin ( P for trend = .007), pyridoxine ( P for trend = .042), and cobalamin ( P for trend = .037) in men. In men, the mean serum CRP concentrations in the highest quartiles of niacin, pyridoxine, and cobalamin were 63%, 38%, and 58%, respectively, lower than that in the lowest quartile. In women, the mean serum CRP concentrations in the highest quartiles of riboflavin ( P for trend = .084) and pyridoxine ( P for trend = .093) were 37% and 47%, respectively, lower than that in the lowest quartile. Conclusion: High intake of niacin, pyridoxine, or cobalamin was independently associated with decreased serum CRP concentrations among HIV-infected men. Further prospective studies are warranted to confirm the role of B vitamins in inflammation among HIV-infected persons.

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Role of Doppler Ultrasonography and C-Reactive Protein in Patients with Acute Scrotum

Global Journal of Health Science ◽

10.5539/gjhs.v9n1p10 ◽

2016 ◽

Vol 9 (1) ◽

pp. 10

Author(s):

Abdulwahid G. Mohammed ◽

Bahaaedin A. Elkhader ◽

Charbel Saade ◽

Maram A. Fagiri ◽

Mustafa Z. Mahmoud

Keyword(s):

Doppler Ultrasonography ◽

Clinical Symptoms ◽

Acute Scrotum ◽

C Reactive Protein ◽

Reactive Protein ◽

Array Transducer ◽

Preoperative Diagnostic ◽

Turbidimetric Analysis ◽

Serum Crp

This research was designate with an intention to find the role of Doppler ultrasonography (DUS) and C-reactive protein (CRP) in patients with acute scrotum. A sum of 80 (mean age 23±2.1 years) consequential patients were recruited for a period of six months between July 2015 and January 2016 in this prospective study. Collected data confined age, medical history, and clinical symptoms. DUS was performed with a linear array transducer (10-15 MHz) connected to HI vision Avius ultrasound unit; Hitachi. CRP was measured using turbidimetric analysis through the Cobas Integra analyzer system (Test CRPL2, 0-293); Roche Diagnostics. Statistical analysis was accomplished using the standard Statistical Package for the Social Sciences version 20 for Windows; Microsoft. Fitted achievement values for DUS in the diagnosis of testicular torsion, epididymo-orchitis, and varicocele were respectively 100%, 91% and 88% for sensitivity, 92%, 71%, and 90% for specificity, and 94%, 83%, and 89% for accuracy. An increased in the serum CRP level (63.2±40.2 mg/L) was seen in an inflammatory cause of the acute scrotum. In contrast, there was no increase in CRP level (8±3.1 mg/L) in the non-inflammatory cases (P < 0.0001). Scrotal DUS is a highly precise preoperative diagnostic instrument, thereby confirm its course application in the incipient triage of patients with acute scrotum. Serum level of CRP can afford useful advice for differentiation between inflammatory and other reasons of the acute scrotum.

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Association Between Serum Selenium and Serum C-Reactive Protein Concentrations in Persons Infected With Human Immunodeficiency Virus Infection

Current Developments in Nutrition ◽

10.1093/cdn/nzab059_024 ◽

2021 ◽

Vol 5 (Supplement_2) ◽

pp. 1323-1323

Author(s):

Kalpana Poudel-Tandukar ◽

Krishna !Poudel

Keyword(s):

Selenium Deficiency ◽

Selenium Concentration ◽

Serum Selenium ◽

C Reactive Protein ◽

Reactive Protein ◽

Immunodeficiency Virus ◽

Serum Selenium Levels ◽

Serum Selenium Concentration ◽

Serum Crp

Abstract Objectives Chronic inflammation and selenium deficiency are common in persons infected with the human immunodeficiency virus (HIV). Both C-reactive protein, a pro-inflammatory biomarker, and selenium deficiency have been associated with accelerated disease progression and poorer survival among persons infected with HIV-infection. However, research on the role of serum selenium levels in inflammation is limited. We assessed the association between serum selenium levels and C-reactive protein (CRP) concentrations in a cohort of persons infected with HIV. Methods A cross-sectional survey was conducted among 233 HIV-infected persons (124 men and 109 women) aged 18–60 years who were residing in Kathmandu, Nepal. Regular serum CRP and selenium concentrations were measured by the latex agglutination turbidimetric and the atomic absorption method, respectively. Relationships were assessed using multiple linear regression analysis to adjust for potential confounding factors including sociodemographic, smoking, body mass index, chronic diseases, CD4+ T-cell count, and anti-retroviral therapy. Results The geometric means of serum selenium and CRP concentrations were 9.65 µg/dL and 1.43 mg/L, respectively. Mean serum CRP concentration was significantly decreased with increasing serum selenium concentration across selenium tertiles (P for trend = 0.019), with mean serum CRP concentration in the highest tertile of serum selenium concentration was 40.8% lower than that in the lowest tertile. We found a significant inverse relation between log selenium and log CRP concentrations (beta for 1 unit change in log selenium; β = −1.01, p = 0.06). Conclusions Serum selenium levels may be inversely associated with serum CRP concentrations in persons infected with HIV. Further prospective study to confirm the role of serum selenium levels in inflammation among persons infected with HIV is warranted. Funding Sources University of Massachusetts Amherst.

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Prognostic role of C-reactive protein levels in patients with hepatocellular carcinoma undergoing sorafenib therapy.

Journal of Clinical Oncology ◽

10.1200/jco.2014.32.3_suppl.340 ◽

2014 ◽

Vol 32 (3_suppl) ◽

pp. 340-340

Author(s):

Jean Kyung Lee ◽

James J. Harding ◽

Joanne F. Chou ◽

Marinela Capanu ◽

Leonard Saltz ◽

...

Keyword(s):

Time Dependent ◽

C Reactive Protein ◽

Prognostic Role ◽

Sorafenib Treatment ◽

Advanced Hcc ◽

Reactive Protein ◽

Ajcc Stage ◽

Sorafenib Therapy ◽

Serum Crp

340 Background: Markers of systemic inflammatory response including C-reactive protein (CRP) are associated with the prognosis of various malignancies including HCC. We investigated the significance of the serum CRP level as a prognostic factor in patients with advanced HCC on sorafenib therapy. Methods: The MSKCC institutional database was retrospectively queried for patients diagnosed with advanced HCC who received sorafenib therapy from 2002-2012 and had available serum CRP measurements. Patient demographics and outcomes were extracted under an IRB-approved waiver. Baseline CRP levels within 30 days of starting of sorafenib and while on sorafenib treatment were correlated with clinical outcomes. CRP levels after the initiation of sorafenib were analyzed as a time dependent covariate in a Cox regression model. Results: 51 patients had available CRP levels and received sorafenib: Median age 65 years (range 45-82); KPS 80 (70-90%); 9 females (17.6%); 32 AJCC stage IV disease (62.7%); 10 AJCC stage III (19.6%); 12 hepatitis C (23.5%), 10 hepatitis B (19.6%), 12 alcohol (23.5%), and 20 non-alcoholic steatohepatitis (39%); and 43 Child-Pugh A (84.3 %). The median time on sorafenib was 2.4 months (range 0.2-21.6). The median overall survival (OS) was 9.2 months [CI 5.8 – 13.6] and the median progression-free survival (PFS) was 2.1 months [CI 1.6 – 2.6]. 46 of 51 patients had baseline CRP levels available for analysis. Baseline CRP level was associated with OS [hazard ratio (HR) 1.2 (CI 0.996 – 1.4, p=0.056)] but not with PFS [HR 1.1 (CI 0.96-1.3, p=0.14)]. Time dependent covariate analysis demonstrated an association between CRP levels during sorafenib therapy and overall OS [HR 1.08 (CI 1.01-1.16, p=0.02)] as well as PFS [HR 1.09 (CI 1.02 – 1.16, p=0.009)]. Conclusions: Baseline CRP level prior to the start of sorafenib therapy showed a borderline significant association with OS but no association with PFS. Higher CRP levels showed a significant association with increased risk of disease progression and death for sorafenib treated HCC patients. Potential prognostic role of CRP in HCC patients undergoing sorafenib therapy is to be further studied prospectively.

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Serum C-reactive protein concentrations in dogs with idiopathic epilepsy

Vlaams Diergeneeskundig Tijdschrift ◽

10.21825/vdt.v86i2.16292 ◽

2017 ◽

Vol 86 (2) ◽

pp. 79-83

Author(s):

E. Segers ◽

V. Martlé ◽

S. Piepers ◽

L. Van Ham ◽

S. F. M. Bhatti

Keyword(s):

Acute Phase ◽

Acute Phase Proteins ◽

Idiopathic Epilepsy ◽

Control Group ◽

C Reactive Protein ◽

Inflammatory Reactions ◽

Reactive Protein ◽

Cluster Seizures ◽

Serum Crp

Inflammatory reactions in dogs are associated with systemic changes in serum, called the acute phase response; changes in the concentration of acute phase proteins in the serum take place. C-reactive protein (CRP) is a positive acute phase protein, which increases during inflammation. The role of inflammation in epilepsy remains unclear. In this study, the inflammatory response in dogs with idiopathic epilepsy (IE) was investigated. The aims of the study were: 1. to measure serum CRP concentrations in dogs with IE and in healthy dogs, 2. to measure serum CRP concentrations in dogs with acute cluster seizures and in dogs with isolated seizures and 3. to observe the evolution of serum CRP concentrations in time after the last seizure. This study showed no significant differences in serum CRP concentrations between dogs with IE (7.8 mg/l) and dogs of the control group (8.3 mg/l). Furthermore, the results showed higher mean serum CRP concentrations in dogs with IE exhibiting cluster seizures (11,8 mg/l) than in dogs with isolated seizures (5.7 mg/l). However, these results were not statistically significant (P = 0,077). Finally, no statistically significant decrease in serum CRP concentrations was seen with time after the last epileptic seizure in dogs with IE (P = 0,077).

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