Magnetically induced self-assembly DNAzyme electrochemical biosensor based on gold-modified α-Fe2O3/Fe3O4 heterogeneous nanoparticles for sensitive detection of Ni2+

2021 ◽  
Author(s):  
Lulu Yu ◽  
Min Liu ◽  
Yanling Zhang ◽  
Yun Ni ◽  
Shaobo Wu ◽  
...  
Keyword(s):  
Self Assembly ◽  
Electrochemical Biosensor ◽  
Limit Of Detection ◽  
Sol Gel ◽  
Trisodium Citrate ◽  
Thiol Group ◽  
Differential Pulse ◽  
Electrochemical Sensing ◽  
Detection Range ◽  
Sensing Platform

Abstract A magnetically induced self-assembly DNAzyme electrochemical biosensor based on gold-modified α-Fe2O3/Fe3O4 heterogeneous nanoparticles was successfully fabricated to detect Nickel(II) (Ni2+). The phase composition and magnetic properties of α-Fe2O3/Fe3O4 heterogeneous nanoparticles controllably prepared by the citric acid (CA) sol-gel method were investigated in detail. The α-Fe2O3/Fe3O4 heterogeneous nanoparticles were modified by using trisodium citrate as reducing agent, and the magnetically induced self-assembly α-Fe2O3/Fe3O4-Au nanocomposites were obtained. The designed Ni2+-dependent DNAzyme consisted of the catalytic chain modified with the thiol group (S1-SH) and the substrate chain modified with methylene blue (S2-MB). The MGCE/α-Fe2O3/Fe3O4-Au/S1/BSA/S2 electrochemical sensing platform was constructed and differential pulse voltammetry (DPV) was applied for electrochemical detection. Under the optimum experimental parameters, the detection range of the biosensor was 100 pM-10 µM (R2= 0.9978) with the limit of detection (LOD) of 55 pM. The biosensor had high selectivity, acceptable stability, and reproducibility (RSD = 4.03%).

scholarly journals Electrochemical sensing platform for simultaneous detection of 6-mercaptopurine and 6-thioguanine using RGO-Cu2O/Fe2O3 modified screen-printed graphite electrode

2021 ◽  
Author(s):  
Fatemeh Irannezhad ◽  
Jamileh Seyed-Yazdi ◽  
Seyedeh Hoda Hekmatara
Keyword(s):  
Dynamic Range ◽  
Graphite Electrode ◽  
Limit Of Detection ◽  
Simultaneous Detection ◽  
Differential Pulse ◽  
Electrochemical Sensing ◽  
X Ray Diffraction ◽  
Sensing Platform ◽  
Facile Fabrication ◽  
Screen Printed

A sensitive electrochemical sensor was developed using reduced graphene oxide RGO-Cu2O/Fe2O3 nanocomposite for 6-mercaptopurine detection based on a facile fabrication method. The surface morphology and structural composition of this nanocomposite was evaluated by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), and Fourier transform infrared (FT-IR) spectroscopy. The screen-printed graphite electrode (SPGE) modified with RGO-Cu2O/Fe2O3 nanocomposite (RGO-Cu2O/Fe2O3/SPGE) indicated excellent electrochemical properties to detect 6-mercaptopurine. The linear dynamic range was estimated between 0.05 and 400.0 μM for 6-mercaptopurine detection, with a limit of detection of 0.03 μM. Also, RGO-Cu2O/Fe2O3/SPGE sensor showed good activity for simultaneous detection of 6-mercaptopurine and 6-thioguanine. In the coexistence system of 6-mercaptopurine and 6-thioguanine, two clear and well-isolated voltammetric peaks were obtained by differential pulse voltammetry (DPV). Additionally, the proposed sensor was examined for applicability by determining 6-mercaptopurine and 6-thioguanine in real samples, and the recovery in the range of 97.5-103.0 % was obtained.

scholarly journals Simultaneous electrochemical sensing of dihydroxy benzene isomers at cost-effective allura red polymeric film modified glassy carbon electrode

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Pattan-Siddappa Ganesh ◽  
Ganesh Shimoga ◽  
Seok-Han Lee ◽  
Sang-Youn Kim ◽  
Eno E. Ebenso
Keyword(s):  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Limit Of Detection ◽  
Tap Water ◽  
Differential Pulse ◽  
Oxidation Potential ◽  
Electrochemical Sensing ◽  
Carbon Electrode ◽  
Buffer Solution ◽  
Allura Red

Abstract Background A simple and simultaneous electrochemical sensing platform was fabricated by electropolymerization of allura red on glassy carbon electrode (GCE) for the interference-free detection of dihydroxy benzene isomers. Methods The modified working electrode was characterized by electrochemical and field emission scanning electron microscopy methods. The modified electrode showed excellent electrocatalytic activity for the electrooxidation of catechol (CC) and hydroquinone (HQ) at physiological pH of 7.4 by cyclic voltammetric (CV) and differential pulse voltammetric (DPV) techniques. Results The effective split in the overlapped oxidation signal of CC and HQ was achieved in a binary mixture with peak to peak separation of 0.102 V and 0.103 V by CV and DPV techniques. The electrode kinetics was found to be adsorption-controlled. The oxidation potential directly depends on the pH of the buffer solution, and it witnessed the transfer of equal number of protons and electrons in the redox phenomenon. Conclusions The limit of detection (LOD) for CC and HQ was calculated to be 0.126 μM and 0.132 μM in the linear range of 0 to 80.0 μM and 0 to 110.0 μM, respectively, by ultra-sensitive DPV technique. The practical applicability of the proposed sensor was evaluated for tap water sample analysis, and good recovery rates were observed. Graphical abstract Electrocatalytic interaction of ALR/GCE with dihydroxy benzene isomers.

scholarly journals An efficient electrochemical sensing of hazardous catechol and hydroquinone at direct green 6 decorated carbon paste electrode

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
K. Chetankumar ◽  
B. E. Kumara Swamy ◽  
S. C. Sharma ◽  
S. A. Hariprasad
Keyword(s):  
Carbon Paste Electrode ◽  
Limit Of Detection ◽  
Tap Water ◽  
Differential Pulse ◽  
Electrochemical Sensing ◽  
Carbon Paste ◽  
Pulse Voltammetry ◽  
Two Peaks ◽  
Paste Electrode ◽  
Scanning Electron

AbstractIn this proposed work, direct green 6 (DG6) decorated carbon paste electrode (CPE) was fabricated for the efficient simultaneous and individual sensing of catechol (CA) and hydroquinone (HY). Electrochemical deeds of the CA and HY were carried out by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) at poly-DG6-modfied carbon paste electrode (Po-DG6-MCPE). Using scanning electron microscopy (SEM) studied the surface property of unmodified CPE (UCPE) and Po-DG6-MCPE. The decorated sensor displayed admirable electrocatalytic performance with fine stability, reproducibility, selectivity, low limit of detection (LLOD) for HY (0.11 μM) and CC (0.09 μM) and sensor process was originated to be adsorption-controlled phenomena. The Po-DG6-MCPE sensor exhibits well separated two peaks for HY and CA in CV and DPV analysis with potential difference of 0.098 V. Subsequently, the sensor was practically applied for the analysis in tap water and it consistent in-between for CA 93.25–100.16% and for HY 97.25–99.87% respectively.

scholarly journals Surface-Enhanced Oxidation and Determination of Isothipendyl Hydrochloride at an Electrochemical Sensing Film Constructed by Multiwalled Carbon Nanotubes

2012 ◽  
Vol 2012 ◽  
pp. 1-6
Author(s):  
S. N. Prashanth ◽  
Shankara S. Kalanur ◽  
Nagappa L. Teradal ◽  
J. Seetharamappa
Keyword(s):  
Limit Of Detection ◽  
Biological Fluids ◽  
Differential Pulse ◽  
Electrochemical Sensing ◽  
Good Precision ◽  
Multiwalled Carbon ◽  
Limit Of Quantification ◽  
Surface Enhanced ◽  
Ph Range

The electrochemical behavior of isothipendyl hydrochloride (IPH) was investigated at bare and multiwalled-carbon-nanotube modified glassy carbon electrode (MWCNT-GCE). IPH (55 μM) showed two oxidation peaks in Britton-Robinson (BR) buffer of pH 7.0. The oxidation process of IPH was observed to be irreversible over the pH range of 2.5–9.0. The influence of pH, scan rate, and concentration of the drug on anodic peak was studied. A differential pulse voltammetric method with good precision and accuracy was developed for the determination of IPH in pure and biological fluids. The peak current was found to be linearly dependent on the concentration of IPH in the range of 1.25–55 μM. The values of limit of detection and limit of quantification were noticed to be 0.284 and 0.949 μM, respectively.

Core-shell Cu@C@ZIF-8 composite: a high-performance electrode material for electrochemical sensing of nitrite with high selectivity and sensitivity

2021 ◽  
Author(s):  
Feng Gao ◽  
Xiaolong Tu ◽  
Yongfang Yu ◽  
Yansha Gao ◽  
Jin Zou ◽  
...  
Keyword(s):  
High Performance ◽  
High Selectivity ◽  
Limit Of Detection ◽  
Metal Organic Framework ◽  
High Sensitivity ◽  
Electrochemical Sensing ◽  
Core Shell ◽  
Zeolitic Imidazolate Framework ◽  
Sensing Platform ◽  
The Difference

Abstract Herein, an efficient electrochemical sensing platform is proposed for selective and sensitive detection of nitrite on the basis of Cu@C@Zeolitic imidazolate framework-8 (Cu@C@ZIF-8) heterostructure. Core-shell Cu@C@ZIF-8 composite was synthesized by pyrolysis of Cu-metal-organic framework@ZIF-8 (Cu-MOF@ZIF-8) in Ar atmosphere on account of the difference of thermal stability between Cu-MOF and ZIF-8. For the sensing system of Cu@C@ZIF-8, ZIF-8 with proper pore size allows nitrite diffuse through the shell, while big molecules cannot, which ensures high selectivity of the sensor. On the other hand, Cu@C as electrocatalyst promotes the oxidation of nitrite, thereby resulting high sensitivity of the sensor. Accordingly, the Cu@C@ZIF-8 based sensor presents excellent performance for nitrite detection, which achieves a wide linear response range of 0.1 µM to 300.0 µM, and a low limit of detection (LOD) of 0.033 µM. In addition, the Cu@C@ZIF-8 sensor possesses excellent stability and reproducibility, and was employed to quantify nitrite in sausage samples with recoveries of 95.45-104.80%.

scholarly journals An Electrochemical Sensor Based on Gold Nanodendrite/Surfactant Modified Electrode for Bisphenol A Detection

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Nguyen Thi Lien ◽  
Le Quoc Hung ◽  
Nguyen Tien Hoang ◽  
Vu Thi Thu ◽  
Dau Thi Ngoc Nga ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Electrochemical Sensors ◽  
Differential Pulse ◽  
Electrochemical Sensing ◽  
Fluorescence Measurements ◽  
Sensing Platform ◽  
Electrochemical Window ◽  
Galvanostatic Deposition ◽  
Bisphenol A Detection ◽  
Good Agreement

In the present work, we reported the simple way to fabricate an electrochemical sensing platform to detect Bisphenol A (BPA) using galvanostatic deposition of Au on a glassy carbon electrode covered by cetyltrimethylammonium bromide (CTAB). This material (CTAB) enhances the sensitivity of electrochemical sensors with respect to the detection of BPA. The electrochemical response of the modified GCE to BPA was investigated by cyclic voltammetry and differential pulse voltammetry. The results displayed a low detection limit (22 nm) and a linear range from 0.025 to 10 µm along side with high reproducibility (RSD = 4.9% for seven independent sensors). Importantly, the prepared sensors were selective enough against interferences with other pollutants in the same electrochemical window. Notably, the presented sensors have already proven their ability in detecting BPA in real plastic water drinking bottle samples with high accuracy (recovery range = 96.60%–102.82%) and it is in good agreement with fluorescence measurements.

An electrochemical sensing platform based on local repression of electrolyte diffusion for single-step, reagentless, sensitive detection of a sequence-specific DNA-binding protein

The Analyst ◽  
2014 ◽  
Vol 139 (9) ◽  
pp. 2193-2198 ◽  
Author(s):  
Yun Zhang ◽  
Fang Liu ◽  
Jinfang Nie ◽  
Fuyang Jiang ◽  
Caibin Zhou ◽  
...  
Keyword(s):  
Dna Binding ◽  
Electrode Surface ◽  
Electrochemical Biosensor ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Single Step ◽  
Dna Probe ◽  
Electrochemical Sensing ◽  
Sensing Platform ◽  
First Time

This paper describes for the first time an electrochemical biosensor, which employs a DNA probe modified with a redox tag close to electrode surface, for picomolar detection of a sequence-specific DNA-binding protein.

scholarly journals Magnetic and Mesoporous Silica-Niobia Material as Modifier of Carbon Paste Electrode for p-Nitrophenol Electrochemical Determination

2021 ◽  
Author(s):  
Oleg Tkachenko ◽  
Danielle da Rosa ◽  
Anike Virgili ◽  
Marcos Vasconcellos ◽  
Tania Costa ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Carbon Paste Electrode ◽  
Electrochemical Sensors ◽  
Limit Of Detection ◽  
Sol Gel ◽  
Synthesis Method ◽  
Differential Pulse ◽  
Carbon Paste ◽  
Wide Range ◽  
Paste Electrode

In the present work, the sol-gel synthesis method was employed as strategy to obtain a magnetic and mesoporous silica-niobia material. The planned synthesis was based on the heterocondensation of niobium and silicon alkoxide precursors, in the presence of spherical magnetite particles. The resulting material presented interesting characteristics such as magnetism, large mesopores, in the range from 20 to 50 nm, and 68 m2 g−1 of surface area. These features allowed its use as modifier of carbon paste electrode for p-nitrophenol determination, since niobia has never been used in electrochemical sensors for the determination of nitrophenol compounds. By using differential pulse voltammetry technique, the electrode can be applied in a wide range of p-nitrophenol concentration, from 10 to 490 μmol L−1, with a limit of detection of 1.2 µmol L−1 and sensitivity up to 0.60 µA L µmol−1. The proposed electrode presented good sensitivity and selectivity and it was applied in real water samples.

scholarly journals An Electrochemical Sensor based on Electrodeposited CTAB Film on Glassy Carbon Electrode for Detection of Morphine

2019 ◽  
Vol 32 (1) ◽  
pp. 137-141
Author(s):  
Pinky Abraham ◽  
S. Renjini ◽  
V. Anithakumary ◽  
P.G. Chithra
Keyword(s):  
Thin Film ◽  
Glassy Carbon ◽  
Limit Of Detection ◽  
Differential Pulse ◽  
Carbon Electrode ◽  
Sensing Platform ◽  
Excellent Electrochemical Performance ◽  
Tertiary Amino ◽  
Pulse Voltammetry ◽  
Glassy Carbon Modified Electrode

A simple, effective and rapid method for the electrochemical detection of morphine is described based on glassy carbon modified electrode with poly(CTAB). In this work, poly(CTAB) thin film was generated through elecropolymerization of the surfactant CTAB. The formation of nanoporous thin film of poly(CTAB) was confirmed by field emission scanning electron microscopy (FESEM) with energy dispersive spectra (EDS) and Fourier transform infrared spectroscopy (FTIR). The electrochemical behavior of morphine is explained in terms of the anodic oxidation of its tertiary amino group. The limit of detection was calculated as 0.2 μM with a good regression between concentration and peak current of morphine by using differential pulse voltammetry within the range of 50 nM to 20 μM. The poly(CTAB)/GCE based sensor shows excellent electrochemical performance for the detection of morphine and this sensing platform can be effective for the detection of similar molecules.

scholarly journals Comparative Studies of CPEs Modified with Distinctive Metal Nanoparticle-Decorated Electroactive Polyimide for the Detection of UA

Polymers ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 252
Author(s):  
Aamna Bibi ◽  
Sheng-Chieh Hsu ◽  
Wei-Fu Ji ◽  
Yi-Chi Cho ◽  
Karen S. Santiago ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Initial Step ◽  
Differential Pulse ◽  
Metal Nanoparticle ◽  
Carbon Paste ◽  
Detection Range ◽  
Au Nps ◽  
Linear Detection ◽  
Paste Electrode

In this present work, an electrochemical sensor was developed for the sensing of uric acid (UA). The sensor was based on a carbon paste electrode (CPE) modified with electroactive polyimide (EPI) synthesized using aniline tetramer (ACAT) decorated with reduced nanoparticles (NPs) of Au, Pt, and Ag. The initial step involved the preparation and characterization of ACAT. Subsequently, the ACAT-based EPI synthesis was performed by chemical imidization of its precursors 4,4′-(4.4′-isopropylidene-diphenoxy) bis (phthalic anhydride) BPADA and ACAT. Then, EPI was doped with distinctive particles of Ag, Pt and Au, and the doped EPIs were abbreviated as EPIS, EPIP and EPIG, respectively. Their structures were characterized by XRD, XPS, and TEM, and the electrochemical properties were determined by cyclic voltammetry and chronoamperometry. Among these evaluated sensors, EPI with Au NPs turned out the best with a sensitivity of 1.53 uA uM−1 UA, a low limit of detection (LOD) of 0.78 uM, and a linear detection range (LDR) of 5–50 uM UA at a low potential value of 310 mV. Additionally, differential pulse voltammetric (DPV) analysis showed that the EPIG sensor showed the best selectivity for a tertiary mixture of UA, dopamine (DA), and ascorbic acid (AA) as compared to EPIP and EPIS.

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