scholarly journals Antioxidant Activity Test of Chayote (Sechium edule (Jacq.) Swartz) Ethanol Extract using DPPH Method

2021 ◽  
Vol 1819 (1) ◽  
pp. 012035
Author(s):  
A S Daulay ◽  
Ridwanto ◽  
R A Syahputra ◽  
A Nafitri
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Extract ◽  
Sechium Edule ◽  
Activity Test ◽  
Dpph Method

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Daun Ganitri (Elaeocarpus Ganitrus Roxb.) dengan Metode DPPH (2,2 Difenil-1-Pikrilhidazil)

2021 ◽  
Vol 18 (1) ◽  
pp. 60-67
Author(s):  
Naelaz Zukhruf Wakhidatul Kiromah ◽  
Sadam Husein ◽  
Titi Pudji Rahayu
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Antioxidant Compounds ◽  
Synthetic Antioxidant ◽  
Activity Test ◽  
Impaired Liver ◽  
Ic50 Value ◽  
Dpph Method

Free radicals are one of the cause of various diseases. The use of synthetic antioxidant compounds could prevent the effect of the free radicals, however may cause adverse effects on the human body such as impaired liver, lung, intestinal and poisoning. Therefore antioxidant from natural resources needs to be developed. The purpose of this research was to determine the antioxidant activity and IC50 value of the ethanol extract of ganitri (Elaeocarpus ganitrus Roxbs.) leaves. Ganitri leaf ethanol extract activity test was carried out using DPPH method with vitamin C as a standard. Antioxidant activity was determined as a decreas in the absorbance of DPPH at 517 nm wavelength after an addition of the extract with the concentrations of 20, 40, 80, and 100 ppm. The antioxidant acitivity measurement of the ganitri leaf extract showed that the linier regression equation obtained was y = 0.3669x + 29.546, r = 0.4573 while the IC50 value was 54,12 ppm. Based on the result, it is concluded that the ethanol extract of ganitri (Elaeocarpus ganitrus Roxb.) leaf showed was categorized as strong antioxidant.

scholarly journals AKTIVITAS ANTIOKSIDAN EKSTRAK TUMBUHAN PAKU EKOR KUDA (Equisetum debile L.) TERHADAP PEROKSIDASI LIPID PLASMA DARAH MENCIT (Mus musculus)

2017 ◽  
Vol 4 (1) ◽  
pp. 48
Author(s):  
Riana Dyah Suryaningrum ◽  
Ni Made Puspawati ◽  
Ni Putu Adriani Astiti
Keyword(s):  
Antioxidant Activity ◽  
Statistical Analysis ◽  
Free Radical ◽  
Blood Plasma ◽  
Mus Musculus ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Free Radical Scavenging ◽  
Activity Test ◽  
Dpph Method

The purpose of this research was to study the antioxidant activity from ethanol extract of horstail (Equisetum debile L.) in the free radical scavenging in mice blood plasma. The antioxidant activity test was conducted with the DPPH method and measuring the MDA concentration in mice blood. The antioxidant activity test with the DPPH results showed that the ethanol extract of horstail (Equisetum debile L.) had the antioxidant activity of IC50 which was 1.604 mg/mL or 1,604 ppm. The statistical analysis result of the MDA blood plasma in mice with various doses (125 mg/kgBM, 250 mg/kgBM, 375 mg/kgBM and 500 mg/kgBM) showed that the 500 mg/kgBM dose extract was able to reduce the MDA concentration in mice blood which given the most amount of exercise.

scholarly journals Isolation of Flavonoid Compounds from Eceng Gondok (Eichhornia crassipes) and Antioxidant Tests with DPPH (1,1-Diphenyl-2-Picrylhydrazyl) Method

2018 ◽  
Vol 21 (4) ◽  
pp. 187-192 ◽  
Author(s):  
Kautsar Elvira ◽  
Enny Fachriyah ◽  
Dewi Kusrini
Keyword(s):  
Antioxidant Activity ◽  
Water Hyacinth ◽  
Eichhornia Crassipes ◽  
Preparative Thin Layer Chromatography ◽  
Ethanol Extract ◽  
Basic Structure ◽  
Flavonoid Compounds ◽  
Activity Test ◽  
Dpph Method ◽  
Layer Chromatography

The research had been conducted of the isolation of flavonoid compounds from water hyacinth plants (Eichhornia crassipes) and antioxidant activity test by DPPH (1,1-diphenyl-2-picrylhydrazyl) method. This research starts from maceration of water hyacinth with ethanol solvent. Ethanol extract was hydrolyzed with HCl. Flavonoid separation is carried out by column chromatography and preparative thin layer chromatography. The obtained isolates were analyzed by UV vis spectrophotometer and FTIR spectrophotometer. Antioxidant activity was carried out by DPPH method. Flavonoid isolates were obtained as heavy as 4.5 mg (0.006%). The results of the analysis of flavonoid isolates A4a with UV vis spectrophotometer appear at a wavelength of 368 nm (band I) and 260 nm (band II). The results of the analysis with FTIR shown that the isolates had functional groups O-H, aliphatic C-H, CH3, C =O, aromatic C = C, and C-O ether, it was suspected that flavonoid isolates had a basic structure of quercetin 7-methyl ether compounds. The results of antioxidant activity test of flavonoid isolates shown IC50 price of 254.66 mg / L

scholarly journals UJI AKTIVITAS ANTIOKSIDAN DAUN BENALU KOPI (Loranthus Ferrugineus Roxb.) DENGAN METODE DPPH (1,1 – Difenil -2- Pikrilhidrazil)

2019 ◽  
Vol 6 (2) ◽  
pp. 192
Author(s):  
Muammar Yulian ◽  
Safrijal Safrijal
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Secondary Metabolites ◽  
Room Temperature ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Dpph Method ◽  
Ic50 Values ◽  
X 24

The study about antioxidant activity test of coffee parasite leaves (Loranthus ferrugineus Roxb.) by 1,1-diphenyl-2-pikrilhidrazyl (DPPH) method has been done. The aim of this study was to determine the content and activity of secondary metabolites, flavonoids and antioxidant, which found in the coffee parasite leaves. Dry powder of parasite coffee leaves (Loranthus ferrugineus Roxb.) as much as 0.5 kg were macerated by 2 L of ethanol solvent at room temperature for 4 x 24 hours, then mixed and filtered. Ethanol filtrate was evaporated at 30-40°C by using a rotary evaporator to obtain the crude extract of coffee parasite leaves. The results of the phytochemical screening showed positively that the extract was containing alkaloid, flavonoids, saponins, tannins and steroid compounds.The results of the antioxidant activity test by using DPPH method was obtained that the amount of antioxidant activity of the samples of ethanol extract had a very strong antioxidant activity against radical DPPH 0.05 mM, with IC50 values was obtained 6.063 ppm. Whereas, for comparison of ascorbic acid was about 3.127 ppm.

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Temu Blenyeh (Curcuma Purpurascens Blumae) Dengan Metode Dpph (1,1 Diphenyl-2-Picrylhydrazyl)

2021 ◽  
Vol 1 ◽  
pp. 29-37
Author(s):  
Oktariani Pramiastuti ◽  
Fiqih Kartika Murti ◽  
Sri Mulyati ◽  
Ulfatun Khasanah ◽  
Rima Harsa Atqiya Alquraisi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Essential Oil ◽  
Skin Infection ◽  
Ethanol Extract ◽  
Antioxidants Activity ◽  
Activity Test ◽  
Dpph Method ◽  
Anti Cancer ◽  
Oxidation Effect

ABSTRACTAntioxidants have a function to scavenge and neutralize oxidation effect caused by free radicals by preventing the formation of radicals. Temu blenyeh (Curcuma purpurascens Bl) is one of the curcuma species which is still under-researched. Traditionally, temu blenyeh is used to treat stomachache, cough, itch, and skin infection. Some studies show that temu blenyeh extract had antioxidants activity and anti-cancer. The extract contains flavonoid, terpenoid, steroid, triterpenoid, essential oil. The study aimed to determine antioxidant activity of ethanol extract of temu blenyeh using DPPH method. Temu blenyeh was extracted by maceration with 96% ethanol solvent. The antioxidants activity test described that the value of IC50 was 48.697 ppm. Keywords: Temu blenyeh, DPPH, antioxidants Abstrak Antioksidan berfungsi untuk menangkal dan menetralisasi efek oksidasi yang disebabkan radikal bebas dengan cara mencegah terbentuknya radikal. Temu blenyeh (Curcuma purpurascens Bl) salah satu spesies curcuma yang masih sedikit diteliti. Temu blenyeh secara tradisional digunakan untuk mengobati sakit perut, batuk, gatal, dan infeksi kulit. Beberapa penelitian membuktikan bahwa ekstrak rimpang temu blenyeh memiliki aktivitas antioksidan dan anti kanker. Temu blenyeh mengandung flavonoid, terpenoid, steroid, triterpenoid dan minyak esensial. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antioksidan pada ekstrak etanol temu blenyeh menggunakan metode DPPH. Temu blenyeh diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Dalam penelitian uji aktivitas antioksidan ekstrak etanol temu blenyeh menunjukkan hasil nilai IC50 sebesar 48,697 ppm. Kata kunci : Temu blenyeh; DPPH; Antioksidan

scholarly journals FORMULASI DAN UJI EFEKTIVITAS ANTIOKSIDAN SEDIAAN KRIM EKSTRAK ETANOL DAUN SALAM (Syzygium Polyanthum (Wight) Walpers.) DENGAN MENGGUNAKAN METODE DPPH (1,1-diphenyl-2-picrylhydrazyl)

PHARMACON ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 720
Author(s):  
Etserlisa A. Apitalau ◽  
Hosea Jaya Edy ◽  
Karlah L.R. Mansauda
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethanol Extract ◽  
Experimental Methods ◽  
Physical Test ◽  
A Value ◽  
Activity Test ◽  
Test Parameters ◽  
Dpph Method ◽  
The Impact

ABSTRACTBay leaf (Syzygium Polyanthum (Wight) Walpers.) is known contains flavonoids, saponins, tannins and vitamin C. Flavonoids and vitamin C have an antioxidant activity, since they have the ability to remove and effectively reduce a damaging oxidizer species. The aim of the study is to determine the impact of  the bay leaf ethanol extract enhancement concentration towards  an antioxidant activity of cream preparation by using the DPPH method. This research used laboratory experimental methods. Cream formulations are made with various concentrations 1%, 3%, 6% and 9%. The evaluation results of the bay leaf cream preparation has fulfilled the requirements of homogeneity, organoleptic, pH, adhesion, dispersion, and centrifugation. The results of the antioxidant activity test using the DPPH method using the UV-Vis spectrophotometer which was the most effective as an antioxidant cream from bay leaf extract was 3% with a value IC_50 = 1.4630 ppm and vitamin C as a comparison with a value IC_50 = 0.1131 ppm. From this research, it can be concluded that the cream formulation of bay leaf ethanol extract has fulfilled the physical test parameters, stabile, and had a powerful antioxidant activity.Keywords: : Bay Leaf (Sysigium Polyanthum (Wight) Walpers.), Antioxidant Cream, DPPH (1.1-Diphenyl-2-Picrylhydrazyl) Method  ABSTRAKDaun salam (Syzygium Polyanthum (Wight) Walpers.) diketahui mengandung flavonoid, saponin, tanin dan vitamin C. Senyawa flavonoid dan vitamin C memiliki aktivitas sebagai antioksidan, karena memiliki kemampuan untuk menghilangkan dan secara efektif mengurangi spesies pengoksidasi yang merusak. Penilitian ini bertujuan untuk mengetahui pengaruh peningkatan kosentrasi ekstrak etanol daun salam terhadap aktivitas antioksidan pada sediaan krim dengan menggunakan metode DPPH. Penelitian ini mengggunakan metode eksperimental laboratorium. Formulasi sediaan krim dibuat dengan variasi konsentrasi 1%, 3%, 6% dan 9%. Hasil evaluasi sediaan krim daun salam memenuhi persyaratan homogenitas, organoleptis, pH , daya lekat, daya sebar, sentrifugasi. Hasil uji aktivitas antioksidan dengan metode DPPH menggunakan spektrofotometer UV-Vis yang paling efektif sebagai krim antioksidan dari ekstrak daun salam adalah 3% memiliki nilai = 1,4630 ppm dan vitamin C sebagai pembanding memiliki nilai = 0,1131 ppm. Dari penilitian ini dapat disimpulkan bahwa sediaan krim ekstrak etanol daun salam memenuhi parameter uji fisik, stabil dan memiliki aktivitas antioksidan yang sangat kuat. Kata kunci : Daun Salam (Sysigium Polyanthum (Wight) Walpers.) Krim Antioksidan, Metode DPPH (1.1-Diphenyl-2-Picrylhydrazyl)

scholarly journals UJI AKTIVITAS ANTIOKSIDAN DAUN GULMA SIAM (Chromoleana odorata L.) DENGAN METODE 1,1-DIFENIL-2-PIKRILHIDRAZIL

2017 ◽  
Vol 1 (2) ◽  
pp. 131-142
Author(s):  
Ajmi Saputra ◽  
Abdul Gani ◽  
Erlidawati Erlidawati
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ethanol Extract ◽  
Complete Separation ◽  
Phytochemical Screening ◽  
Inhibitor Concentration ◽  
Methanol Fraction ◽  
Activity Test ◽  
Dpph Method ◽  
Function Group

Abstrak. Telah dilakukan penelitian uji aktivitas antioksidan daun gulma siam (Chromoleana odorata L.) dengan metode 1,1-difenil-2-pikrilhidrazil (DPPH). Tujuan penelitian ini untuk mengetahui kandungan senyawa metabolit sekunder, golongan flavonoid dan aktivitas antioksidan yang terdapat dalam daun gulma siam. Sebanyak 315 g daun gulma siam kering yang sudah dihaluskan, dimaserasi dengan etanol 95% diperoleh 100 g ekstrak kental etanol. Sebanyak 70 g ekstrak kental etanol difraksinasi dengan pelarut metanol dan n-heksana menghasilkan fraksi kental metanol 30,72 g dan n-heksana 14,62 g. Hasil skrining fitokimia ekstrak etanol menunjukkan positif mengandung senyawa alkaloid, flavonoid, saponin, tanin dan steroid. Fraksi metanol positif mengandung senyawa alkaloid, flavonoid, saponin, dan tanin. Fraksi metanol dilakukan isolasi dengan metode kromatografi kolom menggunakan eluen n-heksana : etil asetat : diklorometana (1:1:1) sebagai fase gerak pertama, kemudian ditingkatkan gradien kepolarannya untuk menghasilkan pemisahan secara sempurna. Hasil isolasi diperoleh 8 fraksi (A, B, C, D, E, F, G, H) dan hasil analisis secara KLT menunjukkan fraksi A dan B positif mengandung senyawa flavonoid. Senyawa flavonoid yang terkandung dalam fraksi A diperkirakan golongan isoflavon dan fraksi B diperkirakan golongan isoflavon, flavon, flavonol dan kalkon. Analisis FTIR menunjukkan fraksi A dan B mengandung gugus fungsi N-H, O-H (asam karboksilat), C=O (aldehid dan ester), C=C, C-H, C-X (flourida), C-O dan C-N. Hasil pengujian aktivitas antioksidan menggunakan metode DPPH, diperoleh Inhibitor concentrasi (IC50) untuk ekstrak etanol 15,5067 ppm, fraksi metanol 9,5671 ppm, fraksi A 82,7808 ppm dan fraksi B 16,2336 ppm, sedangkan untuk pembanding (asam askorbat) 0,8913 ppm. Dengan demikian, dapat disimpulkan aktivitas antioksidan yang paling kuat terdapat pada  fraksi metanol.Kata kunci: daun gulma siam, Chromoleana odorata L., flavonoid, DPPH. Abstract. The antioxidant activity of siam weed leaf (Chromoleana odorata L.) has been done with 1,1-diphenyl-2-picrilhidrazil (DPPH) method. This study is intended to know the the content of secondary metabolite compounds, the group of flavonoid and antioxidant activity that found in siam weed leaf. The 315 g dry siam weed leaf that was refined, macerated with ethanol 96% produced 100 g of ethanol viscous extract. The 70 of ethanol viscous extract was fractionated with methanol and n-hexane solvent produced 30,72 g of methanol viscous fraction and 14,62 g n-hexane. The results of phytochemical screening of ethanol extract showed positive that contained compound of alkaloids, flavonoids, saponins, tannins and steroids. Methanol fraction showed positive that contained compound of alkaloids, flavonoids, saponins and tannins. The isolation in methanol fraction was made by coloumn chomatography method using eluent n-hexene : acetate ethyl : dichloromethane (1:1:1) as first mation phase. Then the polarity gradient was increased to produce complete separation. The result of isolation produced 8 fractions (A, B, C, D, E, F, G, H) and the analysis results with TLC showed fraction of A and B was positive contain flavonoids compound. Flaconoids compound that was contained in fraction A was estimated isoflvone group and fraction B was estimated group os isoflavone, flavone, flavonol and chalcone. Analysis FTIR showed that the fraction of A and B containing function group N-H, O-H (carboxylic acid), C=O (aldehyde and ester), C=C, C-H, C-X (flouride), C-O and  C-N. The result of antioxidant activity test used DPPH method, obtained inhibitor concentration (IC50) for 15,5067 ppm of ethanol extract, 9,5671 of methanol fraction, 82,7808 ppm of fraction A and 16,2336 ppm of fraction B, while for comparison (ascorbic acid) is 0,8913 ppm. This, it can be concluded that strongert antioxidant activity was found in methanol fraction.Keywords: siam weed leaf, Chromoleana odorata L., DPPH.

scholarly journals Phytochemical Screening and Antioxidant Activity of Ethanolic Extract of Cawat Hanoman Stem (Bauhinia aculeata L.) using DPPH Method

2020 ◽  
Vol 3 (1) ◽  
pp. 15-21
Author(s):  
Rahmi Muthia ◽  
Muhammad Hidayatullah ◽  
Rahmi Hidayati
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Dpph Method ◽  
Layer Chromatography ◽  
Unstable Molecule

The free radical is an unstable molecule because contains one or two unpaired electrons. The antioxidant substance is a simple way to decrease the illness caused by free radicals. Cawat hanoman (Bauhinia aculeata L.) was known to contain tannin components one of the benefits as an antioxidant. This research aims to determine the antioxidant activity of the B. aculeata stem tested by qualitatively used thin-layer chromatography (TLC) and quantitatively using the DPPH method. Bauhinia aculeata stem was extracted using a maceration extract method with 96% ethanol. Antioxidant activity test was done qualitatively by eluent of ethyl acetate : methanol : purified water (6 : 2 : 1) using TLC and quantitatively using the DPPH method. The result of antioxidant activity from 96% ethanol extract of B. aculeata stem qualitatively showed the presence of yellow spots on a purple background at TLC after syringed DPPH 0.5 mM and quantitative test that resulted in an IC50 of 21.862 �g/mL. These results indicate that 96% ethanol extract of B. aculeata has very strong antioxidant activity.

scholarly journals Phytochemicals and GC-MS of Aquilaria beccariana van Tiegh leaves growing naturally and cultivated in North Sumatera

2021 ◽  
Vol 912 (1) ◽  
pp. 012079
Author(s):  
R Batubara ◽  
W Pulungan ◽  
T I Hanum ◽  
O Affandi
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Inhibitory Concentration ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Antioxidant Potential ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Dpph Method

Abstract Aquilaria beccariana van Tiegh is one of the agarwood (gaharu) species that grows naturally and cultivatived. However, the chemical and antioxidant potential of its leaves have not been studied much. This research aimed to examine the chemical compounds and the antioxidant activity of the ethanol extract of the agarwood leaves A. beccariana van Tiegh which grows naturally and cultivatived. The leaves were processed into simplicia and then extracted using 96% ethanol as solvent. Phytochemical screening agarwood leaves to determine the chemical compounds such as of alkaloids, flavonoids, glycosides, steroids/triterpenoids, saponins and tannis. The antioxidant activity test was carried out using the DPPH method to obtain the IC50 (Inhibitory Concentration) value. The compound tracing used GC-MS. The results showed that the simplicia and the extract of the leaves both natural-grown and cultivated contained secondary metabolites of alkaloids, flavonoids, steroids/triterpenoids, and tannins positively, whereas the glycosides were only found in cultivated type. The result of the antioxidant activity test showed that the ethanol extract of the leaves of A. beccariana van Tiegh both natural-grown and cultivated was categorized as very strong. The results of the identification of chemical compounds by GC-MS were identified 18 compounds in the natural-grown leaves, while 40 compounds were identified in the cultivated ones.

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