scholarly journals Aktivitas Antioksidan Ekstrak Etanol Temu Blenyeh (Curcuma Purpurascens Blumae) Dengan Metode Dpph (1,1 Diphenyl-2-Picrylhydrazyl)

2021 ◽  
Vol 1 ◽  
pp. 29-37
Author(s):  
Oktariani Pramiastuti ◽  
Fiqih Kartika Murti ◽  
Sri Mulyati ◽  
Ulfatun Khasanah ◽  
Rima Harsa Atqiya Alquraisi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Essential Oil ◽  
Skin Infection ◽  
Ethanol Extract ◽  
Antioxidants Activity ◽  
Activity Test ◽  
Dpph Method ◽  
Anti Cancer ◽  
Oxidation Effect

ABSTRACTAntioxidants have a function to scavenge and neutralize oxidation effect caused by free radicals by preventing the formation of radicals. Temu blenyeh (Curcuma purpurascens Bl) is one of the curcuma species which is still under-researched. Traditionally, temu blenyeh is used to treat stomachache, cough, itch, and skin infection. Some studies show that temu blenyeh extract had antioxidants activity and anti-cancer. The extract contains flavonoid, terpenoid, steroid, triterpenoid, essential oil. The study aimed to determine antioxidant activity of ethanol extract of temu blenyeh using DPPH method. Temu blenyeh was extracted by maceration with 96% ethanol solvent. The antioxidants activity test described that the value of IC50 was 48.697 ppm. Keywords: Temu blenyeh, DPPH, antioxidants Abstrak Antioksidan berfungsi untuk menangkal dan menetralisasi efek oksidasi yang disebabkan radikal bebas dengan cara mencegah terbentuknya radikal. Temu blenyeh (Curcuma purpurascens Bl) salah satu spesies curcuma yang masih sedikit diteliti. Temu blenyeh secara tradisional digunakan untuk mengobati sakit perut, batuk, gatal, dan infeksi kulit. Beberapa penelitian membuktikan bahwa ekstrak rimpang temu blenyeh memiliki aktivitas antioksidan dan anti kanker. Temu blenyeh mengandung flavonoid, terpenoid, steroid, triterpenoid dan minyak esensial. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antioksidan pada ekstrak etanol temu blenyeh menggunakan metode DPPH. Temu blenyeh diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Dalam penelitian uji aktivitas antioksidan ekstrak etanol temu blenyeh menunjukkan hasil nilai IC50 sebesar 48,697 ppm. Kata kunci : Temu blenyeh; DPPH; Antioksidan

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Daun Ganitri (Elaeocarpus Ganitrus Roxb.) dengan Metode DPPH (2,2 Difenil-1-Pikrilhidazil)

2021 ◽  
Vol 18 (1) ◽  
pp. 60-67
Author(s):  
Naelaz Zukhruf Wakhidatul Kiromah ◽  
Sadam Husein ◽  
Titi Pudji Rahayu
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Antioxidant Compounds ◽  
Synthetic Antioxidant ◽  
Activity Test ◽  
Impaired Liver ◽  
Ic50 Value ◽  
Dpph Method

Free radicals are one of the cause of various diseases. The use of synthetic antioxidant compounds could prevent the effect of the free radicals, however may cause adverse effects on the human body such as impaired liver, lung, intestinal and poisoning. Therefore antioxidant from natural resources needs to be developed. The purpose of this research was to determine the antioxidant activity and IC50 value of the ethanol extract of ganitri (Elaeocarpus ganitrus Roxbs.) leaves. Ganitri leaf ethanol extract activity test was carried out using DPPH method with vitamin C as a standard. Antioxidant activity was determined as a decreas in the absorbance of DPPH at 517 nm wavelength after an addition of the extract with the concentrations of 20, 40, 80, and 100 ppm. The antioxidant acitivity measurement of the ganitri leaf extract showed that the linier regression equation obtained was y = 0.3669x + 29.546, r = 0.4573 while the IC50 value was 54,12 ppm. Based on the result, it is concluded that the ethanol extract of ganitri (Elaeocarpus ganitrus Roxb.) leaf showed was categorized as strong antioxidant.

scholarly journals Phytochemical Screening and Antioxidant Activity of Ethanolic Extract of Cawat Hanoman Stem (Bauhinia aculeata L.) using DPPH Method

2020 ◽  
Vol 3 (1) ◽  
pp. 15-21
Author(s):  
Rahmi Muthia ◽  
Muhammad Hidayatullah ◽  
Rahmi Hidayati
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Dpph Method ◽  
Layer Chromatography ◽  
Unstable Molecule

The free radical is an unstable molecule because contains one or two unpaired electrons. The antioxidant substance is a simple way to decrease the illness caused by free radicals. Cawat hanoman (Bauhinia aculeata L.) was known to contain tannin components one of the benefits as an antioxidant. This research aims to determine the antioxidant activity of the B. aculeata stem tested by qualitatively used thin-layer chromatography (TLC) and quantitatively using the DPPH method. Bauhinia aculeata stem was extracted using a maceration extract method with 96% ethanol. Antioxidant activity test was done qualitatively by eluent of ethyl acetate : methanol : purified water (6 : 2 : 1) using TLC and quantitatively using the DPPH method. The result of antioxidant activity from 96% ethanol extract of B. aculeata stem qualitatively showed the presence of yellow spots on a purple background at TLC after syringed DPPH 0.5 mM and quantitative test that resulted in an IC50 of 21.862 �g/mL. These results indicate that 96% ethanol extract of B. aculeata has very strong antioxidant activity.

scholarly journals AKTIVITAS ANTIOKSIDAN KOMBINASI EKSTRAK ETANOL LIDAH BUAYA (Aloe vera L.) dan GANGGANG HIJAU (Ulva lactuca L.)

2021 ◽  
Vol 4 (1) ◽  
pp. 11
Author(s):  
Azizah Nada Septiawan ◽  
Emelda Emelda ◽  
Saddam Husein
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Green Algae ◽  
Aloe Vera ◽  
Ethanol Extract ◽  
Natural Antioxidants ◽  
Positive Control ◽  
Ulva Lactuca ◽  
Activity Test ◽  
Dpph Method

<h1>Abstrak</h1><p>Radikal bebas adalah atom atau molekul yang mempunyai satu atau lebih elektron tetapi tidak mempunyai pasangan, agar mempunyai pasangan radikal bebas akan berikatan dengan elektron yang ada di sekitar dengan cara menyerang elektron tersebut. Antioksidan adalah suatu zat yang dapat menetralisir atau menyerap radikal bebas. Antioksidan berdasarkan sumbernya terbagi menjadi dua yaitu antioksidan alami dan antioksidan sintesis. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan pada kombinasi ekstrak etanol lidah buaya (<em>Aloe vera </em>L.) dan ganggang hijau (<em>Ulva lactuca </em>L.) dan nilai IC<sub>50</sub> pada kombinasi ekstrak etanol lidah buaya (<em>Aloe vera</em> L.) dan ganggang hijau (<em>Ulva lactuca </em>L.)</p><p>Jenis penelitian ini adalah eksperimental untuk mengetahui aktivitas antioksidan pada ekstrak tunggal dan kombinasi ekstrak lidah buaya (<em>Aloe vera </em>L.) dan ganggang hijau (<em>Ulva lactuca </em>L.) dengan perbandingan 1:1; 1:2 dan 2:1 dengan menggunakan metode DPPH. Hasil Pengujian aktivitas antioksidan dengan metode DPPH menunjukkan bahwa kontrol positif, ekstrak etanol lidah buaya tunggal, ganggang hijau tunggal berserta kombinasi dengan perbandingan 1:1; 1:2 dan 2:1 memiliki aktivitas antioksidan kuat sampai sangat kuat. Kesimpulan dari penelitian ini adalah kombinasi ekstrak etanol lidah buaya dan ganggang hijau 1:2 memiliki potensi antioksidan tertinggi dengan nilai IC<sub>50</sub> sebesar 16,51 µg/ml.</p><p><strong>Kata kunci:</strong> <em>Aloe vera</em>;<em> </em>Antioksidan;<em>  DPPH</em>;<em>  Ulva lactuca</em></p><p><em><br /></em></p><h1>Abstract<em></em></h1><p><em>Free</em><em> radicals are atoms or molecules that have one or more electrons but do not have a pair, so that they have a pair of free radicals that will bind to the electrons around them by attacking the electrons. Antioxidants are substances that can neutralize or absorb free radicals. Antioxidants based on the source are divided into two, namely natural antioxidants and synthetic antioxidants. This research aims to determine the antioxidant activity of the combination of aloe vera (Aloe vera L.) ethanol extract and green algae (Ulva lactuca L.) and the IC<sub>50</sub> value in the combination of aloe vera (Aloe vera L.) ethanol extract and green algae (Ulva lactuca L.) extract.</em></p><p><em>This type of research is experimental to determine the antioxidant activity of single and combined extracts of aloe vera (Aloe vera L.) and green algae (Ulva lactuca L.) with a ratio of 1:1; 1:2 and 2:1 using the DPPH method. The results of the antioxidant activity test using the DPPH method showed that the positive control, ethanol extract of single aloe vera, single green algae and their combination 1:1; 1:2; 2:1 had strong to very strong antioxidant activity.<strong> </strong>The combination of ethanol extract of aloe vera and green algae 1:2 has the highest antioxidant potential with an IC<sub>50 </sub>value of 16.51 µg/ml.</em></p><p><strong><em>Key words:</em></strong><em> Aloe vera</em>;<em> </em><em> Antioxidants</em>;<em> </em><em> DPPH</em>;<em>  Ulva lactuca</em><em></em></p><p><em><br /></em></p>

scholarly journals UJI AKTIVITAS ANTIOKSIDAN SPONS Stylissa sp. DENGAN MENGGUNAKAN METODE DPPH (1,1-difenil-2-pikrilhidrazil)

PHARMACON ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 419
Author(s):  
Samuel I.M. Sibarani ◽  
Adithya Yudistira ◽  
Deby A. Mpila
Keyword(s):  
Antioxidant Activity ◽  
Cell Wall ◽  
Free Radicals ◽  
Ethanol Extract ◽  
Dna Bases ◽  
Oxidation Reactions ◽  
Terrestrial Plants ◽  
Dpph Method ◽  
High Antioxidant Activity ◽  
Ethanol Extracts

ABSTRACTHabitat of sponge Stylissa sp., were under the sea and these sponges contain active compounds, which are more active than the compounds produced by terrestrial plants. Antioxidants are inhibitors of oxidation reactions due to the free radicals, which can cause weak damage to unsaturated cells, cell wall membranes, blood vessels, DNA bases, and lipid tissue, that causing disease. The study was conducted to determine the antioxidant activity of ethanol extracts  of sponge Stylissa sp., which is located on the Bangka Island, Likupang District, North Minahasa Regency. Sponge Stylissa sp., was extracted by maceration with using ethanol. Testing of antioxidant activity was carried out by the DPPH method measured by a UV-Vis spectrophotometer. The results of the study showed that ethanol extracts of sponge Stylissa sp., has antioxidant activity in each concentration and the highest at a concentration of 100 mg / L. The conclusion is a ethanol extract of sponge Stylissa sp. have high antioxidant activity with a concentration of 25 mg/L (77,40%), concentration of 50 mg/L (85,63%), concentration of 75 mg/L (88,66%), and concentration 100 mg/L (88,96%). Key words: Stylissa sp. Sponge, Antioxidant, DPPH (1,1-diphenyl-2-picrylhydrazyl) ABSTRAKHabitat dari spons Stylissa sp. terdapat di bawah laut dan spons ini mengandung senyawa aktif yang persentase keaktifannya lebih besar dibandingkan dengan senyawa-senyawa yang dihasilkan oleh tumbuhan darat. Antioksidan adalah zat penghambat reaksi oksidasi akibat radikal bebas yang dapat menyebabkan kerusakan lemah tak jenuh, membran dinding sel, pembuluh darah, basa DNA, dan jaringan lipid sehingga menimbulkan penyakit. Penelitian dilakukan untuk mengetahui aktivitas antioksidan ekstrak etanol biota laut spons Stylissa sp. yang terdapat di pulau Bangka, Kecamatan Likupang, Kabupaten Minahasa Utara. Spons Stylissa sp. ini diekstrak dengan metode maserasi menggunakan etanol. Pengujian aktivitas antioksidan ini dilakukan dengan metode DPPH yang diukur dengan alat spektrofotometer UV-Vis. Hasil dari penelitian menunjukan bahwa ekstrak etanol spons Stylissa sp. memiliki aktivitas antioksidan disetiap konsentrasi dan yang tertinggi pada konsentrasi 100 mg/L. Kesimpulan yang didapat bahwa ekstrak etanol spons Stylissa sp. memiliki aktivitas antioksidan yang tinggi dengan konsentrasi 25 mg/L (77,40%), konsentrasi 50 mg/L (85,63%), konsentrasi 75 mg/L (88,66%), dan konsentrasi 100 mg/L (88,96%). Kata Kunci : Spons Stylissa sp., Antioksidan, DPPH (1,1-difenil-2-pikrilhidrazil)

scholarly journals AKTIVITAS ANTIOKSIDAN EKSTRAK TUMBUHAN PAKU EKOR KUDA (Equisetum debile L.) TERHADAP PEROKSIDASI LIPID PLASMA DARAH MENCIT (Mus musculus)

2017 ◽  
Vol 4 (1) ◽  
pp. 48
Author(s):  
Riana Dyah Suryaningrum ◽  
Ni Made Puspawati ◽  
Ni Putu Adriani Astiti
Keyword(s):  
Antioxidant Activity ◽  
Statistical Analysis ◽  
Free Radical ◽  
Blood Plasma ◽  
Mus Musculus ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Free Radical Scavenging ◽  
Activity Test ◽  
Dpph Method

The purpose of this research was to study the antioxidant activity from ethanol extract of horstail (Equisetum debile L.) in the free radical scavenging in mice blood plasma. The antioxidant activity test was conducted with the DPPH method and measuring the MDA concentration in mice blood. The antioxidant activity test with the DPPH results showed that the ethanol extract of horstail (Equisetum debile L.) had the antioxidant activity of IC50 which was 1.604 mg/mL or 1,604 ppm. The statistical analysis result of the MDA blood plasma in mice with various doses (125 mg/kgBM, 250 mg/kgBM, 375 mg/kgBM and 500 mg/kgBM) showed that the 500 mg/kgBM dose extract was able to reduce the MDA concentration in mice blood which given the most amount of exercise.

scholarly journals Isolation of Flavonoid Compounds from Eceng Gondok (Eichhornia crassipes) and Antioxidant Tests with DPPH (1,1-Diphenyl-2-Picrylhydrazyl) Method

2018 ◽  
Vol 21 (4) ◽  
pp. 187-192 ◽  
Author(s):  
Kautsar Elvira ◽  
Enny Fachriyah ◽  
Dewi Kusrini
Keyword(s):  
Antioxidant Activity ◽  
Water Hyacinth ◽  
Eichhornia Crassipes ◽  
Preparative Thin Layer Chromatography ◽  
Ethanol Extract ◽  
Basic Structure ◽  
Flavonoid Compounds ◽  
Activity Test ◽  
Dpph Method ◽  
Layer Chromatography

The research had been conducted of the isolation of flavonoid compounds from water hyacinth plants (Eichhornia crassipes) and antioxidant activity test by DPPH (1,1-diphenyl-2-picrylhydrazyl) method. This research starts from maceration of water hyacinth with ethanol solvent. Ethanol extract was hydrolyzed with HCl. Flavonoid separation is carried out by column chromatography and preparative thin layer chromatography. The obtained isolates were analyzed by UV vis spectrophotometer and FTIR spectrophotometer. Antioxidant activity was carried out by DPPH method. Flavonoid isolates were obtained as heavy as 4.5 mg (0.006%). The results of the analysis of flavonoid isolates A4a with UV vis spectrophotometer appear at a wavelength of 368 nm (band I) and 260 nm (band II). The results of the analysis with FTIR shown that the isolates had functional groups O-H, aliphatic C-H, CH3, C =O, aromatic C = C, and C-O ether, it was suspected that flavonoid isolates had a basic structure of quercetin 7-methyl ether compounds. The results of antioxidant activity test of flavonoid isolates shown IC50 price of 254.66 mg / L

scholarly journals Antioxidant Activity of Secondary Metabolite Compounds from Lichen Teloschistes flavicans

2021 ◽  
Vol 11 (6) ◽  
pp. 13878-13884
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Scientific Development ◽  
Molecular Formula ◽  
Separation And Purification ◽  
Specific Chemical ◽  
Antioxidants Activity ◽  
Activity Test ◽  
Dpph Method ◽  
Ic50 Values

In the last decade, lichen has become a research interest related to exploring secondary metabolites compounds. Lichen is a unique organism and contains specific chemical compounds rarely found in other natural sources; one species is the lichen Teloschistes flavicans. The objective study explores the toxicity effects and antioxidants activity properties from lichen Teloschistes flavicans isolate. The isolation of secondary metabolites was carried out by extraction, separation, and purification by means of gravity column chromatography, thin-layer chromatography, and purity tests. Toxicity activity test used the Mayer method by looking at the LC50 value, while the antioxidant activity test used the DPPH method by looking at the IC50 value. The results of the isolation obtained a needle-shaped crystal with the name of the compound is 3-[1'-(2",3"-dihydroxy-phenyl)-propyl]-7-hydroxy-chroman-4-one and the molecular formula C18H18O5. The toxicity test showed that extract and isolate compounds have LC50 values of 9.38 and 162.18 µg/mL, indicating that the isolated compound was toxic. The antioxidant test showed that the extract and isolated compounds have antioxidant activity with IC50 values of 54.05 and 127.38. The results of these studies provide scientific development of natural compounds that have the potential to act as antioxidants for diseases in the human body.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN DAUN BENALU KOPI (Loranthus Ferrugineus Roxb.) DENGAN METODE DPPH (1,1 – Difenil -2- Pikrilhidrazil)

2019 ◽  
Vol 6 (2) ◽  
pp. 192
Author(s):  
Muammar Yulian ◽  
Safrijal Safrijal
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Secondary Metabolites ◽  
Room Temperature ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Dpph Method ◽  
Ic50 Values ◽  
X 24

The study about antioxidant activity test of coffee parasite leaves (Loranthus ferrugineus Roxb.) by 1,1-diphenyl-2-pikrilhidrazyl (DPPH) method has been done. The aim of this study was to determine the content and activity of secondary metabolites, flavonoids and antioxidant, which found in the coffee parasite leaves. Dry powder of parasite coffee leaves (Loranthus ferrugineus Roxb.) as much as 0.5 kg were macerated by 2 L of ethanol solvent at room temperature for 4 x 24 hours, then mixed and filtered. Ethanol filtrate was evaporated at 30-40°C by using a rotary evaporator to obtain the crude extract of coffee parasite leaves. The results of the phytochemical screening showed positively that the extract was containing alkaloid, flavonoids, saponins, tannins and steroid compounds.The results of the antioxidant activity test by using DPPH method was obtained that the amount of antioxidant activity of the samples of ethanol extract had a very strong antioxidant activity against radical DPPH 0.05 mM, with IC50 values was obtained 6.063 ppm. Whereas, for comparison of ascorbic acid was about 3.127 ppm.

scholarly journals Antioxidant activity from ethanol extract of red seaweed (Galaxaura rugosa)

2021 ◽  
Vol 11 (2) ◽  
pp. 79
Author(s):  
Bhayu Gita Bhernama ◽  
Witri Maulidy Ayu ◽  
Cut Nuzlia
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Vitamin C ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Red Seaweed ◽  
Oxidation Reactions ◽  
Ic50 Value ◽  
Dpph Method

Antioxidants are compounds that can delay, reduce, slow down or inhibit oxidation reactions from free radical reactions. Antioxidants donate electrons to unstable free radicals so that these free radicals can be neutralized to not interfere with the body's metabolic processes. Red seaweed Galaxaura rugosa has potential as an antioxidant. The study aimed to determine the antioxidant activity of the ethanol extract of red seaweed Galaxaura rugosa against DPPH free radicals based on the IC50 value. Phytochemical testing and determination of antioxidant activity were carried out using the DPPH method (2,2-diphenyl-1-picrylhydrazyl), which was carried out quantitatively using a UV-Vis spectrophotometer. The results of the phytochemical screening of red seaweed ethanol extract contained alkaloids, flavonoids, saponins, steroids, and polyphenols and the IC50 value of red seaweed ethanol extract was 4.59 ppm, while the positive control for Vitamin C was 6.64 ppm. It was concluded that the ethanolic extract of red seaweed Galaxaura rugosa in the South Aceh District had high antioxidant potential, as evidenced by the small IC50 value of <50 µg/mL.Keywords: Antioxidants, Galauxara rugosa, phytochemicals, DPPH  ABSTRAKAktivitas antioksidan dari ekstrak etanol rumput laut merah Galaxaura rugosa Antioksidan merupakan senyawa yang mampu menunda, memperkecil, memperlambat atau menghambat reaksi oksidasi dari reaksi radikal bebas. Antioksidan menyumbangkan elektron kepada radikal bebas yang tidak stabil sehingga radikal bebas ini dapat dinetralkan agar tidak mengganggu jalannya proses metabolisme tubuh. Rumput laut merah Galaxaura rugosa berpotensi sebagai antioksidan. Penelitian ini bertujuan untuk menentukan aktivitas antioksidan ekstrak etanol rumput laut merah Galaxaura rugosa terhadap radikal bebas DPPH berdasarkan nilai IC50. Pada penelitian ini dilakukan pengujian fitokimia dan penentuan aktivitas antioksidan menggunakan metode DPPH (2,2-difenil-1-pikrilhidrazil) yang dilakukan secara kuantitatif menggunakan spektrofotometer UV-Vis. Hasil dari skrining fitokimia ekstrak etanol rumput laut merah mengandung senyawa alkaloid, flavonoid, saponin, steroid dan polifenol serta nilai IC50 ekstrak etanol rumput laut merah sebesar 4,59 ppm sedangkan kontrol positif Vitamin C sebesar 6,64 ppm. Ekstrak etanol rumput laut merah Galaxaura rugosa di Kabupaten Aceh Selatan memiliki potensi antioksidan yang tinggi. dibuktikan dengan nilai IC50 yang yang kecil yaitu < 50 µg/mL.Kata kunci: Antioksidan, Galauxara rugosa, Fitokimia, DPPH

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