Potential of Frangula alnus to contribute to food safety: antibiofilm effect against Staphylococcus aureus

Abstract Contamination by numerous food-borne pathogens is a major challenge facing the food industry daily. Even though there are many strategies in the fight against contamination, pathogens able to attach to different surfaces and form biofilms are the biggest concern. Staphylococcus aureus is a common food-borne pathogen capable of forming biofilms on foods and food contact surfaces. The prevalence of multidrug resistant S. aureus is high in raw products, high-protein foods and processed products. Bearing in mind S. aureus resistance to numerous antibacterial agents, the aim of this study was to investigate antibiofilm activity of an ethyl-acetate extract of the medicinal plant, Frangula alnus, against S. aureus ATCC 25923 and S. aureus ATCC 43300. It was demonstrated that extract reduced survival of both tested strains by up to 67%. Furthermore, quantification of biofilm biomass showed that extract possesses the extraordinary ability to inhibit biofilm formation of both tested strains (up to 91%). On the other hand, the effect on preformed biofilm was less pronounced and measured only for S. aureus ATCC 43300, wherein about 28% of preformed biofilm was eradicated. The results obtained in this study encourage further investigation of F. alnus as a novel antibiofilm agent or preservative in the food industry.

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Biofilm Formation by Staphylococcus aureus Isolated from Food Contact Surfaces in the Dairy Industry of Jalisco, Mexico

Journal of Food Quality ◽

10.1155/2018/1746139 ◽

2018 ◽

Vol 2018 ◽

pp. 1-8 ◽

Cited By ~ 7

Author(s):

María-Guadalupe Avila-Novoa ◽

Maricarmen Iñíguez-Moreno ◽

Oscar-Alberto Solís-Velázquez ◽

Jean-Pierre González-Gómez ◽

Pedro-Javier Guerrero-Medina ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Stainless Steel ◽

Congo Red ◽

Biofilm Formation ◽

Dairy Industry ◽

Food Contact ◽

Biofilm Production ◽

Food Contact Surfaces ◽

Food Borne ◽

Contact Surfaces

Staphylococcus aureus is an important food-borne pathogen able to form biofilms. This pathogen is responsible for outbreaks of food-borne illnesses associated with the consumption of milk and dairy products. The aim of this study was to evaluate the biofilm-forming ability of S. aureus isolates, recovered from food contact surfaces in the dairy industry of Jalisco, Mexico. A total of 84 S. aureus strains were evaluated. The isolates were characterized phenotypically by culture on Congo red agar plates. The ability of the strains to form biofilms was investigated in 96-well flat-bottomed microtiter polystyrene plates. Stainless-steel coupons were used as an experimental surface. Biofilm formation was observed, using epifluorescence microscopy and scanning electron microscopy. Detection of the icaADBC genes in S. aureus was performed by the PCR technique. A total of 52.3% (44/84) of the S. aureus strains contained the icaADBC gene that synthesizes polysaccharide intercellular adhesion (PIA) molecules. On Congo red agar, 75% (63/84) of the S. aureus isolates were biofilm producers, 16.6% (14/84) were non-biofilm formers, and 8.3% (7/84) showed a noncharacteristic phenotype. The biofilm production of the S. aureus strains SA-4E, SA-9, SA-13, and SA-19 on stainless-steel coupons was investigated at 25°C for 8 days, and the detected cell population density was approximately 7.15–7.82 log CFU cm−2. In addition to the ability of biofilm production, it is important to highlight that these strains are potential enterotoxin producers as se genes have been previously detected in their genomes. A part of the ability of biofilm production and the determination of the presence of virulence determinants in the genome of S. aureus can contribute to the pathogenicity of strains. Therefore, vigilant food safety practices need to be implemented in the dairy industries regarding FCS to prevent food-borne infections and intoxications due to S. aureus contamination.

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Antimicrobial and Immunomodulating Activities of Two Endemic Nepeta Species and Their Major Iridoids Isolated from Natural Sources

Pharmaceuticals ◽

10.3390/ph14050414 ◽

2021 ◽

Vol 14 (5) ◽

pp. 414

Author(s):

Neda Aničić ◽

Uroš Gašić ◽

Feng Lu ◽

Ana Ćirić ◽

Marija Ivanov ◽

...

Keyword(s):

Biofilm Formation ◽

Food Industry ◽

Balkan Peninsula ◽

Antimicrobial Activities ◽

Natural Sources ◽

E Coli ◽

Food Borne ◽

Metabolite Profiles ◽

Aspergillus Sp ◽

First Time

Two Balkan Peninsula endemics, Nepeta rtanjensis and N. argolica subsp. argolica, both characterized by specialized metabolite profiles predominated by iridoids and phenolics, are differentiated according to the stereochemistry of major iridoid aglycone nepetalactone (NL). For the first time, the present study provides a comparative analysis of antimicrobial and immunomodulating activities of the two Nepeta species and their major iridoids isolated from natural sources—cis,trans-NL, trans,cis-NL, and 1,5,9-epideoxyloganic acid (1,5,9-eDLA), as well as of phenolic acid rosmarinic acid (RA). Methanol extracts and pure iridoids displayed excellent antimicrobial activity against eight strains of bacteria and seven strains of fungi. They were especially potent against food-borne pathogens such as L. monocytogenes, E. coli, S. aureus, Penicillium sp., and Aspergillus sp. Targeted iridoids were efficient agents in preventing biofilm formation of resistant P. aeruginosa strain, and they displayed additive antimicrobial interaction. Iridoids are, to a great extent, responsible for the prominent antimicrobial activities of the two Nepeta species, although are probably minor contributors to the moderate immunomodulatory effects. The analyzed iridoids and RA, individually or in mixtures, have the potential to be used in the pharmaceutical industry as potent antimicrobials, and in the food industry to increase the shelf life and safety of food products.

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Synthesis and evaluation of new quinazolin-4(3H)-one derivatives as potent antibacterial agents against multidrug resistant Staphylococcus aureus and Mycobacterium tuberculosis

European Journal of Medicinal Chemistry ◽

10.1016/j.ejmech.2019.04.067 ◽

2019 ◽

Vol 175 ◽

pp. 287-308 ◽

Cited By ~ 9

Author(s):

Srikanth Gatadi ◽

Jitendra Gour ◽

Manjulika Shukla ◽

Grace Kaul ◽

Arunava Dasgupta ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Mycobacterium Tuberculosis ◽

Antibacterial Agents ◽

Multidrug Resistant

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Multidrug-resistant Staphylococcus aureus (MDRSA) properties and their adherence ability on stainless steel surfaces at different temperature and time

Food Research ◽

10.26656/fr.2017.4(5).s07 ◽

2020 ◽

Vol 4 (S2) ◽

pp. 57-66

Author(s):

Nur-Syifa' J. ◽

Nor-Khaizura M.A.R. ◽

N.A. Mahyudin ◽

Shafiqa-Atikah M.K. ◽

Ummul-Izzatul Y.

Keyword(s):

Staphylococcus Aureus ◽

Stainless Steel ◽

High Capacity ◽

Food Service ◽

Multidrug Resistant ◽

Diffusion Method ◽

Optimum Growth Temperature ◽

Food Contact Surfaces ◽

Steel Disc ◽

Adherence Ability

Staphylococcus aureus is a pathogenic bacterium that capable to adhere on the processing surfaces that could cause a cross-contamination of foods. In Malaysia, S. aureus has been reported from foods and food-handlers hand at food service environment but the multidrug -resistant S. aureus (MDRSA) and their adherence on stainless steel were limited. This study was intended 1) to isolate S. aureus from food contact surfaces and characterize the isolates for MDRSA properties, and 2) to determine the adherence ability of the MDRSA strains. A total of thirty-eight S. aureus isolated from food premises in Sri Serdang were tested for the antibiotic resistance and it was carried out using five classes of antibiotics; Penicillin (I), Cephalosporins (II), Amino-glycosides (III), Quinolones Fluoroquinolone (IV), and Sulphonamide (V) by the standard procedures of Kirby-Bauer disc diffusion method. The adherence assay was performed on stainless steel disc at 25oC and 37oC on 24, 48 and 72 hrs incubation. As a result, twenty-three S. aureus were found as multidrugresistant towards the antibiotics. All the MDRSA can adhere on stainless steel with a minimum 4.00 log CFU/mL. The adherence of MDRSA on stainless steel during 72 hrs were ranging from 4.11 to 6.55 log CFU/mL and 4.25 to 6.86 log CFU/mL at 25oC and 37oC, respectively. The highest adherence was found on 48 hrs at both temperatures. The MDRSA strains revealed high capacity to adhere on stainless steel at 37oC. As a conclusion, the MDRSA strains shows the strong adherence ability at their optimum growth temperature.

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Bacteriophage-Derived PeptidaseCHAPKEliminates and Prevents Staphylococcal Biofilms

International Journal of Microbiology ◽

10.1155/2013/625341 ◽

2013 ◽

Vol 2013 ◽

pp. 1-8 ◽

Cited By ~ 43

Author(s):

Mark Fenton ◽

Ruth Keary ◽

Olivia McAuliffe ◽

R. Paul Ross ◽

Jim O'Mahony ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Biofilm Formation ◽

Antibacterial Agents ◽

Antimicrobial Agents ◽

Bovine Mastitis ◽

Economic Losses ◽

Food Sector ◽

Staphylococcal Infections ◽

Prevention And Treatment

New antibacterial agents are urgently needed for the elimination of biofilm-forming bacteria that are highly resistant to traditional antimicrobial agents. Proliferation of such bacteria can lead to significant economic losses in the agri-food sector. This study demonstrates the potential of the bacteriophage-derived peptidase,CHAPK, as a biocidal agent for the rapid disruption of biofilm-forming staphylococci, commonly associated with bovine mastitis. PurifiedCHAPKapplied to biofilms ofStaphylococcus aureusDPC5246 completely eliminated the staphylococcal biofilms within 4 h. In addition,CHAPKwas able to prevent biofilm formation by this strain. TheCHAPKlysin also reducedS. aureusin a skin decolonization model. Our data demonstrates the potential ofCHAPKas a biocidal agent for prevention and treatment of biofilm-associated staphylococcal infections or as a decontaminating agent in the food and healthcare sectors.

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Antimicrobial and Antibiofilm Peptides

Biomolecules ◽

10.3390/biom10040652 ◽

2020 ◽

Vol 10 (4) ◽

pp. 652 ◽

Cited By ~ 12

Author(s):

Angela Di Somma ◽

Antonio Moretta ◽

Carolina Canè ◽

Arianna Cirillo ◽

Angela Duilio

Keyword(s):

Antimicrobial Peptides ◽

Biofilm Formation ◽

Bacterial Resistance ◽

Antimicrobial Agents ◽

Multidrug Resistant ◽

Resistant Bacteria ◽

Antibiofilm Activity ◽

Chronic Infections ◽

Planktonic Bacteria ◽

Hostile Environments

The increasing onset of multidrug-resistant bacteria has propelled microbiology research towards antimicrobial peptides as new possible antibiotics from natural sources. Antimicrobial peptides are short peptides endowed with a broad range of activity against both Gram-positive and Gram-negative bacteria and are less prone to trigger resistance. Besides their activity against planktonic bacteria, many antimicrobial peptides also show antibiofilm activity. Biofilms are ubiquitous in nature, having the ability to adhere to virtually any surface, either biotic or abiotic, including medical devices, causing chronic infections that are difficult to eradicate. The biofilm matrix protects bacteria from hostile environments, thus contributing to the bacterial resistance to antimicrobial agents. Biofilms are very difficult to treat, with options restricted to the use of large doses of antibiotics or the removal of the infected device. Antimicrobial peptides could represent good candidates to develop new antibiofilm drugs as they can act at different stages of biofilm formation, on disparate molecular targets and with various mechanisms of action. These include inhibition of biofilm formation and adhesion, downregulation of quorum sensing factors, and disruption of the pre-formed biofilm. This review focuses on the proprieties of antimicrobial and antibiofilm peptides, with a particular emphasis on their mechanism of action, reporting several examples of peptides that over time have been shown to have activity against biofilm.

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Antimicrobial and Antibiofilm Activity against Staphylococcus aureus of Opuntia ficus-indica (L.) Mill. Cladode Polyphenolic Extracts

Antioxidants ◽

10.3390/antiox8050117 ◽

2019 ◽

Vol 8 (5) ◽

pp. 117 ◽

Cited By ~ 17

Author(s):

Federica Blando ◽

Rossella Russo ◽

Carmine Negro ◽

Luigi De Bellis ◽

Stefania Frassinetti

Keyword(s):

Staphylococcus Aureus ◽

Antioxidant Activity ◽

Antioxidant Capacity ◽

Biofilm Formation ◽

Ex Vivo ◽

Trolox Equivalent Antioxidant Capacity ◽

Total Phenolic ◽

Antibiofilm Activity ◽

Opuntia Ficus Indica

Plant extracts are a rich source of natural compounds with antimicrobial properties, which are able to prevent, at some extent, the growth of foodborne pathogens. The aim of this study was to investigate the potential of polyphenolic extracts from cladodes of Opuntia ficus-indica (L.) Mill. to inhibit the growth of some enterobacteria and the biofilm formation by Staphylococcus aureus. Opuntia ficus-indica cladodes at two stages of development were analysed for total phenolic content and antioxidant activity by Oxygen Radical Absorbance Capacity (ORAC) and Trolox equivalent antioxidant capacity (TEAC) (in vitro assays) and by cellular antioxidant activity in red blood cells (CAA-RBC) (ex vivo assay). The Liquid Chromatography Time-of-Flight Mass Spectrometry (LC/MS–TOF) analysis of the polyphenolic extracts revealed high levels of piscidic acid, eucomic acid, isorhamnetin derivatives and rutin, particularly in the immature cladode extracts. Opuntia cladodes extracts showed a remarkable antioxidant activity (in vitro and ex vivo), a selective inhibition of the growth of Gram-positive bacteria, and an inhibition of Staphylococcus aureus biofilm formation. Our results suggest and confirm that Opuntia ficus-indica cladode extracts could be employed as functional food, due to the high polyphenolic content and antioxidant capacity, and used as natural additive for food process control and food safety.

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Promising antibacterial agents against multidrug resistant Staphylococcus aureus

Bioorganic Chemistry ◽

10.1016/j.bioorg.2019.103252 ◽

2019 ◽

Vol 92 ◽

pp. 103252 ◽

Cited By ~ 7

Author(s):

Srikanth Gatadi ◽

Y.V. Madhavi ◽

Sidharth Chopra ◽

Srinivas Nanduri

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Agents ◽

Multidrug Resistant

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The Antimicrobial Effect of Radiant Catalytic Ionization on the Bacterial Attachment and Biofilm Formation by Selected Foodborne Pathogens under Refrigeration Conditions

Applied Sciences ◽

10.3390/app10041364 ◽

2020 ◽

Vol 10 (4) ◽

pp. 1364

Author(s):

Krzysztof Skowron ◽

Karolina Jadwiga Skowron ◽

Justyna Bauza-Kaszewska ◽

Ewa Wałecka-Zacharska ◽

Joanna Kwiecińska-Piróg ◽

...

Keyword(s):

Foodborne Pathogens ◽

Glass Surface ◽

Biofilm Formation ◽

Food Industry ◽

Salmonella Enteritidis ◽

Antimicrobial Effect ◽

Bacterial Attachment ◽

Efficiency Coefficient ◽

Food Contact Surfaces ◽

Reduction Efficiency

The decontamination of food contact surfaces is a major problem for the food industry. The radiant catalytic ionization (RCI) method, based on the ionization process, may be an alternative for conventional decontamination procedures. The advantage of this technique is the possibility of its application to household refrigerating appliances and industrial cold rooms. This study aimed to assess the effect of RCI on the reduction of Campylobacter jejuni, Listeria monocytogenes, and Salmonella Enteritidis from the biofilms formed on a glass surface under refrigeration conditions. Bacterial biofilms were exposed to RCI for 24 h and after 12 (variant I) and 72 h (variant II) of the glass surface contamination. In the last variant (III), the contaminated meat was placed on the glass surface in the refrigerator and subjected to RCI treatment for 72 h. The significantly highest values of absolute reduction efficiency coefficient E were found for the bacterial attachment stage of biofilm formation (variant I). The research proves the efficiency of the RCI method in the reduction of bacteria number from a glass surface.

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