scholarly journals UJI EFEK ANALGESIK EKSTRAK KULIT MANGGIS (GARCINIA MANGOSTANA L.) PADA MENCIT SWISS (MUSS MUSCULUS)

2013 ◽  
Vol 1 (2) ◽  
Author(s):  
Ripka Margaretha Ponggele
Keyword(s):  
Control Group ◽  
Test Substance ◽  
Reference Solution ◽  
Garcinia Mangostana ◽  
Hot Plate ◽  
Plate Method ◽  
Analgesic Effects ◽  
Mangosteen Peel ◽  
Pain Stimuli ◽  
Peel Extract

Background:Indonesia is the country with the second largest plants after Brazil. One of the plants that grow in Indonesia is the mangosteen plant is utilized as a medicine by the people of Indonesia but also by people in some other countries. Mangosteen skin is most often used to treat diseases such as fever, diarrhea, hypertension, antioksidant, antibiotics, anti-inflammatory, and many other diseases. This study aims to prove the presence or absence laboratorik analgesic effects of mangosteen peel extract in mice.Methods:This study uses a sample of nine experimental Swiss mice. The sample was divided into 3 groups, namely the positive control group given the drug tramadol, negative control group given distilled water control and experimental groups were given mangosteen peel extract, each group consisted of three mice. Testing is done by providing analgesic effects of pain stimuli using thermal stimuli (hot plate method) with a temperature of 550C. Given pain stimuli will cause the mice were protecting themselves by jumping response or lick the feet and tail. Testing efekan algesik done before giving the test substance and the reference solution, then at minute 30,60,90 and 120 minutes after administration of the test substance and the reference solution. Tests carried out for 1 minute.Results:mangosteen peel extract has analgesic effects are starting to look at minute 30 to minute 120 with the maximum effect seen at minute 90.Conclusion:mangosteen peel extract (Garcinia mangostana, L) has analgesic effect in swiss mice (Mus musculus)Keywords: Test the effects of analgesics, the mangosteen peel extract, mangosteen skinAbstrakIndonesia merupakan negara yang mempunyai tumbuh-tumbuhan terbesar kedua setelah Brasil. Salah satu tumbuhan yang tumbuh di Indonesia adalah tanaman manggis yang sudah dimanfaatkan sebagai obat oleh penduduk Indonesia bahkan oleh penduduk di beberapa negara lain. Kulit manggis yang paling sering digunakan untuk mengobati penyakit seperti demam, diare, hipertensi, antioksidant, antibiotik, antiinflamasi, dan masih banyak penyakit lainnya. Penelitian ini bertujuan untuk membuktikan secara laboratorik ada tidaknya efek analgesik dari ekstrak kulit manggis pada mencit.Metode : Penelitian ini menggunakan metode eksperimental dengan sampel 9 ekor mencit Swiss. Sampel di bagi dalam 3 kelompok, yaitu kelompok kontrol positif yang diberikan obat tramadol, kelompok kontrol negatif diberikan aquades dan kelompok kontrol eksperimen yang diberikan ekstrak kulit manggis, yang masing-masing kelompok terdiri dari 3 ekor mencit. Pengujian efek analgesik dilakukan dengan memberikan rangsang nyeri menggunakan metode rangsang panas (hot plate method) dengan suhu 550C. Rangsang nyeri yang diberikan akan menyebabakan mencit melakukan perlindungan diri dengan respon melompat atau menjilat kaki dan ekor. Pengujian efek analgesik dilakukan sebelum pemberian zat uji dan larutan pembanding, kemudian pada menit ke 30,60,90 dan menit ke 120 setelah pemberian zat uji dan larutan pembanding. Pengujian dilakukan selama 1 menit.Hasil : Ekstrak kulit manggis mempunyai efek analgesik yang mulai terlihat pada menit ke 30 sampai menit ke 120 dengan efek maksimalnya terlihat pada menit ke 90.Kesimpulan : Ekstrak kulit manggis (Garcinia mangostana, L) memiliki efek analgesik pada mencit swiss (Mus musculus).Kata Kunci : Uji efek analgesik, ekstrak kulit manggis, kulit manggis.

scholarly journals The inhibitory efficacy of flavonoid of mangosteen peel extract (Garcinia Mangostana Linn.) against Lactobacillus Acidophilus biofilm bacteria

2020 ◽  
Vol 10 (2) ◽  
pp. 75
Author(s):  
Dyah Utari Wahyu Ningrum ◽  
Karlina Samadi ◽  
Widya Saraswati
Keyword(s):  
Treatment Group ◽  
Dental Caries ◽  
Lactobacillus Acidophilus ◽  
Bacterial Attachment ◽  
Bacterial Biofilm ◽  
Control Group ◽  
Garcinia Mangostana ◽  
Mangosteen Peel ◽  
Biofilm Bacteria ◽  
Peel Extract

Background: Lactobacillus takes role in the formation of dental plaque which is the main factor that cause dental caries. The bacteria take role in the metabolism of glucose in the mouth, producing organic acids which lower the pH in the oral cavity. This situation can lead to the formation of dental caries because these bacteria can form biofilm as a defense of microorganisms to antibiotics and the immune response. Mangosteen peel has active ingredients such as flavonoid that can be used to inhibit biofilm. The ability of flavonoid compounds in the phenol group can make the bacterial enzyme becomes inactive, causing the activity of glucosyltransferase enzyme that usually used by bacteria to synthesize sucrose in the medium becomes glucan. As a result, bacterial biofilm formation is inhibited because the amount of glucan as a medium of bacterial attachment is limited, but until now the effectivity dose of flavonoid from mangosteen peel extract (Garcinia mangostana Linn.) is has not known yet Purpose: The aim of this study was to determine the inhibitory efficacy of flavonoid of mangosteen peel extract against the formation of Lactobacillus acidophilus bacterial biofilm. Methods: Bacteria that had been formed into biofilms was studied in two times treatment, one concentration reviewed by flavonoid from mangosteen peel extract and without reviewed by flavonoid from mangosteen peel extract. The treatment group was incubated at 37°C until day 8 since the first day incubated. The treatment group was washed with phosphate buffered saline (PBS) 4 times and dried. The treatment group stained with 0.2 ml 0.1% crystal violet and 15 min incubation. The treatment group was rinsed with distilled water 3 times and then dried. Solvent DMSO 100% was added as much as 0.1 ml in each well. Microtiter plate was shaken for 1 minute and then placed in to the microplate reader and then the OD (Optical Density) can be read. Results: There were significant differences between the inhibitory efficacy of flavonoid of mangosteen peel extract against Lactobacillus acidophilus biofilm bacteria, the control group and group treatments (p <0.05). Conclusion: Flavonoid of mangosteen peel extract with the concentration of 0.78% had inhibitory effect against Lactobacillus acidophilus biofilm bacteria

scholarly journals EVALUATION OF ANALGESIC ACTIVITY OF MURRAYA KOENIGII AND CORIANDRUM SATIVUM LEAVES EXTRACT IN ANIMAL MODEL.

2018 ◽  
Vol 11 (1) ◽  
pp. 328
Author(s):  
Kartik Salwe J ◽  
Mirunalini R ◽  
Jervin Mano ◽  
Manimekalai K
Keyword(s):  
Analgesic Activity ◽  
Analgesic Effect ◽  
Coriandrum Sativum ◽  
Control Group ◽  
Tail Flick ◽  
Murraya Koenigii ◽  
Hot Plate ◽  
Plate Method ◽  
Standard Drug ◽  
Soxhlet Apparatus

 Objective: The objective of the study was to investigate the analgesic activity of hydroalcoholic extract of Murraya koenigii and Coriandrum sativum leaves and compared it with standard drug in an animal model.Methods: Hydroalcoholic extracts of M. koenigii and C. sativum leaves were obtained using Soxhlet apparatus. The central analgesic property was screened by hot plate method in mice and tail flick method in rats. The pain reaction time (PRT) was measured at 30, 60, and 120 min. The peripheral analgesic activity was evaluated by acetic acid induced writhing in mice.Results: In hot plate method M. koenigii leaves extract at both doses and tramadol showed significant increase in PRT at 30, 60, and 120 min compared with control group. C. sativum leaves extract showed significant increase in PRT only at 60 and 120 min compared to control group. In tail flick method M. koenigii leaves extract at both doses, higher dose of C. sativum leaves extract and tramadol showed significant increase in PRT at 30, 60, and 120 min compared with control group. Higher dose of M. koenigii leaves extract (200 mg/kg) was comparable with standard drug tramadol in both the methods. M. koenigii leaves extract at both dose showed significant reduction in the number of writhing but C. sativum leaves extract failed to show any significant reduction in the number of writhing compared with control. Higher dose of M. koenigii leaves extract was comparable with standard drug tramadol.Conclusion: M. koenigii leaves extract showed both peripheral and central analgesic effect while C. sativum leaves extract showed only peripheral analgesic effect.

scholarly journals FORMULASI NANOEMULGEL EKSTRAK KULIT MANGGIS (Garcinia Mangostana L.)

2019 ◽  
Vol 1 (3) ◽  
pp. 166-176
Author(s):  
Hilda Damayanti ◽  
Saleh Wikarsa ◽  
Garnadi Jafar
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Particle Size Analysis ◽  
Size Analysis ◽  
Garcinia Mangostana ◽  
Mangosteen Peel ◽  
Dpph Method ◽  
Nanoemulsion Gel ◽  
Materials Used ◽  
Peel Extract

Antioxidant-containing cosmetic has antiaging therapy that can inhibit the free radical formation. Mangosteen peel extract has very strong antioxidant activity. To enhance the effect and comfortness of mangosteen peel extract use on the skin, it could be made into nanoemulgel. The article provides the information about method of preparation and evaluation of nanoemulsion-gel. The purpose of this study was to formulate a stable microemulgel of mangosteen peel extractusing halal materials declared halal according to Islamic Shari’a.  the materials used don’t contain carrion, blood, pig and/ animals that don’t conform to Islamic Shari’a. Microemulgel mangosteen peel extract was made by varying plantacare® 1200 UP concentration as cosurfactant (5, 10, 15, 20 and 25%) in the microemulsion and it was incorporated into the gel base. Evaluations were included the antioxidant activity test and organoleptic, pH, viscosity, stability, particle size analysis and panelist test. The antioxidant activity determined by DPPH method showed that IC50 value of mangosteen peel extract was 5.54 ppm. The third microemulsion formula containing cosurfactant of 15% resulted in the best results in that the parameter of the product can be penetrated by ray laser was at particle size of  23.65 nm, was determined by tranmission Electron Microscopy (TEM). Microemulgel containing Viscolam® MAC 10 of seven percent gave the stable formula proofed by freeze thaw and sentrifuga test. The five microemulgel formulations were stable.    

scholarly journals Analgesic Effect of Zolmitriptan Nanoparticles in Experimental Animal Models

2018 ◽  
Vol 3 (07) ◽  
Author(s):  
Mr. Farshid Ali Naghi Zadeh Khezri ◽  
Dr. Shachindra L. Nargund ◽  
Dr. C. S. R. Lakshmi ◽  
Dr. L. V. G. Nargund ◽  
Dr. Reza Alisani ◽  
...  
Keyword(s):  
Reaction Time ◽  
Nasal Spray ◽  
Drug Administration ◽  
Chitosan Nanoparticles ◽  
Reaction Times ◽  
Control Group ◽  
Test Drug ◽  
Hot Plate ◽  
Plate Method ◽  
Standard Drug

Background: Zolmitriptan is used to treat migraine and certain other headaches. This study has been done to prepare specific Zolmitriptan Nano-nasal spray (Z-NPS) by ion gelation technique to evaluate the efficacy of analgesic activity of test drug as a nano nasal spray of Zolmitriptan (Z-NPS) and conventional preparation as a standard drug (Zolmist) against Nitro-glycerine induced migraine. The study was performed on mice by using hot plate method. Methods: In this study male Swiss albino mice weighing between 25-30 grams were taken and divided into 4 groups with 6 mice in each group. Zolmitriptan loaded chitosan nanoparticles (Z-NPS) was administered nasally (using specific inhalation mask) at a dose of 5 mg/kg and was compared with the control group which received water for injection and the standard drug Zolmitriptan (Zolmist) at a same dose in mice induced migraine using hot plate method. Reaction times were measured at 10, 20, 30 and 60 minutes after drug administration. Results: The test drug (Z-NPS) at a dose of 5 mg/kg after 10 minutes of drug administration showed an increase in the reaction time compared to the standard drug (Zolmist) at same dose of drug administration. However, Z-NPS showed significant increase in the reaction time after 10 minutes as compared to Zolmist. Conclusion: Zolmitriptan has significant analgesic properties in central-analgesic model i.e. Hot-plate method. The test drug could be used for anti-migraine activity.

scholarly journals Decrease of LDL Cholesterol through Increase of HDL Cholesterol by Administering Garcinia mangostana L. Peel Extract in White Mice

2021 ◽  
Vol 57 (4) ◽  
pp. 298
Author(s):  
Hasyim As'ari
Keyword(s):  
Ldl Cholesterol ◽  
Hdl Cholesterol ◽  
Negative Control ◽  
Laboratory Research ◽  
Control Group ◽  
Garcinia Mangostana ◽  
Control Group Design ◽  
Significance Level ◽  
High Fiber ◽  
Peel Extract

Highlight:Ethanol extract of Garcinia mangostana L. peel reduce malondialdehyde.Garcinia mangostana L. peel extract can increase non-radical products which impact in decreasing LDL cholesterol and increasing HDL cholesterol. Abstract:Atherosclerosis contributes to coronary heart disease which may lead to fatality. High cholesterol consumption, stress, and smoking can increase LDL cholesterol in the blood. Consumption of unsaturated fats, high fiber foods, exercise, quitting smoking, losing weight, and giving hypolipidemic drugs, especially herbs, can increase HDL cholesterol and decrease LDL cholesterol. Garcinia mangostana L. peel  extract can decrease LDL cholesterol by increasing reverse HDL cholesterol transport to the liver. The study used post test control group design. This study was experimental laboratory research with population of hypercholesterolemic male white mice aged 3-4 weeks with 100-200 grams weight. The HDL and LDL cholesterol data were collected through an enzymatic method by spectrophotometer. This study used analysis of variance (Anova) with significance level of α <0.05. The experiment divided the subjects into positive and negative control groups with dosage variations of 50, 150, 250, and 350 mg/kgBW.  Examination of hypercholesterolemia in white mice was conducted on the 8th day. The examination of HDL and LDL cholesterol given peel extract of Garcinia mangostana L. was conducted on the 22nd day. The analysis showed that giving Garcinia mangostana L. peel extract for various dosages could significantly decrease LDL cholesterol and increase HDL cholesterol (p <0.05). Peel extract of Garcinia mangostana L. that contained mangosteen could increase non-radical products that could prevent the transfer of ester cholesterol from HDL to VLDL which impact in increasing HDL cholesterol and decreasing LDL cholesterol.

scholarly journals Effect of mangosteen (Garcinia mangostana L.) peel extract as an antibiotic growth promoter on growth performance and antibiotic resistance in broilers

2020 ◽  
Vol 13 (4) ◽  
pp. 796-800
Author(s):  
Okti Herawati ◽  
Tri Untari ◽  
Marla Anggita ◽  
Sidna Artanto
Keyword(s):  
Body Weight ◽  
Antibiotic Resistance ◽  
Weight Gain ◽  
Feed Intake ◽  
Body Weight Gain ◽  
Post Treatment ◽  
The Body ◽  
Garcinia Mangostana ◽  
Mangosteen Peel ◽  
Peel Extract

Background and Aim: Antibiotic resistance poses a risk to human health and has therefore been the focus of research. One of the causes of this resistance is the use of antibiotics as feed additives for animal nutrition. The development of antibiotic resistance in poultry through nutrition feed has drawn attention to the need for alternative antibiotic growth promoters (AGPs). Mangosteen (Garcinia mangostana L.), as a natural source of bioactive phytochemicals, is a potential AGP, but the effect of mangosteen-based treatment on antibiotic resistance in poultry has not been reported to date. Therefore, the aim of this study was to evaluate the effects of mangosteen peel extract as an AGP on body weight gain, feed conversion rate (FCR), and the antibiotic resistance in broilers. Materials and Methods: In this study, 30 1-day-old broiler chicks were divided into three groups. Group A (control) was not administered any treatment in the feed, Group B was treated with 0.3 g/kg colistin as the AGP in the feed, and Group C was treated with 2% mangosteen peel extract as the AGP in the feed; the treatments were administered for 30 days. The observed parameters included the effect of the treatments on body weight gain, feed intake, FCR, and the presentation of antibiotic resistance before and after the treatments (pre-treatment and post-treatment, respectively). Results: Post-treatment, the body weight gain, and feed intake in the broilers were not significantly different among all the groups; however, the body weight gain and FCR were significantly different between the control group and the treatment groups in the 3rd week of treatment and were not significantly different between Groups B and C. The rate of antibiotic resistance to chloramphenicol increased significantly by 40% in Group B post-treatment, but no such increase was observed in Groups A and C. Conclusion: The findings of our study indicate that compared with using colistin as an AGP using mangosteen peel extract as a natural AGP did not have any significantly different effect on body weight gain, feed intake, and FCR (p>0.05) but had a significantly different effect on the rate of antibiotic resistance in broilers (p<0.05). This study indicates the usefulness of mangosteen for improving the overall growth and production performance of broilers without increasing their antibiotic resistance.

scholarly journals Microbiological characteristics of goat’s milk kefir with the addition of mangosteen (Garcinia mangostana L.) peel extract

2020 ◽  
pp. 65-73
Author(s):  
Listya Purnamasari ◽  
Defi S. Cahyaningrum ◽  
Mochammad S. Sunarno ◽  
Viagian Pastawan ◽  
Epi Tauik
Keyword(s):  
Fermented Milk ◽  
Plate Count ◽  
Garcinia Mangostana ◽  
Milk Products ◽  
Mangosteen Peel ◽  
Goat’S Milk ◽  
Microbiological Characteristics ◽  
Goat's Milk ◽  
Peel Extract ◽  
Fermented Milk Products

Kefir is one of the fermented milk products. The addition of mangosteen peel extract as a source of antioxidant was aimed to increase the functionality of fermented milk products. The purpose of this research was to study the microbiological characteristics of goat’s milk kefir addition with different concentration (0%, 1%, 2%, and 3%) of mangosteen peel extract. The extract of mangosteen peel was purchased from PT. Haldin Pacific Semesta. The results showed that the addition of mangosteen peel extract did not affect significantly on total lactic bacteria, total plate count, and total coliform, but it affected significantly on total mold and yeast. Total lactic acid bacteria in kefir decreased from kefir grain and bulk starter of kefir, but those populations still same with the standard of microorganisms in prebiotic which is 107 CFU/ml. The conclusion of this study, mangosteen peel extract can be used to decrease total microbial such as mold/yeast at concentration 3% dan 4%.

scholarly journals Effect of mangosteen peel extract on BPA-exposed murine during gestation

2022 ◽  
Vol 43 (1) ◽  
pp. 20-25
Author(s):  
K.L. Loh ◽  
◽  
P.J. Kwong ◽  
M.Y. Chan ◽  
G.C. Tan ◽  
...  
Keyword(s):  
Litter Size ◽  
Treated Group ◽  
Daily Weight Gain ◽  
Control Group ◽  
Protective Effects ◽  
Maternal Weight ◽  
Female Ratio ◽  
Mangosteen Peel ◽  
Pregnant Mice ◽  
Peel Extract

Aim: To evaluate the potential protective effects of mangosteen peel extract against BPA-induced abnormalities on-pregnant mice fetus at implantation stage and offspring at post-parturition. Methodology: Pregnant mice were orally administered with BPA (100mg kg-1 b.wt.) and mangosteen peel extract (200mg kg-1 b.wt.) for 16 days. In order to evaluate the effect of MPE treatment on fetus at implantation stage, the pregnant mice were euthanized at day 18 and the fetus number and morphology were examined. Another group of treated dams, were allowed to undergo parturition for evaluating the of maternal weight, litter size and offspring sex-skewness. Results: Upon feeding Mangosteen peel extract (MPE), the average daily weight gain of dams were not significantly different from the control and BPA treated dams. The fetus derived from BPA treated dams were detected with abnormalities such as under development, haemorrhage and absence of vein, whereas fetus from dam treated with MPE and BPA as well as control were normal. The average litter size of all the treatment groups were not significantly different from the control group. BPA treated mice had lower pups survival up to 6 weeks compared to the groups treated with MPE and control. Test of proportion analysis showed BPA-treated group had significantly higher fraction female ratio. Interpretation: BPA is known as endocrine disruptor causing oxidative stress to female reproductive system, hence mangosteen peel extract contains antioxidant substances that have the potential to ameliorate the adverse effects of BPA exposure on dams during pregnancy and its fetus development.

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