scholarly journals Heritability of quantitative characters of local black rice (Oryza sativa L.) results of generation F3

2021 ◽  
Vol 886 (1) ◽  
pp. 012128
Author(s):  
Sutriana S ◽  
Muh Riadi ◽  
Feranita Haring
Keyword(s):  
Oryza Sativa L ◽  
Black Rice ◽  
Productivity Level ◽  
Full Black ◽  
Agronomic Character ◽  
Randomized Design ◽  
Effect Of Treatment ◽  
Heritability Analysis ◽  
Crop Variety ◽  
Efficient Selection

Abstract Local Black rice is a food crop variety that has a low productivity level and a longer planting time than other colored rice. In addition, black rice contains anthocyanins, which are antioxidant molecules that can counteract free radicals in the human body. Anthocyanin levels can be increased by producing full black potential lines. Effective and efficient selection with heritability analysis. This study aims to determine the heritability of the agronomic character of black rice from the F3 generation line. The study was conducted in the Manipi Sinjai rice field in February – August 2020. The experiment used was a completely randomized design, there were 17 lines with 3 replications. The data obtained were analyzed by means of variance, and if there was a significant effect of treatment, further tests were carried out with the BNT 0.05 test. In addition, the heritability of the observed characters was determined. The results showed that the character of plant height in the flowering phase, days to 50% flowering, and the percentage of grain with full black endosperm had heritability values with high criteria.

scholarly journals PENGARUH PUPUK ORGANIK CAIR UNITAS SUPER ASAL Chromolaena Odorata TERHADAP PERTUMBUHAN DAN HASIL PADI HITAM (Oryza sativa L.)

2018 ◽  
Vol 8 (2) ◽  
pp. 15 ◽  
Author(s):  
Jamilah Munir ◽  
Beben Kurniawan ◽  
Zahanis Zahanis
Keyword(s):  
Oryza Sativa ◽  
Harvest Index ◽  
Oryza Sativa L ◽  
Growth And Yield ◽  
Panicle Length ◽  
Chromolaena Odorata ◽  
Black Rice ◽  
Urban Village ◽  
Randomized Design ◽  
Completely Randomized Design

The aim of this research was to know the influence of concentration and interval of Liquid organic manure (LOM) Unitas Super  (US) form Chromolaena odorata on growth and yield of black rice (Oryza sativa L.). The experiment was conducted from October 2016 to March 2017 in Lubuk Minturun Urban Village, Koto Tangah Sub-district, Padang. The experiments were arranged in Completely Randomized Design, with 6 doses of POC Unitas Super (US) (v/v) with water, ie; 0 ml L-1 (F1); 50 ml L-1, 1 time a week (F2), 50 ml L-1, 1 time 2 weeks (F3), 100 ml L-1, a weekly (F4), 100 ml L-1, 1 time every 2 weeks (F5); 150 ml L-1 POC US, 1 time every 3 weeks (F6), and 3 replications. The data obtained were analyzed using analysis of variance (ANOVA) 5% real level. If the treatment had a significant effect, it was continued with LSD test of 5% real level. Parameters observed include; Plant height, the maximum number of tillers and productive, flowering age, harvest, panicle length, the number of grains per panicle, weight of 1000 seeds, the weight of dry grain per hectare, percentage of empty grain and harvest index. The experimental results proved that there was an influence of LOM Unitas Super treatments on maximum tillers, empty grain, panicle length and dry grain harvest, and some other parameters were not significance. The conclusion was the giving of 50 ml L-1 POC Unitas Super given every week is the best treatment. The highest rice yield reached 6.79 tons ha-1 dry milled grain, with harvest index reached 0.49.  

scholarly journals Subcellular Localization and Vesicular Structures of Anthocyanin Pigmentation by Fluorescence Imaging of Black Rice (Oryza sativa L.) Stigma Protoplast

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 685
Author(s):  
Enerand Mackon ◽  
Yafei Ma ◽  
Guibeline Charlie Jeazet Dongho Epse Mackon ◽  
Qiufeng Li ◽  
Qiong Zhou ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Subcellular Localization ◽  
Oryza Sativa L ◽  
Scientific Evidence ◽  
Brown Rice ◽  
The Body ◽  
Central Vacuole ◽  
In Planta ◽  
Black Rice

Anthocyanins belong to the group of flavonoid compounds broadly distributed in plant species responsible for attractive colors. In black rice (Oryza sativa L.), they are present in the stems, leaves, stigmas, and caryopsis. However, there is still no scientific evidence supporting the existence of compartmentalization and trafficking of anthocyanin inside the cells. In the current study, we took advantage of autofluorescence with anthocyanin’s unique excitation/emission properties to elucidate the subcellular localization of anthocyanin and report on the in planta characterization of anthocyanin prevacuolar vesicles (APV) and anthocyanic vacuolar inclusion (AVI) structure. Protoplasts were isolated from the stigma of black and brown rice and imaging using a confocal microscope. Our result showed the fluorescence displaying magenta color in purple stigma and no fluorescence in white stigma when excitation was provided by a helium–neon 552 nm and emission long pass 610–670 nm laser. The fluorescence was distributed throughout the cell, mainly in the central vacuole. Fluorescent images revealed two pools of anthocyanin inside the cells. The diffuse pools were largely found inside the vacuole lumen, while the body structures could be observed mostly inside the cytoplasm (APV) and slightly inside the vacuole (AVI) with different shapes, sizes, and color intensity. Based on their sizes, AVI could be grouped into small (Ф < 0.5 um), middle (Ф between 0.5 and 1 um), and large size (Ф > 1 um). Together, these results provided evidence about the sequestration and trafficking of anthocyanin from the cytoplasm to the central vacuole and the existence of different transport mechanisms of anthocyanin. Our results suggest that stigma cells are an excellent system for in vivo studying of anthocyanin in rice and provide a good foundation for understanding anthocyanin metabolism in plants, sequestration, and trafficking in black rice.

scholarly journals Co-Microencapsulated Black Rice Anthocyanins and Lactic Acid Bacteria: Evidence on Powders Profile and In Vitro Digestion

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2579
Author(s):  
Carmen-Alina Bolea ◽  
Mihaela Cotârleț ◽  
Elena Enachi ◽  
Vasilica Barbu ◽  
Nicoleta Stănciuc
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Oryza Sativa L ◽  
Milk Proteins ◽  
Ethanolic Extract ◽  
Dry Weight ◽  
Hot Water ◽  
Raw Material ◽  
Black Rice

Two multi-functional powders, in terms of anthocyanins from black rice (Oryza sativa L.) and lactic acid bacteria (Lactobacillus paracasei, L. casei 431®) were obtained through co-microencapsulation into a biopolymer matrix composed of milk proteins and inulin. Two extracts were obtained using black rice flour as a raw material and hot water and ethanol as solvents. Both powders (called P1 for aqueous extract and P2 for ethanolic extract) proved to be rich sources of valuable bioactives, with microencapsulation efficiency up to 80%, both for anthocyanins and lactic acid bacteria. A higher content of anthocyanins was found in P1, of 102.91 ± 1.83 mg cyanindin-3-O-glucoside (C3G)/g dry weight (DW) when compared with only 27.60 ± 17.36 mg C3G/g DW in P2. The morphological analysis revealed the presence of large, thin, and fragile structures, with different sizes. A different pattern of gastric digestion was observed, with a highly protective effect of the matrix in P1 and a maximum decrease in anthocyanins of approximatively 44% in P2. In intestinal juice, the anthocyanins decreased significantly in P2, reaching a maximum of 97% at the end of digestion; whereas in P1, more than 45% from the initial anthocyanins content remained in the microparticles. Overall, the short-term storage stability test revealed a release of bioactive from P2 and a decrease in P1. The viable cells of lactic acid bacteria after 21 days of storage reached 7 log colony forming units (CFU)/g DW.

scholarly journals Response of black rice (Oryza sativa L Indica.) to organic fertilizer and seedling number on grain yield, amylum, antioxidant, and thiamin hydrochloride contents

2018 ◽  
Vol 192 ◽  
pp. 03032
Author(s):  
Okti Herliana ◽  
A. H. Saeful Anwar ◽  
Ida Widiyawati
Keyword(s):  
Oryza Sativa L ◽  
Organic Fertilizer ◽  
Organic Fertilizers ◽  
Chicken Manure ◽  
Thiamine Hydrochloride ◽  
Anthocyanin Content ◽  
Black Rice ◽  
Central Java ◽  
Combination Treatments ◽  
Main Plot

This study aimed to investigate the influence of organic fertilizers and seedling numbers each holes of black rice on yield, antioxidant, amylum, and thiamin hydroclorine contents, and to observe the interaction effect between types of organic fertilizers and seedling numbers each hole planting. This study was conducted on rice field in Karanglewas Kidul Village, Karanglewas, Banyumas Regency, Central Java from April until September 2016. The location altitude in this study for about 93 meters above the sea level. The study was arranged by Split Plot Design by the main plot consist with three types of organic fertilizers that was chicken, goat, and cow manures, and the sub plot was consists by seedling number were, three, two, and one seedling by each holes, with three replicates of each combination treatments. The result showed that chicken manure treatment provide the best influences on grain weight per hectare by 5.154 tons, amylum content by 33.86%, anthocyanin content by 275.40 ppm, and Thiamin hydrocloride content by 0.056 mg/10g. The three seedling each hole provide the best influences on amylum content by 33.78%, anthocyanin content by 275.18 ppm, and thiamine hydrochloride content by 0.058 mg/10g.

scholarly journals Agrobacterium Tumefaciens Mediated Transformation of rolC::Hd3a-GFP in Black Rice (Oryza Sativa L. cv. Cempo Ireng) to Promote Early Flowering

2015 ◽  
Vol 14 ◽  
pp. 469-473 ◽  
Author(s):  
Yekti Asih Purwestri ◽  
Resta Dewi Komala Sari ◽  
Lisa Novita Anggraeni ◽  
Aries Bagus Sasongko
Keyword(s):  
Oryza Sativa ◽  
Agrobacterium Tumefaciens ◽  
Oryza Sativa L ◽  
Early Flowering ◽  
Black Rice

scholarly journals PENGARUH KOTORAN AYAM DAN PACLOBUTRAZOL TERHADAP JUMLAH ANAKAN DAN TINGGI BATANG PADI HITAM (Oryza sativa L.) KABUPATEN TAPANULI SELATAN

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Romaya Sitha Silitonga ◽  
Jumaria Nasution
Keyword(s):  
Vitamin E ◽  
Rice Plant ◽  
Oryza Sativa L ◽  
Block Design ◽  
Vitamin B1 ◽  
Brown Rice ◽  
Tiller Number ◽  
Chicken Manure ◽  
Black Rice ◽  
Randomized Block Design

ABSTRACT Black rice is very potential to be expanded because the content of anthocyanin, vitamin B1, and vitamin E black rice is higher than brown rice or white rice. At present, black rice producers are in Java, whereas in Sumatra, South Tapanuli District, Angkola Sub-District is a new type of black rice for rice farmers, so it is necessary to introduce and cultivate. The aims of the research was to determine the effect of chicken manure and paclobutrazol concentration on the tiller number and stems height of the rice plant in Batang Angkola District, South Tapanuli. This research method used was Randomized Block Design Factorial with two factors. The first factor was application of chicken manure (C) with dosage 0 g/pot 3 g/pot (C1), 6 g/pot (C2) and 9 g/pot (P3). The second factor was application paklobutrazol (P) with concentrations of 0 ppm (P0), 25 ppm (P1), 50 ppm (P2) and 75 ppm (P3). Statistical analysis used Anova 5%. If there is a difference followed by DMRT. The result showed that application of chicken manure at 9 g/pot (C3) and paclobutrazol 75 ppm (P3) were produce more tiller number and able to decrease height of black rice plant. Key words : Black Rice, Chicken manure, Paklobutrazol, South Tapanuli District, Tiller number, ABSTRAK Padi hitam sangat potensial dikembangkan karena kandungan antosianin, vitamin B1, dan vitamin E padi hitam lebih tinggi dari beras merah maupun beras putih. Adapun daerah penghasil beras hitam saat ini masih terdapat di daerah-daerah Pulau Jawa, sementara di Pulau Sumatera, khususnya di Kabupaten Tapanuli Selatan, Kecamatan Batang Angkola beras hitam masih tergolong tanaman padi jenis baru bagi para petani beras sehingga perlu dilakukan upaya perkenalan dan budidaya di daerah ini. Penelitian ini bertujuan mengetahui pengaruh konsentrasi kotoran ayam dan paclobutrazol terhadap jumlah anakan dan tinggi batang padi hitam yang tumbuh di Kecamatan Batang Angkola, Tapanuli Selatan. Metode penelitian yang digunakan adalah Rancangan Acak Kelompok Faktorial dengan 2 faktor. Faktor pertama, aplikasi Kotoran ayam (C) dengan dosis 0 g/pot (C0), 3 g/pot (C1), 6 g/pot (C2) dan 9 g/pot. Faktor kedua, paklobutrazol (P) dengan konsentrasi 0 ppm (P0), 25 ppm (P1), 50 ppm (P2) dan 75 ppm (P3). Data dianalisis menggunakan statistik analisis variansi pada taraf kesalahan 5%. Apabila ada beda nyata dilanjutkan dengan DMRT. Hasil penelitian aplikasi Kotoran ayam 9 g/pot (C3) dan paclobutrazol 75 ppm (P3) menghasilkan jumlah anakan lebih banyak dan dapat menekan pertumbuhan tinggi batang padi hitam yang ditanam. Kata Kunci : Jumlah anakan, Kabupaten Tapanuli Selatan, Kotoran Ayam, Paclobutrazol, Padi hitam.

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