Mutation induction in the pineapple (Ananas comosus L. Merr) using colchicine

Abstract Pineapple is a tropical fruit that has high economic value. Mutation is a method to increase plant diversity which plays an essential role in plant improvement. This study aimed to induce mutations in pineapple using colchicine. This study was arranged in a factorial completely randomized design. The first factor was pineapple genotypes (i.e., Gemilang, Bangka, Queen, and Suska Kualu) and the second factor was colchicine concentration (i.e., 0.03%, 0.04%, 0.05%, and control). There were 16 treatments with five repetitions so the total was 80 experimental units. Observations were made for three months after treatment in the vegetative phase. Observation parameters included leaf shape, leaf color, plant height, number of leaves, leaf width, and length of stomata. This study indicated that the interaction between genotype and colchicine significantly differed in plant height and the number of leaves. Colchicine significantly increased the length of stomata, and genotype significantly affected all observed parameters. This study concluded that 0.05% colchicine significantly increased plant height (26.67%) and the number of leaves (48.98%) in the Gemilang genotype but decreased plant height and leaf number in other genotypes. This study suggests the need for observation of the flowering phase and fruit quality due to colchicine treatment.

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Growth and Yield of Tomato (Lycopersicon esculentum Mill.) as Influenced by Poultry Manure and Biochar in Two (2) Soil Depths

Journal of Experimental Agriculture International ◽

10.9734/jeai/2020/v42i330484 ◽

2020 ◽

pp. 55-63

Author(s):

F. B. Musa ◽

F. O. Abiodun ◽

A. R. Falana ◽

B. H. Ugege ◽

R. V. Oyewumi ◽

...

Keyword(s):

Plant Height ◽

Poultry Manure ◽

Botanical Garden ◽

Growth And Yield ◽

Tomato Plants ◽

Randomized Design ◽

Significant Difference ◽

Number Of Leaves ◽

Collar Diameter ◽

And Control

Background and Objective: Unproductive soil has been a major problem to farmers, recent interest in the use of a stable organic manure (biochar) as soil amendment and poultry manure to increase soil fertility is also increasing. Hence, the experiment was conducted to assess the condition of poultry manure and biochar on the growth and yield of tomato. Materials and Methods: A greenhouse experiment was conducted at the Federal College of Forestry Ibadan, using polyethylene bag with 5 kg soil collected at two (2) soils depths, 0-15 cm and 15-30 cm depth at the botanical garden, Federal College of Forestry Ibadan. The experiment was arranged in a completely randomized design (CRD) with four replicates. The treatments used were poultry manure (5t/ha), biochar (10t/ha), mixture of poultry manure and biochar (1:1) and control (no amendment).The tomato seeds (UC82B) were sourced from NIHORT, Ibadan and raised in a germination basket. Data were collected weekly on plant height, collar diameter and number of leaves, 50% days to flowering and yield of tomato plants from two weeks after transplanting (WAT). Data collected were analyzed statistically using ANOVA while means were separated using LSD (p<0.05) where applicable. Results: In 0-15 cm soil, the growth variables (plant height, number of leaves and collar diameter) increased with various treatments used. The highest tomato height was observed with the application of biochar (10t/ha). All the treatments applied affected the number of tomato leaves at all weeks after transplanting (WAT) with poultry manure 5t/ha having the highest number of leaves at 7WAT, while collar diameter was significantly affected by various treatments from 2 to 5 WAT, with the mixture of P.M + BIO (1:1) recording the highest value and the control plot recorded the least values in all weeks. However, in 15-30 cm soil, there was significant difference in the number of leaves of tomato among all the amended soil from 5 to 7 WAT, the highest tomato leaves were obtained when the soil was amended with poultry manure (5t/ha) and can be compared with other treatments. Interaction of poultry manure and biochar(1:1) and poultry manure 5 t/ha resulted in highest height of tomato plant from 3-7 WAT as compared to the control that had the least value but were not significantly different from the sole application of biochar 10t/ha. Interaction of poultry manure + biochar (1:1) and poultry manure (5t/ha) gave the highest tomato flowering and fruits. Conclusion: Based on this result, biochar and poultry manure when added to soil, boosted the mineralization of the soil after amendment.

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Phenotipical Characters of Melon (Cucumis melo L.) in Response to Karst Critical Land

BIOTROPIC The Journal of Tropical Biology ◽

10.29080/biotropic.2018.2.1.1-10 ◽

2018 ◽

Vol 2 (1) ◽

pp. 1-10

Author(s):

Yuanita Rachmawati ◽

Budi Setiadi Daryono ◽

Ganies Riza Ariestya

Keyword(s):

Water Content ◽

Plant Height ◽

Research Result ◽

Control Plant ◽

Fruit Weight ◽

Phenotypic Characters ◽

Randomized Design ◽

Number Of Leaves ◽

And Control ◽

Cucumis Melo L

Yogyakarta Agroecosystem has 158,600 ha of critical land spread over three zones. Two areas are Karst Land, located on Agroecosystem II includes Gunungsewu Hills, Gunungkidul and III covers Dlingo Bantul Hills and Sentolo Hills Kulon Progo Regency.. Karst Land is certainly provides stress to plants. These research purposes are examining the phenotype character of superior melon Cultivar TACAPA compare to parents and offsprings phenotypes. The phenotype characters are based on plant height, leaf number, time of melon flowering, water content of plants, and fruit and seed productivity. This experiment was done by Split Plot Design with Completely Randomized Design (CRD) with 4 kinds of treatment (control plant media, Gunungsewu, Dlingo, and Sentolo), 7 experimental units cultivars: TACAPA, TA, TP, PT, AT, Action 434, PI 371795), and 4 replications. Research result reveals that most of the phenotypic characters including plant height, number of leaves, fruit weight, and number of seeds produced have relatively no significant effect between treatment and control, while the phenotypic first time flowering time and water content of the plant, have a noticeable difference.

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GROWTH RESPONSE OF BEHA GRASS (Brachiaria humidicola) FERTILIZED WITH GOAT MANURE

Indonesian Journal Of Animal Agricultural Science (IJAAS) ◽

10.33772/ijaas.v2i3.14080 ◽

2021 ◽

Vol 2 (3) ◽

Author(s):

Budiman Budiman ◽

Natsir Sandiah ◽

La Malesi

Keyword(s):

Plant Height ◽

Root Length ◽

New Production ◽

Brachiaria Humidicola ◽

Randomized Design ◽

Length Data ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Goat Manure ◽

Difference Test

This study aimed to determine the best dosage of goat manure as a natural fertilizer on Beha grass(Brachiaria humidicola). This study used 64 poles of Beha grass grown in polybags divided into 16plots, and it used a completely randomized design (CRD) to analyze the data. This study consist of 4treatments that is P0 (0 ton/ha of goat manure), P1 (10 ton/ha of goat manure), P2 (15 ton/ha of goatmanure), and P3 (20 ton/ha of goat manure). This study's variables were plant height, number of leaves,numbers of tillers, new production, and root length. Data analysis used ANOVA variance continued withreal honest difference test. The result of this study showed that averages of plant height (cm) wereP0=26, P1=50.5, P2=51.5, and P3=52.75. Averages of the number of leaves were P0=22, P1=110,P2=160, and P3=185. Averages of numbers of tillers were P0=7, P1=25, P2=37, and P3=38. Averages ofnew production (g) were P0=17.75, P1=123.5, P2=178, and P3=192.25. Averages of root length (cm)were P0=37.8, P1=39.5, P2=41.775, and P3=49.5. Fertilization with natural fertilizer from goat manurehas a genuine effect (P<0.01) on the growth of Beha grass. The best dosage of fertilization with goatmanure was 15 ton/ha.

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UJI PENGGUNAAN BERBAGAI MEDIA TUMBUH DAN PEMBERIAN PUPUK GROWMORE PADA AKLIMATISASI TANAMAN ANGGREK Dendrobium

Jurnal Ilmiah Pertanian ◽

10.31849/jip.v15i2.1960 ◽

2019 ◽

Vol 15 (2) ◽

pp. 105-114

Author(s):

GUSTI MARLINA ◽

MARLINDA MARLINDA ◽

HENI ROSNETI

Keyword(s):

Plant Height ◽

Growth Media ◽

Growing Media ◽

Randomized Design ◽

Honestly Significant Difference ◽

Significant Difference ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Difference Test ◽

Honestly Significant Difference Test

The research objective was determine the effect of various growth media and growmore fertilizer on the acclimatization of Dendrobium orchids. study used a Completely Randomized Design (CRD) Factorial consisting of 2 factors, the first is (M) of growing media which consists of 3 levels, namely: M1 (medium fern root), M2 (wood charcoal medium), M3 (coir media coconut) and the second is (G) growmore fertilizer which consists of 4 levels, namely: G0 (without Growmore fertilizer), G1 (Growmore fertilizer 2 g / liter), G2 (fertilizer Growmore 4 g / liter), G3 (Growmore fertilizer 6 g / liter). Determine the growth of orchid plants, observations were made with the following parameters Growth Percentage (%), Plant Height (cm), Number of leaves (strands), and Number of tillers (stems). Data obtained were analyzed statistically, then continued with the Honestly Significant Difference Test (BNJ) at the level of 5%. The results showed that the single treatment of the use of growing media did not have a significant effect on the parameters of observation of growth percentage (%), plant height (cm), number of leaves (strands), and number of tillers (stems). The growth of growmore fertilizer also did not show a significant effect on the parameters of observation of growth percentage (%), plant height (cm), number of leaves (strands), and number of tillers (stems). Interaction of the use of various growth media and growmore fertilizer did not show a significant effect on the percentage of growth (%), plant height (cm), number of leaves (strands), and number of tillers (stems).

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Respon pertumbuhan rumput Setaria (Setaria spachelata) yang diberikan pupuk kotoran satwa Kuskus asal penangkaran

Jurnal Sains dan Teknologi Peternakan ◽

10.31605/jstp.v2i2.1040 ◽

2021 ◽

Vol 2 (2) ◽

pp. 74-81

Author(s):

Fredrik R.S. Mauri ◽

Diana Sawen ◽

Alnita Baaka

Keyword(s):

Plant Height ◽

Growth Response ◽

Organic Fertilizer ◽

Animal Manure ◽

Forage Plants ◽

Setaria Sphacelata ◽

Number Of Leaves ◽

Agricultural Plants ◽

And Control ◽

Food Consumed

Cuscus is an arboreal animal whose habitat is in the forest with the type of food consumed are fruits, leaf shoots, and agricultural plants. Animal manure can also be used as organic fertilizer for forage plants. This study aims to determine the growth response of Setaria grass (Setaria sphacelata) given a dose of cuscus manure fertilizer with banana and avocado consumption. The study was designed in a CRD with 3 treatments. The treatments were P0 = without fertilizer (100% soil), P1 = 40 g/polybag of cuscus manure with banana consumption, and P2 = 40 g/polybag of cuscus manure with avocado consumption. Planting is done by pols on polybags measuring 30 x 25 cm. The results showed that the highest plant height of Setaria grass was found in P2 with an average of 101.70, then P1 was 101.47 and P0 was 71.16 cm/week. The results were the same for the number of leaves and tillers, P2 showed significantly higher results (P<0.05), followed by P1 and control. The application of organic fertilizer of cuscus manure with the consumption of bananas and avocados can increase the growth of Setaria grass.

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ALTERNATIVE SUBSTRATES FOR PRODUCTION OF YELLOW PASSION FRUIT SEEDLINGS

JOURNAL OF NEOTROPICAL AGRICULTURE ◽

10.32404/rean.v7i1.3890 ◽

2020 ◽

Vol 7 (1) ◽

pp. 43-48

Author(s):

Eduardo Santana Aires ◽

Carlos Alberto Aragão ◽

Itala Laiane Silva Gomes ◽

Gilmário Noberto de Souza ◽

Isa Gabriela Vieira de Andrade

Keyword(s):

Plant Height ◽

Dry Matter ◽

Passion Fruit ◽

Dry Matter Accumulation ◽

Alternative Substrates ◽

Randomized Design ◽

Length Width ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Yellow Passion Fruit

This study aimed to evaluate different substrates to produce yellow passion fruit seedlings. For this purpose, an experiment was carried out in a greenhouse, from April to June 2016. A completely randomized design with five replications, in a 6 x 5 factorial scheme was used. The first factor was the alternative substrates (poultry, cattle and equine manure, sugarcane and coconut bagasse, and Organomais compost). The second factor was the replacement levels to the commercial substrate of Plantmax® (100:0, 75:25, 50:50, 25:75, 0:100). The seedling evaluation was carried out 42 days after sowing. The following variables were analyzed: length, width, and the number of leaves; plant height and shoot dry matter. A significant effect (P0.05) for the interaction between the factors was verified. The addition of 60% cattle manure and Organomais to the commercial substrate Plantmax® promoted greater length, width, and the number of leaves, plant height, and shoot dry matter accumulation in the yellow passion fruit seedlings.

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Pengaruh Pemberian Pupuk Organik Cair Nasa Dan Pupuk Kompos Terhadap Pertumbuhan Tanaman Kenaf ( Hibiscus cannabinus L)

Agrifarm : Jurnal Ilmu Pertanian ◽

10.24903/ajip.v7i1.364 ◽

2018 ◽

Vol 7 (1) ◽

pp. 6

Author(s):

Hairin Juanda ◽

Tutik Nugrahini ◽

Mahdalena Mahdalena

Keyword(s):

Plant Growth ◽

Leaf Area ◽

Plant Height ◽

Analysis Of Variance ◽

Organic Fertilizer ◽

Hibiscus Cannabinus ◽

Stem Diameter ◽

Randomized Design ◽

Number Of Leaves ◽

Completely Randomized Design

Effect of NASA Liquid Organic Fertilizer and Compost Fertilizer on Kenaf Plant Growth (Hibiscus cannabinus L). This study aims to determine the effect of NASA POC, compost fertilizer and the interaction of NASA POC and compost fertilizer on kenaf plants. This research was carried out at East Borneo Samarinda Indonesia. with a time of ± 4 months, starting from June to September 2016. The design of the study uses factorial completely randomized design (CRD) consisting of 2 treatments and 4 replications. The treatment consists of 2 factors. The first factor is the administration of NASA POC consisting of 4 levels, namely: j0: Control (without treatment), j1: POC with a concentration of 10 ml / liter of water, j2: POC with a concentration of 15 ml / liter of water, j3: POC with a concentration of 20 ml / liter of water. The second factor is Compost fertilizer consisting of 4 levels, namely: k0: Without the provision of Compost Fertilizer, k1: Composting 500 gram / polybag, k2: Composting Fertilizer 700 grams / polybag, k3: Composting 800 grams / polybag. From the analysis of variance showed that the effect of giving NASA POC (J), compost (K) and the interaction of NASA POC and compost (JK) did not affect the parameters of plant height 30 DAP, 60 DAP and 90 DAP, stem diameter 30 DAP, 60 DAP and 90 DAP, number of leaves 30 DAP, 60 DAP and 90 DAP, leaf area 30 DAP, 60 DAP and 90 DAP.

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ADDITION OF THIAMIN AND LEAF FOLIAR IN ABACA BANANA (Musa textillis Nee.) ACCLIMATIZATION

Agrivet ◽

10.31315/agrivet.v25i2.4286 ◽

2021 ◽

Vol 25 (2) ◽

pp. 88

Author(s):

Rina Srilestari ◽

Suwardi Suwardi

Keyword(s):

Raw Materials ◽

Paper Industry ◽

Economic Value ◽

Large Space ◽

Planting Material ◽

Randomized Design ◽

Number Of Leaves ◽

The Right ◽

Leaf Fertilizer ◽

Range Test

The economic value of the Abaca banana is on the trunk which contains fiber for valuable textile and paper industry raw materials. The multipurpose fiber and its prospects are quite good, so the abaca plant gets a lot of attention from various community groups both private, state-owned, cooperative and farmers. Propagation through multiplication humps is very low, planting material is easily damaged in transportation, is not durable and requires large space so that transportation costs are high. One alternative to overcome the provision of healthy, large and fast seedlings is to use seedlings from tissue culture. The aim of the study is to get the right thiamin and leaf fertilizer concentrations at the acclimatization stage. The experimental design used was a 2-factor Complete Randomized Design, Thiamin concentration (1 ml / l, 2 ml / l, 3 ml / l) and leaf fertilizer (0.5 ml / l, 1 ml / l, 1.5 ml / l). The data obtained were analyzed for diversity at 5% level and further tests using Duncan Multiple Range Test (DMRT) level of 5%. The results showed no interaction between thiamin and leaf fertilizer, Thiamin 2 ml / l gave the best results on all parameters and 1.5 ml / l leaf fertilizer could increase plant height and the number of leaves of abaca banana plants.Keywords: Thiamin, leaf fertilizer, abaca banana, acclimatization

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Kajian Penggunaan Biourine Sapi dan Ekstrak Bawang Merah Terhadap Sifat Agronomi Tanaman Kubis (Brasicca oleracea L.) Dataran Rendah

Agrifarm : Jurnal Ilmu Pertanian ◽

10.24903/ajip.v7i2.434 ◽

2019 ◽

Vol 7 (2) ◽

pp. 63

Author(s):

Tutik Nugrahini ◽

Asiah Wati ◽

Dwi Indriani

Keyword(s):

Plant Height ◽

Block Design ◽

Economic Value ◽

Brown Sugar ◽

Onion Extract ◽

East Kalimantan ◽

Randomized Block Design ◽

Number Of Leaves ◽

Growing Conditions ◽

Control Water

Cabbage (Brasicca oleracea L.) is one type of vegetable plant from the Brassicaceae family. This plant is a source of nutrients that contain nutrients, vitamins, minerals and fiber for the human body. And has economic value. Cabbage cultivation is influenced by the types of cultivated varieties that are in accordance with growing conditions. In general, cabbage plants grow on highlands. Along with advances in plant breeding technology, cabbage plants are available that can adapt to the lowlands and technically attempt to increase production by fertilizing. The study aimed to determine the effect of beef biourine, red onion extract and beef biourine and red onion extract on the agronomic properties of cabbage plants. The study was conducted for 4 months, from February to June 2018. The location of the study was carried out in the Sempaja Timur Village, Samarinda City, East Kalimantan Province. cabbage seeds, top soil, manure, husks, NPK fertilizer, cow urine, empon-empon, soybeans, brown sugar, shallots. The design used in this study is Factorial Randomized Block Design (RBD) with 2 treatments. The first treatment was beef biourine (S) with 4 levels, namely with 3 replications: s0: control (water), s1: 25% concentration of cow biourine, s2: 75% concentration of bovine biourine, s3: 100% concentration of bovine biourine and second treatment red onion extract (B) with 4 levels, namely: b0: control (water), b1: 25% concentration of red onion extract, b2: 75% concentration of red onion extract, b3: 100% concentration of shallot extract. The results of research on bovine biourine influenced plant height, number of leaves at ages 7, 14, 21, 28.35 and 42 days after planting. The weight of the crop with the best results at the level of b2 (75% biourine cow) weighing 596.58 grams, onion extract affects plant height, number of leaves at 7, 14, 21, 28.35 and 42 hst, crop weight with the best results at the m2 level (75% red onion extract) with a weight of 567.14 grams and the interaction of the treatment of beef biourine and shallots affects plant height, number of leaves at the ages of 7, 14, 21, 28.35 and 42 days and days formed at the level b2m2 (75% beef biourine and 75 red onion extracts).

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Toleransi Plasma Nutfah Padi Lokal terhadap Salinitas

Buletin Plasma Nutfah ◽

10.21082/blpn.v23n1.2017.p51-58 ◽

2018 ◽

Vol 23 (1) ◽

pp. 51 ◽

Cited By ~ 1

Author(s):

Tintin Suhartini ◽

Try Zulchi

Keyword(s):

Plant Height ◽

Salinity Tolerance ◽

Negative Correlation ◽

Dry Weight ◽

Gene Bank ◽

Dead Leaf ◽

Randomized Design ◽

Highly Sensitive ◽

Number Of Leaves ◽

Completely Randomized Design

AbstractThe research was aimed to screen the local rice germplasms for salinity tolerance. The 104 local rice germplasms were derived of collection of Gene Bank of Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD) were tested to salinity. The activities was carried out in greenhouse of ICABIOGRAD. The 15 day old seedlings were planted in pots soil contained NaCl 0.4%. One accession of rice was planted in 2 pots, each pot contained 4 seedlings. Observation of plant symptom was conducted after 1 month planted. The experiment was carried out in a Completely Randomized Design with 2 replications. The results of the analysis showed there were different responsses among rice accesions to the treatment. Salinity caused dry and dead leaves, there were very significant differences between the varieties for plant height, dry weight plant, number of total leaves, and number of green leaves. There were a high and negative correlation of plant height and total number of leaves with percentage of dead leaves. The result showed 21 accesions were tolerant to moderately&#8722;tolerant, while Pokkali as a tolerant check, most tolerant. There were two accessions were tolerant with dead leaf percentage <50%, i.e. Tjempo Brondol (reg. 5800) and Gembira Putih (reg. 20602), whereas Pokkali as resistant check had low dead leaf percentage (16.9%) and 19 accessions were moderately-tolerant with dead leaf percentage <70%, and 82 accesions were sensitive to highly-sensitive.AbstrakPenelitian ini bertujuan untuk menyaring plasma nutfah padi yang memiliki sifat toleran terhadap salinitas. Sejumlah 104 plasma nutfah padi lokal yang berasal dari koleksi Bank Gen BB Biogen diuji terhadap salinitas. Kegiatan dilakukan di rumah kaca BB Biogen. Bibit padi berumur &plusmn;15 hari ditanam pada pot berisi tanah yang sudah dilumpurkan dengan larutan garam NaCl 0,4% (4.000 ppm). Satu aksesi padi ditanam pada 2 pot, masing masing pot berisi 4 tanaman. Setelah berumur 4 minggu dari tanam dilakukan pengamatan meliputi tinggi tanaman, bobot kering akar, bobot kering tanaman, jumlah daun total, jumlah daun hijau, dan jumlah daun mati. Pengujian menggunakan Rancangan Acak Lengkap dengan 2 ulangan. Hasil analisis varians menunjukkan terdapat perbedaan respons plasma nutfah padi terhadap perlakuan salin yang diberikan. Perbedaan sangat nyata terdapat pada tinggi tanaman, bobot kering tanaman, jumlah daun total, dan jumlah daun hijau. Terdapat korelasi yang tinggi dan negatif pada tinggi tanaman dan jumlah daun total dengan persentase daun mati. Hasil pengujian telah terpilih 21 aksesi plasma nutfah padi toleran hingga agak toleran, sedangkan Pokkali sebagai cek sangat toleran. Dua aksesi yang toleran dengan persentase daun mati <50%, yaitu Tjempo Brondol (reg. 5800) dan Gembira Putih (reg. 20602), Pokkali sebagai varietas kontrol dengan tingkat persentase daun mati 16,9%, sedangkan 19 aksesi termasuk kelompok agak toleran dengan persentase daun mati <70%, dan 82 aksesi termasuk kelompok peka hingga sangat peka.

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