scholarly journals Prevalence of gastrointestinal endoparasites in Waringin sheep (Ovis aries) fed with complete forage and forage-concentrate

2021 ◽  
Vol 912 (1) ◽  
pp. 012097
Author(s):  
R Azlan ◽  
M Tanjung
Keyword(s):  
Lower Number ◽  
Glass Beads ◽  
Ovis Aries ◽  
High Rate ◽  
Microscopical Examination ◽  
Feed Composition ◽  
Substantial Impact ◽  
Helminth Eggs ◽  
Sedimentation Technique ◽  
North Sumatra

Abstract Feed composition has a substantial impact on the persistence of gastrointestinal endoparasite infection in sheep. This study aimed to compare the prevalence of gastrointestinal endoparasites in Waringin sheep (Ovis aries) fed with complete forage and feed concentrate in Sidomulyo Village, Langkat Regency, North Sumatra. The study has been conducted from June to August 2020 with a total of 20 sheep for each type of feed. Fecal samples from each sheep were prepared for microscopical examination using sedimentation technique (glass beads). Gastrointestinal endoparasites was identifiedmorphologically. The results documented 3 genera of endoparasites namely Haemonchus (Nematoda), Fasciola (Trematoda) and Paramphistomum (Trematoda). Based on the type of forages, sheep fed with forage-concentrate showed a lower number of helminth eggs than the complete forage group. Haemonchus sp was identified as the most frequent parasite in both type of feeds. The intensity was categorized from mild-to-moderate level of infection. Provision of forage-concentrate was then proven to prevent a high rate of infection in Waringin sheep.

Viability of nematode eggs in high rate algal ponds: the effect of physico-chemical conditions

2000 ◽  
Vol 42 (10-11) ◽  
pp. 371-374 ◽  
Author(s):  
S. Araki ◽  
J. M. González ◽  
E. de Luis ◽  
E. Bécares
Keyword(s):  
Hydraulic Retention Time ◽  
Pilot Scale ◽  
High Rate ◽  
Sterile Water ◽  
Egg Viability ◽  
High Rate Algal Ponds ◽  
Helminth Eggs ◽  
Physico Chemical ◽  
Nematode Eggs ◽  
Chemical Conditions

The viability of Parascaris equorum eggs was studied in two experimental pilot-scale high-rate algal ponds (HRAPs) working in parallel with 4 and 10 days hydraulic retention time respectively. Semi-permeable bags of cellulose (15000 daltons pore size) were used to study the effect of physico-chemical conditions on the survival of these helminth eggs. Three thousand eggs were used in each bag. Replicates of these bags were submerged for 4 and 10 days in the HRAPs and egg viability was compared with that in control bags submerged in sterile water. After 4 days exposure, 60% reduction in viability was achieved, reaching 90% after 10 days, much higher than the 16% and 25% found in the control bags for 4 and 10 days respectively. Ionic conditions of the HRAP may have been responsible for up to 50–60% of the egg mortality, suggesting that mortality due to the ionic environment could be more important than physical retention and other potential removal factors.

scholarly journals Primitive techno-economic study of bio-diesel and bio-active compound production from microalgae

2020 ◽  
Vol 44 (1) ◽  
Author(s):  
Sayeda M. Abdo ◽  
Guzine I. El Diwani ◽  
Kamel M. El-Khatib ◽  
Sanaa A. Abo El-Enin ◽  
Mohammed I. El-Galad ◽  
...  
Keyword(s):  
Active Compound ◽  
Municipal Wastewater ◽  
Scenedesmus Obliquus ◽  
Cetane Number ◽  
Treatment Plant ◽  
Acid Methyl Ester ◽  
Biodiesel Production ◽  
High Rate ◽  
Total Phenolic ◽  
Microscopical Examination

Abstract Background Microalgae cells can be identified as a potential source for new and renewable energy. The economic investigation for biodiesel and bio-active compound production from the microalgae community (Bloom), which are collected from the high rate algal pond (HRAP) constructed to treat municipal wastewater at Zenin wastewater treatment plant, Giza, was the main target of study. Results The microscopical examination showed that Scenedesmus obliquus is the dominant species. The total carotenoids were extracted using jojoba oil and determined by high-performance liquid chromatography (HPLC) to reach 81.44 μg/g. The biodiesel production through acid transesterification reaction recorded 70.6% of fatty acid methyl ester content with high cetane number (44) and low acid value. Such results prove that the obtained biodiesel has better ignition quality. The total phenolic and flavonoid compounds have been derived from the remaining biomass to give 5.36 ± 0.03 and 1.50 ± 0.19 mg/g respectively. Finally, total proteins and carbohydrates content in algal cells were recorded 54.3 and 1.5 mg/g successively Conclusion The preliminary economic evaluation showed that the production of biodiesel and carotenoids from the microalgae growing in municipal wastewater can be considered, as a techno-economic feasible process.

UTILIZATION OF UNIFORMLY LABELLED 14C-GLUCOSE IN THE PINEAL BODY OF GOATS

1961 ◽  
Vol 38 (3) ◽  
pp. 353-360 ◽  
Author(s):  
Bo Hellman ◽  
Stig Larsson
Keyword(s):  
Amino Acids ◽  
High Rate ◽  
Pineal Body ◽  
Chromatographic Technique ◽  
Microscopical Examination ◽  
Age Group ◽  
Wet Weight ◽  
Parenchymal Cells ◽  
Very High

ABSTRACT The in vitro utilization of uniformly 14C-labelled glucose was studied in the pineal body of goats by using a quantitative application of the radio paper-chromatographic technique. The O2 consumption as well as the formation of CO2 and lactic acid from glucose in the incubation medium was comparatively very high in young goats and decreased gradually with increasing age. The same was true for the formation of labelled amino acids. Thus, there were no measurable amounts of radioactive amino acids in goats older than 6 years, while in the animals 1–3 months old no less than 11.7 μg glucose was converted into amino acids per 25 mg wet weight of the pineal body Glutamic acid and alanine were found in the highest amounts among the different amino acids formed from glucose in the youngest age group. There were also appreciable amounts of arginine, glutamine, δ-aminobutyric acid and aspartic acid. Microscopical examination revealed that not only progressive degenerative changes but also a markedly reduced number of parenchymal cells per unit volume, might account for the diminished glucose metabolism found in the pineal body of adult goats. The metabolic findings, especially the very high rate of formation of amino acids from glucose in the youngest animals, are discussed in the light of the result of recent investigations, which suggest secretion of a protein hormone from the non-adult pineal body.

Technical validation of PD-L1 SP142 assay for TNBC in a large academic center: Unexpected challenges.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e15253-e15253
Author(s):  
Sharon Nofech-Mozes ◽  
Elzbieta Slodkowska
Keyword(s):  
Inflammatory Cells ◽  
High Rate ◽  
Substantial Impact ◽  
Borderline Cases ◽  
Academic Center ◽  
Need To Evaluate ◽  
Technical Validation ◽  
Tumor Edge ◽  
Potential Issue

e15253 Background: The IMpassion 130 trial reported that 41-46% of TNBC are positive for PD-L1 SP142 based on centralized testing. While the FDA approved it as a companion diagnostic assay with cut off value of 1%, there are no published guidelines for technical validation in local laboratories. Furthermore, with the need to evaluate staining only in inflammatory cells (IC) the readout becomes particularly challenging for cases around the cutoff point. We report our prevalence of PD-L1 positivity and proportion of borderline cases in our validation. Methods: We ran PDL1 SP142 using the recommended protocol from Roche on the following groups: G1 - 53 consecutive TNBC cases (excisions tumors ≥7mm), G2 - 10 consecutive TNBC cases (breast specimens only) that would have met the inclusion criteria for the trial (LABC with poor NAT response or metastatic), G3 – proficiency testing slide from an EQA provider (4 cores of TNBC 1 negative, 1 borderline low positive and 2 high positive). All cases were read by 2 breast pathologists that received training on PDL1 readout by Roche (one – in class and online, one – online). Results: Borderline staining around the 1% cutoff (11 of 63) and low positive (9 of 63) were encountered in the validation sets more often than shown in training sessions. Classification of borderline cases may have a substantial impact on the rate of PDL1 positivity that potentially could approach 75%. Borderline cases often have IC staining either at the advancing tumor edge, as small collections of highly positive IC or around benign ducts entrapped within the tumor. Conclusions: The prevalence of PDL1 positive TNBC in all comers could be higher than observed in IMpassion 130 (at least 50-60%). Pathologist training in readout is of utmost importance due to high rate of borderline/low positive cases raising the need for central testing in borderline cases. Guideline recommendations for technical validation similar to other class II biomarkers would be useful. Studies on interobserver variability are needed to further address this potential issue. [Table: see text]

scholarly journals High Rate of Bleeding and Arterial Thrombosis in COVID-19: Saudi Multicenter Study

2020 ◽  
Author(s):  
Abdulrahman Al Raizah ◽  
Ahmed AlAskar ◽  
Naila Shaheen ◽  
Khalid Aldosari ◽  
Mohamed Alnahdi ◽  
...  
Keyword(s):  
Multicenter Study ◽  
Hospitalized Patients ◽  
High Rate ◽  
Bleeding Complications ◽  
Controlled Study ◽  
Similar Degree ◽  
Thrombotic Complication ◽  
Substantial Impact ◽  
Composite Events ◽  
D Dimer

Abstract Background: Coronavirus disease 2019 (COVID-19) is a global pandemic with a substantial impact on mortality, the health system and the economy8,9. Several observational studies reported the rate of venous and arterial thrombotic events in patients infected with COVID-19, with conflicting results . The aim of this multicentre study was to estimate the rate of thrombotic and bleeding events in hospitalized patients diagnosed with COVID-19 in Saudi Arabia. Method: multicenter study of 636 hospitalized patients with COVID-19.Result: Twelve patients were diagnosed with VTE 1.89% (95% CI, 1.18–3). The rate in the non-ICU group was 0.19% (95% CI, 0.04–0.84) compared to 10.38% (95% CI, 6.45–16.27) in the ICU group. Fourteen patients were diagnosed with an arterial event with an overall rate of 2.20% (95% CI, 1.43–3.38). The rate in the non-ICU group was 0.94% (95% CI, 0.46–0.1.93) and 8.49% (95% CI, 5.01–14.04) in the ICU group. The overall composite events rate was 2.99% (95% CI, 2.06–4.31). The composite events rate in the non-ICU group was 0.94% (95% CI, 0.46–0.1.93) and 13.21% (95% CI, 8.7–19.54) in the ICU group. Eleven patients developed bleeding with an overall rate of 1.73% (95% CI, 1.06–2.81). The bleeding rate in the non-ICU group was 0.19% (95% CI, 0.04–0.84), and 9.43% (95% CI, 5.72–15.16) in the ICU group. Of the selected risk factors, the only risk factor that predicted VTE and the composite outcome, was the baseline D-dimer. (OR 1.31, 95% CI, 1.084-1.573, p=0.005) and composite events (OR 1.32, 95% CI, 1.126-1.555, p=0.0007).Conclusion: Of 636 adults with COVID-19, the rate of VTE was similar to the rate of hospitalized patients with a similar degree of critical illness. In contrast to the risk of VTE, we found a high rate of arterial and bleeding complications in patients admitted to ICU. An elevated D-dimer at baseline could predict a thrombotic complication in the COVID-19 patients, which may assist in the identification of these patients. Given the high rate of bleeding, the current study suggests that the intensification of anticoagulation in COVID-19 patients beyond the standard of care, should be pursued with caution and is best evaluated in a randomised controlled study.

scholarly journals Short Communication: Strong genetic differentiation of the endemic rosin-producing tree Styrax sumatrana (Styracaceae) in North Sumatra, Indonesia

2017 ◽  
Vol 18 (4) ◽  
pp. 1331-1335 ◽  
Author(s):  
HENTI HENDALASTUTI RACHMAT ◽  
ARIDA SUSILOWATI ◽  
DENI ELFIATI ◽  
KANSIH SRI HARTINI ◽  
WIZA NONI FARADILLAH
Keyword(s):  
Genetic Differentiation ◽  
Nucleotide Diversity ◽  
Short Communication ◽  
Forest Degradation ◽  
High Rate ◽  
Genetic Structuring ◽  
Mountain Ranges ◽  
Cpdna Region ◽  
Population Structuring ◽  
North Sumatra

Rachmat HH, Susilowati A, Elfiati D, Hartini KS, Faradillah WN. 2017. Short Communication: Strong genetic differentiation of the endemic rosin-producing tree Styrax sumatrana (Styracaceae) in North Sumatra, Indonesia. Biodiversitas 18: 1331-1335. Styrax sumatrana is an economically important rosin-producing tree endemic to North Sumatra, Indonesia. Distribution of this species is very limited, and the high rate of forest degradation in Sumatra is increasing the necessity for conservation. To quantify genetic variation and population structuring, we collected individuals from 3 populations namely Pakpak Bharat, Humbang Hasundutan (Humbahas) and Tapanuli Utara in which each of the population was represented by 10 individuals. However, the successful rate of amplification was varied among populations and for later analysis, we only took an account those sequences showing clear electropherograms and disposed those which showed ambiguity. We sequenced trnL-trnF chloroplast DNA (cpDNA) region yielded 941 bp after alignment. The trnL-trnF assigned the species into 4 haplotypes in which Pakpak Bharat was differentiated significantly and not shared any similar haplotypes with two others populations. Humbahas and Tapanuli Utara was shared one common haplotype. Mean nucleotide diversity at silent sites ranged 0 - 3.33 x 10-3, while nucleotide diversity at non-synonymous site ranged 0 - 5.9 x 10-4. Strong genetic differentiation was also found among 3 origin populations, with the highest pairwise genetic differentiation found on Pakpak Bharat and Tapanuli Utara (FST= 0.80952). Clear and apparent genetic structuring was possibly caused by geographical barriers such as highland and mountain ranges (Bukit Barisan mountain ranges) which acted as effective barriers to gene flow among population. The findings suggest that conservation efforts should focus on every population because each of the population maintains distinct genetic identity.

scholarly journals Identifikasi dan Konsep Pengembangan Kawasan Ruang Terbuka Hijau (RTH) Kota Padangsidimpuan

Arsitektura ◽  
2020 ◽  
Vol 18 (2) ◽  
pp. 337
Author(s):  
Yunita Syafitri Rambe
Keyword(s):  
Urban Space ◽  
Urban Areas ◽  
Quantitative Methods ◽  
High Rate ◽  
Open Spaces ◽  
Qualitative And Quantitative ◽  
Qualitative And Quantitative Methods ◽  
Interview Survey ◽  
North Sumatra ◽  
Public Open Spaces

<p class="Abstract"><em>Urban areas in Indonesia tend to experience a typical problem, namely the high rate of population growth due to urbanization which causes the management of urban space to become increasingly difficult. The high and increasing number of urban population has implications for the high pressure on the use of urban space, so that it is necessary to organize the space in urban areas, especially public and social facilities as well as public open spaces in urban areas. Padangsidimpuan City is one of the cities in North Sumatra Province that has green open spaces but has not received special attention. So it is necessary to do research on identification and development. This is done with the aim of creating a comfortable, productive and sustainable urban space to create a sustainable existence of public open spaces in urban areas. The method used in this research is qualitative and quantitative methods with literature, interview, survey and recording techniques which later the data obtained will be analyzed for the development of green open spaces (RTH). It is hoped that the results of this research will be useful as a basic foundation for interested parties in developing and in further research.</em></p>

scholarly journals Trematodiasis occurrence in cattle along the Progo River, Yogyakarta, Indonesia

2019 ◽  
Vol 12 (4) ◽  
pp. 593-597 ◽  
Author(s):  
Korbinianus Feribertus Rinca ◽  
Joko Prastowo ◽  
Dwi Priyo Widodo ◽  
Yudhi Ratna Nugraheni
Keyword(s):  
Infection Rate ◽  
High Rate ◽  
Multiple Infections ◽  
Trematode Species ◽  
Infection Rates ◽  
Fecal Samples ◽  
Sedimentation Technique ◽  
High Infection ◽  
Fasciola Spp ◽  
Trematode Parasites

Aim: This study aimed to measure the occurrence of trematodiasis in cattle along the Progo River, a district of Yogyakarta, Indonesia. The findings help to establish the magnitude of the disease and encourage prevention and treatment of this condition. Materials and Methods: Trematode eggs were extracted from 100 fecal samples collected from cattle. The eggs were examined using the sedimentation technique, and the method of Parfitt and Banks was used to differentiate Paramphistomum spp. eggs from Fasciola spp. eggs. Results: The infection rate of trematode parasites was 50%. Cattle experienced multiple infections of both Paramphistomum spp. and Fasciola spp., as well as single infections of one species or the other. All breeds were vulnerable to infections of both trematode species, although different cattle breeds, including Peranakan Ongole crossbreeds, Simmental crossbreeds, and Limousin crossbreeds, showed differences in infection rate. The highest rate of infection with Paramphistomum spp. (15.78%) occurred in the Simmental crossbreeds. The highest rate of infection (31.57%) with Fasciola spp. was in the Peranakan Ongole crossbreeds. Multiple infections of both Paramphistomum spp. and Fasciola spp. were highest in Simmental crossbreed cattle (28.97%). Conclusion: The high infection rates of trematode parasites found in fecal samples, particularly of Fasciola spp., indicate that the cattle along the Progo River in Indonesia experience a high rate of trematodiasis disease.

scholarly journals Pengaruh Konsumsi Biskuit Katuk , Dan Pijat Oksitosin Terhadap Keberhasilan Pemberian Asi Eksklusif Pada Ibu Menyusui Di Puskesmas Simalingkar Tahun 2019

2019 ◽  
Vol 10 (2) ◽  
pp. 856-866
Author(s):  
Srilina Br Pinem ◽  
Astaria Br Ginting ◽  
Lasria Simamora
Keyword(s):  
Breast Milk ◽  
Exclusive Breastfeeding ◽  
Health Workers ◽  
High Rate ◽  
A Value ◽  
Before And After ◽  
Post Test ◽  
Quasi Experimental ◽  
Relationship Of ◽  
North Sumatra

Latar Belakang: Tingginya  angka  stunting  dan  AKB  di  Indonesia  merupakan  masalah  nasional.  Khususnya  di Sumatera Utara. ASI merupakan makanan yang paling tepat untuk mengatasi kedua masalah tersebut. Faktor utama ibu tidak memberikan ASI kepadaanaknya dikarenakan ibu berasumsi ASI yang diberikan tidak cukup dan produksi ASI tidak lancar hal ini terlihat dari capaian nasional pemberian ASI ekslusif masih jauh dari target 80 % .Tujuan: Tujuan penelitian ini untuk mengetahui pengaruh konsumsi biskuit katuk terhadap keberhasilan pemberian ASI Eksklusif pada ibu menyusui di Puskemsmas Simalingkar Tahun 2019.Metode: Jenis Penelitian yang digunakan adalah quasi eksperimen dengan design pre test post test one group design. Hasil:  Hasil penelitian menunjukkan nilai p= 0,000 dimana terdapat hubungan tidur tenang 2 jam akibat pemberian biskuit  katuk dan pijat oksitosin dengan pemberian ASI eksklusif, dan menunjukkan   nilai p=0,000 artimya terdapat hubungan penuruann berat badan 8%  akibat pemberian biscuit  katuk dan pijat oksitosin dengan pemberian ASI eksklusif. menunjukkan bahwa nilai p=0,000 artimya terdapat perbedaan tidur tenang sebelum dan sesudah diberi biskuit katuk dan pijat oksitosin. Terjadi peningkatan tidur tenang 2 jam setelah diberikan biskuit katuk dan pijat oksitosin setelah  hari  keempat.Simpulan: Sehingga dapat disimpulkan pemberian biskuit katuk dan pijat oksitosin efektif dalam kelancaraan pemberian ASI yang mendukung keberhasilan pemberian ASI Eksklusif. Diharapkan kepada tenaga kesehatan untuk memberikan motivasi kepada ibu untuk mengkonsumsi biskuit katuk pada ibu nifas. Kata Kunci: Katuk, ASI, Pijat Oksitosin Background: The high rate of stunting and IMR in Indonesia is a national problem. Especially in North Sumatra. Breast milk is the most appropriate food to overcome these two problems. The main factor of mothers not giving ASI to their children is because mothers assume that breast milk is not enough and production of ASI is not smooth, it can be seen from the national achievement of exclusive breastfeeding which is still far from the target of 80%.Objective: The purpose of this study was to determine the effect of katuk biscuit consumption on the success of exclusive breastfeeding in breastfeeding mothers in Puskemsmas Simalingkar in 2019.Method: This type of research is a quasi-experimental design with pre-test post test one group design.Results: The results showed a value of p = 0,000 where there was a relationship of calm sleep2 hours due to the administration of cough biscuits and oxytocin massage with exclusive breastfeeding,and showed a value of p = 0,000. exclusive breastfeeding. shows that p = 0,000 means thatthere is a difference in calm sleep before and after being given a katuk biscuit and oxytocin massage. An increase in calm sleep 2 hours after given katuk biscuits and oxytocin massage after the fourth day.Conclusion: So it can be concluded that the administration of katuk biscuits and oxytocin massage is effective in the smoothness of breastfeeding which supports the success of exclusive breastfeeding.It is expected that health workers will motivate mothers to consume katuk biscuits for postpartum mothers.Keywords: Katuk, ASI, Oxytocin Massage

scholarly journals Endoparasite infestation of Indian Muntjac (Muntiacus muntjak) in Citra Pesona Ladangku Animal Park, North Sumatra

2021 ◽  
Vol 912 (1) ◽  
pp. 012077
Author(s):  
S N Rahmawati ◽  
M Tanjung
Keyword(s):  
Glass Bead ◽  
Microscopical Examination ◽  
Intensity Of Infection ◽  
Fecal Samples ◽  
Indian Muntjac ◽  
Sedimentation Method ◽  
North Sumatra ◽  
Muntiacus Muntjak

Abstract An investigation on the infestation by endoparasites in the fecal samples of Indian Muntjac (Muntiacus muntjak) captived at Citra Pesona Ladangku Animal Park, North Sumatera, Indonesia had been conducted from October to November 2020. The study aimed to determine the endoparasite species, prevalence, and intensity of the infestation. Fecal samples were prepared for microscopical examination using glass bead sedimentation method. Based the observation on 9 deers, three species of endoparasites were found namely Ascaris sp, Haemonchus sp and Paramphistomum sp. Observations in the first week revealed the prevalence of each species from often (Ascaris sp, Haemonchus sp) to common (Paramphistomum sp) with light (Haemonchus sp, Paramphistomum sp) to moderate (Ascaris sp). Observations in the second week only revealed the presence of Paramphistomum with often prevalency and light intensity of infection.

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