scholarly journals Biotechnology methods to produce planting material of the genus Rubus L.

2021 ◽  
Vol 941 (1) ◽  
pp. 012027
Author(s):  
E N Raeva-Bogoslovskaya ◽  
O I Molkanova ◽  
I L Krakhmaleva ◽  
E V Soboleva
Keyword(s):  
Growth Regulator ◽  
Nutrient Medium ◽  
Indoleacetic Acid ◽  
Optimal Growth ◽  
Optimal Concentration ◽  
Plant Genotype ◽  
Clonal Micropropagation ◽  
Planting Material

Abstract The work is devoted to improving clonal micropropagation methods of the genus Rubus representatives. When cultivating R. arcticus L. cultivars, the optimal concentration of 6-benzylaminopurine (6-BAP) was 0.3 mg L−1. Significant effect of FeEDTA concentration in the nutrient medium on multiplication of R. idaeus L. cultivars was established. The interaction of auxin type and plant genotype was revealed during rooting of blackberry cultivars and raspberry-blackberry hybrids. The nutrient medium supplemented with 0.5 mg L-1 indoleacetic acid contributed to the highest percentage of rhizogenesis in R. arcticus representatives (82%). The optimal growth regulator for conservation of raspberry explants was 6-BAP at a concentration of 0.3 mg L-1. One of the most representative in vitro collections of Rubus cultivars has been created.

scholarly journals Clonal Micropropagation of Cranberry (Oxycoccus palustris Pers.)

2021 ◽  
Vol 51 (1) ◽  
pp. 67-76
Author(s):  
Sergey Makarov ◽  
Irina Kuznetsova ◽  
Mikhail Upadyshev ◽  
Sergey Rodin ◽  
Anton Chudetsky
Keyword(s):  
Survival Rate ◽  
Nutrient Medium ◽  
Average Length ◽  
Forest Products ◽  
Hybrid Form ◽  
Clonal Micropropagation ◽  
Planting Material ◽  
Total Growth

Introduction. The last decade saw a considerable increase in the demand for European cranberry planting material (Oxyccocus palustris Pers.) among consumers of non-timber forest products. Cranberry possesses high nutritional and medicinal value. Cultivars and hybrids of European cranberry prove extremely productive for plantation growth using the method of clonal micropropagation with revitalized planting material. Study objects and methods. The research featured European cranberry plants of the Dar Kostromy cultivar and its hybrid form 1-15-635. The study focused on the effect of various medications and growth regulators on the biometric profile of European cranberry and its adaptation to non-sterile conditions at all stages of in vivo clonal micropropagation. Results and discussion. During the introduction stage, the highest viability belonged to the explants treated with AgNO3 (95–96%) and Lizoformin 3000 (5%) as the main sterilizing solutions at a 10-min exposure and a 5% solution of Ecosterilizer (1:1) at a 20-min exposure (90–95%). During the micropropagation proper, the number, average length, and total growth of shoots increased as the concentration of cytokinin 2ip in the WPM 1/4 nutrient medium rose from 1.0 to 5.0 mg/L. At the stage of in vitro rooting, the maximal number, average length, and total growth of roots in regenerated plants for both cultivars were observed when Kornerost 5.0 mg/L was added to the WPM 1/4 nutrient medium. At the stage of adaptation to in vivo conditions, Micogel 0.2 mg/L contributed to the highest survival rate (94–100%). Conclusion. During clonal micropropagation in vitro, the biometric profile of European cranberry (Oxyccocus palustris Pers.) and its survival rate under non-sterile conditions in vivo proved to depend on various growth-regulating substances and their concentrations.

scholarly journals Clonal micropropagation of bog cranberry (Vaccinium oxycoccos L.) in the Udmurt Republic

2021 ◽  
Vol 22 (1) ◽  
pp. 57-66
Author(s):  
E. N. Cheremnykh ◽  
T. G. Lekontseva ◽  
A. V. Khudyakova ◽  
A. V. Fedorov
Keyword(s):  
In Vitro Culture ◽  
Nutrient Medium ◽  
Root Formation ◽  
Sphagnum Moss ◽  
Multiplication Factor ◽  
Clonal Micropropagation ◽  
Planting Material ◽  
Udmurt Republic

The paper presents the results of 2018-2019 research on improving the technology of growing planting material of bog cranberry (Vaccinium oxycoccos L.) of Krasa Severa, Severyanka, Virussaare varieties on the basis of in vitro. Studied was the effect of the concentrations of growth regulators in the composition of the nutrient medium according to Anderson's recipe on the reproduction and subsequent rooting of micro cuttings, as well as the duration of cultivation and adaptation of micro plants depending on partial pruning of shoots. It has been established that at the stage of introduction into in vitro culture, sterilization of explants with 33% hydrogen peroxide in an exposure of 5-8 minutes with washing in 5 portions of sterile distillate gives 60-80 % of viable shoots. The optimum phase of plant development for the successful introduction of in vitro culture is the swelling of buds. Cultivation of micro cuttings was carried out in a light room at a temperature of 25±2 °С, a photoperiod of 16 hours. The duration of each subculturing was 30-60 days. For the stage of actual micropropagation on Anderson's nutrient medium, an increase in the dose of cytokinin 6-benzylaminopurine (6-BAP) from 0.2 to 0.5 mg/l and an increase in the duration of cultivation from 30 to 60 days contributed to a significant increase in the multiplication factor on average for the tested cranberry varieties.According to the efficiency of micropropagation, the varieties Virussaare and Krasa Severa were distinguished – 9.3-12.0 pcs/stalk, respectively. At the rooting stage, the use of a root-forming reagent of indolyl-3-acetic acid (IUK) in doses of 0.2, 0.5 and 1.0 mg/l in the composition of Anderson's nutrient medium did not affect the quality of root formation and the length of shoots of Virussaare micro-plants. No significant varietal differences in the root-forming ability of microcuttings were found. The tendency of better rooting of micro cuttings was observed in the Virussaare variety (90.3 %) compared to the Severyanka (85.7 %) and Krasa Severa (79.3 %) varieties. Micro plants of the Krasa Severa cultivar were characterized by the longest shoots, the total number of roots was less, but their length was longer in comparison with other cultivars. For the adaptation stage, a substrate from a mixture of lowland peat and sphagnum moss was used (1:1). The efficiency of adaptation of micro plants of cranberry varieties when cutting the tip of the shoots was 100 %. Pruning of micro plants shoots contributed to the formation of more side shoots and better development of the aboveground part of the plants.

scholarly journals Producing Planting Material of Rubus arcticus L. by Clonal Micropropagation

2021 ◽  
pp. 89-99
Author(s):  
Sergey S. Makarov ◽  
◽  
Galina V. Tyak ◽  
Anton I. Chudetsky ◽  
Irina B. Kuznetsova ◽  
...  
Keyword(s):  
Nutrient Medium ◽  
Experimental Studies ◽  
Forest Products ◽  
The Arctic ◽  
High Yield ◽  
Nontimber Forest Products ◽  
Effect Analysis ◽  
Clonal Micropropagation ◽  
Planting Material

The article presents the results of experimental studies on various propagation methods of the Arctic bramble in the Kostroma region. Industrial cultivation of forest berry plantations is a possible effective solution to the problem of low profitability of using nontimber forest products, reduction of the wild berries resources and their productivity and quality, and reclamation of cutover peatlands. It is advisable to use high-yield varietal planting material to create such plantations. Special attention is paid to propagation and production of healthy planting material using culture of plant cells and tissues. Data on sterilization of explants when introduced in vitro are given. The highest efficiency of sterilization was observed when using a chlorine-free eco-sterilizer (the plant survival rate on the MS nutrient medium was 90–93 %). The effect analysis of the passage number of regenerated plants on the multiplication factor of the Arctic bramble varieties was carried out. The optimal concentrations of cytokinins at the stage of micropropagation are shown. The largest number of the Arctic bramble roots was observed when adding 1.0 mg/L of Indole-3-butyric acid (IBA) and 0.5 mg/L of Ecogel to the nutrient medium. The technological and agrotechnical operations performed during the cultivation of planting material of forest berry plantations are considered. Data on the coefficients of vegetative propagation of plants and their resistance to diseases, yielding capacity, and recultivation of cutover peatlands are given. The best planting material of the Arctic bramble are ball-rooted seedlings. Sawdust and sphagnum were used in the cultivation of this berry on the peatland. The economic efficiency of its cultivation with the method of clonal micropropagation was 358.2 %. For citation: Makarov S.S., Tyak G.V., Kuznetsova I.B., Chudetsky A.I., Tsaregradskaya S.Yu. Producing Planting Material of Rubus arcticus L. by Clonal Micropropagation. Lesnoy Zhurnal [Russian Forestry Journal], 2021, no. 6, pp. 89–99. DOI: 10.37482/0536-1036-2021-6-89-99

scholarly journals Effect of Light on Rhizogenesis of Forest Berry Plants during Clonal Micropropagation

2021 ◽  
pp. 520-528
Author(s):  
Sergey Makarov ◽  
Sergey Rodin ◽  
Irina Kuznetsova ◽  
Anton Chudetsky ◽  
Svetlana Tsaregradskaya
Keyword(s):  
In Vitro Rooting ◽  
Light Emitting ◽  
Clonal Micropropagation ◽  
Fluorescent Lamps ◽  
Planting Material ◽  
Medicinal Value ◽  
American Cranberry ◽  
Food Market ◽  
Spectral Ranges

Introduction. Forest berry plants are popular on the food market and in pharmacy for their high nutritional and medicinal value. Plantations of forest berry plants can proliferate on unused lands, including depleted peatlands. Clonal micropropagation is the most effective method for obtaining large quantities of high quality planting material. Light-emitting diodes are highly effective for clonal micropropagation. The research objective was to study the effect of different spectral ranges on the process of root formation of forest berry plants in vitro. Study objects and methods. The research featured regenerant plants of half-highbush blueberry, arctic bramble, American cranberry, European cranberry, lingonberry, and Kamchatka bilberry of different cultivars. A set of experiments made it possible to study the effect of lighting type on the growth and development of the root system of forest berry plants in vitro using white fluorescent lamps, white spectrum LED lamps, and LED lamps with a combination of white, red, and blue spectra at the in vitro rooting stage of clonal micropropagation. Results and its discussion. The largest number (3.4–14.6 pcs.) and the maximum total length (10.0–156.9 cm) of roots were observed under LED lamps with a combination of white, red, and blue spectra. The effect was by 1.1–2.8 and 2.0–4.5 times higher than in the case of white-spectrum LED lamps, and by 2.3–7.0 and 3.3–14.9 times than in the case of fluorescent lamps. Variety and shape proved to have no significant effect on biometric indicators. Conclusion. LED lamps had a positive effect on the process of rhizogenesis of forest berry plants during clonal micropropagation. They appeared to be more effective than fluorescent lamps. The combination of white, blue, and red spectra increased the biometric parameters of plants at the stage of in vitro rooting.

scholarly journals Micropropagation of sea buckthorn (Hippophae rhamnoides L.)

1995 ◽  
Vol 4 (5-6) ◽  
pp. 503-512
Author(s):  
Yingmou Yao
Keyword(s):  
Growth Regulator ◽  
Woody Plant ◽  
Average Rate ◽  
Root Nodules ◽  
Optimal Concentration ◽  
Sea Buckthorn ◽  
Adventitious Buds ◽  
Woody Plant Medium ◽  
Different Origins

A protocol for micropropagation of sea buckthorn was developed starting with shoot tips or meristems from plants up to 18 years old. Among the different media used, the best medium for both initiation and multiplication was the woody plant medium (WPM). 6-Benzylaminopurine (BAP) was the most suitable growth regulator with an optimal concentration of 0.10-0.25 mg/l for initiation and 0.4-1.0 mg/l for multiplication. On WPM medium with BAP, the average rate of multiplication in Erlenmeyer flasks was 3.3-4.0 shoots per explant per month and in test tubes 2.0-3.0 shoots. Moreover, most explants produced several to tens of adventitious buds which grew into shoots. Explants rooted spontaneously in the multiplication medium at a frequency of about 33%. With this method, explants of different origins have been successfully propagated in vitro; and rooted young plants which had developed root nodules were produced both in the greenhouse and in the field.

scholarly journals Use of growth regulators and experimental LED phytoirradiator in clonal micropropagation of garden strawberry (Fragaria × ananassa, Duchesne ex Weston)

2019 ◽  
Vol 20 (4) ◽  
pp. 324-333 ◽  
Author(s):  
M. G. Markova ◽  
E. N. Somova
Keyword(s):  
Experimental Data ◽  
Growth Regulators ◽  
Growth Regulator ◽  
Fragaria Ananassa ◽  
Clonal Micropropagation ◽  
Fluorescent Lamps ◽  
Combined Use ◽  
The Impact ◽  
Reproduction Factor

The article provides experimental data of 2017-2018 study on the effect of growth regulators and LED phytoirradiator on the proliferation and rooting of promising garden strawberry (Fragaria ananassa) varieties in vitro. Micro-shoots of Korona and Brighton strawberry varieties were taken as the object of the research. Strawberry micro-shoots were cultivated under fluorescent lamps in the control variant. A programmable combined blinking LED phytoirradiator was under study. The combined effect of cytokinin and gibberellic acid by adding them to the Murashige and Skoog nutrient medium, as well as the impact of Siliplant and EcoFus growth regulators on strawberry micropropagation has been studied. It was established that in the cultivation of Korona variety the combined use of Siliplant and EcoFus under illumination with LED phytoirradiator provided an increase in the reproduction factor. The coefficient was 5.0 pcs./explant that was 1.7 times higher than the control (3.0 pcs/explant), the LSD05 1.4 pcs/explant. The maximum reproduction factor of remontant strawberry Brighton variety was obtained in the variant with the use of Siliplant and LED phytoirradiator and amounted to 4.9 pcs./explant (4.2 pcs./explant in the control), the LSD05 was 1.5 pcs./ explant. Regardless of the lighting, the use of RibavExtra in all variants under study increased the rooting rate of the strawberry Korona micro-shoots from 92.8 to 99.1%, the LSD05 6.1%. The use of LED phytoirradiator in comparison with the luminescent one (94.3%) provided a significant increase in the rooting rate of the strawberry Korona micro-shoots to 98.1% regardless of the growth regulators used, the LSD05 3.5%. The combined use of LED phytoirradiator and Ribav-Extra growth regulator in concentrations of 1.0 and 1.5 mg/l resulted in rooting of strawberry Korona micro-shoots up to 100%. Regardless of the growth regulator used, the use of LED phytoirradiator in comparison with the luminescent one (88.9%) provided a significant increase in the rooting rate of the strawberry Brighton micro-shoots to 97.2%, the LSD05 4.6%. The rooting rate of the remontant strawberry Brighton microshoots was 100% in the variant with the use of Ribav-Extra in the concentration of 1.0 mg /l combined with LED phytoirradiator 20 days after transplanting for rooting.

scholarly journals Introduction to in vitro culture of Ginkgo biloba (Linnaeus, 1771)

2020 ◽  
Vol 203 (12) ◽  
pp. 43-49
Author(s):  
Varvara Bessonova ◽  
Ol'ga Cherepanova
Keyword(s):  
Ginkgo Biloba ◽  
Optimal Growth ◽  
Growth Conditions ◽  
Biologically Active ◽  
Future Research ◽  
Planting Material ◽  
Murashige Skoog ◽  
Young Leaves ◽  
High Proliferative Activity

Abstract. The purpose of this research was to introduce Ginkgo biloba into culture, to study the composition and properties of its biologically active compounds. Methods. We researched the optimal growth conditions for obtaining a viable tissue culture, such as: concentration of phytohormones and other organic and nonorganic substances in Murashige – Skoog medium and light hours. The effectiveness of the standard method of sodium hypochloride sterilization of young leaves and vegetative buds also was verified. As a result, of conducting the experiment we were able to grow a living callus from leaves of G. biloba. Based on this result we can conclude that these conditions are acceptable for high proliferative activity of the plant. We were studied the effect of phytohormones NAA, at a concentration of 0.5 ml and 6-BAP, at a concentration of 2.5 ml. Also, was selected the ideal planting material for callus production – young leaves that were more sensitive to treatment with hypochloride. This research serves as the foundation for future research not only for our laboratory, but also for other research groups. The callus can be used to clone specimens of G. bilobain greenhouses. It will be use to extract and study unique chemical compounds, such as ginkgolides, bilobalides and various terpenes, contained in the extract of plants of this group.

USING OF LARGE WAX MOTH LARVAE PRODUCTS EXCRETED IN CLONAL MICROREPRODUCTION OF GARDEN STRAWBERRY

1970 ◽  
pp. 66-68
Author(s):  
М. G. Markova ◽  
Е. N. Somova
Keyword(s):  
Aqueous Solution ◽  
Survival Rate ◽  
Nutrient Medium ◽  
Galleria Mellonella ◽  
Waste Product ◽  
Biologically Active ◽  
Clonal Micropropagation ◽  
Wax Moth

Work on the clonal micropropagation of strawberries comes down to the search for new growth regulators, which include a biologically active substance - the waste product of the wax moth Galleria mellonella L. The effect of the waste product of the wax moth on the efficiency of clonal micropropagation of strawberries (Fragaria х ananassa duch) in vitro and in vivo conditions in 2018-2020 is shown. The object of research is micro-cuttings, rooted micro-cuttings and adapted micro-plants of garden strawberries of the Korona variety and of the remontant strawberries of the Brighton variety. It was revealed that at the proliferation stage, the propagation coefficient of the Korona variety increased significantly with the introduction of the waste product of the wax moth in doses of 4.0 mg/L and 6.0 mg/L and amounted to 4.2 and 3.8 pcs./explant, respectively; for Brighton variety, the coefficient increased significantly when the dose of the waste product of the wax moth 2.0 mg/L and amounted to 4.6 pcs./explant. The introduction of the waste product of the wax moth in doses of 4.0 mg/L and 6.0 mg/L into the nutrient medium had a significant effect on the yield of Brighton micro-cuttings suitable for rooting: the yield was 95.5 and 94.1%, respectively 87.7% in the control. For the Korona variety, no significant positive effect of the waste product of the wax moth on this indicator was noted. The rooting of micro-cuttings of strawberries of both varieties significantly increased with the introduction of the waste product of the wax moth into the nutrient medium in all studied doses and amounted to 86.4-100% in the Korona variety, and 88.9-100% in the Brighton variety.  The survival rate of adaptable micro-cuttings of Corona variety strawberries when sprayed with an aqueous solution of the waste product of the wax moth at a dose of 4.0 mg/L was 100%; the maximum survival rate of micro-cuttings Brighton variety is 99.8% in the variant with spraying with an aqueous solution of the waste product of the wax moth at a dose of 6.0 mg/L.

Micropropagation of Highly Productive Forms of Diploid and Triploid Aspen

2014 ◽  
Vol 962-965 ◽  
pp. 681-690 ◽  
Author(s):  
Dmitriy Nikolaevich Zontikov ◽  
Svetlana Zontikova ◽  
Roman Sergeev ◽  
Alex Shurgin ◽  
M. Sirotina
Keyword(s):  
Growth Regulators ◽  
Source Material ◽  
Soil Conditions ◽  
Nutrient Media ◽  
Clonal Micropropagation ◽  
Planting Material ◽  
Triploid Clones

The stages of getting the planting material for laying plantations of the triploid aspen (Populustremula L.) are illustrated in this work. The source material selected from the genetic fund “The gigantic aspens” is characterized. The peculiarities of using the method of clonal micropropagation when getting the planting material, the usage of a rootstock as a source of donor explants for the introduction into the culture in vitro, the influence of a kind of nutrient media and growth regulators NAA and BAP on the growth and the development of microsprouts. The distinctions in the speed of the growth of the culture in vitro of diploid and triploid clones have been analyzed. The adaptation of the plants-regenerants to the soil-conditions.

Optimization of biotechnological process clonal micropropagation in vitro of Asparagus officinalis L.

2021 ◽  
Vol 12 (3) ◽  
Author(s):  
Y Kolomiiets ◽  
◽  
A Skuba ◽  
Keyword(s):  
Culture Medium ◽  
Asparagus Officinalis ◽  
Skoog Medium ◽  
Clonal Micropropagation ◽  
Planting Material ◽  
Regenerated Plants ◽  
Murashige And Skoog Medium ◽  
Macro And Micronutrients ◽  
Indole 3 Acetic Acid

The study presents the results of obtaining regenerated plants of asparagus from seeds. Surface sterilizing the seeds by 0,75% sodium hypochlorite for 30 min is effective, during this obtained 83% viable sterile plants. The Murashige and Skoog medium supplemented with 6‑benzylaminopurine (2 mg/L), inositol (100 mg/L) and thiamine (0,4 mg/L) was found to be the best for seed germination. The expediency of using kinetin (1 mg/L) as a growth regulator to obtain a homogeneous plant material was established. The reproduction coefficient was 6,0. Only 11% of the explants formed callus. For the selection needs and production of somaclonal variants, the use of the culture medium with indole-3-acetic acid (0,2 mg/L) and 6‑benzylaminopurine (1 mg/L) is justified. In this condition reproduction coefficient was 3,7, and the level of different intensity callusogenesis was 59%. The rooting of obtained plants was performed in Murashige and Skoog medium supplemented with a half dose of macro- and micronutrients and growth regulators. Rooting frequency was up to 63%. The knowledge of hormonal requirements helps to promote isolated tissue and cells technologies of asparagus with purpose of rapid propagation and obtaining healthy, high-quality planting material.

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