scholarly journals Beta-hydroxy-beta-methylbutyrate (HMB) Supplementation to Mouse Dams in Gestation Does Not Affect Fetal Weight Variation of Offspring or Placental Development (P09-003-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Anna Clarke ◽  
Nathan Horn ◽  
Gerald Shurson ◽  
Christopher Faulk ◽  
Lee Johnston
Keyword(s):  
Dietary Treatment ◽  
Placental Weight ◽  
Fetal Weight ◽  
Placental Development ◽  
Funding Sources ◽  
Weight Variation ◽  
Pulse Dose ◽  
Pregnant Mice ◽  
Placental Efficiency ◽  
Dietary Treatments

Abstract Objectives The objective of this study was to determine if supplementing mouse dam diets with β-hydroxy-β-methylbutyrate (HMB) calcium salt throughout gestation would improve pup fetal weight uniformity and placental development. Methods Data were collected from 27 genetically identical mouse dams and their offspring. Dams were assigned to one of 4 dietary treatments: Control (CON; n = 7), Low HMB (LHMB; 3.5 mg/g diet; n = 6), High HMB (HHMB; 35 mg/g diet; n = 7), and low HMB pulse dose (PUL; 3.5 mg/g diet; n = 7) from days 6 to 10 of gestation. All dams were fed a swine lactation derived corn-soy diet with HMB supplementation only during gestation. Dams were euthanized on day 18 of gestation. Results Dietary treatment did not affect total number of pups per litter, but fetal weight was greater (P < 0.05) for pups from PUL dams (1.05 ± 0.02 g) than LHMB (0.94 ± 0.02 g) or HHMB (0.95 ± 0.02 g) dams. Within-litter variation (standard deviation and coefficient of variation) and range of fetal weights was not different among treatments. Differences between the median fetal weight within litter and weight of the lowest weight fetal pup in each litter were similar among treatments. Supplementation with HMB did not influence weight of placentae or area of the placental labyrinth. Placental efficiency, measured as fetal weight/placental weight, was less (P < 0.05) for LHMB dams compared to CON dams. Conclusions In conclusion, dietary supplementation of HMB for pregnant mice had no effect on fetal weight variation within litter. Supplementing diets with β-hydroxy-β-methylbutyrate had no effect on placental weight or labyrinth area but reduced placental efficiency in dams fed LHMB. Funding Sources This research has been supported and funded by BioMatrix International, Princeton, MN.

scholarly journals Paternal High Fat Diet and Exercise Differentially Regulate Placental Development and Inflammation in a Sex-Specific Manner in C57BL6/J Mice (P19-003-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Kate Larson ◽  
Amy Bundy ◽  
Travis Alvine ◽  
James Roemmich
Keyword(s):  
Skeletal Muscle ◽  
Agricultural Research ◽  
Wheel Running ◽  
Fetal Weight ◽  
High Fat ◽  
Placental Development ◽  
Funding Sources ◽  
Service Project ◽  
Diet And Exercise ◽  
Placental Inflammation

Abstract Objectives We have shown that increases in T2D risk in male offspring when the father consumes a high-fat (HF) diet can be normalized when the father also exercises during preconception, and that this protection may occur by epigenetic increases in insulin signaling within offspring skeletal muscle. In our current study, we investigated to determine how paternal HF diet and exercise conditions alter sperm miRNA, fetal weight and placental inflammation. Methods Three-week old male C57BL/6 mice were fed a normal-fat (NF) diet (16% fat) or a HF diet (45% fat) and assigned to either voluntary wheel running exercise or cage activity for 3 months prior to mating with NF diet fed dams. Sperm samples were collected to determine changes in miRNA that may account for the enhanced offspring skeletal muscle responses that helped normalize paternal HF-induced glucose intolerance. Placentae were collected to determine whether changes in sperm miRNA expression differed by amount of placental inflammation. Results Sperm expression of miRNA 193b increased with paternal HF and exercise. In F1 males, placental and fetal weight decreased with HF diet while, in F1 female, paternal HF and exercise had no effect on placental and fetal weights. Paternal HF diet decreased placental IL-6 and TNF-alpha mRNA expression in F1 females, while no effects were observed in F1 male placenta. Conclusions Taken together these data suggest that paternal HF diet has a greater impact on placental development of male fetuses while paternal exercise has greater impact on placental inflammation of female fetuses. For both female and male fetuses, these paternal influences are mediated via sperm miRNA 193b. miR-193b is involved in regulation of the cell cycle and adipogenesis but may have additional functions. Thus, the exact role of sperm miRNA 193b in sex-specific epigenetic transmission of paternal HF diet and exercise on placental and fetal development needs further evaluation. Funding Sources USDA Agricultural Research Service Project #3062-51000-052-00D.

126. IMPLICATIONS FOR FETAL AND PLACENTAL DEVELOPMENT FOLLOWING MITOCHONDRIAL PERTURBATION IN THE EMBRYO

2009 ◽  
Vol 21 (9) ◽  
pp. 45
Author(s):  
S. Wakefield ◽  
M. Lane ◽  
M. Mitchell
Keyword(s):  
Mitochondrial Function ◽  
Weight Ratio ◽  
Threshold Effect ◽  
Blastocyst Stage ◽  
Placental Weight ◽  
Placental Development ◽  
Cell Stage ◽  
Crown Rump Length ◽  
Fetal Survival ◽  
Placental Efficiency

The environment an embryo is exposed to can profoundly influence peri- and post-natal development despite having some capacity to adapt. Whilst the mechanisms responsible remain largely unknown, mitochondria are a likely target. In this study we deliberately perturbed mitochondrial function in the mouse embryo, using a model we have established that shows step-wise changes in embryo metabolism and development. The aim of this study was to provide direct evidence implicating mitochondrial dysfunction in the embryo with perturbed fetal and placental development. Zygote stage embryos were recovered from superovulated female mice and cultured in control conditions to the 2-cell stage. Embryos were then allocated to one of three treatments; control media (0μM-AOA), 5μM or 50μM of the known mitochondrial inhibitor, Amino-Oxyacetate, in the absence of pyruvate (5μM-AOA, 50μM-AOA). Embryos were cultured to the blastocyst stage, then transferred to pseudopregnant recipients, with fetal and placental parameters measured on day 18 of pregnancy. Implantation rates and fetal survival for both 5μM-AOA and 50μM-AOA was comparable to control embryos. For 5μM-AOA there was a significant reduction in placental weight (P=0.02) but normal fetal weight, and a significant increase in fetal: placental weight ratio (P=0.002) relative to the control, suggesting increased placental efficiency. When mitochondria were further perturbed (50μM-AOA), the fetuses and placentas were both considerably compromised: that is, decreased fetal and placental weights (P=0.002), reduced placental diameter (P=0.03) and decreased fetal crown rump length (P=0.07). This study demonstrates that mitochondrial function in the embryo impacts on peri-natal development, providing compelling evidence for mitochondrial function involvement in the mechanisms underpinning “embryo programming”. This data suggests a threshold effect, whereby embryos can only adapt up until a point after which development is compromised. Further elucidating these mechanisms is important for understanding how maternal environments and embryo culture systems determine development of future offspring.

scholarly journals Assisted reproduction causes placental maldevelopment and dysfunction linked to reduced fetal weight in mice

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Shuqiang Chen ◽  
Fang-zhen Sun ◽  
Xiuying Huang ◽  
Xiaohong Wang ◽  
Na Tang ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Metabolic Profile ◽  
Reproductive Technology ◽  
Fetal Weight ◽  
Imprinted Genes ◽  
Compelling Evidence ◽  
Placental Development ◽  
Placental Efficiency ◽  
Art Display ◽  
And Function

Abstract Compelling evidence indicates that stress in utero, as manifested by low birth weight (LBW), increases the risk of metabolic syndrome in adulthood. Singletons conceived by assisted reproductive technology (ART) display a significant increase in LBW risk and ART offspring have a different metabolic profile starting at birth. Here, used mouse as a model, we found that ART resulted in reduced fetal weight and placental overgrowth at embryonic day 18.5 (E18.5). The ART placentae exhibited histomorphological alterations with defects in placental layer segregation and glycogen cells migration at E18.5. Further, ART treatments resulted in downregulation of a majority of placental nutrient transporters and reduction in placental efficiency. Moreover, the ART placentae were associated with increased methylation levels at imprinting control regions of H19, KvDMR1 and disrupted expression of a majority of imprinted genes important for placental development and function at E18.5. Our results from the mouse model show the first piece of evidence that ART treatment could affect fetal growth by disrupting placental development and function, suggests that perturbation of genomic imprinting resulted from embryo manipulation may contribute to these problems.

35 Effect of Late Gestational Heat Stress on Placental Characteristics in Dairy Cattle

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 34-34
Author(s):  
Greer M Potadle ◽  
Geoff Dahl ◽  
Thaigo Fabris ◽  
Jennifer M Bundy ◽  
Howard Tyler
Keyword(s):  
Heat Stress ◽  
Surface Area ◽  
Pearson Correlation ◽  
Correlation Coefficients ◽  
Placental Weight ◽  
Late Gestation ◽  
Placental Development ◽  
Surface Areas ◽  
Humidity Index ◽  
Placental Efficiency

Abstract To determine the effects of late gestation heat stress on placental development, dairy cows were exposed to heat stress (HT, shade) or cooled (CL, shade, fans and soakers) during the final 46 d pre-calving on the University of Florida dairy facility (temperature-humidity index; THI &gt;68). We hypothesize heat stress (or lack of heat abatement) will reduce placental efficiency and in turn increase placental weight, surface area, and volume. At expulsion all placentae were collected and total placental weight was determined as well as individual cotyledonary weights, surface areas, and volume. In addition, the total number of total cotyledons was recorded and cotyledonary color and placental growth abnormalities (i.e. teratomas) were recorded and photographed. All data were analyzed using PROC MIXED in SAS. In addition, associations between parameters were determined by calculating Pearson Correlation Coefficients using SAS. Placentae from HT cows had a higher total placental weight, higher non-vascular membrane weight, higher total cotyledonary weight, higher total cotyledonary volume, and a higher incidence of teratomas than those from CL cows (P &lt; 0.05). HT cows also had placentae with a significantly greater average cotyledonary weight and volume (P &lt; 0.05). HT cows tended to have a greater incidence of color abnormalities in the placenta (P &lt; 0.075). In addition, HT cows had significantly lighter calves at birth (P &lt; 0.05). These data demonstrate that heat stress (or lack of heat abatement) impacts placental growth during the final stages of gestation, resulting in heavier placentae, an increase in cotyledonary weight and volume, but not an increase in the total number of cotyledons or total cotyledonary surface area. These placental alterations ultimately resulted in lighter calves at birth.

Influences on fetal and placental weights during mid to late gestation in prolific ewes well nourished throughout pregnancy

2000 ◽  
Vol 12 (4) ◽  
pp. 149 ◽  
Author(s):  
Paul L. Greenwood ◽  
Ramona M. Slepetis ◽  
Alan W. Bell
Keyword(s):  
Litter Size ◽  
Growth Retardation ◽  
Intrauterine Growth Retardation ◽  
Placental Weight ◽  
Late Gestation ◽  
Fetal Weight ◽  
Intrauterine Growth ◽  
Weight Variation ◽  
Condition Score ◽  
Tightly Coupled

This study investigated associations between fetal and placental weights from 85 to 130 days gestation in 49 fetuses from 21 ewes of a prolific genotype used as an experimental model of intrauterine growth retardation. The proportion of variation in fetal weight explained by placental weight increased from zero at 85 days to 91% (residual standard deviation (RSD) = 260 g) at 130 days. Overall, stage of pregnancy plus placental weight accounted for 96% of fetal weight variation (RSD = 212 g). Litter size and number of fetuses per uterine horn also influnced individual fetal weights. Gestational age, litter size, placental weight per ewe, and liveweight and condition score of ewes during early to mid gestation (initial LW and CS) explained 99.5% of the variation in fetal weight per ewe (RSD = 236 g). Most variation (86%) in placental weight was explained by stage of pregnancy, litter size, number of placentomes, and initial LW and CS (RSD = 53 g). Placental weight per ewe was influenced by stage of pregancy, litter size and initial ewe LW and CS (R 2 = 0.97; RSD = 89 g). The association of fetal and placental weights with initial ewe LW was positive, and with initial CS was negative. The results show that in the absence of overt nutritional restriction of pregnant ewes, fetal and placental weights are tightly coupled during late gestation and ewe fatness during early pregnancy is inversely related to placental and fetal weights. They demonstrate that placental weight explains most of the variation in fetal weight in the present intrauterine growth retardation model.

Physical and Chemical Characteristics of Meats of the Indonesian Indigenous Crossbred Chickens Fed Fermented Mixture of Papaya (Carica papaya L.) Leaf and Seed Meal

2020 ◽  
Keyword(s):  
Crude Protein ◽  
Control Diet ◽  
Dietary Treatment ◽  
Seed Meal ◽  
Chemical Characteristics ◽  
Breast Meat ◽  
Ph Values ◽  
Physical And Chemical Characteristics ◽  
Dietary Treatments ◽  
Physical And Chemical

The objective of this present study was to investigate the effect of feeding fermented mixture of papaya leaf and seed meal (FERM) on the physical and chemical characteristics of meats of the Indonesian indigenous crossbred chicken (IICC). The study was carried out with 300 day-old IICC. The chicks were randomly distributed to five treatment groups, i.e., CONT (control diet based on corn-soybean- diet), FERMA (diet containing 1% FERM), FERMB (2.5% FERM), FERMC (5% FERM) and FERMD (7.5% FERM). Each treatment group consisted of 6 replicates with 10 IICC in each. At week 8, one chick from each replicate was randomly taken and slaughtered. After being de-feathered and eviscerated, samples from breast and thigh meats were obtained. Results showed that the increased levels of FERM was followed by the increased (P<0.05) pH values, moisture and crude protein content of breast meats of the IICC. Dietary incorporation of FERM especially at the level of 2.5% increased (P<0.05) the content of fat in the breast meat of IICC, while further increased levels of FERM did not alter (P>0.05) the fat content of the IICC breast meat. Dietary treatment did not have any effect (P>0.05) on the lightness (L*) values of breast meat of the IICC. The redness (a*) values were higher (P<0.05) in FERMD breast meat than other. FERM diet resulted in lower (P<0.05) yellowness (b*) values in the IICC breast meat. The pH values and moisture content of thigh meat increased (P<0.05) with the increased level of FERM. The WHC decreased (P<0.05) with the elevated levels of FERM in the diets. There was an increase (P<0.05) in crude protein concentration in FERMA as compared to the other meats. Crude fat and ash concentrations in thigh meat were affected (P<0.05) by the treatments. Dietary treatments had no impact (P>0.05) on L* values of thigh meats. Feeding FERM at 7.5% from diets increased (P<0.05) and decreased (P<0.05) the redness and yellowness of meats. In conclusion, dietary inclusion of FERM especially at the level of 7.5% from diets improved the physical and chemical characteristics of the IICC meats.

scholarly journals PSVI-1 Dietary protease supplementation on growth performance and post-weaning diarrhea of nursery pigs

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 191-192
Author(s):  
Jorge Y Perez-Palencia ◽  
David A Clizer ◽  
Christian Ramirez-Camba ◽  
Ryan S Samuel ◽  
Crystal L Levesque
Keyword(s):  
Growth Performance ◽  
Phase 1 ◽  
Dietary Treatment ◽  
Experimental Period ◽  
Phase 2 ◽  
Deficient Diet ◽  
Nursery Pigs ◽  
Feeding Program ◽  
Dietary Treatments ◽  
Fecal Score

Abstract This experiment evaluated the effect of dietary protease supplementation on pig’s growth performance and post-weaning diarrhea in a 43-d trial. A total of 936 weaned pigs [21 d of age, initial BW 5.87 ± 0.31 kg] were used in a 2 × 2 factorial design (2 nutritional levels × 2 protease levels). The nutritional levels corresponded to a standard feeding program and a reduced feeding program (15% lower SID Lys) and without or with protease supplementation (0.0125% inclusion). Piglets were distributed in 36 pens with 26 pigs per pen and 9 replicates per dietary treatment. Pigs received a common Phase 1 diet and experimental diets in Phase 2, 3, and 4, (0.91, 3.4, 4.5, and 24.0 kg/pig, respectively). Phytase was included at 2,500 U in Phase 2 – 4, all diets met energy and AA:Lys requirements for nursery pigs. Feed intake and body weight were determined every 2 weeks. A pen fecal score assessment was performed at d7, 10, and 14. Performance data were analyzed using the PROC MIXED procedure and fecal score data using the PROC FREQ procedure (SAS Inst., Inc., Cary, NC). No significant interactions (P &gt; 0.05) were observed between dietary treatments. From d15 to 43 and during overall experimental period, pigs fed standard diets had greater BW, ADG, and G:F (P &lt; 0.05) than pigs fed deficient diets (Table 1). On d7, pigs fed deficient diet had more (X2 &lt; 0.05) soft and watery feces, and consequently less normal feces compared to pigs fed standard diets (Figure 1). On d7, 10, and 14, pigs fed diets with protease supplementation had more (X2 &lt; 0.05) normal feces compared to control pigs. Overall, effects of dietary protease supplementation on growth performance were not expressive under the conditions of this study but protease addition benefited intestinal health of nursery pigs.

scholarly journals 285 Effects of dietary MINTREX trace minerals and validation of serum biomarkers in measuring lameness in pigs

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 110-111
Author(s):  
Karen J Wedekind ◽  
Ashley Provin ◽  
Chelsie Foran ◽  
Tom Hampton ◽  
Ping Ren ◽  
...  
Keyword(s):  
Dietary Treatment ◽  
Cartilage Degradation ◽  
Serum Biomarkers ◽  
Trace Minerals ◽  
Average Score ◽  
Bone Degradation ◽  
Welfare Issue ◽  
Gait Score ◽  
Dietary Treatments ◽  
Main Effects

Abstract Lameness is a welfare issue and is the most common involuntary reason for culling pigs. Study objectives were to compare objective measures of lameness to gait score and assess effects of dietary chelated trace minerals in finisher pigs. The study was a 2 x 2 factorial arrangement consisting of naturally- occurring lame vs healthy pigs and 2 dietary treatments: 1) metal methionine hydroxy analogue chelate (MMHAC) supplied as MINTREX® Zn-Cu-Mn (Novus International, Inc.) supplemented at 80-10-20 mg/kg diet; and 2) sulfates Zn-Cu-Mn supplemented at 120-20-40 mg/kg diet. The study consisted of 4 groups of pigs (50-70 kg; 8 lame/8 non-lame per group) staggered one month apart. Dietary treatments were fed 53d. Measurements included gait scoring (0-4) and a panel of serum biomarkers (P2CP, CTX2, C2C, CTX1, osteocalcin) and were measured at baseline, d28 and d53. Serum biomarkers included bone synthesis (osteocalcin), bone degradation (CTXI), cartilage synthesis (P2CP), and cartilage degradation (C2C, CTX2) as well as the ratio of synthesis/ degradation for a total of 8 biomarkers. Proc Mixed GLM procedure of SAS was used and means were determined using a Tukey test. The main effects of healthy vs lame and dietary treatment were significant, but no diet x lameness interactions were observed. Lame pigs had elevated CTX2 (P&lt; .01), C2C (P=.02) and tended to have reduced ratio of P2CP:CTX2 (P=.09) compared to healthy pigs. MMHAC increased P2CP (P=.01) and P2CP:CTX2 ratio (P=.03) in both healthy and lame pigs compared to sulfates. These findings demonstrate that biomarkers can distinguish mildly lame pigs (average score of 1) from healthy pigs and lower concentrations of MMHAC vs commercial level of sulfates increased cartilage synthesis and the ratio of cartilage synthesis/degradation in both lame and healthy pigs.

Relationship between fetal weight and placenta

1927 ◽  
Vol 23 (2) ◽  
pp. 250-250
Keyword(s):  
Maternal Age ◽  
Full Term ◽  
Placental Weight ◽  
Fetal Weight ◽  
First Birth ◽  
Subsequent Birth ◽  
Mathematical Formulas

The relation of fetal and placental weight under different conditions was studied by Westermark on a material of 20,000 cases, using mathematical formulas, and he came to the following conclusions: fetal weight is less at first birth than at subsequent birth and is independent of the age of the mother; the placenta of full-term fetuses weighs on average 644.0 in boys and 657.0 in girls; the ratio of placental to fetal weight in boys is 1: 5.47, in girls 1:5.17; fetal weight arrives faster than placenta weight; the number determining the proportion between fetal weight and placenta weight increases with maternal age, but is independent of the number of former pregnancies; it is greater in twins.

scholarly journals Effect of replacing maize with cassava/brewers dried yeast blend (Cassayeast) on performance of broiler chicks and feed cost in southern Nigeria

2021 ◽  
Vol 30 (2) ◽  
pp. 169-178
Author(s):  
B. M. Oruwari ◽  
A. O. Anibo ◽  
D. M. Nkanta
Keyword(s):  
Control Diet ◽  
Dietary Treatment ◽  
Feed Utilization ◽  
Feed Ratio ◽  
Broiler Chicks ◽  
Feeding Trial ◽  
Total Replacement ◽  
Feed Cost ◽  
Dietary Treatments ◽  
The Cost

A feeding trial using 240, day old Anak broiler chicks was conducted in which cassava meal (CM) completely replaced maize in the control diet (A) to formulate five isocaloric and isonitrogenous diets. Dried CM blended with brewers dried yeast (BDY) formed cassayeast with 2.704 parts of CM to 1 part of BDY. The (Cassayeast) CM and BDY blend in the dietary treatments, (B,C,D and E) were 30 CM + 11.1% BDY, 35 CM +12.94% BDY, 40 CM + 14.79% BDY, and 45 CM + 16.64% BDY, respectively, and these treatments had no maize. Dietary treatment A, containing maize, which served as the control had no cassayeast inclusion, thus demonstrating the total replacement of maize by cassayeast in the other dietary treatments. Results showed that significant of maize difference (P<0.5) were observed in the control and at in one cassayeast treatment in feed intake (4.43 as against 4.22kg), body weight (1.94 as against 1.65kg), efficiency of feed utilization (feed: gain ratio 2.19 as against 2.51; gain: feed ratio 0.49 as against 0.43), respectively. However, gizzard weights were not significant. Economic analysis showed that the cost of producing a bird with the maize based diet (A) was significantly (P<0.05) higher than that with the cassayeast diets but there was not difference among the cassayeast diets (B-E). The maize based diet was most expensive (₦ 29.68 per kg diet) while diet C was the cheapest (₦ 25.57 per kg). The study demonstrated that the use of cassaveast has a place in the broiler industry, thus indicating that with proper protein balancing, cassava can completely replace maize in broiler diets.

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