Clinical acceptance of a multi-test reference region for biochemical-panel results.

1983 ◽  
Vol 29 (10) ◽  
pp. 1724-1726 ◽  
Author(s):  
T C Durbridge
Keyword(s):  
Signal Strength ◽  
Reference Intervals ◽  
Healthy People ◽  
Test Results ◽  
Reference Region ◽  
Trial Period ◽  
Biochemical Tests ◽  
Practical Advantage ◽  
Hospital Inpatients ◽  
Apparently Healthy

Abstract Results of a panel of six biochemical tests on a patient's specimen were mathematically combined into a "six-test signal strength" (STSS) value. This value indicated the overall extent of change from physiological results, and it was calculated in a way that ensured that a STSS value less than or equal to 1 occurred in 95% of apparently healthy people. STSS was reported with the test results for hospital inpatients during a four-month trial period. Doctors requested a repeat of the panel less often when a low STSS was reported, even if some test results were outside their separate reference intervals. Clinicians expressed differing opinions about its usefulness, some finding that a high STSS value had saved them from overlooking abnormal results, others not finding the value to be any practical advantage. Using a multi-test normal region resolves a statistical dilemma, while compounding the problem of knowing what results really mean.

scholarly journals EPIDEMIOLOGICAL INVESTIGATION ON DUCK SALMONELLOSIS IN SOME SELECTED AREAS OF BANGLADESH

2017 ◽  
Vol 14 (2) ◽  
pp. 149-160
Author(s):  
R. K. Dey ◽  
M. S. R. Khan ◽  
K. H. M. N. H. Nazir ◽  
M. A. Islam ◽  
S. M. S. H. Belal
Keyword(s):  
Clinical Signs ◽  
Epidemiological Data ◽  
P Value ◽  
Test Results ◽  
Biochemical Tests ◽  
Fecal Samples ◽  
Antibiotic Susceptibility Test ◽  
Highly Sensitive ◽  
Structured Questionnaire ◽  
Apparently Healthy

The study was performed with a view to collect epidemiological data to proper control of Duck Salmonellosis in Bangladesh. A total of 12 small to medium sized duck farms and 28 individual households were visited for data collection.  On the basis of history and clinical signs and as per the information provided by the farmers the prevalence rate of Duck Salmonellosis was recorded as 38.1% and the P value was calculated as 0.003 (p<0.01) which was noted as highly significant. Based on tentative occurrences of Duck Salmonellosis as per information taken from the structured questionnaire a total of 120 fecal samples were collected from apparently healthy and diseased ducks on the basis of age, sex, season, location and health status. The isolated bacteria were identified by studying cultural properties on different selective media, biochemical tests, and finally by PCR. The test results of cultural and biochemical exhibited the typical characteristics of bacteria. On the basis of their cultural and biochemical characteristics it was found that among 120 fecal samples 32 (26.67%) were found to be positive for Salmonella and the P value was recorded as 0.0019 (p<0.01) which was also considered as highly significant. In motility test Duck Salmonella were identified as motile and all tested Duck Salmonella showed indole and VP test negative with MR test positive. In PCR reaction, the organism was further confirmed as Salmonella species using the SAL-G primer. Results of antibiotic susceptibility test shows that the selected isolated Salmonella were highly sensitive to ciprofloxacin and azithromycin, intermediate sensitive to tobramycin and gentamicin and resistant to Oxacillin. 

scholarly journals MODEL OF HUMAN METABOLIC AGE

2021 ◽  
Vol 77 (3) ◽  
pp. 71-75
Author(s):  
Anatoliy Pisaruk ◽  
Valerii Shatilo ◽  
Ivanna Shchehlova ◽  
Svitlana Naskalova ◽  
Ludmila Mechova
Keyword(s):  
Metabolic Syndrome ◽  
Accelerated Aging ◽  
Absolute Error ◽  
Healthy People ◽  
Clinical Tests ◽  
Average Absolute Error ◽  
Biochemical Tests ◽  
Anthropometric Parameters ◽  
Metabolic Age ◽  
Apparently Healthy

With aging, regular changes develop in metabolism, first of all, these are changes in lipid and carbohydrate metabolism. With accelerated aging, metabolic disorders are more expressed, which leads to the development of metabolic syndrome. The purpose of the work was to develop a method for calculating metabolic age using available clinical tests and to assess the rate of metabolic aging in people with metabolic syndrome. Materials and methods. The study involved 283 apparently healthy people aged 20 to 80 years and 82 people with metabolic syndrome. Anthropometric parameters and biochemical tests were measured for all people included in the study. The formula for calculating metabolic age was obtained by the method of stepwise multiple regression.Results. The calculation of the metabolic age in healthy people according to the formula we obtained showed that the average absolute error is 6.01 years. In 20.5% of people with metabolic syndrome, metabolic age exceeds chronological age by more than 10 years. At the same time, in the group of healthy people, the share of such people was only 4.2%. Conclusions. The method we have developed for assessing the rate of metabolic aging has a sufficiently high accuracy and can be used to assess the risk of developing metabolic syndrome and other agerelated pathology.

scholarly journals Effect of 1 mg/kg oral prednisolone on biochemical analytes in ten dogs: a cross-over study

2021 ◽  
Author(s):  
Helena Pettersson ◽  
Carl Ekstrand ◽  
Anna Hillström ◽  
Inger Lilliehöök
Keyword(s):  
Clinical Relevance ◽  
Control Period ◽  
Iron Concentration ◽  
Oral Prednisolone ◽  
Reference Intervals ◽  
Specific Reference ◽  
Test Results ◽  
Prednisolone Treatment ◽  
Anti Inflammatory ◽  
And Control

AbstractPrednisolone is used for treatment of inflammatory, allergic, neoplastic, and immune-mediated diseases in dogs. As a glucocorticoid, prednisolone has biochemical effects, which may interfere with the interpretation of biochemistry test results. The aim of this study is to investigate the effects of prednisolone treatment in an anti-inflammatory dose on common biochemical analytes in dogs and to evaluate the clinical relevance of the changes. Ten beagle dogs, enrolled in a cross-over study, were treated with oral prednisolone (1 mg/kg 24 h) for 10 days. Blood samples were collected at day 0, 1, 3, 6, 9, 10, 12, 16, and 20. Data was analyzed using a general linear model with time and treatment as fixed factors. Pairwise comparisons were done between prednisolone and control period for each dog and sampling. Significant results were further evaluated for clinical relevance using laboratory-specific reference intervals and reference change values (RCVs), when available. Statistically significant changes were observed for ALP activity and iron concentration, which increased to levels exceeding the RCV, and several results were outside reference intervals. Phosphate and bile acids increased significantly, while amylase, lipase, and cholesterol decreased significantly, but with mean/median results remaining within reference intervals. Anti-inflammatory prednisolone treatment did not induce significant changes in ALT, GLDH, GGT, cPLI, glucose, or calcium. Treatment with an anti-inflammatory dose of prednisolone induced changes in several analytes. Only the increases in ALP and iron were of such magnitude that they are expected to affect the clinical interpretation of test results.

scholarly journals Abnormal Electrocardiograms in Apparently Healthy People

Circulation ◽  
1960 ◽  
Vol 21 (2) ◽  
pp. 204-213 ◽  
Author(s):  
F. A. L. MATHEWSON ◽  
G. S. VARNAM
Keyword(s):  
Healthy People ◽  
Apparently Healthy

Pediatric reference interval verification for endocrine and fertility hormone assays on the Abbott Alinity system

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Mary Kathryn Bohn ◽  
Siobhan Wilson ◽  
Alexandra Hall ◽  
Khosrow Adeli
Keyword(s):  
Clinical Decision Making ◽  
De Novo ◽  
Reference Interval ◽  
Clinical Decision ◽  
Reference Intervals ◽  
Healthy Children ◽  
Confidence Limits ◽  
Test Results ◽  
Serum Samples ◽  
Abbott Architect

Abstract Objectives The Canadian Laboratory Initiative on Pediatric Reference Intervals (CALIPER) has developed an extensive database of reference intervals (RIs) for several biomarkers on various analytical systems. In this study, pediatric RIs were verified for key immunoassays on the Abbott Alinity system based on the analysis of healthy children samples and comparison to comprehensive RIs previously established for Abbott ARCHITECT assays. Methods Analytical performance of Alinity immunoassays was first assessed. Subsequently, 100 serum samples from healthy children recruited with informed consent were analyzed for 16 Alinity immunoassays. The percentage of test results falling within published CALIPER ARCHITECT reference and confidence limits was determined. If ≥ 90% of test results fell within the confidence limits, they were considered verified based on CLSI guidelines. If <90% of test results fell within the confidence limits, additional samples were analyzed and new Alinity RIs were established. Results Of the 16 immunoassays assessed, 13 met the criteria for verification with test results from ≥ 90% of healthy serum samples falling within the published ARCHITECT confidence limits. New CALIPER RIs were established for free thyroxine and prolactin on the Alinity system. Estradiol required special considerations in early life. Conclusions Our data demonstrate excellent concordance between ARCHITECT and Alinity immunoassays, as well as the robustness of previously established CALIPER RIs for most immunoassays, eliminating the need for de novo RI studies for most parameters. Availability of pediatric RIs for immunoassays on the Alinity system will assist clinical laboratories using this new platform and contribute to improved clinical decision-making.

scholarly journals Reference intervals for hematology test parameters from apparently healthy individuals in southwest Ethiopia

2018 ◽  
Vol 6 ◽  
pp. 205031211880762 ◽  
Author(s):  
Lealem Gedefaw Bimerew ◽  
Tesfaye Demie ◽  
Kaleab Eskinder ◽  
Aklilu Getachew ◽  
Shiferaw Bekele ◽  
...  
Keyword(s):  
Platelet Count ◽  
Blood Cell ◽  
White Blood Cell ◽  
Red Blood Cell ◽  
Hematological Parameters ◽  
Age Groups ◽  
Reference Interval ◽  
Reference Intervals ◽  
Southwest Ethiopia ◽  
Apparently Healthy

Background: Clinical laboratory reference intervals are an important tool to identify abnormal laboratory test results. The generating of hematological parameters reference intervals for local population is very crucial to improve quality of health care, which otherwise may lead to unnecessary expenditure or denying care for the needy. There are no well-established reference intervals for hematological parameters in southwest Ethiopia. Objective: To generate hematological parameters reference intervals for apparently healthy individuals in southwest Ethiopia. Methods: A community-based cross-sectional study was conducted involving 883 individuals from March to May 2017. Four milliliter of blood sample was collected and transported to Jimma University Medical Center Laboratory for hematological analysis and screening tests. A hematological parameters were measured by Sysmex XS-500i hematology analyzer (Sysmex Corporation Kobe, Japan). The data were analyzed by SPSS version 20 statistical software. The non-parametric independent Kruskal–Wallis test and Wilcoxon rank-sum test (Mann–Whitney U test) were used to compare the parameters between age groups and genders. The 97.5 percentile and 2.5 percentile were the upper and lower reference limit for the population. Results: The reference interval of red blood cell, white blood cell, and platelet count in children were 4.99 × 1012/L (4.26–5.99 × 1012/L), 7.04 × 109/L (4.00–11.67 × 109/L), and 324.00 × 109/L (188.00–463.50 × 109/L), respectively. The reference interval of red blood cell, white blood cell, and platelet count in adults was 5.19 × 1012/L (4.08–6.33 × 1012/L), 6.35 × 109/L (3.28–11.22 × 109/L), and 282.00 × 109/L (172.50–415.25 × 109/L), respectively. The reference interval of red blood cell, white blood cell, and platelet count in geriatrics were 5.02 × 1012/L (4.21–5.87 × 1012/L), 6.21 × 109/L (3.33–10.03 × 109/L), and 265.50 × 109/L (165.53–418.80 × 109/L), respectively. Most of the hematological parameters showed significant differences across all age groups. Conclusion: Most of the hematological parameters in this study showed differences from similar studies done in the country. This study provided population-specific hematological reference interval for southwest Ethiopians. Reference intervals should also be established in the other regions of the country.

Reference intervals for calcium, phosphate, and alkaline phosphatase as derived on the basis of multichannel-analyzer profiles.

1986 ◽  
Vol 32 (1) ◽  
pp. 76-79 ◽  
Author(s):  
T J Sinton ◽  
D M Cowley ◽  
S J Bryant
Keyword(s):  
Alkaline Phosphatase ◽  
Calcium Phosphate ◽  
Older Patients ◽  
Reference Intervals ◽  
Plasma Calcium ◽  
Test Results ◽  
Abrupt Increase ◽  
Multichannel Analyzer ◽  
The Mean ◽  
The One

Abstract In an attempt to demonstrate a rapid and economical approach to deriving reference intervals, we analyzed data on plasma calcium and phosphate from a multichannel analyzer for more than 20 000 subjects and data on alkaline phosphatase from more than 10 000 subjects. Subjects were selected by the criterion, that their results for constituents other than the one of interest were within current reference intervals. Thus we have been able to include older patients who have diseases that accompany old age, but that do not affect test results. The mean concentrations of calcium and phosphate decreased with increasing age in both sexes, except for an abrupt increase for women about the time of reaching menopause. Similarly, the mean alkaline phosphatase activity increased with age in both sexes, reflecting a skewed frequency distribution. Here also, there was an abrupt increase in the modal value for women near menopause.

scholarly journals Personalized Reference Intervals in Laboratory Medicine: A New Model Based on Within-Subject Biological Variation

2020 ◽  
Author(s):  
Abdurrahman Coşkun ◽  
Sverre Sandberg ◽  
Ibrahim Unsal ◽  
Coskun Cavusoglu ◽  
Mustafa Serteser ◽  
...  
Keyword(s):  
Steady State ◽  
Personalized Medicine ◽  
Clinical Chemistry ◽  
Reference Interval ◽  
Reference Intervals ◽  
Biological Variation ◽  
Test Results ◽  
Steady State Condition ◽  
Laboratory Test Results ◽  
Measurement Results

Abstract Background The concept of personalized medicine has received widespread attention in the last decade. However, personalized medicine depends on correct diagnosis and monitoring of patients, for which personalized reference intervals for laboratory tests may be beneficial. In this study, we propose a simple model to generate personalized reference intervals based on historical, previously analyzed results, and data on analytical and within-subject biological variation. Methods A model using estimates of analytical and within-subject biological variation and previous test results was developed. We modeled the effect of adding an increasing number of measurement results on the estimation of the personal reference interval. We then used laboratory test results from 784 adult patients (&gt;18 years) considered to be in a steady-state condition to calculate personalized reference intervals for 27 commonly requested clinical chemistry and hematology measurands. Results Increasing the number of measurements had little impact on the total variation around the true homeostatic set point and using ≥3 previous measurement results delivered robust personalized reference intervals. The personalized reference intervals of the study participants were different from one another and, as expected, located within the common reference interval. However, in general they made up only a small proportion of the population-based reference interval. Conclusions Our study shows that, if using results from patients in steady state, only a few previous test results and reliable estimates of within-subject biological variation are required to calculate personalized reference intervals. This may be highly valuable for diagnosing patients as well as for follow-up and treatment.

scholarly journals Changes in biochemical tests in pregnancy and their clinical significance

2018 ◽  
Vol 11 (4) ◽  
pp. 160-170 ◽  
Author(s):  
Stephanie Teasdale ◽  
Adam Morton
Keyword(s):  
Normal Pregnancy ◽  
Reference Intervals ◽  
Pathological State ◽  
Physiological Changes ◽  
Biochemical Tests ◽  
Biochemical Assays ◽  
Common Causes ◽  
Laboratory Investigations ◽  
Physiological Changes In Pregnancy ◽  
In Pregnancy

Interpretation of laboratory investigations relies on reference intervals. Physiological changes in pregnancy may result in significant changes in normal values for many biochemical assays, and as such results may be misinterpreted as abnormal or mask a pathological state. The aims of this review are as follows: 1. To review the major physiological changes in biochemical tests in normal pregnancy. 2. To outline where these physiological changes are important in interpreting laboratory investigations in pregnancy. 3. To document the most common causes of abnormalities in biochemical tests in pregnancy, as well as important pregnancy-specific causes.

scholarly journals Plasma midregional proadrenomedullin (MR-proADM) concentrations and their biological determinants in a reference population

2018 ◽  
Vol 56 (7) ◽  
pp. 1161-1168 ◽  
Author(s):  
Magdalena Krintus ◽  
Marek Kozinski ◽  
Federica Braga ◽  
Jacek Kubica ◽  
Grazyna Sypniewska ◽  
...  
Keyword(s):  
Reference Group ◽  
Adult Population ◽  
Reference Population ◽  
Cross Sectional Study ◽  
Reference Intervals ◽  
Cross Sectional ◽  
Multiple Organs ◽  
Laboratory Test Results ◽  
Biological Determinants ◽  
Apparently Healthy

Abstract Background: Midregional proadrenomedullin (MR-proADM) is emerging as a prognostic biomarker for detecting the failure of multiple organs. Establishment of scientifically robust reference intervals facilitates interpretation of laboratory test results. The objectives of this study were (i) to establish reliable reference intervals for plasma MR-proADM using a commercially available automated fluoroimmunoassay in apparently healthy individuals, and (ii) to identify biological determinants of MR-proADM concentrations. Methods: A total of 506 questionnaire-identified apparently healthy adults were enrolled in a single-center, cross-sectional study. A final reference group (n=172) was selected after exclusion of obese individuals, those with increased values of laboratory biomarkers indicating asymptomatic myocardial injury or dysfunction, ongoing inflammation, diabetes, dyslipidemia and renal dysfunction and outliers. Results: The 2.5th and 97.5th percentile intervals for MR-proADM values in the reference group (90% confidence interval) were 0.21 (0.19–0.23) and 0.57 (0.55–0.59) nmol/L, respectively. Although older age, higher values of HbA1c, C-reactive protein, B-type natriuretic peptide and body mass index, together with a history of smoking and a decreased estimated glomerular filtration rate were significantly associated with increasing concentrations of MR-proADM in both univariate and multivariate analyses, magnitudes of these relationships were modest and did not substantially influence MR-proADM reference intervals. Sex-dependent difference in MR-proADM reference intervals was not detected [0.19 (0.16–0.22)–0.56 (0.54–0.60) nmol/L in females vs. 0.22 (0.20–0.25)–0.58 (0.57–0.63) nmol/L in males]. Conclusions: Our study successfully established robust reference intervals for MR-proADM concentrations in plasma. Considering the negligible influence of potential biological determinants on plasma MR-proADM, we recommend the adoption of single reference intervals for adult population as a whole.

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