scholarly journals 231 Impact of Brachyspira hyodysentariae on intestinal function and integrity

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 116-116
Author(s):  
Emma T Helm ◽  
Susanne J Lin ◽  
Nicholas Gabler ◽  
Eric R Burrough
Keyword(s):  
Ex Vivo ◽  
Potato Starch ◽  
Nutrient Digestibility ◽  
High Morbidity ◽  
Post Inoculation ◽  
Intestinal Function ◽  
Treatment Groups ◽  
Intestinal Integrity ◽  
Beet Pulp ◽  
The Impact

Abstract Swine dysentery (SD) induced by Brachyspira hyodysentariae (Bhyo) causes colitis and mucohemorrhagic diarrhea in grow-finish pigs, however little is known about the physiological changes that occur to the gastrointestinal tract during Bhyo infection. Thus, the objective of this study was to evaluate the impact of a Bhyo challenge on intestinal function and integrity of pigs fed two divergent diets. A total of 36 Bhyo negative gilts (24.3 ± 3.6 kg BW) were selected and assigned to one of three treatment groups (n=12 pigs/trt): 1) Bhyo negative, 20% DDGS diet (CON), 2) Bhyo challenged, 20% DDGS diet (DDGS), and 3) Bhyo challenged, 10% DDGS, 5% beet pulp and 5% resistant potato starch diet (RS). Pigs were fed diets 21 days prior to challenge and on days post inoculation (dpi) 0 and 1, pigs were inoculated with Bhyo or sham. Fecal samples were collected for ATTD and pigs were euthanized for colon collection within 72 hours of initial observation of clinical SD, or at the end of the study (dpi 10-16). Tissues were assessed for ex vivo measures of intestinal integrity and mitochondrial function. The challenge resulted in high morbidity, with 88% of DDGS and RS pigs developing clinical SD. Colon transepithelial resistance was increased in DDGS pigs compared with CON and RS pigs (P=0.005), and colon macromolecule permeability was reduced in both DDGS and RS pigs compared with CON pigs (P=0.006), likely due to mucoid discharge. Colonic mitochondrial oxygen consumption was not impacted by treatment (P >0.10). Further, ATTD of DM, OM, N, and GE were reduced in DDGS pigs compared with CON pigs (P< 0.001), whilst nutrient digestibility was not reduced in RS pigs. Taken together, these data show Bhyo does not appear to reduce ex vivo colonic integrity. Further, the RS diet may reduce severity of a Bhyo challenge.

scholarly journals Lawsonia intracellularis infected enterocytes lack sucrase-isomaltase which contributes to reduced pig digestive capacity

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Emma T. Helm ◽  
Eric R. Burrough ◽  
Fernando L. Leite ◽  
Nicholas K. Gabler
Keyword(s):  
Dry Matter ◽  
Post Inoculation ◽  
Intestinal Function ◽  
Lawsonia Intracellularis ◽  
Sample Collection ◽  
Treatment Groups ◽  
Swine Herds ◽  
The Impact ◽  
Lesion Severity

AbstractLawsonia intracellularis is endemic to swine herds worldwide, however much is still unknown regarding its impact on intestinal function. Thus, this study aimed to characterize the impact of L. intracellularis on digestive function, and how vaccination mitigates these impacts. Thirty-six L. intracellularis negative barrows were assigned to treatment groups (n  =  12/trt): (1) nonvaccinated, L. intracellularis negative (NC); (2) nonvaccinated, L intracellularis challenged (PC); and (3) L. intracellularis challenged, vaccinated (Enterisol® Ileitis, Boehringer Ingelheim) 7 weeks pre-challenge (VAC). On days post-inoculation (dpi) 0 PC and VAC pigs were inoculated with L. intracellularis. From dpi 19–21 fecal samples were collected for apparent total tract digestibility (ATTD) and at dpi 21, pigs were euthanized for sample collection. Post-inoculation, ADG was reduced in PC pigs compared with NC (41%, P  <  0.001) and VAC (25%, P  <  0.001) pigs. Ileal gross lesion severity was greater in PC pigs compared with NC (P  =  0.003) and VAC (P  =  0.018) pigs. Dry matter, organic matter, nitrogen, and energy ATTD were reduced in PC pigs compared with NC pigs (P  ≤  0.001 for all). RNAscope in situ hybridization revealed abolition of sucrase-isomaltase transcript in the ileum of PC pigs compared with NC and VAC pigs (P  <  0.01). Conversely, abundance of stem cell signaling markers Wnt3, Hes1, and p27Kip1 were increased in PC pigs compared with NC pigs (P  ≤  0.085). Taken together, these data demonstrate that reduced digestibility during L. intracellularis challenge is partially driven by abolition of digestive machinery in lesioned tissue. Further, vaccination mitigated several of these effects, likely from lower bacterial burden and reduced disease severity.

scholarly journals PSIX-1 Altering dietary fiber components mitigates severity of Brachyspira hyodysentariae challenge

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 190-191
Author(s):  
Emma T Helm ◽  
Nicholas Gabler ◽  
Eric R Burrough
Keyword(s):  
Dietary Fiber ◽  
Potato Starch ◽  
Dietary Treatment ◽  
Mitigation Strategies ◽  
Post Inoculation ◽  
Insoluble Dietary Fiber ◽  
Beet Pulp ◽  
Pig Performance ◽  
Swine Herds ◽  
And Control

Abstract Swine dysentery (SD) induced by Brachyspira hyodysentariae (Bhyo) has recently become more prevalent in swine herds, renewing research interest regarding dietary mitigation strategies. It has been reported that insoluble dietary fiber such as DDGS influences Bhyo colonization leading to more rapid disease development. Therefore, the objective of this study was to determine if replacement of insoluble (20% DDGS) with soluble and highly fermentable [sugar beet pulp (BP) and resistant potato starch (RS)] fiber would reduce Bhyo disease expression. At total of 38 pigs (40.9 ± 5.0 kg BW) were selected, confirmed negative for Bhyo, and allocated to dietary treatment groups (13 pigs/trt): 1) Control consisting of 20% DDGS, no BP or RS (0%), 2) 10% DDGS, 5% BP and 5% RS (5%), or 3) 0% DDGS, 10% BP, 10% RS (10%). All diets were formulated to be isocaloric and isonitrogenous. Diets were fed for 14 days pre-challenge and on days post inoculation (dpi) 0, all pigs were inoculated with Bhyo. Pigs and feeders were weighed weekly for 28 dpi. Overall, ADG was greater in both 5% (0.85 kg/d) and 10% (1.18 kg/d) pigs compared with 0% pigs (0.63 kg/d; P=0.004). The 10% pigs (2.46 kg/d) had greater ADFI compared with the 0% pigs (1.84 kg/d; P=0.024), 5% pigs being intermediate (2.20 kg/d). The 10% pigs also had greater G:F compared with both the 0% and 5% pigs (P&lt; 0.001). In terms of clinical disease presentation, 11/13 0% pigs developed clinical SD compared with 6/13 5% pigs and only 2/13 10% pigs (P=0.002). In conclusion, while not completely protective, reducing insoluble dietary fiber via replacement with soluble and fermentable BP and RS reduced clinical SD and improved pig performance during a 28-day Bhyo challenge. These data suggest such dietary manipulation may reduce usage of antibiotics in SD treatment and control.

Impact of four fiber-rich supplements on nutrient digestibility, colostrum production, and farrowing performance in sows

2021 ◽  
Vol 99 (9) ◽  
Author(s):  
Takele Feyera ◽  
Liang Hu ◽  
Maria Eskildsen ◽  
Thomas S Bruun ◽  
Peter K Theil
Keyword(s):  
Dry Matter ◽  
Palm Kernel ◽  
Nutrient Digestibility ◽  
Extra Energy ◽  
Lower Blood ◽  
Nonstarch Polysaccharides ◽  
Gross Energy ◽  
Soy Hulls ◽  
Beet Pulp ◽  
The Impact

Abstract This study aimed to investigate the impact of dietary fiber (DF) sources on sow and litter performance, and apparent total tract digestibility (ATTD) of gross energy (GE) and nutrients. A total of 48 sows were stratified for body weight at mating and randomly assigned to one of four DF sources (mixed fiber [MF], palm kernel expellers [PKE], sugar beet pulp [SBP], or soy hulls [SH]) and fed the diet from mating until farrowing. Within DF treatments, sows were supplemented with one of two extra energy sources (glycerol or sugar dissolved in water), whereas a third group (control) received water from day 108 of gestation until farrowing. The number of total born, live-born, and stillborn pigs; birth time and birth weight of the pigs; farrowing duration; and farrowing assistance (FA) were recorded. Live-born pigs were weighed again at 12 and 24 h after birth to record weight gain, which was used to estimate intake and yield of colostrum. Blood samples were collected once daily from day −3 relative to farrowing until day 1 after farrowing in sows and once from selected pigs right after birth. Fecal samples were collected on day 114 of gestation and colostrum at 0, 12, 24, and 36 h after onset of farrowing. Intake of soluble and insoluble nonstarch polysaccharides (NSP) was greater for SBP (P &lt; 0.001) and PKE (P &lt; 0.001) supplemented sows, respectively, when compared with other groups. Farrowing duration and stillbirth rate were not affected by DF sources, but PKE and SH supplemented sows had greater FA than SBP and MF supplemented sows (P &lt; 0.001). Extra energy supplement did not improve the farrowing performance. Concentration (P = 0.02) and output (P = 0.04) of dry matter in colostrum, and ATTD of GE (P &lt; 0.001) and crude protein (CP; P &lt; 0.001) were lower for PKE supplemented sows than in sows from the remaining groups. Intake of insoluble NSP correlated negatively with ATTD of GE (P &lt; 0.001) and CP (P &lt; 0.001). Concentrations of glucose (P &lt; 0.001), lactate (P &lt; 0.001), CO2 (P &lt; 0.001), and HCO3 (P &lt; 0.001) in sows blood were increased with time progress relative to farrowing. Newborn pigs from PKE supplemented sows had greater concentration of lactate (P = 0.02) and lower blood pH (P = 0.02) than the remaining treatments. In conclusion, PKE supplement reduced ATTD of GE and CP, and concentration and output of dry matter in colostrum but increased FA. Results of this experiment indicated that the use of PKE as a fiber source for late gestating sows should be avoided.

The impact of heat stress on intestinal function and productivity in grow-finish pigs

2015 ◽  
Vol 55 (12) ◽  
pp. 1403 ◽  
Author(s):  
N. K. Gabler ◽  
S. C. Pearce
Keyword(s):  
Heat Stress ◽  
Gastrointestinal Tract ◽  
Well Being ◽  
Growing Pigs ◽  
Intestinal Function ◽  
Transport Pathways ◽  
Altered Expression ◽  
Intestinal Integrity ◽  
Increased Risk ◽  
The Impact

Heat stress is a physiological condition when animals can no longer regulate their internal euthermic temperature. When livestock such as pigs are subjected to this environmental stress, it can be detrimental to performance, health and well-being, and if severe enough even death. Growing pigs are particularly susceptible to heat stress and one of the major organs first affected by heat stress is the gastrointestinal tract. As a result, reductions in appetite, intestinal function and integrity and increased risk of endotoxemia can modify post-absorptive metabolism and tissue accretion. These changes in intestinal integrity may be a result of altered expression of tight junction proteins, increased circulating endotoxin concentrations and markers of cellular stress (heat shock and hypoxia response), which is evident as early on as 2 h after heat-stress onset. Due to restricted blood flow, the ileum is more severely affected compared with the colon. Interestingly, many of the negative effects of heat stress on intestinal integrity appear to be similar to those observed with pigs reared under reduced nutrient and caloric intakes. Altogether, these depress pig performance and health, and extend days to market. Despite this impact on the gastrointestinal tract, under heat-stress conditions, intestinal glucose transport pathways are upregulated. This review discussed how heat stress (directly and indirectly via reduced feed intake) affects intestinal integrity and how heat stress contributes to decreased growth performance in growing pigs.

scholarly journals Highly Fermentable Fiber Alters Fecal Microbiota and Mitigates Swine Dysentery Induced by Brachyspira hyodysenteriae

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 396
Author(s):  
Emma T. Helm ◽  
Nicholas K. Gabler ◽  
Eric. R. Burrough
Keyword(s):  
Average Daily Gain ◽  
Dietary Treatment ◽  
Fecal Microbiota ◽  
Post Inoculation ◽  
Swine Dysentery ◽  
Brachyspira Hyodysenteriae ◽  
Treatment Groups ◽  
Beet Pulp ◽  
Improved Performance ◽  
Corn Distillers

Brachyspira hyodysenteriae is an etiological agent of swine dysentery (SD). Diet fermentability plays a role in development of SD, but the mechanism(s) of action are largely unknown. Thus, this study aimed to determine whether replacing lowly fermentable fiber with highly fermentable fiber would mitigate a 42 d B. hyodysenteriae challenge. Thirty-nine barrows were allocated to dietary treatment groups: (1) 20% corn distillers dried grain with solubles (DDGS), 0% beet pulp (BP) or resistant starch (RS; lowly fermentable fiber (LFF)); (2) 10% DDGS, 5% BP, 5% RS (medium fermentable fiber (MFF)); and (3) 0% DDGS, 10% BP, 10% RS (highly fermentable fiber (HFF)). On day post inoculation 0, pigs were inoculated with B. hyodysenteriae. Overall, 85% LFF pigs developed clinical SD, 46% of MFF pigs developed SD, and 15% of HFF pigs developed SD (p < 0.05). Overall average daily gain (ADG) differed among all treatments (p < 0.001), with LFF pigs having the lowest ADG. For HFF pigs, ADG was 37% greater than LFF pigs (p < 0.001) and 19% greater than MFF pigs (p = 0.037). The LFF diet had greater relative abundance of Shuttleworthia and Ruminococcus torques. Further, microbiota of pigs that developed SD had enriched Prevotellaceae. Collectively, replacing DDGS with highly fermentable fiber reduced clinical SD, improved performance, and modulated fecal microbiota during B. hyodysenteriae challenge.

scholarly journals PSIV-18 Effect of sub-therapeutic antibiotics and pharmacological zinc oxide on nursery pig performance and intestinal integrity and function

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 190-190
Author(s):  
Carson De Mille ◽  
Emma T Helm ◽  
Crystal L Loving ◽  
Eric R Burrough ◽  
Nicholas K Gabler
Keyword(s):  
Zinc Oxide ◽  
T Cell ◽  
Ex Vivo ◽  
Ussing Chambers ◽  
Cell Abundance ◽  
Intestinal Integrity ◽  
Nursery Pig ◽  
Pig Performance ◽  
And Function ◽  
The Impact

Abstract The objective of this study was to evaluate the impact of sub-therapeutic chlortetracycline (CTC) and pharmacological zinc oxide on nursery pig performance and intestinal integrity and function. Twenty-four newly weaned pigs (4.51 ± 0.21 kg BW) were randomly assigned to one of three dietary treatments for 21 d (n = 8/treatment): 1) Control diet (NC), 2) NC + Zinc oxide at 3000 ppm for 7 d followed by 2000 ppm for 14 d (ZnO), and 3) NC + 40 ppm CTC for 21 d (sCTC). Pig ADG, ADFI, and G:F were determined for the overall 21 d test period. At d 21, all pigs were euthanized for tissue collection. Jejunum and ileum ex vivo transepithelial resistance (TER), macromolecule (FD4) flux, and active glucose and glutamine transport were assessed in modified Ussing Chambers. Additionally, jejunum brush border enzyme and Na+/K+ ATPase activities were determined. Morphology and T-cell abundance were determined in all ileum sections by H&E staining and immunohistochemistry, respectively. Compared to the NC, sCTC and ZnO increased ADG (0.14, 0.20 and 0.23 kg/d, respectively, P = 0.020). Overall ADFI was increased by 150% due to sCTC compared to the NC and ZnO pigs (P < 0.05). G:F was increased due to ZnO and sCTC compared to the NC (0.82, 0.63 and 0.47, respectively, P < 0.01). Jejunum and ileum ex vivo function and integrity did not differ. Ileal villus height was increased in the NC compared to sCTC treatment (P < 0.01), but not different from the ZnO treatment. In the ileum, total T-cell number per unit area was significantly lower in the ZnO segments compared to sCTC and NC (0.95, 2.29 and 2.59 count per μm2, respectively, P = 0.05). These data indicate that ZnO and sCTC improved pig performance but did not directly alter small intestinal integrity and function. However, mucosal T cell abundance was altered.

scholarly journals 364 Impact of porcine reproductive and respiratory syndrome virus challenge and deoxynivalenol on intestinal integrity and nutrient transport

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 148-149
Author(s):  
Samuel Gerrard ◽  
Emma T Helm ◽  
Blaire Todd ◽  
Nicholas K Gabler
Keyword(s):  
Glucose Transport ◽  
Fixed Effects ◽  
Ex Vivo ◽  
Respiratory Syndrome Virus ◽  
Ussing Chambers ◽  
Prrs Virus ◽  
Intestinal Integrity ◽  
Glutamine Transport ◽  
And Function ◽  
The Impact

Abstract Porcine reproductive and respiratory syndrome (PRRS) virus infections and mycotoxin contaminated feedstuffs are two common issues facing the pork industry as both act, independently, to antagonize pig intestinal function, growth performance, and health. However, the combined impact of PRRS and mycotoxin exposure on pig intestinal health and function has not been investigated. Therefore, the objective of this experiment was to examine the impact of the mycotoxin deoxynivalenol (DON) on the intestinal integrity and function of PRRS infected pigs, employing an ex vivo model. Jejunum samples were collected from PRRS naïve (control; n = 6) and 21 day post inoculated PRRS virus infected (n = 8) gilts (10 weeks of age). In duplicate, explants were mounted into modified Ussing Chambers and incubated with or without 25 mM DON for 45 minutes prior to assessment of transepithelial resistance (TER), mucosal to serosal macromolecule (FD4) flux, and active glucose and glutamine transport. Data were analyzed using the mixed model procedure of SAS and a split plot design to examine the fixed effects of PRRS, DON, and their interaction. There were no PRRS by DON interactions for any ex vivo parameters assessed. Irrespective of DON, PRRS jejunum segments had decreased TERs (57%, P < 0.001), increased FD4 flux (P = 0.005), and tended to have increased active glucose transport (P = 0.093) compared with uninfected controls. Irrespective of PRRS status, DON increased FD4 flux (113%, P = 0.013), and tended to increase active glucose transport (P = 0.088) compared with jejunal explants not treated with DON. However, TERs and active glutamine transport did not differ due to DON treatment. Taken together, these data highlight the impacts that viral health challenge and mycotoxin contamination have on aspects of intestinal integrity and function. However, it does not appear that PRRS challenged pigs are more sensitive to the effects of DON than their healthy counterparts.

scholarly journals PSV-8 Glucocorticoid receptor agonist (GRA): what is the best delivery method to newly weaned pigs?

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 158-159
Author(s):  
Hailey Wooten ◽  
Hwanhee Kim ◽  
Amanda Rakhshandeh ◽  
Anoosh Rakhshandeh
Keyword(s):  
Growth Performance ◽  
Performance Outcomes ◽  
Nutrient Digestibility ◽  
Control Group ◽  
Free Access ◽  
Weaned Pigs ◽  
Delivery Methods ◽  
Nursery Pigs ◽  
Intestinal Integrity ◽  
Randomized Design

Abstract We previously found that i.m. injection of GRA, similar to in-feed antibiotics, improves measures of intestinal integrity, nutrient digestibility, and overall growth performance in nursery pigs. In addition, we have demonstrated that the mitigating effects of GRA on the stress of weaning is predominantly done by subsiding the inflammatory response that often accompanies early weaning. The objective of the current study was to explore the most practical methods for delivering GRA to newly weaned pigs. One-hundred and sixty-seven PIC pigs were weaned at 25 ±1.0 days of age (BW 7 ±1.2 kg), and assigned to fourteen treatments (3-4 pigs /pen; 3 pens/treatment). Seven treatments were tested within two sexes (gilts vs. barrows). The treatments were: 1) i.m. injection of GRA (two injections, 0.2 mg/kg BW at -1 and 3 d post-weaning), 2) two levels of in-water GRA (0.8 and 1.6 ppm), 3) in-feed GRA (2.5 and 5.0 ppm), 4) in-feed antibiotic (ANT, 100 ppm tylosin), and 5) control. Dexamethasone was used as GRA. Pigs had free access to in-feed or in-water GRA or ANT during the 1st-week post-weaning. Parameters of growth performance were measured weekly until the end of the nursery phase. A completely randomized design and repeated measurement ANOVA (PROC MIXED) in SAS were used for statistical analyses. In-water, in-feed, and i.m. GRA treatments resulted in growth performance outcomes comparable to that of in-feed ANT. Relative to CON pigs, both ANT and GRA improved ADG and G: F during the nursery phase (P &lt; 0.05). Among the delivery methods tested, 2.5 ppm of in-feed GRA resulted in superior ADG (GRA vs. CON; 0.29 vs. 0.20 kg/d) and G: F (0.63 vs. 0.40 ± 0.05), compared to the control group (P &lt; 0.02). Collectively, these results suggested that in-feed treatment is the best method for delivering GRA to newly weaned pigs.

scholarly journals Histological, Biomechanical, and Biological Properties of Genipin-Crosslinked Decellularized Peripheral Nerves

2021 ◽  
Vol 22 (2) ◽  
pp. 674
Author(s):  
Óscar Darío García-García ◽  
Marwa El Soury ◽  
David González-Quevedo ◽  
David Sánchez-Porras ◽  
Jesús Chato-Astrain ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Nerve Repair ◽  
Wistar Rat ◽  
Biomechanical Properties ◽  
Biological Properties ◽  
Suitable Alternative ◽  
Promising Alternative ◽  
Histological Pattern ◽  
The Impact

Acellular nerve allografts (ANGs) represent a promising alternative in nerve repair. Our aim is to improve the structural and biomechanical properties of biocompatible Sondell (SD) and Roosens (RS) based ANGs using genipin (GP) as a crosslinker agent ex vivo. The impact of two concentrations of GP (0.10% and 0.25%) on Wistar rat sciatic nerve-derived ANGs was assessed at the histological, biomechanical, and biocompatibility levels. Histology confirmed the differences between SD and RS procedures, but not remarkable changes were induced by GP, which helped to preserve the nerve histological pattern. Tensile test revealed that GP enhanced the biomechanical properties of SD and RS ANGs, being the crosslinked RS ANGs more comparable to the native nerves used as control. The evaluation of the ANGs biocompatibility conducted with adipose-derived mesenchymal stem cells cultured within the ANGs confirmed a high degree of biocompatibility in all ANGs, especially in RS and RS-GP 0.10% ANGs. Finally, this study demonstrates that the use of GP could be an efficient alternative to improve the biomechanical properties of ANGs with a slight impact on the biocompatibility and histological pattern. For these reasons, we hypothesize that our novel crosslinked ANGs could be a suitable alternative for future in vivo preclinical studies.

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