scholarly journals PSVI-5 Effects of exogenous glucoamylase enzymes or a combination with a neutral protease on total tract apparent digestibility and feces D-lactate in bulls fed a total mixed ration rich in rolled corn

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 436-436
Author(s):  
Maria Devant ◽  
Shukun Yu ◽  
Sandra Genis ◽  
Torben Larsen ◽  
Wenting Li
Keyword(s):  
Aspergillus Fumigatus ◽  
Trichoderma Reesei ◽  
Bacillus Amyloliquefaciens ◽  
Lactate Concentration ◽  
Latin Square ◽  
Neutral Protease ◽  
Potassium Sorbate ◽  
Apparent Digestibility ◽  
Total Mixed Ration ◽  
Enzyme Preparations

Abstract The aim of this study was to evaluate the effect of 2 glucoamylases (GA) and the combination of one GA with a neutral protease on total tract apparent digestibility in bulls fed a total mixed ration (TMR) rich in rolled corn. Sixteen Angus beef bulls (266 ± 4.9 kg of initial BW, and 182 ± 1.7 d of age) were housed individually in pens and were distributed in 4 blocks of 4 animals balanced by BW. The experimental design was a 4 x 4 Latin square (4 blocks and 4 periods) with periods of 2 wks. Four treatments were tested; 1) control, a blank solution of 0.2% potassium sorbate and 0.6% sodium benzoate corresponding to the preservatives used in the enzyme preparation, 2) a GA preparation from Trichoderma reesei; 3) a GA preparation from Aspergillus fumigatus; 4) and a GA from Aspergillus fumigatus and a Bacillus amyloliquefaciens neutral protease preparation. These enzyme solutions were daily mixed was mixed in a dry TMR with 70% of rolled corn at a rate of 10 mL for 40 kg TMR. From day 8 to 14 chromium oxide (1 mg/kg) was mixed with the diet and from day 12 to 14 feces were collected to estimate total tract apparent digestibility and D-lactate concentration. Intake was recorded daily. Enzyme supplementation, regardless of enzyme type, increased (P < 0.05) DM (from 66.7% to 73.1% ± 2.01), OM (from 66.7% to 72.9% ± 2.03), and starch (from 74.7% to 81.8% ± 2.25) total tract apparent digestibility, and did not affect feces D-lactate concentration (1.26 vs 1.00 ± 0.200 mM for control and enzyme supplemented, respectively). So, any of the enzyme preparations tested (glucoamylases from Trichoderma reesei or Aspergillus fumigatus, or Aspergillus fumigatus glucoamylase n combination with the Bacillus amyloliquefaciens protease) increased by 9.5% starch digestibility.

scholarly journals Effects of Exogenous Glucoamylase Enzymes Alone or in Combination with a Neutral Protease on Apparent Total Tract Digestibility and Feces D-Lactate in Crossbred Angus Bulls Fed a Ration Rich in Rolled Corn

Animals ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 1077
Author(s):  
Maria Devant ◽  
Shukun Yu ◽  
Sandra Genís ◽  
Torben Larsen ◽  
Li Wenting
Keyword(s):  
Experimental Design ◽  
Aspergillus Fumigatus ◽  
Bacillus Amyloliquefaciens ◽  
Dry Matter ◽  
Lactate Concentration ◽  
Latin Square ◽  
Nutrient Digestibility ◽  
Neutral Protease ◽  
Apparent Digestibility ◽  
Beef Bulls

The aim of this study was to evaluate the effect of two glucoamylases (GA) and the combination of one GA with a neutral protease on apparent total tract digestibility in beef bulls fed a total mixed ration (TMR) rich in rolled corn. Sixteen Angus beef bulls (266 ± 4.9 kg of initial BW, and 182 ± 1.7 d of age) were distributed in 4 blocks, each block consisted of 4 animals balanced by BW. The experimental design was a 4 × 4 Latin square (4 blocks and 4 periods, 2 w per period). Four treatments were tested; (1) control, (2) GA preparation from Trichoderma reesei (TrGA); (3) GA from Aspergillus fumigatus (AfuGA); (4) AfuGA in combination with a neutral protease from Bacillus amyloliquefaciens (BamPro). Apparent total tract digestibility and fecal D-lactate concentration were analyzed. Enzyme supplementation, regardless of enzyme type, increased apparent total tract digestibility of dry matter (from 66.7% to 73.1% ± 2.01), and starch (from 74.7% to 81.8% ± 2.25), without affecting feces D-lactate concentration. Irrespective of glucoamylase type, glucoamylase supplementation improved apparent digestibility of dry matter and starch, and the addition of a protease did not have additional benefits on nutrient digestibility.

In vivo apparent digestibility in ponies given rolled, micronised or extruded barley

1999 ◽  
Vol 1999 ◽  
pp. 133-133 ◽  
Author(s):  
B M L McLean ◽  
J J Hyslop ◽  
A C Longland ◽  
D Cuddeford ◽  
T Hollands
Keyword(s):  
Dry Matter ◽  
Latin Square ◽  
Design Experiment ◽  
Apparent Digestibility ◽  
Processing Methods ◽  
Physical Processing ◽  
Adaptation Phase

Processed cereals are used routinely in diets for equines but little information is available on how physical processing affects the digestibility of cereals in equines. This study examines the effects of three physical processing methods (rolling, micronisation and extrusion) on the in vivo apparent digestibility of barley fed to ponies.Three mature caecally-fistulated Welsh-cross pony geldings, (LW 284kg ± 3.8kg) were used in a 3 x 4 incomplete latin square changeover design experiment consisting of four 21 day periods. Each period comprised a sixteen day adaptation phase and a five day recording phase when apparent digestibility in vivo was determined. Ponies were offered 4kg dry matter (DM) per day of either 100% hay cubes (HC) or one of three diets consisting of a 50:50 barley:hay cubes mix. The barley in the mixed diets was either rolled barley (RB), micronised barley (MB) or extruded barley (EB). Diets were offered in 2 equal meals per day fed at 09:00 and 17:00 hours respectively.

PREPARATIONS OF BACTERIAL PROTEASES FOR THE PRODUCTION OF PROTEIN HYDROLYSATES WITHOUT BITTERNESS

2020 ◽  
pp. 407-409
Author(s):  
A. Sereda ◽  
I. Velikoretskaya ◽  
D. Mineeva ◽  
N. Tsurikova
Keyword(s):  
Bacillus Subtilis ◽  
Serine Proteases ◽  
Culture Liquid ◽  
Protein Hydrolysates ◽  
Neutral Protease ◽  
Enzyme Preparations ◽  
Casein Hydrolysates

The effect of the neutral and serine proteases ratio in enzyme preparations (EP) on the bitterness of casein hydrolysates was studied. Bacillus subtilis 359 strain producing proteolytic complex with a serine to neutral protease ratio similar to Neutrase was selected from VNIIIPBT strains collection. The preparation from B. subtilis 359 culture liquid provides obtaining protein hydrolysates without bitterness.

scholarly journals 84 Effect of incorporating forage pea (c.v. Pisium sativum L.) into cereal hay on ruminal fermentation and apparent digestibility when fed to beef heifers

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 73-73
Author(s):  
Alex Pursley ◽  
Bill Biligetu ◽  
Tom Warkentin ◽  
Bart Lardner ◽  
Greg B Penner
Keyword(s):  
Beef Cattle ◽  
Factorial Design ◽  
Latin Square ◽  
Nitrogen Retention ◽  
Apparent Digestibility ◽  
Ruminal Fermentation ◽  
Microbial Protein ◽  
Beef Heifers ◽  
Inclusion Rate ◽  
Protein Supply

Abstract The objective was to evaluate the effect of inclusion rate of pea hay in barley and oat hay when fed to beef cattle. Six ruminally-cannulated heifers (407 ± 38 kg) were used in a 6 × 6 Latin square with a 2 × 3 factorial design and 25-d periods. Treatments included barley or oat hay blended with pea hay to achieve inclusion rates of 0, 15, or 30% (DM basis). Pea inclusion increased DMI (P = 0.03) by 0.75 kg/d, and generally reduced sorting (P ≤ 0.006) against NDF and ADF compared to the cereal-only treatments. Pea inclusion decreased CP digestibility by 2.87% relative to cereal-only treatments, but did not affect the predicted microbial protein supply or nitrogen retention (P ≥ 0.77). Pea inclusion did not affect total ruminal SCFA concentration, but increased the molar proportions of acetate and butyrate and decreased the molar proportion of propionate (P ≤ 0.01). While cereal type did not affect DMI (P = 0.36) or total SCFA concentration (P = 0.61), use of oat hay improved DM digestibility (67.73 vs. 63.22% for oat and barley, respectively; P < 0.001) and increased nitrogen retention (P = 0.03) when compared to barley. Overall, incorporating pea into cereal hay increased DMI, reduced CP digestibility, and altered ruminal fermentation.

scholarly journals Carbohydrate digestion by the domestic cat (Felis catus)

1977 ◽  
Vol 37 (3) ◽  
pp. 365-373 ◽  
Author(s):  
J. G. Morris ◽  
Jeanette Trudell ◽  
Terri Pencovic
Keyword(s):  
Latin Square ◽  
Basal Diet ◽  
Domestic Cat ◽  
Apparent Digestibility ◽  
Wheat Grain ◽  
Protein Nitrogen ◽  
Fine Grinding ◽  
Meat Diet ◽  
Maize Grain ◽  
Adult Cats

1. Three experiments were conducted on the ability of cats to utilize dietary carbohydrates. In two experiments, the digestibilities of carbohydrates were measured by the chromic oxide-marker technique using a balanced Latin-Square allocation of treatments: in the third experiment, the effect of age and diet on the activity of intestinal β-galactosidase (lactase) (EC 3.2.1.23) and β-fructofuranosidase (sucrase) (EC 3.2.1.26) of kittens was measured.2. In Expt 1 the digestibilities of six individual carbohydrates, glucose, sucrose, lactose, dextrin, raw maize starch and wood cellulose added to a meat-based basal diet were measured.3. In Expt 2, a similar meat-based basal diet was used and the effect of three processing methods (fine and coarse grinding, and cooking) on the apparent digestibility of the starch in maize and wheat grain was measured.4. In Expt 3 the effects of the inclusion of either 200 g lactose or 200 g sucrose/kg in an all-meat diet and of age on the β-galactosidase and β-fructofuranosidase activities of the small intestine of weanling kittens were measured.5. Adult cats efficiently (> 0.94) digested all six individual carbohydrates added to the diet with the exception of cellulose, which was indigestible. The digestibility coefficients of glucose, sucrose and lactose were significantly (P < 0.01) greater than that of starch. The inclusion of lactose caused diarrhoea in some cats and significantly (P < 0.01) reduced apparent digestibility of crude protein (nitrogen × 6.25) in the total ration.6. Fine grinding significantly enhanced the digestion of starch in wheat and maize grain, but the effect was greatest for maize grain. Cooking had a similar effect to fine grinding for wheat grain, but an effect intermediate between coarse and fine grinding for maize grain.7. Intestinal β-galactosidase activity decreased with age in kittens (71-106 d). Neither β-fructofuranosidase nor β-galactosidase activities were significantly affected by the addition of sucrose and lactose to the all-meat diet.

Effects of physically effective neutral detergent fibre content on dry-matter intake, digestibility and chewing activity in beef cattle fed total mixed ration

2015 ◽  
Vol 55 (2) ◽  
pp. 166 ◽  
Author(s):  
Jae Hyun Park ◽  
Kyoung Hoon Kim ◽  
Pyo Jam Park ◽  
Byong Tae Jeon ◽  
Mi Rae Oh ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Dry Matter ◽  
Bos Taurus ◽  
Mixing Time ◽  
Latin Square ◽  
Eating Rate ◽  
Total Mixed Ration ◽  
Neutral Detergent Fibre ◽  
Significant Difference ◽  
Chewing Activity

Our aim in this study was to determine the effects of physically effective neutral detergent fibre (peNDF) in a total mixed ration (TMR) on feed intake, digestibility and chewing activity in beef cattle. The experiment had a replicated 3 × 3 Latin square desig, using three fattening Hanwoo (Bos taurus coreanae) steers with ruminal cannulas. Steers were offered one of three diets (high, medium or low peNDF content) obtained by mixing the same TMR for different periods of time (5, 13 and 25 min). peNDF content of TMR was calculated as [total chewing time/NDF intake (kg)] × dietary NDF content, and the proportion of sample dry matter (DM) collected in a ≥1.18-mm sieve is commonly used as the physical effectiveness factor in the equation. The peNDF1.18 contents of the high, medium and low diets were 25.97%, 21.10% and 17.94% (P < 0.05). In addition, the proportion of particles collected on the 19-mm, 1.18-mm, and pan sieves changed linearly with an increase of TMR mixing time. Mean particle-size distributions of diets were 11.43, 24.11, 30.70 and 33.68 for 19 mm, 8 mm, 1.18 mm and pan, respectively. Eating rate and ruminating and chewing efficiencies were significantly decreased with reduced peNDF content, and DM intake was increased significantly (P < 0.05). Lower peNDF content reduced the total number of chews significantly (P < 0.05). Total time spent chewing associated with eating was not affected by peNDF contents. Digestibility of DM, crude protein and crude fat decreased with reducing peNDF content in the diet, but there was no significant difference among the three groups. These results indicated that peNDF affects intake, digestibility and chewing activity in beef cattle. Therefore, high levels of peNDF appears to improve TMR, as it can increase efficiency and may prevent ruminal disorders in Hanwoo steer.

Circadian variation in the apparent digetibility of diets measured at the terminal ileum in pigs

1980 ◽  
Vol 94 (2) ◽  
pp. 399-405 ◽  
Author(s):  
R. M. Livingstone ◽  
Barbara A. Baird ◽  
T. Atkinson ◽  
R. M. J. Crofts
Keyword(s):  
Feed Intake ◽  
Terminal Ileum ◽  
Fish Meal ◽  
Circadian Variation ◽  
Latin Square ◽  
Latin Squares ◽  
Apparent Digestibility ◽  
Feeding Methods ◽  
Soyabean Meal ◽  
Ad Libitum

SummaryCircadian variation in the apparent digestibility of diets having different physical characteristics was measured in samples taken from the terminal ileum using simple cannulae and marker ratios. Six pigs were used in three latin squares involving three iso-nitrogenous diets (30 g N/kg D.M.). Diet A was based on barley, weatings, soyabean meal and fish meal, diet B included barley, weatings and oats and diet C was purified. The allocation of diet provided 100 g D.M./kg Weg0·75/24 h and in each latin square a different pattern of feed intake was used; diets were given at intervals of either 1 or 12 h, or ad libitum.Differences in the digestibility of the diets were consistently distinguished by the technique. The circadian variation in digestibility was related to the type of diet and could be modified by changing the number and distribution of feeds per day. The results show that an understanding of the variation associated with different diets and feeding methods is necessary for optimizing the strategy for sampling from the terminal ileum.

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