63 Effect of a Novel Consensus Bacterial 6-phytase Variant on Mineral Digestibility and Bone Ash in Young Growing Pigs Fed Diets with Low and High Soluble Limestone

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 42-43
Author(s):  
Deepak E Velayudhan ◽  
Arun Kumar ◽  
Yueming Dersjant-Li
Keyword(s):  
Negative Impact ◽  
Negative Control ◽  
Growing Pigs ◽  
Factorial Analysis ◽  
Canola Meal ◽  
The Novel ◽  
Bone Ash ◽  
Negative Effect ◽  
One Way Anova ◽  
Positive Controls

Abstract The effect of a novel consensus bacterial 6-phytase variant (PhyG) on total tract digestibility (ATTD) of minerals and bone ash was evaluated in piglets fed diets with low and high soluble limestone (LSLM and HSLM, 69 and 92% solubility at 5 min). For each limestone, 8 diets were formulated: an inorganic phosphate-free negative control (NC) diet based on wheat, corn, soybean-meal, canola-meal and rice-barn [0.18% standardized total tract digestible (STTD) P and 0.58% Ca], NC supplemented with 250, 500, 1,000 or 2,000 FTU/kg of PhyG, or with monocalcium phosphate (MCP) to formulate 3 positive controls with 0.21, 0.27, 0.33% STTD P, and 0.64, 0.70 and 0.75% Ca, respectively. In total 128 pigs (12.8 ± 1.33 kg, n=8) were adapted for 14 d followed by 4 d of fecal collection. Femurs were collected on the last day. Data were analyzed as one-way ANOVA and means separated by Tukey test. In addition, a 2 × 4 factorial analysis was performed to test the effect of limestone solubility at different MCP levels in control diets, a 2 × 5 factorial analysis was performed to determine the effect of limestone solubility at different phytase levels. Phytase dose-response was analyzed by orthogonal polynomial. Across phytase diets, HSLM reduced (P < 0.05) ATTD Ca and P compared with LSLM. A consistent negative effect on ATTD P was also observed with HSLM in control diets. Across limestones, increasing phytase increased (P < 0.05) ATTD Ca and P in a linear or exponential manner. Limestone solubility had no impact (P = 0.69) on bone ash. PhyG linearly increased (P < 0.05) bone ash and 250 FTU/kg of PhyG maintained bone ash compared to PC. In conclusion, limestone solubility showed a negative impact on ATTD P and Ca, the novel consensus phytase improved ATTD P and Ca regardless of limestone.

60 Effect of a Novel Consensus Bacterial 6-phytase Variant on Mineral Digestibility and Bone Ash in Young Growing Pigs Fed Diets with Different Concentrations of Phytate

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 45-46
Author(s):  
Charmaine D Espinosa ◽  
Deepak E Velayudhan ◽  
Yueming Dersjant-Li ◽  
Hans H Stein
Keyword(s):  
Soybean Meal ◽  
Block Design ◽  
Growing Pigs ◽  
Canola Meal ◽  
Experimental Unit ◽  
The Novel ◽  
Bone Ash ◽  
Randomized Complete Block Design ◽  
Fecal Collection

Abstract An experiment was conducted to test the hypothesis that increasing levels of phytase increases mineral digestibility and bone ash by pigs fed diets containing 0.23%, 0.29%, or 0.35% phytate-P. Within each level of phytate, 5 diets were formulated based on corn, soybean meal, and canola meal to contain 0, 500, 1,000, 2,000, or 4,000 phytase units (FTU)/kg of a novel consensus bacterial 6-phytase variant (PhyG). In addition, 3 reference diets were formulated by adding a commercial Buttiauxella phytase (PhyB) at 1,000 FTU/kg to the 3 diets containing no PhyG. A randomized complete block design with 144 pigs (12.70 ± 4.01 kg), 18 diets, and 8 replicate pigs per diet was used. Pigs adapted to diets for 15 d followed by 4 d of fecal collection. Femurs were collected on the last day. Data were analyzed as a 3 × 5 factorial with 3 levels of phytate-P and 5 levels of phytase, and contrast statements were used to compare 1,000 FTU of PhyG with PhyB. Pig was the experimental unit. Diets containing 0.35% phytate-P had reduced (P < 0.01) apparent total tract digestibility (ATTD) of Ca, P, Mg, and K compared with diets containing 0.23% or 0.29% phytate-P, but inclusion of phytase increased (P < 0.01) ATTD of Ca, Na, and K (Table 1). Phytase increased ATTD of P and Mg, but to a greater extent in diets with 0.23 or 0.29% phytate-P than in diets with 0.35% phytate-P (interaction, P < 0.05). Phytase increased bone ash, but to a greater extent if there was 0.35 rather than 0.23 or 0.29% phytate-P in the diets (interaction, P < 0.05). PhyG increased ATTD of P more (P < 0.05) than PhyB. In conclusion, the novel consensus phytase is effective in increasing bone ash and ATTD of Ca, P, Na, Mg and K.

scholarly journals Growth performance and nutrient digestibility of growing and finishing pigs fed multienzyme-supplemented low-energy and -amino acid diets

2020 ◽  
Vol 4 (2) ◽  
pp. 602-615
Author(s):  
Kevin Jerez-Bogota ◽  
Cristian Sánchez ◽  
Jimena Ibagon ◽  
Maamer Jlali ◽  
Pierre Cozannet ◽  
...  
Keyword(s):  
Growth Performance ◽  
Bone Mineralization ◽  
Control Diet ◽  
Phase 1 ◽  
Negative Control ◽  
Nutrient Digestibility ◽  
Ash Content ◽  
Growing Pigs ◽  
Bone Ash ◽  
Diet Type

Abstract A study was conducted to determine the effects of supplementing corn–soybean meal-based diets with a multienzyme on growth performance, bone mineralization, apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of nutrients of growing pigs. A total of 276 pigs (body weight [BW] = 33.99 ± 4.3 kg) were housed by sex in 45 pens of 6 or 7 pigs and fed 5 diets (9 pens/diet) in a randomized complete block design. Diets were positive control (PC); and negative control 1 (NC1) or negative control 2 (NC2) without or with multienzyme. The multienzyme used supplied at least 1,800, 1,244, 6,600, and 1,000 units of xylanase, β-glucanase, arabinofuranosidase, and phytase per kilogram of diet, respectively. The PC diet was adequate in all nutrients according to NRC recommendations and had greater digestible P content than NC1 or NC2 diet by 0.134 percentage points. The PC diet had greater net energy (NE) and standardized ileal digestible amino acids (AA) content than NC1 diet by 3%, and than NC2 diet by 5%. The diets were fed in 4 phases based on BW: Phase 1: 34–50 kg; Phase 2: 50–75 kg; Phase 3: 75–100 kg; and Phase 4: 100–120 kg. Nutrient digestibility and bone mineralization were determined at the end of Phase 1. Overall (34–120 kg BW), pigs fed the PC and NC1 diets did not differ in average daily gain (ADG) and average daily feed intake. Pigs fed NC2 diet had lower (P < 0.05) ADG and gain-to-feed ratio (G:F) than those fed PC diet. Pigs fed PC diet had greater (P < 0.05) bone ash content and ATTD of P than those fed NC1 diet. The ATTD of GE for PC diet was greater (P < 0.05) than that for NC2 diet, and tended to be greater (P < 0.10) than that for NC1 diet. Multienzyme interacted (P < 0.05) with negative control diet type on overall ADG and AID of GE such that multienzyme did not affect overall ADG and AID of GE for the NC1 diet, but increased (P < 0.05) overall ADG and AID of GE for NC2 diet by 5.09 and 8.74%, respectively. Multienzyme did not interact with negative control diet type on overall G:F, bone ash content, AID of AA, and ATTD of nutrients. Multienzyme increased (P < 0.05) overall G:F, AID of methionine, ATTD of GE and P, and tended to increase (P = 0.056) bone ash content. The ADG, bone ash content, and ATTD of GE and P for the multienzyme-supplemented diets were similar to (P > 0.10) PC diet. Thus, NE and digestible AA and P can be lowered by ≤5% in multienzyme-supplemented diets without effects on growth performance and bone ash of pigs.

Influence of a novel consensus bacterial 6-phytase variant on mineral digestibility and bone ash in young growing pigs fed diets with different concentrations of phytate-bound phosphorus

2021 ◽  
Author(s):  
Charmaine D Espinosa ◽  
Maryane S F Oliveira ◽  
Deepak E Velayudhan ◽  
Yueming Dersjant-Li ◽  
Hans H Stein
Keyword(s):  
Growth Performance ◽  
Block Design ◽  
Nutrient Digestibility ◽  
Growing Pigs ◽  
The Novel ◽  
Bone Ash ◽  
Randomized Complete Block Design ◽  
Total P ◽  
Fecal Collection

Abstract A 20-d experiment was conducted to test the hypothesis that phytase increases nutrient digestibility, bone ash, and growth performance of pigs fed diets containing 0.23%, 0.29%, or 0.35% phytate-bound P. Within each level of phytate, 5 diets were formulated to contain 0, 500, 1,000, 2,000, or 4,000 phytase units (FTU)/kg of a novel phytase (PhyG). Three reference diets were formulated by adding a commercial Buttiauxella phytase (PhyB) at 1,000 FTU/kg to diets containing 0.23, 0.29, or 0.35% phytate-bound P. A randomized complete block design with 144 individually-housed pigs (12.70 ± 4.01 kg), 18 diets, and 8 replicate pigs per diet was used. Pigs were adapted to diets for 15 d followed by 4 days of fecal collection. Femurs were collected on the last day of the experiment. Results indicated that diets containing 0.35% phytate-bound P had reduced (P < 0.01) digestibility of Ca, P, Mg, and K compared with diets containing less phytate-bound P. Due to increased concentration of total P in diets with high phytate, concentrations, apparent total tract digestible P and bone ash were increased by PhyG to a greater extent in diets with 0.29 or 0.35% phytate-bound P than in diets with 0.23% phytate-bound P (interaction, P < 0.05). At 1,000 FTU/kg, PhyG increased P digestibility and bone P more (P < 0.05) than PhyB. The novel phytase increased (P < 0.01) pig growth performance, and pigs fed diets containing 0.35 or 0.29% phytate-bound P performed better (P < 0.01) than pigs fed the 0.23% phytate-bound P diets. In conclusion, the novel phytase (i.e., PhyG) is effective in increasing bone ash, mineral digestibility, and growth performance of pigs regardless of dietary phytate level.

scholarly journals The Effect of Mothers’ Inappropriate Smart Device Usage Habits on Preschoolers’ Cognitive Ability: Sequential Mediating Effect of Preschoolers’ Smart Device Immersion Tendency and Self-regulation

2021 ◽  
Vol 59 (2) ◽  
pp. 157-168
Author(s):  
Su Mi An ◽  
Min Ju Kang
Keyword(s):  
Cognitive Ability ◽  
Negative Impact ◽  
Pearson Correlation ◽  
Self Regulation ◽  
Mediating Effect ◽  
Descriptive Statistics ◽  
Smart Device ◽  
Mediation Model ◽  
Negative Effect ◽  
One Way Anova

The purpose of this study was to examine the pathways between mothers’ inappropriate smart device usage habits, preschoolers’ immersion tendency and self-regulation, and children’s cognitive ability. The subjects of the study were 308 preschoolers aged 4 and 5 years and their mothers in Seoul, Gyeonggi-do, Incheon, Gangwon-do, Daejeon, Busan, and Mokpo. Data were analyzed with descriptive statistics, t-test, one-way ANOVA, and Pearson correlation using the SPSS 25 program. A sequential mediation model was analyzed using the AMOS 22 program. Mothers’ inappropriate smart device usage habits were found to have a negative effect on the preschoolers’ self-regulation, and this association was mediated by the preschoolers’ smart device immersion tendency. These findings show the mechanisms through which mothers’ inappropriate smart device usage habits negatively affect preschoolers’ smart device immersion and self-regulation which, in turn, has a negative impact on cognitive ability

474 Comparison Between Copper Sulfate and Dicopper Oxide on Body Weight and Copper Accumulation on Growing Pigs

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 210-211
Author(s):  
Laia Blavi ◽  
David Solà-Oriol ◽  
Alessandra Monteiro ◽  
José Francisco Perez ◽  
Hans-Henrik H Stein
Keyword(s):  
Body Weight ◽  
Bone Mineralization ◽  
Phase 1 ◽  
Negative Control ◽  
Growing Pigs ◽  
Copper Accumulation ◽  
Cu Accumulation ◽  
Bone Ash ◽  
Dietary Treatments ◽  
Therapeutic Doses

Abstract There are several Cu sources in the market that have different effects on pigs. We hypothesized that supplementing therapeutic doses of Cu(I)oxide (Cu2O) in diets for growing pigs improves body weight (BW), bone mineralization, and reduces Cu accumulation in liver than supplementing pigs with Cu sulfate (CuSO4). Two-hundred growing pigs (initial BW: 11.5 ± 0.98 kg) were randomly allotted to 5 dietary treatments. Dietary treatments included the negative control (NC) diet without Cu supplementation, and 4 diets in which 125 or 250 mg/kg of Cu from CuSO4 or Cu2O were added to the NC diet. The experiment was divided into 4 phases. Pig weights were recorded on d 1 and at the end of each phase. Eight pigs per treatment were sacrificed last d of phases 1 and 4 to obtain samples of liver, spleen, and the right metacarpal. Pigs fed diets containing 250 mg/kg of Cu2O had greater (P < 0.05) BW at the end of phases 1 and 2 than pigs fed the NC diet, and tended (P < 0.10) to have greater BW at the end of phases 3 and 4 than pigs fed the other diets. Copper accumulation in liver and spleen increased with Cu dose, but at the end of phase 1, pigs fed 250 mg/kg CuSO4 had greater (P < 0.05) Cu concentration in liver and spleen than pigs fed 250 mg/kg Cu2O. Pigs fed diets containing 250 mg/kg Cu2O had greater (P < 0.05) quantities of bone ash and greater (P < 0.05) concentrations of Ca, P, and Cu in bone ash than pigs fed the NC diets or diets containing CuSO4. Therefore, supplementing diets for growing pigs with therapeutic doses of Cu2O improves BW and bone mineralization with less Cu accumulation in liver compared with pigs fed diets containing CuSO4.

scholarly journals Effects of a novel corn-expressed E. coli phytase on digestibility of calcium and phosphorous, growth performance, and bone ash in young growing pigs1

2019 ◽  
Vol 97 (8) ◽  
pp. 3390-3398 ◽  
Author(s):  
Laia Blavi ◽  
Cristhiam J Muñoz ◽  
Jonathan N Broomhead ◽  
Hans H Stein
Keyword(s):  
Growth Performance ◽  
Control Diet ◽  
Positive Control ◽  
Negative Control ◽  
Nutrient Digestibility ◽  
Growing Pigs ◽  
Left Femur ◽  
Bone Ash ◽  
Dietary Treatments ◽  
Total P

AbstractTwo experiments were conducted to test the hypothesis that a corn-expressed phytase increases growth performance, bone measurements, and nutrient digestibility by young growing pigs, if added to diets that are deficient in Ca and P. In Exp. 1, 60 pigs (initial BW: 10.78 ± 0.67 kg) were randomly allotted to 6 dietary treatments that included a positive control diet (PC; 0.70% total Ca and 0.60% total P) and a negative control diet (NC; 0.50% total Ca and 0.42% total P). Four additional diets were formulated by supplementing the NC diet with 250, 500, 1,000, or 1,500 phytase units (FTU)/kg. Diets were fed for 28 d and the individual BW of pigs on days 1 and 28 were recorded. Fecal samples were collected from days 25 to 27 to calculate apparent total tract digestibility (ATTD) of Ca and P. On the last day of the experiment, all pigs were euthanized, and the left femur was removed and analyzed for ash, Ca, and P. Results indicated that growth performance, ATTD of Ca and P, and bone ash measurements were reduced (P < 0.05) in NC fed pigs compared with PC fed pigs. However, growth performance, ATTD of Ca and P, and bone ash measurements were improved (linear and quadratic, P < 0.05) by including increasing concentrations of phytase to the NC diet. In Exp. 2, experimental procedures were similar to those used in Exp. 1. Forty-eight pigs (initial BW: 11.15 ± 0.85 kg) were randomly allotted to 6 dietary treatments in a 28-d experiment. Treatments included a PC diet, an NC diet, and 4 diets in which 500 or 1,000 FTU/kg of either the corn-expressed phytase or a commercial microbial phytase were added to the NC diet. Pigs fed the NC diet had reduced (P < 0.01) final BW, ADG, G:F, and bone ash concentrations compared with pigs fed the PC diet. When 500 FTU/kg phytase was fed, no differences were observed in growth performance or bone ash measurements between phytase sources, and there were no differences in growth performance among pigs fed 1,000 FTU/kg of either phytase source or the PC diet. However, regardless of concentration or source of phytase, pigs fed the PC diet had greater (P < 0.001) amount of bone ash, bone Ca, and bone P compared with pigs fed phytase diets. In conclusion, the corn-expressed phytase is effective in improving growth performance, Ca and P digestibility, and bone measurements in pigs fed diets that are deficient in Ca and P.

PD-L1 and survival in oral cavity squamous cell carcinoma

2020 ◽  
Vol 25 (4) ◽  
pp. 287-294
Author(s):  
S. I. Kutukova ◽  
N. P. Beliak ◽  
G. A. Raskin ◽  
M. S. Mukhina ◽  
Yu. V. Ivaskova ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Cavity ◽  
Cell Carcinoma ◽  
Tumor Cells ◽  
Squamous Cell ◽  
Immune Cells ◽  
Negative Impact ◽  
Progression Free Survival ◽  
Negative Effect ◽  
Effect Of Pd

Relevance. Prognostic value of PD-L1 expression in oral cavity squamous cell carcinoma (OCSCC) and its effect on survival is still controversial. It should be to determine the prognostic role of PD-L1 expression on tumor and immune cells of OCSCC and assess their effect on overall survival (OS) and progression-free survival (PFS).Materials and methods. A prospective study included 145 patients, first diagnosed with OCSCC. PD-L1 expression on tumor and immune cells, infiltrating tumor and its microenvironment, was assessed in all tumor samples by IHC, CPS was calculated. Cut-off values were determined by ROC analysis for identification of PD-L1 expression effect on OS and PFS.Results. Most patients with oral mucosa squamous cell carcinoma showed positive expression of PD-L1 on tumor (77.2%) and immune cells (92.4%). The median PD-L1 expression on tumor cells was 13.5% [1.0-40.0], the median PD-L1 expression on immune cells was 5.0% [1.0-11.0], and the median CPS – 18.0 [3.0-7.8]. Univariate and multivariate analyses revealed a significant negative effect of PD-L1 expression on immune cells ≤ 7% on OS (HR 0.66; 95% CI 0.45-0.93; p = 0.0498); PD-L1 expression in tumor cells ≤ 15% (HR 0.65; 95% CI 0.43-0.98; p = 0.0416) and CPS ≤ 21 (HR 0.62; 95% CI 0.44-0.92; p = 0.0183) for PFS. PD-L1 expression in tumor cells ≤ 6% (HR 0.71; 95% CI 0.47-1.08; p = 0.1096) and CPS ≤ 7 (RR 0.67; 95% CI 0.44-1.01; p = 0.0575) had a confident tendency to negative impact on OS.Conclusion. Positive PD-L1 expression in tumor and immune cells as well as CPS are effective additional factors in the prognosis of the disease course, OS and PFS in patients with OCSCC.

scholarly journals Relationships Among the Feeding Behaviors of a Mirid Bug on Cotton Leaves of Different Ages and Plant Biochemical Substances

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Haiyan Song ◽  
Zhaoke Dong ◽  
Lili Li ◽  
Zengbin Lu ◽  
Chao Li ◽  
...  
Keyword(s):  
Negative Impact ◽  
Leaf Age ◽  
Leaf Traits ◽  
Cotton Leaf ◽  
Young Plant ◽  
Feeding Behaviors ◽  
Probing Behavior ◽  
Negative Effect ◽  
Mirid Bug ◽  
The Relationship

Abstract Apolygus lucorum (Meyer-Dür) (Hemiptera: Miridae) tends to feed on young plant tissues. To explore the relationship between stylet probing behaviors of adult A. lucorum and conditions of cotton leaves, we conducted an experiment using electropenetrography (EPG). Behaviors were recorded on four cotton varieties, in relation to thickness and biochemical traits of differently-aged leaves. Cotton leaf age had a significant effect on the probing behavior of A. lucorum but cotton variety did not. One-day-old leaves of A. lucorum received the highest mean number of stylet probes (penetrations) per insect, and longest mean durations per insect of combined stylet probing or its components, cell rupture and ingestion behaviors. All of the leaf traits (thickness and biochemical substances) were similar among these four cotton varieties. Leaf thickness had a significantly negative effect on the same four variables above. Gossypol and tannin also had a negative impact on combined probing duration. Redundancy analysis showed that the four EPG variables were closely related to nutrient substances (amino acids, sugar, and water) while they had the opposite relationship with plant defense substances (gossypol and tannin). On cotton in the seedling stages, A. lucorum fed more readily on the youngest, thinnest leaves in our no-choice EPG experiments. Nutrients and chemical resistance substances determined the probing duration of A. lucorum. Our findings can contribute to better understanding of patterns of feeding and host consumption by A. lucorum, ultimately improving cotton resistance to A. lucorum.

scholarly journals Analysis of User Needs on Downloading Behavior of English Vocabulary APPs Based on Data Mining for Online Comments

Mathematics ◽  
2021 ◽  
Vol 9 (12) ◽  
pp. 1341
Author(s):  
Tinggui Chen ◽  
Lijuan Peng ◽  
Jianjun Yang ◽  
Guodong Cong
Keyword(s):  
Negative Impact ◽  
Positive Impact ◽  
User Needs ◽  
User Requirements ◽  
Functional Requirements ◽  
User Comments ◽  
Online Comments ◽  
Significant Negative Impact ◽  
Negative Effect ◽  
New Type

With highly developed social media, English learning Applications have become a new type of mobile learning resources, and online comments posted by users after using them have not only become an important source of intellectual competition for enterprises, but can also help understand customers’ requirements, thereby improving product functionalities and service quality, and solve the pain points of product iteration and innovation. Based on this, this paper crawled the online user comments of three typical APPs (BaiCiZhan, MoMoBeiDanCi and BuBeiDanCi), through emotion analysis and hotspot mining technology, to obtain user requirements and then the K-means clustering method was used to analyze user requirements. Finally, quantile regression is used to find out which user needs have an impact on the downloads of English vocabulary APPs. The results show that: (1) Positive comments have a more significant impact on users’ downloads behavior than negative online comments. (2) English vocabulary APPs with higher downloads, both the 5-star user ratings and the increase of emotional requirement have a negative effect on the increase in APP downloads, while the enterprise’s service requirement improvement has a positive effect on the increase of APP downloads. (3) Regarding English vocabulary APPs with average or high downloads, improving the adaptability and Appearance requirements have significant negative impact on downloads. (4) The functional requirements to improve products will have a significant positive impact on the increase in downloads of English vocabulary APPs.

scholarly journals Pandemic Prevention: Lessons from COVID-19

Encyclopedia ◽  
2021 ◽  
Vol 1 (2) ◽  
pp. 433-444
Author(s):  
Mario Coccia
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Infectious Diseases ◽  
Health System ◽  
Negative Impact ◽  
Lessons Learned ◽  
The Novel ◽  
Policy Responses

Coronavirus disease 2019 (COVID-19) is caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which appeared in late 2019, generating a pandemic crisis with high numbers of COVID-19-related infected individuals and deaths in manifold countries worldwide. Lessons learned from COVID-19 can be used to prevent pandemic threats by designing strategies to support different policy responses, not limited to the health system, directed to reduce the risks of the emergence of novel viral agents, the diffusion of infectious diseases and negative impact in society.

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