260 Growth Performance and Gut Integrity of Nursery Pigs Fed Diet with butyric Acid and Enzymatically Hydrolyzed Yeast Product

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 109-110
Author(s):  
Jinsu Hong ◽  
Hee Seong Kim ◽  
Rob Patterson ◽  
Tofuko A Woyengo

Abstract The objective of this study was to evaluate the effects of butyric acid (BA) and enzymatically hydrolyzed yeast product (EYP) on growth performance and gut health of nursery pigs. A total of 96 weaned pigs (initial body weight = 6.60 kg) were housed in 24 pens (4 pigs/pen) and fed 3 diets in a randomized complete block design. The diets were corn-soybean meal-based without or with 0.05% BA or 0.1% EYP. The diets were fed in 2 phases; Phase 1: d 0 to 7 and Phase 2: d 7 to 20. Growth performance and fecal score were determined by phase. One pig from in each pen was selected for measuring organ weights, gut permeability, and electrophysiological parameters of jejunum mounted in Ussing chambers. The selected pigs were sacrificed at a rate of 6 pigs (balanced for diets) per day from d 10 to 17. Dietary BA increased (P < 0.05) gain to feed ratio for d 7 to 20 by 5.8% and for entire study period by 15.8%. Fecal scores were unaffected by dietary BA or EYP. Dietary EYP increased (P < 0.05) weight of cecum as a proportion of live body weight by 36.4%. Dietary BA decreased (P < 0.05) the flow of fluorescein isothiocyanate-dextran from mucosal to serosal side of jejunum by 31.7%, implying that dietary BA reduced jejunal permeability. Jejunal short circuit current was decreased (P < 0.05) by dietary BA or EYP. However, jejunal trans-epithelial electrical resistance was unaffected by dietary BA or EYP. In conclusion, dietary BA improved feed efficiency and reduced jejunal permeability to fluorescein isothiocyanate-dextran, implying that it can improve the gut health of weaned pigs through reduced jejunal permeability to toxins. Dietary EYP increased caecum weight, implying that it can improve hindgut fermentation in weaned pigs.

2020 ◽  
Vol 33 (3) ◽  
pp. 159-171
Author(s):  
Paige K Isensee ◽  
Sarah E ◽  
Lindsey G Wichman ◽  
Autumn L Thoma ◽  
Young D Jang

Background: Creep feed is offered to suckling piglets to introduce solid feed and provide extra nutrients in late lactation. However, the effect of creep feed is inconsistent; there is little information about the effect of creep diet complexity on piglet performance. Objective: Two experiments were conducted to evaluate the effect of creep feed and its complexity on growth performance of suckling and weaned pigs. Methods: In Exp. 1, eight litters (average 19.9 ± 1.1 d of age; initial piglet weight: 6.74 ± 1.2 kg) were allotted to two dietary treatments considering breed, litter size and weight, as follows: no creep feed (n=3) and creep feed (n=5; offered for 8 days before weaning). At weaning (d 28 of age), the pigs were divided into three treatments (6 pigs/pen, 3 replicates; initial body weight: 9.66 ± 0.34 kg) balanced by gender, body weight, and breed, as follows: creep feed eaters, creep feed non-eaters, and no creep feed. In Exp. 2, two different types of creep feed were offered to suckling piglets (initial piglet weight: 3.79 ± 0.55 kg) in seven litters from d 12 of age (average 12.0 ± 1.3 d of age) to weaning (d 25 of age). Treatments were: HCF (n=4): highly-complex creep diet containing 3% fish meal, 2.4% blood meal, and 15% whey; and 2) LCF (n=3): lowly-complex creep diet without the mentioned ingredients. At weaning, only eater pigs were divided into 2 treatments (6 pigs/pen, 3 replicates; initial body weight: 7.53 ± 0.97 kg) balanced by gender, breed and body weight as follows: HCF eaters and LCF eaters. In both experiments, creep feed was mixed with 1% Cr2O3 to measure fecal color for eater/non-eater categorization and the pigs were fed a common nursery diet for 21 days. Results: In both experiments, there were no differences on piglet weaning weight and overall nursery growth performance among the treatments. In Exp. 2, the creep feed intake and percentage of eaters per litter were not different between the HCF and LCF treatments, whereas the HCF eaters tended to have a greater average daily gain (p=0.08) and gain to feed ratio (p=0.09) than the LCF eaters during d 7-14 postweaning. Conclusion: Creep feed did not affect overall piglet growth in suckling and nursery phases, but its complexity might affect pig growth in the early nursery phase.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 78-78
Author(s):  
Marcos E Duarte ◽  
James Tyus ◽  
Sung Woo Kim

Abstract This study was to investigate the effect of dietary supplementation with xylanase and probiotic on growth performance, and gut health of nursery pigs challenged with ETEC. Sixty-four weaned pigs (7.9 ± 0.4 kg) were allotted in a RCBD (2 x 2 factorial). ETEC (0, and ETEC, 6 x 109 CFU/mL) and synbiotic (0, and xylanase 10,000 XU/kg combined with Bacillus sp. 6 x 108 CFU/kg) were the factors. ETEC was orally inoculated on d 7. Growth performance were measured on d 7, 10, 15, and 21. On d 21, 48 pigs were euthanized for sampling to measure gut health parameters. Synbiotic increased (P < 0.05) ADG in P1 (53.5 to 96.0 g). Overall, ETEC reduced (P < 0.05) ADG (387.5 to 322.5 g) and G:F (0.810 to 0.706). ETEC increased (P < 0.05) fecal score from d 7 to 13, whereas synbiotic reduced (P < 0.05) it at d 9 and 11 in challenged pigs. ETEC increased (P < 0.05) MDA (0.259 to 0.818 μmol/mg), IL6 (2.96 to 4.30 pg/mg), ki-67+ (29.1 to 33.9%), and crypt depth (260 to 290 μm), whereas synbiotic tended to reduce TNF-α (1.05 to 0.87 pg/mg), protein carbonyl (3.13 to 2.51 nmol/mg), and IL6 (4.07 to 3.19 pg/mg); reduced (P < 0.05) crypt depth (290 to 260 μm), and ki-67+ (32.7 to 30.3%) and increased (P < 0.05) villus height (368.5 to 421.4 μm). ETEC reduced (P < 0.05) abundance of Veillonellaceae (7.11 to 3.02%), tended to reduce (P = 0.067) Clostridiaceae (1.1 to 0.55%), and Prevotellaceae (38.0 to 27.2%) and tended (P = 0.063) to increase Helicobacteraceae (34.5 to 49.5%). Collectively, ETEC reduced growth performance by affecting the microbiome, oxidative stress, and immune response. Synbiotic improve growth performance by enhancing gut health regardless of the challenge, whereas it reduced fecal score in challenged pigs.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 92-93
Author(s):  
Wenting Li ◽  
Jie Feng

Abstract Two studies were conducted with same set-up at a commercial facility to determine the effects of supplementing corn-SBM-based diets with or without a multi-enzyme on performance of nursery pigs. A total of 240 piglets (per trial) weaned at 25 d of age were randomly assigned to the 12 pens (10 barrows and 10 gilts per pen) pre-assigned with one of the experimental diets: a drug-free control diet containing 3000 ppm Zn and 1000 FTU phytase/kg with or without a multi-enzyme (4,000 U of xylanase, 150 U of amylase, 150 U of β-glucanase and 3000 U of protease/kg feed, n = 6). The diets were fed for 6 wks in 2 growth phases (phase 1: 0–2 weeks; phase 2: 3–6 weeks). Feed intake (ADFI), body weight (BW), body weight gain (ADG) were recorded by phase. Data were analysed by MIXED procedure of SAS (9.4) and significance was declared at P < 0.05. In phase 1, fecal scores were visually assessed daily by pen, ranging from 1 to 5 (1 = hard; 2 = firm; 3 = soft; 4 = very soft; 5 = watery feces). The frequency of diarrhea by pen were calculated by counting pigs with a diarrhea score of 3 or greater. In both trials, ADG during the first phase was improved by 30 (trial 1) or 40 g (trial 2) vs. control (P < 0.05). Final BW in piglets fed enzyme treatment was 1.4 (P < 0.05) or 1.2 kg (P > 0.05) heavier in trial 1 and 2, respectively vs. those fed the control diet. The ADFI was not affected by enzyme inclusion (P > 0.05). In addition, during the 1st phase, improvement in fecal condition (average 1.96 vs. 2.27; P < 0.05) and reduction in diarrhea incident (average 3.0 vs. 5.05%; P < 0.05) were observed in both trials when multi-enzyme treatment was fed to the piglets. In conclusion, the addition of multi-enzyme for weaned pigs improved growth performance and health condition.


2018 ◽  
Vol 98 (4) ◽  
pp. 868-874 ◽  
Author(s):  
E. Kiarie ◽  
C. Voth ◽  
D. Wey ◽  
C. Zhu ◽  
P. Vingerhoeds ◽  
...  

Benzoic acid (BA) supplement was evaluated as an alternative to antimicrobial growth promoter (AGP). Ninety-six piglets (21-d-old at weaning) were placed in pens (four piglets pen−1) based on body weight (BW) and allocated (n = 8) to either control corn–soybean meal diet or control + in-feed antibiotic (AGP, 220 mg chlortetracycline hydrochloride and 31.2 mg tiamulin kg−1) or control + 0.5% BA. Feed intake and BW were measured weekly. Fecal scores for incidence of diarrhea and fecal samples for apparent total tract digestibility (ATTD) of components were taken in week 2. One pig per pen was euthanized on day 14 for jejunal histomorphology and digesta for pH and short-chain fatty acids concentration. In weeks 1–6, pigs fed AGP and BA had better (P < 0.01) gain to feed ratio compared with control fed pigs. On day 12, pigs fed AGP and BA showed lower (P = 0.01) fecal score than control fed pigs. Pigs fed BA had (P < 0.05) higher ATTD of crude protein and taller villi compared with the control or AGP fed pigs. Pigs fed AGP showed higher (P = 0.05) lactic acid concentrations than pigs fed BA, however, none differed (P > 0.05) with control pigs. In conclusion, BA supported piglet growth performance to the same extent as AGP.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 46-47 ◽  
Author(s):  
Hailey Wooten ◽  
John J McGlone ◽  
Arlene Garcia ◽  
Amanda R Rakhshandeh ◽  
Anoosh Rakhshandeh

Abstract Previously we found that GRA reduces systemic inflammation in newly weaned pigs. Therefore, we evaluated the effects of GRA on growth performance and blood chemistry in newly weaned pigs, since inflammation impacts growth. A total of 209 pigs (BW 7.4 ±1.27 kg) were weaned at 26 ±1.5-d of age and assigned to a factorial CRD, with sex and treatment as main effects. Treatments included control (CON), GRA, ANT, and ANT+GRA (ANGA). Dexamethasone (0.2 mg/kg BW; i.m. injections) and Tylan 40 (0.12% of diet) were used as GRA and ANT, respectively. Pigs had free access to conventional corn-soybean diets according to a phase-feeding program. Feed intake, BW, ADG, measures of blood chemistry and hematocrit were determined over the 28-d post-weaning period. The repeated measures ANOVA (PROC MIXED) in SAS was used for statistical analysis. Interaction effects of sex and treatments were observed on BW and ADG (P = 0.03), but not on the gain-to-feed ratio (G:F). BW (kg) was higher in GRA gilts (10 ±0.08) than ANT gilts (9.7 ±0.06) and barrows (9.6 ±0.05), and CON barrows (9.4 ±0.09; P = 0.01). GRA gilts tended to be heavier than CON gilts (9.8 ±0.10; P = 0.06). ADG (kg) was higher in GRA gilts (0.40 ±0.014) than ANT gilts (0.34 ±0.019), CON barrows (0.29 ±0.016) and ANT barrows (0.36 ±0.012; P = 0.05). Overall G:F was higher in GRA pigs than CON pigs (0.63 vs. 0.56 ±0.020; P = 0.01) and tended to be higher than ANT pigs (P = 0.09). Creatinine levels tended to be lower in GRA pigs than ANT pigs (1.18 vs. 1.30 ±0.046 mg/dl; P = 0.07). Treatments had no effects on other measures of blood chemistry. Collectively, treatment with GRA effectively improves the growth performance of nursery pigs, especially gilts, during the nursery phase. GRA can be considered as an alternative to in-feed antibiotics for mitigating the adverse effects of weaning stress. USDA-NIFA-2017-68008-26304.


Author(s):  
M. Imran ◽  
S. Ahmed ◽  
Y. A. Ditta ◽  
S. Mehmood ◽  
Z. and M.W. Zia Rasool ◽  
...  

The effect of microencapsulated butyric acid (MEBA) on growth performance, ileal digestibility (ID) of protein, gut health and immunity was assessed in broilers. A total of 336 d-old Hubbard Classic broilers chicks were randomly assigned to four dietary treatments (Control, 0.25, 0.35 and 0.45 g/kg of MEBA). Each treatment was replicated three times with 28 birds/replicate. Feed intake, body weight gain, FCR, intestinal morphology, ID of protein and immunity was evaluated. At 35-d of age, three birds/replicate were randomly selected and slaughtered to collect blood, duodenal samples, and ileal digesta. The Improved body weight gain (P = 0.05), FCR (P less than 0.01), duodenal villous height (P less than 0.05) and ID (P less than 0.1) were observed; whereas, unresponsive treatment effect with respect to feed intake (P>0.5) and antibody titer against Newcastle disease (P>0.05) were noted. The results indicated that MEBA improves digestion and absorptive processes which consequently improved the broilers performance.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 158-159
Author(s):  
Hailey Wooten ◽  
Hwanhee Kim ◽  
Amanda Rakhshandeh ◽  
Anoosh Rakhshandeh

Abstract We previously found that i.m. injection of GRA, similar to in-feed antibiotics, improves measures of intestinal integrity, nutrient digestibility, and overall growth performance in nursery pigs. In addition, we have demonstrated that the mitigating effects of GRA on the stress of weaning is predominantly done by subsiding the inflammatory response that often accompanies early weaning. The objective of the current study was to explore the most practical methods for delivering GRA to newly weaned pigs. One-hundred and sixty-seven PIC pigs were weaned at 25 ±1.0 days of age (BW 7 ±1.2 kg), and assigned to fourteen treatments (3-4 pigs /pen; 3 pens/treatment). Seven treatments were tested within two sexes (gilts vs. barrows). The treatments were: 1) i.m. injection of GRA (two injections, 0.2 mg/kg BW at -1 and 3 d post-weaning), 2) two levels of in-water GRA (0.8 and 1.6 ppm), 3) in-feed GRA (2.5 and 5.0 ppm), 4) in-feed antibiotic (ANT, 100 ppm tylosin), and 5) control. Dexamethasone was used as GRA. Pigs had free access to in-feed or in-water GRA or ANT during the 1st-week post-weaning. Parameters of growth performance were measured weekly until the end of the nursery phase. A completely randomized design and repeated measurement ANOVA (PROC MIXED) in SAS were used for statistical analyses. In-water, in-feed, and i.m. GRA treatments resulted in growth performance outcomes comparable to that of in-feed ANT. Relative to CON pigs, both ANT and GRA improved ADG and G: F during the nursery phase (P &lt; 0.05). Among the delivery methods tested, 2.5 ppm of in-feed GRA resulted in superior ADG (GRA vs. CON; 0.29 vs. 0.20 kg/d) and G: F (0.63 vs. 0.40 ± 0.05), compared to the control group (P &lt; 0.02). Collectively, these results suggested that in-feed treatment is the best method for delivering GRA to newly weaned pigs.


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 175-176
Author(s):  
Joaquin J Sanchez Zannatta ◽  
L F Wang ◽  
Eduardo Beltranena ◽  
Aaron D Beattie ◽  
Rex N Newkirk ◽  
...  

Abstract Barley grain containing more fermentable starch or fiber might be an attractive energy source in weaned pig diets due to benefits on gut health. Barley rapidly-fermentable carbohydrates may serve as prebiotic and slowly-fermentable fiber may decrease diarrhea in weaned pigs. Steam-explosion processing may disrupt the fiber matrix of hulls, increasing slowly-fermentable fiber of barley. To explore, 220 pigs were fed 1 of 5 diets containing 60% cereal grain: 1) low-fermentable hulled barley (LFB); 2) LFB steam-exploded (LFB-E; 1.2 MPa, 120 s); 3) high β-glucan (10% DM) hull-less barley (HFB); 4) high amylose (17% DM) hull-less barley (HFA); or 5) low-fermentable wheat (LFW). Diets were fed starting 1-week post-weaning and formulated to provide 2.4 and 2.3 Mcal net energy (NE)/kg, 5.5 and 5.1 g standardized ileal digestible lysine/Mcal NE for phase 1 (day 1–14) and phase 2 (day 15–35), respectively. For the entire trial (day 1–35), average daily feed intake (ADFI) and average daily gain (ADG) of pigs did not differ among diets. Gain:feed (G:F) did not differ between LFB and LFW diets, but steam-explosion of hulled barley reduced (P &lt; 0.05) G:F. Feces consistency did not differ between LFB and LFW diets, but was better (P &lt; 0.05) for LFB than HFB, HFA and LFB-E diets. For phase 1, G:F of pigs was lower (P &lt; 0.05) for LFB-E diet than LFW diet. For days 22–28, LFB-E diet had greater (P &lt; 0.01) ADFI than HFA diet and tended (P = 0.09) to have a greater ADG than HFB diet. In conclusion, hulled or hull-less barley grain replaced wheat grain without affecting growth performance in weaned pigs. Hulled barley increased feces consistency. Steam-explosion of hulled barley did not increase growth performance of weaned pigs. Barley grain is an attractive energy source for weaned pigs for managing growth and feces consistency.


2021 ◽  
Vol 12 (7) ◽  
pp. 2962-2971
Author(s):  
Yuheng Luo ◽  
Jun He ◽  
Hua Li ◽  
Cong Lan ◽  
Jingyi Cai ◽  
...  

This study was conducted to compare the effect of raw (WB) or mixed fungi-fermented wheat bran (FWB) on the growth, nutrient digestibility and intestinal health in weaned piglets.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 77-78
Author(s):  
Yi-Chi Cheng ◽  
Marcos E Duarte ◽  
Sung Woo Kim

Abstract The objective was to determine the functional and nutritional values of Corynebacterium glutamicum Cell Mass (CGCM) on growth performance and gut health of newly-weaned pigs. Forty newly-weaned pigs (21 d of age; initial BW 7.1 ± 0.4 kg) were allotted to 5 dietary treatments based on randomized complete block design with sex and BW as blocks. The lysine broth of CGCM (CJ Bio, Fort Dodge, IA) was homogenized by using French press and dried to obtain lysed CGCM. Dietary treatments were: basal diet with lysed CGCM at 0, 0.7, 1.4, 2.1%, and with 1.4% intact CGCM. Experimental diets were formulated based on nutrient requirements (NRC, 2012) and pigs were fed based on 2 phases (10 and 11 d for each phase). Titanium dioxide (0.4%) was added to phase 2 diets as an indigestible external marker to calculate nutrient digestibility. Feed intake and BW were measured at d 0, 10, and 21. Pigs were euthanized on d 21 to collect proximal and distal jejunal mucosa to measure TNF-α, IL-8, MDA, IgA, and IgG concentrations. Diets and ileal digesta were collected to measure AID. Data were analyzed by SAS using MIXED, REG, and GLM procedures. Overall, increasing daily lysed CGCM intake increased (P &lt; 0.05) ADG (211 to 296 g) and ADFI (432 to 501 g). Increasing levels of lysed CGCM decreased (P &lt; 0.05) MDA and changed (quadratic, P &lt; 0.05) IgA (max: 4.90 ng/mg at 1.13%) and IgG (max: 3.37 ng/mg at 1.04%) in the proximal jejunal mucosa. Increasing daily lysed CGCM intake had quadratic effect (P&lt; 0.05) of protein carbonyl (max: 6.3 μmol/mg at 4.9 g/d). Lysed CGCM potentially benefits growth performance and gut health of newly-weaned pigs by reducing oxidative stress and increasing immune response.


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