278 Effect of Feeding CARS on Digestibility in Finishing Cattle Diets

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 128-128
Author(s):  
John Gibbons ◽  
Andrea K Watson ◽  
Galen E Erickson ◽  
Tyler Spore ◽  
Hannah Wilson ◽  
...  

Abstract An experiment was conducted to evaluate the digestibility of a novel liquid feed, Condensed Algal Residue Solubles (Veramaris, Blair, NE) in finishing cattle diets. Mass production of algae to harvest omega-3 fatty acids results in byproduct production of CARS (25.4% DM, 19.3% CP, 8.3% Fat, 9.96% Na on DM basis), made up of the de-oiled algae cells and residual fermentation substrates. The CARS product has generally recognized as safe (GRAS) status. Six ruminally and duodenally cannulated crossbred steers were assigned randomly to 1 of 3 treatments over 3 collection periods, for a 3 x 3 replicated Latin Square design. Treatments differed by increasing inclusion of CARS (0, 2.5, and 5% of diet DM) fed with CARS replacing steam flaked corn (72, 69.5, and 67% of diet DM as CARS inclusion increased). All diets contained 15% dry distillers grains, 8% alfalfa haylage, and 5% supplement. Cattle were dosed with 10 g of titanium dioxide per day. Duodenal and fecal samples were collected four times per day across four days and composited by period for each animal. Fecal samples were analyzed for titanium dioxide concentration to determine fecal output and diet digestibility. Data were analyzed with CARS inclusion and period as fixed effects and animal as a random effect. Orthogonal contrasts were used to test linear and quadratic effects of CARS inclusion. There were no significant differences for DM intake and OM intake between the different CARS inclusions (P ≥ 0.17), averaging 7.76 kg DM/d and 6.94 kg OM/d. Total tract DM digestibility was not affected by treatment (P ≥ 0.71) and averaged 73.0%. Total tract OM digestibility was also not different between treatments (P ≥ 0.93) and averaged 71.1%. Replacing steam flaked corn with CARS up to 5% of diet DM in finishing cattle diets did not affect diet digestibility.

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 138-138
Author(s):  
Thomas M Winders ◽  
Melissa L Jolly-Breithaupt ◽  
Hannah C Wilson ◽  
James C MacDonald ◽  
Galen E Erickson ◽  
...  

Abstract Two trials were conducted to evaluate the effects of biochar (0, 0.8, or 3% of diet dry matter) on diet digestibility and methane production in growing and finishing cattle diets. The growing diet consisted of 21% brome hay, 20% wheat straw, 30% corn silage, 22% wet distillers grains plus solubles (WDGS), and 7% supplement. The finishing diet consisted of 53% dry rolled corn, 15% corn silage, 25% WDGS, and 7% supplement. Biochar replaced fine ground corn in the supplement. Growing diets were evaluated over 6 periods in a switchback design, followed by the finishing trial with 3 periods in a crossover design using 6 steers (529 kg initial BW). Digestibility measures were taken over 4 d after at least 8 d of adaptation to diets followed by 2 d of gas emission measurements using headbox calorimeters. Statistical analysis included treatment and period as fixed effects and steer as a random effect with PROC IML of SAS used to generate coefficients for orthogonal contrasts. In the growing study, OM and NDF digestibility increased quadratically (P = 0.10) while OM digestibility tended to linearly decrease (P = 0.13) and NDF digestibility was not affected (P ≥ 0.39) by biochar inclusion in the finishing diet. Methane production (g/d) tended to decrease quadratically (P = 0.14) in the growing study and was decreased 10.7% for the 0.8% biochar treatment relative to the control. There were no statistical differences in methane production (g/d) in the finishing study (P ≥ 0.32). Methane production (g/kg DMI) from the 0.8% biochar treatment relative to the control was numerically reduced 9.5% and 18.4% in the growing and finishing studies, respectively (P ≥ 0.13). Although biochar is not an FDA-approved feed for cattle, initial research shows potential as a methane mitigation strategy in both growing and finishing diets.


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 129-130
Author(s):  
Aksel Wiseman ◽  
Tyler Spore ◽  
Mitch Norman ◽  
Hannah Wilson ◽  
James C MacDonald ◽  
...  

Abstract Six ruminally and duodenally cannulated steers were utilized in a 3 × 3 replicated Latin square experiment to determine duodenal fatty acid (FA) flow. Treatments consisted of 3 levels of Green Grass (GG, Sunseo Omega 3; Chungcheong Duk-Do, South Korea), a feed comprised of sesame meal, giant kelp, cassava, and sorghum, at 0, 15, and 30% of diet DM. Data were analyzed using the MIXED procedure of SAS with treatment and period as fixed effects and animal as a random effect. There were no differences in DMI, organic matter (OM) intake, total tract DM or OM digestibility (P ≥ 0.16). Intake of NDF and ADF increased linearly (P < 0.01) from 0 to 30% GG inclusion. Total tract digestibility of NDF was the poorest (P ≤ 0.02) for GG0 with no differences between GG30 and GG15 (P = 0.33). Total tract ADF digestibility was poorest for GG30 (P < 0.01) while GG0 and GG15 were not different (P = 0.17). Fatty Acid values were reported as relative abundance of the total FA present in duodenal samples. The lowest (P < 0.01) concentration of saturated FA was GG30 (70.3%) while GG0 and GG15 were not different (P = 0.83; 78.2%). The concentration of unsaturated, mono-unsaturated, poly-unsaturated, and trans FA responded quadratically (P < 0.01) with no difference between GG0 and GG15 and increasing to GG30. Omega-6 FA tended to decrease linearly (P = 0.06) from 0 to 30% GG inclusion (4.91 and 3.85 g/d, respectively). Omega-3 FA increased linearly (P < 0.01) from 0 to 30% GG inclusion (1.84 and 10.78 g/d, respectively). These data suggest increasing inclusion of GG up to 30% of diet DM does not affect DM or OM digestibility. Greater inclusions of GG resulted in more unsaturated and omega-3 FA concentrations in the duodenum.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 137-138
Author(s):  
Calvin Gibbons ◽  
Andrea K Watson ◽  
Galen E Erickson ◽  
Bradley M Boyd ◽  
Levi J McPhillips ◽  
...  

Abstract Algae oil production for Omega-3 fatty acid supplementation yields a byproduct called Condensed Algal Residue Solubles (CARS; 25.4% DM, 19.3% CP, 8.3% Fat, 9.96% Na on DM basis), de-oiled algae cells with residual fermentation substrates. This study evaluated the use of CARS in feedlot finishing diets. Crossbreed steers, (n=480) were blocked and stratified by initial body weight (BW) into 4 blocks, and assigned randomly to treatments. Treatments were designed as a 2 x 3 factorial with 3 inclusions of CARS (0, 2.5, 5% of diet DM) and 2 different base diets representing Northern and Southern Great Plains diets. The Southern diets contained steam flaked corn and dry distillers grains while the Northern diets had dry rolled and high moisture corn with wet distillers grains. CARS replaced corn in both diets. All blocks were harvested after 148 days on feed. Performance data were analyzed as a randomized block design with CARS inclusion, base diet, and interactions as fixed effects, BW block as a random effect and pen (n=48) as the experimental unit. Orthogonal contrasts were used to test linear and quadratic effects of CARS inclusion. There were no significant interactions between CARS inclusion and diet type (P ≥ 0.49). Main effects of CARS indicated positive quadratic responses for carcass adjusted ADG, G:F, 12th rib back fat, yield grade (P < 0.01; increasing to 2.5% inclusion, decreasing at 5%) and hot carcass weight was both linear and quadratic (P ≤ 0.01 and P ≥ 0.06 respectively; 969, 977, 935 as CARS increased). Linear decrease in DMI, final adjusted BW and ribeye area (P ≤ 0.01) as CARS increased. Cattle fed the Southern diets had greater ADG and G:F compared to Northern diets (P < 0.01). Including 2.5% CARS in the diet improved feed efficiency in both Northern and Southern based feedlot diets.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 137-138
Author(s):  
Mitch Norman ◽  
Zachary E Carlson ◽  
Fred H Hilscher ◽  
Galen E Erickson ◽  
Bruce Brodersen ◽  
...  

Abstract Algae production is increasing to supply the growing demand for Omega-3 fatty acids for aquaculture, human food and pet food; co-products from the algae industry could be a suitable feed ingredient for cattle feeding. A safety study was conducted to evaluate feeding algal biomass to cattle. Crossbreed cattle (20 steers and 20 heifers, 255 kg initial BW, SD=14) were individually fed 4 inclusions of condensed algal residue solubles (CARS; 0, 2.5, 5, 7.5 % of diet DM) displacing dry rolled corn in a finishing diet (62.5 to 70% corn and 15% distillers grains) for a minimum of 97 d. At harvest, organs were weighed and sampled. Blood was collected every 30 d. Performance data were analyzed as a RCBD with treatment, gender, and treatment by gender interactions as fixed effects, BW block as a random effect and individual animal as the experimental unit. Orthogonal contrasts were used to test for linear, quadratic and cubic responses due to CARS inclusion. Increasing CARS in the diet quadratically increased DMI and ADG (P ≤ 0.01). A linear increase was observed for G:F, NEm,and NEg as CARS increased in the diet (P < 0.01). All organ weights measured were within expected ranges for cattle, with 6 out of 27 having differences in weight due to treatment (P ≤ 0.05). Histopathology analysis of organs revealed no differences due to treatment (P ≥ 0.24). Out of 21 blood chemistry measures, 8 were affected by treatment (P ≤ 0.02). Nearly all blood chemistry parameters were within expected ranges for cattle. No adverse effects of feeding CARS were observed in hematology, blood chemistry, or histopathology analyses. The feedstuff CARS demonstrated to be a safe and efficacious feed ingredient for cattle diets and maximized HCW, ADG, and DMI when fed at 2.5 or 5% of the diet.


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 82-83
Author(s):  
Andres Tolosa Russi ◽  
Mike D Tokach ◽  
Robert D Goodband ◽  
Jordan T Gebhardt ◽  
Jason C Woodworth ◽  
...  

Abstract Due to packing plant closures or slow-downs, many producers needed to examine ways to reduce average daily gain (ADG) of finishing pigs. Therefore, a total of 1,080 pigs (L337 × 1050, PIC; initially 32.0 kg) were used in a 119-d trial to evaluate the effects of reducing dietary standardized ileal digestibility (SID) Lys and SID Trp:Lys ratio to slow growth of finishing pigs in a commercial setting. Pigs were randomly allotted in weight blocks to 1 of 4 dietary regimens with 27 pigs/pen and 10 replications/regimen. Pigs were fed a control regimen (100% of the estimated SID Lys requirement for pigs in this facility) formulated to contain 1.10, 1.01, 0.91, 0.83, 0.79, 0.71 and 0.67% SID Lys from 32 to 42, 42 to 51, 51 to 72, 72 to 85, 85 to 97, 97 to 112, and, 112 to 130 kg, respectively. Two other regimens contained 90 or 80% of the Lys estimate. These 3 regimes were formulated to a SID Trp:Lys ratio of 19% except for the last dietary phase that contained 17% SID Trp:Lys ratio. The fourth regimen contained 80% of the SID Lys estimate with 16% SID Trp:Lys in all phases. The statistical model included fixed effects of treatment, random effect of block, linear and quadratic effects of SID Lys and pairwise comparison of the two 80% treatments. Overall, decreasing SID Lys decreased (linear, P &lt; 0.01) ADG and final body weight (BW) and tended (P &lt; 0.10) to decrease gain:feed ratio (G:F). Reducing the Trp:Lys ratio decreased (P = 0.014) ADG and final BW compared to pigs fed diets with 80% SID Lys with higher SID Trp:Lys. In summary, decreasing SID Lys reduced ADG and feeding a reduced SID Trp:Lys ratio resulted in a further decrease in ADG of grow-finish pigs.


2020 ◽  
Vol 98 (6) ◽  
Author(s):  
Lourdes Llonch ◽  
Lorena Castillejos ◽  
Alfred Ferret

Abstract AbstractThe importance of fiber particle size in ruminal health is well known, but there are fewer studies to assess the requirements of physically effective NDF (peNDF) in beef cattle than in dairy cattle. The objective of this study was to establish the optimal peNDF proportion in high-concentrate diets fed to beef cattle, to reduce the risk of subacute ruminal acidosis. The experimental design was a replicated Latin Square 4 × 4, with four periods of 21 d. Treatments consisted of four diets with different peNDF proportions: 6.4%, 10.4%, 13.6%, and 15.4%, offered ad libitum as total mixed ration, and containing 15% barley straw and 85% concentrate. Diets, which differed in proportions of straw &gt; 4 mm (considered peNDF) and straw &lt; 4 mm, were manually mixed with concentrate. This concentrate was the same for all diets. A ruminal bolus was orally administered to each heifer for pH measurement. Intake, water consumption, intake by particle size, feed sorting, feeding behavior, behavioral activities, and rumen pH were recorded. Chemical composition and particle sizes of diets offered were assessed in the last week of each period. Data were analyzed using the MIXED procedure of SAS. Orthogonal contrasts determined the linear and quadratic effects of increasing peNDF proportion. T-test procedure determined whether heifers carried out sorting behavior. Particles &gt;4 mm linearly increased (P = 0.001), and particles &lt;4 mm linearly decreased (P = 0.001) as peNDF increased. Water consumption and feeding behavior were unaffected by treatment. As peNDF increased, intakes of DM and NDF linearly decreased (P = 0.001), whereas peNDF intake increased (P = 0.001). Intake of particles &gt; 4 mm linearly increased, whereas intake of particles &lt; 4 mm linearly decreased (P = 0.001) as peNDF increased. Diet 6.4% performed sorting for particles &gt; 4 mm (P &lt; 0.01), and diets 13.6% and 15.4% against particles &gt; 4 mm (P &lt; 0.01). Diet 10.4% tended to sort against particles &gt; 4 mm (P &lt; 0.10). Time spent ruminating linearly increased (P = 0.001) as peNDF increased. Diets did not differ in mean and minimum rumen pH, but time under rumen pH thresholds (5.8, 5.7, 5.6, and 5.5) linearly decreased as peNDF increased (P &lt; 0.05). The results suggested that the diet that best met the requirements of not compromising intake, limiting sorting behavior, and promoting time spent ruminating to reduce the number of hours under rumen pH thresholds, was the 10.4% diet.


2021 ◽  
Author(s):  
JEFFERSON RODRIGUES GANDRA ◽  
Alanne T. Nunes ◽  
Euclides R. Oliveira ◽  
Mávio S. J. Silva ◽  
Cibeli A. Pedrini ◽  
...  

Abstract Enzyme additives have been added to forage at ensiling to improve nutritive value. The aim of this study was to evaluate effects of adding exogenous enzymes to silage on fermentative losses and profile, aerobic stability, chemical composition, in vitro degradation, microbial quality, and nutrients intake and digestibility. Treatments were control (CON); addition of exo-1,4-α-glycosidase glucoamylase (GLU; Kerazyme 3035, Kera Nutrição Animal, Bento Gonçalves, Brazil); addition of β-glucan 4-glucanhydrolase (CEL); and GLU + CEL. Data from the silo experiment were analyzed using PROC MIXED of SAS, with fixed effects of glucoamylase and cellulase, and interaction effect between them. In vivo experiment analyses also included fixed effect of Latin Square and period, and random effect of animal within Latin Square. CEL increased (P ≤ 0.038) gas losses and effluents production, CEL and GLU decreased (P = 0.039) DM recovery compared to control but not differ from GLU+CEL. CEL silage had higher (P ≤ 0.021) starch and crude protein and in vitro digestibility of DM and NDF (P ≤ 0.032), while GLU had higher (P = 0.001) ADF. CEL showed lower (P = 0.012) ethanol content and higher (P = 0.02) anaerobic bacteria counts, while GLU showed higher (P = 0.012) lactate concentration and lower (P ≤ 0.002) counts of bacteria and fungi. Lambs fed with CEL presented higher (P ≤ 0.012) digestibility coefficients for DM, OM, CP and NDF. Decrease on DM recovery indicates no improvements on the nutritive value of silage. On the other hand, cellulolytic enzyme positively affected animal digestion.


Animals ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2928
Author(s):  
Taylor L. Richards ◽  
Alexandra Rankovic ◽  
John P. Cant ◽  
Anna K. Shoveller ◽  
Jennifer L. Adolphe ◽  
...  

Gastric emptying rate (GER) may impact diabetes and obesity in humans and could provide a method to reduce canine weight gain. Starch, the most common source of carbohydrates (CHOs) in pet food, is classified as rapidly or slowly digestible, or resistant to digestion. This study investigated starch source effects in commercial extruded dog foods on the GER of 11 healthy adult Siberian Huskies. Test diets were classified as traditional, grain-free, whole-grain, and vegan. Dogs received each diet once, a glucose control twice, and acetaminophen (Ac) as a marker for GER in a randomized, partially replicated, 6 × 6 Latin square design. Pre- and post-prandial blood samples were collected at 16 timepoints from −15 to 480 min. Serum Ac concentrations were assessed via standard spectrophotometric assays and fitted with a mathematical model to estimate parameters of GER. Parameter values were subjected to ANOVA, with period and treatment as fixed effects and dog as a random effect. More total emptying (p = 0.074) occurred at a faster rate (p = 0.028) in dogs fed the grain-free diet, which contained the lowest total starch (34.03 ± 0.23%) and highest resistant starch (0.52 ± 0.007%). This research may benefit future diet formulations to reduce the prevalence of canine weight gain.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 155-155
Author(s):  
Victor V Carvalho ◽  
Alexandre Perdigão ◽  
Tiago S Acedo ◽  
Guilherme S Vasconcellos ◽  
Luis Fernando Tamassia

Abstract Our objective was to evaluate the effects of feeding increasing exogenous carbohydrases levels on cattleˈ performance, when fed a 90-d feedlot finishing diet. A total of 240 Nellore bulls (IBW = 389 ± 27 kg) were distributed in 24 pens (10 animals/pen), in a randomized complete block design (6 pens/treatment). The basal diet was composed by 15% sugarcane bagasse, 72.8% ground corn, 7.6% soybean meal and 4.6 % mineral-vitamin premix (DM basis). The treatments were: 1) control: No addition of carbohydrases blend; 2) Blend1, in g/animal/d: 1 g of Ronozyme® WX (xylanase) + 3.75 g of Ronzyme® VP (beta-glucanase); 3) Blend2, in g/animal/d: 2 g of Ronozyme® WX + 7.5 g of Ronzyme® VP and 4) Blend3, in g/animal/d: 4 g of Ronozyme® WX + 15 g of Ronzyme® VP. The animals were fed once a day and had free access to fresh water. Statistical analysis was performed using the MIXED procedure of SAS 9.4, and comparisons were performed out by orthogonal contrasts, which compared the control and treatments, and the linear and quadratic effects of the increasing carbohydrases levels. Statistical significance was declared at P &lt; 0.05, and tendencies considered when 0.05 &lt; P &lt; 0.10. Animals fed carbohydrases had greater (P = 0.045) ADG (1.59 vs. 1.51 kg/d) and tended to have greater (P = 0.069) final BW (533.4 vs. 527.7 kg) compared with control; although no difference were observed on these variables among carbohydrases levels. Feeding carbohydrases increased DM intake, expressed either as kg/d (10.17 vs. 9.92; P = 0.019) and as percentage of BW (2.20 vs. 2.16 %; P = 0.038), compared to control group, respectively. However, no difference was observed among treatments on G:F, and dressing percentage. In conclusion, the addition of carbohydrases blend can improve performance of finishing cattle.


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 173-173
Author(s):  
Aileen Joy Mercado ◽  
Terry Weeden ◽  
Emily Otto-Tice ◽  
Huyen Tran ◽  
Murali Raghavendra Rao ◽  
...  

Abstract A total of 270 pigs (PIC 1050 x DNA 600, 6.0 kg) were used in a 42-d nursery trial to determine the optimum SID threonine to SID lysine (SID Thr/Lys) ratio for young pigs. Pigs were sorted by sex and initial BW, and were allotted to each pen (3 barrows, 3 gilts). Pens were then randomly assigned to diets containing incremental levels of SID Thr/Lys ratio (0.55, 0.60, 0.65, 0.70, 0.75), achieved using THRPRO 80 (CJ America, CA) as the source of L-threonine. Each treatment consisted of 45 piglets and 9 replicates per treatment. There were 3 dietary phases (2 weeks each) and the same levels of SID Thr/Lys were tested for each phase. All performance data (ADG, ADFI, and Gain to Feed (G:F)) were analyzed using Proc Mixed in a RCBD model with SID Thr/Lys ratio as fixed effect and weight block as random effect. Orthogonal contrasts were performed to test for linear and quadratic effects of SID Thr/Lys ratio on all performance parameters. Final BW averaged around 20.8 kg (±0.6) and was not influenced by SID Thr/Lys ratio (P&gt;0.20). It was however lower than previously observed from previous nursery batches and might be due to pig infection with Lawsonia intracellularis and Streptococcus suis. A linear trend on ADG was observed during the last phase of nursery (P=0.10) but this response was not significant for the overall study (P&gt;0.15). SID Thr/Lys ratio had no significant effect on ADFI during any phase or overall experimental period (P&gt;0.20). Increasing SID Thr/Lys ratio, however, linearly increase G:F of nursery pigs overall (P&lt; 0.05). The results of this study suggest that there is value in terms of increasing efficiency of gain with higher level of SID Thr/Lys as compared to current NRC recommendation (0.59), especially under disease challenge conditions.


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